Publications

cloning and characterization of the 5' end (exon 1) of the gene encoding human factor x.	human blood-coagulation factor x (hbcfx) is a serine protease zymogen which participates in the middle phase of blood coagulation. recently, we and others have reported the cdna sequences. at present, partial hbcfx gene structure is available. in this paper, we report the isolation of two genomic clones, the x-emb lambda phage clone encoding exons 1 and 2 of the hbcfx gene, and the x-cos cosmid clone encoding exons 2-8. the restriction map of the hbcfx region spans 55 kb. the gene itself was fou ...	1989	2612918
[the role of divalent cations in dna endonucleolysis catalyzed by restrictases].	electrophoretic analysis of products obtained after hydrolysis of phage lambda dna by means of restrictase pae i was carried out after preincubation of dna or the enzyme with mg2+ as well as after preincubation of dna simultaneously with the enzyme and the subsequent addition of the required components into the experimental samples. the analysis showed that mg2+ were apparently not required for the enzyme-substrate complex formation and caused destabilization of the complex. restrictase pae i wa ...	1989	2629242
construction of chickpea genomic library.	for construction of chickpea genomic library, dna was isolated, purified on cscl gradient and size fractionated into 15-20 kb fragments after restriction with sau 3a. these fragments were ligated to phage lambda (embl-3) vector and the recombinant molecules packaged in vitro into viable phage particles. the recombinant phages were obtained as phages on a p2 lysogen of e. coli (spi- selection) and amplified to establish a permanent library. this is the first report of the construction of chickpea ...	1989	2635143
multiple pathways for repair of hydrogen peroxide-induced dna damage in escherichia coli.	the repair response of escherichia coli to hydrogen peroxide has been examined in mutants which show increased sensitivity to this agent. four mutants were found to show increased in vivo sensitivity to hydrogen peroxide compared with wild type. these mutants, in order of increasing sensitivity, were reca, polc, xtha, and pola. the pola mutants were the most sensitive, implying that dna polymerase i is required for any repair of hydrogen peroxide damage. measurement of repair synthesis after hyd ...	1989	2644241
expression of human ferredoxin and assembly of the [2fe-2s] center in escherichia coli.	a cdna fragment encoding human ferredoxin, a mitochondrial [2fe-2s] protein, was introduced into escherichia coli by using an expression vector based on the approach of nagai and thøgersen [nagai, k. & thøgersen, m. c. (1984) nature (london) 309, 810-812]. expression was under control of the lambda pl promoter and resulted in production of ferredoxin as a cleavable fusion protein with an amino-terminal fragment derived from bacteriophage lambda cii protein. the fusion protein was isolated from t ...	1989	2644647
modulation of stability of the escherichia coli heat shock regulatory factor sigma.	the heat shock response of escherichia coli is under the positive control of the sigma 32 protein (the product of the rpoh gene). we found that overproduction of the sigma 32 protein led to concomitant overproduction of the heat shock proteins, suggesting that the intracellular sigma 32 levels limit heat shock gene expression. in support of this idea, the intracellular half-life of the sigma 32 protein synthesized from a multicopy plasmid was found to be extremely short, e.g., less than 1 min at ...	1989	2646289
new reca mutations that dissociate the various reca protein activities in escherichia coli provide evidence for an additional role for reca protein in uv mutagenesis.	to isolate strains with new reca mutations that differentially affect reca protein functions, we mutagenized in vitro the reca gene carried by plasmid mini-f and then introduced the mini-f-reca plasmid into a delta reca host that was lysogenic for prophage phi 80 and carried a lac duplication. by scoring prophage induction and recombination of the lac duplication, we isolated new reca mutations. a strain carrying mutation reca1734 (arg-243 changed to leu) was found to be deficient in phi 80 indu ...	1989	2651400
overexpression of n antitermination proteins of bacteriophages lambda, 21, and p22: loss of n protein specificity.	the n protein of bacteriophage lambda (n lambda) modifies escherichia coli rna polymerase in such a way that it transcribes through termination signals, a process called antitermination. n antitermination normally occurs only if the template contains a specific utilization or nut site upstream of the terminators and only in the presence of host-encoded nus proteins. the lambda-related phages 21 and p22 produce n analogs, n21 and n22, but these require different nut sites and show a different pat ...	1989	2651405
the heat-shock-regulated grpe gene of escherichia coli is required for bacterial growth at all temperatures but is dispensable in certain mutant backgrounds.	previous work has established that the grpe+ gene product is a heat shock protein that is essential for bacteriophage lambda growth at all temperatures and for escherichia coli growth at temperatures above 43 degrees c. here it is shown that the grpe+ gene product is essential for bacterial viability at all temperatures. the strategy required constructing a grpe deletion derivative carrying a selectable chloramphenicol drug resistance marker provided by an omega insertion and showing that this d ...	1989	2651417
mycobacteriophage vector systems.	successful application of molecular genetic approaches to the study of mycobacteria necessitates the introduction of recombinant dna molecules into mycobacterial cells. efficient methods of introducing dna into mycobacterium smegmatis protoplasts have been developed, and the construction of mycobacteriophage recombinant dna vectors has been initiated. novel escherichia coli-mycobacterium shuttle vectors, termed shuttle phasmids, have been constructed. these vectors were constructed by inserting ...	1989	2652256
compilation of dna sequences of escherichia coli.	we have compiled the dna sequence data for e. coli k12 available from the genbank and embo databases and over a period of several years independently from the literature. we have introduced all available genetic map data and have arranged the sequences accordingly. as far as possible the overlaps are deleted and a total of 940,449 individual bp is found to be determined till the beginning of 1989. this corresponds to a total of 19.92% of the entire e. coli chromosome consisting of about 4,720 kb ...	1989	2654890
structure and expression of an alpha-tubulin gene of physarum polycephalum.	fragments of physarum polycephalum dna generated by partial digestion with sau3a were cloned into phage-lambda embl4. a recombinant (phage-lambda e alpha tu) containing an alpha-tubulin (e alpha-tubulin) gene was isolated. the e alpha-tubulin gene is part of the alt b locus. the gene was sequenced and was found to contain seven intervening sequences. the alpha-tubulin isotype (e alpha-tubulin) encoded by the gene has a methionine residue at the c-terminus. the e alpha-tubulin gene has much in co ...	1989	2659344
characterization and vaccine potential of a novel recombinant coccidial antigen.	a cdna clone derived from sporulated oocysts of eimeria tenella and encoding the expression product gx3262 was identified using a monoclonal antibody raised against eimeria acervulina sporozoites. the cdna fragment containing the coccidial antigen gene was cloned in bacteriophage lambda gt11, transferred to a plasmid, and introduced into escherichia coli for analysis of the gene products. the strain carrying the plasmid produced gx3262 as part of a fusion protein consisting of the first 1,006 am ...	1989	2659532
cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of escherichia coli k-12.	the bacterioferritin (bfr) of escherichia coli k-12 is an iron-storage hemoprotein, previously identified as cytochrome b1. the bacterioferritin gene (bfr) has been cloned, sequenced, and located in the e. coli linkage map. initially a gene fusion encoding a bfr-lambda hybrid protein (mr 21,000) was detected by immunoscreening a lambda gene bank containing sau3a restriction fragments of e. coli dna. the bfr gene was mapped to 73 min (the str-spc region) in the physical map of the e. coli chromos ...	1989	2661540
genetic analysis of the lytic replicon of bacteriophage p1. ii. organization of replicon elements.	the region of bacteriophage p1 dna containing a lytic (vegetative) replicon has been identified by cloning p1 fragments into a phage lambda vector. we present the sequence of that replicon. using a novel fusion vector containing two p1 loxp recombination sites, we have developed a transformation assay for replicon function and have used that assay to identify some of the components of the p1 lytic replicon. among those components is a transcription promoter, p53, whose activity is essential for ...	1989	2661830
overproduction and purification of treponema pallidum recombinant-dna-derived proteins tmpa and tmpb and their potential use in serodiagnosis of syphilis.	we report the construction of expression plasmids carrying two treponema pallidum genes encoding for the 42-kilodalton membrane protein tmpa (treponemal membrane protein a) and the 34-kilodalton membrane protein tmpb. using the leftward promoter of bacteriophage lambda, which is controlled by a thermosensitive repressor, we obtained a high level of heat-inducible synthesis of tmpa and tmpb in escherichia coli k-12. both proteins were purified to near homogeneity, and the presence of antibodies t ...	1989	2668179
altered expression of the bacteriophage t4 gene 41 (primase-helicase) in an escherichia coli rho mutant.	bacteriophage t4 gene 41 protein is an essential replication protein, part of the primase-helicase required for lagging strand dna synthesis. in a t4+ infection, 41 rna is first expressed as a polycistronic transcript attached to the upstream rna of genes uvsx (recombination protein) and 40 (stimulates head formation (hinton, d. m. (1989) j. biol. chem. 264, 14432-14439). as infection proceeds, less of the upstream rna extends into gene 41 due to an rna 3' end, approximately equal to 60 bases do ...	1989	2668290
expression of a human liver cytochrome p-450 protein with tolbutamide hydroxylase activity in saccharomyces cerevisiae.	the human liver cytochrome p-450 (p-450) proteins responsible for catalyzing the oxidation of mephenytoin, tolbutamide, and hexobarbital are encoded by a multigene family (cyp2c). although several cdna clones and proteins related to this "p-450mp" family have been isolated, assignment of specific catalytic activities remains uncertain. sulfaphenazole was found to inhibit tolbutamide hydroxylation to a greater extent than mephenytoin or hexobarbital hydroxylation. the inhibition by sulfaphenazole ...	1989	2669966
high-level expression of human dihydropteridine reductase (ec 1.6.99.7), without n-terminal amino acid protection, in escherichia coli.	the cdna coding for human dihydropteridine reductase [dahl, hutchinson, mcadam, wake, morgan & cotton (1987) nucleic acids res. 15, 1921-1936] was inserted downstream of tandem bacteriophage lambda pr and pl promoters in escherichia coli vector pce30. since pce30 also expresses the lambda c1857ts gene, transcription may be controlled by variation of temperature. the recombinant plasmid in an e. coli k12 strain grown at 30 degrees c, then at 45 degrees c, directed the synthesis of dihydropteridin ...	1989	2673215
isolation and structural analysis of the mouse beta-casein gene.	three overlapping clones containing the entire beta-casein gene were isolated from a mouse genomic library constructed in bacteriophage lambda embl3. within the three clones the 6.8-kb casein gene was flanked by about 6-kb (5') and about 10-kb (3') sequences. the complete nucleotide sequence of the gene and its immediate flanking regions was determined. the gene consisted of nine exons ranging from 21 bp to 525 bp separated by introns ranging from 81 bp to 1288 bp. the length of the first exon w ...	1989	2673924
purification of human interleukin-4 produced in escherichia coli.	an interleukin-4 (il4)-encoding cdna isolated from human splenocytes was used to construct an expression plasmid that directs a high-level synthesis of mature il4 protein in escherichia coli. the expression was under the control of the major leftward promoter, pl, of phage lambda and the phage mu ribosome-binding site. the il4 protein was present as insoluble inclusion bodies in the bacterial extract. the il4 could be solubilized in 5 m mgcl2 and was purified to homogeneity by several chromatogr ...	1989	2676727
use of yeast artificial chromosome clones for mapping and walking within human chromosome segment 18q21.3.	well-characterized large genomic clones obtained from yeast artificial chromosome (yac) libraries provide the framework to localize genes and approach genetic disease. we developed universally applicable approaches to establish authenticity, localize and orient internal genes, map restriction sites, and rescue the distal ends of large human genomic dna inserts. we selected human chromosome segment 18q21.3 as a model system. molecular cloning of this segment was initiated by characterizing three ...	1989	2678105
characterization of recombinant thioesterase and acyl carrier protein domains of chicken fatty acid synthase expressed in escherichia coli.	fatty acid synthase of animal tissue is a multifunctional enzyme comprised of two identical subunits, each containing seven partial activities and a site for the prosthetic group, 4'-phosphopantetheine (acyl carrier protein). we have recently isolated cdna clones of chicken fatty acid synthase coding for the dehydratase, enoyl reductase, beta-ketoacyl reductase, acyl carrier protein, and thioesterase domains (chirala, s.s., kasturi, r., pazirandeh, m., stolow, d.t., huang, w.y., and wakil, s.j. ...	1989	2681189
nucleotide sequences of genes encoding heat-stable and heat-labile glyceraldehyde-3-phosphate dehydrogenases; amino acid sequence and protein thermostability.	the structural gene (gapst) encoding glyceraldehyde-3-phosphate dehydrogenase (gpdh; ec 1.2.1.12) from bacillus stearothermophilus has been cloned in escherichia coli using plasmid pbr322 as a vector; the homologous gene (gapco) from bacillus coagulans was cloned from a phage lambda library. expression of the cloned gap genes revealed that, as in the wild-type (wt) organisms, the gpdh from b. stearothermophilus (gpdh-st) was intrinsically heat stable (hs) and that from b. coagulans (gpdh-co) hea ...	1989	2684782
cloning, sequencing and expression of the l-2-hydroxyisocaproate dehydrogenase-encoding gene of lactobacillus confusus in escherichia coli.	the gene (l-hicdh) encoding l-2-hydroxyisocaproate dehydrogenase (l-hicdh) from lactobacillus confusus was cloned in escherichia coli. a 69-mer oligodeoxyribonucleotide probe, derived to be complementary to the n-terminal amino acid (aa) coding sequence, was used for screening. the complete nucleotide (nt) sequence of the l-hicdh gene was determined. the 5'-end of the mrna was mapped by primer extension and the promoter identified. downstream from the l-hicdh gene is a typical rho-independent te ...	1989	2684788
sequence analysis of the ura1 gene encoding orotidine-5'-monophosphate decarboxylase of schizophyllum commune.	the ura1 gene (encoding orotidine-5'-monophosphate decarboxylase) of the basidiomycete fungus schizophyllum commune was mapped to a 1.4-kb bgli-bamhi fragment of two independent phage lambda clones previously isolated from a schizophyllum genomic library. the fragment was identified by its ability to complement schizophyllum ura1 mutants via transformation. the complete nucleotide sequence of the fragment containing the ura1 gene was determined. sequence analysis revealed that the coding region ...	1989	2684794
the major 45-kda protein of the yeast mitochondrial outer membrane is not essential for cell growth or mitochondrial function.	as part of an analysis of the function and assembly of the mitochondrial outer membrane, we have cloned and characterized the yeast gene encoding a 45-kda polypeptide (om45) which is a major constituent of this membrane. the nuclear gene was isolated by immunological screening of plaques of recombinant phage lambda gt11 containing fragments of yeast genomic dna using an antibody against om45. determination of the nucleotide sequence of the dna fragment isolated by this approach revealed a single ...	1989	2687271
multiple harvey-ras genes in the bovine genome.	three different harvey-ras (ha-ras) genes have been identified in the bovine genome by screening a lambda phage-bovine dna library with the ras gene of the harvey murine sarcoma virus. the genes have been characterized by hybridization and heteroduplex analysis with both the viral and human ha-ras genes. based on heteroduplex mapping, the putative direction of transcription and the approximate position of the coding regions of the bovine genes were established. two genes, bovine ha-ras 1 and 2, ...	1989	2687766
molecular and functional characterization of amylin, a peptide associated with type 2 diabetes mellitus.	the 37-amino acid peptide called amylin is a major component of the islet amyloid deposited in the pancreases of persons with type 2 diabetes mellitus. we report the isolation of a partial cdna clone and a phage lambda genomic clone of the coding region of the amylin gene. the dna sequence encodes a protein sequence identical to that of amylin isolated from the amyloid found in the diabetic pancreas and shows that amylin is likely to be synthesized as a precursor peptide, now named proamylin. we ...	1989	2690069
cysteine-22 and cysteine-38 are not essential for the functions of maltoporin (lamb protein).	maltoporin in the outer membrane of escherichia coli contains two cysteine residues, at positions 22 and 38 in the primary sequence. the role of these residues in determining structural stability, and their contributions to the maltoporin binding sites for maltodextrins and bacteriophage lambda, was investigated. site-directed mutagenesis was used to alter each of these residues to a serine. a double mutant lacking both cysteines was also isolated. none of the substitutions affected maltodextrin ...	1989	2693195
expression in escherichia coli of fragments of glial fibrillary acidic protein: characterization, assembly properties and paracrystal formation.	we have expressed in escherichia coli a 1258 bp cdna fragment corresponding to 97% of mouse glial fibrillary acidic protein (gfap), the principal intermediate filament protein of astrocytes. high levels of expression were obtained, as a fusion protein with 32 residues of the bacteriophage lambda cii protein, using the plcii expression vector system of k. nagai and h.-c. thogersen. although removal of the cii protein fragment by proteolysis using factor x proved difficult, a protein corresponding ...	1989	2693466
the location of a temperature-sensitive trans-dominant mutation and its effect on restriction and modification in escherichia coli k12.	an escherichia coli k12 chromosomal ecori-bamhi fragment containing a mutant hsds locus was cloned into plasmid pbr322. the mcrb gene, closely linked to hsds, was used for selection of clones with the inserted fragment using t4 alpha gt57 beta gt14 and lambda vir. pvuii phages; the phage dnas contain methylated cytosines and hence can be used to demonstrate mcrb restriction. for the efficient expression of the hsds gene, a bglii fragment of phage lambda carrying the pr promoter was inserted into ...	1989	2693592
the p2 phage old gene: sequence, transcription and translational control.	the old (overcoming lysogenization defect) gene product of bacteriophage p2 kills escherichia coli recb and recc mutants and interferes with phage lambda growth [sironi et al., virology 46 (1971) 387-396; lindahl et al., proc. natl. acad. sci. usa 66 (1970) 587-594]. specialized transducing lambda phages, which lack the recombination region, can be selected by plating lambda stocks on e. coli that carry the old gene on a prophage or plasmid [finkel et al., gene 46 (1986) 65-69]. deletion and seq ...	1989	2695400
structural organization of the gene for the alpha 1 chain of human type iv collagen.	the complete exon size and distribution pattern in the gene for the alpha 1 chain of human type iv collagen was determined. clones covering 145 kilobases (kb) of genomic dna including 100 kb of the gene itself as well as 25 kb upstream and 20 kb downstream of the gene sequences, respectively, were isolated from lambda phage and cosmid libraries. the overall gene structure was determined by endonuclease restriction mapping and r-loop analyses and all exon sizes by nucleotide sequencing. the chara ...	1989	2701944
olfactory marker protein gene: its structure and olfactory neuron-specific expression in transgenic mice.	olfactory marker protein (omp) genomic clones were isolated from a charon 4a phage lambda rat genomic library. a 16.5-kilobase (kb) fragment of the rat genome containing the gene was isolated and characterized. sequence analysis of the gene showed the absence of introns and the lack of caat and tata boxes in the 5' flanking region. the transcription initiation site was mapped, and two sites 55 and 58 base pairs upstream of the atg were observed. the 5' flanking region is rich in g+c residues and ...	1989	2701951
stochastic models for heterogeneous dna sequences.	the composition of naturally occurring dna sequences is often strikingly heterogeneous. in this paper, the dna sequence is viewed as a stochastic process with local compositional properties determined by the states of a hidden markov chain. the model used is a discrete-state, discrete-outcome version of a general model for non-stationary time series proposed by kitagawa (1987). a smoothing algorithm is described which can be used to reconstruct the hidden process and produce graphic displays of ...	1989	2706403
a repeated segment on the mouse y chromosome is composed of retroviral-related, y-enriched and y-specific sequences.	we report the isolation and characterization of two recombinant clones containing dna derived from the y chromosome of the c57bl/10 inbred mouse strain. both clones were isolated from a lambda phage library derived from a partial ecori digest of c57bl/10 male dna using the murine retrovirus m720. characterization of these clones showed they were derived from a repeated segment present on the c57bl/10j y chromosome that contains sequences found elsewhere in the genome. in addition, one clone cont ...	1989	2731728
characterization of cdnas encoding human pyruvate dehydrogenase alpha subunit.	a cdna clone (1423 base pairs) comprising the entire coding region of the precursor form of the alpha subunit of pyruvate dehydrogenase (e1 alpha) has been isolated from a human liver cdna library in phage lambda gt11. the first 29 amino acids deduced from the open reading frame correspond to a typical mitochondrial targeting leader sequence. the remaining 361 amino acids, starting at the n terminus with phenylalanine, represent the mature mitochondrial e1 alpha peptide. the cdna has 43 base pai ...	1989	2748588
[regulation of the synthesis of total cellular proteins and monoclonal antibodies in a hybridoma cell culture].	a study was made of the regulation of total protein synthesis in cells of the mouse hybridoma producing monoclonal antibodies (mcab) against lambda phage, and in the course of hybridoma growth and at the change of fetal bovine serum (fbs) concentration. fbs strictly affected proliferation of hybridoma cells, the specific production of mcab per cell being unchanged. the rate of total cellular protein synthesis does depend on fbs concentration in the medium, whereas the rates of protein degradatio ...	1989	2749901
nick translation of dna immobilized on nylon membranes.	nick translation of dna bound to nylon membranes is described. phage lambda dna was digested with restriction endonuclease hindiii. the fragments were separated by agarose electrophoresis and electrophoretically transferred to zeta-probe nylon membranes. after being air-dried, the areas with dna fragments attached were cut out and subjected to nick translation. the labeled fragments, removed from the membranes by a single wash step, can be used as specific hybridization probes. currently used me ...	1989	2751087
localization in man of fifteen dna sequences within the chromosome segment 13q12-q22.	fifteen human chromosome 13 specific dna fragments, isolated from a lambda phage genomic library, were localized within the segment 13q12-q22. one was mapped to 13q12.1-q12.2, three to 13q12.3-q13.1, one to 13q14,1-q14.2, five to 13q14.1-q21.1, one to 13q21.1-q21.2, two to 13q21.2, and one to 13q22.1, and one to 13q22. the localization was performed by hybridization to southern blots of a panel of human cell lines with overlapping deletions in 13q, and for three probes also by in situ hybridizat ...	1989	2753742
paramyosin gene (unc-15) of caenorhabditis elegans. molecular cloning, nucleotide sequence and models for thick filament structure.	paramyosin is a major structural component of thick filaments isolated from many invertebrate muscles. the caenorhabditis elegans paramyosin gene (unc-15) was identified by screening with specific antibodies an "exon-expression" library containing lacz/nematode gene fusions. short probes recovered from the library were used to identify bacteriophage lambda and cosmid clones that encompass the entire paramyosin (unc-15) gene. from these clones, numerous subclones containing epitopes reacting with ...	1989	2754728
molecular cloning of genes from ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities.	clones expressing activity against xylan or beta(1-3,1-4)glucan (lichenan) were isolated from a library of ruminococcus flavefaciens 17 dna made in bacteriophage lambda embl3. hybridization analyses indicated the recovery of four separate genes encoding xylanases that showed no detectable associated carboxylmethylcellulase activity. one of these genes was associated with clones that also expressed beta(1-3,1-4)glucanase and beta-xylosidase activities.	1989	2757382
nucleotide sequence of the bacteriophage p22 gene 19 to 3 region: identification of a new gene required for lysis.	the nucleotide sequence of a 2558-bp region of bacteriophage p22 at the right end of the genetic map between genes 19 and 3 was determined. a new gene that is partially required for lytic growth, named gene 15, was noted. p22 mutants were constructed which lack gene 15 function, and the gene 15 product was found to be required for lysis in the presence of some divalent cations. it has extensive amino acid sequence similarity with the phage lambda rz gene, which has a similar function, and weak s ...	1989	2763468
human platelet glycoprotein ix: an adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures.	the glycoprotein (gp) ib-ix complex on the surface of human platelets functions as the von willebrand factor receptor and mediates von willebrand factor-dependent platelet adhesion to blood vessels. gpix is a relatively small (mr, 17,000) protein that may provide for membrane insertion and orientation of the larger component of the complex, gpib (mr, 165,000). using antibody screening, we cloned a cdna encoding gpix from a human erythroleukemia cell cdna library constructed in phage lambda gt11. ...	1989	2771955
identification of the initiation codons for translation of cowpea mosaic virus middle component rna using site-directed mutagenesis of an infectious cdna clone.	a full-length cdna copy of cpmv m rna has been cloned downstream of a phage lambda promoter in the plasmid ppmi. transcripts obtained from this clone can be translated in vitro and replicated in cowpea mesophyll protoplasts in the presence of viral b rna. we have constructed a series of site-directed mutants of this clone to investigate the mechanism of translation of cpmv m rna. the results obtained confirm that the aug at position 161 is used to direct the synthesis of a 105k protein in vitro ...	1989	2773321
two haemophilus influenzae rd genes that complement the reca-like mutation rec-1.	two haemophilus influenzae rd genes each complemented the pleiotropic defects of the reca-like mutation rec-1. one gene, fec, was isolated on a 3.6-kilobase-pair ecori restriction fragment by complementation of the fec- phenotype of bacteriophage lambda. the other gene, rec, was identified on a 3.1-kilobase-pair ecori fragment by southern hybridization by using reca-like gene probes from erwinia carotovora and pseudomonas aeruginosa pao. in a rec-1 strain of h. influenzae, the cloned genes resto ...	1989	2785104
highly sensitive biotin-labelled hybridization probe.	a simple chemical method for introducing biotin into nucleic acids has been developed for the synthesis of nonisotopic hybridization probes. the method is based on the reaction of biotin hydrazide with amino residues of nucleic acids by using glutaraldehyde as a bifunctional coupling reagent. biotin-labelled deoxyribonucleic acid (dna) was detected by the use of alkaline phosphatase-labelled avidin, and alkaline phosphatase activity was measured by colorimetric and chemiluminescence methods. the ...	1989	2805162
isolation of a cdna encoding the rat liver s-adenosylmethionine synthetase.	we have isolated cdna clones encoding the rat liver s-adenosylmethionine synthetase by means of immunological screening from a phage lambda gt 11 expression library containing cdna synthesized from adult rat liver poly(a)-rna. the amino acid sequence deduced from the cdna indicates that the rat liver enzyme for this protein contains 397 amino acid residues and has a molecular mass of 43697 da. the deduced amino acid sequence of rat liver s-adenosylmethionine synthetase was 68% similar to those o ...	1989	2806235
cloning and expression of an avian metallothionein-encoding gene.	the chicken metallothionein gene (cmt), isolated from a chicken genomic dna phage lambda library, was found to be approximately 1.5 kb in length and to consist of three exons, separated by two intervening sequences. the number and placement of the introns in the cmt gene is precisely the same as that in the mammalian metallothionein-coding genes. s1 nuclease mapping indicated a prominent transcription start point (tsp) 62 bp 5' to the translation start codon. the promoter region analyzed (623 bp ...	1989	2806910
bacteriophage t4 early promoter regions. consensus sequences of promoters and ribosome-binding sites.	twenty-nine early promoters from bacteriophage t4 and 14 early promoters from bacteriophage t6 were isolated using vector m13hdl17, a promoterless derivative of m13mp8 carrying a linker sequence, the bacteriophage lambda-terminator tr1, and the lacz' gene including part of its ribosome-binding site. the consensus sequence for the t4 promoters is: (sequence; see text). ribosome-binding sites of t4 share the sequence: 5'...g.ggaga..aa.atgaa.a...3' the consensus sequence of the t4 early promoter re ...	1989	2810355
isolation and characterization of the human uracil dna glycosylase gene.	a series of anti-human placental uracil dna glycosylase monoclonal antibodies was used to screen a human placental cdna library in phage lambda gt11. twenty-seven immunopositive plaques were detected and purified. one clone containing a 1.2-kilobase (kb) human cdna insert was chosen for further study by insertion into puc8. the resultant recombinant plasmid selected by hybridization a human placental mrna that encoded a 37-kda polypeptide. this protein was immunoprecipitated specifically by an a ...	1989	2813420
[mapping the genome locus containing genes for mouse tumor necrosis factor by means of hybridization end labelling with the use of synthetic oligonucleotides].	we have developed a technique for restriction nuclease sites mapping in genomic dna cloned into phage lambda vectors. synthetic oligonucleotides homologous to the vector sequences and adjacent to the cloning site were used as hybridisation probes for indirect end labelling procedure. in addition, a number of oligonucleotides homologous to the sequences of tumour necrosis factor genes were used for mapping from the internal sites. as a result, a map of 35 kb genomic region on the chromosome 17 in ...	1989	2818653
high level expression of the ecop1 modification methylase gene and characterisation of the gene product.	we have cloned the gene coding for the ecop1 modification methylase in an expression system based on the phage lambda pl promoter and the ci857-coded thermoinducible repressor. we have used this system to purify the enzyme on the 20-30-mg scale and have examined some of its enzymatic properties. the enzyme is a tetramer of mr 72,000 subunits and is approx. 40% alpha-helical. experiments with the methyl donor, s-adenosyl methionine, radioactively labelled in different positions indicate that a me ...	1987	2820845
hemophilia b (factor ixseattle 2) due to a single nucleotide deletion in the gene for factor ix.	to understand the molecular basis for hemophilia b in patients with little or no circulating factor ix antigen, a patient who had less than 0.2% circulating factor ix antigen (factor ixseattle 2) was selected for analysis of his factor ix gene. genomic dna fragments from the abnormal gene were cloned into bacteriophage lambda vectors and recombinant phage were identified using radiolabeled genomic probes obtained from the normal factor ix gene. the exons and flanking regions of the abnormal gene ...	1987	2821070
lambda n antitermination system: functional analysis of phage interactions with the host nusa protein.	coliphage lambda gene expression is regulated temporally by systems of termination and antitermination of transcription. the lambda-encoded n protein (pn) acting with host factors (nus) at sites (nut) located downstream from early promoters is the first of these systems to operate during phage development. we report observations on some of the components of this complex system that, in part, address the way in which these elements interact to render rna polymerase termination-resistant. (1) the ...	1987	2821265
nusa protein of escherichia coli is an efficient transcription termination factor for certain terminator sites.	we have studied the factors that affect transcription termination in vitro at the tr2 terminator of bacteriophage lambda and at the t1 terminator of the escherichia coli rrnb operon. termination efficiency at both of these sites is enhanced by the e. coli nusa protein, giving final efficiencies of termination in vitro comparable to those estimated in vivo. transcripts terminated in the presence of nusa protein are all released from the rna polymerase complex, indicating that a complete terminati ...	1987	2821282
[the gene for bovine growth hormone is flanked by homologous sequences].	the gene coding for the bovine growth hormone was isolated from a lambda-phage library. the restriction map of the 3'-region (about 10 kb) was determined by restriction analysis and by southern blot analysis. a comparison of the 5'- and 3'-flanking regions of the bovine growth hormone gene revealed some patches of homology.	1987	2821383
characterization of two solitary long terminal repeats of murine leukemia virus type that are conserved in the chromosome of laboratory inbred mouse strains.	twenty molecular clones containing sequences homologous to the long terminal repeats (ltrs) of the endogenous ecotropic murine leukemia virus (mulv) of the rfm/un mouse were isolated from a library of rfm/un mouse spleen dna in phage lambda. three of these ltrs were not associated with any viral structural genes. nucleotide sequence analysis demonstrated that they were solitary ltrs which were flanked by 4-bp directly repeated cellular sequences and which lacked primer binding sites. two of the ...	1987	2821681
escherichia coli integration host factor bends the dna at the ends of is1 and in an insertion hotspot with multiple ihf binding sites.	the integration host factor of escherichia coli (ihf) is a small, histone-like protein which participates in the integration of bacteriophage lambda into the e. coli chromosome and in a number of regulatory processes. our recent footprinting analysis has shown that ihf binds specifically to the ends of the transposable element is1, as well as to several sites within a short segment of the plasmid pbr322. we have extended our studies of the binding of the ihf molecule to these sites in vitro usin ...	1987	2822395
[camp acceptor protein of escherichia coli k-12 may be the repressor of the late promoter of bacteriophage lambda].	by computer analysis of the lambda phage dna putative camp-cap binding site has been found. this site (44506-acgtgtgaccgcattcaaaat-44486) is located 40 bp upstream to the late pr' promoter. the sequence denoted is located on the coding dna strand, similar to the camp-cap binding sites in all four genes known to be subject to camp-cap repression. it is suggested that camp-cap can serve as a repressor of the phage lambda late promoter pr'. such repression can arrest the expression of late lambda g ...	1987	2822536
two bovine genes for cytochrome c oxidase subunit iv: a processed pseudogene and an expressed gene.	we have isolated and analyzed 17 clones from a bovine genomic library in phage lambda charon28 probed with a bovine liver cdna for cytochrome c oxidase subunit iv. restriction enzyme mapping and southern analysis indicated that these clones represent only two genomic regions. one region was shown by nucleotide sequencing to contain a subunit iv pseudogene of the processed type. the other class of clones contained the 5' region of a putative expressed gene; the region consists of two exons and tw ...	1987	2822541
mutant isolation and molecular cloning of mre genes, which determine cell shape, sensitivity to mecillinam, and amount of penicillin-binding proteins in escherichia coli.	a chromosomal region of escherichia coli contiguous to the fabe gene at 71 min on the chromosomal map contains multiple genes that are responsible for determination of the rod shape and sensitivity to the amidinopenicillin mecillinam. the so-called mre region was cloned and analyzed by complementation of two closely related but distinct e. coli mutants characterized, respectively, by the mutations mre-129 and mre-678, that showed a rounded to irregular cell shape and altered sensitivities to mec ...	1987	2822655
genetic and physical location of the escherichia coli rap locus, which is essential for growth of bacteriophage lambda.	the escherichia coli rap mutant does not support the growth of bacteriophage lambda (d. henderson and j. weil, virology 71:546-559, 1976). we located the rap site at 26 min in the e. coli genetic map and determined the gene order fadr-rap-supf-trp from our transduction experiments. plasmid pho1 harbors a 5.6-kilobase-pair segment of the e. coli chromosome which contains the pth gene (b. hove-jensen, mol. gen. genet. 201:269-276, 1985). this plasmid complemented rap bacteria, suggesting that it c ...	1987	2822668
conservation of delta-crystallin gene structure between ducks and chickens.	a cloned chicken delta-crystallin cdna was used to identify two putative delta-crystallin genes in the duck by southern blot hybridization. a dna fragment containing most of one of these genes was isolated from a library made in bacteriophage lambda charon 28a containing genomic dna from 14-day-old embryonic ducks. electron microscopy, partial gene sequencing, primer extension analysis using duck mrna, and comparison with the well-characterized chicken delta-crystallin genes suggest that our clo ...	1987	2822941
unwinding of duplex dna from the sv40 origin of replication by t antigen.	the t antigen specified by sv40 virus is the only viral-encoded protein required for replication of sv40 dna. t antigen has two activities that appear to be essential for viral dna replication: specific binding to duplex dna at the origin of replication and helicase activity that unwinds the two dna strands. as judged by electron microscopy, dna unwinding is initiated at the origin of replication and proceeds bidirectionally. either linear or circular dna molecules containing the origin of repli ...	1987	2823389
[dna isolated from streptococcus sp. thom-1066--the producer of thomicide].	thomicide is a complex preparation including a bacteriocin-like substance. to localize the determinant responsible for synthesis of the bacteriocin-like substance, dna of the streptococcus producing thomicide was isolated and studied. equilibrium centrifugation of the total dna preparation in the gradient of cesium chloride-ethidium bromide yielded dna of one density. the total dna preparation was obtained with alkaline and neutral lysis. restriction analysis of the streptococcal dna followed by ...	1987	2823692
a genetic system for isolation and characterization of taqi restriction endonuclease mutants.	the gene encoding taqi restriction endonuclease has been subcloned downstream from an inducible phoa promoter. certain strains of escherichia coli remain viable when endonuclease is expressed, even in the absence of (protective) methylation. infecting lambda phage dna is not restricted in vivo. one e. coli strain, mm294, exhibited a temperature-sensitive phenotype when taqi endonuclease was induced. this allowed for design of an in vivo plate assay for identification of specially constructed two ...	1987	2824288
a system for in vivo selection of genomic rearrangements with predetermined endpoints in escherichia coli using modified tn10 transposons.	using recombinant dna techniques, the tn10-specific teta gene (coding for tetracycline resistance) has been mutagenized by insertion of a streptomycin-resistance or a kanamycin-resistance gene. the insertions occurred at loci separated by 920 bp. the mutated teta fragments, respectively designated as tes (for tetracycline-streptomycin) and tek (for tetracycline-kanamycin), were subsequently cloned into a phage lambda ciii+cits857cii+ in replacement of the att lambda region. the two recombinant p ...	1987	2824289
transposition of tn1000: in vivo properties.	transposition mediated by the tn1000 transposase was investigated by using transposon variants carrying synthetic or wild-type termini but no intact tn1000 genes. transposon tn1001, whose only homologies to tn1000 are in its 38-base-pair terminal inverted repeats, transposed at the same rate as tn1005, an artificial construct carrying wild-type tn1000 termini and approximately 1 kilobase of flanking tn1000 dna at each end, when transposase was supplied in trans. the majority of the transposition ...	1987	2824438
a class of escherichia coli proteins controlled by the hfla locus.	the hfla protein of escherichia coli is critical for the choice of the lytic or lysogenic pathway by bacteriophage lambda. to investigate whether hfla plays a regulatory role in e. coli, we used two-dimensional gel electrophoresis to compare the distribution of e. coli proteins in hfla+ and hfla- cells. we found at least 13 proteins that are present in hfla- strains, but absent or very low in hfla+ strains. this observation indicates that hfla might be involved in regulation of a large class of ...	1987	2824788
[study of the structure of phage lambda operator or1 using two-dimensional 1h-nmr-spectroscopy].	noesy spectroscopy at 500 hmz was employed to assign resonances of nonexchangeable protons in the 1h nmr spectrum of the synthesized 17 base pair duplex of deoxyribonucleotides comprising the or1 operator, one of the specific binding sites for the bacteriophage lambda cro repressor. a pure absorption spectrum that was obtained by the phase sensitive detection technique allowed to perform a resonance assignment of base and deoxyribose protons, excepting h-5'- and h-5"-protons, on the basis of a s ...	1987	2824987
genomic organization of low copy number sequences that are associated with deca-satellite dna in the monkey genome.	a previously described segment of african green monkey dna (cloned in phage lambda mka) contains deca-satellite linked to dna sequences that are estimated to occur once per genome. sequences homologous to the low copy number sequences in lambda mka are also associated with species-specific satellite dnas in the human and mouse genomes. a second clone, lambda mk8, contains a monkey dna region that is colinear and homologous to a portion of the low copy number sequences in lambda mka, but no satel ...	1987	2825119
nucleotide sequence of the gene for human prothrombin.	a human genomic dna library was screened for the gene coding for human prothrombin with a cdna coding for the human protein. eighty-one positive lambda phage were identified, and three were chosen for further characterization. these three phage hybridized with 5' and/or 3' probes prepared from the prothrombin cdna. the complete dna sequence of 21 kilobases of the human prothrombin gene was determined and included a 4.9-kilobase region that was previously sequenced. the gene for human prothrombin ...	1987	2825773
phototoxic potential of afloqualone, a quinazolinone derivative, as determined by photosensitized inactivation of bacteriophage.	effect of uv-a irradiation on bacteriophage lambda in the presence of afloqualone (aq) was examined to obtain in vitro evidence for phototoxic potential of aq, a centrally acting muscle relaxant. neither aq itself nor the long-lived photoproducts affected viability of the phage, but the phage was inactivated when it was irradiated in the presence of the drug. photosensitized inactivation was efficiently repressed by the presence of radical scavengers such as hydroquinone, cysteamine and cystein ...	1987	2826022
expression of proteins essential for is1 transposition: specific binding of insa to the ends of is1.	the insertion sequence is1 displays a complex array of open reading frames (orf). in an attempt to identify those which encode polypeptide products, we have systematically placed each orf under the control of the p1 promoter of phage lambda. in the expression system we used, only the product of the insa gene was present in high enough amounts to be detected by polyacrylamide gel electrophoresis. the production of insa was further increased in a first codon hook-up to phage t7 transcriptional and ...	1987	2826132
plasmid and bacteriophage vectors for excision of intact inserts.	plasmid (ppolyiii) and bacteriophage lambda (embl301) vectors are described in which sites for the rare-cutting enzymes sfii and noti (8-bp, recognition sequences) flank the polylinker cloning region. intact dna inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. general-purpose miniplasmid cloning vectors ppolyi and ppolyii are also described, and the utility of the bacteriophage lambda vector is demonstrated in the construction of a bovin ...	1987	2826295
sequence requirements of escherichia coli atttn7, a specific site of transposon tn7 insertion.	transposon tn7 transposes at high frequency to a specific site, atttn7, in the escherichia coli chromosome. we devised a quantitative assay for tn7 transposition in which tn7-end derivatives containing the cis-acting transposition sequences of tn7 transpose from a bacteriophage lambda vector upon infection into cells containing the tn7-encoded transposition proteins. we used this assay to identify a 68-base-pair dna segment containing the sequences essential for atttn7 target activity. this segm ...	1988	2826397
cloning and characterization of the aeromonas caviae reca gene and construction of an a. caviae reca mutant.	a recombinant plasmid carrying the reca gene of aeromonas caviae was isolated from an a. caviae genomic library by complementation of an escherichia coli reca mutant. the plasmid restored resistance to both uv irradiation and to the dna-damaging agent methyl methanesulfonate in the e. coli reca mutant strain. the cloned gene also restored recombination proficiency as measured by the formation of lac+ recombinants from duplicated mutant lacz genes and by the ability to propagate a strain of phage ...	1988	2826405
the grpe protein of escherichia coli. purification and properties.	the grpe gene of escherichia coli was first identified because a mutation in it, grpe280, prevented bacteriophage lambda dna replication in vivo. subsequent work resulted in the identification of the grpe protein in two-dimensional gels and its classification as a heat shock protein. here we report the purification of the grpe protein. we show that overproduction of grpe occurs in dnak 103 bacteria which do not produce a functional mr 72,000 dnak protein. the grpe protein was purified from this ...	1987	2826421
the nul subunit of bacteriophage lambda terminase binds to specific sites in cos dna.	the maturation and packaging of bacteriophage lambda dna are under the control of the multifunctional viral terminase enzyme, which is composed of the protein products of nu1 and a, the two most leftward genes of the phage chromosome. terminase binds selectively to the cohesive end site (cos) of multimeric replicating lambda dna and introduces staggered nicks to regenerate the 12-base single-stranded cohesive ends of the mature phage genome. the purified gpnu1 subunit of terminase forms specific ...	1988	2826803
characterization of the types of mutational events that spontaneously occur in a plasmid system.	the immunity region from a ci857 derivative of bacteriophage lambda has been cloned into the ecori site of pbr322 to produce a plasmid that can be used to analyze spontaneous mutagenesis. cells containing this plasmid are temperature-sensitive for growth unless mutations have occurred that somehow prevent the expression of the kil gene in the lambda fragment at non-permissive temperature. 678 such temperature-resistant mutants from 10 independent subcultures each of 2 different reca- e. coli str ...	1988	2827020
organization of the gene for human factor xi.	factor xi (plasma thromboplastin antecedent) is a plasma glycoprotein that participates in the early phase of blood coagulation. the gene for the human protein has been isolated from two different lambda phage genomic libraries. four independent recombinant lambda phage carrying overlapping dna inserts that coded for the entire gene for factor xi were isolated and characterized by restriction mapping, southern blotting, and selective dna sequencing to establish the number and location of the int ...	1987	2827746
construction and characterization of plasmid and lambda phage vector systems for study of transcriptional control in escherichia coli.	we constructed a family of lambda phage and plasmid vectors which facilitate cloning and quantitative analysis of transcriptional regulator in both single and multiple copies. their expression system was modified from the ara-trp-lac fusion operon of plasmid pmc81 [casadaban and cohen, j. mol. biol. 138 (1980) 179-207], which is designed to assay both promoters and terminators with a single vehicle. to eliminate transcriptional and translational polar effects liable to occur in the original fusi ...	1987	2828183
temporal control of transposition in tn5.	is50r is an insertion sequence associated with the transposon tn5. is50r carries the structural genes for two proteins; one (p1) is the tn5 transposase, and the other (p2) is an inhibitor of transposition. these two proteins are translated from two different transcripts, m1 and m2. when bacteriophage lambda::is50r dna was introduced into a bacterial cell, m1 and m2 were initially at relative levels of about 1 to 2. as time progressed the amount of m1 fell, whereas the amount of m2 continued to i ...	1988	2828332
studies on the arrangement of dna inside viruses using a breakable bis-psoralen crosslinker.	we have developed a new dna-dna crosslinking strategy based on a cleavable bispsoralen reagent and used this strategy to study the structures of bacteriophage lambda and the animal virus sv40. our results show that in both lambda and sv40, all restriction fragments examined can be crosslinked to all other restriction fragments. in bacteriophage lambda, the crosslinking data are consistent with a random packaging model, while in sv40 there is some deviation from the random model. these results im ...	1987	2828635
escherichia coli dnaa initiation function is required for replication of plasmids derived from coliphage lambda.	the dnaa gene function, indispensable for the initiation of escherichia coli replication from oric is not essential for the growth of phage lambda. the in-vitro replication of plasmids derived from phage lambda does not seem to require dnaa protein either. however, we present evidence that in vivo the normal replication of lambda plasmids is dnaa-dependent. after inactivating the dnaa gene function, half of the plasmid molecules may enter a single round of replication. rifampicin sensitivity of ...	1987	2828637
structure of cryptic lambda prophages.	when escherichia coli cells lysogenic for bacteriophage lambda are induced with ultraviolet light, cells carrying cryptic lambda prophages are occasionally found among the apparently cured survivors. the lambda variant crypticogen (lambda crg) carries an insertion of the transposable element is2, which increases the frequency of cryptic lysogens to about 50% of cured cells: 43 of these cryptic prophages have been characterized. they all contain substitutions that replace the early segment of the ...	1987	2828640
lambda phage vectors--embl series.		1987	2828839
organisation of the escherichia coli chromosome between genes glns and glnu, v.	analysis of bacteriophage lambda dna and of subcloned plasmid dna has allowed the localisation of the following genes, located at 16 min on the escherichia coli chromosome, within a restriction map of the region: glns, nage, nagb, naga, asnb, mett, leuw, glnu, glnu, mett; glnv, glnv.	1987	2828887
[effective selection and analysis of is1 element transposition using the olpln region of phage lambda].	the plasmid pnt6 permits selection of is1-element insertions into the plasmid occurring with the frequency about 10%. the bacteriophage promoter pl cloned in pnt6 is a hot-spot region for is1-element insertion. the frequency of is1 transposition into pl depends on genotype. the plasmid pnt6 may be considered to be a useful target dna for screening and analysis of is1-element transposition.	1987	2828939
identification of a cdna encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy.	humoral hypercalcemia of malignancy is a common paraneoplastic syndrome that appears to be mediated in many instances by a parathyroid hormone-like peptide. poly(a)+ rna from a human renal carcinoma associated with this syndrome was enriched by preparative electrophoresis and used to construct an enriched cdna library in phage lambda gt10. the library was screened with a codon-preference oligonucleotide synthesized on the basis of a partial n-terminal amino acid sequence from a human tumor-deriv ...	1988	2829195
molecular cloning of the genome of human spumaretrovirus.	dna of human spumaretrovirus (hsrv) was cloned from both cdna and from viral dna into phage lambda and bacterial plasmid vectors. the recombinant plasmids harboring viral dna were characterized by southern blot hybridization and restriction mapping. physical maps were constructed from cdna and found to be colinear with the restriction maps obtained from viral dna. the recombinant clones isolated contained viral dna inserts which range in size from 2.2 kb to 15.4 kb. the recombinant clones allowe ...	1987	2830164
'illegitimate' recombination events in polyoma-transformed rat cells.	in the lpt line of polyoma (py)-transformed rat cells, amplification of the integrated viral dna and of cell nucleotide sequences flanking the viral integration site, can be induced either spontaneously or by treatment with carcinogens. we show here that the amplified dna includes interspersed viral and cellular sequences generated by 'illegitimate' recombination events. genomic libraries have been prepared in phage lambda vectors from lpt cells treated with the inducing agent mitomycin c and fr ...	1987	2830165
characterization of the azorhizobium sesbaniae ors571 genomic locus encoding nadph-glutamate synthase.	sixteen independent azorhizobium sesbaniae ors571 vector insertion (vi) mutants defective in ammonium assimilation (asm-) were selected; genomic dna sequences flanking the insertion endpoints were cloned directly. resulting recombinant plasmids were used to identify, by hybridization, corresponding wild-type dna sequences from an a. sesbaniae lambda embl3 genomic library (lambda asm phages). all 16 asm- vi mutants physically mapped to a single genomic locus. plasmid subclones of recombinant phag ...	1988	2830230
the major apoprotein of rabbit pulmonary surfactant. elucidation of primary sequence and cyclic amp and developmental regulation.	the major apoprotein of rabbit pulmonary surfactant is a developmentally and hormonally regulated sialoglycoprotein, mr congruent to 29,000-36,000 (sp 29-36). in the present study, specific antibodies were used to isolate cloned cdna inserts for sp 29-36 from a fetal rabbit lung cdna library in bacteriophage lambda gt11. two species of cdna of 1.9 and 3.0 kilobases (kb) in size were isolated that are complementary to two species of mrna of 2.0 and 3.0 kb which differ primarily in the lengths of ...	1988	2830270
initiation protein induced helix destabilization at the lambda origin: a prepriming step in dna replication.	the interaction of the lambda phage initiator protein, o, with the lambda origin sequence, ori, has been investigated. binding of o, or its amino-terminal fragment, causes a major structural change within a 60 bp at-rich region just to the right of the o-binding site. atp or other molecular energy sources are not required. the modification, as assayed by nuclease sensitivity, is reduced when certain ori mutant sequences, which bind o but fail to replicate, are substituted for the wild-type seque ...	1988	2830983
bending of the bacteriophage lambda attachment site by escherichia coli integration host factor.	escherichia coli integration host factor (ihf) is a small basic protein that is required for efficient integrative recombination of bacteriophage lambda. ihf binds specifically to sequences within attp, the site in bacteriophage lambda that undergoes recombination. it has been suggested that the binding of ihf creates bends in dna so as to help attp condense into a compact structure that is activated for recombination. in this work we show that ihf binding to either of two sites found within att ...	1988	2831189
the genetic structure of mouse ornithine transcarbamylase.	the gene encoding the mouse urea cycle enzyme, ornithine transcarbamylase has been isolated on five partially overlapping bacteriophage lambda clones. we have characterized the gene and found that it is split between ten exons distributed over approximately 70 kb of the x chromosome. the introns range in size from 88 bases to the relatively unusual size of approximately 26 kilobases, while the splice donor/splice acceptor sequences conform to the consensus established for other eukaryotic genes.	1988	2831503
genetic analysis of sequences in maltoporin that contribute to binding domains and pore structure.	maltoporin (lamb protein) is a maltodextrin transport protein in the outer membrane of escherichia coli with binding sites for bacteriophage lambda and maltosaccharides. binding of starch by bacteria was found to inhibit swarming of escherichia coli in soft agar plates; the inhibition was dependent on the maltodextrin affinity of maltoporin. on the basis of this observation, chemotactic cell-sorting techniques were developed for the isolation and analysis of mutants with an altered starch-bindin ...	1988	2832377