Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
---|
functional characterization of a soluble nadph-cytochrome p450 reductase from fusarium graminearum. | fusarium head blight is a devastating disease in wheat caused by some fungal pathogens of the fusarium genus mainly f. graminearum, due to accumulation of toxic trichothecenes. most of the trichothecene biosynthetic pathway has been mapped, although some proteins of the pathway remain uncharacterized, including an nadph-cytochrome p450 reductase. we subcloned a f. graminearum cytochrome p450 reductase that might be involved in the trichothecene biosynthesis. it was expressed heterologously in e. ... | 2017 | 28690182 |
plant lignans inhibit growth trichothecene biosynthesis in fusarium graminearum. | 2017 | 28699242 | |
variability of composition and effects of essential oils from rhanterium adpressum coss. & durieu against mycotoxinogenic fusarium strains. | the antifungal potency of the essential oils of rhanterium adpressum was evaluated against four mycotoxigenic strains of the genus fusarium. the essential oils were obtained, separately, by hydro-distillation of the aerial parts of r. adpressum (leaves and flowers). the parts were collected during the period of bloom (3 months) for 3 years. the gc-ms analysis revealed thirty-six compounds for the essential oils, divided into four classes of chemical compounds, with variable percentages according ... | 2017 | 28707037 |
chrysogine biosynthesis is mediated by a two-module nonribosomal peptide synthetase. | production of chrysogine has been reported from several fungal genera including penicillium, aspergillus, and fusarium. anthranilic acid and pyruvic acid, which are expected precursors of chrysogine, enhance production of this compound. a possible route for the biosynthesis using these substrates is via a nonribosomal peptide synthetase (nrps). through comparative analysis of the nrpss from genome-sequenced producers of chrysogine we identified a candidate nrps cluster comprising five additional ... | 2017 | 28708398 |
recombinant lipase from gibberella zeae exhibits broad substrate specificity: a comparative study on emulsified and monomolecular substrate. | using the classical emulsified system and the monomolecular film technique, the substrate specificity of recombinant gibberella zeae lipase (rgzel) that originates from gibberella zeae was characterized in detail. under the emulsified reaction system, both phospholipase and glycolipid hydrolytic activities were observed, except for the predominant lipase activity. the optimum conditions for different activity exhibition were also determined. compared with its lipase activity, a little higher rat ... | 2017 | 28718792 |
the binding mechanism between azoles and fgcyp51b, sterol 14α-demethylase of fusarium graminearum. | fusarium graminearum is the main pathogen of fusarium head blight (fhb), a worldwide plant disease and one of the major wheat diseases in china. the control of the fhb is mainly dependent on the application of dmis fungicides. fungal sterol 14α-demethylase enzymes (cyp51) are the main target for dmis fungicides. in order to investigate the binding mechanism between azoles and cyp51b in f.graminearum, the molecular modeling study and biological evaluation were performed. | 2017 | 28719051 |
a transposon-directed epigenetic change in zmcct underlies quantitative resistance to gibberella stalk rot in maize. | a major resistance quantitative trait locus, qrfg1, significantly enhances maize resistance to gibberella stalk rot, a devastating disease caused by fusarium graminearum. however, the underlying molecular mechanism remains unknown. we adopted a map-based cloning approach to identify the resistance gene at qrfg1 and examined the dynamic epigenetic changes during qrfg1-mediated maize resistance to the disease. a cct domain-containing gene, zmcct, is the causal gene at the qrfg1 locus and a polymor ... | 2017 | 28722229 |
regulatory roles of introns in fungicide sensitivity of fusarium graminearum. | although the roles of introns have been much debated in eukaryotic organisms, none of them have been functionally characterized in fusarium graminearum. in this study, we characterized the roles of introns in regulation of fungicide-sensitivity of f. graminearum. β2 tub, cyp51a and myosin-5 are important target genes of benzimidazoles, triazoles and cyanoacrylates, respectively. to explore the sensitivity regulation functions of introns in target genes, several detailed deletion studies were com ... | 2017 | 28730650 |
bacillomycin d produced by bacillus amyloliquefaciens is involved in the antagonistic interaction with the plant pathogenic fungus fusarium graminearum. | fusarium graminearum (teleomorph: ascomycota, hypocreales, gibberella, gibberella zeae) is a destructive fungal pathogen that threatens the production and quality of wheat and barley worldwide. controlling this toxin-producing pathogen is a significant challenge. in the present study, the commercially available strain bacillus amyloliquefaciens (bacteria, firmicutes, bacillales, bacillus) fzb42 showed strong activity against f. graminearum the lipopeptide, bacillomycin d, produced by fzb42, was ... | 2017 | 28733288 |
genome-wide identification and analysis of the mads-box gene family in bread wheat (triticum aestivum l.). | the mads-box genes encode transcription factors with key roles in plant growth and development. a comprehensive analysis of the mads-box gene family in bread wheat (triticum aestivum) has not yet been conducted, and our understanding of their roles in stress is rather limited. here, we report the identification and characterization of the mads-box gene family in wheat. a total of 180 mads-box genes classified as 32 mα, 5 mγ, 5 mδ, and 138 mikc types were identified. evolutionary analysis of the ... | 2017 | 28742823 |
nicotinamide mononucleotide and related metabolites induce disease resistance against fungal phytopathogens in arabidopsis and barley. | nicotinamide mononucleotide (nmn), a precursor of nicotinamide adenine dinucleotide (nad), is known to act as a functional molecule in animals, whereas its function in plants is largely unknown. in this study, we found that nmn accumulated in barley cultivars resistant to phytopathogenic fungal fusarium species. although nmn does not possess antifungal activity, pretreatment with nmn and related metabolites enhanced disease resistance to fusarium graminearum in arabidopsis leaves and flowers and ... | 2017 | 28743869 |
chemical composition and antifungal activity of the essential oil of zhumeria majdae, heracleum persicum and eucalyptus sp. against some important phytopathogenic fungi. | application of essential oils of medicinal plants is considered a safe and acceptable method for plant disease management to protect plants from pathogenic microorganisms. thus, in recent study, essential oils (eos) from zhumeria majdae, heracleum persicum (two iranian endemic plants) and eucalyptus sp. were assayed for their antifungal potential against ten phytopathogenic fungi, including fusarium graminearum, fusarium asiaticum, fusarium redolens f.sp. dianthus, fusarium verticillioides, fusa ... | 2017 | 28757068 |
in vitro evaluation of sub-lethal concentrations of plant-derived antifungal compounds on fusaria growth and mycotoxin production. | phytopathogenic fungi can lead to significant cereal yield losses, also producing mycotoxins dangerous for human and animal health. the fungal control based on the use of synthetic fungicides can be complemented by "green" methods for crop protection, based on the use of natural products. in this frame, the antifungal activities of bergamot and lemon essential oils and of five natural compounds recurrent in essential oils (citronellal, citral, cinnamaldehyde, cuminaldehyde and limonene) have bee ... | 2017 | 28758914 |
a review of conventional pcr assays for the detection of selected phytopathogens of wheat. | infection of phyllosphere (stems, leaves, husks, and grains) by pathogenic fungi reduces the wheat yield and grain quality. detection of the main wheat pathogenic fungi provides information about species composition and allows effective and targeted plant treatment. since conventional procedures for the detection of these organisms are unreliable and time consuming, diagnostic dna-based methods are required. nucleic acid amplification technologies are independent of the morphological and biochem ... | 2017 | 28772274 |
comparative analysis of the composition and active property evaluation of certain essential oils to assess their potential applications in active food packaging. | the antifungal, antibacterial, and antioxidant activity of four commercial essential oils (eos) (thyme, clove, rosemary, and tea tree) from romanian production were studied in order to assess them as bioactive compounds for active food packaging applications. the chemical composition of the oils was determined with the folin-ciocâlteu method and gas chromatography coupled with mass spectrometry and flame ionization detectors, and it was found that they respect the afnor/iso standard limits. the ... | 2017 | 28772407 |
c-coordinated o-carboxymethyl chitosan metal complexes: synthesis, characterization and antifungal efficacy. | a novel type of o-carboxymethyl chitosan schiff bases (o-cspx) was synthesized via a condensation reaction. after the coordination reaction of cupric ions, zinc ions and nickel ions, metal complexes (o-cspx-m) were achieved. the theoretical structure of o-cspx-m calculated by gaussian 09 reveals that the copper ions and nickel ions underwent dsp(2) hybridization, the zinc ions underwent sp(3) hybridization, and they all coordinated by the carbon atom in the p-π conjugate group. then, the structu ... | 2017 | 28774807 |
growth suppression of fusarium culmorum, fusarium poae and fusarium graminearum by 5-n-alk(en)ylresorcinols from wheat and rye bran. | alk(en)ylresorcinols (ar), a class of phenolic lipids, are regarded as antifungal compounds showing high potential for the use in plant protection, especially against fusarium head blight (fhb). in view of the very limited knowledge of the activity of single ar against fusarium species, the antifungal effect of crude extracts, fractions and isolated homologues from wheat and rye bran was determined. it was shown that the saturated ar are the active compounds in the extracts, whereas the presence ... | 2017 | 28784549 |
expression of hopai interferes with map kinase signalling in magnaporthe oryzae. | the pmk1 and mps1 map kinases are essential for appressorium formation and plant infection in magnaporthe oryzae. however, their exact roles during invasive growth are not clear because pmk1 and mps1 mutants are defective in penetration. to further characterize their functions after penetration, in this study we expressed the pseudomonas syringae effector hopai known to inactivate plant map kinases in m. oryzae. constitutive expression of hopai with the rp27 or trpc promoter resulted in defects ... | 2017 | 28799700 |
synergistic effect of different plant cell wall degrading enzymes is important for virulence of fusarium graminearum. | endo-polygalacturonases (pgs) and xylanases have been shown to play an important role during pathogenesis of some fungal pathogens of dicot plants, whilst their role in monocot pathogens is less defined. pg1 and xyr1 genes of the wheat pathogen fusarium graminearum encode the main pg and the major regulator of xylanase production, respectively. single and double disrupted mutants for these genes were obtained to assess their contribution to fungal infection. compared to wild-type strain, the ∆pg ... | 2017 | 28800710 |
the cereal pathogen fusarium pseudograminearum produces a new class of active cytokinins during infection. | the fungal pathogen fusarium pseudograminearum causes important diseases of wheat and barley. during a survey of secondary metabolites produced by this fungus, a novel class of cytokinins, herein termed fusarium cytokinins, was discovered. cytokinins are known for their growth promoting and anti-senescence activities and the production of a cytokinin mimic by what was once considered a necrotrophic pathogen that promotes cell death and senescence challenges the simple view that this pathogen inv ... | 2017 | 28802024 |
pka activity is essential for relieving the suppression of hyphal growth and appressorium formation by mosfl1 in magnaporthe oryzae. | in the rice blast fungus magnaporthe oryzae, the camp-pka pathway regulates surface recognition, appressorium turgor generation, and invasive growth. however, deletion of cpka failed to block appressorium formation and responses to exogenous camp. in this study, we generated and characterized the cpk2 and cpka cpk2 mutants and spontaneous suppressors of cpka cpk2 in m. oryzae. our results demonstrate that cpka and cpk2 have specific and overlapping functions, and pka activity is essential for ap ... | 2017 | 28806765 |
the selective antifungal activity of drosophila melanogaster metchnikowin reflects the species-dependent inhibition of succinate-coenzyme q reductase. | insect-derived antifungal peptides have a significant economic potential, particularly for the engineering of pathogen-resistant crops. however, the nonspecific antifungal activity of such peptides could result in detrimental effects against beneficial fungi, whose interactions with plants promote growth or increase resistance against biotic and abiotic stress. the antifungal peptide metchnikowin (mtk) from drosophila melanogaster acts selectively against pathogenic ascomycota, including fusariu ... | 2017 | 28811531 |
a-to-i rna editing is developmentally regulated and generally adaptive for sexual reproduction in neurospora crassa. | although fungi lack adenosine deaminase acting on rna (adar) enzymes, adenosine to inosine (a-to-i) rna editing was reported recently in fusarium graminearum during sexual reproduction. in this study, we profiled the a-to-i editing landscape and characterized its functional and adaptive properties in the model filamentous fungus neurospora crassa a total of 40,677 a-to-i editing sites were identified, and approximately half of them displayed stage-specific editing or editing levels at different ... | 2017 | 28847945 |
screening of lactic acid bacteria for anti-fusarium activity and optimization of incubation conditions. | anti-fusarium activities of lactic acid bacteria (lab) lactobacillus plantarum 299v, l. plantarum nrrl-4496, and lactobacillus rhamnosus vt1 were determined by a microdilution assay developed in this study against fusarium graminearum 08/rg/bf/51. a cell-free lactobacillus culture supernatant (cflcs) of l. rhamnosus vt1 had the highest anti-fusarium activity. response surface methodology was used to optimize the incubation conditions for production of cflcs. a box-behnken factorial design was us ... | 2017 | 28853625 |