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lipopolysaccharides of campylobacter jejuni serotype o:19: structures of o antigen chains from the serostrain and two bacterial isolates from patients with the guillain-barré syndrome.an o antigenic polysaccharide was liberated from the lipopolysaccharide of high m(r) from campylobacter jejuni serotype o:19 by acetic acid hydrolysis of the ketosidic linkage to lipid a. the structure of the polysaccharide was established in several one- and two-dimensional 1h and 13c nmr experiments, fast atom bombardment mass spectrometry and methylation linkage analysis of the permethylated glycan and its degradation products. it is concluded that the glycan is a derivative of hyaluronic aci ...19947506928
discrimination among thermophilic campylobacter species by polymerase chain reaction amplification of 23s rrna gene fragments.by comparing nucleic acid sequences determined for one of the most variable areas of 23s rrna genes of 23 campylobacter strains, we were able to identify regions specific for thermophilic campylobacter strains. oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase chain reaction. one primer pair selectively detected all thermophilic campylobacter species, while four other primer pairs allowed discrimination among the thermophilic species campyl ...19937508460
penner's serotype 4 of campylobacter jejuni has a lipopolysaccharide that bears a gm1 ganglioside epitope as well as one that bears a gd1 a epitope.the carbohydrate structures of lipopolysaccharides (lpss) of campylobacter jejuni strains belonging to penner's serotypes (pen) 1, 2, 4, 19, 23, and 36 were studied by thin-layer chromatography and immunostaining with several monoclonal antiganglioside antibodies. anti-gm1 and anti-gd1a antibodies reacted with the lpss of pen 1, 4, and 19. aspinall et al. (g. o. aspinall, a. g. mcdonald, t. s. raju, h. pang, a. p. moran, and j. l. penner. eur. j. biochem. 213:1017-1027, 1993) recently reported t ...19947513313
molecular mimicry between gq1b ganglioside and lipopolysaccharides of campylobacter jejuni isolated from patients with fisher's syndrome.we isolated campylobacter jejuni from 2 patients with fisher's syndrome subsequent to enteritis. crude lipopolysaccharide fractions were extracted from the bacteria and separated by thin-layer chromatography. monoclonal antibodies to gq1b ganglioside (gmr13 and 7f5) reacted with both lipopolysaccharide fractions, indicating that the lipopolysaccharides bear the gq1b epitope. this is the first report of molecular mimicry between neural tissue components and the antecedent infectious agents of fis ...19947526777
isotype, specificity, and kinetics of systemic and mucosal antibodies to campylobacter jejuni antigens, including flagellin, during experimental oral infections of chickens.the immune response of chickens to campylobacter jejuni infection was studied as a step in the search for vaccine candidates. one-day-old chicks orally challenged with c. jejuni strain 81116 showed significant increases in specific igg, iga, and igm circulating antibodies, as detected by enzyme-linked immunosorbent assay (elisa). these levels peaked at 9, 5, and 7 weeks postinfection, respectively. maternal igg antibodies were also detected over the first 2 weeks. specific mucosal igg and iga an ...19947526839
isolation and characterization of the flagellar hook of campylobacter jejuni.a method for purification of the flagellar hook of campylobacter jejuni is described. the hook was shown to be composed of a subunit protein, which has a molecular mass of 92,000 and an isoelectric point of pi 4.8. a monoclonal antibody and a polyvalent antiserum was raised against the purified flagellar hook of c. jejuni. immuno-electronmicroscopy revealed that the epitope recognized by the monoclonal antibody is surface-located. however, this antibody reacted only with the hook of the immuniza ...19947527365
identification and characterization of an intervening sequence within the 23s ribosomal rna genes of campylobacter jejuni.campylobacter jejuni is a significant cause of bacterial enteritis in humans. three of seven c. jejuni isolates examined were found to contain fragmented 23s rrna. the occurrence of fragmented 23s rrna correlated with the presence of an intervening sequence (ivs) within the 23s rrna genes. the ivs is 157 nucleotides in length and replaces an eight nucleotide sequence in the 23s rrna genes of c. jejuni isolates that contain intact 23s rrna. the two ends of the ivs share 31 bases of complementarit ...19947530317
serum anti-gq1b igg antibodies recognize surface epitopes on campylobacter jejuni from patients with miller fisher syndrome.three patients who had diarrhea prior to the development of miller fisher syndrome are presented. campylobacter jejuni was isolated from stool specimens from all patients. high titers of anti-gq1b igg antibodies were demonstrated in the serum of these patients by enzyme-linked immunosorbent assay and thin-layer chromatography overlay. in enzyme-linked immunosorbent assay inhibition studies the anti-gq1b igg antibodies bound specifically to whole bacteria of the miller fisher syndrome-associated ...19957531419
ribosomal rna patterns identify additional strains of campylobacter jejuni and c. coli among isolates serotyped by heat-stable and heat-labile antigens.heat-stable (hs, o-antigen) and heat-labile (hl) serotyping are the most common methods used to type campylobacter jejuni and c. coli for epidemiologic purposes. in this study, we conducted rrna analysis to differentiate strains of c. jejuni and c. coli that had been serotyped by use of the passive hemagglutination (heat-stable) and slide agglutination (heat-labile) methods. ribotyping of isolates within hs and hl serotypes revealed further discrimination of strains. four ribotypes were identifi ...19947534848
safety and immunogenicity of a prototype oral whole-cell killed campylobacter vaccine administered with a mucosal adjuvant in non-human primates.the safety and immunogenicity of two prototype oral campylobacter killed whole-cell (cwc) vaccines were tested in rhesus monkeys. animals were immunized with a primary two-dose series (days 0 and 14) of vaccine consisting of cwc (10(10) particles/dose) given alone or in combination with 0.5-1000 micrograms of the heat-labile enterotoxin of escherichia coli as an oral adjuvant (oa). a booster vaccination, 4 weeks after primary immunization, was given to animals receiving cwc alone or supplemented ...19957539199
expression of adhesion molecules on human granulocytes after stimulation with helicobacter pylori membrane proteins: comparison with membrane proteins from other bacteria.type b gastritis in its active form is characterized by a dense infiltration of the lamina propria with granulocytes. since the bacterium helicobacter pylori does not invade the epithelial barrier, a signaling pathway chemoattractive for granulocytes must exist across this mucosal boarder. one possible mechanism tested was whether granulocytes are directly activated by water-soluble membrane proteins (wsp) from h. pylori. these findings were compared with the effects of wsp from other bacteria ( ...19957540595
molecular subtyping scheme for serotypes hs1 and hs4 of campylobacter jejuni.we describe a molecular subtyping scheme for two principal o (heat-stable [hs]) serotypes of campylobacter jejuni, hs1 and the hs4 complex. a 16s rrna gene-specific probe confirmed that almost all the c. jejuni strains had three copies of this gene, and strains could be assigned with complete typeability to 1 of 16 combined (pst1 and haeiii) 16s ribotypes. macrorestriction profiles (mrps) consisting of up to 10 smai fragments from approximately 40 to approximately 480 kbp were resolved by pulsed ...19957540628
conformational analysis of the campylobacter jejuni porin.the major outer membrane protein (momp) of campylobacter jejuni was purified to homogeneity by selective solubilization and fast protein liquid chromatography. the amino acid composition of the momp indicates the presence of cysteine residues. the amino-terminal sequence, determined over 31 residues, shows no significant homology with any other porin from gram-negative bacteria except in a discrete region. immunocross-reactivity between escherichia coli ompc and the momp was analyzed, and a comm ...19957543469
subclass of igg antibody to gm1 epitope-bearing lipopolysaccharide of campylobacter jejuni in patients with guillain-barré syndrome.sera of patients who develop guillain-barré syndrome (gbs) subsequent to campylobacter jejuni enteritis frequently have igg anti-gm1 antibody. lipopolysaccharide (lps) of c. jejuni isolated from a gbs patient has a gm1 ganglioside-like structure. igg subclass distribution of the anti-gm1 antibody in gbs patients is mainly restricted to igg1 and igg3. since igg antibodies to bacterial polysaccharide generally are restricted to igg2 subclass, some investigators have assumed that either the general ...19957543907
chemical structures of the core region of campylobacter jejuni o:3 lipopolysaccharide and an associated polysaccharide.the complete structure for the core oligosaccharide region of the water-insoluble low-m(r) lipopolysaccharide of campylobacter jejuni serotype o:3 from phenol/water extraction of bacterial cells was assigned through studies on derivatives of the liberated oligosaccharide. structure determinations were performed using 1h-nmr and 31p-nmr spectroscopies, methylation analysis supported by fast-atom-bombardment mass spectrometry, and smith degradation experiments. it was concluded that the complete c ...19957544281
ganglioside-like epitopes of lipopolysaccharides from campylobacter jejuni (pen 19) in three isolates from patients with guillain-barré syndrome.sera from patients with guillain-barré syndrome (gbs) frequently have anti-gm1 antibody. we earlier showed that an lipopolysaccharides (lps) from campylobacter jejuni (pen 19) isolated from a gbs patient has a gm1 ganglioside-like structure. aspinall et al. (biochemistry, 61 (1994) 335-337) reported that oh 4382 has an lps that bears a cd3 ganglioside-like structure and that oh 4384 has an lps that bears a gt1a-like structure; both strains were isolated from patients with gbs. they also suggeste ...19957544402
invasion and viability of campylobacter jejuni in experimentally contaminated japanese quails' eggs.in japanese quail eggs experimentally immersed in a suspension of campylobacter jejuni from human patients (y6817, y6878), strain y6817 was recovered from 15 (18.8%) eggs among 80 between 0.5 hr and 72 hr after immersion. invasion of the eggs by strain y6878 was also seen in 10 (12.5%) of 80 eggs between 0.5 hr and 24 hr after immersion. when egg yolk was inoculated with both strains and held at 4 degrees c, the organisms were detectable for a longer time than the ones kept at 20 degrees c. on t ...19957548430
detection and identification of campylobacter coli and campylobacter jejuni by two-step polymerase chain reaction.flagellin gene was used as target sequence to detect and distinguish c. coli and c. jejuni by a "nested pcr" technique. the method shows a high level of sensitivity and specificity. application of this rapid diagnostic tool could provide further information about epidemiological and pathogenetic implications of each of these two microorganisms.19957552696
comparison of preservation media for storage of stool samples.transportation of clinical samples and long-term recoverability of pathogens are critical to epidemiological studies, particularly when conditions do not permit immediate processing. this study confirms that cary-blair medium (cb) is suitable for the preservation of salmonella and shigella isolates for more than 2 weeks at 25, 4, or -70 degrees c. campylobacter jejuni was not recovered after 2 days of storage in cb at 25 degrees c when an inoculum of 12 x 10(8) cells per ml was used. lower tempe ...19957559972
high-resolution genotyping of campylobacter coli identifies clones of epidemiologic and evolutionary significance.campylobacter coli strains from clinical and other sources were examined in terms of o (heat-stabile; hs) serotype and by several molecular typing techniques. restriction fragment length polymorphism (rflp) around the three 16s rrna genes revealed 10 variants, none found in campylobacter jejuni. rflp analysis of a polymerase chain reaction amplicon generated from the flagellin gene (flaa) yielded 11 polymorphism groups, some of them linked to hs serotypes. enlarged flaa genes, contributing three ...19957561196
cytotoxin detection in campylobacter jejuni strains of human and animal origin with three tissue culture assay systems.cytotoxin (ctx) production in 34 human and 22 animal strains of campylobacter jejuni isolated in japan and other countries was studied by three assay systems described previously. furthermore, cholera-like enterotoxin production by these strains was tested by reversed passive latex agglutination (rpla). ctx titres in the fetal calf serum (fcs) and newborn calf serum (ncs) assays were relatively lower, with a maximum of 4 and 8, respectively, than the maximum of 128 for the serum-free culture (sf ...19957563000
dna diversity among isolates of campylobacter jejuni detected by pcr-based rapd fingerprinting.a pcr-based randomly amplified polymorphic dna method was used to amplify campylobacter jejuni dna using a single oligonucleotide primer derived from either a homologous source or from mycoplasma gallisepticum. the method was able to detect the heterogeneity of amplified dna from human, chicken and turkey sources and can be used as a tool to study the epidemiology of campylobacter jejuni infection.19957571378
analysis of flagellin gene expression in flagellar phase variants of campylobacter jejuni 81116.flagella production in campylobacter jejuni 81116 is subject to phase variation; the bacterium is able to switch its flagellum synthesis, and thereby its motility, on and off. under standard laboratory growth conditions flagellar phase variants can be maintained as stable, pure cultures. we found conditions that efficiently induced a phase shift in vitro. the flaa gene but not the flab gene is subject to the on and off switch. minor amounts of flab are still present in aflagellate cells. we prev ...19957574555
survival of campylobacter jejuni in foods and comparison with a predictive model.campylobacter jejuni was inoculated into a range of raw and cooked foods and survival determined during storage at 2 degrees, 10 degrees and 20 degrees c for up to 56 d. to facilitate easy enumeration, two antibiotic-resistant strains of camp. jejuni, which had similar survival characteristics to the parent strain, were used. campylobacter jejuni survived for longer at lower temperatures in all foods and inactivation was most rapid in pâté. there was generally good agreement between the survival ...19957576507
effect of the complement system on the sensitivity of campylobacter jejuni and campylobacter coli to human blood serum.we determined the sensitivity of five strains of campylobacter jejuni and c. coli isolated from children with diarrhea and from chicken feces to normal human blood serum (undiluted and at concentrations of 10, 30, 50 and 70%), a hypogammaglobulinemic serum and a complement-deficient serum. both species were highly sensitive to the bactericidal activity of human serum, regardless of their source. the highest bactericidal activity was observed with pooled fresh normal serum, with bacterial surviva ...19957581046
physical and genetic map of the genome of campylobacter upsaliensis.a physical map of campylobacter upsaliensis atcc 43954 was constructed from dna fragments generated by sali (5' g/tcgac), nari (5' gg/cgcc) and bsshii (5' g/cgcgc) restriction digests separated using pulsed-field gel electrophoresis. the size of the c. upsaliensis genome was approximately 2000 kb, providing evidence of the largest campylobacter genome sized to date. twenty-one fragments created from these restriction digests were assembled into a physical map using a combination of complementary ...19957582001
an isoleucyl-trna synthetase gene from campylobacter jejuni.a complete isoleucyl-trna synthetase gene (iles) of campylobacter jejuni was isolated from a c. jejuni tgh9011 genomic dna library constructed in pbluescript. the complete coding sequence, flanking regions and transcription start point were determined. the deduced isoleucyl-trna synthetase (ilers) had 917 amino acids with a molecular mass of 105,399 da, which was consistent with the observed size of 105 kda in escherichia coli maxicells. the iles gene was mapped onto the physical map of the c. j ...19957582016
detection of genomic variability among isolates of campylobacter jejuni from chickens by crossed-field gel electrophoresis.digestion with sal i facilitated the subclassification of 41 strains of campylobacter jejuni into seven types, and digestion with sma i enabled subclassification into twelve types. sma i was potentially more useful for the detection of variability among the 41 strains, but both restriction enzymes seemed to be potentially useful for detecting variability among crossed-field gel electrophoresis profiles of the strains. the results clearly demonstrated that c. jejuni strains from different sources ...19957587375
comparison of two selective media and a membrane filter technique for isolation of campylobacter species from diarrhoeal stools.diarrhoeal stool specimens from 415 patients were examined for campylobacter spp. by culture on charcoal cefoperazone deoxycholate agar (ccda), skirrow medium and columbia blood agar overlaid with a 0.65 micron pore size membrane filter. forty-eight campylobacter strains were isolated from 45 (10.8%) specimens by all media; 44 were campylobacter jejuni (91.7%), three were campylobacter coli (6.3%) and one was campylobacter hyointestinalis (2.0%). the percentages of campylobacter-positive specime ...19957588831
comparison of pfge, ribotyping and phage-typing in the epidemiological analysis of campylobacter jejuni serotype hs2 infections.in this study we have evaluated the ability of three typing methods, pulsed field gel electrophoresis (pfge), phage-typing and ribotyping, to discriminate not only between strains of differing serotypes but also between strains within a single serotype, heat stable serotype 2 (hs2). forty-five isolates derived from cases of campylobacter enteritis occurring in the cardiff area were examined. these included 18, mostly hs2, strains associated with an outbreak. the typing results for these and a fu ...19957589261
complete sequences and organization of the rrna operon from campylobacter jejuni tgh9011 (atcc43431).the rrna ribosomal rna (rrna) operon of campylobacter jejuni (cj) tgh9011 (atcc43431) was cloned and sequenced to completion. rrnas were then characterized by primer extension and s1 nuclease mapping analysis. the secondary structure models of cj 16s and 23s rrnas were constructed, and the models were compared to the corresponding models from other eubacterial rrna. the analysis presented a typical 5'-promoter-16s-trnas-23s-5s-terminator-3' prokaryotic rrna operon structure. however, an unusual ...19957590296
cloning and transcription regulation of the ferric uptake regulatory gene of campylobacter jejuni tgh9011.a campylobacter jejuni (cj) tgh9011 (atcc 43431) gene homologous to the escherichia coli ferric uptake regulatory gene (fur) has been cloned and characterized. cj fur encodes a polypeptide consisting of 157 amino acids (aa) (18.1 kda). the 5'-flanking region of the cj fur gene contains two putative catabolite activator protein (cap)-binding sequences and four fur boxes or fur-binding sequences (fbs), implicating camp and autogenous regulation respectively. a major and a minor transcription start ...19957590316
campylobacter jejuni motility and invasion of caco-2 cells.we investigated the influence of motility on campylobacter jejuni binding and invasion of caco-2 cells. c. jejuni was motile in soft agar at basic (ph 8.5) and neutral ph values representative of the intestinal environment. however, c. jejuni was immobilized at ph 5.0. the inability of c. jejuni to swarm on soft agar at ph 5.0 was not related to flagellar depolymerization or loss of viability. in tissue culture medium, c. jejuni displayed typical periods of straight swimming punctuated by tumbli ...19957591061
acute cholecystitis caused by campylobacter jejuni.an 83-year-old man with acute cholecystitis caused by campylobacter jejuni is described. the patient was cured after undergoing cholecystectomy and intravenous ofloxacin therapy.19957591706
cytotoxin production by campylobacter jejuni isolated from turkish patients with diarrhoea. 19957594315
electrophysiological studies in guillain-barré syndrome: correlation with antibodies to gm1, gd1b and campylobacter jejuni.a retrospective study of 50 patients with guillain-barré syndrome (gbs) correlated analysis of serial motor nerve conduction studies with the presence of antibodies to campylobacter jejuni, gm1 and gd1b, determined by elisa. gbs patients with antibodies to c. jejuni (n = 8), gm1 (n = 4), or gd1b (n = 4) showed electrophysiological features suggestive of demyelination with prolonged distal motor latencies and temporal dispersion/conduction block similar to gbs patients without these specific anti ...19957595678
first isolation of campylobacter jejuni from the vaginal discharge of three bitches after abortion in south africa.campylobacter jejuni was isolated in pure culture from the vaginal discharge from three german shepherd bitches after late-pregnancy abortions. the main clinical sign occurring in the bitches was a profuse and odourless haemorrhagic vaginal discharge.19947596570
guillain-barré syndrome in northern china. relationship to campylobacter jejuni infection and anti-glycolipid antibodies.guillain-barré syndrome has been considered to be primarily an acute inflammatory demyelinating polyneuropathy (aidp). our experience with guillain-barré syndrome in northern china differs from the traditional concept. electrophysiologically and pathologically, most of our patients have motor axonal degeneration with minimal cellular inflammation, which we have termed 'acute motor axonal neuropathy' (aman). the current studies were undertaken to characterize prospectively the clinical, electroph ...19957600081
two novel restriction endonucleases from campylobacter jejuni.we have discovered two unusual restriction endonuclease (enases) in two campylobacter jejuni strains that recognize asymmetrical, interrupted sequences and cleave the dna both before and after their recognition sites. both enzymes require adomet as a cofactor for their enase activity.19957607469
campylobacter jejuni in the stomach.campylobacter jejuni is the commonest cause of acute bacterial enteritis in the uk. however, in this case a 74-year-old lady underwent gastroscopy for an upper gastrointestinal haemorrhage and was noted to have a gastric ulcer. gastric biopsy revealed spiral gram-negative bacteria and culture yielded a moderate growth of c. jejuni. identification was confirmed by growth characteristics, biochemical tests and pcr amplification of the species-specific flagellin gene--fla a. to prevent misidentific ...19957608961
[a case of guillain-barré syndrome with ophthalmoplegia showing high titers of anti-gq1b and -gd1b antibodies in the serum].a 24-year-old housewife developed double vision, tingling sensation, and weakness in the legs following a flu-like illness. she then developed dysphagia and difficulty in standing. on admission her eyes were fixed in midline due to ophthalmoplegia. doll's eye sign was negative. she had severe generalized muscle weakness and no sensory deficits. all tendon reflexes were lost. csf protein was 58 mg/dl with cell count of 2/mm3. antibodies to campylobacter jejuni, mycoplasma, ebv, and other microbes ...19957614756
[fisher's syndrome following campylobacter jejuni enteritis--a case report and review of the literature].a 13-year-old boy presented with ophthalmoplegia, cerebella ataxia and areflexia subsequent to watery diarrhea and pyrexia. campylobacter jejuni (pen 2: lio 4) was isolated from the stools. the patient had a high titer of igg anti-gq1b igg antibody titers which decreased during the clinical course. although c. jejuni has recently been recognized as the most common microorganism causing infection preceding guillain-barré syndrome, only six patients with fisher's syndrome after this bacterial infe ...19957614765
differentiation of campylobacter jejuni and campylobacter coli strains by using restriction endonuclease dna profiles and dna fragment polymorphisms.the chromosomal dna fragment patterns from a total of 169 campylobacter jejuni and campylobacter coli isolates from poultry and humans were analyzed by using dna restriction endonucleases clai and ecorv. the dna restriction patterns produced by clai and ecorv consisted of unique dna fragments of 9 to 9.5 kb and 3.5 kb generated with clai and a single unique fragment of 3.0 kb produced by ecorv. these patterns were obtained with all strains of c. jejuni tested. the dna restriction patterns were f ...19957615717
temperature-dependent membrane fatty acid and cell physiology changes in coccoid forms of campylobacter jejuni.the effect of temperature and the availability of nutrients on the transition of spiral campylobacter jejuni cells to coccoid forms was investigated. ageing of spiral c. jejuni cells in either nutrient-poor or nutrient-rich environments resulted in the formation of nonculturable coccoid cells at 4, 12, and 25 degrees c after different periods, with the cells incubated at 4 degrees c in nutrient-deficient media remaining culturable the longest. to study the phenomenon, atp levels, protein profile ...19957618883
campylobacter jejuni and the expanding spectrum of related infections. 19957619982
anti-ganglioside antibodies in patients with guillain-barré syndrome and campylobacter jejuni infection. 19957622918
[comparison by ses-page of molecular weights of lipopolysaccharides from campylobacter jejuni lior serotype reference strains and clinical isolates].to compare the molecular weights (mws) of lipopolysaccharides (lpss) from 30 lior serotype reference strains and 17 clinical isolates of campylobacter jejuni, we analyzed their migration rates by sds-page and the silver staining of the gel. lpss from the serotype strains showed one band in the low-molecular-weight region of the gel as did those from r mutants of enterobacteria. based on those from salmonella minnesota r mutants, mws of lpss from c. jejuni strains were calculated to fall within a ...19957623421
biological and serological characterization of campylobacter jejuni lipopolysaccharides with deviating core and lipid a structures.lipopolysaccharides from campylobacter jejuni were tested for their ability to induce toxic lethality in galactosamine-sensitized mice, pyrogenicity in rabbits and tumour necrosis factor (tnf) secretion from mouse peritoneal macrophages. compared with those of salmonella lps, lethal toxicity was 50% lower, pyrogenicity was 30- to 50-fold lower, and ability to induce tnf was 100-fold lower. c. jejuni lps and lipid a exhibited higher phase-transition temperatures than those of salmonella preparati ...19957640672
immunogenicity and protective efficacy of a prototype campylobacter killed whole-cell vaccine in mice.the immunogenicity and efficacy of an experimental inactivated campylobacter jejuni whole-cell (cwc) vaccine were evaluated in mice. mice were orally immunized in a three-dose primary series (48-h intervals) at doses of 10(5), 10(7), or 10(9) cwc vaccine particles alone or in combination with 25 micrograms of a mucosal adjuvant, the heat-labile enterotoxin of escherichia coli (lt). the comparative immunogenicities of both formulations were assessed on the basis of the generation of antigen-speci ...19957642317
purification and characterization of ferritin from campylobacter jejuni.we purified an iron-containing protein from campylobacter jejuni using ultracentrifugation and ion-exchange chromatography. electron microscopy of this protein revealed circular particles with a diameter of 11.5 nm and a central core with a diameter of 5.5 nm. the protein was composed of a single peptide of 21 kda and did not serologically cross-react with horse spleen ferritin. the uv-visible spectrum of the protein showed no absorption peaks in the visible region, indicating that little or no ...19957646314
[infectivity of campylobacter jejuni and campylobacter coli in chickens].the infectivity of four campylobacter (c.) jejuni strains and one c. coli strain was evaluated by the reisolation rate from cloacal swabs after oral infection of chickens at four weeks of age. one strain did not colonize the digestive tract, another infected only a part of the experimental group and a third was excreted at a lower percentage in the first two weeks p.i. compared to the other two strains. in correlation to age, the excretion rate was highest in 7-week-old birds. in groups of 4 and ...19957646423
[epidemiological investigation of campylobacter jejuni infection in children].epidemiological studies on campylobacter jejuni infection in children were carried out in chengdu during december 1987 to october 1989. detection rate of campylobacter in 1,092 children with diarrhea was 11.36%, with the highest in children aged 1-2 and the lowest in babies within six months. campylobacter jejuni could be detected in young children with acute diarrhea all the year round, with a higher detection rate in the spring and summer and lower in the autumn and winter. campylobacter jejun ...19957648948
rapid detection of campylobacter jejuni and campylobacter coli isolated from clinical specimens using the polymerase chain reaction.seventeen campylobacter strains isolated from 16 children hospitalised with acute diarrhea were analysed by in vitro enzymatic amplification using two sets of oligonucleotide primers specific for campylobacter jejuni and campylobacter coli, respectively. thirteen strains (76%) were identified as campylobacter jejuni and four strains (24%) as campylobacter coli. subsequent bacteriological identification confirmed the identity of the same 13 campylobacter jejuni strains and the 4 campylobacter col ...19957649203
[the complement fixation reaction: a traditional, updated method for the detection of antibodies in the diagnosis of infections].complement-fixation (cf) is still an important basic serologic test for the diagnosis of infectious diseases. in several areas of microbiology (viral, bacterial, parasitic and fungal) it has served as a reference standard against which other methods have been compared. its partial displacement by other techniques, as advocated in some recent literature, is often unfounded and uncritical; displacement is mostly due not to problems inherent in the method, but rather to the lack of reagents of sati ...19957651069
anti-gm1 igg antibodies and campylobacter bacteria in guillain-barré syndrome: evidence of molecular mimicry.in guillain-barré syndrome antibodies to gm1 and the presence of an antecedent campylobacter jejuni infection are correlated with a more severe course of the disease. from a group of 137 consecutive gbs patients, 11 sera had elevated titers of anti-gm1 igg antibodies during the acute stage of disease. each serum sample was preincubated with three different penner serotypes of whole c. jejuni (pen o:4/59, pen o:41) and campylobacter coli (pen o:22) bacteria. the pen o:4/59 serotype, isolated from ...19957654064
guillain-barré syndrome without sensory loss (acute motor neuropathy). a subgroup with specific clinical, electrodiagnostic and laboratory features. dutch guillain-barré study group.we analysed data obtained from 27 out of a group of 147 patients with guillain-barré syndrome, who did not have sensory loss during a follow-up period of 6 months (motor guillain-barré syndrome). these patients had a distinctive clinical pattern compared with the other 120 guillain-barré syndrome patients. the clinical course was marked by a more rapid onset of weakness (3.9 versus 6.1 days, p = 0.002), an earlier nadir (6.3 versus 9.1 days, p < 0.001), an initially predominant distal weakness ( ...19957655882
a survey of campylobacter bacteremia in three danish counties, 1989 to 1994.over a 5-year period (april 1989 to march 1994) bacteremia caused by campylobacter species was diagnosed in 15 patients in a population of 1.3 million in three counties in jutland province, denmark. the incidence was 0.2/100,000/year and the ratio of blood to fecal isolates, was 0.008. campylobacter coli and campylobacter jejuni accounted for 6 cases each, campylobacter fetus for 2, and in 1 case the species diagnosis was uncertain. seven patients were males and 8 females; median age was 42 (ran ...19957660078
multiple abscesses of the liver caused by campylobacter jejuni.a 37-year-old woman developed multiple liver abscesses caused by campylobacter jejuni, as a consequence of unrecognized and inadequately treated campylobacter enteritis. the diagnosis was established by isolation of campylobacter jejuni from blood and pus obtained from one of the liver abscesses during laparoscopy. the abscesses were successfully treated with intravenously and orally administrated antibiotic drugs, without further percutaneous drainage.19957665820
sepsis in children with human immunodeficiency virus infection. the madrid hiv pediatric infection collaborative study group.the aims of this retrospective study were to review the frequency and patterns of bacterial sepsis in children infected with human immunodeficiency virus. the charts of 233 human immunodeficiency virus-infected children cared for during a 10-year period in 4 tertiary hospitals in madrid were reviewed. there were 43 episodes of sepsis in 31 (13%) children. twenty of them had acquired immunodeficiency syndrome, 10 were class pa2 and 1 was class p1b. the most common organisms recovered were: nontyp ...19957667058
the gene for campylobacter trigger factor: evidence for multiple transcription start sites and protein products.a gene encoding a protein of apparent molecular mass 56 kda that shares 31% identity with the amino acid sequence of trigger factor from escherichia coli (a protein thought to be involved in cell division), was cloned from campylobacter jejuni nctc 11168. the clone was selected from a lambda zap ii genomic dna library following an immuno-screen using antiserum raised against glycine-extractable proteins from c. jejuni. the gene has two potential initiation codons, giving rise to two possible nes ...19957670637
molecular characterization of kata from campylobacter jejuni and generation of a catalase-deficient mutant of campylobacter coli by interspecific allelic exchange.a gene encoding catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase; ec 1.11.1.6) from campylobacter jejuni was cloned by functional complementation of a catalase-deficient mutant of escherichia coli. the catalase structural gene, designated kata, was assigned by subcloning and its nucleotide sequence determined. the deduced protein product of 508 amino acids, which had a calculated molecular mass of 58,346 da, was found to be structurally and enzymically similar to hydrogen-peroxidases ...19957670638
[reactive arthritis associated bacteria as the etiology of undifferentiated oligoarthritis].undifferentiated oligoarthritis (uoa) resembles clinically reactive arthritis (rea), but does not fulfill the diagnostic criteria. in 46 patients with uoa, in 16 with rea, and in 15 with rheumatoid arthritis (ra) the humoral and cellular immune response to the rea-associated bacteria chlamydia trachomatis, yersinia enterocolitica, shigella flexneri, salmonella enteritidis, campylobacter jejuni and borrelia burgdorferi was investigated in paired samples of synovial fluid and peripheral blood. an ...19937682746
common somatic o and heat-labile serotypes among campylobacter strains from sporadic infections in the united states.somatic o (formerly heat-stable) and heat-labile (hl) serotyping methods are commonly used to type campylobacter jejuni and campylobacter coli isolates. although both systems are effective, the labor and time required for each have limited their application. these systems can be simplified by reducing the number of antisera used. to find an appropriate panel of antisera, we determined the distribution of common serotypes in the united states among a representative sample of 298 campylobacter iso ...19937686183
the epidemiology of bacterial resistance to quinolones.the new fluoroquinolones have been in use for nearly 10 years in the treatment of community- and nosocomially-acquired infections. resistant clones may be selected during therapy and disseminate if favourable epidemiological conditions prevail. resistance to the fluoroquinolones is still rare in common pathogens with 97 to 100% of strains remaining susceptible. resistance has been reported in methicillin-susceptible staphylococcus aureus, campylobacter jejuni/coli, salmonella, shigella and esche ...19937689447
amplification and sequencing of variable regions in bacterial 23s ribosomal rna genes with conserved primer sequences.published bacterial 23s ribosomal rna sequences were aligned, and universally conserved regions flanking highly variable regions were looked for. in strategically positioned conserved regions, six oligonucleotides suitable for polymerase chain reaction (pcr) and sequencing were designed, allowing fast sequencing of four of the most variable 23s rrna regions. two other primers were designed for pcr amplification of nearly complete 23s rrna genes. all these primers successfully amplified fragments ...19937691306
[synovial cellular immune response to bacterial pathogens in patients with chronic juvenile arthritis].juvenile chronic arthritis is a heterogenous disease with an ill-defined pathogenesis. in our study, synovial fluid (sf) and peripheral blood (pb) of 70 children with oligoarthritis were investigated; bacteria-specific lymphocyte proliferation and antibodies to arthritogenic bacteria were determined. specific cellular immune responses in sf but not in pb were found in 4/7 patients with either lyme- or reactive arthritis (60%). in comparison, in subgroup jca ii (n = 45) encompassing mainly elder ...19937692686
demonstration of lipopolysaccharide with o-polysaccharide chains among different heat-stable serotypes of campylobacter jejuni by silver staining of polyacrylamide gels.lipopolysaccharide (lps) from three human and four monkey isolates of campylobacter jejuni was extracted in high yields that revealed ladder-like structures in polyacrylamide gels by direct silver staining. these observations demonstrate that c. jejuni possesses lps with o-chain repeating units typical of the family enterobacteriaceae. the isolates showed differences in the number and electrophoretic mobility of bands in silver-stained gels.19937693600
nucleotide sequence of the gyra gene and characterization of ciprofloxacin-resistant mutants of helicobacter pylori.pcr was used to amplify a 238-bp region from helicobacter pylori which corresponded to the quinolone resistance-determining region in escherichia coli. the gyra gene of h. pylori was cloned and sequenced. an open reading frame of 2,478 nucleotides coded for a polypeptide of 826 amino acids with a calculated molecular mass of 92,508 da. the amino acid sequence showed an overall 52% identity with other bacterial gyra genes but was most closely related to the gyra subunit of campylobacter jejuni (7 ...19957695290
[beta-lactamases of campylobacter spp. and their use as epidemiologic markers].the tests of 64 clinical strains of campylobacter jejuni and 69 strains isolated from hens revealed beta-lactamases in 96.99 per cent of the cultures. the detected enzymes more frequently splitted oxacillin and ampicillin among 5 betalactams used as the substrates (ampicillin, azlocillin, oxacillin, cefoperazon and cefotaxime). on the whole 24 spectra of the beta-lactamase activity with the evident domination of the amazoxcfpctx spectrum (29.69 per cent) were detected in both the clinical strain ...19947695448
adhesion activity of campylobacter jejuni for intestinal epithelial cells and mucus and erythrocytes.live and formalin- or heat (100 degrees c, 30 min)-treated cells of campylobacter jejuni strains y6878 and y6817, and the supernatant of the heat-treated bacterial suspension had agglutinating activity for the intestinal epithelial cells of japanese quails and human (int407). among the erythrocytes derived from 7 animal species (human, horses, sheep, rabbits, mice, chickens, and quails), only rabbit erythrocytes were agglutinated by live and glutaraldehyde-, formalin-, or heat-treated cells of 1 ...19947696403
cross-reactive antigens shared by pseudomonas aeruginosa, helicobacter pylori, campylobacter jejuni, and haemophilus influenzae may cause false-positive titers of antibody to h. pylori.cystic fibrosis (cf) patients suffer from many of the gastrointestinal conditions which occur in non-cf individuals, e.g., dyspepsia and peptic ulceration. these symptoms may be caused by helicobacter pylori but could also be due to either pancreatic insufficiency or the intensive antibiotic treatment used in cf patients. since cf patients chronically infected with pseudomonas aeruginosa produce antibodies against a wide range of antigens, including antigens common to many other bacteria, e.g., ...19957697522
ultrastructure of the bacteria campylobacter and helicobacter: implications for the phylogeny of mammal gastric bacteria.negative staining, thin sections, and surface replica methods were used to produce ultrastructural descriptions of curved-shaped, flagelated bacteria campylobacter jejuni, helicobacter pylori, and h. muridarum, the last one from the ileon of mice. the former has nude monotrichous flagella, the others have the sheated lobotrichous type. h. muridarum presents 10-12 periplasmic fibers. a cladogram of some gastric bacteria, based on the evolutionary history of the mammal hosts, hypothesizes that (1) ...19947701086
[epidemiology and etiology of infectious diarrheas. the case of mexico].gastrointestinal infections represent a health problem. it is estimated that 1647 million cases of diarrhea and 3.2 million deaths due to this cause occur among children less than five years of age per year. those belonging to this age group have 15 times more risk of dying because of diarrhea. cases of liquid acute diarrhea with blood represent 80% of cases, diarrhea with blood represent 10%. most frequent causes of liquid diarrhea are enterotoxigenic escherichia coli and rotaviruses and most f ...19947701141
occurrence of plasmids and tetracycline resistance among campylobacter jejuni and campylobacter coli isolated from whole market chickens and clinical samples.twenty whole market chickens, purchased from 10 different stores in the taipei metropolitan area, were examined for the presence of campylobacter jejuni and campylobacter coli. the microorganisms were recovered from 95% of the chickens. a survey of different sites on--breast, thigh and tail--showed that contamination was equally common on all these sites. one hundred and sixty-seven chicken isolates and the 41 clinical isolates of campylobacter jejuni were examined for the occurrence of plasmid ...19947703010
detection of the coccoid form of campylobacter jejuni in chicken products with the use of the polymerase chain reaction.detection of the coccoid form of campylobacter jejuni with the use of the polymerase chain reaction (pcr) was examined. coccoid cells of this pathogen, formed at different temperatures, showed different detection characteristics in the pcr. for spirals and cocci formed at 4 degrees c and 12 degrees c, the detection limit was about 2 x 10(3) cells/pcr. however, for detection of coccoid cells formed at 25 degrees c and 37 degrees c, at least 2 x 10(4) cells per pcr were needed. pcr was also perfor ...19947703020
[detection of imipenem resistance in a strain of campylobacter jejuni isolated from feces of a boy with diarrhea]. 19957711121
identification of helicobacter pylori by immunological dot blot method based on reaction of a species-specific monoclonal antibody with a surface-exposed protein.monoclonal antibodies (mabs) against membrane preparations of helicobacter pylori were produced. one mab was found to be specific for h. pylori, because it did not react with a number of other bacterial species, including helicobacter felis and campylobacter jejuni. this mab reacted with a 30-kda protein found in outer membrane preparations of h. pylori. the protein was also detected on the cell surface on intact bacteria when analyzed by immunoelectron microscopy. to facilitate the identificati ...19957714196
isolation of motile and non-motile insertional mutants of campylobacter jejuni: the role of motility in adherence and invasion of eukaryotic cells.a method of insertional mutagenesis for naturally transformable organisms has been adapted from haemophilus influenzae and applied to the study of the pathogenesis of campylobacter jejuni. a series of kanamycin-resistant insertional mutants of c. jejuni 81-176 has been generated and screened for loss of ability to invade int407 cells. eight noninvasive mutants were identified which showed 18-200-fold reductions in the level of invasion compared with the parent. three of these eight show defects ...19947715450
differential clinical and epidemiological features in children with campylobacter diarrhoea, mixed-agent diarrhoea and campylobacter diarrhoea plus parenteral infections.a prospective study of 111 young peruvian children with campylobacter jejuni diarrhoea showed that it behaves as an endemic enterotoxigenic-like, waterborne, milkborne, and zoonotic disease. although there were no definite differential features between pure c. jejuni diarrhoea, mixed-agent diarrhoea, and c. jejuni diarrhoea plus parenteral infections, children with c. jejuni diarrhoea plus parenteral infections were all inpatients, were more frequently malnourished and more frequently exhibited ...19957723135
susceptibilities of fluoroquinolone-resistant strains of campylobacter jejuni to 11 oral antimicrobial agents.the resistance of campylobacter jejuni strains to the fluoroquinolones is increasingly frequent, and in our area it reaches nearly 50%. we studied the susceptibilities of 60 of these strains to 11 oral antibiotics. all strains except one were susceptible to the macrolides tested, with azithromycin being the most active agent tested. of the rest of the antibiotics studied, amoxicillin-clavulanic acid, clindamycin, and fosfomycin displayed good in vitro activities. knowledge of the susceptibilitie ...19957726529
calcium and protein kinase c play an important role in campylobacter jejuni-induced changes in na+ and cl- transport in rat ileum in vitro.the pathophysiological mechanism of campylobacter jejuni (enterotoxigenic) induced secretory diarrhoea remains least understood. to investigate the mechanism(s) involved, the unidirectional fluxes of na+ and cl- were measured across the c. jejuni live culture infected and control (non infected) rat ileum (unstriped), in vitro by ussing technique under short circuit conditions, in the presence or absence of: ca2+ ionophore a23187 (5 microm), 1-verapamil (100 microm), calmodulin (cam) antagonist w ...19957727542
campylobacter jejuni infection and the guillain-barre syndrome: mechanisms and implications. 19947727903
interleukin-8 response of gastric epithelial cell lines to helicobacter pylori stimulation in vitro.gastric infection with helicobacter pylori activates a mucosal inflammatory response by mononuclear cells and neutrophils that includes expression of cytokines interleukin-1 beta (il-1 beta), il-6, tumor necrosis factor alpha, and il-8. in this study, we analyzed the il-8 response of human gastric cancer cell lines (kato iii, ags, and mkn28) to h. pylori infection in vitro. il-8 mrna expression was detected by reverse transcription-pcr amplification of rna extracted from epithelial cells after i ...19957729872
molecular characterization of a campylobacter jejuni lipoprotein with homology to periplasmic siderophore-binding proteins.a genomic library of campylobacter jejuni (nctc 11351) was used to identify genes which could confer a hemolytic phenotype to escherichia coli. accordingly, when transformants were screened on blood plates, hemolytic colonies appeared at a frequency of 3 x 10(-4). the gene conferring the hemolytic activity was identified by subcloning and was found to be responsible for the phenotype of all hemolytic transformants isolated. the open reading frame conferring this activity encodes a protein of 36, ...19957730251
expression and characterization of campylobacter jejuni benzoylglycine amidohydrolase (hippuricase) gene in escherichia coli.the basis for the difference between campylobacter jejuni and campylobacter coli is the presence and expression of the n-benzoylglycine amidohydrolase (hippuricase) gene only in c. jejuni. a pbr322 recombinant clone (phip-o) of c. jejuni tgh9011 capable of converting hippuric acid into benzoic acid and glycine, the hallmark of hippuricase activity, was characterized and sequenced. the hippuricase gene (hipo) was identified by use of deletion subclones and insertional inactivation. the transcript ...19957730270
igg anti-gm1 antibodies from patients with acute motor neuropathy are predominantly of the igg1 and igg3 subclasses.increased titers of igg anti-gm1 and anti-asialo gm1 (ga1) ganglioside antibodies are present in some patients with the guillain-barré syndrome, particularly with the motor axonal variant, and following infection with campylobacter jejuni or parenteral administration of gangliosides. the subclass distribution of igg anti-gm1 or ga1 antibodies from 19 patients with acute motor neuropathy and elevated antibody titers were measured by elisa using mouse monoclonal antibodies specific for human igg s ...19957730449
[pharmacokinetic, bacteriological and clinical studies of sy5555 in the pediatric field].pharmacokinetic, bacteriological and clinical studies on sy5555, a new oral penem, were carried out, and the following results were obtained. 1. mics were determined for 6 drugs, sy5555, clavulanic acid/amoxicillin (cva/ampc), cefaclor (ccl), cefotiam (ctm), cefpodoxime (cpdx), cefdinir (cfdn) against 20 strains of bacteria isolated from patients who were subsequently treated with sy5555. mics of sy5555 for gram-positive cocci ranged from 0.05 to 0.10 microgram/ml against 10 strains of staphyloc ...19957745814
detection of campylobacter jejuni added to foods by using a combined selective enrichment and nucleic acid sequence-based amplification (nasba).an assay to detect campylobacter jejuni in foods that uses a short selective enrichment culture, a simple and rapid isolation procedure, nasba amplification of rna, and a nonradioactive in solution hybridization was studied. the presence of high numbers of indigenous flora affected the sensitivity of the assay. however, detection of c. jejuni was possible up to a ratio of indigenous flora to c. jejuni of 10,000:1. interference by food components was eliminated by centrifugation following the enr ...19957747955
detection of alpha- and beta-hemolytic-like activity from campylobacter jejuni.alpha-hemolytic-like activity from campylobacter jejuni was clearly apparent when the medium ph ranged from 6.0 to 6.5, but the hemolytic zones disappeared when the ph of the medium increased. beta-hemolytic-like activity just beneath the bacterial growth appeared after prolonged incubation. the hemolytic activity was not influenced by the species of blood.19957751385
antimicrobial susceptibility and plasmid analysis of campylobacter jejuni isolated from diarrhoeal patients and healthy chickens in northern india.seventy-five strains of campylobacter jejuni isolated from humans with diarrhoea (45 strains) and healthy chickens (30 strains) were tested for their susceptibility to different antimicrobial agents: ampicillin, tetracycline, erythromycin, gentamicin, kanamycin, furazolidine and quinolones (nalidixic acid, norfloxacin, ciprofloxacin). the frequencies of resistance to ampicillin and tetracycline were 16 and 9.3% respectively. two strains (2.7%) exhibited resistance to quinolones as mentioned. one ...19947751568
polymerase chain reaction (pcr) for detection of pathogenic microorganisms in bacteriological monitoring of dairy products.the presence of pathogenic bacteria poses a serious problem in sustaining the safety of dairy products. microbiological routine controls of these products make use of selective culture techniques. to detect pathogenic species, isolated colonies are characterized by specific metabolic activities and by serotyping. we present an alternative biochemical approach that does not require culture of bacteria. the total bacterial populations of food samples were isolated by centrifugation and analysed by ...19957754231
specific detection of campylobacter jejuni by means of polymerase chain reaction in chicken litter.a routine detection assay of campylobacter jejuni was developed with the polymerase chain reaction (pcr). a pair of oligonucleotide primers from the flaa of c. jejuni were used in detection by pcr. although the primer pair specifically detected c. jejuni strains, they did not detect strains of c. coli, c. fetus, salmonella spp. or escherichia coli. in litter inoculated with c. jejuni, specific detection of c. jejuni was obtained by pcr with this primer pair.19957756404
development of a pcr assay combined with a short enrichment culture for detection of campylobacter jejuni in estuarine surface waters.two extraction procedures were examined, and it was found that dna recovered from campylobacter jejuni lysed by the cetyltrimethylammonium bromide (ctab) method was more suitable for use as a pcr template than dna released by the boiling method. the region targeted for pcr amplification was a 1.73-kb portion of the flagellin a gene of c. jejuni. the detection limit was lower than 30 cells per 100 ml in artificially contaminated waters. pcr assay and conventional culturing method had the same sen ...19957758934
resistance to erythromycin in fluoroquinolone-resistant campylobacter jejuni strains isolated from human faeces. 19957759401
[miller-fisher syndrome. role of campylobacter jejuni infection]. 19957761368
[infections caused by campylobacter jejuni in the pediatric department of the leoben district hospital 1983-1993].over the period 1983-1993 10,344 stool cultures were undertaken in children with gastrointestinal symptoms at the pediatric department of the landeskrankenhaus leoben. campylobacter jejuni was diagnosed as pathogen in 238 cultures taken from 196 patients aged one month to 16 years. thus, campylobacter jejuni infections (2.3% of all stool cultures) were second in frequency to various forms of salmonella (6.1% of all stool cultures; 332 patients). apart from diarrhea (92% of cases), the most frequ ...19957762243
morphological changes of campylobacter jejuni growing in liquid culture.campylobacter jejuni growing in liquid culture was found to exhibit gross morphological changes with time. exponentially growing cells showed typical short spiral forms. at mid-stationary phase the cells became approximately twice the length of the exponential forms. late stationary/early decline phase cells were seen to be a mixture of coccal forms and cells which were between 3 and 4 times the length of exponentially growing cells. continued incubation of cultures eventually resulted in a popu ...19937764089
a pcr assay for the detection of campylobacter jejuni and campylobacter coli in water.a pcr assay has been developed for the detection of campylobacter jejuni and camp. coli in water samples. the sample is filtered through a membrane which is subjected to sonication to release the impacted cells. after removal of the filter from the cell suspension and a freeze/thaw cell lysis step, a semi-nested pcr is carried out on the filtrate using the primers cf02, cf03 and cf04 (camp. jejuni flaa and flab gene sequences). incorporation of a sonication stage allows removal of the filter mem ...19947765441
inhibition of dnase activity in pfge analysis of dna from campylobacter jejuni.dnase-positive strains of campylobacter jejuni degrade their chromosomal dna during standard preparative procedures before pulsed-field gel electrophoresis (pfge). a simple method for inactivation of this dnase activity is described. formaldehyde fixation of the bacterial cells resulted in the preservation of the dna in a state suitable for restriction digestion and subsequent electrophoretic analysis.19947765449
isolation of the helicobacter pylori reca gene and involvement of the reca region in resistance to low ph.to understand the potential roles of the important dna repair protein reca in helicobacter pylori pathogenesis, we cloned the reca gene from h. pylori 84-183. degenerate pcr primers based on conserved reca protein regions were used to amplify a portion of h. pylori reca, which was used as a probe to isolate the full-length reca gene from h. pylori genomic libraries. the h. pylori reca gene encoded a protein of 347 amino acids with a molecular mass of 37.6 kda. as expected, h. pylori reca was hig ...19957768597
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