Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| protection of chickens against infectious bronchitis virus with a multivalent dna vaccine and boosting with an inactivated vaccine. | the protective efficacy of dna plasmids encoding avian infectious bronchitis virus (ibv) s1, n, or m protein was investigated in chickens. chickens were inoculated monovalently (with plasmid pvax1-16s1, pvax1-16m, or pvax1-16n alone) or multivalently (combination of the three different plasmids, pvax1-16s1/m/n). a prime-boost immunization protocol against ibv was developed. chickens were immunized with the multivalent dna vaccine twice and then boosted with an inactivated vaccine once. antibody ... | 2013 | 23388447 |
| the required sample size in vaccination-challenge experiments with infectious bronchitis virus, a meta-analysis. | for statistical, animal welfare and financial reasons the choice of the number of chickens per group in experiments is important. this estimation, together with the number of tracheal organ cultures (tocs) that need to be examined from each chicken in order to assess protection, should be based on the difference in level of protection that one would like to be able to detect (effect size), the expected variability of the results between and within the chickens, the desired confidence level and t ... | 2013 | 23391176 |
| review of infectious bronchitis virus around the world. | infectious bronchitis virus (ibv) is a gamma coronavirus that causes a highly contagious disease in chickens. the virus can affect the upper respiratory tract and the reproductive tract, and some strains can cause a nephritis. different serotypes and genetic types of the virus have been identified worldwide and for the most part do not cross-protect. in addition, new types of the virus continue to arise due to mutations and recombination events in the viral genome, making this virus difficult to ... | 2012 | 23397833 |
| the proportion of specific viral subpopulations in attenuated arkansas delmarva poultry industry infectious bronchitis vaccines influences vaccination outcome. | we investigated the significance of differing proportions of specific subpopulations among commercial arkansas (ark) delmarva poultry industry (dpi) vaccines with regard to vaccination outcome. two arkdpi-derived vaccines that contain a higher proportion of viruses with s1 genes that become selected during replication in chickens exhibited more rapid establishment of those selected subpopulations in chickens, produced significantly higher viral loads in tears, and induced higher antibody respons ... | 2012 | 23397834 |
| genetic diversity of spike, 3a, 3b and e genes of infectious bronchitis viruses and emergence of new recombinants in korea. | the nucleotide sequences of a region including s1, s2, 3a, 3b and e genes of twenty-seven infectious bronchitis virus (ibv) isolates in korea between 1990-2011 were determined and phylogenetic and computational recombination analyses were conducted. the sizes of coding regions of some genes varied among ibv isolates due to deletion or insertion of nucleotides; the nucleotide similarities of s1, s2, 3a, 3b and e genes among the 27 isolates were 75.9%-100.0%, 85%-100.0%, 64.0%-100.0%, 60.4%-100.0% ... | 2013 | 23435235 |
| detection of infectious bronchitis virus serotypes by reverse transcription polymerase chain reaction in broiler chickens. | infectious bronchitis (ib) is a highly contagious disease of the respiratory and urogenital tract of chickens, caused by infectious bronchitis virus (ibv), a member of the family coronaviridae. the disease is common throughout the world where chickens are produced commercially. pcr on reverse transcribed rna is a potent technique for the detection of ibv. in comparison with classical detection methods, pcr-based techniques are both sensitive and fast. dozens of serotypes and genotypes of ibv hav ... | 2013 | 23450039 |
| continuous evolution of avian infectious bronchitis virus resulting in different variants co-circulating in southern china. | sixty field strains of avian infectious bronchitis virus (ibv) were isolated from chicken flocks in different regions of guangxi from 1985 to 2012. phylogenetic analysis of s1 subunit glycoprotein genes revealed that field isolates from 2009-2011 mostly belonged to the lx4 type, while those from 1985-2008 belonged to the hn08 type, and a few others belonged to the 4/91 type, the tw type and the mass type. in addition, it is noteworthy that no obvious regional differences were found among these 6 ... | 2013 | 23474985 |
| regulation of macrophage and dendritic cell function by pathogens and through immunomodulation in the avian mucosa. | macrophages (mph) and dendritic cells (dc) are members of the mononuclear phagocyte system. in chickens, markers to distinguish mph from dc are lacking, but whether mph and dc can be distinguished in humans and mice is under debate, despite the availability of numerous markers. mucosal mph and dc are strategically located to ingest foreign antigens, suggesting they can rapidly respond to invading pathogens. this review addresses our current understanding of dc and mph function, the receptors exp ... | 2013 | 23542704 |
| immune responses to infectious laryngotracheitis virus. | infectious laryngotracheitis (ilt) is an upper respiratory tract disease in chickens caused by infectious laryngotracheitis virus (iltv), an alphaherpesvirus. despite the extensive use of attenuated, and more recently recombinant, vaccines for the control of this disease, ilt continues to affect the intensive poultry industries worldwide. innate and cell-mediated, rather than humoral immune responses, have been identified as responsible for protection against disease. this review examines the cu ... | 2013 | 23567343 |
| avian metapneumoviruses expressing infectious bronchitis virus genes are stable and induce protection. | the study investigates the ability of subtype a avian metapneumovirus (ampv) to accept foreign genes and be used as a vector for delivery of infectious bronchitis virus (ibv) qx genes to chickens. initially the gfp gene was added to ampv at all gene junctions in conjunction with the development of cassetted full length dna ampv copies. after recombinant virus had been recovered by reverse genetics, gfp positions supporting gene expression while maintaining virus viability in vitro, were determin ... | 2013 | 23588091 |
| influenza c virus-associated community-acquired pneumonia in children. | to evaluate the impact of influenza c (icv) infection in children with community-acquired pneumonia (cap), all of the children consecutively seen during 4 influenza seasons with respiratory symptoms and radiographically confirmed cap were prospectively evaluated. icv was identified in the respiratory secretions of five of 391 patients (1·3%). in children with icv-associated cap, clinical data were similar to those observed in children with iav-associated cap and worse than those observed in chil ... | 2012 | 23594251 |
| burden of respiratory tract infections among paediatric in and out-patient units during 2010-11. | respiratory tract infections due to viral etiology were studied with an objective to identify and compare the pathogens between hospital indoor and outdoor units. | 2013 | 23609364 |
| negatively charged residues in the endodomain are critical for specific assembly of spike protein into murine coronavirus. | coronavirus spike (s) protein assembles into virions via its carboxy-terminus, which is composed of a transmembrane domain and an endodomain. here, the carboxy-terminal charge-rich motif in the endodomain was verified to be critical for the specificity of s assembly into mouse hepatitis virus (mhv). recombinant mhvs exhibited a range of abilities to accommodate the homologous s endodomains from the betacoronaviruses bovine coronavirus and human sars-associated coronavirus, the alphacoronavirus p ... | 2013 | 23628137 |
| the toxicological impacts of the fusarium mycotoxin, deoxynivalenol, in poultry flocks with special reference to immunotoxicity. | deoxynivalenol (don) is a common fusarium toxin in poultry feed. chickens are more resistant to the adverse impacts of deoxynivalenol (don) compared to other species. in general, the acute form of don mycotoxicosis rarely occurs in poultry flocks under normal conditions. however, if diets contain low levels of don (less than 5 mg don/kg diet), lower productivity, impaired immunity and higher susceptibility to infectious diseases can occur. the molecular mechanism of action of don has not been co ... | 2013 | 23628787 |
| the cellular interactome of the coronavirus infectious bronchitis virus nucleocapsid protein and functional implications for virus biology. | the coronavirus nucleocapsid (n) protein plays a multifunctional role in the virus life cycle, from regulation of replication and transcription and genome packaging to modulation of host cell processes. these functions are likely to be facilitated by interactions with host cell proteins. the potential interactome of the infectious bronchitis virus (ibv) n protein was mapped using stable isotope labeling with amino acids in cell culture (silac) coupled to a green fluorescent protein-nanotrap pull ... | 2013 | 23637410 |
| upregulation of chop/gadd153 during coronavirus infectious bronchitis virus infection modulates apoptosis by restricting activation of the extracellular signal-regulated kinase pathway. | induction of the unfolded protein response (upr) is an adaptive cellular response to endoplasmic reticulum (er) stress that allows a cell to reestablish er homeostasis. however, under severe and persistent er stress, prolonged upr may activate unique pathways that lead to cell death. in this study, we investigated the activation of the protein kinase r-like er kinase (perk) pathway of upr in cells infected with the coronavirus infectious bronchitis virus (ibv) and its relationship with ibv-induc ... | 2013 | 23678184 |
| characterization of nephropathogenic infectious bronchitis virus dmv/1639/11 recovered from delmarva broiler chickens in 2011. | a limited outbreak of nephropathogenic infectious bronchitis (nib) occurred in three delmarva (dmv) commercial broiler chicken flocks in 2011. isolates of nib virus (nibv)--dmv/1639/11, dmv/3432/11, and dmv/3902/11--were characterized by sequence analysis of the n-terminal subunit (s1) of the spike (s) gene. findings indicated that the isolates were identical to each other and to pa/9579a/10, a 2010 isolate from poultry in pennsylvania. the 2010 and 2011 isolates appear to have originated from a ... | 2013 | 23678731 |
| suppression of coronavirus replication by cyclophilin inhibitors. | coronaviruses infect a variety of mammalian and avian species and cause serious diseases in humans, cats, mice, and birds in the form of severe acute respiratory syndrome (sars), feline infectious peritonitis (fip), mouse hepatitis, and avian infectious bronchitis, respectively. no effective vaccine or treatment has been developed for sars-coronavirus or fip virus, both of which cause lethal diseases. it has been reported that a cyclophilin inhibitor, cyclosporin a (csa), could inhibit the repli ... | 2013 | 23698397 |
| experimental study on histopathological changes and tissue tropism of iranian infectious bronchitis serotype 793/b-like virus in spf chickens. | avian infectious bronchitis virus (ibv) is prevalent in all countries with intensive poultry flocks. this disease is characterised primarily by respiratory signs, but some ibv strains may also infect other organs such as the intestinal and urogenital tracts. the aim of this study was to characterise the histopathological lesions and tissue tropism of iranian isolate ir/773/2001(793/b) of avian infectious bronchitis virus in different organs of experimentally infected spf chickens. forty-two one- ... | 2013 | 23718717 |
| presence of infectious bronchitis virus strain ck/ch/ldl/97i in the middle east. | infectious bronchitis virus (ibv) is a very dynamic and evolving virus, causing major economic losses to the global poultry industry. in early 2011, respiratory disease outbreaks were investigated in iraq, jordan, and saudi arabia. five ibv isolates (joa2, joa4, saudi-1, saudi-2, and iraqi ibv) were detected by diagnostic-nested nucleocapsid rt-pcr. strain identification was characterised by sequencing and phylogenetic analysis of the amplified hypervariable region of the spike 1 (s1) gene. thes ... | 2012 | 23738118 |
| serosurvey for selected infectious agents in two sympatric species of cormorants (phalacrocorax atriceps and phalacrocorax magellanicus) from coastal patagonia, argentina. | we conducted a serologic survey for selected infectious agents on two sympatric cormorants, the imperial cormorant (phalacrocorax atriceps) and the rock shag (phalacrocorax magellanicus). blood was collected from 267 imperial cormorants and 106 rock shags at 17 colonies along the patagonia atlantic shore during nine breeding seasons (1994, 1999-2001-2005-2008-2010). antibodies to four pathogens were common to both species and frequently observed: avian paramyxovirus type 1 (56% of imperial cormo ... | 2013 | 23778597 |
| a cholesterol tag at the n terminus of the relatively broad-spectrum fusion inhibitory peptide targets an earlier stage of fusion glycoprotein activation and increases the peptide's antiviral potency in vivo. | in previous work, we designed peptides that showed potent inhibition of newcastle disease virus (ndv) and infectious bronchitis virus (ibv) infections in chicken embryos. in this study, we demonstrate that peptides modified with cholesterol or 3 u of polyethylene glycol (peg3) conjugated to the peptides' n termini showed even more promising antiviral activities when tested in animal models. both cholesterol- and cholesterol-peg3-tagged peptides were able to protect chicken embryos from infection ... | 2013 | 23804636 |
| a duplex sybr green i-based real-time rt-pcr assay for the simultaneous detection and differentiation of massachusetts and non-massachusetts serotypes of infectious bronchitis virus. | infectious bronchitis is a highly contagious viral disease of poultry caused by infectious bronchitis virus (ibv) and is considered one of the most economically important viral diseases of chickens. control of ibv has been attempted using live attenuated and inactivated vaccines. live attenuated vaccines of the massachusetts (mass.) serotype are the most commonly used for this purpose. due to the continuous emergence of new variants of the infectious bronchitis virus, the identification of the t ... | 2013 | 23810983 |
| lactococcus lactis anchoring avian infectious bronchitis virus multi-epitope peptide epic induced specific immune responses in chickens. | mucosal immunity is critical in preventing infectious bronchitis virus (ibv) infection. to deliver viral antigens to the mucosal immune system of chickens safely and effectively, we constructed a lactococcus lactis strain carrying ibv multi-epitope gene epic fused with the gene of the cell-wall anchoring domain of staphylococcus aureus protein a. sds-page and western blot results indicated that the fused peptide was located partially on the cell surface. oral and nasal inoculation with the recom ... | 2013 | 23832344 |
| infectious bronchitis virus as a vector for the expression of heterologous genes. | the avian coronavirus infectious bronchitis virus (ibv) is the causative agent of the respiratory disease infectious bronchitis of domestic fowl, and is controlled by routine vaccination. to explore the potential use of ibv as a vaccine vector a reverse genetics system was utilised to generate infectious recombinant ibvs (ribvs) expressing the reporter genes enhanced green fluorescent protein (egfp) or humanised renilla luciferase (hrluc). infectious ribvs were obtained following the replacement ... | 2013 | 23840781 |
| simultaneous detection of five major serotypes of avian coronavirus by a multiplex microsphere-based assay. | avian coronavirus (commonly known as infectious bronchitis virus [ibv]) is of major economic importance to commercial chicken producers worldwide. due to the existence of multiple serotypes and variants of the virus that do not cross-protect, it is important to diagnose circulating serotypes and choose the right vaccine type for successful protection. in an effort to improve conventional diagnostic tests, a microsphere-based assay was developed and evaluated for simultaneous detection of the mos ... | 2013 | 23847226 |
| identification of two atr-dependent phosphorylation sites on coronavirus nucleocapsid protein with nonessential functions in viral replication and infectivity in cultured cells. | coronavirus encodes an extensively phosphorylated and highly basic nucleocapsid (n) protein. previous studies have identified ser190, ser192, thr378 and ser379 as the phosphorylation sites for coronavirus infectious bronchitis virus (ibv) n protein. in this study, we show that phosphorylation at thr378 and ser379 sites is dependent on the ataxia-telangiectasia mutated (atm) and rad3-related (atr), a kinase activated during ibv replication. introduction of ala substitutions at these two sites ind ... | 2013 | 23849791 |
| humoral and cell-mediated immune responses to different doses of attenuated vaccine against avian infectious bronchitis virus. | the antibody and cellular immune responses against infectious bronchitis virus (ibv) were evaluated at mucosal sites of chickens after immunization with various doses of an attenuated vaccine at 1 day of age. the correlation of these immune responses with protection of tracheal tissues was evaluated after experimental infection of these birds. significantly reduced tracheal pathologic effects, measured according to ciliostasis and histology lesions, and reduced viral load were observed only in t ... | 2013 | 23865943 |
| changes of several acute phase factors in broiler chickens in response to infectious bronchitis virus infection. | this study was performed to clarify the acute phase response following infectious bronchitis virus inoculation. ninety clinically healthy 1-d-old ross chicks were randomly assigned into 2 groups: control (n = 20) and infected group (n = 70). at the age of 20 d, all birds in the infected group were challenged intranasally with allantoic fluid containing 10(5) embryo lethal dose (eld50)/0.1 ml of the infectious bronchitis virus. blood samples were collected from 20 clinically healthy and 70 infect ... | 2013 | 23873545 |
| sialic acid binding properties of soluble coronavirus spike (s1) proteins: differences between infectious bronchitis virus and transmissible gastroenteritis virus. | the spike proteins of a number of coronaviruses are able to bind to sialic acids present on the cell surface. the importance of this sialic acid binding ability during infection is, however, quite different. we compared the spike protein of transmissible gastroenteritis virus (tgev) and the spike protein of infectious bronchitis virus (ibv). whereas sialic acid is the only receptor determinant known so far for ibv, tgev requires interaction with its receptor aminopeptidase n to initiate infectio ... | 2013 | 23896748 |
| application of a new bivalent marek's disease vaccine does not interfere with infectious bronchitis or newcastle disease vaccinations and proves efficacious. | a possible interference after marek's disease (md) vaccination using an experimental bivalent vaccine, consisting of a redesigned cvi-988/rispens-type mdv-1 strain and herpesvirus of turkeys, with vaccination against infectious bronchitis (ib) virus (ibv) or newcastle disease (nd) virus (ndv) was examined. day-old specific-pathogen-free chicks were concomitantly vaccinated with the bivalent md vaccine (either intramuscularly or subcutaneously) and with commercially available vaccines against nd ... | 2013 | 23901767 |
| [development of a gexp assay for simultaneous differentiation of six chicken respiratory viruses]. | a gexp based multiplex pcr assay was developed to simultaneously detect six different chicken respiratory viruses including h5, h7, h9 subtypes of avian influenza virus(aiv), new castle disease virus (ndv), infectious bronchitis virus(ibv) and infectious laryngotracheitis virus(iltv). according to the conserved sequences of genes of each pathogen, seven pairs of specific primers were designed, and the reaction conditions were optimized. the specificity and accuracy of gexp were examined using sa ... | 2013 | 23905467 |
| cell-mediated immune responses in the head-associated lymphoid tissues induced to a live attenuated avian coronavirus vaccine. | humoral immunity is important for controlling viral diseases of poultry, but recent studies have indicated that cytotoxic t cells also play an important role in the immune response to infectious bronchitis virus (ibv). to better understand the cell mediated immune responses to ibv in the mucosal and systemic immune compartments chickens were ocularly vaccinated with ibv. this induced a lymphocyte expansion in head-associated lymphoid tissues (halt) and to a lesser extent in the spleen, followed ... | 2013 | 23948147 |
| altered pro-inflammatory cytokine mrna levels in chickens infected with infectious bronchitis virus. | infectious bronchitis virus (ibv) replicates primarily in the respiratory tract and grows in various organs in chickens, with or without pathological effects. the diversity of this virus has been verified by sequence analysis of the s1 glycoprotein gene, but this method must be supplemented with further analysis for characterization of the agent. to increase our understanding of the pathogenesis of the disease caused by this virus, we investigated the response of chickens to 2 ibv with different ... | 2013 | 23960111 |
| decay of maternal antibodies in broiler chickens. | the objective of this study was to determine the decay rate of maternal antibodies against major broiler chicken pathogens. a total of 30 one-day-old broiler chicks were obtained from a commercial hatchery and reared in isolation. these chicks were retrieved from a parent flock that received a routine vaccination program. chicks were bled at hatch and sequentially thereafter every 5 d through 30 d of age. maternal antibody titers were measured by elisa for avian encephalomyelitis (aev), avian in ... | 2013 | 23960115 |
| role of chicken astrovirus as a causative agent of gout in commercial broilers in india. | several outbreaks of gout were reported in commercial broilers in india during 2011 and 2012, causing up to 40% mortality in the birds. gross and histopathological observations confirmed gout. quantitative reverse transcriptase polymerase chain reaction (qrt-pcr) analysis from kidney samples of gout-affected birds indicated the presence of chicken astrovirus (castv) in 41.7% of cases and a mixed infection of castv and avian nephritis virus (anv) in 36.4% of cases. castv isolated from gout-affect ... | 2013 | 24015918 |
| identification and survey of a novel avian coronavirus in ducks. | the rapid discovery of novel viruses using next generation sequencing (ngs) technologies including dna-seq and rna-seq, has greatly expanded our understanding of viral diversity in recent years. the timely identification of novel viruses using ngs technologies is also important for us to control emerging infectious diseases caused by novel viruses. in this study, we identified a novel duck coronavirus (cov), distinct with chicken infectious bronchitis virus (ibv), using rna-seq. the novel duck-s ... | 2013 | 24023656 |
| contributions of the s2 spike ectodomain to attachment and host range of infectious bronchitis virus. | the spike protein is the major viral attachment protein of the avian coronavirus infectious bronchitis virus (ibv) and ultimately determines viral tropism. the s1 subunit of the spike is assumed to be required for virus attachment. however, we have previously shown that this domain of the embryo- and cell culture adapted beaudette strain, in contrast to that of the virulent m41 strain, is not sufficient for binding to chicken trachea (wickramasinghe et al., 2011). in the present study, we demons ... | 2013 | 24041648 |
| assembly and immunogenicity of coronavirus-like particles carrying infectious bronchitis virus m and s proteins. | infectious bronchitis virus (ibv) as an avian coronavirus is still posing a persistent and imminent threat to the poultry industry worldwide. here we report that transfection of sf9 cells with a single recombinant baculovirus encoding m and s proteins resulted in the assembly of ibv vlps; this is the first report that s protein plus m protein alone were able to be assembled into vlps for coronaviruses. we further showed that the generated ibv vlps could induce humoral immune responses in a level ... | 2013 | 24099870 |
| complete genome sequence of a nephropathogenic infectious bronchitis virus strain isolated in china. | infectious bronchitis virus (ibv) causes tremendous economic losses to the poultry industry. here, we report the complete genome analysis results for a new natural recombination nephropathogenic ibv strain named saibk, which was isolated in the sichuan province of china in 2005. | 2013 | 24115543 |
| molecular survey of enteric viruses in commercial chicken farms in korea with a history of enteritis. | several enteric viruses have increasingly received attention as potential causative agents of runting-stunting syndrome (rss) in chickens. a molecular survey was performed to determine the presence of a broad range of enteric viruses, namely chicken astrovirus (castv), avian nephritis virus (anv), chicken parvovirus (chpv), infectious bronchitis virus (ibv), avian rotavirus (avrv), avian reovirus (arv), and fowl adenovirus (fadv), in intestinal samples derived from 34 commercial chicken flocks t ... | 2013 | 24135590 |
| infectious bronchitis virus generates spherules from zippered endoplasmic reticulum membranes. | replication of positive-sense rna viruses is associated with the rearrangement of cellular membranes. previous work on the infection of tissue culture cell lines with the betacoronaviruses mouse hepatitis virus and severe acute respiratory syndrome coronavirus (sars-cov) showed that they generate double-membrane vesicles (dmvs) and convoluted membranes as part of a reticular membrane network. here we describe a detailed study of the membrane rearrangements induced by the avian gammacoronavirus i ... | 2013 | 24149513 |
| transcriptome analysis of chicken kidney tissues following coronavirus avian infectious bronchitis virus infection. | infectious bronchitis virus (ibv), a prototype of the coronaviridae family, is an economically important causative agent of infectious bronchitis in chickens and causes an acute and highly contagious upper respiratory tract infections that may lead to nephritis. however, the molecular antiviral mechanisms of chickens to ibv infection remain poorly understood. in this study, we conducted global gene expression profiling of chicken kidney tissue after nephropathogenic ibv infection to better under ... | 2013 | 24168272 |
| discovery of a novel bottlenose dolphin coronavirus reveals a distinct species of marine mammal coronavirus in gammacoronavirus. | while gammacoronaviruses mainly comprise infectious bronchitis virus (ibv) and its closely related bird coronaviruses (covs), the only mammalian gammacoronavirus was discovered from a white beluga whale (beluga whale cov [bwcov] sw1) in 2008. in this study, we discovered a novel gammacoronavirus from fecal samples from three indo-pacific bottlenose dolphins (tursiops aduncus), which we named bottlenose dolphin cov (bdcov) hku22. all the three bdcov hku22-positive samples were collected on the sa ... | 2014 | 24227844 |
| cross-protective immune responses elicited by a korean variant of infectious bronchitis virus. | infectious bronchitis virus (ibv) infections cause great economic losses to the poultry industry worldwide. ibvs continuously evolve by developing mutations in antigenic sites; therefore, an ibv vaccine that provides broad cross-protection can be a highly relevant and practical method in ibv control strategies. although some ibv vaccine strains are known to provide protection against multiple ibv serotypes, in general commercially available ibv vaccine strains provide protection against antigeni ... | 2013 | 24283135 |
| development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of s1 gene of h120 strain. | infectious bronchitis (ib), caused by infectious bronchitis virus (ibv), is a highly contagious chicken disease, and can lead to serious economic losses in poultry enterprises. the continual introduction of new ibv serotypes requires alternative strategies for the production of timely and safe vaccines against the emergence of variants. modification of the ibv genome using reverse genetics is one way to generate recombinant ibvs as the candidates of new ibv vaccines. in this study, the recombina ... | 2014 | 24287931 |
| molecular characterization of major structural protein genes of avian coronavirus infectious bronchitis virus isolates in southern china. | to gain comprehensive genetic information of circulating avian coronavirus infectious bronchitis virus (ibv) isolates in china, analysis of the phylogenetic tree, entropy of the amino acid sequences, and the positive selection as well as computational recombinations of s1, m and n genes of 23 ibv isolates was conducted in the present study. the phylogenetic trees based on the s1, m and n genes exhibited considerably different topology and the ck/ch/lsc/99i-type isolates were the predominant ibvs ... | 2013 | 24304696 |
| adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations. | mannose-binding lectin (mbl) plays a major role in the immune response as a soluble pattern-recognition receptor. mbl deficiency and susceptibility to different types of infections have been subject to extensive studies over the last decades. in humans and chickens, several studies have shown that mbl participates in the protection of hosts against virus infections. infectious bronchitis (ib) is a highly contagious disease of economic importance in the poultry industry caused by the coronavirus ... | 2014 | 24305086 |
| neuraminidase subtyping of avian influenza viruses with primerhunter-designed primers and quadruplicate primer pools. | we have previously developed a software package called primerhunter to design primers for pcr-based virus subtyping. in this study, 9 pairs of primers were designed with primerhunter and successfully used to differentiate the 9 neuraminidase (na) genes of avian influenza viruses (aivs) in multiple pcr-based assays. furthermore, primer pools were designed and successfully used to decrease the number of reactions needed for na subtyping from 9 to 4. the quadruplicate primer-pool method is cost-sav ... | 2013 | 24312367 |
| mapping of the receptor-binding domain and amino acids critical for attachment in the spike protein of avian coronavirus infectious bronchitis virus. | the infection of the avian coronavirus infectious bronchitis virus (ibv) is initiated by the binding of the spike glycoprotein s to sialic acids on the chicken host cell. in this study we identified the receptor-binding domain (rbd) of the spike of the prototype ibv strain m41. by analyzing the ability of recombinantly expressed chimeric and truncated spike proteins to bind to chicken tissues, we demonstrate that the n-terminal 253 amino acids of the spike are both required and sufficient for bi ... | 2014 | 24314633 |
| a genome-wide association study identifies major loci affecting the immune response against infectious bronchitis virus in chicken. | coronaviruses are a hot research topic because they can cause severe diseases in humans and animals. infectious bronchitis virus (ibv), belonging to gamma-coronaviruses, causes a highly infectious respiratory viral disease and can result in catastrophic economic losses to the poultry industry worldwide. unfortunately, the genetic basis of the host immune responses against ibv is poorly understood. in the present study, the antibody levels against ibv post-immunization were measured by an enzyme- ... | 2014 | 24333371 |
| bacmam virus-based surface display of the infectious bronchitis virus (ibv) s1 glycoprotein confers strong protection against virulent ibv challenge in chickens. | avian infectious bronchitis virus (ibv) is associated with production inefficiencies in domestic fowl, and causes massive economic losses to the poultry industry worldwide. progress has been made in designing novel and efficient candidate vaccines to control ibv infection. bacmam virus, a modified baculovirus mediating transgene expression under the control of a mammalian promoter, has emerged as a versatile and safe vector during vaccine development. in previous work, we generated the bacmam vi ... | 2014 | 24342247 |
| how the double spherules of infectious bronchitis virus impact our understanding of rna virus replicative organelles. | powered by advances in electron tomography, recent studies have extended our understanding of how viruses construct "replication factories" inside infected cells. their function, however, remains an area of speculation with important implications for human health. it is clear from these studies that whatever their purpose, organelle structure is dynamic (m. ulasli, m. h. verheije, c. a. de haan, and f. reggiori, cell. microbiol. 12:844-861, 2010) and intricate (k. knoops, m. kikkert, s. h. worm, ... | 2013 | 24345746 |
| diagnostic and clinical observation on the infectious bronchitis virus strain q1 in italy. | this paper describes the diagnostic and clinical observations of an infectious bronchitis virus (ibv) variant, referred to as q1, in clinically ill chickens in italy. this ibv variant was described for the first time in 1998 in china. in the autumn of 2011 it caused a small-scale epidemic in nonvaccinated meat chickens in farms located in northern italy. the disease was characterized by increased mortality, kidney lesions and proventriculitis. histopathological observations confirmed the nephrit ... | 2013 | 24362775 |
| production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus. | infectious bronchitis virus (ibv) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. vaccination is the most effective method of preventing infection and controlling the spread of ibv, but currently available inactivated and attenuated virus vaccines have some disadvantages. we developed a chimeric virus-like particle (vlp)-based candidate vaccine for ibv protection. the chimeric vlp was composed of matrix 1 protein from avian influenza h5n1 virus and a fus ... | 2013 | 24378590 |
| emerging viral diseases of livestock in the developing world. | emerging and reemerging viral diseases of livestock and human beings are in sharp rise in recent years. importantly, many of these viruses, including influenza, hendra, nipah and corona are of zoonotic importance. several viral diseases of livestock such as bluetongue, peste des petits ruminants, camel pox, equine infectious anaemia, chicken anaemia and sheep-associated malignant catarrhal fever are crossing their traditional boundaries. emergence of new serotypes and variant forms of viruses as ... | 2013 | 24426290 |
| sambucus nigra extracts inhibit infectious bronchitis virus at an early point during replication. | infectious bronchitis virus (ibv) is a pathogenic chicken coronavirus. currently, vaccination against ibv is only partially protective; therefore, better preventions and treatments are needed. plants produce antimicrobial secondary compounds, which may be a source for novel anti-viral drugs. non-cytotoxic, crude ethanol extracts of rhodiola rosea roots, nigella sativa seeds, and sambucus nigra fruit were tested for anti-ibv activity, since these safe, widely used plant tissues contain polyphenol ... | 2014 | 24433341 |
| modeling inoculum dose dependent patterns of acute virus infections. | inoculum dose, i.e. the number of pathogens at the beginning of an infection, often affects key aspects of pathogen and immune response dynamics. these in turn determine clinically relevant outcomes, such as morbidity and mortality. despite the general recognition that inoculum dose is an important component of infection outcomes, we currently do not understand its impact in much detail. this study is intended to start filling this knowledge gap by analyzing inoculum dependent patterns of viral ... | 2014 | 24440713 |
| universal influenza b virus genomic amplification facilitates sequencing, diagnostics, and reverse genetics. | although human influenza b virus (ibv) is a significant human pathogen, its great genetic diversity has limited our ability to universally amplify the entire genome for subsequent sequencing or vaccine production. the generation of sequence data via next-generation approaches and the rapid cloning of viral genes are critical for basic research, diagnostics, antiviral drugs, and vaccines to combat ibv. to overcome the difficulty of amplifying the diverse and ever-changing ibv genome, we developed ... | 2014 | 24501036 |
| induction of innate immune response following infectious bronchitis corona virus infection in the respiratory tract of chickens. | infectious bronchitis virus (ibv) replicates in the epithelial cells of trachea and lungs of chicken, however the mechanism of generation of innate immune response against ibv infection in these tissues has not been fully characterized. our objective was to study innate responses induced early following ibv infection in chickens. initiation of the transcription of selected innate immune genes such as tlr3, tlr7, myd88, il-1β and ifn-β, as well as recruitment of macrophages, were evident followin ... | 2014 | 24503073 |
| emergence of enteric viruses in production chickens is a concern for avian health. | several viruses have been identified in recent years in the intestinal contents of chickens and turkeys with enteric problems, which have been observed in commercial farms worldwide, including brazil. molecular detection of these viruses in brazil can transform to a big threat for poultry production due to risk for intestinal integrity. this disease is characterized by severely delayed growth, low uniformity, lethargy, watery diarrhea, delayed feed consumption, and a decreased conversion rate. c ... | 2014 | 24578633 |
| high-level protein expression following single and dual gene cloning of infectious bronchitis virus n and s genes using baculovirus systems. | baculovirus is an efficient system for the gene expression that can be used for gene transfer to both insect and different vertebrate hosts. the nucleocapsid gene (n) of the infectious bronchitis virus was cloned in a baculovirus expression system for insect cell expression. dual expression vectors containing ibv n and spike (s) proteins of the avian infectious bronchitis virus were engineered under the control of human and murine cytomegalovirus immediate-early enhancer/promoter elements in com ... | 2014 | 24605790 |
| comparative proteome analysis of tracheal tissues in response to infectious bronchitis coronavirus, newcastle disease virus, and avian influenza virus h9 subtype virus infection. | infectious bronchitis coronavirus (ibv), newcastle disease virus (ndv), and avian influenza virus (aiv) h9 subtype are major pathogens of chickens causing serious respiratory tract disease and heavy economic losses. to better understand the replication features of these viruses in their target organs and molecular pathogenesis of these different viruses, comparative proteomic analysis was performed to investigate the proteome changes of primary target organ during ibv, ndv, and aiv h9 infections ... | 2014 | 24610701 |
| effects of avian infectious bronchitis virus antigen on eggshell formation and immunoreaction in hen oviduct. | the aim of this study was to determine the mechanism by which the avian infectious bronchitis virus (ibv) affects eggshell formation. attenuated ibv (aibv group) or vehicle (control group) was injected into the oviductal magnum lumen of white leghorn laying hens. the changes in the expression of genes related to eggshell formation (collagen types i and v, and cabp-d28k), densities of cytotoxic cells (cd8(+) and tcr-γδ(+) t cells), and gene expression of molecules related to cytotoxic immunoreact ... | 2014 | 24612789 |
| origin and characteristics of the recombinant novel avian infectious bronchitis coronavirus isolate ck/ch/ljl/111054. | recombination among infectious bronchitis viruses (ibvs), coupled with point mutations, insertions, and deletions that occur in the genome, is thought to contribute to the emergence of new ibv variants. in this study an ibv, ck/ch/ljl/111054, was isolated from a h120-vaccinated chicken, which presented with a suspected ibv infection. phylogenetic analysis of the s1 subunit sequence confirmed that strain ck/ch/ljl/111054 is of the connecticut-type; however, further extensive full-length genomic a ... | 2014 | 24613433 |
| palmitoylation is required for intracellular trafficking of influenza b virus nb protein and efficient influenza b virus growth in vitro. | all influenza viruses bud and egress from lipid rafts within the apical plasma membrane of infected epithelial cells. as a result, all components of progeny virions must be transported to these lipid rafts for assembly and budding. although the mechanism of transport for other influenza proteins has been elucidated, influenza b virus (ibv) glycoprotein nb subcellular localization and transport are not understood completely. to address the aforementioned properties of nb, a series of trafficking ... | 2014 | 24671751 |
| in this issue - april 2014: perceptions of 2007 equine influenza outbreak · hyperinsulinaemia in ponies · hydration and perennial ryegrass toxicosis · travel history needed for possible canine leishmaniosis · complications of arthroscopy · metaphyseal osteopathy in kelpies · infectious bronchitis virus and egg quality. | 2014 | 24673131 | |
| assessment of the potential relationship between egg quality and infectious bronchitis virus infection in australian layer flocks. | this investigation aimed to determine if there was a relationship between the production of eggs with poor internal quality, as measured by poor haugh units, by australian layer flocks and the detection of infectious bronchitis virus (ibv) in the hens. other risk factors including flock size, flock type, flock age, chicken breed and vaccination frequency were also assessed. | 2014 | 24673141 |
| pathogenicity and molecular characteristics of infectious bronchitis virus (ibv) strains isolated from broilers showing diarrhoea and respiratory disease. | abstract 1. the possibility that infectious bronchitis virus (ibv) variants isolated from broilers with enteric and respiratory problems have a different tropism and pathological outcome from those ibv strains causing classical respiratory disease was investigated. 2. ibv variants were isolated from broiler flocks with enteric and respiratory problems in two regions of brazil. the usp-10 isolate, of enteric origin, was inoculated via the oral oroculonasal routes into ibv-antibody-free broilers a ... | 2014 | 24678626 |
| complete genome sequence of a novel infectious bronchitis virus strain circulating in china with a distinct s gene. | an avian infectious bronchitis virus (ibv) was isolated and identified from a commercial layer flock vaccinated with live attenuated h120 vaccine in china, designed as ck/ch/ibtz/2012. to determine the origination and evolution of this isolated strain, we have carried out a complete genome sequencing of this strain. the genome of the ck/ch/ibtz/2012 strain is 27,691 nucleotides in length and includes more than 10 open reading frames. sequence comparison and phylogenetic analysis based on the ful ... | 2014 | 24682939 |
| emergence of a new genotype of avian infectious bronchitis virus in brazil. | infectious bronchitis virus (ibv) is the agent of a highly contagious disease that affects domestic fowl (gallus gallus). recent reports showed a high prevalence of one main ibv genotype (brazil or br-i) with low genetic diversity in commercial poultry flocks from brazil. this research analyzed ibv positive poultry flocks from different rearing regions to verify the s1 gene variability and geographic distribution of variant ibv strains in recent years (2010 and 2011). samples of ibv-positive flo ... | 2013 | 24689178 |
| evaluation of infectious bronchitis virus arkansas-type vaccine failure in commercial broilers. | infectious bronchitis virus (ibv) causes an upper respiratory tract disease in chickens and is highly contagious. many different types of the virus exist, but only a few types are used as attenuated live vaccines in the commercial poultry industry. of the vaccine types used, the arkansas (ark)-type virus is most frequently reisolated from vaccinated broilers. previous research has suggested that incomplete clearance of ark-type vaccine virus plays a role in the inadequate protection observed whe ... | 2013 | 24689182 |
| dramatic differences in the response of macrophages from b2 and b19 mhc-defined haplotypes to interferon gamma and polyinosinic:polycytidylic acid stimulation. | the chicken mhc has been associated with disease resistance, though the mechanisms are not understood. the functions of macrophages, critical to both innate and acquired immunity, were compared between the more infectious bronchitis virus-resistant b2 and the more infectious bronchitis virus-susceptible b19 lines. in vivo peripheral blood concentrations of monocytes were similar in b2 or b19 homozygous haplotypes. peripheral blood-derived macrophages were stimulated with poly i:c, simulating an ... | 2014 | 24706959 |
| evaluation of a novel strain of infectious bronchitis virus emerged as a result of spike gene recombination between two highly diverged parent strains. | the emergence of new variant strains of the poultry pathogen infectious bronchitis virus (ibv) is continually reported worldwide, owing to the labile nature of the large single-stranded rna ibv genome. high resolution melt curve analysis previously detected a variant strain, n1/08, and the present study confirmed that this strain had emerged as a result of recombination between australian subgroup 2 and 3 strains in the spike gene region, in a similar manner reported for turkey coronaviruses. th ... | 2014 | 24730363 |
| genotyping of infectious bronchitis viruses identified in canada between 2000 and 2013. | infectious bronchitis virus (ibv) was detected in 185 samples originating from chicken flocks of various commodity groups in canada. flocks with clinical signs such as respiratory challenge, sudden death, egg production problems, or nephropathogenic conditions, and randomly selected flocks sampled at slaughter as part of an ontario broiler surveillance project, were included. most samples were from ontario and québec; however, a small number from british columbia, nova scotia, and newfoundland a ... | 2014 | 24754612 |
| infectious bronchitis virus s2 expressed from recombinant virus confers broad protection against challenge. | we developed a recombinant newcastle disease virus (ndv) lasota (rls) expressing the infectious bronchitis virus (ibv) s2 gene (rls/ibv.s2). the recombinant virus showed somewhat-reduced pathogenicity compared to the parental lentogenic lasota strain but effectively elicited hemagglutination inhibition antibodies against ndv and protected chickens against lethal challenge with virulent ndv/ca02. ibv heterotypic protection was assessed using a prime-boost approach with a commercially available at ... | 2014 | 24758118 |
| comparison of vaccine subpopulation selection, viral loads, vaccine virus persistence in trachea and cloaca, and mucosal antibody responses after vaccination with two different arkansas delmarva poultry industry -derived infectious bronchitis virus vaccines. | factors responsible for the persistence of arkansas delmarva poultry industry (arkdpi)-derived infectious bronchitis vaccines in commercial flocks and the high frequency of isolation of arkdpi-type infectious bronchitis viruses in respiratory cases are still unclear. we compared dynamics of vaccine viral subpopulations, viral loads, persistence in trachea and cloaca, and the magnitude of infectious bronchitis virus (1bv)-specific antibody induction after vaccination with two commercial arkdpi-de ... | 2014 | 24758121 |
| emergence of avian infectious bronchitis in a non-vaccinating country. | infectious bronchitis virus (ibv) is a coronavirus of the chicken. it is a highly contagious pathogen and in addition to causing respiratory and kidney diseases can affect the reproductive organs, resulting in loss of production and poor egg quality. despite the global distribution of ibv, finland has been free of clinical cases for almost three decades. since april 2011, outbreaks involving genotypes qx, d274-like and 4/91-like have occurred in southern finland. the clinical samples studied wer ... | 2014 | 24766156 |
| infectious bronchitis virus in different avian physiological systems-a field study in brazilian poultry flocks. | avian infectious bronchitis is a highly contagious viral disease with economic effects on poultry agribusiness. the disease presents multi-systemic clinical signs (respiratory, renal, enteric, and reproductive) and is caused by one coronavirus (infectious bronchitis virus, ibv). infectious bronchitis virus is classified into different serotypes and genotypes (vaccine strains and field variants). this study aimed to evaluate the occurrence of ibv in commercial poultry flocks from 3 important prod ... | 2014 | 24894532 |
| [genotypes and serotypes of avian infectious bronchitis viruses isolated during 2009-2011 in guangxi, china]. | in order to investigate the prevalence and track genetic and antigenic evolutions of infectious bronchitis virus (ibv) and their prevalence in guangxi, china since 1985, gene amplification and sequencing and virus neutralization (vn) test on chicken embryo tracheal organ cultures were used in genotyping and serotyping of 28 ibv isolates during 2009-2011 in guangxi. the results of n gene sequencing and comparison showed that the 28 isolates and reference strains were classified into three groups, ... | 2014 | 24923170 |
| differences in the tissue tropism to chicken oviduct epithelial cells between avian coronavirus ibv strains qx and b1648 are not related to the sialic acid binding properties of their spike proteins. | the avian coronavirus (avcov) infectious bronchitis virus (ibv) is a major poultry pathogen. a characteristic feature of ibv is the occurrence of many different strains belonging to different serotypes, which makes a complete control of the disease by vaccinations a challenging task. reasons for differences in the tissue tropism and pathogenicity between ibv strains, e.g. a predilection for the kidneys or the oviduct are still an open question. strains of the qx genotype have been major pathogen ... | 2014 | 24928425 |
| effect of different levels of dietary sweet orange (citrus sinensis) peel extract on humoral immune system responses in broiler chickens. | this experiment was conducted to evaluate the effects of different levels of sweet orange (citrus sinensis) peel extract (sope) on humoral immune system responses in broiler chickens. three hundred 1-day broilers (ross-308) were randomly allocated to treatments varying in supplemental sope added in the drinking water. the experimental groups consisted of three treatments fed for 42 days as follows: a control treatment without feed extract, a treatment containing 1000 ppm of sope and a treatment ... | 2015 | 24990585 |
| coronavirus nsp6 restricts autophagosome expansion. | autophagy is a cellular response to starvation that generates autophagosomes to carry long-lived proteins and cellular organelles to lysosomes for degradation. activation of autophagy by viruses can provide an innate defense against infection, and for (+) strand rna viruses autophagosomes can facilitate assembly of replicase proteins. we demonstrated that nonstructural protein (nsp) 6 of the avian coronavirus, infectious bronchitis virus (ibv), generates autophagosomes from the er. a statistical ... | 2014 | 24991833 |
| analysis of s1 gene of avian infectious bronchitis virus isolated in southern china during 2011-2012. | sixty-two strains of avian infectious bronchitis virus (ibv) were isolated from diseased chickens at different farms in southern china during 2011-2012, and 66.1 % of the isolated strains were associated with typical nephritis. analysis of the s1 gene sequences amplified from the 62 isolated strains together with 40 reference strains published in genbank showed nucleotide homologies ranging from 63.5 to 99.9 % and amino acid homologies ranging from 57.9 to 100 %. phylogenetic analysis revealed t ... | 2014 | 25000989 |
| cpg-odns induced changes in cytokine/chemokines genes expression associated with suppression of infectious bronchitis virus replication in chicken lungs. | the process of virus replication in host cells is greatly influenced by the set of cytokines, chemokines and antiviral substances activated as a result of host-virus interaction. alteration of cytokines profiles through manipulation of the innate immune system by innate immune stimulants may be helpful in inhibiting virus replication in otherwise permissive cells. the aim of present studies was to characterize innate immune responses capable of inhibiting infectious bronchitis virus (ibv) replic ... | 2014 | 25012000 |
| simultaneous typing of nine avian respiratory pathogens using a novel gexp analyzer-based multiplex pcr assay. | a new, rapid, and high-throughput genomelab gene expression profiler (gexp) analyzer-based multiplex pcr method was developed for simultaneous detection and differentiation of nine avian respiratory pathogens. the respiratory pathogens included in this study were avian influenza subtypes h5, h7, and h9, infectious bronchitis virus (ibv), newcastle disease virus (ndv), infectious laryngotracheitis virus (iltv), mycoplasma gallisepticum (mg), mycoplasma synoviae (ms) and haemophilus paragallinarum ... | 2014 | 25025815 |
| regulation of coronaviral poly(a) tail length during infection is not coronavirus species- or host cell-specific. | it has been demonstrated that the length of the poly(a) tail in the bovine coronavirus (bcov), which belongs to genus betacoronaviruses, is regulated throughout infection in human rectal tumor-18 (hrt-18) cells, and the length of the poly(a) tail is associated with the efficiency of virus translation. here, we examined whether the regulation of viral poly(a) tail length is cell-type independent and whether it is a common feature of coronaviruses to assess the significance of the regulation. by l ... | 2014 | 25034371 |
| immune response of broiler chickens fed diets supplemented with different level of chromium methionine under heat stress conditions. | the objectives of this study were to investigate the immune responses of broiler chickens fed diets supplemented with different level of chromium methionine (crmet) in heat stress (hs) condition. two hundred and eighty eight male broiler chickens (ross 308) were allocated to four treatment groups (supplementation with 0, 200, 400 or 800 ppb cr in the form of crmet) in a completely randomized design. the experiment was conducted at heat stressed condition and all birds were kept under temperature ... | 2012 | 25049559 |
| s1 of distinct ibv population expressed from recombinant adenovirus confers protection against challenge. | protective properties of three distinct infectious bronchitis virus (ibv) ark delmarva poultry industry (arkdpi) s1 proteins encoded from replication-defective recombinant adenovirus vectors were investigated. using a suboptimal dose of each recombinant virus, we demonstrated that ibv s1 amino acid sequences showing > or = 95.8% amino acid identity to the s1 of the challenge strain differed in their ability at conferring protection. indeed, the s1 sequence of the ibv population previously design ... | 2014 | 25055623 |
| immunoglobulin a as an early humoral responder after mucosal avian coronavirus vaccination. | infectious bronchitis virus (ibv) is a highly contagious coronavirus prevalent in all countries with an extensive poultry industry and continues to cause economic losses. ibv strains of the ark serotype are highly prevalent in the southeastern united states despite extensive vaccination. one explanation for this observation is the high genetic variability of ibv. in addition, ibv ark-type vaccines may induce suboptimal mucosal immune responses, contributing to the prevalence and persistence of t ... | 2014 | 25055633 |
| varying antibody responses of laying hens housed in an aviary system and in furnished cages. | antibody titers after vaccination against infectious bronchitis virus (ibv), newcastle disease virus (ndv) and after natural infection with campylobacter were analyzed in five trials with lohmann silver laying hens kept in two different housing systems. in these studies it could be demonstrated that antibodies against ibv and campylobacter were in 4 out of 5 respectively in 2 out of 5 trials significantly higher in hens housed in an aviary system compared to those kept in furnished cages. the op ... | 2014 | 25080819 |
| inflammatory and cell-mediated immune responses in the respiratory tract of chickens to infection with avian infectious bronchitis virus. | tracheal mucosa is the primary site of replication of avian infectious bronchitis virus (ibv), which leads to both morphologic and immune modulatory changes in this organ. to increase the understanding of the mechanisms involved in these processes, we focused on the evaluation of local inflammatory and cell-mediated immune responses after challenge with the m41 strain of ibv, associating these responses with pathologic changes in the tracheal mucosa. at 24 h post-infection, inflammatory cytokine ... | 2014 | 25105981 |
| the endoplasmic reticulum stress sensor ire1α protects cells from apoptosis induced by the coronavirus infectious bronchitis virus. | the unfolded-protein response (upr) is a signal transduction cascade triggered by perturbation of the homeostasis of the endoplasmic reticulum (er). upr resolves er stress by activating a cascade of cellular responses, including the induction of molecular chaperones, translational attenuation, er-associated degradation, and other mechanisms. under prolonged and irremediable er stress, however, the upr can also trigger apoptosis. here, we report that in cells infected with the avian coronavirus i ... | 2014 | 25142592 |
| molecular characterization of an infectious bronchitis virus strain isolated from northern china in 2012. | this study reports the complete genome sequence of an infectious bronchitis virus (ck/ch/sd/121220, kj128295) isolated in 2012 from shandong province in northern china. the genome is 27,666 nt long, comprising six genes and 5' and 3' untranslated regions. the full-length genome of the ck/ch/sd/121220 isolate had the highest nucleotide sequence identity (96.7 %) to the yx10 strain. sites of recombination were identified in the genes 1ab, s, 5a, 5b and n, with their putative parental strains belon ... | 2014 | 25168045 |
| recombinant nucleocapsid protein based single serum dilution elisa for the detection of antibodies to infectious bronchitis virus in poultry. | avian infectious bronchitis is ubiquitous and highly contagious disease of poultry, with profound effect on commercial poultry production. for effective control of infectious bronchitis virus (ibv), quick and specific diagnosis is of utmost importance. in this study, the virus was isolated from clinical samples from india and the full length nucleocapsid (n) gene was amplified, cloned and expressed in a prokaryotic system. the purified recombinant n protein based single serum dilution enzyme lin ... | 2014 | 25173423 |
| genomic characteristics and changes of avian infectious bronchitis virus strain ck/ch/ldl/97i after serial passages in chicken embryos. | we previously attenuated the infectious bronchitis virus (ibv) strain ck/ch/ldl/97i and found that it can convey protection against the homologous pathogenic virus. | 2014 | 25195733 |
| development of slide elisa (selisa) for detection of four poultry viral pathogens by direct heat fixation of viruses on glass slides. | the development of an easy and simpler method of slide enzyme-linked immunosorbent assay (selisa) for the diagnosis of four economically important poultry viruses viz., newcastle disease virus (ndv), infectious bronchitis virus (ibv), infectious bursal disease virus (ibdv) and egg drop syndrome 76 virus (eds 76) and the use of selisa for semi quantitation of ndv are described. the positive signals for viral aggregates were detected under light microscope. this is the first report regarding the d ... | 2014 | 25218174 |
| the genotyping of infectious bronchitis virus in taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction. | infectious bronchitis virus (ibv; avian coronavirus) causes acute respiratory and reproductive and urogenital diseases in chickens. following sequence alignment of ibv strains, a combination of selective primer sets was designed to individually amplify the ibv wild-type and vaccine strains using a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction (arms rt-pcr) approach. this system was shown to discriminate the ibv wild-type and vaccine strains. m ... | 2014 | 25223342 |
| an impedance immunosensor based on low-cost microelectrodes and specific monoclonal antibodies for rapid detection of avian influenza virus h5n1 in chicken swabs. | early screening of suspected cases is the key to control the spread of avian influenza (ai) h5n1. in our previous studies, an impedance biosensor with an interdigitated array microelectrode based biochip was developed and validated with pure ai h5 virus, but had limitations in cost and reliability of the biochip, specificity of the antibody against asian in-field h5n1 virus and detection of h5n1 virus in real samples. the purpose of this study is to develop a low-cost impedance immunosensor for ... | 2015 | 25263315 |
| [complete genomic analysis of a novel infectious bronchitis virus isolate]. | the genome of ck/ch/sd09/005, an isolate of infectious bronchitis virus (ibv), was characterized to enable the further understanding of the epidemiology and evolution of ibv in china. twenty-five pairs of primers were designed to amplify the full-length genome of ck/ch/sd09/005. the nucleotide sequence of ck/ch/sd09/005 was compared with reference ibv strains retrieved from genbank. the phylogenic relationship between ck/ch/sd09/005 and the reference strains was analyzed based on s1 gene sequenc ... | 2014 | 25272584 |