Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| highly sensitive determination of dao activity by oxidation of a luminescence reagent. | a highly sensitive method for measuring the activity of the enzyme diamine oxidase (dao) independent of the type of substrate is described. the principle of the assay is to determine the amount hydrogen peroxide generated as a reaction product during oxidation of diamines by dao. psatto, a highly sensitive luminescence reagent, was used to generate a signal depending on the hydrogen peroxide concentration based on the action of horseradish peroxidase. dao is specifically captured from a sample b ... | 2007 | 18025605 |
| [neuronal connections of ocular dominance columns in the cortex of monocularly deprived cats]. | we have investigated the developmental changes of intrahemispheric neuronal connections of the areas 17 & 18 ocular dominance columns in monocularly deprived cats. single cortical columns were microiontophoretically injected with horseradish peroxidase and 3d reconstruction of retrogradely labelled cells' region was done. ocular dominance of injected columns and their coordinates in the visual field map were determined. in area 17 it was shown that for non-deprived eye the connections of columns ... | 2007 | 18030800 |
| novel highly-performing immunosensor-based strategy for ochratoxin a detection in wine samples. | the increasing concern about ochratoxin a (ota) contamination of different food and feedstuffs demands high-performing detection techniques for quality assessment. two indirect competitive enzyme-linked immunosorbent assay (elisa) strategies were investigated for the development of ota electrochemical immunosensors based on different ota immobilisation procedures. immunosensors based on avidin/biotin-ota showed enhanced performance characteristics compared to those based on the adsorption of bov ... | 2008 | 18035531 |
| chemiluminescence flow-through biosensor for glucose with eggshell membrane as enzyme immobilization platform. | in this study, a new chemiluminescence (cl) flow-through biosensor for glucose was developed by immobilizing glucose oxidase (god) and horseradish peroxidase (hrp) on the eggshell membrane with glutaraldehyde as a cross-linker. the cl detection involved enzymatic oxidation of glucose to d-gluconic acid and hydrogen peroxide (h2o2) and then h2o2 oxidizing luminol to produce cl emission in the presence of hrp. the immobilization condition (e.g., immobilization time, god/hrp ratio, glutaraldehyde c ... | 2008 | 18036547 |
| oxidation of myosin by haem proteins generates myosin radicals and protein cross-links. | previous studies have reported that myosin can be modified by oxidative stress and particularly by activated haem proteins. these reactions have been implicated in changes in the properties of this protein in food samples (changes in meat tenderness and palatability), in human physiology (alteration of myocyte function and force generation) and in disease (e.g. cardiomyopathy, chronic heart failure). the oxidant species, mechanisms of reaction and consequences of these reactions are incompletely ... | 2008 | 18039181 |
| novel enzyme/dna/inorganic nanomaterials: a new generation of biocatalysts. | the design, synthesis and properties of a new class of enzyme/dna/inorganic nanobiomaterials are described here. dna has been used to stabilize the enzymes intercalated in the galleries of the inorganic solid, alpha-zr(iv) phosphate (alpha-zr(hpo(4))(2).h(2)o, abbreviated as alpha-zrp). interestingly, the presence of dna improved the activity and stability of the bound enzymes. key studies leading to the current strategy are presented initially, and these are followed by more recent developments ... | 2007 | 18043809 |
| evaluation of enzymatic activity on nanoscale polystyrene-block-polymethylmethacrylate diblock copolymer domains. | understanding structural and functional changes of polymeric surface-bound proteins is extremely important as polymers play an increasingly significant role as arrays and substrates in proteomics applications. we carried out, for the first time, quantitative activity measurements of horseradish peroxidase (hrp) enzymes immobilized selectively on the polystyrene domains of microphase-separated polystyrene-block-polymethylmethacrylate ultrathin films. the specific enzymatic activity of hrp adsorbe ... | 2007 | 18044871 |
| redox state of dipyridamole is a critical determinant for its beneficial antioxidant and antiinflammatory effects. | dipyridamole, a well-known inhibitor of cgmp-dependent phosphodiesterase and the adenosine transporter, reportedly possesses antioxidant properties and attenuates reactive oxygen species (ros) formation in platelet and endothelial cells. the relevance of the redox status of this compound or the mechanism for its redox-dependent effects is unknown. oxidation of dipyridamole by horseradish peroxidase and hydrogen peroxide diminished its fluorescence and attenuated dipyridamole-mediated dpph and fe ... | 2007 | 18049314 |
| effect of ionic liquid on the kinetics of peroxidase catalysis. | the effect of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][bf4]), on the horseradish peroxidase (hrp)-catalyzed oxidation of 2-methoxyphenol (guaiacol) with hydrogen peroxide (h2o2) was investigated. hrp maintains its high activity in the aqueous mixtures containing various concentrations of the ionic liquid and even in 90% (v/v) ionic liquid. in order to minimize the effect of solution viscosity on the kinetic constants of hrp catalysis, the enzymatic rea ... | 2007 | 18051270 |
| protecting peroxidase activity of multilayer enzyme-polyion films using outer catalase layers. | films constructed layer-by-layer on electrodes with architecture {protein/hyaluronic acid (ha)}n containing myoglobin (mb) or horseradish peroxidase (hrp) were protected against protein damage by h2o2 by using outer catalase layers. peroxidase activity for substrate oxidation requires activation by h2o2, but {protein/ha}n films without outer catalase layers are damaged slowly and irreversibly by h2o2. the rate and extent of damage were decreased dramatically by adding outer catalase layers to de ... | 2007 | 18052272 |
| arginine-to-lysine substitutions influence recombinant horseradish peroxidase stability and immobilisation effectiveness. | horseradish peroxidase (hrp) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. often, it is used in immobilised form. with conventional immobilisation techniques, the enzyme adheres in random orientation: the active site may face the solid phase rather than bulk medium, impeding substrate access and leading to sub-optimal catalytic performance. the ability to immobilise hrp in a directional manner, such that the active site would always fa ... | 2007 | 18053254 |
| direct electrochemistry and electrocatalysis of horseradish peroxidase based on clay-chitosan-gold nanoparticle nanocomposite. | gold nanoparticles stabilized by chitosan (aucs) were hybridized with exfoliated clay nanoplates through electrostatic interaction. the resulting clay-chitosan-gold nanoparticle nanocomposite (clay/aucs) was used to modify glassy carbon electrode (gce). hrp, a model peroxidase, was entrapped between the clay/aucs film and another clay layer. uv-vis spectrum suggested hrp retained its native conformation in the modified film. basal plane spacing of clay obtained by x-ray diffraction (xrd) indicat ... | 2008 | 18054482 |
| a bienzyme channeling glucose sensor with a wide concentration range based on co-entrapment of enzymes in sba-15 mesopores. | a novel bienzyme-channeling sensor was constructed by entrapping glucose oxidase (god) and horseradish peroxidase (hrp) in the mesopores of well-ordered hexagonal mesoporous silica structures (sba-15). the sba-15 mesoporous materials accelerated the electron transfer between the entrapped hrp and electrode. both hrp and god retained their catalytic activities in the bienzyme-entrapped sba-15 film. in presence of glucose the enzymatic reaction of god-glucose-dissolved oxygen system generated hydr ... | 2008 | 18054830 |
| an amperometric hydrogen peroxide biosensor based on immobilization of horseradish peroxidase on an electrode modified with magnetic dextran microspheres. | a new kind of magnetic dextran microsphere (mdms) with uniform shape and narrow diameter distribution has been prepared from magnetic iron nanoparticles and dextran. horseradish peroxidase (hrp) was successfully immobilized on the surface of an mdms-modified glassy-carbon electrode (gce), and the immobilized hrp displayed excellent electrocatalytic activity in the reduction of h(2)o(2) in the presence of the mediator hydroquinone (hq). the effects of experimental variables such as the concentrat ... | 2008 | 18060389 |
| monolithic macroporous albumin/chitosan cryogel structure: a new matrix for enzyme immobilization. | a novel monolithic macroporous material was developed by cross-linking hen egg albumin (hea) and chitosan with glutaraldehyde at subzero temperatures. a macroporous cryogel structure allowed efficient mass transport of solutes within the material. in one application, albumin was partially replaced with active enzymes (glucose oxidase and horseradish peroxidase) resulting in the production of macroporous biocatalyst preparations suitable for flow-injection analysis of glucose in the low millimola ... | 2008 | 18066683 |
| organization of connectivity of the rat presubiculum: ii. associational and commissural connections. | the regional, laminar, and longitudinal organization of intrinsic projections in the presubiculum was examined in the rat with the retrograde tracer horseradish peroxidase conjugated to wheat germ agglutinin and the anterograde tracer phaseolus vulgaris-leucoagglutinin. cells of origin of intrinsic projections in the presubiculum were distributed in layers ii and v, with almost none in layers iii and vi. projections from layer ii cells were bilateral and confined to layers ii and v and also to t ... | 2008 | 18067148 |
| improved activity of enzymes in mixed cationic reverse micelles with imidazolium-based surfactants. | this work reports the significant enhancement in performance of interfacially active enzymes, chromobacterium viscosum (cv) lipase and horseradish peroxidase (hrp) in mixed reverse micelles of cetyltrimethylammonium bromide (ctab) and imidazolium-based amphiphiles having varying tail lengths. lipase activity in these mixed systems was always higher than that in the individual cationic reverse micelles of ctab or any imidazolium surfactant, highest being observed in the mixed system of ctab (50 m ... | 2008 | 18067868 |
| encapsulation of quercetin and myricetin in cyclodextrins at acidic ph. | the in vitro formation of quercetin- and myricetin-cyclodextrin inclusion complexes in acidic medium has been characterized using the enzymatic system horseradish peroxidase, which oxidizes those flavonols in the presence of h2o2. the presence of cyclodextrins (cds) in the reaction medium inhibited flavonol oxidation due to the complexation of the flavonol in the hydrophobic cavity of cds. this inhibitory effect depends on the complexation constant kc between flavonol and the cd type used. the k ... | 2008 | 18069793 |
| immunoassay using microfluid filters constructed by deep x-ray lithography. | microfluid filters were fabricated, which possessed 2,100 cylindrical through-bores (psi 40 microm) in 200 microm-thickness polymethylmethacrylate (pmma) sheets (psi 3 mm), by deep x-ray lithography using synchrotron radiation. to evaluate the microfluid filters as a device for an immunoassay, we bound the goat anti-mouse immunoglobulin g (igg) antibody to the surface of the filters, and set the filters between reaction vessels stacked vertically in a microreactor. an enzyme-linked immunosorbent ... | 2007 | 18071245 |
| effectiveness of phenoxyl radicals generated by peroxidase/h2o2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and nadh. | the rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (nadh) cooxidation (i.e., their nonenzymic oxidation by peroxidase/h2o2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. the reactions initiated by different sources of peroxidase (ec 1.11.1.7) [isolates from soybean (glycine max l.) seed coat, maize (zea mays l.) root-cell wall, and commercial horseradish peroxidase] were monitored. native electrophoresis of ... | 2008 | 18071845 |
| matrix metalloproteinase-9 is involved in the development and plasticity of retinotectal projections in rats. | during postnatal development, retinotectal projections undergo a process of misplaced axon elimination, leading to a topographical matching between the retinal surface and the superior colliculus. matrix metalloproteinases (mmps) have been implicated in the development and plasticity of the nervous system. we studied the expression and role of mmps during normal development of retinotectal projections and after monocular enucleation-induced plasticity. | 2007 | 18073506 |
| modification of motoneuron size after partial denervation in neonatal rats. | our previous studies have shown that partial denervation of extensor digitorum longus muscle (edl) in the rat at 3 days of age causes an increase in the activity of the intact motoneurons. the originally phasic pattern of activity of edl became tonic after partial denervation. these modifications of motoneuron activity were associated with the change in the phenotype of the muscle from fast to slow contracting and with a conversion of the muscle fibres from a fast to a slow type. the present stu ... | 2007 | 18075126 |
| regeneration of retinotectal projections after optic tectum removal in adult newts. | when injured, the adult newt possesses the remarkable capability to regenerate tissues and organs with return of function and physiology. one example is the newt eye, in which regeneration can restore normal vision if the retina or lens has been removed. we wanted to examine how the retinotectal projections regenerate after removal of the brain's optic tectum and establish this animal as a model for retinal projection as well as a central nervous system regeneration model. | 2007 | 18079683 |
| antigen heterologous enzyme linked immunosorbent assay for the measurement of estrone-3-glucuronide. | we report a novel antigen heterologous enzyme linked immunosorbent assay for the direct estimation of estrone-3-glucuronide (e1-3-g) in diluted human urine. the differential behavior of antibody towards the labeled and unlabelled analyte in heterologous systems forms the basis of the present assay. antiserum was raised in new zealand white rabbits using estrone-3-glucuronide-bovine serum albumin (e1-3-g-bsa) as immunogen and nandrolone-17-hs (n-17-hs) coupled to horseradish peroxidase (hrp) with ... | 2008 | 18080882 |
| a novel recombinantly produced banana lectin isoform is a valuable tool for glycoproteomics and a potent modulator of the proliferation response in cd3+, cd4+, and cd8+ populations of human pbmcs. | lectins as carbohydrate-binding proteins have been employed in various biological assays for the detection and characterization of glycan structures on glycoproteins, including clinical biomarkers in disease states. a mannose-specific banana lectin (banlec) is unique in its specificity for internal alpha1,3 linkages as well as beta1,3 linkages at the reducing termini. the immunomodulatory potential of natural banlec was recognized by a strong immunoglobulin g4 antibody response and t cell mitoge ... | 2008 | 18083059 |
| nigrostriatal neurons in rat express the glial cell line-derived neurotrophic factor receptor subunit c-ret. | the substantia nigra neurons expressing c-ret, a glial cell line-derived neurotrophic factor (gdnf) receptor intracellular tyrosine kinase subunit, were investigated in rats by using a double labeling method which combined retrograde horseradish peroxidase (hrp) labeling after injection into the striatum with immunohistochemistry to c-ret. it was revealed that the distribution of c-ret-immunoreactive neurons and hrp-labeled nigrostriatal neurons overlapped. numerous double-labeled hrp/c-ret neur ... | 2008 | 18085609 |
| morphological and molecular data argue for the labrum being non-apical, articulated, and the appendage of the intercalary segment in the locust. | our analysis of head segmentation in the locust embryo reveals that the labrum is not apical as often interpreted but constitutes the topologically fused appendicular pair of appendages of the third head metamere. using molecular, immunocytochemical and retrograde axonal staining methods we show that this metamere, the intercalary segment, is innervated by the third brain neuromere-the tritocerebrum. evidence for the appendicular nature of the labrum is firstly, the presence of an engrailed stri ... | 2002 | 18088971 |
| embryonic development of the sensory innervation of the antenna of the grasshopper schistocerca gregaria. | the establishment of the sensory nervous system of the antenna of the grasshopper schistocerca gregaria was examined using immunocytochemical methods and in the light of the appendicular and articulated nature of this structure. the former is demonstrated first by the expression pattern of the segment polarity gene engrailed in the head neuromere innervating the antenna, the deutocerebrum. engrailed expression is present in identified deutocerebral neuroblasts and, as elsewhere in the body, is c ... | 2004 | 18089045 |
| embryonic development of a peripheral nervous system: nerve tract associated cells and pioneer neurons in the antenna of the grasshopper schistocerca gregaria. | the grasshopper antenna is an articulated appendage associated with the deutocerebral segment of the head. in the early embryo, the meristal annuli of the antenna represent segment borders and are also the site of differentiation of pioneer cells which found the dorsal and ventral peripheral nerve tracts to the brain. we report here on another set of cells which appear earlier than the pioneers during development and are later found arrayed along these tracts at the border of epithelium and lume ... | 2007 | 18089112 |
| photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays. | we describe the fabrication of poly(ethylene glycol) diacrylate (peg-da) hydrogel microstructures with a high aspect ratio and the use of hydrogel microstructures containing the enzyme beta-galactosidase (beta-gal) or glucose oxidase (gox)/horseradish peroxidase (hrp) as biosensing components for the simultaneous detection of multiple analytes. the diameters of the hydrogel microstructures were almost the same at the top and at the bottom, indicating that no differential curing occurred through ... | 2007 | 18092467 |
| reliable enzyme-linked immunosorbent assay systems for pathogenic factors of pseudomonas aeruginosa alkaline proteinase, elastase, and exotoxin a: a comparison of methods for labeling detection antibodies with horseradish peroxidase. | sensitive sandwich enzyme-linked immunosorbent assay (elisa) systems for the quantification of 3 pathogenic factors of pseudomonas aeruginosa-alkaline proteinase (aeruginolysin), elastase (pseudolysin ), and exotoxin a-were developed. the maleimide-pyridyl disulfide method was applied for the labeling of rabbit anti-each antigen igg with horseradish peroxidase (hrp) and the conjugates were used as secondary antibodies (detection antibodies) in the elisa systems. the edta, a chelating agent, was ... | 2007 | 18094533 |
| radio-activation of htert promoter in larynx squamous carcinoma cells: an 'indirected-activator' strategy in radio-gene-therapy. | the usefulness of human telomerase reverse transcriptase (htert) gene promoter has been proposed in cancer-targeted gene therapy. however, this promoter may not be strong enough to achieve therapeutic levels of transgene expression. in this study, we tested an 'indirected-activator' strategy that utilizes radiation to increase the activity of the htert gene promoter. we demonstrated that htert may participate in the process of dna repair induced by irradiation. we found that zidovudine (azt, an ... | 2008 | 18097608 |
| projections of striatopallidal structures to the pedunculopontine nucleus of the tegmentum of the midbrain in dogs. | a method based on retrograde axonal transport of horseradish peroxidase was used to study the striatopallidal afferent projections of the pendunculopontine nucleus of the midbrain (ppn) in dogs. the major source of these projections was found to be the pallidum, as both the compact and diffuse zones of this nucleus received projections from all of its structures: the entopeduncular nucleus, the globus pallidus, and the ventral pallidum. the striatal complex, specifically the nucleus accumbens, s ... | 2008 | 18097758 |
| the enzyme-substrate compounds of horseradish peroxidase and peroxides; kinetics of formation and decomposition of the primary and secondary complexes. | 1949 | 18131422 | |
| development and validation of a sensitive enzyme immunoassay for surveillance of cry1ab toxin in bovine blood plasma of cows fed bt-maize (mon810). | the increasing global adoption of genetically modified (gm) plant derivatives in animal feed has provoked a strong demand for an appropriate detection method to evaluate the existence of transgenic protein in animal tissues and animal by-products derived from gm plant fed animals. a highly specific and sensitive sandwich enzyme immunoassay for the surveillance of transgenic cry1ab protein from bt-maize in the blood plasma of cows fed on bt-maize was developed and validated according to the crite ... | 2008 | 18155416 |
| [sample pre-processing for mass spectrometric analysis of glycoprotein structure]. | a novel, simple and fast method based on a quadrupole ion trap (qit) and a time-of-flight mass spectrograph was developed for the glycosylation site and glycan structure analysis of glycoprotein by in-solution digestion and in-gel digestion which did not need to enrich and label the glycopeptide. horseradish peroxidase (hrp) and rnase b were utilised to optimize the conditions of sample pre-processing. the effects of the conditions were investigated separately and the optimal conditions were: he ... | 2007 | 18161309 |
| examination of neurons in wild type and mutants of caenorhabditis elegans using antibodies to horseradish peroxidase. | antibodies to horseradish peroxidase (hrp) recognize 27 of 302 neurons and several non-neuronal cells in adult hermaphrodites of the soil nematode caenorhabditis elegans and can be used to label these cells for cytological analysis in whole animals. the antibodies bind to the anterior members, but not to the posterior members of a set of mechanosensory neurons in wild type animals. binding to one of the posterior mechanosensory neurons (pvm) occurs when this neuron migrates to an abnormal anteri ... | 2007 | 18161589 |
| parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations. | a bifunctional aptamer that includes two aptamer units for cocaine and adenosine 5'-monophosphate (amp) is blocked by a nucleic acid to form a hybrid structure with two duplex regions. the blocked bifunctional aptamer assembly is used as a functional structure for the simultaneous sensing of cocaine or amp. the blocked bifunctional aptamer is dissociated by either of the two analytes, and the readout of the separation of the sensing structure is accomplished by a colorimetric detection, by a rel ... | 2008 | 18161727 |
| a novel sensitive immunoassay method based on the invader technique. | a novel and sensitive immunoassay method has been developed in which the conventional sandwich immunoassay and the highly sensitive dna detection method, the invader method, are combined. the signal amplification function of the latter method has been successfully used to enhance the sensitivity of the sandwich immunoassay. the new assay method may be called the immuno-invader assay. the assay format involves three important steps: (1) a target antigen is captured and flagged with a biotin-conju ... | 2008 | 18162168 |
| integrated microfluidic systems with an immunosensor modified with carbon nanotubes for detection of prostate specific antigen (psa) in human serum samples. | this paper describes the development of an immunosensor coupled to glassy carbon electrode (gce) modified with multiwall carbon nanotubes (mwcnt) (cnt-gce) integrated with microfluidic systems for rapid and sensitive quantification of prostate specific antigen (psa) in human serum samples. mouse monoclonal (5g6) to psa antibodies were immobilized on a rotating disk. psa in the serum sample are allowed to react immunologically with the immobilized anti-tpsa and horseradish peroxidase (hrp) enzyme ... | 2008 | 18162392 |
| oxidations of n-(3-indoleethyl) cyclic aliphatic amines by horseradish peroxidase: the indole ring binds to the enzyme and mediates electron-transfer amine oxidation. | although oxidations of aromatic amines by horseradish peroxidase (hrp) are well-known, typical aliphatic amines are not substrates of hrp. in this study, the reactions of n-benzyl and n-methyl cyclic amines with hrp were found to be slow, but reactions of n-(3-indoleethyl) cyclic amines were 2-3 orders of magnitude faster. analyses of ph-rate profiles revealed a dominant contribution to reaction by the amine-free base forms, the only species found to bind to the enzyme. a metabolic study on a fa ... | 2008 | 18163622 |
| the methodology for labeling the distal cerebrospinal fluid-contacting neurons in rats. | the distal cerebrospinal fluid-contacting neurons (dcsf-cns) are one special type of neurons, whose bodies are in the parenchyma of the brain and processes extend into the ventricle, which suggested that dcsf-cns might play the important roles in neuromodulation and neuroendocrinal regulation. without special tracing method, the dcsf-cns is hard to identify in parenchyma of the brain. thus, up to present, these structure and function are seldom investigated. to explore dcsf-cns, the cholera toxi ... | 2008 | 18179825 |
| measurement of ambient hydroperoxides using an automated hplc system and various factors which affect variations of their concentrations in korea. | an hplc system was automated for the measurement of gaseous hydroperoxides in the ambient air. in this system, samples collected using a glass coil without a long sampling tube are injected automatically into hplc. the analytical system is based on the post-column derivatization, which is a reaction of hydroperoxides with p-hydroxyphenyl acetic acid to produce a fluorescent dimer in the presence of horseradish peroxidase as an enzyme catalyst. the detection limits of hydrogen peroxide (h(2)o(2)) ... | 2008 | 18181026 |
| bradykinin increases blood-tumor barrier permeability by down-regulating the expression levels of zo-1, occludin, and claudin-5 and rearranging actin cytoskeleton. | bradykinin (bk) has been shown to open blood-tumor barrier (btb) selectively and to increase permeability of the btb transiently, but the mechanism is unclear. this study was performed to determine whether bk opens the btb by affecting the tight junction (tj)-associated proteins zonula occluden-1 (zo-1), occludin, and caludin-5 and cytoskeleton protein filamentous actin (f-actin). in rat brain glioma model and btb model in vitro, we find that the protein expression levels of zo-1, occludin, and ... | 2008 | 18183615 |
| ferrous campylobacter jejuni truncated hemoglobin p displays an extremely high reactivity for cyanide - a comparative study. | campylobacter jejuni hosts two hemoglobins (hbs). the camplylobacter jejuni single-domain hb (called cgb) is homologous to the globin domain of flavohemoglobin, and it has been proposed to protect the bacterium against nitrosative stress. the second hb is called ctb (hereafter cj-trhbp), belongs to truncated hb group iii, and has been hypothesized to be involved in o(2) chemistry. here, the kinetics and thermodynamics of cyanide binding to ferric and ferrous cj-trhbp [cj-trhbp(iii) and cj-trhbp( ... | 2008 | 18190529 |
| immobilization of horseradish peroxidase on chitosan/silica sol-gel hybrid membranes for the preparation of hydrogen peroxide biosensor. | a simple and effective strategy for fabrication of hydrogen peroxide (h2o2) biosensor has been developed by entrapping horseradish peroxidase (hrp) in chitosan/silica sol-gel hybrid membranes (cshms) doped with potassium ferricyanide (k3fe(cn)6) and gold nanoparticles (gnps) on platinum electrode surface. the hybrid membranes are prepared by cross-linking chitosan (cs) with 3-aminopropyltriethoxysilane (aptes), while the presence of gnps improved the conductivity of cshms, and the fe(cn)6(3-/4-) ... | 2008 | 18199484 |
| effect of ibuprofen l-lysinate on bilirubin binding to albumin as measured by saturation index and horseradish peroxidase assays. | to demonstrate, in vitro, the influence of increasing ibuprofen (ibu) concentration on bilirubin-albumin (b-a) binding. | 2008 | 18200021 |
| the milk-ejection reflex in the sheep: an anatomical study on the afferent pathway. | the present study is an attempt to reveal the spinal and supraspinal organization of the ascending branch of the milk-ejection reflex in the ewe by means of a tract-tracing technique. for this purpose, injections of horseradish peroxidase (hrp) were performed into the lateral cervical nucleus (lcn) and into the hypothalamic paraventricular nucleus (pvn). peroxidase injections into the lcn revealed retrogradely labelled neurons in the medial part of laminae i-iii of the ipsilateral l3 and l4 spin ... | 2008 | 18205887 |
| inhibition of hyaluronan export reduces collagen degradation in interleukin-1 treated cartilage. | osteoarthrosis is characterized by cartilage erosion, proteolysis of aggrecan and collagen, and disturbed rates of synthesis of aggrecan and hyaluronan by chondrocytes, with hyaluronan over-production being an early reaction. we considered that inhibition of hyaluronan export might prevent subsequent proteoglycan loss and collagen degradation. | 2008 | 18205921 |
| enzymatic removal of phenol and p-chlorophenol in enzyme reactor: horseradish peroxidase immobilized on magnetic beads. | horseradish peroxidase was immobilized on the magnetic poly(glycidylmethacrylate-co-methylmethacrylate) (poly(gma-mma)), via covalent bonding and used for the treatment of phenolic wastewater in continuous systems. for this purposes, horseradish peroxidase (hrp) was covalently immobilized onto magnetic poly(gma-mma) beds using glutaraldehyde (ga) as a coupling agent. the maximum hrp immobilization capacity of the magnetic poly(gma-mma)-ga beads was 3.35 mg g(-1). the immobilized hrp retained 79% ... | 2008 | 18207637 |
| new immunosensor for lactoferrin determination in human milk and several pharmaceutical dairy milk products recommended for the unweaned diet. | thorough research was carried out on lactoferrin immunosensor development. furthermore, two different competitive procedures were used for lactoferrin determination, in which either the antigen (lactoferrin) or the antibody (anti-lactoferrin) was, respectively, conjugated with horseradish peroxidase enzyme using a biotinylation process. the biotinylation of lactoferrin and the subsequently used competition procedure for the immunosensor measurement were to get ready. three different kinds of imm ... | 2008 | 18207689 |
| mechanisms of the ifosfamide-induced inhibition of endocytosis in the rat proximal kidney tubule. | the fanconi syndrome is a common side effect of the chemotherapeutic agent ifosfamide. current evidences suggest that chloroacetaldehyde (caa), one of the main metabolites of ifosfamide activation, contributes to its nephrotoxicity. however, the pathophysiology of caa-induced fanconi syndrome is not fully understood. the present work examined the adverse effects of caa on precision-cut rat renal cortical slices, which allowed studying the toxic effect of caa on proximal endocytosis. we demonstra ... | 2008 | 18214444 |
| quantitative detection of allergenic protein sin a 1 from yellow mustard (sinapis alba l.) seeds using enzyme-linked immunosorbent assay. | allergy to yellow mustard (ym; sinapis alba l.) seed proteins has been reported and is currently seen as a constraint that hampers expansion of ym protein utilization. the most predominant allergenic protein of ym seed has been recognized as sin a 1. in this study, sin a 1 was purified ( s. alba var. andante), rabbit polyclonal antibodies (pab) specific to sin a 1 were generated, and a sandwich enzyme-linked immunosorbent assay (s-elisa) was developed to detect and quantify sin a 1 from ym. the ... | 2008 | 18217709 |
| isthmotectal axons maintain normal arbor size but fail to support normal branch numbers in dark-reared xenopus laevis. | developing binocular projections to the xenopus tectum require visual input in order to establish matching topographic maps. in dark-reared xenopus, the ipsilateral eye's map, relayed via the retino-tecto-isthmotectal pathway, fails initially to acquire normal rostrocaudal order. moreover, with extended time in the dark, the ipsilateral map becomes progressively less well organized. this phenomenon showed that without binocular cues, the isthmotectal axons are unable to locate proper sites for t ... | 2008 | 18219666 |
| ultrasensitive electrochemical immunosensor for clinical immunoassay using thionine-doped magnetic gold nanospheres as labels and horseradish peroxidase as enhancer. | a new signal amplification strategy based on thionine (th)-doped magnetic gold nanospheres as labels and horseradish peroxidase (hrp) as enhancer holds promise to improve the sensitivity and detection limit of the immunoassay for carcinoembryonic antigen (cea), as a model protein. this immunoassay system was fabricated on a carbon fiber microelectrode (cfme) covered with a well-ordered anti-cea/protein a/nanogold architecture. the reverse micelle method was initially used for the preparation of ... | 2008 | 18220412 |
| the pathways connecting the hippocampal formation, the thalamic reuniens nucleus and the thalamic reticular nucleus in the rat. | most dorsal thalamic nuclei send axons to specific areas of the neocortex and to specific sectors of the thalamic reticular nucleus; the neocortex then sends reciprocal connections back to the same thalamic nucleus, directly as well indirectly through a relay in the thalamic reticular nucleus. this can be regarded as a 'canonical' circuit of the sensory thalamus. for the pathways that link the thalamus and the hippocampal formation, only a few comparable connections have been described. the reun ... | 2008 | 18221482 |
| immunoblotting and immunodetection. | this unit provides protocols for immunoblotting, which is used to identify specific protein sequences separated by electrophoresis and transferred to an appropriate membrane and recognized by a polyclonal or monoclonal antibody. after the proteins are separated on a gel, they are transferred to a membrane by electroblotting or a semidry transfer system. proteins on the membrane can be visualized with the reversible stain ponceau s to assess the completeness of transfer. then the blot is analyzed ... | 2001 | 18228374 |
| measuring retrograde transport to the trans-golgi network. | the recently described retrograde transport route is a highly selective pathway that allows some internalized molecules to reach the trans-golgi network from early/recycling endosomes, bypassing the recycling route to the plasma membrane and the late endocytic pathway. the non-toxic receptor-binding b-subunit of bacterial shiga toxin has played an important role in the discovery and molecular dissection of membrane trafficking at the early/recycling endosomes-tgn interface. this unit describes s ... | 2006 | 18228477 |
| the influence of carbohydrate structures present in common allergen sources on specific ige results. | cross-reactive carbohydrate determinants (ccd) are well known interferants in specific ige assays (sige). glyco-epitopes are not restricted to ccd and extracts used to prepare in vitro tests contain many other glycoproteins able to bind glycan-specific ige. the overall amounts of ige-bindable glycan structures in allergen sources are unknown. | 2007 | 18236996 |
| suicide-peroxide inactivation of microperoxidase-11: a kinetic study. | the kinetics of microperoxidase-11 (mp-11) in the oxidation reaction of guaiacol (ah) by hydrogen peroxide was studied, taking into account the inactivation of enzyme during reaction by its suicide substrate, h2o2. concentrations of substrates were so selected that: 1) the reaction was first-order in relation to benign substrate, ah and 2) high ratio of suicide substrate to the benign substrate, [h2o2] > [ah]. validation and reliability of the obtained kinetic equations were evaluated in various ... | 2007 | 18237019 |
| peroxidase-mediated formation of the fungal polyphenol 3,14'-bihispidinyl. | medicinal fungi, phellinus linteus and inonotus xeranticus, produce a cluster of yellow pigment in their fermentation broth that acts as an important element of biological activity. the pigment is composed of diverse polyphenols with a styrylpyrone moiety, mainly hispidin and its dimers, 3,14'-bihispidinyl, hypholomine b, and 1,1- distrylpyrylethan. although dimeric hispidins were proposed to be biosynthesized from two molecules of monomer via oxidative coupling by ligninolytic enzymes, laccase ... | 2008 | 18239425 |
| non-covalent surface engineering of an alloplastic polymeric bone graft material for controlled protein release. | alloplastic materials, derived from poly(methylmethacrylate), such bioplant-htr, are a promising alternative to autologous bone in implant-dentistry and maxillofacial reconstruction. the clinical utility and outcomes using alloplasts such as htr can be enhanced through the incorporation and release of proteins and growth factors. a simple, water-based process to surface engineer alloplast material to bear proteins has been developed. in this non-covalent process, the protein of choice is formula ... | 2008 | 18241948 |
| capillary electrophoresis enzyme immunoassay for alpha-fetoprotein and thyroxine in human serum with electrochemical detection. | a novel ce-based enzyme immunoassay (ce-eia) method was developed in o-aminophenol (oap)-h(2)o(2)-horseradish peroxidase (hrp) system and applied to benign liver disease and hyperthyroidism research in the clinical practical field. in the presented method, after the enzyme immunoreaction, the hrp-labeled antibody or hrp-labeled antigen catalyzed the enzyme substrate oap and h(2)o(2). the product of the enzymatic catalysis reaction 2-aminophenoxazine-3-one (ap) was determined using electrochemica ... | 2008 | 18246582 |
| multiplex fluorescent in situ hybridization in zebrafish embryos using tyramide signal amplification. | one of the strengths of the zebrafish is the ease with which in situ hybridization can be performed to determine spatial and temporal patterns of gene expression in whole embryos. thus far, colorimetric detection methods are mainly used for these analyses. here we describe a fluorescent in situ hybridization (fish) protocol for whole-mount zebrafish embryos using tyramide signal amplification (tsa). an optimal set of reagents was identified that allows for simultaneous localization of gene expre ... | 2005 | 18248170 |
| 3,3'-diaminobenzidine (dab)-h2o2-hrp voltammetric enzyme-linked immunoassay for the detection of carcionembryonic antigen. | a new voltammetric enzyme-linked immunoassay system of 3,3'-diaminobenzidine (dab)-h2o2-horseradish peroxidase (hrp) has been presented and used for the sensitive detection of carcinoembryonic antigen (cea) in human serum. in this proposed procedure, dab was firstly used as the electroactive substrate in the hrp catalyzed oxidation reaction in the present of h2o2. the generated product produced a sensitive second-order derivative linear sweep voltammetric peak at potential of -0.62 v (vs. sce) i ... | 2008 | 18248855 |
| gaba- and glycine-like immunoreactivity in axonal endings presynaptic to the vibrissa afferents in the cat trigeminal interpolar nucleus. | the goal of this study was to analyze the synaptic interaction of primary afferents with gaba- and/or glycine-immunopositive presynaptic endings in the cat trigeminal interpolar nucleus (vi). fast adapting vibrissa afferents were labeled by intra-axonal injections of horseradish peroxidase. postembedding immunogold labeling on serially cut ultrathin sections and quantitative ultrastructural analysis of the labeled boutons and their presynaptic endings (p-endings) in the vi were performed. the ma ... | 2008 | 18248903 |
| peroxidase-mediated oxidative cross-linking and its potential to modify mechanical properties in water-soluble polysaccharide extracts and cereal grain residues. | analysis of wheat bran and spent grain shows that concentrations of ferulate and diferulates offer considerable scope to modify the cross-linking of feruloylated polysaccharides and hence the mechanical properties of these residues. in solution ferulic acid (fa) can be readily polymerized by horseradish peroxidase, but when esterified to a polysaccharide, the profile of diferulates becomes restricted. this situation also exists in muro and suggests structural constraints may limit the availabili ... | 2008 | 18251503 |
| adenovirus-mediated retrograde transfer of neurotrophin-3 gene enhances survival of anterior horn neurons of twy/twy mice with chronic mechanical compression of the spinal cord. | chronic mechanical compression of the spinal cord causes neural tissue damage, including loss of anterior horn cells around the level of injury. exogenous delivery of neurotrophins to neuronal cells could provide neuroprotection to a spinal cord subjected to mechanical injury. we investigated the efficacy of retrograde gene delivery of adenoviral vector (adv) carrying neurotrophin-3 (nt-3) gene into twy (twy/twy) mouse spinal cord anterior horn neurons with chronic and progressive mechanical com ... | 2008 | 18253945 |
| sequential affinity purification of peroxidase tagged bispecific anti-sars-cov antibodies on phenylboronic acid agarose. | hybrid hybridomas (quadromas) are derived by fusing at least two hybridomas, each producing a different antibody of predefined specificity. the resulting cell secretes not only the immunoglobulins of both parents but also hybrid molecules manifesting the binding characteristics of the individual fusion partners. purification of the desired bispecific immunoprobe with high specific activity from a mixture of bispecific and monospecific monoclonal antibodies requires special strategies. using a du ... | 2008 | 18258500 |
| asymmetric biomimetic oxidations of phenols: the mechanism of the diastereo- and enantioselective synthesis of thomasidioic acid. | enantiopure chiral amidic derivatives of sinapic acid were oxidised with hydrogen peroxide using horseradish peroxidase (hrp) as the catalyst to give the aryltetraline dilignol thomasidioic acid. trans-diastereoselectivity and enantioselectivity in the formation of thomasidioic acid was observed. computational methods show that the enantioselectivity is controlled by the beta-beta oxidative coupling step, while the diastereoselectivity is controlled by the stability of the reactive conformation ... | 2008 | 18259136 |
| a sensitive resonance scattering spectral assay for the determination of trace h2o2 based on the hrp catalytic reaction and nanogold aggregation. | gold nanoparticles in size of 15 nm exhibit a resonance scattering (rs) peak at 580 nm, in ph 6.4 citrate buffer solutions. horseradish peroxidase (hrp) strongly catalyzed the h2o2 oxidation of o-phenylenediamine to form an intergradation of cyclohexa-3, 5-diene-1, 2-diylidenediamine and the product of 2, 3-diaminophenazin. both cause gold nanoparticles aggregations to enhance the rs intensity at 580 nm greatly. under the optimal conditions, the concentration of h2o2 (c) in the range of 0.08-2.2 ... | 2008 | 18259844 |
| subcellular location of horseradish peroxidase in horseradish leaves treated with la(iii), ce(iii) and tb(iii). | the agricultural application of rare-earth elements (rees) would promote rees inevitably to enter in the environment and then to threaten the environmental safety and human health. therefore, the distribution of the rees ion, (141)ce(iii) and effects of la(iii), ce(iii) and tb(iii) on the distribution of horseradish peroxidase (hrp) in horseradish mesophyll cells were investigated with electron microscopic radioautography and transmission electron microscopic cytochemistry. it was found for the ... | 2008 | 18261795 |
| conjugation of enzymes to antibodies. | conjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (hrpo), urease, or alkaline phosphatase] and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site of the antibody nor the active site of the enzyme is functionally altered. this unit describes procedures for cross-linking hrpo, urease or alkaline phosphatase to immunoaffinity-purified monoclonal or polyclonal antibo ... | 2001 | 18265067 |
| lectin analysis of proteins blotted onto filters. | lectins are proteins that bind with great specificity to certain carbohydrate structures. plant lectins are widely used for investigations of carbohydrate structure and for fractionation and purification of individual oligosaccharides and glycopeptides. this unit describes the use of lectins as sensitive indicators for the presence of certain carbohydrate structures linked to proteins blotted onto filters. a tagged lectin is incubated with a blot containing the target protein and binding of the ... | 2001 | 18265163 |
| labeling and colorimetric detection of nonisotopic probes. | although a number of different nonisotopic labels have been described in the literature, biotin and digoxigenin are used most frequently and are commercially available. either label can be easily incorporated into dna probes and be detected colorimetrically; a number of fluorochromes, as well as alkaline phosphatase and horseradish peroxidase (which produce colored precipitates) are available directly conjugated to anti-digoxigenin antibodies and to avidin. chemiluminescent detection methods and ... | 2001 | 18265226 |
| immunoblotting and immunodetection. | immunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. this unit provides protocols for all steps starting with solubilization of the protein samples, usually with sds and reducing agents. following solubilization, the material is separated by sds-page and the antigens are electrophoretically transferred to a membrane, a process that can be monitored by reversible staining or ponceau s staining. the transferred proteins are boun ... | 2004 | 18265338 |
| in situ hybridization and detection using nonisotopic probes. | nonisotopic in situ hybridization can be used to determine the cellular location and relative levels of expression for specific transcripts within cells and tissues. rna in specimen preparations is hybridized with a biotin- or digoxigenin-labeled probe, which is generally detected by fluorescence or enzymatic methods. fluorescence in situ hybridization (fish), probably the most widely used method, is described here, along with amplification of weak fish signals. nonisotopic probes can also be de ... | 2007 | 18265392 |
| changes in macromolecular transport appear early in caco-2 cells infected with a human rotavirus. | rotavirus is a major cause of viral gastroenteritis, but its interaction with intestinal mucosa is poorly understood. the aim of this study was to examine the effect of wa rotavirus (vp7 serotype 1) on barrier function in confluent caco-2 cell monolayers. wa is the most common serotype causing severe diarrhoea in humans. material and methods. we examined light and electron microscopic morphology, macromolecular transport, paracellular permeability, electrical parameters, disaccharidases and cyto ... | 2008 | 18266175 |
| dna-directed synthesis of aniline and 4-aminobiphenyl oligomers: programmed transfer of sequence information to a conjoined polymer nanowire. | a new class of materials was prepared from aniline-containing oligomers that are covalently linked to the nucleobases of duplex dna. oligomers composed of repeating aniline (pani) or 4-aminobiphenyl (pab) units having the properties of conducting polymers conjoined to the dna were prepared by the reaction of horseradish peroxidase and h2o2 with dna having the appropriate monomers aligned within the major groove. these oligomers exhibit the spectral and chemical properties typical of para-linked ... | 2008 | 18266359 |
| central neural activation of hyperdynamic circulation in portal hypertensive rats depends on vagal afferent nerves. | hyperdynamic circulation in portal hypertensive rats depends on central neural c-fos gene expression, but how the portal hypertensive signal activates central c-fos expression remains obscure. | 2008 | 18270244 |
| a sensitive chemiluminescence method for the determination of h2o2 in exhaled breath condensate. | in this paper, a novel flow injection chemiluminescence (fi-cl) method is proposed for the determination of picomolar l(-1) levels of hydrogen peroxide (h(2)o(2)) in exhaled breath condensate (ebc). this method is based on the oxidation of a low concentration of luminol (10(-7) m) by h(2)o(2) at a low concentration level (< 10(-8) m) in an alkaline medium catalyzed by a complex, k(5)[cu(hio(6))(2)] (dpc), which is not interfered by other metal ions or horseradish peroxidase (hrp). under the opti ... | 2008 | 18270409 |
| a comparative study of free and immobilized soybean and horseradish peroxidases for 4-chlorophenol removal: protective effects of immobilization. | horseradish peroxidase (hrp) and soybean peroxidase (sbp) were covalently immobilized onto aldehyde glass through their amine groups. the activity yield and the protein content for the immobilized sbp were higher than for the immobilized hrp. when free and immobilized peroxidases were tested for their ability to remove 4-chlorophenol from aqueous solutions, the removal percentages were higher with immobilized hrp than with free hrp, whereas immobilized sbp needs more enzyme to reach the same con ... | 2008 | 18270748 |
| utilizing a cdte quantum dots-enzyme hybrid system for the determination of both phenolic compounds and hydrogen peroxide. | in this paper, we attempt to construct a simple and sensitive detection method for both phenolic compounds and hydrogen peroxide, with the successful combination of the unique property of quantum dots and the specificity of enzymatic reactions. in the presence of h2o2 and horseradish peroxidase, phenolic compounds can quench quantum dots' photoluminescence efficiently, and the extent of quenching is severalfold to more than 100-fold increase. quinone intermediates produced from the enzymatic cat ... | 2008 | 18271509 |
| one of the possible mechanisms for the inhibition effect of tb(iii) on peroxidase activity in horseradish (armoracia rusticana) treated with tb(iii). | one of the possible mechanisms for the inhibition effect of tb(iii) on peroxidase activity in horseradish (armoracia rusticana) treated with tb(iii) was investigated using some biophysical and biochemical methods. firstly, it was found that a large amount of tb(iii) can be distributed on the cell wall, that some tb(iii) can enter into the horseradish cell, indicating that peroxidase was mainly distributed on cell wall, and thus that tb(iii) would interact with horseradish peroxidase (hrp) in the ... | 2008 | 18274791 |
| activity increase of horseradish peroxidase in the presence of magnetic particles. | 2008 | 18275197 | |
| identification of l-plastin autoantibody in plasma of patients with non-hodgkin's lymphoma using a proteomics-based analysis. | the diagnosis of malignant lymphoma (ml) such as non-hodgkin's lymphoma (nhl) and hodgkin's lymphoma (hl) was mainly performed by morphological examination and gene analysis. there are only a few serum/plasma biomarkers such as lactate dehydrogenase and soluble interleukin-2 receptor alpha to diagnose ml. the classifications are various, and therefore the cell surface markers using flow cytometry or lymph node biopsy have been examined. it is difficult, however, to distinguish the two diseases, ... | 2008 | 18275676 |
| facile snp detection using bifunctional, cross-linking oligonucleotide probes. | a facile, sensitive method for detecting specific sequences of oligonucleotides was developed. detection of dna sequences with single nucleotide discrimination is achieved by combining the selectivity of hybridization with an efficient cross-linking reaction. readily synthesized bifunctional oligonucleotide probes containing a modified pyrimidine that is capable of forming interstrand cross-links under mild oxidative conditions internally, and biotin at their 5'-termini were used to discriminate ... | 2008 | 18281702 |
| altered blood-brain barrier integrity in adult aquaporin-4 knockout mice. | to investigate the role of astroglial water channel aquaporin-4 (aqp4) in maintaining blood-brain barrier integrity, structure and permeability of the brain microvessels were investigated in adult aqp4 knockout mice. altered ultrastructure of brain microvessels, including open tight junctions and swollen perivascular astrocytic endfeet, were frequently observed in the aqp4 null mice. likewise, aqp4 deficiency significantly downregulated expression of glial fibrillary acidic protein in perivascul ... | 2008 | 18281883 |
| methodologies for staining and visualisation of beta-galactosidase in mouse embryos and tissues. | this chapter provides information on beta-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. using these protocols, localization of beta-galactosidase can be visualized throughout development and in adult tissues. beta-galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies. | 2007 | 18287634 |
| theoretical modeling of enzyme reactions: the thermodynamics of formation of compound 0 in horseradish peroxidase. | in this paper, by using the perturbed matrix method (pmm) in combination with basic statistical mechanical relations both based on nanosecond time-scale molecular dynamics (md) simulations, we quantitatively address the thermodynamics of compound 0 (cpd 0) formation in horseradish peroxidase (hrp) enzyme. our results, in the same trend of low-temperature experimental data, obtained in cryoenzymology studies indicate that such a reaction can be described essentially as a stepwise spontaneous proc ... | 2008 | 18288829 |
| the co-catalytic effect of chlorpromazine on peroxidase-mediated oxidation of melatonin: enhanced production of n1-acetyl-n2-formyl-5-methoxykynuramine. | accumulating evidence points to relationships between increased production of reactive oxygen or decreased antioxidant protection in schizophrenic patients. chlorpromazine (cpz), which remains a benchmark treatment for people with schizophrenia, has been described as a pro-oxidant compound. because the antioxidant compound melatonin exerts protective effects against cpz-induced liver disease in rats, in this investigation, our main objective was to study the effect of cpz as a co-catalyst of per ... | 2008 | 18289161 |
| pathological and biochemical alterations of astrocytes in ovariectomized rats injected with d-galactose: a potential contribution to alzheimer's disease processes. | astrocytes are implicated in the pathological changes of alzheimer's disease. our previous studies have demonstrated that estrogen deprivation and oxidative stress act synergistically to accelerate the progress of alzheimer's disease. long-term d-galactose injection combined with ovariectomy may serve as a rodent model for alzheimer's disease. to address the potential contribution of astroglia to the alzheimer's disease pathogenesis, we investigated pathological and biochemical alterations of as ... | 2008 | 18289535 |
| [removal of pentachlorophenol catalyzed by immobilized horseradish peroxidase]. | the removal of pcp using hrp immobilized by fe3o4 sorption-gelatin embedding-cross linkage method as catalyzer was studied. reaction conditions including the reacting time, different buffer systems and ph value, pcp initial concentration, hrp dosage were discussed in detail compared with free hrp. the results indicate that the equilibrium time of pcp removal reaction catalyzed by immobilized hrp is about 30 min, which is as fast as free hrp. the optimal ph for pcp removal by immobilized hrp is b ... | 2007 | 18290430 |
| preparation and evaluation of microparticles from thiolated polymers via air jet milling. | microparticles were formulated by incorporation of the model protein horseradish peroxidase in (thiolated) chitosan and (thiolated) poly(acrylic acid) via co-precipitation. dried protein/polymer complexes were ground with an air jet mill and resulting particles were evaluated regarding size distribution, shape, zeta potential, drug load, protein activity, release pattern, swelling behaviour and cytotoxicity. the mean particle size distribution was 0.5-12 microm. non-porous microparticles with a ... | 2008 | 18294828 |
| amperometric immunosensor for diagnosis of blv infection. | a new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. the detection of antibody-antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (hrp). ferrocenecarboxylic acid (fca) and n,n,n',n'-tetramethylbenzidine (tmb) were selected as suitable mediators for this immunosensor. optimal conditions for amperometric detection were found. sensitivity of created system was compared with the re ... | 2008 | 18294837 |
| in vitro metabolism of a model cyclopropylamine to reactive intermediate: insights into trovafloxacin-induced hepatotoxicity. | trovafloxacin (trovan) is a fluoroquinolone antibiotic drug with a long half-life and broad-spectrum activity. since its entry into the market in 1998, trovafloxacin has been associated with numerous cases of hepatotoxicity, which has limited its clinical usefulness. trovafloxacin possesses two substructural elements that have the potential to generate reactive intermediates: a cyclopropylamine moiety and a difluoroanilino system. the results presented here describe the in vitro metabolic activa ... | 2008 | 18298092 |
| photocrosslinked anhydride systems for long-term protein release. | injectable delivery systems are attractive as vehicles for localized delivery of therapeutics especially in the context of regenerative medicine. in this study, the potential of photocrosslinked polyanhydride (pa) networks as an encapsulation matrix for long-term delivery of macromolecules was studied. the in vitro release of two model proteins (horseradish peroxidase (hrp) and bovine serum albumin labeled with fluorescein isothiocyanate (fitc-bsa)) was evaluated from crosslinked networks compos ... | 2008 | 18299148 |
| oxidase-peroxidase reaction: kinetics of peroxidase-catalysed oxidation of 2-aminophenol. | possible reaction pathways that may lead to horseradish peroxidase inactivation during the aerobic oxidation of 2-aminophenol were investigated using extended kinetic curves. a kinetic model involving the formation of a low-reactive species, compound iii, was proposed and several rate constants were calculated using an optimisation computing program. sensitivity analysis allowed to conclude that both oxidase and peroxidase cycles occur in 2-aminophenol oxidation. | 2008 | 18299896 |
| detection of uch-l1 expression by pre-embedding immunoelectron microscopy with colloidal gold labeling in diseased glomeruli. | the purpose of this report was to study the use of pre-embedding immunoelectron microscopy technique with gold and horseradish peroxidase (hrp) labeling in detecting the expression of ubiquitin c-terminal hydrolase 1 (uch-l1) of podocytes in glomerulonephritis. the specimens of human iga nephropathy and lupus nephritis were fixed with paraformaldehyde and lysine-hcl buffer, labeled by colloidal gold or hrp, embedded with epoxy resin, and examined under the transmission electron microscope. the h ... | 2008 | 18300032 |
| a novel and highly sensitive resonance scattering spectral assay for horseradish peroxidase using cationic surfactant. | a novel and ultrasensitive resonance scattering (rs) spectral assay was proposed for detection of horseradish peroxidase (hrp) activity. it was based on that the hrp strongly catalyze h2o2 oxidation of excess i- to form i3-, the resulting i3- combined with four cationic surfactant (cs), including tetradecyl pyridinium bromide (tpb), cetyltrimethyl ammonium bromide (ctma), cetylpyridinium chloride (cpcm) and tetradecyl dimethylbenzyl ammonium chloride (tdmba) to produce association particles (tpb ... | 2008 | 18302009 |