Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [dose/response relations of insulin and glucagon on honeybee glycemia]. | two hours after insulin-injection to emerging bees, hypoglucosemia occurs significantly with doses from 10(-2) to 10(2) ng per bee, with a maximum (-53 p. cent) from 0.1 to 1 ng, that is equivalent to 0.02 to 0.2 i.u./kg. hyperglucosemic effects of glucagon, 1 hour 30 mn after injection, are significant with doses from 10 to 500 ng per bee, with a maximum (+ 146 p. cent) for 100 ng, that is: 844 micrograms/kg. the trehalosemia shows lower variations in the same time. | 1980 | 6446353 |
| [inter-racial modifications of glycemia in the honeybee (apis mellifica l.)]. | the haemolymph levels of trehalose, glucose and fructose in foraging worker bees, have been compared in 4 different colonies of each of the following races: mellifica (m), ligustica (l), caucasica (c) and their hybrids. significant differences were found between races, with increasing values in the order: l leads to m leads to c leads to (l x c) x m; in the extreme cases, the amplitude of those differences reached a 1 to 4 range for glucose and 1 to 5 for fructose. the trehalose levels showed lo ... | 1980 | 6451277 |
| [treatment with bee products in acute and chronic pharyngo-laryngopathies]. | 2004 | 6454224 | |
| the first optic ganglion of the bee. v. structural and functional characterization of centrifugally arranged interneurones. | the organization, characterization and connectivity patterns of four different interneurone types were studied with the use of golgi light- and electron-microscopic techniques. all four cell types originate in the outer chiasma; they have an efferent end-branch in the lamina and an afferent one terminating in the distal region of the second optic ganglion, the medulla. these interneurones are referred to as: (i) garland-cell: the efferent fibre has on its tangential branch numerous centripetal s ... | 1984 | 6467336 |
| highly sensitive immunoassays based on use of liposomes without complement. | we describe a novel liposome-based immunoassay in which covalently linked hapten-cytolysin conjugates are used instead of complement and surface-immobilized immunoreagents. stable, unilamellar liposomes containing entrapped alkaline phosphatase as a marker enzyme were prepared by dialysis of octyl glucoside from suspensions of cholesterol and egg yolk lecithin. the resulting vesicles could be immediately lysed by addition of either bee venom melittin or hapten-melittin conjugates. using ouabain, ... | 1984 | 6467563 |
| the influence of melittin on the rotation of band 3 protein in the human erythrocyte membrane. | the rotational mobility of band 3, a protein constituent of the human erythrocyte membrane, was measured by observing the flash-induced transient dichroism of the triplet probe eosin maleimide. in the presence of melittin, a pharmacologically active polypeptide from honey bee (apis mellifera) venom, a dose-dependent loss of rotational mobility was detected. with acetylated melittin, the ability to immobilize is reduced. succinylated melittin, however, is devoid of immobilizing activity. the poss ... | 1984 | 6468341 |
| [acute renal failure associated with non-traumatic rhabdomyolysis caused by multiple bee stings]. | 1984 | 6471950 | |
| [severe complications caused by multiple bee stings]. | 1984 | 6471951 | |
| honeybee syndrome, glycolysis, and birth defects (continued) | 1984 | 6472392 | |
| meat tenderizer and bee stings. | 1984 | 6472988 | |
| bee venom allergy. | bee venom allergy is a common problem in medical practice. the immunological mechanisms and management thereof are reviewed. | 1984 | 6474293 |
| inhibitory effect of honey bee venom on immune complex mediated leukocyte migration into rabbit knee-joints. | the anti-inflammatory effect of purified honey bee venom (hbv) was studied using a recently described animal model in which preformed immune complexes were injected into rabbit knee-joints. as little as a single injection of 1.2 micrograms hbv/kg body weight subcutaneously significantly reduced the immune complex induced joint inflammation as measured by reduction in leukocyte counts in the joint fluid. this decrease was obvious 3 and 6 but not 9 hours after induction of the inflammation. there ... | 1984 | 6475661 |
| vitamin requirements of three spiroplasmas. | a chemically defined medium (cc-494m) was used to study the vitamin requirements of three spiroplasmas representing three distinct serogroups: flower spiroplasmas [spiroplasma floricola and fs (sr-3)] and honeybee spiroplasma [hbs (as-576)]. nicotinic acid and riboflavin were essential to spiroplasma growth. nicotinamide could substitute for nicotinic acid. populations of s. floricola, fs (sr-3), and hbs (as-576) reached 3.2 x 10(9), 1.96 x 10(10), and 6.1 x 10(9) cfu/ml, respectively, when nico ... | 1984 | 6480563 |
| [role of varroa jacobsoni oudemans in the bee family as a carrier of microorganisms]. | 1984 | 6485890 | |
| biological effects of a 765-kv, 60-hz transmission line on honey bees (apis mellifera l.): hemolymph as a possible stress indicator. | number of circulating hemocytes and hemolymph protein patterns of adult worker honey bees were analyzed as possible indicators of stress resulting from colony placement under a 765-kv transmission line. although exposure to 55, 80, and 95 microa total induced hive current (thc) produced colony behavioral disturbance, there were no consistent effects on mean hemocyte counts at 55- or 95-microa thc. age-dependent declines in circulating hemocyte number were similar in all exposure groups. there we ... | 1984 | 6487381 |
| skin and radioallergosorbent tests in patients with sensitivity to bee and wasp venom. | intradermal (id) and prick tests with bee or wasp venom (pharmalgen) have been performed on 102 subjects with a history of adverse reactions to stings and forty-six control subjects giving no such history. venom was diluted 100, 10 and 1 microgram/ml for prick testing and 10(-2), 10(-2), 10(-3) and 10(-4) micrograms/ml for id injections. in forty-six control subjects all were tested with the highest concentration of prick testing solution (100 micrograms/ml), eight (17%) had positive reactions, ... | 1984 | 6488511 |
| natural history of large local reactions from stinging insects. | in ongoing studies of the natural history of stinging-insect allergy, 133 patients with large local reactions have been evaluated over 8 yr; 79 patients returned for reevaluation. based on rast analysis with honeybee and vespid venoms, patients were divided into rast-positive and rast-negative groups. sixty-six patients were rast-negative with positive venom skin tests in 58%. seventy-five testings in this group led to no systemic reactions and 74 large local reactions. at follow-up rasts remain ... | 1984 | 6491095 |
| [when is desensitization with bee and wasp venoms indicated in children?]. | 1984 | 6493373 | |
| insect allergy. a comparative study including case histories and immunological parameters. | one hundred and seventeen persons all stung by yellow jacket (yj) and/or bee were examined by means of skin prick test with venom of these insects, skin prick test with 10 inhalant allergens and analyses of total ige, specific ige and igg against honey bee and yj venom. eighty-seven persons had had a systemic reaction to yj or bee sting, the rest had reacted normally or with a large local reaction. positive correlations (p less than 0.05) were found between results of skin prick tests and specif ... | 1984 | 6496884 |
| the characterization of high-affinity binding sites in rat brain for the mast cell-degranulating peptide from bee venom using the purified monoiodinated peptide. | the preparation of a pure, monoiodinated derivative of mast cell-degranulating peptide (mcd peptide), the mast cell-degranulating peptide from bee venom, has enabled us to identify binding sites in rat brain membranes that have a high affinity and specificity for this peptide. these binding sites are evenly distributed throughout the brain and copurify with synaptic membranes. saturation-binding curves, determined by rapid centrifugation or filtration assays, indicate a single population of site ... | 1984 | 6501283 |
| [effect of tryptophan and its metabolites on the conditioned reflex activity of the honeybee]. | the effect of tryptophane and its derivatives on the rate of elaboration and transformation of conditioned reflexes (cr) to odour, with alimentary reinforcement, was studied in wild bees under two conditions: free movement of the bee or its immobilization (stress situation), by means of genetic models (mutations, successive blocking stages of kynurenin path of tryptophane metabolism). it was shown that mutations eliciting accumulation of free tryptophane and serotonin in the hemolymph of the bee ... | 2016 | 6506871 |
| selective solubilization by melittin of glycophorin a and acetylcholinesterase from human erythrocyte ghosts. | melittin, the main basic and hydrophobic peptide of bee venom, has been used for solubilizing membrane components of the human erythrocyte ghost. up to 1.0 mm, it does not extract any phospholipid. between 0.1 and 1.0 mm, it solubilizes partially glycophorin a and acetylcholinesterase. when the membrane is first degraded by phospholipase a2, the solubilization of both proteins by melittin is total, and 48% of the phospholipids are removed, mainly as lysoproducts, whereas phospholipase a2, by its ... | 1984 | 6509055 |
| use of cell cultures to study spiroplasma infections. | invertebrate and mammalian cell cultures have been utilized to study various properties of spiroplasmas. recent studies have focused on the interaction of spiroplasma mirum with ag-4676, a cell line derived from the rat eye lens. s. mirum produced cytotoxicity in this cell line, consisting of vacuolization and an increase in the number of binucleated cells. the number of s. mirum organisms attached to the cell increased with increased age of infection. s. mirum does not grow in dulbecco's modifi ... | 1984 | 6511318 |
| eosinophilic cellulitis (wells' syndrome): histologic and clinical features in arthropod bite reactions. | five patients are described with the clinical and histopathologic picture, including flame figures, of eosinophilic cellulitis (wells' syndrome). two of them had documented tick bites in the center of these expanding annular lesions, and the histologic picture showed the diagnostic flame figures of wells' syndrome (eosinophilic cellulitis). a third patient had a clinical picture suggestive of a tick bite reaction but stated she was stung by a small garden bee at the involved site. the fourth pat ... | 1984 | 6512050 |
| bee venom and adjuvant induced disease. | 1984 | 6512799 | |
| the humoral immune response in the american cockroach, periplaneta americana: reactivity to a defined antigen from honeybee venom, phospholipase a2. | our previous experiments demonstrated that honeybee venom could induce a specific, adaptive humoral immune response in the american cockroach. since honeybee venom is a complex substance made up of several proteins, a more defined antigen is needed for future characterization studies. one of the components of bee venom, phospholipase a2 (pa2) was found to be highly lethal and immunogenic in the roach. roaches injected with pa2 generated a specific primary response that developed over a period of ... | 1984 | 6519337 |
| nephrotic syndrome following a bee sting. | 1984 | 6520578 | |
| [diagnostic and therapeutic aspects in the treatment of bee venom allergy]. | in 50 patients who had severe allergic general reactions after a bee sting clinical and immunological parameters were examined under the desensitisation with apitoxin. under the introduction of the hospital treatment 25 patients showed side effects. 14 of these patients had milder general reactions also during the further continuation of the ambulatory treatment. in no case the treatment had to be stopped. the behaviour of the specific ige and igg antibodies does not allow a prognostic evidence ... | 1984 | 6523947 |
| melittin and the 8-26 fragment. differences in ionophoric properties as measured by monolayer method. | melittin is a major (approximately 50%) protein component of bee venom. this peptide is an amphiphilic protein, because, while the amino acid residues 1-20 are predominantly hydrophobic (with the exception of lys-7), residues 21-26 are hydrophilic. the binding properties to vesicles and lipid bilayers of melittin have provided much useful information regarding biological (hemolytic) activity (habermann, e., 1972, science [wash. dc], 177:314-322). recent studies have convincingly established that ... | 1984 | 6547621 |
| nucleotide sequence of cloned cdnas coding for preprosecapin, a major product of queen-bee venom glands. | total mrna from queen-bee venom glands was transcribed into cdna and cloned into the psti and clai site of pbr322. the nucleotide sequence of two clones (pum9/24 and pbmc1) with inserts derived from mrnas for preprosecapin is presented. the two inserts, which are identical in their overlapping regions, have a coding region encompassing 77 triplets. the cloned cdnas differ in the length of the 3'-untranslated region, which is about twice as long in clone pum9/24. the postulated amino acid sequenc ... | 1984 | 6548708 |
| hyperreactivity to bee stings: reevaluation. | (a) a survey of beekeepers in israel did not show that they differed allergy-wise from the general population. (b) twenty-eight non-beekeeper systemic reactors to bee stings were not necessarily more allergic or more exposed to stings than the others. these systemic reactors had a spontaneous kinin system hyperreactivity and in some of them changes in the immune system and a complement defect were detected. we think that predisposition may enhance the likelihood of a systemic reaction to bee sti ... | 1983 | 6552888 |
| [therapeutic use of bee royal jelly]. | 1983 | 6555102 | |
| characterization of the purified anticoagulant principles from apis mellifera (honey bee) venom. | 1983 | 6579218 | |
| development of encephalopathic features similar to reye syndrome in rabbits. | the progression of neurological abnormalities through four or five clinically distinguishable levels of deepening coma and the development of a fatty liver are the hallmarks of reye syndrome. a number of animal models have been described that result in fatty liver formation with minimal, static, or catastrophic neurological changes. in this study, we attempted to produce neurological features in rabbits that reflected a rostral-caudal progression of abnormalities that could be categorized into c ... | 1984 | 6592608 |
| serologic aspects of igg4 antibodies. i. prolonged immunization results in an igg4-restricted response. | labeled antigen-binding tests were used to determine quantitatively the contribution of igg4 antibodies to the total igg antibody response in humans. in agreement with literature, we found no igg4-restricted antibody responses with tetanus toxoid or streptococcal carbohydrate. in the serum of individuals immunized for several years with phospholipase (pla) from honey bee venom, grass pollen allergen, or house dust mite allergen, we often found that more than 50% of the total antigen-binding capa ... | 1983 | 6600252 |
| reduction of interference by specific igg with a modified microtiter solid-phase radioimmunoassay to measure honeybee venom ige. | by means of monoclonal mouse anti-human ige antibody, the microtiter solid-phase radioimmunoassay (mspria) was modified to measure honeybee venom (hbv) ige. hbv igg was measured by mspria(g) using affinity column purified goat anti-human igg antibody and correlated with results obtained with staphylococcal protein a-spria. nonlinear specific ige dilution curves were associated with high hbv igg content. by serial transfer and assay of the supernatant, the interference of ige by specific igg coul ... | 1983 | 6600761 |
| honey bee venom specific immunoglobulin g4 in honey bee sting allergic patients and bee keepers. | honey bee venom (hbv) igg4 antibody was studied in bee keepers and honey bee sting allergic patients on immunotherapy using mouse monoclonal anti-human igg4 antibody in a modified microtiter solid phase radioimmunoassay (mspira). the mean hbv igg4 in bee keepers was 31 units/ml. in patients the mean hbv igg4 pre- and post-immunotherapy were 2.9 and 11.6 units/ml, respectively, representing a mean rise of 7.3-fold as compared to a rise of 1.6-fold for total hbv igg. the mean hbv igg level in bee ... | 1983 | 6600888 |
| antibodies to purified bee venom proteins and peptides. i. development of a highly specific rast for bee venom antigens and its application to bee sting allergy. | ige antibodies to purified proteins and peptides from honeybee venom have been measured by the rast. trace amounts (less than 0.1%) of the major venom protein phospholipase a2 (pla2) grossly distorted the measurement of ige antibody to the other venom proteins, acid phosphatase (acid p) and hyaluronidase (hyal), and overemphasized their importance. reduction of antigen coupled to the cellulose paper discs, which were used in the assay, diluted out the contaminating pla2 without apparent loss in ... | 1983 | 6601672 |
| venom-specific ige and igg antibodies as a measure of the degree of protection in insect-sting-sensitive patients. | venom-specific ige and igg antibodies were measured in the sera of bee-venom-sensitive patients during a 3-year hyposensitization period. the level of specific igg antibodies initially increased, and 2 months after the start of therapy, this increase was on average five-fold. a concomitant but non-significant increase in specific ige antibodies was also observed initially. later during the treatment period specific ige antibodies showed a continuous decline and after 3 years the level was one th ... | 1983 | 6602010 |
| antibody responses to honey-bee venom and monomethoxy-polyethylene glycol-modified honey-bee venom in mice. | antibody responses of the ige isotype were raised in mice with honey-bee venom (hbv) administered in alum or by daily injections without adjuvant. the sensitized mice were treated with single injections of hbv modified with monomethoxy-polyethylene glycol. by such treatment with modified but not with natural hbv, suppression of the ige antibody responses was achieved. the igg antibody responses, in contrast, were unchanged or enhanced. | 1983 | 6602097 |
| [field trials to control the bee tick varroa jacobsoni with folbex-va-neu and biotechnical measures on a bee farm in tunisia]. | 1983 | 6615415 | |
| the coelocapitular sensillum, an antennal hygro- and thermoreceptive sensillum of the honey bee, apis mellifera l. | the sensillum coelocapitulum, a hygro- and thermoreceptive sensillum of the honey bee, apis mellifera, was investigated by electron microscopy. the cuticular apparatus of the sensillum is a mushroom-shaped protrusion, devoid of pores, set in a narrow cylindrical pit positioned centrally within a cuticular, shallow depression. there may be three or four receptor cells. three receptor cells have unbranched sensory cilia, containing densely packed microtubules, which extend distally into the cuticu ... | 1983 | 6616572 |
| antibodies to purified bee venom proteins and peptides. ii. a detailed study of changes in ige and igg antibodies to individual bee venom antigens. | antibodies to individual bee venom antigens were studied in detail in nine bee sting-allergic patients who received venom immunotherapy without side effects, in two patients who failed to reach maintenance, and in two whose sensitivity returned. the study was confined to patients who had ige antibodies to at least one of four purified bee venom antigens at the start of treatment. ige and igg antibodies to phospholipase a2 (pla2), hyaluronidase (hyal), and acid phosphatase (acid p) and ige antibo ... | 1983 | 6619452 |
| allergens in hymenoptera venoms. x. vespid venoms versus venom sac extracts: comparison by two-dimensional polyacrylamide gel electrophoresis. | vespid venoms were compared to venom sac extracts by two-dimensional polyacrylamide gel electrophoresis using non-equilibrium ph gradient electrophoresis in the first dimension and sodium dodecyl sulfate electrophoresis in the second. the gels were stained with silver. fresh venoms from four species, vespula maculifrons, polistes fuscatus fuscatus, p. metricus and p. exclamans, were compared with commercially available venom sac extracts from the same species. in each case the venom sac extract ... | 1983 | 6625227 |
| environmental research on potential pesticides: indispensability and scope under conditions of poland. | in consideration of the ubiquitous presence of pesticides in the environment, research should be done for each new compound aimed at evaluation of the hazard resulting from its application. environmental investigations conducted in poland encompass research on: (a) abiotic transformations (hydrolysis, photodegradation), (b) metabolism in plants and animals, as well as the fate in aquatic and soil environments; (c) influence on biota (including microflora, honey-bee and other beneficial arthropod ... | 1983 | 6630895 |
| venom immunotherapy in hymenoptera sting allergy. comparison of rush and conventional hyposensitization and observations during long-term treatment. | 42 patients with confirmed hypersensitivity to honey bee (hbv) and/or yellow jacket (yjv) were treated with the respective venoms (7 with hbv, 5 with vjv and 30 with both venoms). treatment tolerance, skin tests (st), specific ige- and specific igg-antibodies were monitored before, after 3, 6, 12, 24 and 36 months. 21 patients had a rush and 21 a conventional treatment schedule. maintenance dose was 100 micrograms. adverse effects occurred as large local (8 patients), slight systemic (12 patient ... | 1983 | 6638413 |
| [desensitisation with hymenoptera venom in children]. | the efficiency of immunotherapy with hymenoptera venom is well known in adults. its clinical tolerance and its immunologic consequences during an accelerated desensitization program were studied in children with bee venom (n = 12, average age: 9.4 +/- 4.6 years) or wasp venom (n = 6, average age: 10.4 +/- 3.7 years) hypersensitivity. results were compared with those obtained in adults susceptible to bee venom (n = 15, average age: 36.6 +/- 10.4 years) or to wasp venom (n = 18, average age: 36.2 ... | 2006 | 6639281 |
| facilitation of phospholipase a2 activity by mastoparans, a new class of mast cell degranulating peptides from wasp venom. | the effect of mastoparan, ile-asn-leu-lys-ala-leu-ala-ala-leu-ala-lys-lys-ile-leunh2, and related peptides on the release of arachidonic acid from egg yolk lecithin liposomes, rat peritoneal mast cells, and cultured human fibroblasts was studied. in unsonicated liposomes, labeled with 1-stearoyl-2[1-14c]arachidonyl-sn-glycero-3-phosphocholine, 5 x 10(-5) m mastoparan caused a 12-, 15-, and 50-fold increase in the production of arachidonic acid catalyzed by phospholipase a2 from bee venom, easter ... | 1983 | 6643447 |
| the relative value of skin tests and radioallergosorbent test in the diagnosis of bee sting hypersensitivity. | the relative diagnostic value of st and rast was evaluated in 97 patients with bsh. eighteen patients had lrs, 79 showed srs including 18 with urticaria, 26 had bronchospasm, and 35 had anaphylactic shock. st but not the rast reactivity was strongly related to the severity of the clinical reaction (p less than or equal to 0.001) and found superior to the rast in identifying patients with srs in whom venom therapy was indicated. | 1983 | 6643873 |
| population structure and mdh-1 locus variation in apis mellifera ligustica. | in a wide area of the piedmont of italy the apiaries of apis mellifera ligustica spin., (the italian bee) show homogeneous allelic frequency distributions at the mdh-1 locus, the only one known to be polymorphic in worker bees. this can be explained by considering that an apiary is not a closed genetic system and that among apiaries gene flow is sufficient to overcome the different forces of inbreeding and random genetic drift. nevertheless there is some evidence for partial subdivision because ... | 1983 | 6643985 |
| microspectrophotometry of single rhabdoms in the retina of the honeybee drone (apis mellifera male). | the relative absorption spectra of the bistable photopigment of single rhabdoms from the dorsal region of the retina of the honeybee drone were obtained using slices of retina fixed in glutaraldehyde; less accurate measurements on unfixed tissue gave difference spectra that were similar to those for fixed retinae. the method used was based on measurements of absorbance changes during saturating adaptations of the visual pigment to different monochromatic lights. it is similar to previous methods ... | 1983 | 6644268 |
| changes in the levels of anti-phospholipase a2 and hyaluronidase antibodies during bee venom immunotherapy. | 1983 | 6646134 | |
| [diffuse immunoblastic reaction following a bee sting (report of a case)]. | 1983 | 6650789 | |
| structural changes in melittin and calmodulin upon complex formation and their modulation by calcium. | in the presence of ca2+, calmodulin forms a 1:1 high-affinity complex (kd = 3 nm) with melittin, a peptide from bee venom; in the presence of ethylenediaminetetraacetic acid, a second type of complex, of much lower affinity, is formed [comte, m., maulet, y., & cox, j. a. (1983) biochem. j. 209, 269-272]. in this paper, these interactions were studied by tryptophan fluorescence and circular dichroism spectroscopy in near- and far-uv. interaction between the two peptides in the presence as well as ... | 1983 | 6652077 |
| contact dermatitis due to honeybee royal jelly. | a woman who had ingested honeybee royal jelly as a nutrient, showed an exacerbation of dermatitis when it was applied to her feet. a topical fungicide also aggravated her skin lesions. patch testing showed positive reactions to the royal jelly, pyrrolnitrin in the fungicide and urushiol. positive reactions to the royal jelly were found in 2 out of 10 controls, 1 of whom was sensitive to propolis. | 1983 | 6653102 |
| composition and protein quality of honeybee-collected pollen of eucalyptus marginata and eucalyptus calophylla. | the composition and protein quality of the two most important western australian export-quality pollens were investigated. crude pollen protein content was 20.6% and 27.9% for jarrah (eucalyptus marginata) and marri (eucalyptus calophylla), respectively. lysine was the limiting amino acid relative to the fao protein scoring pattern (food and agriculture organization of the united nations), and the amino acid scores were 0.73 and 0.66 for jarrah and marri pollen, respectively. apparent biological ... | 1983 | 6655512 |
| effects of extracellular calcium and of light adaptation on the response to dim light in honey bee drone photoreceptors. | light responses in honey bee drone photoreceptors were recorded with intracellular micro-electrodes in superfused slices of retina. the effects of changes in extracellular calcium on the size and the shape of the response to dim light were studied and compared with the effects of light adaptation. dim light stimuli were used so that the amplitude of the response was linearly related to the number of the photons absorbed, the effects of voltage-dependent mechanisms were negligible and no detectab ... | 1983 | 6655592 |
| [theoretical conformation analysis of mcd peptide]. | the spatial structure of the mcd-peptide from bee venom has been calculated basing on the known sequence of 22 amino acid. the a priori calculations produce a system of two disulfide bonds, identical to that observed in the native structure. the calculated structure of mcd-peptide is close to that proposed earlier for the homologues peptide tertiapin and is confirmed by nmr and cd data. | 2006 | 6656752 |
| neurobiology of learning and memory: the honeybee as a model system. | 1983 | 6656885 | |
| the stinging response of the honeybee: effects of morphine, naloxone and some opioid peptides. | changes in responsiveness for the stinging reaction of honeybees fixed in a holder after receiving 3 electrical shocks delivered with 1 min interval, was registered and used as measurement for the effect of 2 microliter of different solutions injected. every shock consisted of a train of pulses of 1 msec each, delivered for 2 sec at a frequency of 100 hz. injection of morphine-hcl (50 to 200 n-moles/bee) produced a dose dependent reduction of the honeybee stinging response to the electrical shoc ... | 1983 | 6657718 |
| [fatal envenomation by multiple bee stings (apis mellifera l.)]. | 1983 | 6658267 | |
| the glycoprotein nature of phospholipase a2, hyaluronidase and acid phosphatase from honey-bee venom. | experiments with immobilized concanavalin a strongly suggest a glycoprotein nature of three honey-bee venom enzymes, phospholipase a2, hyaluronidase and acid phosphatase. the electrophoretically and chromatographically detectable heterogeneity of phospholipase a2 results from absence of carbohydrate in a subfraction. mannose, fucose and n-acetylglucosamine, but not galactose nor n-acetylgalactosamine, are present in the con a-binding fraction of bee venom. it is therefore concluded that only n-g ... | 1983 | 6658811 |
| esterase isozymes of apis mellifera: substrate and inhibition characteristics, developmental ontogeny, and electrophoretic variability. | starch gel electrophoresis utilizing different types of substrates and inhibitors made it possible to detect several esterases in crude extracts of apis mellifera. our results suggest that there are six apis mellifera esterase isozymes (esterases 1-6) that differ not only in electrophoretic mobility but also in substrate specificity and inhibition properties. some of the esterase isozymes are controlled by more than one allele. the frequency of these genetic variants was analyzed in four populat ... | 1983 | 6661179 |
| [release of 14c-acetylcholine from synaptosomes as affected by presynaptic neurotoxins from bee and cobra venoms--phospholipases a2]. | the effect of presynaptic neurotoxin from bee and cobra venom--phospholipases a2 on na+-dependent high affinity [14c]choline transport from the striate body of rat brain into synaptosomes has been studied. it was shown that both phospholipases a2 inhibit the re-uptake of [14c]choline and specifically stimulate the release of [14c]acetylcholine from the synaptosomes. this effect is especially well-pronounced for bee venom phospholipase a2. it was assumed that damages of biochemical processes on t ... | 1983 | 6661452 |
| [measurement of bee venom specific igg antibody in bee venom hypersensitivity and the relation between the specific ige antibody and total ige]. | bee venom specific igg antibodies for honeybee and common wasp were measured in normal control group, slightly bee injury group, heavy bee injury group and honeybee keeper's group. relation among bee venom specific igg antibody, bee venom specific ige antibody and total ige was analyzed. normal control level of bee venom specific igg antibody was measured under 60 u/ml. bee venom specific igg antibody in slightly bee injury group was higher than that in heavy bee injury group. bee venom specific ... | 1983 | 6665232 |
| changes induced by apamin from bee venom on differentiated mouse neuroblastoma cells in culture. | the minor active component of bee venom was applied to mouse neuroblastoma cultures. the cytological changes observed are reported. after 8-10 h of incubation with 5 micrograms/ml of apamin in the culture medium, considerable retractions of the processes are apparent. electron microscopically, the alterations seen are predominantly found in the subcellular organelles. the peculiar configuration of the endoplasmic reticulum (er) is striking. concentric whorls of cisternae seem to engulf the remai ... | 1983 | 6666537 |
| bee venom immunotherapy. | 2004 | 6669119 | |
| [bee mite: varroa jacobsoni qudemans]. | varroatosis caused by varroa jacobsoni on honeybee, apis mellifera l., is currently one of the worlds major bee keeping problems. the mite parasites the adult honey bee, as well as its developmental stages, by sucking the insects's haemolymph. up to date, many chemicals were used against this mite but still there is no chemical which has 100% effect and at the same time bees and their brood demonstrate a good tolerance. the investigations on biology and therapy on varroa are still going on in ma ... | 1983 | 6672554 |
| structural aspects of the interaction of bee venom peptide melittin with phospholipids. | 1983 | 6673766 | |
| age and oxygen toxicity related fluorescence in the honey bee thorax. | the blue fluorescent material in thoraxes of honey bees of known adult age from eclosion to 28 days was measured. the amount was greater in older insects and exposure to ozone (ppm) augmented the rate of accumulation. the nearly constant daylight flying by the bees and the ozone effect suggest radical peroxidative origin of the fluorescence. the material was partially purified by chromatography. it is a relatively non-polar lipid. | 1983 | 6673991 |
| vitamin e and stabilization of membrane lipid organization in red blood cells with peroxidative damage. | the phospholipids distribution across red cell membrane bilayer is asymmetrical. phosphatidylcholine (pc) and sphingomyelin (sm) are predominantly present in the outer, and phosphatidylserine (ps) and phosphatidylethanolamine (pe) are predominantly present on the cytoplasmic side of the red cell membrane. the present study reports the effect of fatty acid peroxidation on the organization of ps and pe in human red cells using a nonpermeable bee venom phospholipase-a2 which specifically hydrolyzes ... | 1983 | 6675717 |
| [igg4 antibody response to phospholipase a in bee keepers]. | 1983 | 6677246 | |
| substrate utilization in defined media. | substrate utilization in defined media for two flower spiroplasmas (s. floricola and fs sr-3) and honeybee spiroplasma (hbs as-576) was investigated. glucose, fructose, and mannose were utilized by all three spiroplasmas. in addition, hbs (as-576) could ferment trehalose; fs (sr-3), sucrose; and s. floricola, trehalose, sucrose, and raffinose. the three spiroplasmas varied greatly in growth requirements for amino acids. only s. floricola utilized arginine. hbs (as-576) required at least one puri ... | 1983 | 6679142 |
| the honeybee syndrome: teratogenic effects of mannose during organogenesis in rat embryo culture. | the relevance of our present findings should not rest on the possible role of mannose as an important teratogen in man. excessive exposure to mannose during pregnancy via dietary intake seems unlikely since mannose is absorbed poorly from the gastrointestinal tract and intestinal hydrolysis of mannosidic linkages may be minimal. moreover, although some plasma mannose may be generated continuously from endogenous sources via the cleavage of mannose-6-phosphate by hepatic glucose-6-phosphatase or ... | 1983 | 6679963 |
| lipases production by bacillus circulans under mesophilic and osmophilic conditions. factors affecting lipases production. | a facultative osmophilic bacterium able to produce lipases (isolated from bee honey) was identified as bacillus circulans. factors affecting lipases production by such isolate were studied. dox's liquid medium supplied with 2 and/or 30% sucrose was used as a basal medium, under static culture condition, for such a purpose. results have showed that the optimal sucrose concentration at which the isolate produced its maximal yield of lipases was 30% after an incubation period of 24 hours at 40 degr ... | 1983 | 6681018 |
| cooperative effects in the interaction between melittin and phosphatidylcholine model membranes. studies by temperature scanning densitometry. | the interaction between the peptide melittin from bee venom with multilamellar liposomes of dipalmitoylphosphatidylcholine or egg yolk phosphatidylcholine has been studied by the method of temperature scanning densitometry, yielding information on the specific volume of the association products and on the changes during the thermotropic transition of the lipids. the effects of the interaction were found to be biphasic with respect to melittin concentration; below 10(-3) mol per mol of phospholip ... | 1983 | 6687386 |
| phospholipids chiral at phosphorus. preparation and spectral properties of chiral thiophospholipids. | the thiophospholipid 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (dppsc) was shown to be a mixture of two diastereomers by 31p nuclear magnetic resonance. the isomer that resonates at the lower field in cdcl3 (56.12 ppm) was designated as isomer a and the other (resonates at 56.07 ppm) as isomer b. phospholipase a2 from four different sources (bee venom, naja naja venom, crotalus adamanteus venom, and porcine pancreas) was shown to hydrolyze the isomer b of dppsc specifically, whereas phosph ... | 1983 | 6688028 |
| the honeybee syndrome - implications of the teratogenicity of mannose in rat-embryo culture. | lethal effects of d-mannose in the honeybee have been recognized for more than a half a century. we observed another toxic effect of d- mannose during culture of rat embryos from the early head-fold stage to the 26-to-29-somite stage (days 9-1/2 through 11-1 of gestation). the addition to culture mediums of 1.5 mg of d-mannose per milliliter caused growth retardation and faulty neural-tube closure in approximately two thirds of the embryos. mannose effects occurred during the first 24 hours of c ... | 1984 | 6690938 |
| use of dialyzed venoms in the radioallergosorbent test. | optimal concentrations of honeybee, hornet and yellow jacket venoms were used in the radioallergosorbent test (rast) before and after dialysis. rast titers in the majority of sera from insect-allergic patients increased significantly with the dialyzed yellow jacket and hornet venom-coupled discs. of 26 sera which had negative rasts with crude venom, 20 became positive with dialyzed venom. no change in rast results was found with crude and dialyzed honeybee venom. the low molecular weight compone ... | 1984 | 6693178 |
| bee-sting mortality in australia. | death as a result of a bee sting is uncommon in australia. during the 22 years from 1960 to 1981, 25 individuals have been recorded by the australian bureau of statistics as having died shortly after a bee sting. this gives a mortality incidence of 0.086/1 000 000 population per year, but may be an underestimate, as we report two additional fatalities that did not appear in the records of the bureau of statistics. south australia has the highest mortality rate from bee stings of all the australi ... | 1984 | 6694624 |
| bee venom immunotherapy. | 1984 | 6694635 | |
| the purification and characterisation of hyaluronidase from the venom of the honey bee, apis mellifera. | hyaluronidase has been purified from the venom of the honey bee, apis mellifera. the purification proved remarkably difficult, requiring a large number of chromatographic steps culminating in the removal of traces of phospholipase a2 with an affinity purified rabbit anti-phospholipase a2 immunosorbent column. the purified enzyme showed a 1143-fold increase in specific activity and was homogeneous. electrophoresis in polyacrylamide gels (12%) containing sodium dodecyl sulphate (ph 8.9) or urea (p ... | 1984 | 6698011 |
| studies of coexisting honeybee and vespid-venom sensitivity. | honeybee and vespid venom-specific ige were measured by rast in randomly selected sera of 87 patients who had had anaphylactic reactions after insect stings. overall there was a poor correlation between the titers of honeybee venom and yellow jacket or hornet venom-specific ige. sera from nine patients with high titers of both honeybee venom and yellow jacket venom-specific ige were selected for rast-inhibition studies, with these two venoms as coupling and inhibiting antigens. three patterns of ... | 1984 | 6699307 |
| clinical and immunologic survey in beekeepers in relation to their sensitization. | beekeepers often present allergic symptoms and represent a natural and experimental model of anaphylaxis and specific immunotherapy. two hundred bks were investigated. both allergic and nonallergic subjects were selected very carefully in terms of immunologic status and exposure to bee stings. they were surveyed by a questionnaire, the titration of total serum ige, and bee venom-specific ige and igg. skin tests to hbv were performed in 176 subjects. skin test sensitivity to hbv and allergic symp ... | 1984 | 6699314 |
| [varroatosis. a zootechnical system of control]. | a managerial method is described by which the number of varroa mites in a bee colony may be reduced to a level not interfering with economic bee-keeping. this theoretical approach is based on the removal of combs in the month of may, when about ninety per cent of the mites have enclosed themselves in cells with developing larvae and puppae, to multiply. | 1984 | 6701873 |
| the overlearning-extinction effect and successive negative contrast in honeybees (apis mellifera). | in three experiments with free-flying honeybees, the previously discovered overlearning-extinction effect was replicated under different conditions and shown to depend on magnitude of reinforcement: the effect appeared in training with a 50% sucrose solution but not with a 20% solution. the results prompted a fourth experiment in which successive negative contrast was demonstrated: the animals were disturbed to find the 20% solution on a distinctive target that always before had been baited with ... | 1984 | 6705502 |
| polyethylene glycol reactive antibodies in man: titer distribution in allergic patients treated with monomethoxy polyethylene glycol modified allergens or placebo, and in healthy blood donors. | antibodies to polyethylene glycol (peg) were analyzed in patients with various allergies and in healthy blood donors employing passive hemagglutination. in untreated allergic patients and in healthy blood donors, naturally occurring anti-peg antibody titers between 32 and 512 were seen in 3.3 and 0.2%, respectively. during hyposensitization with monomethoxy polyethylene glycol modified ragweed extract and honey bee venom, respectively, the patients showed an anti-peg antibody response. titers of ... | 1984 | 6706424 |
| the accumulation of malonyldialdehyde, a product of fatty acid peroxidation, can disturb aminophospholipid organization in the membrane bilayer of human erythrocytes. | the distribution of phospholipids across erythrocyte membrane bilayer is asymmetrical. the present study reports the effect of malonyldialdehyde (mda), a product of fatty acid peroxidation, on the organization of phosphatidylserine (ps) and phosphatidylethanolamine (pe) in human erythrocytes using a nonpermeable bee venom phospholipase a2 and trinitrobenzene-sulfonilic acid. mda accumulation in the erythrocytes was accomplished both by its increased endogenous generation after exposure of cells ... | 1984 | 6706963 |
| bee venom immunotherapy and bee-sting mortality in australia. | 1984 | 6708906 | |
| allergens in hymenoptera venom xii: how much protein is in a sting? | the protein content of insect venoms was determined using a coomassie blue dye binding assay as well as the lowry phenol assay. the lowry method tended to give excessively high values with many of the venoms because of the presence of low molecular weight phenolic compounds and other interfering substances. using the dye binding method honey bee stings were found to contain 59 +/- 7 micrograms of protein, bumblebee and carpenter bee stings from 10 to 31 micrograms, yellow jacket stings from 1.7 ... | 1984 | 6711914 |
| pathogenicity of mollicutes for insects: possible use in biological control. | acholeplasmas, spiroplasmas and other non-helical sterol-requiring mycoplasmas of unknown phylogenetic affinity inhabit insects. of these, only spiroplasmas are known to be pathogenic. group i-2 spiroplasmas, or spiroplasma apis, especially in combination with other organisms, reduce honey-bee longevity. plant pathogenic mycoplasma-like organisms are often found intracellularly in insects. spiroplasmas are found predominantly in the gut lumen or haemolymph (or both) of their insect hosts. pathog ... | 1984 | 6712057 |
| studies on the pathogenicity of spiroplasmas for drosophila pseudoobscura. | representatives of several currently available spiroplasma serovars were used in feeding and injection experiments involving drosophila pseudoobscura adult flies in order to evaluate the host-range of the spiroplasmas and to determine their pathogenicity for flies. many of the isolates injected into flies either do not survive or grow poorly, and have no negative effects on longevity or fecundity. two spiroplasma strains, honey-bee (serovar i-2) and cornstunt (serovar i-3), can grow to high titr ... | 1984 | 6712059 |
| nutrition and the allergic athlete. | nutritional management of the allergic athlete centers around providing a diet adequate to meet the increased needs of the athlete at the same time that it is modified by the exclusion of any problematic foods. the athlete has an increased need for water, total energy, carbohydrate, b vitamins, and perhaps protein, the last two of which are usually met when the diet fulfills the energy requirements of the athlete. requirements for electrolytes are minimally increased, and the need for additional ... | 1984 | 6715737 |
| honeybee syndrome, glycolysis, and birth defects. | 1984 | 6717550 | |
| erythrocyte membrane glycerophospholipid organization is normal in multiple sclerosis. | the phospholipid composition of erythrocyte membranes from patients with multiple sclerosis (ms) was found to be normal, in agreement with previous reports. the transbilayer asymmetry of the glycerophospholipids in ms red cells was probed using bee venom phospholipase a2 and was also found not to be significantly different from normal. abnormal membrane glycerophospholipid organisation is therefore not involved in the increased red cell size, osmotic fragility, and electrophoretic mobility assoc ... | 1984 | 6717729 |
| blepharochalasis after a bee sting. | blepharochalasis is a distinct entity of redundant eyelids. it is characterized by a history of recurrent edema that leads to permanent changes in the eyelids. even in the young, blepharoplasty may be indicated for psychological reasons or to relieve obstruction of vision, eyelid asthenia, and fatigue. following surgery, recurrent edema may compromise the results or cause further eyelid changes. effort should be made to define the etiology of the edema and correct the defect if possible. | 1984 | 6718584 |
| bee sting of the cornea. | irreversible heterochromia-iridis, internal ophthalmoplegia, and punctate subcapsular lenticular opacities developed in a 9-year-old girl after she received a bee sting in her right cornea. these complications persisted even after an 11-month follow-up period. to the author's knowledge, this presentation is the first of its nature. the pathogenesis of these changes is discussed and the literature is reviewed. | 1984 | 6721343 |
| phospholipid asymmetry in the membranes of intact human erythrocytes and in spectrin-free microvesicles derived from them. | phospholipase a2 from bee venom and naja naja has been used to study the orientation of phospholipids present in the membrane of intact human erythrocytes and in spectrin-free microvesicles derived from the cells by treatment with ca2+ and a23187. little difference between the cells and microvesicles was observed in the apparent accessibility of phospholipids to the enzyme, suggesting that the original lipid asymmetry was maintained in the absence of spectrin. however, incubation of the microves ... | 1984 | 6722143 |
| bee-sting anaphylaxis: the use of medical antishock trousers. | there is a large population of unprotected individuals who are susceptible to hymenoptera-induced anaphylaxis. medical antishock trousers (the mast suit) can play an important role in field treatment of anaphylactic shock. upon application, both blood pressure and cerebral perfusion are preserved despite clinically profound vasodilation. the mast suit mechanically increases peripheral resistance, analogous to the vasoconstrictive effects of adrenaline. in the two cases presented, the mast suit r ... | 1984 | 6731963 |
| allergens in hymenoptera venom xi. isolation of protein allergens from vespula maculifrons (yellow jacket) venom. | pure vespula maculifrons venom was demonstrated to contain five major allergenic proteins, which were all isolated from commercial venom sac extract. the five proteins: vmacl, mw 97,000; hyaluronidase, mw 46,000; vmac3, mw 39,000; phospholipase a and b, mw 34,000; and antigen 5, mw 22,000 were all demonstrated to be biochemically and immunologically distinct. all five proteins had significant allergenic activity, with phospholipase and hyaluronidase demonstrating the most ige binding with 39 ser ... | 1984 | 6736487 |