Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| correlations between tissue-level stresses and strains and cellular damage within the guinea pig spinal cord white matter. | strain magnitude, strain rate, axon location, axon size, and the local tissue stress state have been proposed as the mechanisms governing primary cellular damage within the spinal cord parenchyma during slow compression injury. however, the mechanism of axon injury has yet to be fully elucidated. the objective of this study was to correlate cellular damage within the guinea pig spinal cord white matter, quantified by a horseradish peroxidase (hrp) exclusion test, with tissue-level stresses and s ... | 2007 | 17675041 |
| novel enzymatic properties of dna-pt complexes. | dna-pt complexes have shown novel enzymatic activity as a peroxidase similar to that of horseradish peroxidase in the colorimetric reaction with its substrate. the enzymatic activity of these complexes increased with increasing reaction time and ph in reaction solutions of dna and k2[ptcl4]. this enhanced enzymatic activity was attributed to the increase in pt conjugated to dna in the complex. the enzymatic activity per unit mole of the dna-pt complex was significantly higher for complexes prepa ... | 2007 | 17676798 |
| disruption of ha_btr alters binding of bacillus thuringiensis delta-endotoxin cry1ac to midgut bbmvs of helicoverpa armigera. | disruption of the ha_btr (a cadherin gene) is genetically linked to resistance to cry1ac delta-endotoxin of bacillus thuringiensis in the gybt strain of helicoverpa armigera. brush border membrane vesicles (bbmvs) prepared from midguts of both the cry1ac-resistant gybt strain (homozygous for a deletion knockout of ha_btr) and the susceptible gy strain (homozygous for the wild type of ha_btr) possessed saturable and specific binding ability to (125)i-cry1ac. the binding constant (k(d)) of the gy ... | 2008 | 17681529 |
| generation and identification of reactive metabolites by electrochemistry and immobilized enzymes coupled on-line to liquid chromatography/mass spectrometry. | the detection of reactive metabolites using conventional in vivo and in vitro techniques is hampered because the intermediately formed reactive species are prone to covalent binding to cellular macromolecules. therefore, the application of improved methods is required. the on-line coupling of an electrochemical reactor and horseradish peroxidase immobilized on magnetic microparticles with liquid chromatography/mass spectrometry (ec/lc/ms or hrp/lc/ms) allows the direct detection of reactive meta ... | 2007 | 17685550 |
| intracellular labeling of single cortical astrocytes in vivo. | glial cells have traditionally been considered to play supportive roles in the central nervous system. as recent experimental evidence suggests glial cells' participation in neural information processing, there has been a need to monitor the physiology of glial cells in vivo in the matured brain. concurrently, identification and classification of the recorded glial cells is essential as there are at least several different kinds of glial cells. past studies have achieved in vivo intracellular el ... | 2007 | 17686526 |
| generation and application of a fluorescein-specific single chain antibody. | a recombinant single chain antibody fragment (designated scde1) of the murine monoclonal anti-fluorescein antibody b13-de1 was generated using the original hybridoma cells as source for the variable antibody heavy and light chain (vh and vl) genes. after cloning the variable genes into a phage vector a functional antibody fragment was selected by phage display panning. recombinant antibody could be expressed as phage antibody and as soluble single chain antibody in escherichia coli. high yield o ... | 2007 | 17686564 |
| a sensitive immunoassay for determination of hepatitis b surface antigen and antibody in human serum using capillary electrophoresis with chemiluminescence detection. | a sensitive and homogeneous immunoassay (ia) based on capillary electrophoresis (ce) with enhanced chemiluminescence (cl) detection has been developed for the determination of hepatitis b surface antigen (hbsag) and antibody (hbsab) in human serum. the conditions for the cl reaction and electrophoresis were investigated in detail using horseradish peroxidase (hrp) labeled hbsag (hbsag*) as a marker because of its catalytic effects on the luminol-hydrogen peroxide reaction. the cl reaction was en ... | 2007 | 17689153 |
| synthesis of enzymatically-gellable carboxymethylcellulose for biomedical applications. | we synthesized a carboxymethylcellulose with phenol moieties by covalently incorporating tyramine into carboxymethylcellulose using aqueous-phase carbodiimide activation chemistry. the resulting hydrogel was obtained from an aqueous solution of the conjugate via the horseradish peroxidase-catalyzed oxidation reaction of phenols by consuming h(2)o(2), where the gelation speed depended on the concentrations of enzyme and h(2)o(2). the viability of the mammalian cells enclosed within the hydrogel p ... | 2007 | 17697980 |
| application of the paramagnetic dipole field for solution nmr active site structure determination in low-spin, cyanide-inhibited ferric hemoproteins. | the principles for the application of the paramagnetic dipolar field of low-spin, cyanide-inhibited ferrihemoproteins for determining active site structure are briefly described. the ubiquitous dipolar shifts for assigned residues, together with crystal coordinates of some appropriate structural homolog, allow determination of the orientation and anisotropies of the paramagnetic dipolar tensor. the orientation of chi uniquely defines the orientation of the fe-cn unit, which is tilted variably an ... | 2007 | 17701546 |
| impaired deoxyribonuclease activity in monoglandular and polyglandular autoimmunity. | the enzyme desoxyribonuclease (dnase) degrades dna during early apoptosis. impaired dnase activity might increase susceptibility to autoimmune diseases. this study examined for the first time dnase activity in endocrine autoimmunity. | 2007 | 17701885 |
| ethanolic crude extract and flavonoids isolated from alternanthera maritima: neutrophil chemiluminescence inhibition and free radical scavenging activity. | extracts from alternanthera maritima are used in brazilian folk medicine for the treatment of infectious and inflammatory diseases. bioassay-guided fractionation of a. maritima aerial parts yielded an ethanolic crude extract, its butanolic fraction and seven isolated flavonoids (two aglycones, two o-glycosides and three c-glycosides) with antioxidative activity. the ability of these samples to scavenge enzymatically generated free radicals (luminol-horseradish peroxidase-h2o2 reaction) and inhib ... | 2007 | 17708437 |
| enzyme-based colorimetric detection of nucleic acids using peptide nucleic acid-immobilized microwell plates. | the development of label-free or nonlabeling assays for nucleic acids is important in basic biological research and biomedical diagnosis. in this study, we have developed an enzyme-based colorimetric assay for nucleic acids, which combines the robustness of nonlabeling of dna and rna samples and the adequate sensitivity of enzymatic reactions. the core of this assay is the use of neutral peptide nucleic acid (pna) as capture probe and the electrostatic adsorption of horseradish peroxidase (hrp) ... | 2007 | 17708676 |
| channel and substrate zone two-dimensional resolution for chemiluminescent multiplex immunoassay. | a two-dimensional resolution system of channels and substrate zones was proposed for multiplex immunoassay performed with a designed multichannel chemiluminescent (cl) detection device coupled with a single photomultiplier. using carcinoma antigen 125 (ca 125), carcinoma antigen 153 (ca 153), carcinoma antigen 199 (ca 199), and carcinoembryonic antigen (cea) as two couples of model analytes, two couples of capture antibodies were immobilized in two channels, respectively. with a sandwich format, ... | 2007 | 17713968 |
| anatomic and physiological characteristics of the ferret lateral rectus muscle and abducens nucleus. | the ferret has become a popular model for physiological and neurodevelopmental research in the visual system. we believed it important, therefore, to study extraocular whole muscle as well as single motor unit physiology in the ferret. using extracellular stimulation, 62 individual motor units in the ferret abducens nucleus were evaluated for their contractile characteristics. of these motor units, 56 innervated the lateral rectus (lr) muscle alone, while 6 were split between the lr and retracto ... | 2007 | 17717110 |
| [efferent innervation of the cervical region of the trachea during the early postnatal ontogenesis]. | method based on retrograde axonal transport of horseradish peroxidase was used to study the efferent innervation of the cervical trachea in neonatal, 10-, 20-, 30-day-old and two-month-old kittens. in all the animals, labeled neurons were localized in the cranial cervical, middle cervical and cervicothoracic (stellate) sympathetic ganglia both on the right and on the left sides. neurons were also localized at the level of medulla oblongata, in the dorsal and ambiguus nuclei of the vagus nerve. l ... | 2007 | 17722570 |
| a randomised, double-blind, placebo-controlled trial of a herbal medicinal product containing tropaeoli majoris herba (nasturtium) and armoraciae rusticanae radix (horseradish) for the prophylactic treatment of patients with chronically recurrent lower urinary tract infections. | the aim of this study was to verify the efficacy and safety of a herbal medicinal product containing tropaeoli majoris herba and armoraciae rusticanae radix in the prophylactic treatment of chronically recurrent urinary tract infections (utis), and to test whether the medicinal product decreases the incidence of relapses over the study period. | 2007 | 17723159 |
| self-assembling gold nanoparticles on thiol-functionalized poly(styrene-co-acrylic acid) nanospheres for fabrication of a mediatorless biosensor. | a novel strategy to construct a sensitive mediatorless sensor of h2o2 was described. at first, a cleaned gold electrode was immersed in thiol-functionalized poly(styrene-co-acrylic acid) (st-co-aa) nanosphere latex prepared by emulsifier-free emulsion polymerization st with aa and function with dithioglycol to assemble the nanospheres, then gold nanoparticles were chemisorbed onto the thiol groups and formed monolayers on the surface of poly(st-co-aa) nanospheres. finally, horseradish peroxidase ... | 2006 | 17723393 |
| bulk-modified modified screen-printing carbon electrodes with both lactate oxidase (lod) and horseradish peroxide (hrp) for the determination of l-lactate in flow injection analysis mode. | a screen-printed carbon electrode modified with both hrp and lod (spce-hrp/lod) has been developed for the determination of l-lactate concentration in real samples. the resulting spce-hrp/lod was prepared in a one-step procedure, and was then optimised as an amperometric biosensor operating at [0, -100]mv versus ag/agcl for l-lactate determination in flow injection mode. a significant improvement in the reproducibility (coefficient variation of about 10%) of the preparation of the biosensors was ... | 2006 | 17723394 |
| application of sio2-poly(dimethylsiloxane) hybrid material in the fabrication of amperometric biosensor. | silica xerogel was widely used in the development of biosensors by coupling the desired biological components to various transducers. unfortunately, the application of xerogels is limited due to their poor mechanical properties and poor structural maintenance of entrapped biomaterials. in this study, the availability of poly(dimethylsiloxane) (pdms)-modified silica sol-gel (teos/pdms ormosil) glass in the immobilization of enzymes and its application in the fabrication of amperometric biosensor ... | 2006 | 17723485 |
| a comprehensive immunoassay for the detection of microcystins in waters based on polyclonal antibodies. | microcystins (mcs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue-green algae), and microcystin-leucine-arginine (mc-lr) is among the most frequent and most toxic microcystin congeners. in this study, a free amino group was introduced to mc-lr at its seventh amino acid residue with 2-mercaptoethylamine, and the product aminoethyl-mc-lr was coupled to bovine serum albumin (bsa) and horseradish peroxidise (hrp) by glutaraldehyde to be complete anti ... | 2006 | 17723494 |
| enzymatic methods in food analysis: determination of ascorbic acid. | the feasibility and expediency of enzymatic methods application in food analysis is demonstrated by the example of ascorbic acid (asa) determination in foods. enzymatic determination of ascorbic acid is based on its action as a second substrate of horseradish (hrp) and peanut (pnp) peroxidases in the reactions of o-dianisidine (od) and 3,3',5,5'-tetramethylbenzidine (tmb) oxidation with hydrogen peroxide. the rates of the reactions are monitored spectrophotometrically by measuring the duration o ... | 2006 | 17723515 |
| enhancement of a conducting polymer-based biosensor using carbon nanotube-doped polyaniline. | a biosensor with improved performance was developed through the immobilization of horseradish peroxidase (hrp) onto electropolymerized polyaniline (pani) films doped with carbon nanotubes (cnts). the effects of electropolymerization cycle and cnt concentration on the response of the biosensor toward h2o2 were investigated. it was found that the application of cnts in the biosensor system could increase the amount and stability of the immobilized enzyme, and greatly enhanced the biosensor respons ... | 2006 | 17723569 |
| development of a chemiluminescent immunosensor for chloramphenicol. | a direct competitive chemiluminescent immunosensor system that exploits the competition between chloramphenicol (cap) as an analyte and cap-horseradish peroxidase conjugate as a tracer for binding to an anti-cap antibody on a solid support was devised by installing a flow-through cell which was connected to an injector and a peristaltic pump inside a dark box, followed by positioning a photomultiplier tube as light detector in front of it. the anti-cap antibody was immobilized onto positively ch ... | 2006 | 17723690 |
| flow-injection immuno-bioassay for interleukin-6 in humans based on gold nanoparticles modified screen-printed graphite electrodes. | a flow-injection electrochemical immunoassay system based on a disposable immunosensor for the determination of interleukin-6 (il-6) was proposed. the immunosensor was prepared by entrapping horseradish peroxidase (hrp)-labeled il-6 antibody into gold nanoparticles-modified composite membrane at a screen-printed graphite electrode. with a non-competitive immunoassay format, the immunosensor was inserted in the flow system with an injection of sample, and the injected sample containing il-6 antig ... | 2006 | 17723764 |
| commissural mirror-symmetric excitation and reciprocal inhibition between the two superior colliculi and their roles in vertical and horizontal eye movements. | the functional roles of commissural excitation and inhibition between the two superior colliculi (scs) are not yet well understood. we previously showed the existence of strong excitatory commissural connections between the rostral scs, although commissural connections had been considered to be mainly inhibitory. in this study, by recording intracellular potentials, we examined the topographical distribution of commissural monosynaptic excitation and inhibition from the contralateral medial and ... | 2007 | 17728384 |
| the action of horseradish-peroxidase on angiotonin, pepsitensin and epinephrine. | (1) the pressor action of angiotonin, pepsitensin and epinephrine is rapidly destroyed by the horseradish-peroxidase-hydrogen peroxide system. (2) this reaction is considerably enhanced by the addition of small amounts of potassium iodide. | 1945 | 17730622 |
| de gustibus non est disputandem: a spiral center for taste in the brain of the teleost fish, heterotis niloticus. | the teleost fish, heterotis niloticus, has elaborate paired, spiraled pharyngeal structures that aid in concentrating and swallowing food. these epibranchial organs are lined by an epithelium rich in taste buds. both the taste buds and the muscles of the epibranchial organs are innervated by components of the vagal nerve. horseradish peroxidase neuronal tracing experiments show that these nerve fibers are connected centrally to an enormous epibranchial portion of the vagal lobes-a special viscer ... | 1986 | 17743572 |
| bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode. | a bienzymatic glucose biosensor was proposed for selective and sensitive detection of glucose. this mediatorless biosensor was made by simultaneous immobilization of glucose oxidase (god) and horseradish peroxidase (hrp) in an electropolymerized pyrrole (ppy) film on a single-wall carbon nanotubes (swnt) coated electrode. the amperometric detection of glucose was assayed by potentiostating the bienzymatic electrode at -0.1 versus ag/agcl to reduce the enzymatically produced h(2)o(2) with minimal ... | 2007 | 17764922 |
| conducting polymer based fluorescence quenching as a new approach to increase the selectivity of immunosensors. | polypyrrole (ppy) has been shown to be a superior matrix for fluorescence detection based immunosensors: (i) the fluorescence of polypyrrole and polypyrrole modified by entrapped proteins was almost not detectable when this polymer was excited by near uv 325 nm light; (ii) polypyrrole quenched the fluorescence of such fluorescence agents as fluoresceine 5(6)-isothiocyanate, rhodamine b and enzyme-horseradish peroxidase (hrp) by almost 100% if they were deposited in the solution as a drop at the ... | 2007 | 17764923 |
| peroxidase activity of de novo heme proteins immobilized on electrodes. | de novo proteins from designed combinatorial libraries were bound to heme terminated gold electrodes. the novel heme proteins were shown to possess peroxidase activity, and this activity was compared to that of horseradish peroxidase and bovine serum albumin when immobilized in a similar fashion. the various designed proteins from the libraries displayed distinctly different levels of peroxidase activity, thereby demonstrating that the sequence and structure of a designed protein can exert a sub ... | 2007 | 17765314 |
| non-competitive immunoassay for luteinizing hormone in human serum using capillary electrophoresis with chemiluminescence detection. | a non-competitive immunoassay based on capillary electrophoresis (ce) with chemiluminescence (cl) detection has been developed for the determination of luteinizing hormone (lh) in human serum. the work involved the development of separation and cl conditions, allowing for routine analysis of serum samples. in this study, horseradish peroxidase (hrp)-labelled monoclonal anti-lh can catalyse the luminol-hydrogen peroxide reaction. the determined lh can react with excessive amount of hrp-labelled a ... | 2007 | 17768703 |
| monospecific and bispecific antibodies against e. coli o157 for diagnostics. | escherichia coli o157:h7 is a serious human pathogen that causes hemorrhagic colitis, and occasionally hemolytic uremic syndrome. identification of the o157 antigen is an essential part of the detection and management of e. coli o157:h7. a quadroma p126 secreting a bispecific hybrid mab (bsmab), which recognizes both e. coli o157 and horseradish peroxidase in one molecule was produced by somatic hybridization of hybridomas specific for e. coli o157 and hrpo molecule. a bridge elisa was used to s ... | 2007 | 17804009 |
| plasma immersion ion implantation treatment of polyethylene for enhanced binding of active horseradish peroxidase. | robust attachment of active proteins to synthetic surfaces underpins the development of biosensors and protein arrays. this paper presents the results of experiments in which energetic ions, extracted from an inductively coupled nitrogen plasma, are used to modify the surface of ultrahigh molecular weight polyethylene (uhmwpe). the ability of the surface to bind active horseradish peroxidase (hrp) is significantly enhanced by the plasma treatment. the amide signal in infrared spectroscopy indica ... | 2008 | 17806119 |
| peroxidase-catalyzed removal of phenols from coal-conversion waste waters. | a novel, enzymatic approach has been developed for the removal of phenols from coal-conversion aqueous effluents. treatment with horseradish peroxidase and hydrogen peroxide precipitates 97 to 99 percent of the phenol in a wide range of ph and phenol concentrations; both model mixtures and real industrial waste-water samples have been treated successfully. other pollutants, such as polychlorinated biphenyls, can be enzymatically coprecipitated with the phenols. | 1983 | 17815191 |
| an enzyme-linked immunosorbent assay (elisa) to measure growth hormone level in serum and milk of buffaloes (bubalus bubalis). | an indirect sandwich elisa to measure growth hormone level in serum and milk of buffaloes was developed. the assay was based on purified anti rbst igg raised in rabbits and chicken and rabbit anti chicken igg horseradish peroxidase. the assay was validated in terms of sensitivity, specificity, precision and recovery. parallelism was demonstrated between the standard curve and serially diluted serum, milk and pituitary derived growth hormone. sensitivity of the assay was 0.1 ng/ml. recovery of ex ... | 2007 | 17821853 |
| lacz transgenic mice and immunoelectron microscopy: an ultrastructural method for dual localization of beta-galactosidase and horseradish peroxidase. | transgenic animals bearing the reporter gene, lacz, encoding the histochemical enzyme, beta-galactosidase, are increasingly becoming available. similarly, antibody conjugates consisting of specific iggs coupled to horseradish peroxidase (hrp) are widely used for western blotting, elisa, and immunohistochemistry. here we provide a detailed fixation and histochemical protocol for the simultaneous electron microscopic visualization and discrimination of beta-galactosidase and peroxidase reaction pr ... | 2007 | 17827164 |
| development of an elisa method for detecting immune complexes between tissue-nonspecific alkaline phosphatase and immunoglobulin g. | a convenient method for measuring immune complexes between tissue-nonspecific alkaline phosphatase (tnsalp) and immunoglobulin g (igg) (i.e., tnsalp-igg) would be highly useful for routine analyses. here, we identified a surface-active agent that would dissolve membrane but not dissociate tnsalp-igg complexes. next, we developed an enzyme-linked immunosorbent assay (elisa) method for detecting tnsalp-igg complexes with two monoclonal antibodies (moabs): 3-29-3r was coated on assay plates and cap ... | 2007 | 17847105 |
| dihydroxy bile acids increase mucosal permeability and bacterial uptake in human colon biopsies. | bile acids in mm concentrations are known to increase chloride secretion and alter mucosal permeability in animal colon. increased mucosal permeability is believed to play an important role in the development of intestinal inflammation. the aim of this study was to investigate the influence of microm concentrations of dihydroxy bile acids on permeability and bacterial uptake in the normal human colon. | 2007 | 17852874 |
| the utility of smad4 as a diagnostic immunohistochemical marker for pancreatic adenocarcinoma, and its expression in other solid tumors. | pancreatic adenocarcinoma is a genetic disease showing somatic mutations of multiple genes, including smad4. smad4 is a tumor suppressor gene that is inactivated in a sub-set of pancreatic adenocarcinoma, either by the intragenic mutation of one allele in combination with the loss of the other allele or by homozygous deletion of both alleles. this study examines smad4 expression in fine-needle aspiration cell blocks from patients with pancreatic adenocarcinoma, as well as a variety of human canc ... | 2007 | 17854080 |
| highly sensitive second-antibody enzyme immunoassay for determination of estradiol-17beta concentration in blood plasma of the mithun (bos frontalis). | the objective of the present study was to develop and validate a simple, sensitive, quick and economic enzyme immunoassay (eia) for estradiol-17beta (e2) in mithun (bos frontalis) plasma on microtiter plates using a second-antibody coating technique and hormone-horseradish peroxidase as a label. for the assay, the wells of microtiter plates were coated with affinity-purified goat anti-rabbit igg that binds the hormone-specific antibody. one milliliter of mithun plasma was extracted using benzene ... | 2007 | 17867839 |
| electrochemical immunosensor for carcinoembryonic antigen based on antigen immobilization in gold nanoparticles modified chitosan membrane. | a disposable electrochemical immunosensor for carcinoembryonic antigen (cea) was proposed based on the antigen immobilized in a colloidal gold nanoparticles modified chitosan membrane on the surface of an indium-tin oxide (ito) electrode. the different membranes were characterized by scanning electron microscope and electrochemical methods. based on a competitive immunoassay format, the immobilized antigen of the immunosensor was incubated with a horseradish peroxidase (hrp) labeled antibody and ... | 2007 | 17878578 |
| development of a quantitative bio/chemiluminescence spectrometer determining quantum yields: re-examination of the aqueous luminol chemiluminescence standard. | we have developed a bio/chemiluminescence spectrometer with a cooled charge-coupled-device (ccd) detector to obtain a quantitative luminescence spectrum as the absolute number of all emitted photons at each wavelength. the integrated area of the spectrum divided by the number of reacted substrate molecules gives the quantum yield. calibration of the absolute sensitivity of the ccd-spectrometer system was performed by using lasers and a tungsten lamp with calibrated powers as primary light standa ... | 2013 | 17880516 |
| light-driven horseradish peroxidase cycle by using photo-activated methylene blue as the reducing agent. | in this work, the regeneration of native horseradish peroxidase (hrp), following the consecutive reduction of oxo-ferryl pi-cation (compound i) and oxo-ferryl (compound ii) forms, was observed by uv-visible spectrometry and electron paramagnetic resonance (epr) in the presence of methylene (mb+), in the dark and under irradiation. in the dark, mb+ did not affect the rate of hrp compound i and ii reduction, compatible with hydrogen peroxide as the solely reducing species. under irradiation, the d ... | 2013 | 17880521 |
| x-ray crystal structural analysis of the binding site in the ferric and oxyferrous forms of the recombinant heme dehaloperoxidase cloned from amphitrite ornata. | the dehaloperoxidase (dhp) from the terebellid polychaete amphitrite ornata is an enzyme that converts para-halogenated phenols to the corresponding quinones in the presence of hydrogen peroxide. its enzymatic activity is similar to that of heme peroxidases such as horseradish peroxidase, yet it has the structural characteristics of the globin family of proteins, the main functions of which are oxygen transport and storage. in order to investigate the dual function of this hemoglobin peroxidase, ... | 2007 | 17881827 |
| enhancing the sensitivity and stability of hrp/pani/pt electrode by implanted bovine serum albumin. | polyaniline (pani) is considered as one of the most fascinating conductive polymers in fabricating enzyme-based biosensors. nevertheless, to improve both sensitivity and stability of the pani-modified biosensor has been and continues to be a technical challenge. in this study, we have electrochemically synthesized the pani film on a platinum (pt) electrode and then used this electrode to construct a horseradish peroxidase (hrp)-based biosensor for the detection of hydrogen peroxide (h(2)o(2)). t ... | 2008 | 17890076 |
| in vivo prophylactic activity of qr-435 against h3n2 influenza virus infection. | prophylaxis against influenza infection can take several forms, none of which is totally effective at preventing the spread of the disease. qr-435, an all-natural compound of green-tea extract and other agents, has been developed to protect against a range of viral infections, including the influenza subtype h3n2. | 2007 | 17890936 |
| oxidation chemistry of norepinephrine: partitioning of the o-quinone between competing cyclization and chain breakdown pathways and their roles in melanin formation. | aberrant oxidation of norepinephrine (1) via the transient o-quinone has been implicated as a critical pathogenetic mechanism underlying the degeneration of noradrenergic cell bodies in the locus coeruleus in parkinson's disease, the degeneration of noradrenergic nerve terminals in alzheimer's disease and following transient cerebral ischemia, and the onset and progression of idiopathic vitiligo. an oxidative pathway of 1 is also believed to account for the slow deposition of neuromelanin in pig ... | 2007 | 17892264 |
| removal of estrogenic activity of natural and synthetic hormones from a municipal wastewater: efficiency of horseradish peroxidase and laccase from trametes versicolor. | some researches studied the removal of steroid estrogens by enzymatic treatment, however none verified the residual estrogenicity after the enzymatic treatment at environmental conditions. in this study, the residual estrogenic activities of the key natural and synthetic steroid estrogens were investigated following enzymatic treatment with horseradish peroxidase (hrp) and laccase from trametes versicolor. synthetic water and municipal wastewater containing environmental concentrations of estron ... | 2008 | 17897698 |
| origin and development of unusual insect muscle tension receptors. | this work describes the origin and development of about 200 tension receptor cells located around the anterior attachment site of the locust ovipositor muscle and their migration to their final position on the muscle fibres. the locust ovipositor muscle is the only insect system in which more than 100 tension receptor cells are associated with a single muscle. neuronal precursors of tension receptors are first detectable by horseradish peroxidase immunohistochemistry in fourth instar larvae. pre ... | 2007 | 17899200 |
| sequential injection chemiluminescence immunoassay for nonionic surfactants by using magnetic microbeads. | a rapid and sensitive immunoassay based on a sequential injection analysis (sia) using magnetic microbeads for the determination of alkylphenol polyethoxylates (apneos) is described. an sia system was constructed from a syringe pump, a switching valve, a flow-through type immunoreaction cell equipped with a photon counting unit and a neodymium magnet. magnetic beads, to which an anti-apneos monoclonal antibody was immobilized, were used as a solid support in an immunoassay. the introduction, tra ... | 2007 | 17903471 |
| the determination of hydrogen peroxide generated from cigarette smoke with an ultrasensitive and highly selective chemiluminescence method. | monitoring hydrogen peroxide (h(2)o(2)) in aqueous cigarette smoke condensate (csc) is helpful for interpreting the relationship between cigarette smoke and oxidative stress, inflammation and disease. it is also significative for elucidating the pathogenic effects of csc. in this paper, a novel flow-injection chemiluminescence (fi-cl) method was well established for determination of h(2)o(2) in the complex sample csc which did not need pretreatment. the sensitive and selective method is based on ... | 2007 | 17904474 |
| spring cabbage peroxidases--potential tool in biocatalysis and bioelectrocatalysis. | two fractions of peroxidase activity, cationic px-cat and anionic px-ani, were isolated and partially purified (143.5- and 5.49-fold, respectively) from homogenate of spring cabbage heads. optimum ph for both fractions is 6.0; however, px-cat is almost equally active at neutral ph (7.0) while px-ani reveals high activity in more acidic phs (with 60% of maximum activity at ph 3.0). optimal temperature for both fractions was 40 degrees c. px-ani possessed much higher thermal stability at 40-50 deg ... | 2008 | 17904598 |
| novel technique for estimating cerebrovascular permeability demonstrates capsazepine protection following ischemia-reperfusion. | there has been some discussion as to whether the pial vasculature behaves in the same way as the blood-brain barrier as a whole. recent studies have shown that capsazepine protects these vessels from the effects of ischemia-reperfusion. we have now used a new method to examine this protection in the whole brain. | 2007 | 17907014 |
| a low perfusion rate microreactor for continuous monitoring of enzyme characteristics: application to glucose oxidase. | this report describes a versatile and robust microreactor for bioactive proteins physically immobilized on a polyether sulfone filter. the potential of the reactor is illustrated with glucose oxidase immobilized on a filter with a cut-off value of 30 kda. a flow-injection system was used to deliver the reactants and the device was linked on-line to an electrochemical detector. the microreactor was used for on-line preparation of apoglucose oxidase in strong acid and its subsequent reactivation w ... | 2007 | 17909761 |
| preferential hydroxylation over epoxidation catalysis by a horseradish peroxidase mutant: a cytochrome p450 mimic. | density functional theory calculations are presented on the catalytic properties of a horseradish peroxidase mutant whereby the axial nitrogen atom is replaced by phosphorus. this mutant has never been studied experimentally and only one theoretical report on this system is known (de visser, s. p. j. phys. chem. b 2006, 110, 20759-20761). thus, a one-atom substitution in horseradish peroxidase changes the properties of the catalytic center of the enzyme to more cytochrome p450-type qualities. in ... | 2007 | 17914801 |
| investigation into the signal transduction pathway via which heat stress impairs intestinal epithelial barrier function. | intact protein absorption is thought to be a causative factor in several intestinal diseases, such as food allergy, celiac disease and inflammatory bowel disease. however, the mechanism remains unclear. the aim of this study was to characterize a novel signal transduction pathway via which heat stress compromises intestinal epithelial barrier function. | 2007 | 17914957 |
| application of zero-length cross-linking to form lysozyme, horseradish peroxidase and lysozyme-peroxidase dimers: activity and stability. | a facile method for the formation of covalent bonds between protein molecules is zero-length cross-linking. this method enables the formation of cross-links without use of any chemical reagents. here, the cross-linking is performed for lysozyme, peroxidase (a glycoprotein) and between lysozyme-peroxidase by the method of simons et al. [b.l. simons, m.c. king, t. cyr, m.a. hefford, h. kaplan, covalent cross-linking of protein without chemical reagents, protein sci. 2002, 11, 1558-1564]. approxima ... | 2007 | 17915308 |
| porphyrinoid chemistry in hemoprotein matrix: detection and reactivities of iron(iv)-oxo species of porphycene incorporated into horseradish peroxidase. | 2007 | 17915871 | |
| optical and electrochemical detection of saccharides with poly(aniline-co-3-aminobenzeneboronic acid) prepared from enzymatic polymerization. | boronic acid-based sensors for saccharides have been developed via biocatalysis. the self-doped copolymer of poly(aniline-co-3-aminobenzeneboronic acid) [poly(aniline-co-ab)], with various mole ratios of two components, was synthesized by oxidative enzymatic polymerization using a natural biocatalyst such as horseradish peroxidase together with an anionic polyelectrolyte template (sulfonated polystyrene) under mild conditions (ph 4.5). poly(aniline-co-ab), having an aniline boronic acid-to-anili ... | 2007 | 17918994 |
| distribution and translocation of 141ce (iii) in horseradish. | rare earth elements (rees) are used in agriculture and a large amount of them contaminate the environment and enter foods. the distribution and translocation of (141)ce (iii) in horseradish was investigated in order to help understand the biochemical behaviour and toxic mechanism of rees in plants. | 2007 | 17921527 |
| flow shear stress affects macromolecular accumulation through modulation of internal elastic lamina fenestrae. | internal elastic lamella and elastic lamellae serve as barriers for macromolecular accumulation in vascular wall. the function of internal elastic lamella in atherogenesis induced by shear stress has not been well understood yet. in the present study, internal elastic lamella remodeling and transmural accumulation of macromolecules in the arterial wall under altered shear stress were investigated. | 2008 | 17923177 |
| vascular endothelial growth factor and nitric oxide production in response to hypoxia in the choroid plexus in neonatal brain. | damage to the choroid plexus in 1-day-old wistar rats subjected to hypoxia was investigated. the mrna and protein expression of hypoxia-inducible factor-1alpha (hif-1alpha), endothelial, neuronal, inducible nitric oxide synthase (enos, nnos, inos), and vascular endothelial growth factor (vegf) along with nitric oxide (no) production and vegf concentration was up-regulated significantly in hypoxic rats. ultrastructurally, the choroid plexus epithelial cells showed massive accumulation of glycogen ... | 2008 | 17924979 |
| biocatalytic oxidation of bisphenol a in a reverse micelle system using horseradish peroxidase. | horseradish peroxidase (hrp) was used to catalyze the oxidation of bisphenol a (bpa) in a reverse micelle system consisting of water, sodium bis(2-ethylhexyl)sulfosuccinate (aot) as the surfactant, and n-octane as the organic solvent phase. in order to achieve maximal bpa transformation, a water-to-surfactant molar ratio greater than 15 was required, above which no further increase in conversion was observed. bpa transformation was catalyzed in the reverse micelle system over a ph range of 6-9 w ... | 2008 | 17928223 |
| influence of position of substituent groups on removal of chlorophenols and cresols by horseradish peroxidase and determination of optimum conditions. | enzymatic treatment of o-, m-, and p-chlorophenols and o-, m-, and p-cresols from artificial wastewater was undertaken through the enzymatic conversion into the corresponding phenoxy radicals with horseradish peroxidase (hrp) and nonenzymatic radical coupling reaction. the concentration of chlorophenols and cresols decreased sharply over the reaction time and water-insoluble oligomer precipitates were generated. the optimum conditions were determined to be the h2o2 concentration of 2.5 mm and po ... | 2007 | 17928697 |
| penetration of 38% hydrogen peroxide into the pulp chamber in bovine and human teeth submitted to office bleach technique. | this study evaluated the pulp chamber penetration of peroxide bleaching agent in human and bovine teeth after office bleach technique. all the teeth were sectioned 3 mm apical of the cement-enamel junction and were divided into 2 groups, a (70 third human molars) and b (70 bovine lateral incisors), that were subdivided into a1 and b1 restored by using composite resin, a2 and b2 by using glass ionomer cement, and a3 and b3 by using resin-modified glass ionomer cement; a4, a5, b4, and b5 were not ... | 2007 | 17931936 |
| a quantitative analysis of n-myc downstream regulated gene 2 (ndrg 2) in human tissues and cell lysates by reverse-phase protein microarray. | n-myc downstream regulated gene 2 (ndrg2) belongs to the ndrg family, which is comprised of 4 members, ndrg1-4. recently, ndrg2 was reported as a new candidate for a tumor suppressor gene. we developed a reverse-phase protein microarray assay to access ndrg2 levels in human tissue specimens and cell lines. | 2008 | 17936257 |
| purification, cloning and characterization of a novel peroxidase isozyme from sweetpotatoes (ipomoea batatas). | an anionic peroxidase from sweetpotato tubers is purified and characterized. the isozyme ibprx15 is purified to homogeneity by affinity chromatography using a concanavalin a column. the isoelectric point was determined to pi 4.9. maldi-ms detected a singly charged molecule with a mass of 42029 da. absorption spectra of ibprx15 compounds i, ii and iii were obtained after treatment with h(2)o(2) at room temperature. comparative data of ibprx15 on substrate specificity to tobacco anionic peroxidase ... | 2007 | 17936696 |
| conductometric immunosensors for the detection of staphylococcal enterotoxin b based bio-electrocalytic reaction on micro-comb electrodes. | staphylococcal enterotoxin b (seb) is one of many toxins produced by the gram-positive bacterium staphylococcal aureus. while seb is known as the causative agent of certain food poisonings it is also considered abiological select agent. thus, rapid and accurate identification of seb during either surveillance or in response to a biothreat is critical to the mitigation of the suspect agent. this report presents a new conductometric immune-biosensor for the detection of seb based on immobilization ... | 2008 | 17943320 |
| structure, function, and stability of enzymes covalently attached to single-walled carbon nanotubes. | we describe the structure, activity, and stability of enzymes covalently attached to single-walled carbon nanotubes (swnts). conjugates of swnts with three functionally unrelated enzymes-horseradish peroxidase, subtilisin carlsberg, and chicken egg white lysozyme-were found to be soluble in aqueous solutions. furthermore, characterization of the secondary and tertiary structure of the immobilized proteins by circular dichroism and fluorescence spectroscopies, respectively, and determination of e ... | 2007 | 17944500 |
| actin is required for endocytic trafficking in the malaria parasite plasmodium falciparum. | the intra-erythrocytic stages of the malaria parasite endocytose large quantities of the surrounding erythrocyte cytoplasm and deliver it to a digestive food vacuole via endocytic vesicles. digestion provides amino acids for parasite protein synthesis and is required to maintain the osmotic integrity of the host cell. the parasite endocytic pathway has been described morphologically by electron microscopy, but the molecular mechanisms that mediate and regulate it remain elusive. given the involv ... | 2008 | 17944961 |
| identification of a calmodulin-binding nac protein as a transcriptional repressor in arabidopsis. | calmodulin (cam), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of a variety of proteins. however, little is known about how cam directly regulates transcription. screening of an arabidopsis cdna expression library using horseradish peroxidase-conjugated calmodulin as a probe identified a calmodulin-binding nac protein (cbnac). using gel overlay assays, a ca2+-dependent cam-binding domain was identified in the c terminus of this protein. sp ... | 2007 | 17947243 |
| the blood-testis barrier as a target of some chemotherapeutic agents. | some chemotherapeutic agents induce testicular damage, thereby affecting fertility potential. two metal-based antineoplastic complexes, titanocene dichloride and budotitane, were used to demonstrate their effects on the blood-testis barrier (btb). | 2007 | 17957098 |
| selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. | we report a method for in vitro selection of catalytically active enzymes from large libraries of variants displayed on the surface of the yeast s. cerevisiae. two libraries, each containing approximately 2 x 10(6) variants of horseradish peroxidase (hrp), were constructed; one involved error-prone pcr that sampled mutations throughout the coding sequence, whereas the other involved complete combinatorial enumeration of five positions near the active site to non-cysteine residues. the enzyme var ... | 2007 | 17961829 |
| enzymatic oxidation of aqueous pentachlorophenol. | the influence of the nonionic surfactant tween 80 on pentachlorophenol (pcp) oxidation catalyzed by horseradish peroxidase was studied. the surfactant was tested at concentrations below and above its critical micelle concentration (cmc). enhancement of pcp removal was observed at sub-cmcs. the presence of tween 80 in the reaction mixture reduced enzyme inactivation which occurred through a combination of free radical attack and sorption by precipitated products. a simple first-order model was ab ... | 2007 | 17966505 |
| [basal ganglia projections to zona incerta of dog diencephalon]. | using the method of the retrograde axonal transport of horseradish peroxidase, it was determined that in dog, the projections from globus pallidus, nucleus entopeduncularis, substantia nigra and pedunculopontine nucleus were directed to all the regions of zona incerta. in the present study, no topical features were detected in the organization of these projections in dog, since after the injections of the marker in different regions of zona incerta, similar distribution of labeled neurons was ob ... | 2007 | 17969422 |
| strong substrate-stabilizing effect of a water-miscible ionic liquid [bmim][bf(4)] in the catalysis of horseradish peroxidase. | the effects of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][bf(4)]), on both thermodynamics and kinetic mechanism of the horseradish peroxidase (hrp)-catalyzed oxidation of guaiacol (2-methoxyphenol) by h(2)o(2) were investigated. the ionic liquid stabilized the ground state of guaiacol by causing an 8-fold increase of k(m) from 3 to 23 mm upon the addition of 25% (v/v) [bmim][bf(4)]. in addition, the effect of [bmim][bf(4)] in decreasing the k(cat) value ... | 2008 | 17973090 |
| mechanism of reaction of horseradish peroxidase with chlorite and chlorine dioxide. | it is demonstrated that horseradish peroxidase (hrp) mixed with chlorite follows the whole peroxidase cycle. chlorite mediates the two-electron oxidation of ferric hrp to compound i (k(1)) thereby releasing hypochlorous acid. furthermore, chlorite acts as one-electron reductant of both compound i (k(2)) and compound ii (k(3)) forming chlorine dioxide. the strong ph-dependence of all three reactions clearly suggests that chlorous acid is the reactive species. typical apparent bimolecular rate con ... | 2008 | 17977601 |
| isolation and characterization of a novel calcium/calmodulin-dependent protein kinase, atck, from arabidopsis. | protein phosphorylation is one of the major mechanisms by which eukaryotic cells transduce extracellular signals into intracellular responses. calcium/calmodulin (ca(2+)/cam)-dependent protein phosphorylation has been implicated in various cellular processes, yet little is known about ca(2+)/cam-dependent protein kinases (camks) in plants. from an arabidopsis expression library screen using a horseradish peroxidase-conjugated soybean calmodulin isoform (scam-1) as a probe, we isolated a full-len ... | 2007 | 17978582 |
| ce-based simultaneous liquid-phase noncompetitive enzyme immunoassay for three tumor markers in human serum using electrochemical detection. | a method for indirectly detecting horseradish peroxidase (hrp) was described by ce with electrochemical detection. details of selection for optimum conditions were presented. the detection limit of free hrp was 1.09 x 10(-12) m or 0.94 zmol (s/n = 3). a novel ce-based liquid-phase binding noncompetitive enzyme immunoassay (ce-eia) was developed. in this method, after the noncompetitive immunoreaction in liquid phase, the free enzyme (hrp)-labeled antibody (ab*) and the bound enzyme-labeled compl ... | 2007 | 17979157 |
| biosensor for total cholesterol estimation using n-(2-aminoethyl)-3-aminopropyltrimethoxysilane self-assembled monolayer. | cholesterol oxidase (chox), cholesterol esterase (chet), and horseradish peroxidase (hrp) have been co-immobilized covalently on a self-assembled monolayer (sam) of n-(2-aminoethyl)-3-aminopropyltrimethoxysilane (aeapts) deposited on an indium-tin-oxide (ito) glass surface. these enzyme-modified (chox-chet-hrp/aeapts/ito) biosensing electrodes have been used to estimate cholesteryl oleate from 10 to 500 mg dl(-1). the sensitivity, km value, and shelf-life of these chet-chox-hrp/aeapts/ito biosen ... | 2007 | 17985122 |
| manganese-enhanced mri of brain plasticity in relation to functional recovery after experimental stroke. | restoration of function after stroke may be associated with structural remodeling of neuronal connections outside the infarcted area. however, the spatiotemporal profile of poststroke alterations in neuroanatomical connectivity in relation to functional recovery is still largely unknown. we performed in vivo magnetic resonance imaging (mri)-based neuronal tract tracing with manganese in combination with immunohistochemical detection of the neuronal tracer wheat-germ agglutinin horseradish peroxi ... | 2008 | 17987047 |
| electrochemical immune-biosensor for immunoglobulin g based bioelectrocatalytic reaction on micro-comb electrodes. | a new amplification strategy of electrochemical signaling from antigen-antibody interactions was proposed via back-filling immobilization of horseradish peroxidase (hrp), immunoglobulin g antibodies (anti-igg) and gold nanoparticles onto a three-dimensional sol-gel (3dsg)-functionalized biorecognition interface. the 3dsg sol-gel network was employed not only as a building block for the surface modification but also as a matrix for ligand functionalization. the signal-amplification was based on t ... | 2008 | 17987319 |
| a novel magnetic bead-based assay with high sensitivity and selectivity for analysis of telomerase in exfoliated cells from patients with bladder and colon cancer. | telomerase activity is elevated in more than 85% of cancer cells and absent in most of the normal cells and thus represents a potential cancer biomarker. we report its measurement in colon and bladder cancer cells captured using antibody-coated magnetic beads. the cells are lysed and telomerase activity is detected using a biosensor assay that employs an oligonucleotide containing the telomerase recognition sequence also covalently coupled to magnetic beads. telomerase activity is measured by th ... | 2007 | 17987629 |
| [capture and detection of human cytomegalovirus (hcmv) igm by a recombinant multi-epitope chimeric antigen]. | to establish a sensitive and specific antibody-capture enzyme-linked immunosorbent assay (ac-elisa) method to detect serum igm against human cytomegalovirus (hcmv) by expressing a recombinant hcmv multi-epitope cheimeric antigen through genetic engineering. the dominant epitopes of hcmv were analyzed and selected by computer software a recombinant multi-epitope chimeric antigen expression vector including hcmv dna was constructed, and transformed into e. coli bl21(de3). the antigen was abundantl ... | 2007 | 17989044 |
| [presence of anti hepatitis e virus antibodies in swine: is it an animal reservoir for hepatitis e?]. | swine hepatitis e virus (hev) has a cross-reaction with human anti-hev antibodies. therefore, pigs could be an animal reservoir, rendering hepatitis e as a zoonosis. the spread of this infection among infected pigs across countries would be possible through trading. previously, using an anti-human conjugate, we detected anti-hev antobodies in adult pigs in chile. | 2007 | 17989856 |
| optimization of peroxidase-catalyzed oxidative coupling process for phenol removal from wastewater using response surface methodology. | hydroxylated aromatic compounds (hacs) are considered to be primary pollutants in a wide variety of industrial wastewaters. horseradish peroxidase (hrp) is suitable for the removal of these toxic substances. however, development of a mathematical model and optimization of the hrp-based treatment considering the economical issues by novel methods is a necessity. in the present study, optimization of phenol removal from wastewater by horseradish peroxidase (hrp) was carried out using response surf ... | 2007 | 17993150 |
| a facile electrochemical method for simultaneous and on-line measurements of glucose and lactate in brain microdialysate with prussian blue as the electrocatalyst for reduction of hydrogen peroxide. | this study demonstrates a facile electrochemical method for simultaneous and selective on-line measurements of glucose and lactate in the brain of freely moving rats through integration of selective electrochemical detection with in vivo microdialysis. the selective electrochemical detection is accomplished by using oxidases as the specific sensing unit and prussian blue (pb) as the electrocatalyst for the reduction and thus for the determination of h(2)o(2) generated from the oxidase-catalyzed ... | 2007 | 17994706 |
| postsynaptic potentials and axonal projections of tegmental neurons responding to electrical stimulation of the toad striatum. | the amphibian telencephalic striatum as a major component of the basal ganglia receives multisensory information and projects to the tegmentum and other structures. however, how striatal neurons modulate tegmental activity remains unknown. here, we show by using intracellular recording and staining in toads that electrical stimulation of the ipsilateral striatum evoked an inhibitory postsynaptic potential (ipsp) in presumably binocular tegmental neurons. seventy-one neurons were intracellularly ... | 2007 | 17996369 |
| novel looped enzyme-polyamidoamine dendrimer nanohybrids used as biosensor matrix. | we report the synthesis of a novel looped enzyme-polyamidoamine nanocomposite with high enzyme loading density and long-term retention of bioactivity. the horseradish peroxidase (hrp) is first immobilized on fourth-grade (g4) poly(amidoamine) (pamam) dendrimer to form relatively a small enzyme-pamam composite, which is allowed to grow up into a larger one. the looped horseradish peroxidase-polyamidoamine (hrp-pamam) nanohybrid was characterized by tem. the material obtained shows promising featu ... | 2007 | 17996539 |
| tectorotundal connections in turtles: an electron microscopic tracing and gaba-immunocytochemical study. | the nucleus rotundus of the turtles emys orbicularis and testudo horsfieldi was analysed by axonal tracing methods and post-embedding gaba immunocytochemistry. after injections of horseradish peroxidase or biotinylated dextran amine into the optic tectum, electron microscopic observations showed that the vast majority of ipsilateral tectorotundal axon terminals were small in size, had smooth contours and contained small, round, densely packed synaptic vesicles. these terminals were gaba-immunone ... | 2007 | 17996857 |
| broccoli processing wastes as a source of peroxidase. | a peroxidase isozyme (bp) was purified to homogeneity from broccoli stems ( brassica oleraceae var. maraton) discarded from industrial processing wastes. bp specific activity was 1216 abts [2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)] units/mg, representing 466-fold that of crude extract. bp is a monomeric glycoprotein containing 16% carbohydrates, with a molecular mass of 49 kda and an isoelectric point close to 4.2. from kinetic data it showed a two-substrate ping-pong mechanism, and ... | 2007 | 17997521 |
| quadruple-analyte chemiluminometric hybridization assay. application to double quantitative competitive polymerase chain reaction. | we developed a highly sensitive quadruple-analyte chemiluminometric hybridization assay for simultaneous quantification of four nucleic acid sequences. the targets are amplified by the polymerase chain reaction (pcr) and captured to microtiter wells coated with streptavidin. the immobilized fragments are hybridized with specific probes containing a sequence complementary to the target and a sequence or a hapten that allows linkage with a chemiluminescent reporter. we prepared a mixture of four r ... | 2007 | 17997578 |
| polymer semiconductors as a biosensing platform: peroxidase activity of enzyme bound to organic semiconducting films. | organic polymer semiconductors have unique electronic properties which make them attractive for use in microelectronic and optoelectronic devices fabricated using inexpensive manufacturing processes. in addition, novel chemical and biological sensors have been proposed which make use of the photophysical and electrical properties of conjugated polymer semiconducting films. the work described herein illustrates one such biosensing application by demonstrating successful immobilization of horserad ... | 2007 | 18001900 |
| dynamin 2 mediates fluid-phase micropinocytosis in epithelial cells. | it is well-known that dynamin 2 (dyn2) participates in clathrin- and caveolae-mediated endocytosis; however, the role of dyn2 in coat-independent endocytic processes remains controversial. here we demonstrate a role for specific spliced variants of dyn2 in the micropinocytosis of fluid in epithelial cells, independent of coat-mediated endocytic pathways. a general inhibition of dyn2 was first performed using either microinjection of anti-dynamin antibodies or dyn2-sirna treatment. both of these ... | 2007 | 18003703 |
| a comparison of catalytic site intermediates of cytochrome c oxidase and peroxidases. | compounds i and ii of peroxidases such as horseradish peroxidase and cytochrome c peroxidase are relatively well understood catalytic intermediates in terms of their structures and redox states of iron, heme, and associated radical species. the intermediates involved in the oxygen reduction chemistry of the cytochrome c oxidase superfamily are more complicated because of the need for four reducing equivalents and because of the linkage of the oxygen chemistry with vectorial proton translocations ... | 2007 | 18021063 |
| dibenzothiophene oxidation by horseradish peroxidase in organic media: effect of the dbt:h2o2 molar ratio and h2o2 addition mode. | its is well known that in the biodesulfurization (bds) process the low water solubility of sulfur compounds hinders its transference from the oil phase to the cells being the rate-limiting step in the metabolism of dibenzothiophenes (dbt). thus sulfur compounds derivatives with high water solubility could be more easily transported increasing the bds efficiency. the present work performed a stepwise evaluation of the enzymatic oxidation of dbt by horseradish peroxidase (hrp). reactions were carr ... | 2008 | 18022671 |
| evaluation of enzymatic cell treatments for application of card-fish to methanogens. | several enzymatic permeabilization protocols (utilizing lysozyme, proteinase k, achromopeptidase, or recombinant pseudomurein endopeptidase [peiw]) were evaluated for application of in situ hybridization with horseradish peroxidase-labeled oligonucleotide probes and catalyzed reporter deposition (card-fish) to methanogens. in this study, twelve methanogens were selected that have typical cell surface structures: pseudomurein, surface layer, methanochondroitin and sheath. among the treatments tes ... | 2008 | 18022717 |
| evidence for corticocortical connections between areas 7 and 17 in cerebral cortex of the cat. | this study provides the first evidence of direct corticocortical connections between areas 7 and 17 of the cat. wheat germ agglutinin horseradish peroxidase (wga-hrp) was administrated by micro-electrophoresis and micro-injection, respectively, into area 17 and area 7 in different hemispheres in eight cats. wga-hrp labeled pyramidal neurons were observed primarily in layer 5 of areas 7 and 17 indicating that there are reciprocal connections between these areas. optical imaging was used to guide ... | 2008 | 18022763 |
| influence of hydrophobic and hydrophilic spacer-containing enzyme conjugates on functional parameters of steroid immunoassay. | introduction of spacers in coating steroid antigen or enzyme conjugates or immunogen is known to exert an influence on the sensitivity of steroid enzyme immunoassays. we have introduced hydrophobic and hydrophilic spacers between enzyme and steroid moieties and studied their effects on functional parameters of enzyme immunoassays, using cortisol as a model steroid. cortisol-3-o-carboxymethyloxime-bovine serum albumin (f-3-o-cmo-bsa) was used as immunogen to raise the antiserum in new zealand whi ... | 2008 | 18023401 |
| spectroscopic studies of interactions involving horseradish peroxidase and tb3+. | the spectroscopic properties of interactions involving horseradish peroxidase (hrp) and tb3+ in the simulated physiological solution was investigated with some electrochemical and spectroscopic methods, such as cyclic voltammetry (cv), circular dichroism (cd), x-ray photoelectron spectroscopy (xps) and synchronous fluorescence (sf). it was found that tb3+ can coordinate with oxygen atoms in carbonyl groups in the peptide chain of hrp, form the complex of tb3+ and hrp (tb-hrp), and then lead to t ... | 2008 | 18024195 |