Publications

polyphasic approach for the characterization of rhizobial symbionts effective in fixing n(2) with common bean (phaseolus vulgaris l.).	common bean (phaseolus vulgaris l.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. the aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of arachis hypogaea, mucuna pruriens, and soils from various brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. forty-five el ...	2011	22159885
[the first experience of application of pcr techniques in real-time mode to diagnose bacteriemia during postoperational period in cardiosurgery patients].	the results comparison is made between pcr polymerase chain reaction techniques in real-time mode and cultural techniques of analysis of blood samples in patients with suspicion of bacteriemia during early postoperational period. from september to december 2009 43 blood samples from 37 adult patients aged 59+9.2 years were analyzed on suspicion of bacteriemia. the blood samples for pcr analysis (septifast technique roche diagnostics, switzerland) were taken parallel to the inoculation. among 43 ...	2011	22164420
effects of clinical breakpoint changes in clsi guidelines 2010/2011 and eucast guidelines 2011 on antibiotic susceptibility test reporting of gram-negative bacilli.	objectives: the aim of this study was to analyse the effects of clinical breakpoint changes in clsi 2010 and 2011 guidelines and eucast 2011 guidelines on antibiotic susceptibility testing (ast) reports. methods: in total, 3713 non-duplicate clinical isolates of enterobacteriaceae, pseudomonas aeruginosa, stenotrophomonas maltophilia and acinetobacter baumannii were analysed. inhibition zone diameters were determined for β-lactams, carbapenems, fluoroquinolones, aminoglycosides and trimethoprim/ ...	2011	22167240
removal of high concentrations of nh(3) by a combined photoreactor and biotrickling filter system.	average emission levels as high as 800 ppm(v) nh(3) have often been found during the anaerobic fermentation process. at these levels, nh(3) is regarded as an environmental toxic compound. high concentrations of nh(3) gas are difficult to treat in a single treatment process, suggesting that, in terms of economic cost and treatment performance, a coupled system may be a feasible technological alternative. in the coupled tio(2) photocatalytic-biological treatment system evaluated here, the optimal ...	2011	22175871
calcite biomineralization by bacterial isolates from the recently discovered pristine karstic herrenberg cave.	karstic caves represent one of the most important subterranean carbon storages on earth and provide windows into the subsurface. the recent discovery of the herrenberg cave, germany, gave us the opportunity to investigate the diversity and potential role of bacteria in carbonate mineral formation. calcite was the only mineral observed by raman spectroscopy to precipitate as stalactites from seepage water. bacterial cells were found on the surface and interior of stalactites by confocal laser sca ...	2011	22179248
the antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds.	the antibiotic-resistant bacteria are a major concern to wound care because of their ability to resist many of the antibiotics used today to treat infections. consequently, other antimicrobials, in particular ionic silver, are considered ideal topical agents for effectively helping to manage and prevent local infections. little is known about the antimicrobial efficacy of ionic silver on antibiotic-resistant bacteria at different ph values. consequently, in this study our aim was to evaluate the ...	2011	22182219
volatiles of two growth-inhibiting rhizobacteria commonly enroll atwrky18 function.	interactions with the (a)biotic environment play key roles for plant's fitness and vitality. in addition to direct surface-to-surface contact, volatile chemicals also affect other organisms. volatiles of serratia plymuthica and stenotrophomonas maltophilia significantly inhibited growth and induced h(2) o(2) production in arabidopsis in dual-culture. within one day, transcriptional changes could be observed by promoter-gus assays using a stress inducible, w-box containing 4xgst1 construct. expr ...	2011	22188129
reducing the development of antibiotic resistance in critical care units.	bacteria becoming resistant to an increasing number of antibiotic classes are a major problem at hospitals including critical care units worldwide. awareness of this problem and the need to prevent the development of antibiotic resistance are very important, especially since very few new antibiotics will become available in the near future. this article gives an overview of the mechanisms of antibacterial resistance and actual resistance data worldwide of the most prevalent gram positive (mrsa, ...	2011	22188438
Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs?	ABSTRACT: Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more likely to be a joint achievement of both aerobic and anaerobic bacterial consortia, refining our previous observations of aerobic degradation. ...	2011	22196374
multidrug-resistant gram-negative bacterial infections after liver transplantation - spectrum and risk factors.	objectives: gram-negative bacilli infections, especially multidrug-resistant gram-negative bacilli infections, are the leading cause of high mortality after liver transplantation. this study sought to investigate the type of infection, infection rate, pathogenic spectrum, antibiotic-resistance profile, risk factors, and epidemiology of multidrug-resistant gram-negative bacterial infection. methods: a retrospective cohort study was conducted and data of 217 liver transplant patients receiving cad ...	2011	22198738
biodiversity characterization of cellulolytic bacteria present on native chaco soil by comparison of ribosomal rna genes.	sequence analysis of the 16s ribosomal rna gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which actinobacteria, proteobacteria and acidobacteria accounted for more than 76% of all sequences. in both enriched samples, members of proteobacteria were the most frequently represented. the majority of ...	2011	22202170
trends in the susceptibility of clinically important resistant bacteria to tigecycline: results from the tigecycline in-vitro surveillance in taiwan (tist), 2006-2010.	the tigecycline in-vitro surveillance in taiwan (tist), a nationwide, prospective surveillance during 2006 to 2010, collected a total of 7,793 clinical isolates, including methicillin-resistant staphylococcus aureus (mrsa) (n=1,834), penicillin-resistant streptococcus pneumoniae (prsp) (n=423), vancomycin-resistant enterococci (vre) (n=219), extended-spectrum β-lactamase (esbl)-producing escherichia coli (n=1,141), esbl-producing klebsiella pneumoniae (n=1,330), acinetobacter baumannii (n=1,645) ...	2011	22203598
evaluation of microorganisms cultured from injured and repressed tissue regeneration sites in endangered giant aquatic ozark hellbender salamanders.	investigation into the causes underlying the rapid, global amphibian decline provides critical insight into the effects of changing ecosystems. hypothesized and confirmed links between amphibian declines, disease, and environmental changes are increasingly represented in published literature. however, there are few long-term amphibian studies that include data on population size, abnormality/injury rates, disease, and habitat variables to adequately assess changes through time. we cultured and i ...	2011	22205979
stenotrophomonas maltophilia: an emerging global opportunistic pathogen.	summary: stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. the increasing incidence of nosocomial and community-acquired s. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. s. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. infections of s. maltophilia c ...	2012	22232370
gut-associated bacteria throughout the life cycle of the bark beetle dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) and their cellulolytic activities.	dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) is an endemic economically important insect of the sierra madre occidental in mexico. this bark beetle has an atypical behavior within the genus because just one beetle couple colonizes and kills seedlings and young trees of 11 pine species. in this work, the bacteria associated with the dendroctonus rhizophagus gut were analyzed by culture-dependent and culture-independent methods. analysis of 16s rrna sequences amplified di ...	2012	22234511
endowashers: an overlooked risk for possible post-endoscopic infections.	background: prevention of post-endoscopy infections is an important objective to assure patient safety. endowashers, or high throughput irrigation water pumps, are a frequently used device on endoscopes. recommendations published by professional bodies and regulatory health agencies cover not only adequate reprocessing of fiber-endoscopes but also state accepted methods of regular microbial sampling. although major instruments like endoscopes are covered by these recommendations, other devices u ...	2011	22242094
isolation and characterization of lipase-producing bacteria in the intestine of the silkworm, bombyx mori, reared on different forage.	abstract the silkworm, bombyx mori l. (lepidoptera: bombycidae), an oligophagous insect that mainly feeds on mulberry leaves, is susceptible to entomopathogen infection when reared with tricuspid cudrania leaves. a total of 56 dominant bacterial strains, classified into 12 phylotypes based on bacteriological properties and analysis of 16s rrna genes, were isolated from the intestine of the fourth and fifth instar silkworm larvae. ten and seven phylotypes exist in the intestine of the silkworm ...	2011	22243438
the role of nutrients in the biodegradation of 2,4,6-trinitrotoluene in liquid and soil.	the widely used explosive 2,4,6-trinitrotoluene (tnt) has residues that are potentially explosive, toxic, and mutagenic. tnt and other explosives can be degraded by microorganisms; however, biostimulation is needed for process efficiency. to investigate the effectiveness of using biostimulation to degrade tnt, we added varying concentrations of a nutrient amendment consisting of inorganic salts, plant extracts, and molasses to soil and liquid media. for the inoculum we used a consortium of bacte ...	2012	22245864
nagz-dependent and nagz-independent mechanisms for the β-lactamases expression in stenotrophomonas maltophilia.	the β-n-acetylglucosaminidase (nagz), encoded by nagz gene, is a critical enzyme for the basal-level ampc derepression (ampc expression in absence of β-lactam challenge) in ampd and dacb mutants of pseudomonas aeruginosa. three mutants with a phenotype of basal-level l1 and l2 β-lactamase derepression in stenotrophomonas maltophilia have been reported, including kjδdi (ampd(i) mutant), kjδmrca (mrca mutant), and kjδdiδmrca (ampd(i) and mrca double mutant). in this study, nagz of s. maltophilia w ...	2012	22252801
multilocus sequence typing for clonality analysis of antimicrobial-resistant stenotrophomonas maltophilia strains.		2012	22259772
exploring the in situ accessibility of small subunit ribosomal rna of members of the domains bacteria and eukarya to oligonucleotide probes.	the principle that the small subunit ribosomal rna (ssu rrna) is generally accessible to oligonucleotide probes designed to have high thermodynamic affinity was tested with stenotrophomonas maltophilia, rhodobacter sphaeroides, bacillus subtilis, and saccharomyces cerevisiae. fluorescein-labeled probes, designed to have δg(overall)°=-14±1 and to avoid the potential of nucleobase-specific quenching, were used to target 20 randomly selected sites in each organism. a site was considered accessible ...	2012	22264799