Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| phage beta interaction with vibrio cholerae. | the role of temperate phage beta in determining the serology and eltor-lytic phage sensitivity in vibrio cholerae was investigated. the only serological change found in six host strains was a change to roughness. this was accompanied by failure to adsorb several of the lytic phages. various phage-sensitivity changes were induced by phage beta in two hosts at the post-adsorption level. in strain hp47, three types of progeny were obtained of which one was universally resistant to lytic phages. the ... | 1984 | 6492121 |
| studies on the enteropathogenic mechanism of non-o 1 vibrio cholerae isolated from the environment and fish in toyama prefecture. | enteropathogenic mechanisms of non-o 1 vibrio cholerae were investigated using strains from the environment and those from fish in toyama prefecture. none of the 93 non-o 1 v. cholerae strains produced a detectable level of choleratoxin-like-enterotoxin (ct-like-enterotoxin) in syncase medium, while 23 strains showed a distinct fluid accumulation in the rabbit ileal loop test (ril). these ril-positive strains neither produced ct-like-enterotoxin in vitro in the other four kinds of media which ar ... | 1984 | 6493071 |
| lysogenicity and prophage type of the strains of vibrio cholerae o-1 isolated mainly from the natural environment. | attention has recently been given to the increasingly frequent detection of atypical vibrio cholerae o-1 in the natural environments throughout the world. lysogenicity of v. cholerae o-1, mainly isolated from environmental sources in nine areas, including the united states, was studied by electron microscopy and a cross-lysis test between bacteria and the culture supernatants. a total of 38 strains isolated in texas in 1973 and in louisiana in 1978 were lysogenic, whereas there were no lysogenic ... | 1984 | 6496453 |
| simultaneous classical and el tor cholera in bangladesh. | cholera appears annually in bangladesh, with peak incidence from september through december and april through june. after being absent for a decade, the classical biotype of vibrio cholerae reappeared in commilla and dhaka on september 1 and 3, 1982, and soon thereafter appeared in many other districts. cases due to the newly emerged classical biotype of v. cholerae rapidly surpassed those due to the el tor biotype. before 1970, classical v. cholerae had been responsible globally for both end ... | 1984 | 6501815 |
| a new enterotoxin produced by vibrio cholerae o1. | 1984 | 6501817 | |
| classical vs el tor cholera: a prospective family study of a concurrent outbreak. | 1984 | 6501822 | |
| entrance of cholera enterotoxin subunits into thymus cells. | analysis of the staining of cholera enterotoxin on the surface of cells with specific antibodies against each subunit of cholera enterotoxin, using a fluorescence-activated cell sorter and electron microscopy, showed that not only subunit a but also subunit b penetrates the cell membrane. the detection of subunits inside the cell was facilitated by the use of saponin, an agent that increases membrane permeability. | 1984 | 6501862 |
| inhibitor to the cell-bound haemagglutinin of vibrio cholerae. | an inhibitor to cell-bound ha was found to be produced at the non-haemagglutinating phase of the culture cycle by a classical vibrio strain which produced a cell-bound ha early and transiently during its growth. the ha-negative filtrate obtained from the late log-culture was found to inhibit the cell-bound ha activity produced by the same vibrio strain. it was also found to be produced early in shaking cultures at 37 degrees c and to mask the activity of early cell-bound ha in whole culture test ... | 1984 | 6505790 |
| [variability of vibrio cholerae as affected by subbacteriostatic concentrations of antibiotics]. | the effect of subbacteriostatic concentrations of some antibiotics on variation of the features characterizing the serovar and biovar of the cholera germ was studied on 10 strains of v. cholerae e1 tor of the inaba serovar having the typical properties. it was shown that even a single exposure to the subbacteriostatic concentrations of some antibiotics resulted in formations of clones differing from the parental strains by a number of significant features, such as the antigenic structure, phage ... | 1984 | 6508252 |
| response of toxigenic vibrio cholerae 01 to physico-chemical stresses in aquatic environments. | the survival and growth of toxigenic vibrio cholerae 01 in water under various conditions of salinity, ph, temperature and cation composition and concentration were studied in an extensive series of laboratory experiments. inter- and intra-strain variation in stress response (of 01 and non-01 strains) and the ability of v. cholerae to adapt to stressful environments were also studied. toxigenic v. cholerae 01 were able to survive for at least 70 days at 25 degrees c in solutions of sea salt. the ... | 1984 | 6512251 |
| [the role of intestinal alkaline phosphatase in cholera toxin-induced secretory diarrhea]. | 1984 | 6513120 | |
| intestinal antibodies in rats following exposure to live vibrio cholerae. | indirect enzyme-linked immunosorbent assay methods were used to characterize the primary, secondary and tertiary antibody responses of rats to the lipopolysaccharide (lps) and heat-sensitive surface-associated (hssa) antigens of v. cholerae in the major immunoglobulin classes of serum, intestinal mucus and bile following intestinal injections of live organisms. antibody production following the first injection was limited to the igg and igm classes of serum and the iga class of bile but a second ... | 1984 | 6517765 |
| occurrence of transferable multi-drug resistance in vibrio cholerae-o1 in an endemic area. | 1984 | 6519698 | |
| cell-bound haemagglutinin (cha) of v. cholerae 01 as protective antigen. | the study of immunogenicity of cell-bound haemagglutinin (cha) of vibrio cholerae e1 tor in mice revealed that the cha was a good antigen when it was adsorbed onto the surface of sheep red blood cells and given orally to mice. the antigen not only induced high levels of various class antibodies which sustained in the intestinal tracts for a long period of time (longer than 6 months) but also the antibodies were protective against homologous cholera challenge. the degree of protection seems to co ... | 1984 | 6523176 |
| [action of vibrio cholerae enterotoxin on microorganisms]. | the damaging of v. cholerae enterotoxin on anaerobic dehydrogenases of different species of opportunistic microorganisms has been revealed. experiments, carried out both in vivo and in vitro, have shown that under certain conditions this enterotoxin may act as one of the factors of the intra- and interspecific antagonism of bacteria. | 1984 | 6524163 |
| [assessment of the importance of certain properties for the virulence of vibrio cholerae and the detection of their correlations]. | the study of 65 v. cholerae strains and mutants with different biological characteristics has revealed that their virulence directly correlates with their sensitivity to diagnostic cholera phages, lecithnase activity, viability and inversely correlates with their hemolytic activity. the role of lecithinase activity in a number of properties linked with cholerigenicity has been shown. inverse correlation between the hemolytic activity of v. cholerae and some properties of this organism has been d ... | 1984 | 6524164 |
| a tetraantennary glycopeptide from human tamm-horsfall glycoprotein inhibits agglutination of desialylated erythrocytes induced by leucoagglutinin. | complex-type glycopeptides from human tamm-horsfall glycoprotein were fractionated by affinity chromatography on leucoagglutinin-agarose. an oligosaccharide species was retained by the lectin-gel, suggesting that it contains an alpha-mannose residue of the trimannosyl core substituted at c-2 and c-6 positions with beta-n-acetylglucosamine, as in tetraantennary oligosaccharides. the carbohydrate composition supported this branching pattern. the agglutination of neuraminidase-treated human erythro ... | 1984 | 6525453 |
| [lysogeny studies of vibrio cholerae nag]. | to establish the presence of phages specific for v. cholerae nag, 673 strains, 166 water samples from ponds and sewage and 674 fecal samples from patients with acute intestinal diseases have been studied; as a result, 54 phage races have been isolated. the occurrence of true lysogeny in v. cholerae nag has been found to be infrequent (0.9-10% among newly isolated strains). pseudolysogeny has been detected in 0.75% of the strains under study. of the total number of truly lysogenic strains of v. c ... | 1984 | 6528780 |
| [potentials and conditions for the reversion of pathogenic properties of the causative agent of cholera in an experiment]. | the passage of v. cholerae noncholerigenic strains and their mutants, both in vitro and in vivo, has demonstrated that strains in which one of such properties as mobility, viability, adhesive, lecithinase and neuraminidase activities, is sharply decreased or lost, are still capable of reversion to cholerigenic forms. v. cholerae strains which have lost two or more of these properties, as well as strains having stable hemolytic activity determined by greig's test, seem to be incapable of such rev ... | 1984 | 6528781 |
| a longitudinal study of diarrhoea among children in calcutta communities. | a longitudinal study was conducted to determine the profile of diarrheal diseases in a group of 383 children below 5 years of age in 2 typical slums of calcutta (india). the overall annual incidence of diarrheal illness in these children during the 1st year of the study was found to be 1.1/child. however, the incidence was higher (1.9/child) in children below 2 years of age and declined progressively with advancing age. most (99.5%) of the diarrheal episodes were mild in nature and not a sing ... | 1984 | 6530269 |
| a clinical study of vibrio cholerae 01 in singapore related to environmental factors. | 1984 | 6530534 | |
| o-serogrouping scheme for mesophilic aeromonas strains. | the o-antigens of 307 strains of mesophilic aeromonas including 227 a. hydrophila and 80 a. caviae were studied and 44 o-serogroups defined among them. the presence of heat-labile masked antigen, which inhibits o-agglutination, was observed in some strains. as all the o-antisera prepared with these mesophilic aeromonas strains contained some r-antibody, all diagnostic o-antisera must be absorbed with r-organisms before use. some of the o-antigens were found to be identical or closely related to ... | 1984 | 6536785 |
| phosphate repression of phage protein synthesis during infection by choleraphage phi 149. | a synthetic medium for choleraphage phi 149 growth, in which the concentration of phosphate ions plays a significant role, has been defined. upon infection, choleraphage phi 149 dna binds to the cell membrane at three to four sites. the host macromolecular syntheses are shut off by 10 min after infection and the synthesis of phage-specific dna is detectable after 20 min of infection. the phage utilizes primarily the host dna degradation products for its own dna synthesis. when added during the f ... | 1984 | 6540006 |
| procholeragenoid: a safe and effective antigen for oral immunization against experimental cholera. | the immunogenicity and safety of procholeragenoid, a minimally toxic, heat-induced aggregate of cholera toxin (ct), were studied in enterically immunized rats and dogs. although 99% less toxic than ct, procholeragenoid was only slightly less efficient in causing jejunal anti-ct responses in rats; in contrast, choleragenoid, the nontoxic b subunit pentamer of ct, was much less effective. the immunogenicity of procholeragenoid was due almost entirely to its large-molecular-weight components (mw = ... | 1983 | 6602094 |
| transferable plasmid-mediated drug resistance among non-o1 vibrio cholerae and rough strains of vibrio cholerae from tamilnadu, india. | a total of 289 non-o1 vibrio cholerae (nvc) strains and 20 rough v. cholerae (rvc) strains isolated in an endemic area were tested for antibiotic resistance and for transferable r-plasmids. twenty three per cent of nvc and 40% of the rvc isolates were found to be resistant to one or more drugs. eight nvc and four rvc strains possessed multiple drug resistance, varying from four to eight drugs. the common spectrum found in nvc isolates were chloramphenicol and streptomycin (cs) or chloramphenicol ... | 1984 | 6607275 |
| vibrio cholerae bacteraemia in a newborn infant. a case report. | a 6-day-old black male infant presented with diarrhoea and biochemical evidence of severe electrolyte imbalance. despite treatment with intravenous fluids and antibiotics, he died within 24 hours of admission. enterotoxigenic vibrio cholerae, biotype e1 tor, serotype inaba, was isolated from the blood. the significance of this finding is discussed. | 1983 | 6612541 |
| the effect of neuraminidase on the relative surface charge-associated properties of rat red blood cells of different ages. | approx. 70% of the sialic acid on the rat erythrocyte surface is susceptible to cleavage by neuraminidase (vibrio cholerae). neuraminidase treatment results in a reduction in the partition coefficient (k) of the red cells in a charged dextran-poly(ethylene glycol) aqueous phase system and in the electrophoretic mobility of the cells. countercurrent distribution of rat neuraminidase-treated erythrocytes, containing 59fe-labeled mature red cells of distinct age, indicates that (a) the electrophore ... | 1983 | 6615837 |
| synthesis of phage-specific transfer rna molecules by vibriophage phi 149. | 32p-labelled trna was isolated from uninfected and phage phi 149-infected vibrio cholerae cells. these trna preparations were then hybridised with dna isolated from phage phi 149. significant hybridisation was observed only with trna from phage phi 149-infected cells. this strongly suggests that infection of classical vibrio with phage phi 149 results in the synthesis of phage-specific trna molecules. | 1983 | 6617890 |
| molecular epidemiological studies of united states gulf coast vibrio cholerae strains: integration site of mutator vibriophage vca-3. | environmental and clinical vibrio cholerae o-1 strains isolated from the u.s. gulf coast region were found to be lysogenic for a vibriophage which we have designated vca-3. comparison of vca-3 with the previously described vibriophages vca-1 and vca-2 has shown that vca-1 and vca-3 are homoimmune, have extensive sequence homology, but have markedly different restriction endonuclease digestion patterns. vca-3 was found to randomly integrate into the v. cholerae rv79 chromosome and to introduce st ... | 1983 | 6618665 |
| determination of serum bactericidal activity with the aid of luminous bacteria. | nonmarine luminous bacteria belonging to the genus vibrio cholerae were extremely sensitive to the bactericidal activity of human serum. luminous bacteria incubated in a medium containing serum showed a decrease in their in vivo luminescence that was directly proportional to the decrease in the viable count and was a function of the serum concentration. both immunoglobulins and the complement system were required to exert the serum bactericidal activity. serum lacking immunoglobulins or certain ... | 1983 | 6619281 |
| biological consequences of neuraminidase deficiency in newcastle disease virus. | a second-step revertant (l1) of a temperature-sensitive mutant (c1) of newcastle disease virus agglutinated erythrocytes normally but had less than 3% of the wild-type (strain av) levels of neuraminidase activity. revertant l1 had seven times more virion-associated n-acetylneuraminic acid (nana) than strain av. nana residues on purified virions were specifically labeled with periodate and tritiated borohydride. analyses of radiolabeled l1 virions on sodium dodecyl sulfate-polyacrylamide gels sho ... | 1983 | 6620458 |
| [adhesive properties of vibrio cholerae strains and mutants with varying biological characteristics]. | v. cholerae strains and mutants devoid of adhesive activity and possessing very high adhesive activity were found in the collection under study. as a rule, cholerigenic strains possessed high or moderate adhesive activity, while in noncholerigenic strains the coefficient of adhesiveness varied over a wide range. the existence of a definite pronounced correlation between lecithinase activity and adhesiveness was established. hemolysin-producing strains were found to possess high adhesive activity ... | 1983 | 6624312 |
| spectrum of vibrio infections in a gulf coast community. | infection with vibrio species was diagnosed in 23 patients over a 10-year period in a gulf coast community. species isolated were vibrio vulnificus, v. cholerae, v. parahaemolyticus, and v. alginolyticus. clinical presentations included diarrhea, wound infection, and bacteremia. gastrointestinal tract infection was infrequently diagnosed, despite the routine use of stool culture media appropriate for vibrio isolation. bacteremic infections were severe and occurred only in patients with underlyin ... | 1983 | 6625378 |
| removal of o-acetylated sialic acids from rat colonic epithelial glycoproteins by cell-free extracts of rat faeces. | sterile, cell-free, extracts of freshly defaecated wistar rat faeces in a ph 7.0 "minimal medium" contain neuraminidase(s), capable of removing sialic acids both with and without side-chain substituents from bovine submandibular mucin and rat colonic epithelial glycoproteins, and an esterase which removes o-acetyl substituents from the side chain of sialic acid residues. studies of the removal of sialic acids from bovine submandibular mucin and rat colonic epithelial glycoproteins indicated that ... | 1983 | 6627098 |
| duplication and amplification of toxin genes in vibrio cholerae. | vibrio cholerae strains of the classical biotype all contain two widely separated copies of the cholera toxin operon ctxab. in contrast, ei tor strains containing multiple copies of ctx have their copies arranged on large tandem repeats which are either 7 or 9.7 kb in length. the variation in size among these large tandem duplications was due to a difference in the copy number of a smaller, 2.7 kb, tandemly repeated sequence (rs1) that is located at the novel joint of these duplications, as well ... | 1983 | 6627396 |
| a neuraminidase from streptococcus sanguis that can release o-acetylated sialic acids. | the naturally occurring sialic acids can have different types of n- and o-substitutions, resulting in more than 20 known isomers and compounds. most methods for the detailed study of these various sialic acids require that the molecules be first released from their alpha-glycosidic linkage. when mild acid hydrolysis is used for this purpose, significant destruction of o-substituent groups occur. on the other hand, the presence of o-substituent groups renders the sialic acid molecule partially or ... | 1983 | 6630194 |
| monoclonal antibodies against group- and type-specific lipopolysaccharide antigens of vibrio cholerae o:1. | hybrid cell lines producing monoclonal antibodies against the o-antigenic determinants of vibrio cholerae o:1 have been established. the specificity of the antibodies was ascertained by enzyme-linked immunosorbent assay inhibition experiments by using lipopolysaccharides from v. cholerae o:1 strains and type strains of groups o:2 and o:21. the anti-a antibody was of the immunoglobulin m (igm) class, whereas the anti-b and -c antibodies were igg3. the antibodies had a good agglutinating capacity ... | 1983 | 6630437 |
| [the antibacterial effect of niridazole on vibrio cholerae strains]. | 1983 | 6634168 | |
| photodynamic damage and its repair in vibrio cholerae. | 1983 | 6634957 | |
| nature of interaction of different wild type vibrio cholerae with intestinal mucosa. | scanning electron microscopy (sem) was used in this study to investigate the mechanism of association of cholera vibrios with the intestinal mucosa. for this purpose, the interactions of three virulent, wild type strains (ca401, p, and 3083) of vibrio cholerae with intestinal segments of infant mice were compared. the results of previous studies by different investigators using the three strains separately and in different assay systems had led to differing conclusions concerning the mechanisms ... | 1983 | 6635553 |
| improved method, using staphylococcal beta-hemolysin, for detection of hemolysin(s) produced by vibrio cholerae biotype el tor. | a tube test using brain heart infusion broth and staphylococcal b-lysin (hibl) was devised to improve the detection of vibrio cholerae el tor hemolysin. fifty six (100%) strains of v. cholerae serotypes ogawa (28) and inaba (28) were positive by the hemolysin test whereas 4 inaba and 2 ogawa were positive by a standard tube test using heart infusion broth (hib) and 20 ogawa and 18 inaba were positive by another tube test using hib containing glycerol (hibg). seven classical v. cholerae strains t ... | 1983 | 6635756 |
| enteric fever in patients admitted to a diarrhoeal disease hospital in bangladesh. | the hospital records of 62 patients with blood culture-proven enteric fever admitted to the dacca hospital of the international centre for diarrhoeal disease research, bangladesh, over a one-year period were reviewed. older children and young adults had the highest age-specific rates of disease. the clinical, epidemiological and laboratory features of patients with enteric fever were compared with similar information from patients in hospital for cholera and shigellosis. patients with enteric fe ... | 1983 | 6636284 |
| effects of nutrient deprivation on vibrio cholerae. | environmental and clinical strains of vibrio cholerae were exposed to nutrient-free artificial seawater and filtered natural seawater microcosms for selected time intervals and examined for changes in cell morphology and number. cells observed by transmission electron and epifluorescence microscopy were found to undergo gross alterations in cell morphology with time of exposure. the vibroid cells decreased in volume by 85% and developed into small coccoid forms surrounded by remnant cell walls. ... | 1983 | 6639037 |
| binding and phagocytosis of sialidase-treated rat erythrocytes by a mechanism independent of opsonins. | rat peritoneal macrophages bind and phagocytoze homologous sialidase-treated erythrocytes at a rate which is dependent on the amount of sialic acid that has been removed from the cells. increased binding of erythrocytes is observed after the removal of 10-20% of membrane sialic acid, while for phagocytosis at least 30-40% of this substance must be removed. with vibrio cholerae sialidase only a partial (80%) hydrolysis of rat erythrocyte sialic acid is possible, whereas arthrobacter ureafaciens s ... | 1983 | 6642429 |
| production of cholera toxin-like toxin by vibrio mimicus and non-o1 vibrio cholerae: batch culture conditions for optimum yields and isolation of hypertoxigenic lincomycin-resistant mutants. | vibrio mimicus 61892, isolated in 1977 from a case of watery diarrhea in bangladesh, produces an enterotoxin which possesses activity in y-1 mouse adrenal cells and in rabbit ileal loops which is identical to the prototype cholera toxin (ct) produced by vibrio cholerae 569b. the neutralization of the adrenal cell activity of 61892 toxin and 569b ct by homologous and heterologous antisera generates parallel titration curves which show complete neutralization in all cases. paired titrations in the ... | 1983 | 6642640 |
| differential complement activation and susceptibility to human serum bactericidal action by vibrio species. | the ability of vibrio vulnificus to resist human serum bactericidal action and to activate human complement was compared with similar cultures of vibrio cholerae and vibrio parahaemolyticus. both v. vulnificus and v. parahaemolyticus had similar survival rates in sera and were much more resistant to killing than was v. cholerae. in contrast, v. vulnificus activated significantly less serum complement than did v. cholerae and v. parahaemolyticus. the relative ability of v. vulnificus to survive i ... | 1983 | 6642665 |
| vibrio cholerae expresses iron-regulated outer membrane proteins in vivo. | a comparison was made, using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, of the outer membrane proteins of four strains of vibrio cholerae grown in vivo in infant rabbits and in vitro in low-iron and iron-supplemented defined media. in vivo-grown v. cholerae expressed novel outer membrane-associated proteins which, in part, were similar to those observed on v. cholerae grown in vitro under conditions of iron deprivation. | 1983 | 6642675 |
| non 0-group 1 vibrio cholerae infection in a desert tortoise (gopherus berlanderi). | 1983 | 6644937 | |
| cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. | nucleotide sequence and deletion analysis have been used to identify the regulatory and coding sequences comprising the cholera toxin operon (ctx). incorporation of defined in vitro-generated ctx deletion mutations into vibrio cholerae by in vivo genetic recombination produced strains which have practical value in cholera vaccine development. | 1983 | 6646234 |
| studies on bacteriocin production by nag-strains of vibrio cholerae as a possible epidemiological marker. | 201 "non-agglutinable" - i.e. serogroup o:1-negative - vibrio cholerae strains were tested for bacteriocin production using 14 of the abbott-shannon standard set of colicin-sensitive strains as indicators. 41.2% of the strains proved to be bacteriocin producers. 29 out of 67 (43%) vibriocin-positive strains originating from one large geographical region gave the same reaction pattern with the applied indicator strains. the possible significance of these vibriocins for epidemiological studies is ... | 1983 | 6649979 |
| vibrio cholerae (non-o1) isolated from california coastal waters. | nineteen strains of vibrio cholerae non-o1 were isolated from five separate marine sites along the santa cruz county coast. this environmental study was initiated after a human case of non-o1 cholera-like diarrhea was acquired endemically. | 1983 | 6651299 |
| a numerical taxonomic study of species of vibrio isolated from the aquatic environment and birds in kent, england. | a numerical taxonomic study has been carried out to confirm the identity of strains of the family vibrionaceae isolated during an ecological study. a total of 237 strains were studied including 148 from the aquatic environment, 6 from estuarine birds, 1 from sheep faeces, and 61 control cultures. duplicates of 21 of the strains were randomly selected and included to estimate test and operator error. taxonomic resemblance was estimated on the basis of 148 characters using euclidean distance. the ... | 1983 | 6654766 |
| absence of nucleotide sequence homology between genes for vibrio cholerae toxin and vibrio fluvalis. | 1983 | 6655296 | |
| phage types of vibrio cholerae o1 biotype el tor isolated from patients and family contacts in bangladesh: epidemiologic implications. | the epidemiology of vibrio cholerae o1 el tor in rural bangladesh was examined with a new phage-typing system for characterization of individual strains. during a two-year period, 537 strains were typed with a set of standard and experimental phages. four major and many minor patterns were identified, some associated with discrete outbreaks of disease and others persisting for the entire period. one outbreak-related phage type was associated with a c plasmid bearing multiple drug resistance. the ... | 1983 | 6655301 |
| bacterial chemotaxis to effluent from a rum distillery in tropical near-shore coastal waters. | pseudomonas aeruginosa and vibrio cholerae showed a strong positive chemotactic response towards rum distillery wastewaters (mostos) and a high oxygen uptake rate in the presence of this complex substrate. rum slops stimulated only motility in aeromonas hydrophila and escherichia coli. the a. hydrophila and e. coli isolates were unable to oxidize mostos significantly. | 1983 | 6660880 |
| seasonality of classical and el tor cholera in dhaka, bangladesh: 17-year trends. | data on the cholera patients admitted monthly to the icddr, b, hospital in dhaka (bangladesh) from 1964 through 1980 have been studied and analysed. although vibrio cholerae variant el tor did not enter bangladesh until 1963, it has completely displaced classical cholera since 1973. there have also been changes in seasonality, which are discussed. | 1983 | 6665839 |
| antimicrobial properties of some plant extracts against bacteria. | plant extracts obtained from feronia limonia (leaves) xanthium strumarium (flowering twigs) and glossocardia bosvellia (leaves) were tested for their antimicrobial properties against certain bacterial species. feronia leaf extract was ineffective on bacillus pumilus and x. campestris, vibrio cholerae was found quite sensitive to this extract. the extract of x. strumarium showed an abnormality with v. cholerae, where the inhibition exceeded the control with established antibiotics. similarly, g. ... | 1983 | 6667916 |
| [behavior of el tor cholera vibrio in various environments, especially in foodstuffs and live shellfish]. | 1983 | 6668347 | |
| effect of berberine chloride binding on the structure of cholera phage phi 2 dna. | 1983 | 6671684 | |
| immunogenicity of soluble haemagglutinin-lipopolysaccharide complex of classical vibrio cholerae. | soluble haemagglutinin-lipopolysaccharide complexes were found to be good antigens since it elicited high levels of the antibodies in the intestine especially of the iga class. these specific antibodies sustained for a long period of time at the significantly high levels (longer than 6 months). the enteric memory primed by the antigens in the intestinal tract were longer than 3 months. pools of intestinal fluids obtained from mice immunized with single dose of sh-lps at 1 week, 1 month and 3 mon ... | 1983 | 6673133 |
| [diagnosis of vibrio cholerae in the laboratory]. | the authors describe a new specific and immunologic process for v. cholerae isolation. specific anti-v. cholerae antibodies (specific igg anti-fraction ch 1 + 2) are sticked on magnetic beads. these beads are added to choleric stool. v. cholerae germs stick on the beads. these particles are drawn out of the mixture with the help of a magnet and laid on a mueller-hinton agar plate. incubation is carried out for 18 hours. one drop of toluen is then added on the beads. wells are sinked in the agar ... | 1983 | 6673858 |
| modulation of antibody responses to vibrio cholerae in mice by adoptive transfer of peyer's patch lymphocytes from orally immunised donors. | peyer's patch lymphocytes from mice orally primed with v. cholerae injected into recipient mice together with v. cholerae led to a considerable suppression of igm antibody-forming cells in the spleen by comparison with control mice receiving the same schedule but with peyer's patch lymphocytes from unprimed mice. the effect on the splenic iga responses of recipients was variable. incomplete separation of the peyer's patch cells showed that both t and b cell enriched fractions were active in this ... | 1983 | 6675649 |
| study of vibrio cholerae non-01 (non agglutinable vibrios). | 1983 | 6677405 | |
| use of cholera toxoid in an enzyme-linked immunosorbent assay for antitoxin. | a glutaraldehyde-inactivated toxoid was evaluated as a coating antigen in an elisa for cholera antitoxin. a reference panel of 36 human sera with antitoxin levels determined by several other assay systems and 58 sera from an outbreak of illness due to vibrio cholerae were studied. toxoid compared favorably with two purified cholera toxins, and the microtiter assay using all three antigens was effective in detecting antibody in known convalescing cholera patients. | 1983 | 6680095 |
| the curing of alpha-phage by superinfection with vca-1 phage in vibrio cholerae. | 1983 | 6680121 | |
| a rapid test for the identification of vibrio cholerae in stools. | a rapid test to identify vibrio cholerae in stools has been developed. the test depends on the ability of the vibrios to multiply in a specially designed medium in the presence of other intestinal bacteria and to agglutinate against specific antisera directly. the culture medium consisted of 2 parts: agar and broth. aseptic condition was not required. a 0.5 ml amount of a diluted stool suspension was added to an equal volume of molten agar in freeze drying glass ampules and left to set whil ... | 1983 | 6680124 |
| effect of different salts on the infectivity of cholera bacteriophage phi 149 for vibrio cholerae 154. | 1983 | 6680125 | |
| anionic forms of brain arylsulfatase b: evidence for a phosphorylated form in man and monkey. | arylsulfatase a, b and an anionic form of b were separated by deae-cellulose column chromatography from the brains of man, monkey, rabbit, rat and chicken. the relative proportion of brain arylsulfatases differed from one species to the other. the anionic form of arylsulfatase b was a minor component as compared to arylsulfatase a or b in human and monkey brains while it was a major component in rat and chicken brains. anionic arylsulfatase b was found in fetal human brains and in newborn monkey ... | 1983 | 6680691 |
| induction of a novel morphological response in chinese hamster ovary cells by pertussis toxin. | exposing chinese hamster ovary cells in culture to pertussis toxin resulted in a novel clustered growth pattern. the specificity of the response for pertussis toxin was shown by neutralization of the activity with specific anti-toxin antibody, heat lability (80 degrees c for 15 min), and absence of such activity by culture media from nontoxigenic bordetella species. although a lag of at least 16 h was required before clustered growth was seen, exposure to the toxin for as little as 10 min result ... | 1983 | 6682833 |
| genetic mapping of vibrio cholerae enterotoxin structural genes. | the structural genes which constitute the cholera toxin operon, ctxab, were genetically mapped in the vibrio cholerae el tor strain rv79. this strain of v. cholerae contains two copies of the ctx operon located on a 7-kilobase-pair tandemly duplicated region. we began by isolating a vibriophage vca1 insertion mutation in one of the two ctxa genes located in this region. the mutant carrying this ctxa::vca1 insertion, dc24, was converted to a vca1-facilitated donor by introduction of the conjugal ... | 1984 | 6690422 |
| evaluation in humans of attenuated vibrio cholerae el tor ogawa strain texas star-sr as a live oral vaccine. | texas star-sr, an a- b+ mutant derived by nitrosoguanidine treatment from vibrio cholerae el tor ogawa strain 3083, was fed to 68 volunteers as an oral vaccine in doses of 10(5) to 5 x 10(10) organisms with nahco3. sixteen (24%) vaccinees experienced some loose stools (unrelated to vaccine dose), but in only one did the total stool volume exceed 1.0 liter. the vaccine strain was cultured from duodenal fluid of 35 of 46 (76%) persons who ingested doses of 10(8) organisms or greater. no a+ b+ toxi ... | 1984 | 6693169 |
| sialosylglobotetraosylceramide: a marker for amyotropic lateral sclerosis. | gangliosides of healthy and pathologic muscles (amyotropic lateral sclerosis and facio-scapulo-humeral muscular dystrophy) were studied. total ganglioside content of the affected muscles was approximately 2 fold higher than the unaffected muscles. our results showed that als muscle contained a ganglioside which was absent in the unaffected and fsh muscular dystrophic muscles. based on the results of hydrolysis with vibrio cholerae neuraminidase and subsequent reactivity of the asialo derivative ... | 1984 | 6696769 |
| chemical and histochemical studies of normal and diseased human gastrointestinal tract. i. a comparison between histologically normal colon, colonic tumours, ulcerative colitis and diverticular disease of the colon. | chemical and histochemical methods were used to compare the epithelial glycoproteins from formalin-fixed surgical specimens of normal human large intestine, colonic tumours, ulcerative colitis and diverticular disease. all the epithelial glycoproteins contained fucose, galactose, glucosamine, galactosamine and, in addition, sialic acids both with and without o-acyl substituents in the side chain and/or at position c4. the glycoproteins of the normal ascending and descending colons differed signi ... | 1984 | 6698804 |
| [experimental justification for combined subcutaneous immunization against cholera and typhoid fever]. | the article presents materials on experiments substantiating the method for the subcutaneous administration of chemical adsorbed typhoid vaccine by means of a jet-injector and the possibility of using this preparation in combination with cholerogen toxoid. | 1984 | 6702364 |
| vibriobactin, a siderophore from vibrio cholerae. | a novel siderophore (microbial iron transport compound) has been isolated from low iron cultures of vibrio cholerae. belonging to the catecholamide family of chelators, it has been shown to contain three residues of 2,3-dihydroxybenzoic acid and two residues of threonine. both threonine moieties are present in the form of oxazoline rings. furthermore, the polyamine backbone of the molecule was proved to be not spermidine, but the rare n-(3-aminopropyl)-1,3-diaminopropane, norspermidine. the stru ... | 1984 | 6706943 |
| sea water and vibrio cholerae. | 1984 | 6708907 | |
| [effect of gamma radiation on the immunobiological and immunochemical properties of cholera exotoxin. i. change in the biological activity of nonpurified cholera exotoxin as affected by ionizing radiation]. | crude cholera exotoxin (filtrate toxin) was irradiated with increasing doses of gamma radiation. a significant drop in enterotoxicity, in the activity of the permeation factor and a decrease in toxicity were shown to occur as radiation doses increased. radiation doses of 50-70 kgy were found to completely inactivate enterotoxicity in liquid toxic preparations. a higher radioresistance of dried preparations in comparison with liquid ones was registered: inactivation occurred at 150-200 kgy. diffe ... | 1984 | 6711194 |
| [reactogenicity and immunological effectiveness of a chemical sorbed typhoid vaccine in combination with choleragen-anatoxin administered subcutaneously by jet injector]. | the article presents immunological data substantiating the combined immunization of humans with the mixture of choleragen toxoid and chemical adsorbed typhoid vaccine, introduced subcutaneously by means of a jet injector. | 1984 | 6711201 |
| cytolytic activity and virulence of vibrio damsela. | a correlation was observed between the ability of 19 isolates of vibrio damsela, a halophilic bacterium recently recognized as a human pathogen, to cause disease in mice and to produce large amounts of a cytolytic toxin in vitro. the yield of toxin in the culture supernatant fluids was optimal during the mid- and late-logarithmic phases of growth in medium containing 0.5% na+ ion, was stable during the stationary growth phase, and was significantly reduced in culture medium containing greater th ... | 1984 | 6715036 |
| compared colonizing and immunizing efficiency of toxinogenic (a+ b+) vibrio cholerae and an a- b+ mutant (texas star-sr) studied in adult rabbits. | four strains of vibrio cholerae o1 were compared for their ability to colonize and immunize adult rabbit intestine. three were virulent, toxinogenic (a+ b+) isolates, and one, an a- b+ mutant (texas star-sr), was derived by mutagenesis with nitrosoguanidine. when given orally to nonimmune rabbits, virulent strains colonized the small bowel with similar efficiency, whereas texas star-sr colonized poorly. rabbits fed less than 50 cfu of an a+ b+ strain developed marked serotype-specific resistance ... | 1984 | 6715038 |
| evaluation of the human immune response to outer membrane proteins of vibrio cholerae. | the immune response of 114 volunteers with diarrhea after experimental challenge with four strains of vibrio cholerae o1 was characterized in a microtiter enzyme-linked immunosorbent assay antibody detection system by using a partially purified outer membrane preparation (omp) from these strains as an antigen. analysis of paired sera from 29 persons with noncholera diarrhea (negative control population), demonstrated that a rise in net optical density greater than 0.10 was significant. a total o ... | 1984 | 6715044 |
| present status of cholera vaccines. | 1984 | 6724113 | |
| non-01 vibrio cholerae gastroenteritis in northern california. | 1984 | 6730494 | |
| a common source foodborne outbreak of cholera in singapore. | an epidemiological investigation of an outbreak of vibrio cholerae 01, biotype el tor, serotype ogawa, phage type 1, confined to a group of foreign construction workers in singapore is described. a total of 22 workers were confirmed to have cholera and another 15 had asymptomatic vibrio cholerae 01 infection between 3 november and 11 november 1982. the source of infection was traced to contaminated seafood prepared at the construction site canteen where two food handlers were found to be infecte ... | 1984 | 6735567 |
| rapid screening of fecal samples for vibrio cholerae by a coagglutination technique. | a coagglutination procedure for detecting vibrio cholerae was applied directly to 125 watery fecal samples received in the laboratory for bacteriological culture: many of these were from suspected cases of cholera. of 47 bacteriologically proved cases of cholera, 44 (93.6%) gave positive results by the coagglutination method. there was a good correlation between the coagglutination method, dark-field microscopy, and culture. | 1984 | 6736229 |
| mucosal antitoxic and antibacterial immunity after cholera disease and after immunization with a combined b subunit-whole cell vaccine. | mucosal and systemic immune responses to a new oral cholera vaccine, consisting of the b subunit plus killed vibrios, were studied in bangladeshi volunteers and compared with those to clinical cholera. a single peroral dose of vaccine induced a local iga antitoxin response in intestinal-lavage fluid of seven of eight vaccinees; the response closely mimicked that of patients convalescing from cholera, and evidence of the induction of local immunologic memory was found as well. two peroral doses w ... | 1984 | 6736680 |
| ecology of non-o 1 vibrio cholerae in toyama prefecture. | the ecology of non-o 1 vibrio cholerae and vibrio mimicus as causes of cholera-like diarrhea or seafood-associated gastroenteritis has been investigated in toyama prefecture since 1980. the relationship between biological or serological characteristics of the isolates and their enteropathogenicity is discussed. overall isolation rates from river water, sea water, and fish were 24.0, 59.5, and 33.7%, respectively, the isolation frequency being, in general, extremely high in the summer season, alt ... | 1984 | 6738382 |
| characterization and distribution of the cell-bound haemagglutinins produced by vibrio cholerae. | the two biotypes of vibrio cholerae were found to produce two distinct cell-bound haemagglutinins (has). el tor vibrios, most of their nonmotile mutants and nonpathogenic el tor strains produced a mannose-sensitive cell-bound ha constitutively under all growth conditions examined. some classical vibrios, their nonmotile mutants and antigenically rough mutants of classical strains produced a fucose-sensitive cell-bound ha continually. other classical vibrios produced neither cell-bound ha nor a f ... | 1984 | 6740382 |
| [quantitative parameters of the length of time of vibrio eltor survival on household objects]. | on household articles contaminated with protein-containing excrements (as in the stool of cholera patients) v. eltor survive 4.6 times longer than on those contaminated with excrements without protein (as in the stool of vibriocarriers ). this is due to a lesser initial dose of vibrios and the absence of protein in the excrements of vibriocarriers . these differences in the survival time of v. eltor on household articles suggest that objects contaminated with the excrements of cholera patients a ... | 1984 | 6741357 |
| [resistance to antibiotics of vibrio cholerae and its possible prognostic significance]. | control of antibiotic resistance of vibrio cholerae in various areas of the world revealed the characteristic features of the situation and tendencies of antibiotic resistance development in this microbial group. it was shown that though the main part of the el tor vibrio of the 7th cholera pandemia period was highly sensitive to antibiotics, separate strains with stable plasmid antibiotic resistance were isolated. by the end of the 70s the spread of resistant cholera germs was registered. the a ... | 1984 | 6742803 |
| bacteria associated with crabs from cold waters with emphasis on the occurrence of potential human pathogens. | a diverse array of bacterial species, including several potential human pathogens, was isolated from edible crabs collected in cold waters. crabs collected near kodiak island, alaska, contained higher levels of bacteria than crabs collected away from regions of human habitation. the bacteria associated with the crabs collected near kodiak included yersinia enterocolitica, klebsiella pneumoniae, and coagulase-negative staphylococcus species; the pathogenicity of these isolates was demonstrated in ... | 1984 | 6742824 |
| seasonal variations of vibrio cholerae (non-o1) isolated from california coastal waters. | this report compares recovery of non-o1 vibrio cholerae strains from seven california coastal sites during the winter and summer of 1983. a total of 41 identified and 27 presumptive nn-o1 v. cholerae strains were recovered from six of seven coastal sites in the summer. a 5-to 56-fold increase in the numbers of organisms isolated from different sites occurred in the summer months, when water temperatures were 1.9 to 5.1 degrees c higher. at the three sites where the highest levels of non-o1 v. ch ... | 1984 | 6742842 |
| effect of carbohydrates on adherence of vibrio cholerae to rabbit intestine & haemagglutination of sheep erythrocytes. | 1984 | 6746031 | |
| elucidation of the phagocytosis mechanism with the aid of luminous bacteria. | phagocytosis of the luminous bacterium vibrio cholerae var. albensis caused a similar decrease both in viable count and in the in-vivo luminescence. these effects of polymorphonuclear neutrophil leukocytes (pmn) were oxygen-dependent processes. exposure of pmn to oxygen caused a prompt decrease in the luminescence of bacteria that had been ingested in anaerobic conditions. cell-free supernates from active pmn suspensions caused a decrease in luminescence and as much as 10% of the killing could b ... | 1984 | 6748042 |
| [compatibility groups of resistance plasmids from gram negative bacteria isolated in algeria ]. | the plasmids characterized in algeria generally belong to the c and f1 me compatibility groups. with the exception of vibrio cholerae el for the two groups are found in many bacterial species. plasmids belonging to group c are the only stable in vibrio cholerae el tor. the possibility that epidemics of vibrio cholerae el tor carrying c plasmids might be observed, should not be excluded. on the other hand, it is presently impossible to guess what will be the future epidemic plasmid in salmonella ... | 1982 | 6750522 |
| inhibitory effect of indomethacin on skin permeability reactions mediated by heat-labile enterotoxins of salmonella weltevreden, escherichia coli and vibrio cholerae. | indomethacin, a rapid and intense inhibitor of prostaglandin synthesis, was used with a view to find out similarity in secretory mechanism of heat-labile enterotoxins of three diarrhoea producing enteric bacteria viz., salmonella weltevreden, escherichia coli and vibrio cholerae in rabbits. a significant inhibition (90% to 94%) of biological activity of indomethacin pretreated salmonella enterotoxin was evident in indomethacin treated rabbits, whereas the biological activity was found comparativ ... | 1982 | 6750983 |
| [150 years pandemics of asiatic cholera, 1831-1981]. | 1982 | 6750987 | |
| biochemical characterization of extracellular proteases from vibrio cholerae. | isoelectric focusing of culture supernatants from vibrio cholerae el tor 1621 and high protease-producing mutant strain 1621 hip revealed the presence of three different types of extracellular protease. type i protease was the major activity in the wild-type strain and was inhibited by phenylmethylsulfonyl fluoride and by the lima bean trypsin inhibitor. type ii protease was present in the wild type and was the major activity in the high protease-producing mutant. it was resistant to inhibitors ... | 1982 | 6752027 |
| detection of heat-labile-enterotoxin-producing colonies of escherichia coli and vibrio cholerae by solid-phase sandwich radioimmunoassays. | a solid-phase sandwich assay that was able to differentiate heat-labile-enterotoxin-producing colonies of escherichia coli and choleratoxin-producing colonies of vibrio cholerae from nontoxigenic colonies is described. flexible polyvinyl chloride plastic film coated with antibody molecules was allowed to react with partially lysed bacterial colonies in a standard petri dish. the immobilized antigen on the plastic film was then labeled with radioiodinated antibody. autoradiography identified anti ... | 1982 | 6752189 |
| [lipopolysaccharides of vibrio cholerae and enterobacteria]. | 1982 | 6755981 |