Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| preferential production of g-csf by a protein-like lactobacillus rhamnosus gr-1 secretory factor through activating tlr2-dependent signaling events without activation of jnks. | different species and strains of probiotic bacteria confer distinct immunological responses on immune cells. lactobacillus rhamnosus gr-1 (gr-1) is a probiotic bacterial strain found in both the intestinal and urogenital tracts, and has immunomodulatory effects on several cell types including macrophages. however, detailed immunological responses and the signaling mechanism involved in the response are largely unknown. | 2015 | 26502905 |
| calcium phosphate supplementation increases faecal lactobacillus spp. in a randomised trial of young adults. | the aim of the studies was to determine the effects of calcium carbonate and calcium phosphate supplementation on faecal lactobacillus spp., with and without a probiotic supplement, in healthy adults. study 1 comprised of a randomised, double-blind, crossover design; participants (n=15) received 2 capsules/d of 250 mg elemental calcium as calcium carbonate (ca1) and calcium phosphate (ca2) each for 2-week periods, with 2-week baseline and washout periods. study 2 was a randomised, double-blind, ... | 2016 | 26503737 |
| lactobacillus plantarum lb95 impairs the virulence potential of gram-positive and gram-negative food-borne pathogens in ht-29 and vero cell cultures. | listeria monocytogenes, salmonella enterica and verocytotoxigenic escherichia coli (vtec) are amongst the most important agents responsible for food outbreaks occurring worldwide. in this work, two lactobacillus spp. strains (labs), lactobacillus plantarum (lb95) and lactobacillus paraplantarum (lb13), previously isolated from spontaneously fermenting olive brines, and two reference probiotic strains, lactobacillus casei shirota and lactobacillus rhamnosus gg, were investigated for their ability ... | 2016 | 26506821 |
| lactobacillus rhamnosus gg lysate increases re-epithelialization of keratinocyte scratch assays by promoting migration. | a limited number of studies have investigated the potential of probiotics to promote wound healing in the digestive tract. the aim of the current investigation was to determine whether probiotic bacteria or their extracts could be beneficial in cutaneous wound healing. a keratinocyte monolayer scratch assay was used to assess re-epithelialization; which comprises keratinocyte proliferation and migration. primary human keratinocyte monolayers were scratched then exposed to lysates of lactobacillu ... | 2015 | 26537246 |
| bugs and guts: practical applications of probiotics for gastrointestinal disorders in children. | probiotics are foods or products that contain live microorganisms that benefit the host when administered. in this clinical review, we evaluate the literature associated with using probiotics in common pediatric gastrointestinal disorders, focusing specifically on antibiotic-associated diarrhea, acute gastroenteritis, clostridium difficile infection (cdi), colic, inflammatory bowel disease, and functional gastrointestinal diseases. meta-analysis of several randomized controlled trials have confi ... | 2015 | 26538058 |
| discovery of novel small molecule modulators of clavibacter michiganensis subsp. michiganensis. | clavibacter michiganensis subsp. michiganensis (cmm) is a gram-positive seed-transmitted bacterial phytopathogen responsible for substantial economic losses by adversely affecting tomato production worldwide. a high-throughput, cell-based screen was adapted to identify novel small molecule growth inhibitors to serve as leads for future bactericide development. a library of 4,182 compounds known to be bioactive against saccharomyces cerevisiae was selected for primary screening against cmm wild-t ... | 2015 | 26539169 |
| bacillus licheniformis isolated from traditional korean food resources enhances the longevity of caenorhabditis elegans through serotonin signaling. | in this study, we investigated potentially probiotic bacillus licheniformis strains isolated from traditional korean food sources for ability to enhance longevity using the nematode caenorhabditis elegans as a simple in vivo animal model. we first investigated whether b. licheniformis strains were capable of modulating the lifespan of c. elegans. among the tested strains, preconditioning with four b. licheniformis strains significantly enhanced the longevity of c. elegans. unexpectedly, plate co ... | 2015 | 26541069 |
| probiotic strains influence on infant microbiota in the in vitro colonic fermentation model gis1. | the main goal of our study was to evaluate the effect of the individual administration of five lyophilized lactic acid bacteria strains (lactobacillus fermentum 428st, lactobacillus rhamnosus e4.2, lactobacillus plantarum fca3, lactobacillus sp. 34.1, weissella paramesenteroides ft1a) against the in vitro simulated microbiota of the human colon using the gis1 system. the influence on the metabolic activity was also assessed by quantitative determination of proteins and polysaccharides at each se ... | 2015 | 26543268 |
| potential of lactic acid bacteria at regulating escherichia coli infection and inflammation of bovine endometrium. | about 40% of dairy cattle develop uterine disease during postpartum period, causing infertility. some studies indicate that uterine infection, predominantly by escherichia coli in the first week postpartum, is associated with metritis, an uterus inflammation in which the cow fails to completely clear bacterial contaminants. the aim of this study was to evaluate the potential of four lactic acid bacteria (lab) (lactobacillus rhamnosus, pediococcus acidilactici, lactobacillus reuteri, and lactobac ... | 2016 | 26549120 |
| magnetic resonance spectroscopy reveals oral lactobacillus promotion of increases in brain gaba, n-acetyl aspartate and glutamate. | the gut microbiome has been shown to regulate the development and functions of the enteric and central nervous systems. its involvement in the regulation of behavior has attracted particular attention because of its potential translational importance in clinical disorders, however little is known about the pathways involved. we previously have demonstrated that administration of lactobacillus rhamnosus (jb-1) to healthy male balb/c mice, promotes consistent changes in gaba-a and -b receptor sub- ... | 2016 | 26577887 |
| consumption of probiotic lactobacillus rhamnosus (mtcc: 5897) containing fermented milk plays a key role in development of the immune system in newborn mice during the suckling-weaning transition. | early infancy, the period when offspring rely not only on their own immunity to combat food-borne antigens but also acquire immunity through maternal sources (via transplacental routes and breast milk), is critical for immune system development hence the present study was designed to evaluate the effect on offspring of administration of probiotic-containing fermented milk (pfm) either to mothers during the suckling period or to their offspring after weaning either separately or sequentially. pfm ... | 2016 | 26589556 |
| th17 skewing in the galt of a crohn disease patient upon lactobacillus rhamnosus gg consumption. | 2016 | 26597611 | |
| effects of fermentation with lactobacillus rhamnosus gg on product quality and fatty acids of goat milk yogurt. | the effect of fermentation with lactobacillus rhamnosus gg on the product quality of goat milk yogurt using traditional yogurt starter was studied through single-factor experiments and orthogonal experiments. the optimum fermentation condition was evaluated by the titratable acidity of goat milk yogurt, water-retaining capability, sensory score, and texture properties; the fatty acids of the fermented goat milk were determined by a gas chromatograph. results indicate that high product quality of ... | 2016 | 26601583 |
| mechanism of action of probiotic bacteria on intestinal and systemic immunities and antigen-presenting cells. | immunomodulation has been shown to be one of the major functions of probiotic bacteria. this review is presented to provide detailed information on the immunomodulatory properties of probiotics in various animal models and clinical practices. probiotics can regulate helper t (th) responses and release of cytokines in a strain-specific manner. for example, lactobacillus rhamnosus gg can induce beneficial th1 immunomodulatory effect in infants with cow's milk allergy and relieve intestinal inflamm ... | 2016 | 26606641 |
| lactobacillus rhamnosus gg supernatant promotes intestinal barrier function, balances treg and th17 cells and ameliorates hepatic injury in a mouse model of chronic-binge alcohol feeding. | impaired intestinal barrier function plays a critical role in alcohol-induced hepatic injury, and the subsequent excessive absorbed endotoxin and bacterial translocation activate the immune response that aggravates the liver injury. lactobacillus rhamnosus gg supernatant (lgg-s) has been suggested to improve intestinal barrier function and alleviate the liver injury induced by chronic and binge alcohol consumption, but the underlying mechanisms are still not clear. in this study, chronic-binge a ... | 2016 | 26617183 |
| effect of supplements: probiotics and probiotic plus honey on blood cell counts and serum iga in patients receiving pelvic radiotherapy. | radiotherapy is frequently used in treatment approaches of pelvic malignancies. nevertheless, it has some known systemic effects on blood cells and the immune system that possibly results in their susceptibility to infection. probiotics are live microbial food ingredients that provide a health advantage to the consumer. honey has prebiotic properties. the aim of this clinical trial was to investigate probable effects of probiotic or probiotics plus honey on blood cell counts and serum iga levels ... | 2015 | 26622258 |
| lactobacillus rhamnosus ccfm1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding. | lactobacillus rhamnosus ccfm1107 was screened for high antioxidative activity from 55 lactobacilli. the present study attempted to explore the protective properties of l. rhamnosus ccfm1107 in alcoholic liver injury. a mouse model was induced by orally feeding alcohol when simultaneously treated with l. rhamnosus ccfm1107, the drug hu-gan- pian (hgp), l. rhamnosus gg (lgg), and l. plantarum ccfm1112 for 3 months. biochemical analysis was performed for both serum and liver homogenate. detailed in ... | 2015 | 26626356 |
| inactivation of lactobacillus rhamnosus gg by fixation modifies its probiotic properties. | probiotics are microorganisms that have beneficial effects on the host and are safe for oral intake in a suitable dose. however, there are situations in which the administration of living microorganisms poses a risk for immunocompromised host. the objective of this study was to evaluate the influence of several fixation methods on selected biological properties of lactobacillus rhamnosus gg that are relevant to its probiotic action. fixation of the bacterial cells with ethanol, 2-propanol, gluta ... | 2016 | 26634746 |
| genomic and functional characterization of the unusual plock 0919 plasmid harboring the spacba pili cluster in lactobacillus casei lock 0919. | here, we report the extensive bioinformatic and functional analyses of the unusual plock 0919, a plasmid originating from the probiotic lactobacillus casei lock 0919 strain. this plasmid is atypical because it harbors the spacba-srtc gene cluster encoding spacba pili. we show that all other spacba-srtc sequences of the lactobacillus genus that have been previously described and deposited in genbank are present in the chromosomal dna. another important observation for plock 0919 is that the spacb ... | 2015 | 26637469 |
| wastes from bioethanol and beer productions as substrates for l(+) lactic acid production - a comparative study. | waste substrates from bioethanol and beer productions are cheap, abundant and renewable substrates for biorefinery production of lactic acid (la) and variability in their chemical composition presents a challenge in their valorisation. three types of waste substrates, wasted bread and wasted potato stillage from bioethanol production and brewers' spent grain hydrolysate from beer production were studied as substrates for the production of l(+) la and probiotic biomass by lactobacillus rhamnosus ... | 2016 | 26639411 |
| a novel consortium of lactobacillus rhamnosus and streptococcus thermophilus for increased access to functional fermented foods. | the lactic acid bacterium lactobacillus rhamnosus gg is the most studied probiotic bacterium with proven health benefits upon oral intake, including the alleviation of diarrhea. the mission of the yoba for life foundation is to provide impoverished communities in africa increased access to lactobacillus rhamnosus gg under the name lactobacillus rhamnosus yoba 2012, world's first generic probiotic strain. we have been able to overcome the strain's limitations to grow in food matrices like milk, b ... | 2015 | 26643044 |
| efficacy of probiotic use in acute rotavirus diarrhea in children: a systematic review and meta-analysis. | probiotic therapies with different strains demonstrated some beneficial effects, although some studies did not show any significant effects. this study assessed systematically the current knowledge on the effect of probiotic bacteria on duration of acute rotavirus diarrhea in children compared with control. | 2015 | 26644891 |
| lactobacillus rhamnosus gg supplementation during critical windows of gestation influences immune phenotype in swiss albino mice offspring. | probiotic supplementation during critical windows of gestation might have a significant influence on the infant's immune phenotype. swiss albino mice (f0 generation) aged 31 days were supplemented orally with probiotic lactobacillus rhamnosus gg (lgg); and the supplementation was continued throughout mating, gestation and lactation. the pups (f1 generation) born to them were separated post weaning and received either the same probiotic supplementation as their mothers or were denied supplementat ... | 2016 | 26645351 |
| specific probiotic dietary supplementation leads to different effects during remission and relapse in murine chronic colitis. | although interest in using probiotics to prevent and treat intestinal diseases is increasing, the effects of specific probiotic strains still remain unclear. here, we assess the therapeutic effects of two probiotic strains, lactobacillus rhamnosus nutres 1 and bifidobacterium breve nutres 204 on a dextran sodium sulphate (dss)-induced chronic murine colitis model. the chronic colitis was induced by two dss treatment cycles with a rest period of 10 days (the remission or resolution phase). the pr ... | 2016 | 26645352 |
| assessment and comparison of probiotic potential of four lactobacillus species isolated from feces samples of iranian infants. | the probiotic potential of lactobacillus species isolated from infant feces was investigated. for this study, the antibiotic susceptibility, tolerance in gut-related conditions, antimicrobial activity, and ability to adhere to a human colorectal adenocarcinoma cell line (caco-2 cells) of four common lactobacillus species (lactobacillus paracasei [n = 15], lactobacillus rhamnosus [n = 45], lactobacillus gasseri [n = 20] and lactobacillus fermentum [n = 18]) were assessed. most isolates that which ... | 2016 | 26648174 |
| relative cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic lactobacillus rhamnosus gg in managing infants with cow's milk allergy in spain. | to estimate the cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic lactobacillus rhamnosus gg (ehcf + lgg; nutramigen lgg) as a first-line management for cow's milk allergy compared with ehcf alone, and amino acid formulae in spain, from the perspective of the spanish national health service (sns). | 2015 | 26648744 |
| live and heat-killed lactobacillus rhamnosus atcc 7469 may induce modulatory cytokines profiles on macrophages raw 264.7. | this study aimed to evaluate the capacity of lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (tnf-α, il-1β, il-4, il-6, il-10, and il-12) by mouse macrophages (raw 264.7). three microorganism preparations were used: live l. rhamnosus (llr) suspension, heat-killed l. rhamnosus (hklr) suspension, and the supernatant of a heat-killed l. rhamnosus (shklr) suspension, which were cultured with macrophages (37°c, 5% co2) for 2 h and 30 min. after that, cells were cultur ... | 2015 | 26649329 |
| lactobacillus rhamnosus gr-1 ameliorates escherichia coli-induced inflammation and cell damage via attenuation of asc-independent nlrp3 inflammasome activation. | escherichia coli is a major environmental pathogen causing bovine mastitis, which leads to mammary tissue damage and cell death. we explored the effects of the probiotic lactobacillus rhamnosus gr-1 on ameliorating e. coli-induced inflammation and cell damage in primary bovine mammary epithelial cells (bmecs). increased toll-like receptor 4 (tlr4), nod1, and nod2 mrna expression was observed following e. coli challenge, but this increase was attenuated by l. rhamnosus gr-1 pretreatment. immunofl ... | 2015 | 26655757 |
| microbiological characteristics of the probiotic strains b. longum bb536 and l. rhamnosus hn001 used in combination. | the aim of this study was to investigate the antagonistic activity between the probiotic strains bifidobacterium longum (b. longum) bb536 and lactobacillus rhamnosus (l. rhamnosus) hn001 (zircombi, alfa wassermann s.p.a., italy) and to evaluate for the strains tested alone and in combination the resistance in simulated gastrointestinal conditions, the ability to adhere to epithelial intestinal cells and their competition for adhesion. | 2015 | 26657925 |
| lactic acid bacteria protect human intestinal epithelial cells from staphylococcus aureus and pseudomonas aeruginosa infections. | staphylococcus aureus and pseudomonas aeruginosa are opportunistic pathogens that cause nosocomial and food-borne infections. they promote intestinal diseases. gastrointestinal colonization by s. aureus and p. aeruginosa has rarely been researched. these organisms spread to extra gastrointestinal niches, resulting in increasingly progressive infections. lactic acid bacteria are gram-positive bacteria that produce lactic acid as the major end-product of carbohydrate fermentation. these bacteria i ... | 2015 | 26681052 |
| lactobacillus and leuconostoc volatilomes in cheese conditions. | new strains are desirable to diversify flavour of fermented dairy products. the objective of this study was to evaluate the potential of leuconostoc spp. and lactobacillus spp. in the production of aroma compounds by metabolic fingerprints of volatiles. eighteen strains, including five lactobacillus species (lactobacillus fermentum, lactobacillus helveticus, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus sakei) and three leuconostoc species (leuconostoc citreum, leuconostoc lact ... | 2016 | 26685674 |
| absence of adverse events in healthy individuals using probiotics--analysis of six randomised studies by one study group. | consumption of live bacteria as probiotic supplements is increasing. there is, however, a lack of information on the safety of ingested probiotics. the main objective of this study was to investigate the adverse events (aes) of specific probiotics (lactobacillus rhamnosus gg (lgg) alone or lgg in combination with l. rhamnosus lc705, propionibacterium freudenreichii js, bifidobacterium lactis bb12, or bifidobacterium breve 99) studied in six of our study groups' clinical trials, by analysing indi ... | 2016 | 26689224 |
| probiotics for the prevention of pediatric antibiotic-associated diarrhea. | antibiotics are frequently prescribed in children. they alter the microbial balance within the gastrointestinal tract, commonly resulting in antibiotic-associated diarrhea (aad). probiotics may prevent aad via restoration of the gut microflora. | 2015 | 26695080 |
| human gut-commensalic lactobacillus ruminis atcc 25644 displays sortase-assembled surface piliation: phenotypic characterization of its fimbrial operon through in silico predictive analysis and recombinant expression in lactococcus lactis. | sortase-dependent surface pili (or fimbriae) in gram-positive bacteria are well documented as a key virulence factor for certain harmful opportunistic pathogens. however, it is only recently known that these multi-subunit protein appendages are also belonging to the "friendly" commensals and now, with this new perspective, they have come to be categorized as a niche-adaptation factor as well. in this regard, it was shown earlier that sortase-assembled piliation is a native fixture of two human i ... | 2015 | 26709916 |
| compositional and physicochemical factors governing the viability of lactobacillus rhamnosus gg embedded in starch-protein based edible films. | probiotic incorporation in edible films and coatings has been shown recently to be an efficient strategy for the delivery of probiotics in foods. in the present work, the impact of the compositional, physicochemical and structural properties of binary starch-protein edible films on lactobacillus rhamnosus gg viability and stability was evaluated. native rice and corn starch, as well as bovine skin gelatine, sodium caseinate and soy protein concentrate were used for the fabrication of the probiot ... | 2016 | 26726280 |
| dietary lactobacillus rhamnosus gg supplementation improves the mucosal barrier function in the intestine of weaned piglets challenged by porcine rotavirus. | lactobacillus rhamnosus gg (lgg) has been regarded as a safe probiotic strain. the aim of this study was to investigate whether dietary lgg supplementation could alleviate diarrhea via improving jejunal mucosal barrier function in the weaned piglets challenged by rv, and further analyze the potential roles for apoptosis of jejunal mucosal cells and intestinal microbiota. a total of 24 crossbred barrows weaned at 21 d of age were assigned randomly to 1 of 2 diets: the basal diet and lgg supplemen ... | 2016 | 26727003 |
| probiotic treatment reduces appetite and glucose level in the zebrafish model. | the gut microbiota regulates metabolic pathways that modulate the physiological state of hunger or satiety. nutrients in the gut stimulate the release of several appetite modulators acting at central and peripheral levels to mediate appetite and glucose metabolism. after an eight-day exposure of zebrafish larvae to probiotic lactobacillus rhamnosus, high-throughput sequence analysis evidenced the ability of the probiotic to modulate the microbial composition of the gastrointestinal tract. these ... | 2016 | 26727958 |
| three novel candidate probiotic strains with prophylactic properties in a murine model of cow's milk allergy. | food allergies can have significant effects on morbidity and on quality of life. therefore, the development of efficient approaches to reduce the risk of developing food allergies is of considerable interest. the aim of this study was to identify and select probiotic strains with preventive properties against allergies using a combination of in vitro and in vivo approaches. to that end, 31 strains of bifidobacteria and lactic acid bacteria were screened for their immunomodulatory properties in t ... | 2016 | 26729723 |
| the efficacy of probiotic (lactobacillus rhamnosus gg) and 5-asa (aminosalicylic acid) in the treatment of experimental radiation proctitis in rats. | the aims of the study are to demonstrate the effect of probiotic use on the healing of radiation proctitis (rp) and evaluate the efficiency of fecal biomarkers at follow-up of the treatment. thirty-two male/female rats were randomly separated into four groups of eight rats. the first group (control) was not radiated. rp was created by 17.5 gy single dose rectal irradiation. the second group (rp) was subjected to rp, but not treated. the third group (rp+asa) was treated with 5-aminosalicylic acid ... | 2015 | 26730065 |
| crystallization and x-ray crystallographic analysis of the adhesive spac pilin subunit in the spacba pilus of gut-adapted lactobacillus rhamnosus gg. | gram-positive lactobacillus rhamnosus gg, a gut-adapted commensalic (and probiotic) strain, is known to express sortase-assembled pili on its cell surface. these spacba-called pili consist of three different types of building blocks; the spaa backbone-pilin subunit and the spab and spac ancillary pilins. spac is a relatively large (~90kda) multi-domain fimbrial adhesin, and while it is located primarily at the spacba pilus tip, occasionally, it can also be detected throughout the length of pilus ... | 2016 | 26732247 |
| evaluation of efficacy and safety of lactobacillus rhamnosus in children aged 4-48 months with atopic dermatitis: an 8-week, double-blind, randomized, placebo-controlled study. | the main objective of this study was to evaluate the efficacy and safety of lactobacillus rhamnosus in children aged 4-48 months with atopic dermatitis. | 2015 | 26733351 |
| the expression of heterologous mam-7 in lactobacillus rhamnosus reduces its intrinsic capacity to inhibit colonization of pathogen vibrio parahaemolyticus in vitro. | vibrio parahaemolyticus (v. parahaemolyticus) is a gram-negative, halophilic bacterium recognized as one of the most important foodborne pathogen. when ingested, v. parahaemolyticus causes a self-limiting illness (vibriosis), characterized mainly by watery diarrhoea. treatment is usually oral rehydration and/or antibiotics in complicated cases. since 1996, the pathogenic and pandemic v. parahaemolyticus o3:k6 serotype has spread worldwide, increasing the reported number of vibriosis cases. thus, ... | 2016 | 26739707 |
| novel method based on chromogenic media for discrimination and selective enumeration of lactic acid bacteria in fermented milk products. | microbial analyses of fermented milk products require selective methods to discriminate between close species simultaneously present in high amounts. a culture-based method combining novel chromogenic agar media and appropriate incubation conditions was developed to enumerate lactic acid bacteria (lab) strains in fermented milk. m1 agar, containing two chromogenic substrates, allowed selective enumeration of lactobacillus rhamnosus, two strains of lactobacillus paracasei subsp. paracasei and str ... | 2016 | 26742619 |
| probiotic compared with standard milk for high-caries children: a cluster randomized trial. | the aim of this study was to compare milk supplemented with probiotic lactobacilli with standard milk for the increment of caries in preschool children after 10 mo of intervention. the study was a triple-blind, placebo-controlled randomized trial. participants were children aged 2 and 3 y (n = 261) attending 16 nursery schools in a metropolitan region in chile. nursery schools were randomly assigned to 2 parallel groups: children in the intervention group were given 150 ml of milk supplemented w ... | 2016 | 26747421 |
| optimization performance of an ansbbr applied to biohydrogen production treating whey. | the present study investigated the influence of the influent concentration of substrate, feeding time and temperature on the production of biohydrogen from cheese whey in an ansbbr with liquid phase recirculation. the highest hydrogen yield (0.80 molh2.mollactose(-1)) and productivity (660 mlh2 l(-1) d(-1)) were achieved for influent concentrations of 5400 mgdqo l(-1). no significant difference was noted in the biological hydrogen production for the feeding time conditions analyzed. the lowest t ... | 2016 | 26751813 |
| probiotics for the prevention of antibiotic-associated diarrhea in children. | this article provides recommendations, developed by the working group (wg) on probiotics of the european society for pediatric gastroenterology, hepatology, and nutrition, for the use of probiotics for the prevention of antibiotic-associated diarrhea (aad) in children based on a systematic review of previously completed systematic reviews and of randomized controlled trials published subsequently to these reviews. the use of probiotics for the treatment of aad is not covered. the recommendations ... | 2016 | 26756877 |
| antilisterial bacteriocin from lactobacillus rhamnosus cjnu 0519 presenting a narrow antimicrobial spectrum. | a lactic acid bacterium presenting antimicrobial activity against a lactobacillus acidophilus strain used for eradication of acid inhibition was isolated from a natural cheese. the 16s rrna gene sequence of the isolate best matched with a strain of l. rhamnosus and was designated l. rhamnosus cjnu 0519. the antimicrobial activity of the partially purified bacteriocin of cjnu 0519 was abolished when treated with a protease, indicating the protein nature of the bacteriocin. the partially purified ... | 2015 | 26761811 |
| effects of dietary supplementation of lactobacillus rhamnosus or/and lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, pagrus major. | pagrus major fingerlings (3·29 ± 0·02 g) were fed with basal diet (control) supplemented with lactobacillus rhamnosus (lr), lactococcus lactis (ll), and l. rhamnosus + l. lactis (lr + ll) at 10(6) cell g(-1) feed for 56 days. feeding a mixture of lr and ll significantly increased feed utilization (fer and per), intestine lactic acid bacteria (lab) count, plasma total protein, alternative complement pathway (acp), peroxidase, and mucus secretion compared with the other groups (p < 0.05). serum ly ... | 2016 | 26766177 |
| lactobacillus sepsis following a laparotomy in a preterm infant: a note of caution. | probiotics for preterm infants have been shown to reduce the incidence of necrotising enterocolitis and all-cause mortality in a recent meta-analysis. it has been argued, however, that some of these results may not be applicable to specific subgroups, e.g. infants with a birth weight of <1,000 g. the specific role of probiotics in improving health outcomes in preterm and term infants following intestinal surgery is not well defined. we report a case of a premature infant diagnosed with late-onse ... | 2016 | 26780534 |
| peptide extracts from cultures of certain lactobacilli inhibit helicobacter pylori. | helicobacter pylori inhibition by probiotic lactobacilli has been observed in vitro and in vivo. carefully selected probiotic lactobacillus strains could therefore play an important role in the treatment of h. pylori infection and eradication. however, the underlying mechanism for this inhibition is not clear. the aim of this study was to examine if peptide extracts, containing bacteriocins or other antibacterial peptides, from six lactobacillus cultures (lactobacillus acidophilus la1, lactobaci ... | 2010 | 26780898 |
| lactobacillus rhamnosus bfe 5264 and lactobacillus plantarum nr74 promote cholesterol excretion through the up-regulation of abcg5/8 in caco-2 cells. | the effect of two putative probiotic strains, lactobacillus rhamnosus bfe5264 and lactobacillus plantarum nr74, on the control of cholesterol efflux in enterocytes was assessed by focusing on the promotion of atp-binding cassette sub-family g members 5 and 8 (abcg5 and abcg8). differentiated caco-2 enterocytes were treated with live bacteria, heat-killed bacteria, a bacterial cell wall fraction, and metabolites and were subjected to cholesterol uptake assay, mrna analysis, and protein analyses. ... | 2011 | 26781680 |
| quantitative appraisal of the probiotic attributes and in vitro adhesion potential of anti-listerial bacteriocin-producing lactic acid bacteria. | estimation of bile tolerance, endurance to gastric and intestinal environment and adhesion potential to intestinal cells are significant selection criteria for probiotic lactic acid bacteria (lab). in this paper, the probiotic potential of native bacteriocin-producing lab isolated previously from indigenous source has been determined through quantitative approaches. among fifteen anti-listerial bacteriocin-producing native lab, ten strains were found to be bile tolerant. the presence of bile sal ... | 2013 | 26782735 |
| detection and specific enumeration of multi-strain probiotics in the lumen contents and mucus layers of the rat intestine after oral administration. | although the detection of viable probiotic bacteria following their ingestion and passage through the gastrointestinal tract (git) has been well documented, their mucosal attachment in vivo is more difficult to assess. in this study, we investigated the survival and mucosal attachment of multi-strain probiotics transiting the rat git. rats were administered a commercial mixture of the intestinal probiotics lactobacillus acidophilus la742, lactobacillus rhamnosus l2h and bifidobacterium lactis hn ... | 2009 | 26783165 |
| development of antioxidant rich fruit supplemented probiotic yogurts using free and microencapsulated lactobacillus rhamnosus culture. | the present study reports the preparation of probiotic yogurt using lactobacillus rhamnosus. the standard starter cultures used for yogurt fermentation were streptococcus thermophilus and lactobacillus delbrueckii subsp. bulgaricus and obtained from ndri, karnal, india. the prepared yogurt was supplemented with fruit pulp (10 % w/v) of apricot, raspberries, plum and jamun. these fruits were rich in antioxidant property as observed by dpph, nitric oxide radical scavenging and ferric reducing anti ... | 2016 | 26787986 |
| differential effects of escherichia coli nissle and lactobacillus rhamnosus strain gg on human rotavirus binding, infection, and b cell immunity. | rotavirus (rv) causes significant morbidity and mortality in children worldwide. the intestinal microbiota plays an important role in modulating host-pathogen interactions, but little is known about the impact of commonly used probiotics on human rv (hrv) infection. in this study, we compared the immunomodulatory effects of gram-positive (lactobacillus rhamnosus strain gg [lgg]) and gram-negative (escherichia coli nissle [ecn]) probiotic bacteria on virulent human rotavirus (virhrv) infection an ... | 2016 | 26800875 |
| immobilization of lactobacillus rhamnosus in mesoporous silica-based material: an efficiency continuous cell-recycle fermentation system for lactic acid production. | lactic acid bacteria immobilization methods have been widely used for lactic acid production. until now, the most common immobilization matrix used is calcium alginate. however, ca-alginate gel disintegrated during lactic acid fermentation. to overcome this deficiency, we developed an immobilization method in which lactobacillus rhamnosus cells were successfully encapsulated into an ordered mesoporous silica-based material under mild conditions with a high immobilization efficiency of 78.77% by ... | 2016 | 26803707 |
| isolation, identification, and evaluation of novel probiotic strains isolated from feces of breast-fed infants. | to isolate, identify, and evaluate the probiotic properties of lactic acid bacteria (lab) isolated from the feces of breast-fed infants. | 2016 | 26817236 |
| prevalence of probiotic use among inpatients: a descriptive study of 145 u.s. hospitals. | to inform clinical guidance, public health efforts, and research directions, probiotic use in u.s. health care needs to be better understood. this work aimed to assess the prevalence of inpatient probiotic use in a sample of u.s. hospitals. | 2016 | 26822808 |
| effect of fermented broth from lactic acid bacteria on pathogenic bacteria proliferation. | in this study, the effect that 5 fermented broths of lactic acid bacteria (lab) strains have on the viability or proliferation and adhesion of 7 potentially pathogenic microorganisms was tested. the fermented broth from lactococcus lactis c660 had a growth inhibitory effect on escherichia coli k92 that reached of 31%, 19% to pseudomonas fluorescens, and 76% to staphylococcus epidermidis. the growth of staph. epidermidis was negatively affected to 90% by lc. lactis 11454 broth, whereas the growth ... | 2016 | 26851857 |
| effects of prebiotic oligosaccharides consumption on the growth and expression profile of cell surface-associated proteins of a potential probiotic lactobacillus rhamnosus fsmm15. | to investigate carbohydrate preference of a potential probiotic, lactobacillus rhamnosus fsmm15, six prebiotics, including two milk-derived prebiotics, galactooligosaccharides and lacto-n-biose i, and four plant-origin prebiotics, beet oligosaccharide syrup, difructose anhydride iii, fructooligosaccharides, and raffinose, were examined. the strain utilized the milk-derived prebiotics at similar levels to glucose but did not utilize the plant-origin ones in the same manner, reflecting their genet ... | 2016 | 26858929 |
| transcriptomic profile of whole blood cells from elderly subjects fed probiotic bacteria lactobacillus rhamnosus gg atcc 53103 (lgg) in a phase i open label study. | we examined gene expression of whole blood cells (wbc) from 11 healthy elderly volunteers participating on a phase i open label study before and after oral treatment with lactobacillus rhamnosus gg-atcc 53103 (lgg)) using rna-sequencing (rna-seq). elderly patients (65-80 yrs) completed a clinical assessment for health status and had blood drawn for cellular rna extraction at study admission (baseline), after 28 days of daily lgg treatment (day 28) and at the end of the study (day 56) after lgg t ... | 2016 | 26859761 |
| lactobacillus rhamnosus gg effect on behavior of zebrafish during chronic ethanol exposure. | ethanol is a widely consumed drug, which acts on the central nervous system to induce behavioral alterations ranging from disinhibition to sedation. recent studies have produced accumulating evidence for the therapeutic role of probiotic bacteria in behavior. we aimed to investigate the effect of lactobacillus rhamnosus gg (lgg) on the behavior of adult zebrafish chronically exposed to ethanol. adult wild-type zebrafish were randomly divided into four groups, each containing 15 fish. the followi ... | 2016 | 26862467 |
| the effect of lactobacillus crispatus and lactobacillus rhamnosusculture supernatants on expression of autophagy genes and hpv e6 and e7 oncogenes in the hela cell line. | the aim of this study was to clarify the mechanism by which lactobacilli exert their cytotoxic effects on cervical cancer cells. in addition, we aimed to evalu- ate the effect of lactobacilli on the expression of human papilloma virus (hpv) onco- genes. | 2016 | 26862519 |
| isoflavone metabolism by a collection of lactic acid bacteria and bifidobacteria with biotechnological interest. | almost all soy isoflavones exist as glycosides, daidzin, genistin, and glycitin. we analyzed the capacity of 92 strains of lactic acid bacteria (lab) and bifidobacteria with biotechnological interest to process the glycosylated isoflavones daidzin, genistin, and glycitin in their more bioavailable aglycones and their metabolites as dihydrodaidzein (dhd), o-desmethylangolensin, and equol. representative strains of the four genera studied lactobacillus, enterococcus, lactococcus, and bifidobacteri ... | 2016 | 26878882 |
| maximum-biomass prediction of homofermentative lactobacillus. | fed-batch and ph-controlled cultures have been widely used for industrial production of probiotics. the aim of this study was to systematically investigate the relationship between the maximum biomass of different homofermentative lactobacillus and lactate accumulation, and to develop a prediction equation for the maximum biomass concentration in such cultures. the accumulation of the end products and the depletion of nutrients by various strains were evaluated. in addition, the minimum inhibito ... | 2016 | 26896862 |
| hypoallergenic formula with lactobacillus rhamnosus gg for babies with colic: a pilot study of recruitment, retention, and fecal biomarkers. | to investigate recruitment, retention, and estimates for effects of formula supplementation with lactobacillus rhamnosus gg (lgg) on inflammatory biomarkers and fecal microbial community in infants with colic. | 2016 | 26909239 |
| supplementation of xylitol-containing chewing gum with probiotics: a double blind, randomised pilot study focusing on saliva flow and saliva properties. | the aim of this study was to investigate the impact of daily chewing, for 12 weeks, of 2 different probiotic gums compared with placebo on saliva flow rate, saliva iga levels and saliva ph. the intervention study included 54 adult volunteers with hyposalivation in a double-blind, randomised and placebo-controlled design with three parallel groups. volunteers were randomly assigned to 3 different groups: subjects in group a (n = 19) were given placebo chewing gum, group b (n = 17) received bifido ... | 2016 | 26913493 |
| bifidobacterium lactis 420 and fish oil enhance intestinal epithelial integrity in caco-2 cells. | increased intestinal permeability is a predisposing factor for low-grade inflammation-associated conditions, including obesity and type 2 diabetes. dietary components may influence intestinal barrier integrity. we hypothesized that the dietary supplements bifidobacterium lactis 420, lactobacillus rhamnosus hn001, and fish oil have beneficial impacts on intestinal barrier integrity. in addition, we hypothesized that the coadministration of these components results in synergistic benefits to the i ... | 2016 | 26923511 |
| chlamydia pneumoniae enhances the th2 profile of stimulated peripheral blood mononuclear cells from asthmatic patients. | chlamydia pneumoniae is a cause of respiratory infection in adults and children. there is evidence for an association between atypical bacterial respiratory pathogens and the pathogenesis of asthma. we compared t helper (th) responses in c. pneumoniae - infected peripheral blood mononuclear cells (pbmc) in patients with or without asthma. pbmc (1×10(6)/ml) from asthmatic patients (n=11) and non-asthmatic controls (n=12) were infected or mock-infected for 1h +/- c. pneumoniae tw-183 at a multipli ... | 2016 | 26924667 |
| immunological mechanisms involved in probiotic-mediated protection against citrobacter rodentium-induced colitis. | inflammatory bowel disease is a group of chronic, incurable inflammatory disorders of the gastrointestinal tract that cause severe diarrhoea, intestinal inflammation, pain, fatigue and weight loss. in this study, we first developed a model of citrobacter rodentium-induced colitis and then evaluated the protective effects of selected probiotics on inflammation. the results showed that administration of a combination of probiotics including lactobacillus rhamnosus atcc 53103, lactobacillus acidoph ... | 2016 | 26925601 |
| inhibition of enteropathogens adhesion to human enterocyte-like ht-29 cells by a dairy strain of propionibacterium acidipropionici. | adhesion to the host intestinal mucosa is considered relevant for orally delivered probiotics as it prolongs their persistence in the gut and their health promoting effects. classical propionibacteria are microorganisms of interest due to their role as dairy starters as well as for their functions as probiotics. propionibacterium acidipropionici q4, is a dairy strain isolated from a swiss-type cheese made in argentina that displays probiotic potential. in the present work we assessed the ability ... | 2016 | 26925604 |
| isolation and characterization of lactobacilli from human faeces and indigenous fermented foods for their potential application as probiotics. | this study was conducted to select lactobacillus strains from various sources on the basis of their probiotic attributes, such as acid and bile tolerance, binding to intestinal cells, and antimicrobial activity. twelve isolates were obtained from human and food sources and were evaluated against standard probiotic lactobacillus rhamnosus gg (lgg). isolates were also studied for their antibiotic susceptibility. isolate lactobacillus fermentum gpi-6 showed the best survival profile at 0.3% and 1% ... | 2016 | 26928011 |
| lactobacillus sepsis and probiotic therapy in newborns: two new cases and literature review. | many term and preterm infants are commonly supplemented with probiotics to prevent adverse effects of antibiotic administration and necrotizing enterocolitis and they are believed to be safe. however, the supplementation with lactobacillus rhamnosus gg has been associated with the development of sepsis with a cause-effect relationship in six newborns and children. in this study, we report two further cases and discuss the emerging issue of probiotic supplementation safety in neonates. we conclud ... | 2016 | 26929865 |
| clinical effects of lactobacillus rhamnosus in non-surgical treatment of chronic periodontitis: a randomized placebo-controlled trial with 1-year follow-up. | probiotics are living microorganisms that provide beneficial effects for the host when administered in proper quantities. the aim of this double-masked placebo-controlled parallel-arm randomized clinical trial is to evaluate the clinical effects of a lactobacillus rhamnosus sp1-containing probiotic sachet as an adjunct to non-surgical therapy. | 2016 | 26944407 |
| immunomodulation of lactobacillus rhamnosus gg (lgg)-derived soluble factors on antigen-presenting cells of healthy blood donors. | lactobacillus rhamnosus gg (lgg) cells have been shown to promote type-1 immune responsiveness; however knowledge of immunomodulation of soluble factors secreted by lgg is limited. this is the first study to investigate whether lgg soluble factors promote a comparable immune responsiveness as the bacterial cells. both treatments - lgg conditioned medium with (cm + lgg) or without (cm) lgg cells, in this study increased expression of several toll-like receptors (tlrs) in all studied cell types an ... | 2016 | 26961406 |
| secretory iga in complex with lactobacillus rhamnosus potentiates mucosal dendritic cell-mediated treg cell differentiation via tlr regulatory proteins, raldh2 and secretion of il-10 and tgf-β. | the importance of secretory iga in controlling the microbiota is well known, yet how the antibody affects the perception of the commensals by the local immune system is still poorly defined. we have previously shown that the transport of secretory iga in complex with bacteria across intestinal microfold cells results in an association with dendritic cells in peyer's patches. however, the consequences of such an interaction on dendritic cell conditioning have not been elucidated. in this study, w ... | 2016 | 26972771 |
| probiotic gut microbiota isolate interacts with dendritic cells via glycosylated heterotrimeric pili. | mapping of the microbial molecules underlying microbiota-host interactions is key to understand how microbiota preserve mucosal homeostasis. a pivotal family of such bacterial molecules are pili. pili are proteinaceous cell wall appendages with a well-documented role in adhesion, whilst their role in immune interaction with the host is less established. gram-positive pili are often posttranslationally modified by sortase-specific cleavage reactions and the formation of intramolecular peptide bon ... | 2016 | 26985831 |
| human rhinovirus in experimental infection after peroral lactobacillus rhamnosus gg consumption, a pilot study. | data has emerged on possible beneficial effects of probiotics in respiratory tract viral infections, but it is unclear if the promising positive effects evidenced are due to a reduced viral load during infections. the aims of this work were to investigate the effect of peroral probiotic lactobacillus rhamnosus gg (american type culture collection [atcc], accession no. 53103) consumption on human rhinovirus (hrv) load in nasopharyngeal lavage samples in experimental hrv infection, and to correlat ... | 2016 | 26990147 |
| lactobacillus rhamnosus could inhibit porphyromonas gingivalis derived cxcl8 attenuation. | an increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. p. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits cxcl8 expression. | 2016 | 27008259 |
| whole-cell biocatalysis for producing ginsenoside rd from rb1 using lactobacillus rhamnosus gg. | ginsenosides are the major active ingredients in ginseng used for human therapeutic plant medicines. one of the most well-known probiotic bacteria among the various strains on the functional food market is lactobacillus rhamnosus gg. biocatalytic methods using probiotic enzymes for producing deglycosylated ginsenosides such as rd have a growing significance in the functional food industry. the addition of 2% cellobiose (w/v) to glucose-free de man-rogosa-sharpe broths notably induced β-glucosida ... | 2016 | 27012233 |
| fermented milk with probiotic lactobacillus rhamnosus s1k3 (mtcc5957) protects mice from salmonella by enhancing immune and nonimmune protection mechanisms at intestinal mucosal level. | we investigated the mechanism by which an indian indigenous probiotic culture, lactobacillus rhamnosus s1k3, could overcome the pathogenic strain salmonella enterica with an emphasis on the response at the intestinal mucosal level after long-term (30days) consumption. s1k3 was able to produce antimicrobial compounds against the pathogens. the probiotic adhered strongly to intestinal epithelium and maintained its integrity in presence of salmonella through stimulation of tight junction and antimi ... | 2016 | 27012622 |
| the maternal control in the embryonic development of zebrafish. | the maternal control directing the very first hours of life is of pivotal importance for ensuring proper development to the growing embryo. thanks to the finely regulated inheritance of maternal factors including mrnas and proteins produced during oogenesis and stored into the mature oocyte, the embryo is sustained throughout the so-called maternal-to-zygotic transition, a period in development characterized by a species-specific length in time, during which critical biological changes regarding ... | 2017 | 27013380 |
| lactobacillus rhamnosus gg increases toll-like receptor 3 gene expression in murine small intestine ex vivo and in vivo. | administration of lactobacillus rhamnosus gg (lgg) has been reported to be therapeutically effective against acute secretory diarrhoea resulting from the structural and functional intestinal mucosal lesions induced by rotavirus infection; however, the underlying mechanisms remain to be completely elucidated. because toll-like receptor 3 (tlr3) plays a key role in the innate immune responses following the recognition of rotavirus, the present study examined whether lgg influences tlr3 gene expres ... | 2016 | 27013459 |
| protective effects of lactobacillus rhamnosus gg against dyslipidemia in high-fat diet-induced obese mice. | recent reports suggest that gut microbiota can be a major determinant of dyslipidemia and non-alcoholic fatty liver disease (nafld) and its modulation by treating probiotics is a valid strategy to exert a protective effect. in this study, high-fat diet (hfd)-fed mice were orally administrated with lactobacillus rhamnosus gg (lgg) for 13 weeks. significant reductions in the weights of the liver, mesenteric and subcutaneous adipose tissues were observed in lgg-treated hfd-fed mice compared to lgg- ... | 2016 | 27018382 |
| [susceptibility of lactobacillus rhamnosus strains isolated from probiotic products to antimicrobial agents]. | probiotics generally recognized as safe (gras), when given in relevant dose, are able to induce strain-specific beneficial effects for health of humans or animals. | 2015 | 27019914 |
| improvement of l-lactic acid productivity from sweet sorghum juice by repeated batch fermentation coupled with membrane separation. | in order to efficiently produce l-lactic acid from non-food feedstocks, sweet sorghum juice (ssj), which is rich of fermentable sugars, was directly used for l-lactic acid fermentation by lactobacillus rhamnosus la-04-1. a membrane integrated repeated batch fermentation (mirb) was developed for productivity improvement. high-cell-density fermentation was achieved with a final cell density (od620) of 42.3, and the ccr effect was overcomed. when ssj (6.77gl(-1) glucose, 4.51gl(-1) fructose and 50. ... | 2016 | 27023384 |
| restoration gaps needed to exceed a threshold size to impede sealed lesion arrest in vitro. | after selective excavation, bacteria are sealed beneath restorations. leaking restorations could maintain carbohydrate-supply, leading to lesion progression and pulp damage. it is unclear if fluid (and thereby carbohydrate) exchange occurs through any interfacial gaps, or if such exchange only occurs in case gaps exceed a certain threshold size. we investigated how different restoration gap sizes impact on survival of sealed bacterial in vitro. | 2016 | 27026078 |
| probiotic bacterial and fungal strains: claims with evidence. | probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit for the host. this review summarizes current (i.e. published in the last 5 years) key evidence on probiotic efficacy and its safety in adults and children. to identify relevant data, searches of medline and the cochrane library databases were performed in august 2015 to locate randomized controlled trials (rcts) or their meta-analyses. the focus was on commonly used, well-specified, bacterial ... | 2016 | 27028756 |
| antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin k1 and lactobacillus rhamnosus gg. | vitamin k (vk), an essential nutrient associated with the clotting cascade, has also been demonstrated to have anticancer properties in various cancer cells including colon cancer cells. also probiotics have gained interest as potential anticancer agents. among them, lactobacillus rhamnosus gg (l.gg) has been shown to inhibit cell proliferation and polyamine biosynthesis as well as to induce apoptosis in different human gastrointestinal cancer cells. nevertheless, the exact mechanisms involved i ... | 2016 | 27035094 |
| lactobacillus rhamnosus rc007 intended for feed additive: immune-stimulatory properties and ameliorating effects on tnbs-induced colitis. | lactobacillus rhamnosus rc007 is a potential probiotic bacterium that can exert beneficial effects as supplement for animal feed, by improving the immune status in healthy host, and by providing therapeutic benefits to infected/inflamed animals. the aim of the present work was to evaluate in vivo the beneficial properties of l. rhamnosus rc007, intended for animal feed, when administered to healthy and trinitro-benzene-sulfonic-acid (tnbs) colitis induced balb/c mice. the administration of l. rh ... | 2016 | 27048833 |
| probiotics and respiratory and gastrointestinal tract infections in finnish military conscripts - a randomised placebo-controlled double-blinded study. | military conscripts are susceptible to respiratory and gastrointestinal tract infections. in previous studies probiotics have shown potency to reduce upper respiratory and gastrointestinal infections. the aim was to study whether probiotic intervention has an impact on seasonal occurrence of upper respiratory and gastrointestinal infections in two different conscript groups. in a randomised, double-blinded, placebo controlled study (https://clinicaltrials.gov nct01651195), a total of 983 healthy ... | 2016 | 27048835 |
| the probiotic lactobacillus prevents citrobacter rodentium-induced murine colitis in a tlr2-dependent manner. | the main objective of this study was to investigate whether lactobacillus rhamnosus gg (lgg) ameliorated the effects of citrobactor rodentium infection in toll-like receptor 2 (tlr2) knockout (ko) and tlr4 ko mice, as well as in wild-type c57bl/6 (b6) mice. tlr2 ko, tlr4 ko, and b6 mice were divided into three groups per each strain. each group had an uninfected control group (n = 5), c. rodentium-infected group (n = 8), and lgg-pretreated c. rodentium-infected group (n = 8). the survival rate o ... | 2016 | 27056471 |
| preserving viability of lactobacillus rhamnosus gg in vitro and in vivo by a new encapsulation system. | probiotics have shown beneficial effects on health and prevention of diseases in humans. however, a concern for application of probiotics is the loss of viability during storage and gastrointestinal transit. the aim of this study was to develop an encapsulation system to preserve viability of probiotics when they are administrated orally and apply lactobacillus rhamnosus gg (lgg) as a probiotic model to evaluate the effectiveness of this approach using in vitro and in vivo experiments. lgg was e ... | 2016 | 27063422 |
| the n-terminal gypsy motif is required for pilin-specific sortase srtc1 functionality in lactobacillus rhamnosus strain gg. | predominantly identified in pathogenic gram-positive bacteria, sortase-dependent pili are also found in commensal species, such as the probiotic-marketed strain lactobacillus rhamnosus strain gg. pili are typically associated with host colonization, immune signalling and biofilm formation. comparative analysis of the n-terminal domains of pilin-specific sortases from various piliated gram-positive bacteria identified a conserved motif, called gypsy, within the signal sequence. we investigated th ... | 2016 | 27070897 |
| faecal metaproteomic analysis reveals a personalized and stable functional microbiome and limited effects of a probiotic intervention in adults. | recent metagenomic studies have demonstrated that the overall functional potential of the intestinal microbiome is rather conserved between healthy individuals. here we assessed the biological processes undertaken in-vivo by microbes and the host in the intestinal tract by conducting a metaproteome analysis from a total of 48 faecal samples of 16 healthy adults participating in a placebo-controlled probiotic intervention trial. half of the subjects received placebo and the other half consumed la ... | 2016 | 27070903 |
| studies on anti-inflammatory and analgesic properties of lactobacillus rhamnosus in experimental animal models. | nonsteroidal anti-inflammatory drugs (nsaids) are frequently used for the treatment of inflammatory diseases. however, constant use of nsaid may lead to some side effects like gastrointestinal ulcers, bleeding and renal disorders. this study evaluates analgesic and anti-inflammatory activities of lactobacillus rhamnosus in female wistar rats. | 2016 | 27078675 |
| probiotics in critically ill children. | gut microflora contribute greatly to immune and nutritive functions and act as a physical barrier against pathogenic organisms across the gut mucosa. critical illness disrupts the balance between host and gut microflora, facilitating colonization, overgrowth, and translocation of pathogens and microbial products across intestinal mucosal barrier and causing systemic inflammatory response syndrome and sepsis. commonly used probiotics, which have been developed from organisms that form gut microbi ... | 2016 | 27081478 |
| polymorphisms, chromosomal rearrangements, and mutator phenotype development during experimental evolution of lactobacillus rhamnosus gg. | lactobacillus rhamnosus gg is a lactic acid bacterium widely marketed by the food industry. its genomic analysis led to the identification of a gene cluster encoding mucus-binding spacba pili, which is located in a genomic island enriched in insertion sequence (is) elements. in the present study, we analyzed by genome-wide resequencing the genomic integrity of l. rhamnosus gg in four distinct evolutionary experiments conducted for approximately 1,000 generations under conditions of no stress or ... | 2016 | 27084020 |
| probiotics [lgg-bb12 or rc14-gr1] versus placebo as prophylaxis for urinary tract infection in persons with spinal cord injury [prosciuttu]: a study protocol for a randomised controlled trial. | urinary tract infections [utis] are very common in people with spinal cord injury [sci]. utis are increasingly difficult and expensive to treat as the organisms that cause them become more antibiotic resistant. among the sci population, there is a high rate of multi-resistant organism [mro] colonisation. non-antibiotic prevention strategies are needed to prevent uti without increasing resistance. probiotics have been reported to be beneficial in preventing utis in post-menopausal women in severa ... | 2016 | 27084704 |
| probiotic lactobacilli inhibit early stages of candida albicans biofilm development by reducing their growth, cell adhesion, and filamentation. | we evaluated the inhibitory effects of the probiotic lactobacillus species on different phases of candida albicans biofilm development. quantification of biofilm growth and ultrastructural analyses were performed on c. albicans biofilms treated with lactobacillus rhamnosus, lactobacillus casei, and lactobacillus acidophilus planktonic cell suspensions as well as their supernatants. planktonic lactobacilli induced a significant reduction (p < 0.05) in the number of biofilm cells (25.5-61.8 %) dep ... | 2016 | 27087525 |