Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| subduing the hepatitis e python. | 2009 | 18796176 | |
| avian hepatitis e virus infection and possible associated clinical disease in broiler breeder flocks in hungary. | in broiler breeder flocks in one broiler integration in hungary, a new syndrome appeared in january 2005 with initially four successive post-peak flocks experiencing significant decreases in egg production. clinically birds became depressed and there was a small increase in the mortality rate. postmortem examinations revealed enlarged livers in up to 19% of birds dying, and enlarged spleens in some. also observed were birds with either clotted blood or serosanguineous fluid in the abdomen and su ... | 2008 | 18798029 |
| residues in the stalk domain of the hendra virus g glycoprotein modulate conformational changes associated with receptor binding. | hendra virus (hev) is a member of the broadly tropic and highly pathogenic paramyxovirus genus henipavirus. hev is enveloped and infects cells by using membrane-anchored attachment (g) and fusion (f) glycoproteins. g possesses an n-terminal cytoplasmic tail, an external membrane-proximal stalk domain, and a c-terminal globular head that binds the recently identified receptors ephrinb2 and ephrinb3. receptor binding is presumed to induce conformational changes in g that subsequently trigger f-med ... | 2008 | 18799571 |
| serological and molecular evidence of hepatitis e virus in swine in brazil. | active hepatitis e virus (hev) infections in two brazilian swine herds were investigated. in study 1, 26 piglets born to five anti-hev positive sows were monitored from birth to post-partum week 22. serum samples were screened for the detection of anti-hev antibodies and a nested rt-pcr used to examine the hev genome. passive transfer of immunity was confirmed. at week 22, 23/26 (88.4%) of the piglets had seroconverted. genome amplification was achieved in a feces pool from one holding pen and i ... | 2009 | 18805029 |
| [prevalence of antibodies against hepatitis e virus among students from india living in bialystok, poland]. | many evidences suggest hepatitis e virus (hev) infections may be more prevalent than previously described and viral hepatitis e cases can be observed in patients who have never visited endemic areas. the aim of this study was to assess the prevalence of serological markers of hev infection among students from india living in poland. | 2008 | 18807491 |
| crystal structure and carbohydrate analysis of nipah virus attachment glycoprotein: a template for antiviral and vaccine design. | two members of the paramyxovirus family, nipah virus (niv) and hendra virus (hev), are recent additions to a growing number of agents of emergent diseases which use bats as a natural host. identification of ephrin-b2 and ephrin-b3 as cellular receptors for these viruses has enabled the development of immunotherapeutic reagents which prevent virus attachment and subsequent fusion. here we present the structural analysis of the protein and carbohydrate components of the unbound viral attachment gl ... | 2008 | 18815311 |
| hepatitis e: does it cause chronic hepatitis? | hepatitis e virus (hev) is considered an agent responsible for acute hepatitis that does not progress to chronic hepatitis. we identified 14 cases of acute hev infection in three patients receiving liver transplants, nine receiving kidney transplants, and two receiving kidney and pancreas transplants. all patients were positive for serum hev rna. chronic hepatitis developed in eight patients, as confirmed by persistently elevated aminotransferase levels, serum hev rna, and histologic features of ... | 2008 | 18821611 |
| [new aspects of hev infection]. | the hepatitis e virus (hev), known for being the cause of major outbreaks of waterborne hepatitis in asia and africa, is an emerging pathogen in industrialized countries. virologic analyses of sporadic cases in europe, japan and the united states have shown that the origin of the infection was through autochthonous viral strains suggesting that the virus is present locally. in addition, transmission is not only enterically through contaminated water but also through ingestion of undercooked infe ... | 2008 | 18831406 |
| [rapid enterovirus genotyping in cerebrospinal fluids: a two-year prospective study in a virology laboratory setting]. | enterovirus (ev - 68 serotypes) infections comprise a wide spectrum of clinical presentations including infections of the central nervous system. in severe clinical presentation or epidemics, the precise identification of the involved serotype is necessary. | 2008 | 18835107 |
| induction of antibody response against hepatitis e virus (hev) with recombinant human papillomavirus pseudoviruses expressing truncated hev capsid proteins in mice. | a hepatitis e virus (hev) vaccine would be valuable to reduce the morbidity and mortality associated with the infection in endemic areas. hev pseudocapsids and epidermal delivery of hev orf2 dna vaccine by gene-gun have been shown to confer protection against virus challenge in monkeys. vectorization of a dna vaccine by virus-like particles is a new immunization approach. we report here the successful immunization of mice with two orf2 genes encapsidated into human papillomavirus type 31 virus-l ... | 2008 | 18835319 |
| mouse hepatitis virus liver pathology is dependent on adp-ribose-1''-phosphatase, a viral function conserved in the alpha-like supergroup. | viral infection of the liver can lead to severe tissue damage when high levels of viral replication and spread in the organ are coupled with strong induction of inflammatory responses. here we report an unexpected correlation between the expression of a functional x domain encoded by the hepatotropic mouse hepatitis virus strain a59 (mhv-a59), the high-level production of inflammatory cytokines, and the induction of acute viral hepatitis in mice. x-domain (also called macro domain) proteins poss ... | 2008 | 18922871 |
| a 20-year single-center experience with acute liver failure during pregnancy: is the prognosis really worse? | pregnant patients with acute liver failure (alf) are believed to have a worse outcome than nonpregnant women and men with alf. however objective data supporting this supposition are scant. therefore, the current study compared the outcome, complications, and causes of alf among pregnant women and girls with age-matched nonpregnant women and girls and men and boys with alf. one thousand fifteen consecutive alf patients in the reproductive age group, admitted at the all india institute of medical ... | 2008 | 18925633 |
| a pediatric patient with acute hepatitis e in japan. | we encountered a 14-year-old bangladeshi boy who developed acute hepatitis e in japan. he showed improvement without the development of fulminant hepatitis. his hepatitis e virus (hev) genotype was i, which causes epidemics mainly in south asia. he developed this disease more than 6 months after coming to japan. considering the latent period, it was suspected that he had been infected with hev in japan, although the hev virus is presumed not to be indigenous to japan. | 2008 | 18936892 |
| deletions of the hypervariable region (hvr) in open reading frame 1 of hepatitis e virus do not abolish virus infectivity: evidence for attenuation of hvr deletion mutants in vivo. | hepatitis e virus (hev) is an important human pathogen, although little is known about its biology and replication. comparative sequence analysis revealed a hypervariable region (hvr) with extensive sequence variations in open reading frame 1 of hev. to elucidate the role of the hvr in hev replication, we first constructed two hvr deletion mutants, hhvrd1 and hhvrd2, with in-frame deletion of amino acids (aa) 711 to 777 and 747 to 761 in the hvr of a genotype 1 human hev replicon. evidence of he ... | 2009 | 18945785 |
| peptide mimics of a conserved h5n1 avian influenza virus neutralization site. | a panel of 52 murine monoclonal antibodies was found to recognize antigenic determinants that had been conserved among all major genetic subgroups of the h5n1 avian influenza virus prevalent since 1997. we screened a phage display library for peptides recognized by one such antibody (8h5). we analysed the specificity of 8h5 for reactive peptides presented as fusion proteins of hbc (hepatitis b core protein) and hev (hepatitis e virus) structural protein, p239. this was then related to the specif ... | 2009 | 18973474 |
| genetic characterization of the complete coding regions of genotype 3 hepatitis e virus isolated from spanish swine herds. | the complete coding regions of five hepatitis e virus isolates of swine origin from two different pig farms and the complete genome sequence of two of these strains were obtained and compared to other full length or partial hev sequences. based on the nucleotide sequence, the examined spanish isolates were 87.1-99.7% similar among them being the closest known strain a mongolian porcine strain (swmn06-c1056) which shares 84.5-86.1% of the nucleotide sequence, and are also close to other hev porci ... | 2009 | 18977254 |
| of organ meats and hepatitis e virus: one part of a larger puzzle is solved. | 2008 | 18983247 | |
| phylogenetic and case-control study on hepatitis e virus infection in germany. | hepatitis e is a classic water-borne disease in developing countries. in germany, hepatitis e virus (hev) infections are notifiable. the number of non-travel-associated infections has increased in recent years, but the route of transmission in most is unknown. our objective was to determine risk factors for autochthonous hev infections in germany. | 2008 | 18983248 |
| differential activities of cellular and viral macro domain proteins in binding of adp-ribose metabolites. | macro domain is a highly conserved protein domain found in both eukaryotes and prokaryotes. macro domains are also encoded by a set of positive-strand rna viruses that replicate in the cytoplasm of animal cells, including coronaviruses and alphaviruses. the functions of the macro domain are poorly understood, but it has been suggested to be an adp-ribose-binding module. we have here characterized three novel human macro domain proteins that were found to reside either in the cytoplasm and nucleu ... | 2009 | 18983849 |
| neuralgic amyotrophy associated with hepatitis e virus. | neuralgic amyotrophy (na) is characterised by neuropathic pain and patchy paresis of the upper or lower limbs, usually involving the upper and middle trunks of the brachial plexus. the aetiology of na is varied, with precipitating factors that include trauma, surgery, pregnancy, inoculations and infections. deranged liver enzymes have been noted in previous na reports but no cause identified. we describe a case of bilateral na in a 53-year-old man who presented with peripheral neuropathy and iso ... | 2009 | 18986757 |
| hepatitis e: an emerging infection in developed countries. | hepatitis e is endemic in many developing countries where it causes substantial morbidity. in industrialised countries, it is considered rare, and largely confined to travellers returning from endemic areas. however, there is now a growing body of evidence that challenges this notion. autochthonous hepatitis e in developed countries is far more common than previously recognised, and might be more common than hepatitis a. hepatitis e has a predilection for older men in whom it causes substantial ... | 2008 | 18992406 |
| acute hepatitis e in south-west france over a 5-year period. | hepatitis e was found in people living in industrialized countries who had not travelled to highly endemic areas. | 2009 | 18993112 |
| retrospective serological study on hepatitis e infection in pigs from 1985 to 1997 in spain. | the objective of the present work was to ascertain the date in which hepatitis e virus (hev) was introduced in the spanish pig population. for this, a serological retrospective study was carried out using archived sera. a total of 2871 serum samples gathered between 1985 and 1997 and collected in 208 farms of spain were tested for anti-hev igg by an in-house elisa. of the 2871 sera analyzed by elisa, 1390 were positive for anti-hev antibodies (48.4%, 95% ci: 46.9-49.9%) and that corresponded to ... | 2009 | 18996653 |
| the yplgvg sequence of the nipah virus matrix protein is required for budding. | nipah virus (niv) is a recently emerged paramyxovirus capable of causing fatal disease in a broad range of mammalian hosts, including humans. together with hendra virus (hev), they comprise the genus henipavirus in the family paramyxoviridae. recombinant expression systems have played a crucial role in studying the cell biology of these biosafety level-4 restricted viruses. henipavirus assembly and budding occurs at the plasma membrane, although the details of this process remain poorly understo ... | 2008 | 19000317 |
| a mechanism to explain the selection of the hepatitis e antigen-negative mutant during chronic hepatitis b virus infection. | hepatitis b virus (hbv) expresses two structural forms of the nucleoprotein, the intracellular nucleocapsid (hepatitis core antigen [hbcag]) and the secreted nonparticulate form (hepatitis e antigen [hbeag]). the aim of this study was to evaluate the ability of hbcag- and hbeag-specific genetic immunogens to induce hbc/hbeag-specific cd4(+)/cd8(+) t-cell immune responses and the potential to induce liver injury in hbv-transgenic (tg) mice. both the hbcag- and hbeag-specific plasmids primed compa ... | 2009 | 19004949 |
| real-time monitoring of human enterovirus (hev)-infected cells and anti-hev 3c protease potency by fluorescence resonance energy transfer. | a real-time assay system that allows monitoring of intracellular human enterovirus (hev) protease activity was established using the principle of fluorescence resonance energy transfer (fret). it was accomplished by engineering cells to constitutively express a genetically encoded fret probe. the fret-based probe was designed to contain an enterovirus 71 3c protease (3c(pro)) cleavage motif flanked by the fret pair composed of green fluorescent protein 2 and red fluorescent protein 2 (dsred2). e ... | 2009 | 19015331 |
| hepatitis e, helicobacter pylori and peptic ulcers in workers exposed to sewage: a prospective cohort study. | workers exposed to sewage may have an increased risk of infection by helicobacter pylori and hepatitis e virus (hev). | 2009 | 19017699 |
| specific cellular immune response in hepatitis e patients. | to evaluate the specific t cell response together with igm anti-hepatitis-e-virus (hev) antibodies in acute hepatitis e (he) patients. | 2008 | 19018147 |
| hepatitis e virus as a newly identified cause of acute viral hepatitis during human immunodeficiency virus infection. | the recent description of chronic hepatitis e in organ transplant recipients deserves increased awareness in the context of hepatitis e virus (hev) infection in immunocompromised individuals. reported here is what is apparently the first pcr-documented case of acute hepatitis e in a human immunodeficiency virus (hiv)-1-infected patient. the cd4(+) t-lymphocyte count was 246/mm(3). the igm anti-hev antibody and hev rna tests results from serum were positive. hepatitis was benign, and chronic hev ... | 2008 | 19046170 |
| [hepatitis e: a third world's hepatitis found in belgium]. | hepatitis e virus is the second cause of acute viral hepatitis of oral-fecal origin in the world. this virus has a vast distribution throughout the world and manifests itself either in epidemic or endemic-sporadic form in many developing countries. usually, the cases of hev infection in industrialized countries are observed after a history of travel in an endemic area. however, an increasing number of cases have been attributed to a hev zoonotic form transmitted by swine. hev infection can lead ... | 2008 | 19051510 |
| detection of hepatitis e virus rna and genotype in bangladesh. | hepatitis e virus (hev) in bangladesh has not been adequately documented. we report hev rna and genotype detection in bangladesh. | 2009 | 19054262 |
| a novel mycovirus that is related to the human pathogen hepatitis e virus and rubi-like viruses. | previously, we reported that three double-stranded rna (dsrna) segments, designated l-, m-, and s-dsrnas, were detected in sclerotinia sclerotiorum strain ep-1pn. of these, the m-dsrna segment was derived from the genomic rna of a potexvirus-like positive-strand rna virus, sclerotinia sclerotiorum debilitation-associated rna virus. here, we present the complete nucleotide sequence of the l-dsrna, which is 6,043 nucleotides in length, excluding the poly(a) tail. sequence analysis revealed the pre ... | 2009 | 19073734 |
| [clinical features of acute hepatitis a in recent two years]. | the purpose of this study was to characterize the clinical features of acute hepatitis a in seoul and gyeonggi province during the recent 2 years. | 2008 | 19077476 |
| animal models of henipavirus infection: a review. | hendra virus (hev) and nipah virus (niv) form a separate genus henipavirus within the family paramyxoviridae, and are classified as biosafety level four pathogens due to their high case fatality rate following human infection and because of the lack of effective vaccines or therapy. both viruses emerged from their natural reservoir during the last decade of the 20th century, causing severe disease in humans, horses and swine, and infecting a number of other mammalian species. the current review ... | 2009 | 19084436 |
| [markers of hepatitis virus]. | hepatitis b virus (hbv) and hepatitis c virus (hcv) are the major viruses known to cause viral hepatitis. serological markers are commonly used as diagnostic and/or prognostic indicators of acute or chronic hbv or hcv infection. the ability to detect hbv dna in serum has been reported to have prognostic value for the outcome of chronic hbv infection. a rapid and sustained drop in hbv dna or hcv rna levels in patients under therapy has been shown to be a predictive factor for a favourable treatme ... | 2008 | 19086457 |
| challenge studies in rhesus monkeys immunized with candidate hepatitis e vaccines: dna, dna-prime-protein-boost and dna-protein encapsulated in liposomes. | complete orf2 gene (1983bp) of hepatitis e virus (hev) and the 450bp region within orf2 containing neutralizing epitope (ne) cloned in pvax1 and corresponding proteins expressed in baculovirus and prokaryotic systems respectively were evaluated as vaccine candidates. two doses of liposome encapsulated dna plus corresponding protein with both orf2 and ne regions (lipo-orf2-dp and lipo-ne-dp) showed 100% seroconversion and comparable anti-hev titres in swiss albino mice. these vaccine candidates w ... | 2009 | 19095027 |
| hepatitis e virus is highly prevalent among pregnant women in gabon, central africa, with different patterns between rural and urban areas. | hepatitis e virus (hev) is highly endemic in several african countries with high mortality rate among pregnant women. nothing is known about the circulation of this virus in central africa. we evaluated therefore the prevalence of anti-hev igg in samples collected from pregnant women living in the five main cities of gabon, central africa. we found that 14.1% (119/840) of pregnant women had anti-hev igg. the prevalence differed between regions and between age groups. in 391 newly collected sampl ... | 2008 | 19102767 |
| epidemiological screening for hepatitis e virus in bile specimens from livestock in northwest china. | hepatitis e has been hypothesized as a zoonosis. however, there is no definite conclusion about which animal species contribute to hepatitis e virus (hev) infection in humans. in this study, hev rna was detected only in swine bile specimens and not in bile specimens collected from cattle, goats, or dogs. we postulate that swine are the main animal reservoir for hev. | 2009 | 19109461 |
| hepatitis e virus genotype 3 diversity, france. | we characterized 42 hepatitis e virus (hev) genotype 3 strains from infected patients in france in 3 parts of the genome and sequenced the full-length hev genotype 3f genome found in europe. these strains are closely related to swine strains in europe, which suggests zoonotic transmission of hev in france. | 2009 | 19116067 |
| [hepatitis e: from zoonotic transmission to chronic infection in immunosuppressed patients]. | 2008 | 19116107 | |
| clinical significance of anti-hev iga in diagnosis of acute genotype 4 hepatitis e virus infection negative for anti-hev igm. | anti-hev igm is a diagnostic for recent or ongoing hev infection. however, some patients with acute hepatitis e (ahe) negative for anti-hev igm in acute period were often observed in clinical practice. in this study, we constructed the anti-hev iga indirect elisa assay to evaluate the significance of anti-hev iga. the specificity of anti-hev iga was 99.6%. among 245 ahe patients, 84 samples from 84 patients were positive for hev rna. the positive rate of anti-hev iga, anti-hev igm and anti-hev i ... | 2009 | 19117132 |
| a new artificial antigen of the hepatitis e virus. | an artificial antigen composed of 12 small antigenic regions derived from the orf2 and orf3 hev proteins was designed. the gene encoding for this artificial antigen was assembled from synthetic oligonucleotides by a new method called restriction enzyme-assisted ligation (real). the diagnostic relevance of this second generation hev mosaic protein (hev ma-ii) was demonstrated by testing this antigen against a panel of 142 well defined anti-hev positive and anti-hev negative serum samples. the dat ... | 2009 | 19117200 |
| the leu477 and leu613 of orf2-encoded protein are critical in forming neutralization antigenic epitope of hepatitis e virus genotype 4. | hepatitis e virus (hev) genotype 4 was originally identified in china. its neutralization antigenic epitopes have not been characterized. recently, we identified a neutralizing monoclonal antibody (mab) 1g10, which was generated following immunization of mice with p166chn, a recombinant protein comprising 464-629 amino acids (aa) of the hev genotype 4 capsid protein. in this study, a panel of 22 n- and/or c-terminal truncated and 6 site-directed mutated p166chn proteins were prepared. only those ... | 2008 | 19118511 |
| detection and genetic characterisation of hepatitis e virus in czech pig production herds. | the objective of the present study was to carry out a small surveillance programme in czech pig production herds using the nested reverse transcriptase-polymerase chain reaction (nrt-pcr) technique to trace hepatitis e virus (hev) in different biological samples and to characterise the detected swine hev isolates by phylogenetic analysis. a total of 32 piglets from 11 herds clinically suspected of postweaning multisystemic wasting syndrome (pmws) were examined. bile, liver tissue and serum sampl ... | 2009 | 19118850 |
| epidemiological study of hepatitis e virus infection in the general population of okinawa, kyushu, japan. | the aim of this study was to investigate the prevalence of hepatitis e virus (hev) infection in the general population of japan by determining presence of the antibody to hev (anti-hev). | 2008 | 19120876 |
| mice are not susceptible to hepatitis e virus infection. | to determine whether or not mice are susceptible to hepatitis e virus (hev) infection, c57bl/6 mice were experimentally infected with genotypes 1, 3 and 4 hev by intravenous injection. serum and stool samples were collected and used to detect hev rna and anti-hev antibodies by rt-pcr and elisa. the virus infection was monitored up to two months after inoculation; however, none of the serum or stool samples was positive for virus replication, demonstrating that c57bl/6 mice were not susceptible t ... | 2008 | 19122406 |
| determination of fecal shedding rates and genotypes of swine hepatitis e virus (hev) in korea. | hepatitis e virus (hev) infection induces an acute hepatitis or a subclinical disease in humans. it is known that hev is a zoonotic agent and pigs are major reservoirs of hev. this study was conducted to determine the fecal shedding rates of hev in various age groups of pigs and identify the genotypes of swine hev prevailing in korea. a total of 565 fecal samples were collected from suckling piglets, post-weaning pigs, growing pigs, and sows at 12 swine farms. rt-pcr was used to detect the prese ... | 2008 | 19122408 |
| molecular evidence of patient-to-patient transmission of hepatitis e virus in a hematology ward. | 2009 | 19128164 | |
| acute pancreatitis--complicating hepatitis e virus infection in a 7-year-old boy with glucose 6 phosphate dehydrogenase deficiency. | the association of viral hepatitis and acute pancreatitis is well described in the literature. most of the cases occur in conjunction with fulminant hepatitis a and hepatitis b virus infections. the recent literature reports increasing number of cases of this complication secondary to hepatitis e virus (hev) infection, mostly in young adults in regions endemic for the virus. till date, to the authors' knowledge, there are 14 well-documented cases of hev-associated acute pancreatitis in the liter ... | 2009 | 19129425 |
| direct identification of human enterovirus serotypes in cerebrospinal fluid by amplification and sequencing of the vp1 region. | human enteroviruses (hev) are a major cause of meningitis and other neurological disease. identification of hev serotypes in clinical cases is important for monitoring emergence of more pathogenic variants, epidemiological surveillance and investigating sources of infection. serotype identification is currently problematic following the widespread adoption of polymerase chain reaction (pcr)-based methods for hev detection in place of virus culture. | 2009 | 19135410 |
| effect of peptide pools on effector functions of antigen-specific cd8+ t cells. | peptide pools are routinely used to study antigen specific t cell responses, both in epitope discovery as well as immune monitoring. however, optimal assay conditions such as concentration of peptides or the best possible number of peptides per pool have not been defined. thus, we examined whether different peptide concentrations or varying number of peptides per pool influence effector functions of antigen-specific human t-cells. pbmc isolated from hla-a2-positive individuals with known respons ... | 2009 | 19135447 |
| evidence of widespread infection of avian hepatitis e virus (avian hev) in chickens from spain. | in the present work, 262 serum samples and 29 faeces pools from chickens coming from 29 healthy flocks were analysed by rt-pcr for detection of avian hev and by elisa using an ahev derived antigen for detection of anti-hev igg. additionally, other 300 randomly selected serum samples were also analysed by rt-pcr. seven serum samples were positive to rna detection. sequence analysis of both the helicase and the capsid genes revealed that the spanish isolates were clustered together and close relat ... | 2009 | 19136224 |
| [a man who contracted hepatitis e in the netherlands]. | a 55-year-old man was admitted to our hospital because of malaise, jaundice en cholestatic liver function impairment, 4 days after his return from vacation in surinam. serological tests were positive for igg and igm antibodies to hepatitis e virus (hev) and serum pcr was positive, consistent with hev infection. the infection was acquired in the netherlands and not abroad, considering the incubation period. the patient recovered spontaneously. hev infection is rare in the netherlands and is assoc ... | 2008 | 19137970 |
| determination of the henipavirus phosphoprotein gene mrna editing frequencies and detection of the c, v and w proteins of nipah virus in virus-infected cells. | the henipaviruses, nipah virus (niv) and hendra virus (hev), are highly pathogenic zoonotic paramyxoviruses. like many other paramyxoviruses, henipaviruses employ a process of co-transcriptional mrna editing during transcription of the phosphoprotein (p) gene to generate additional mrnas encoding the v and w proteins. the c protein is translated from the p mrna, but in an alternate reading frame. sequence analysis of multiple, cloned mrnas showed that the mrna editing frequencies of the p genes ... | 2009 | 19141449 |
| construction of an infectious cdna clone of hepatitis e virus strain je03-1760f that can propagate efficiently in cultured cells. | a full-length infectious cdna clone (pje03-1760f/wt) of a genotype 3 hepatitis e virus (hev) (strain je03-1760f) obtained from a faecal specimen was constructed in this study. upon transfection of the capped in vitro transcripts of pje03-1760f/wt into plc/prf/5 cells, the viral rna levels in the culture supernatant started to increase on day 6 post-transfection (p.t.) and reached 10(7) copies ml(-1) on day 28 p.t. detection of increasing numbers of cells with orf2 protein expression by immunoflu ... | 2009 | 19141456 |
| reduced prevalence of genotype 3 hev in shanghai pig farms and hypothetical homeostasis of porcine hev reservoir. | a total of 493 fecal samples collected from local shanghai pig farms were examined for hepatitis e virus (hev) after the introduction of stricter sanitary measures following outbreaks of a high fever-associated pig disease during 2006 and 2007. our investigation revealed that, while the overall occurrence of hev rna positives decreased by only 3.7%, the incidence of hev genotype 4 increased from 9.8% to 20.6% whereas the incidence of hev genotype 3 decreased from 16.2% to 1.6%. as well as demons ... | 2009 | 19150181 |
| outbreaks of hepatitis a among children in western india. | hepatitis a in most developing countries is a sporadic childhood disease, but lately focal outbreaks have been observed among children in india. during 2004, we investigated a large-scale outbreak of hepatitis among children living in a residential colony in daund taluka of district pune in the western region of india. in total, 123 overt and 56 sub-clinical cases were detected. all the patients were reactive for igm antibodies against hepatitis a virus (igm anti-hav) and were negative for igm a ... | 2009 | 19155033 |
| hepatitis e virus as an emerging cause of chronic liver disease in organ transplant recipients. | 2009 | 19157619 | |
| randomized-controlled phase ii clinical trial of a bacterially expressed recombinant hepatitis e vaccine. | the candidate recombinant hepatitis e vaccine, hev 239, protect monkeys against infection by hepatitis e virus (hev). the safety and immunogenicity of the vaccine for humans was assessed in a randomized controlled phase ii clinical trial. the study was conducted in an endemic area of southern china and consisted of a dose scheduling, involving 457 adults and a dose escalation component involving 155 high school students. the results showed that the vaccine is safe and immunogenic for humans and ... | 2009 | 19168109 |
| [comparison of the reliability of two elisa kits for detecting igm antibody against hepatitis e virus]. | to study the reliability of two elisa kits for detecting igm antibody against hepatitis e virus (hev). | 2008 | 19175178 |
| comparative analysis of different taqman real-time rt-pcr assays for the detection of swine hepatitis e virus and integration of feline calicivirus as internal control. | the aim of this study was to compare the performance of four taqman rt-pcr assays with a commonly used nested rt-pcr and to include the feline calicivirus (fcv) as an internal control. | 2009 | 19187137 |
| hepatitis e epidemic with bimodal peak in a town of north india. | an epidemic of viral hepatitis occurred in mandi gobindgarh town of punjab in northern india during year 2005-06. an attempt was made to study the outbreak clinically, serologically, and etiologically. | 2008 | 19189818 |
| acute hepatitis e virus infection in an hiv-infected person in the united states. | 2009 | 19189925 | |
| the course of hepatitis e virus infection in pigs after contact-infection and intravenous inoculation. | worldwide, hepatitis e virus (hev) genotype 3 is observed in pigs and transmission to humans is implied. to be able to estimate public health risks from e.g. contact with pigs or consumption of pork products, the transmission routes and dynamics of infection should be identified. hence, the course of hev-infection in naturally infected pigs should be studied. | 2009 | 19193209 |
| prevalence of hepatitis e virus (hev) infection in wild boars (sus scrofa leucomystax) and pigs in gunma prefecture, japan. | the prevalence of hepatitis e virus (hev) infection in wild boars and pigs in gunma prefecture, japan, was serologically and genetically examined. the positive detection rates of anti-hev igg and hev rna in the wild boars were 4.5% (4/89) and 1.1% (1/89), whereas those in the pigs were 74.6% (126/169) and 1.8% (3/169), respectively. the positive rates of anti-hev igg and hev rna on the 17 pig farms in the present study ranged from 20% to 100%, respectively. one male wild boar approximately 5 yea ... | 2009 | 19194072 |
| [epidemiology and management of hepatitis e in japan]. | 2009 | 19194091 | |
| [hepatitis e vaccine]. | 2009 | 19194092 | |
| anti-hev antibodies in domestic animal species and rodents from spain using a genotype 3-based elisa. | a truncated orf2 capsid hev antigen derived from a genotype 3 strain was developed in insect cells and insect larvae, and compared with the sar55 antigen and a commercial elisa. the antigen expressed in insect cells showed a better correlation with sar55 (kappa value (k)=0.84) than the insect larvae antigen (k=0.69), and a better reproducibility as indicated by the intra and interplate variation coefficients. commercial elisa designed for human diagnosis but adapted to animal use using specific ... | 2009 | 19200664 |
| henoch-schönlein purpura triggered by acute hepatitis e virus infection. | 2010 | 19201130 | |
| childhood autoimmune hemolytic anemia following hepatitis e virus infection. | 2009 | 19208072 | |
| hepatitis e virus is a leading cause of acute-on-chronic liver disease: experience from a tertiary centre in bangladesh. | acute-on-chronic liver failure (aclf) is common in bangladesh. acute viral e hepatitis is sporadically encountered in this country each year, with a rising incidence in the rainy season. this study aimed to identify the etiology of aclf in bangladesh. | 2009 | 19208515 |
| molecular biology and pathogenesis of hepatitis e virus. | the hepatitis e virus (hev) is a small rna virus and the etiological agent for hepatitis e, a form of acute viral hepatitis. the virus has a feco-oral transmission cycle and is transmitted through environmental contamination, mainly through drinking water. recent studies on the isolation of hev-like viruses from animal species also suggest zoonotic transfer of the virus. the absence of small animal models of infection and efficient cell culture systems has precluded virological studies on the re ... | 2008 | 19208971 |
| the expression, purification, and immunogenicity of a new chimeric virus-like particle. | virus-like particles (vlps) are highly immunogenic. in this study, gene fragments encoding hepatitis a virus (hav) vp1 (aa 24-171), a hepatitis e virus (hev) orf2 gene fragment encoding aa 431-615, and gene fragments encoding a nine-peptide linker were spliced together. the spliced gene fragments were then inserted into the expression vector pbv220, resulting in the recombinant plasmid pbv-ea342, which expressed a 342-amino acid fusion protein. the fusion protein was expressed in escherichia col ... | 2009 | 19210228 |
| metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis. | we analyzed viral nucleic acids in stool samples collected from 35 south asian children with nonpolio acute flaccid paralysis (afp). sequence-independent reverse transcription and pcr amplification of capsid-protected, nuclease-resistant viral nucleic acids were followed by dna sequencing and sequence similarity searches. limited sanger sequencing (35 to 240 subclones per sample) identified an average of 1.4 distinct eukaryotic viruses per sample, while pyrosequencing yielded 2.6 viruses per sam ... | 2009 | 19211756 |
| relationships among viral diagnostic markers and markers of liver function in acute hepatitis e. | diagnosis of acute hepatitis e has been based in many clinics predominantly on detection of anti-hev (hepatitis e virus) antibody. now, new assays have been developed to detect other hev markers. our aim was to investigate the relationships among hev diagnostic markers and liver function markers in acute hepatitis e. | 2009 | 19214676 |
| serological evidence of infection with hepatitis e virus among wild yezo-deer, cervus nippon yesoensis, in hokkaido, japan. | in this study, 520 serum samples from yezo-deer in the hidaka district, hokkaido, japan were examined by enzyme-linked immunosorbent assay to investigate whether the animals were infected with hepatitis e virus (hev). the distribution of optical density values showed a bimodal pattern and 181 samples (34.8%) were deemed to be antibody-positive against hev. at least five (2.8%) of the positive sera gave specific bands by western blot analysis. an age-dependent increase in prevalence of the antibo ... | 2009 | 19215576 |
| potent human monoclonal antibodies against sars cov, nipah and hendra viruses. | background: recently, several potently neutralizing fully human monoclonal antibodies (hmabs) targeting the severe acute respiratory syndrome-associated coronavirus (sars cov) s glycoprotein, and the g glycoprotein of the paramyxoviruses hendra virus (hev) and nipah virus (niv) have been discovered [corrected]. objective: to examine, compare and contrast the functional characteristics of hmabs with the potential for prophylaxis and treatment of diseases caused by sars cov, hev and niv. methods: ... | 2009 | 19216624 |
| indigenous hepatitis e virus infection in england: more common than it seems. | indigenous hepatitis e virus (hev) is increasingly diagnosed in england due to a better awareness and understanding of the virus. however, the true burden of infection and therefore its implication to public health remains undefined. | 2009 | 19217345 |
| characterization and zoonotic potential of endemic hepatitis e virus (hev) strains in humans and animals in hungary. | hepatitis e virus (hev) is a common cause of acute, fecally transmitted hepatitis in developing countries. identification of hev in indigenous human infection and in domestic pig raising the possibility that hev infection is also a zoonosis. objectives/study design: molecular detection and epidemiology of hev in humans (south-east hungary) with acute hepatitis and in domestic (pig, cattle) and wild (boar and roe-deer) animals (countrywide) by elisa and rt-pcr. | 2009 | 19217346 |
| isolation and characterization of a genotype 4 hepatitis e virus strain from an infant in china. | in the present study, a genotype 4 hev strain was identified in the fecal specimen from a seven months old infant with no symptom of hepatitis in shanghai children's hospital. the full capsid protein gene (orf2) sequence of this strain was determined by rt-pcr method. sequence analysis based on the full orf2 sequence indicated that this hev strain shared the highest sequence identity (97.6%) with another human hev strain isolated from a japanese patient who was infected by genotype 4 hev during ... | 2009 | 19220877 |
| clinical course and duration of viremia in vertically transmitted hepatitis e virus (hev) infection in babies born to hev-infected mothers. | infection with the hepatitis e virus (hev) causes a self-limiting acute hepatitis. however, prolonged viremia and chronic hepatitis has been reported in organ transplant recipients. vertically transmitted hev infection is known to cause acute hepatitis in newborn babies. the clinical course and duration of viremia in vertically transmitted hev infection in neonates in not known. we studied 19 babies born to hev infected mothers. babies were studied at birth and on a monthly basis to evaluate cli ... | 2009 | 19228284 |
| [molecular epidemiology of hepatitis e virus in hungary: endemic, food-borne zoonosis]. | hepatitis e virus (hev) is one of a common cause of acute, fecally transmitted hepatitis in developing countries. identification of hev in indigenous human infection and in domestic pig raises the possibility that hev infection is also a zoonosis. | 2009 | 19228570 |
| prevalence of antibody to hepatitis e virus among pet dogs in the jiang-zhe area of china. | in order to investigate the prevalence of hev infection and immunity in pet dogs in the jiang-zhe area of china, we examined 192 dog serum samples collected from 11 animal hospitals in this area for anti-hev igg and hev rna, using elisa and rt-pcr assays, respectively. two dogs were injected intravenously with swine hev to obtain anti-hev antibody positive serum for elisa assay. after the elisa assay, 26 samples showing od values higher than 0.20 were selected and examined by dot-blot assay, and ... | 2009 | 19229761 |
| full-length sequence of genotype 3 hepatitis e virus derived from a pig in thailand. | hepatitis e virus (hev) infection in pigs was investigated in two principal swine farming areas in thailand. anti-hev antibodies and hev rna in sera were examined in 258 pigs reared on five commercial farms from age 1 to 6.5 months and sows. overall, 167 of 258 (64.7%) pigs were positive for anti-hev igg, while 20 of 258 (7.75%) had detectable hev rna. sequence analysis of 20 hev isolates obtained from viremic pigs revealed that they were 92.3-100% identical to each other and had 82.2-88.2% nucl ... | 2009 | 19235846 |
| sources of hepatitis e virus genotype 3 in the netherlands. | non-travel-related hepatitis e virus (hev) genotype 3 infections in persons in the netherlands may have a zoonotic, foodborne, or water-borne origin. possible reservoirs for hev transmission by water, food, and animals were studied. hev genotype 3/open reading frame 2 sequences were detected in 53% of pig farms, 4% of wild boar feces, and 17% of surface water samples. hev sequences grouped within 4 genotype 3 clusters, of which 1 is so far unique to the netherlands. the 2 largest clusters contai ... | 2009 | 19239749 |
| hepatitis e virus antibodies in patients with chronic liver disease. | in the united states, the seroprevalence rate for hepatitis e virus (hev) is approximately 20%. this study examined hev seroprevalence in persons with and without chronic liver disease. our data indicate that hev seropositivity is high in patients with chronic liver disease and that hev seroprevalence increases significantly with age. | 2009 | 19239770 |
| detection and molecular characterization of swine hepatitis e virus in north carolina swine herds and their faecal wastes. | recent findings of almost genetically indistinguishable swine and human strains, have suggested swine play a role in the transmission of hepatitis e virus (hev). the extent to which hev may be present and persist in the faecal waste generated from intensive swine operations is largely unknown. the fate of swine waste liquid is often land application, possibly resulting in unintentional seepage into groundwater or run-off into surface waters, hence validating concerns of human exposure risks. fre ... | 2009 | 19240360 |
| immunogenicity and protective efficacy in rhesus monkeys of a recombinant orf2 protein from hepatitis e virus genotype 4. | several antigens derived from hepatitis e virus (hev) genotype 1 strains have shown immunogenicity and efficacy against hepatitis e in primates and humans. however, the protective effect of a vaccine derived from hev genotype 4 has not been studied. this study aimed to evaluate the immunogenicity and protective efficacy of the t1-orf2 (56 kda) capsid protein derived from hev strain t1 (genotype 4) in rhesus monkeys. two doses (40 microg) of alum-absorbed t1-orf2 capsid protein were administered ... | 2009 | 19240977 |
| pseudotumor cerebri in childhood hepatitis e virus infection. | 2009 | 19245388 | |
| hev infection as an aetiologic factor for acute hepatitis: experience from a tertiary hospital in bangladesh. | acute hepatitis is seen sporadically round the year in bangladesh. the incidence of acute viral hepatitis e increases after floods as this allows sewerage contamination of piped and groundwater. the aim of this retrospective study was to assess the burden of hepatitis e virus (hev infection) in bangladesh. patients attending the hepatology unit iii of the bangabandhu sheikh mujib medical university, during june 2004-december 2006, were included in the study. all viral markers were tested by enzy ... | 2009 | 19248644 |
| epidemiology of hepatitis e in northeastern china, south korea and japan. | the seroprevalence of hepatitis e virus (hev) infection in northeastern asia is unknown. this study was conducted to gain insight into the epidemiology of hev that has been obscure in northeastern china, south korea and japan. | 2009 | 19249101 |
| hepatitis e: a common cause of acute viral hepatitis. | to investigate the etiology of acute hepatitis in adult patients admitted in a hospital. | 2009 | 19260572 |
| hepatitis e virus infection in a 7-year-old boy with glucose 6-phosphate dehydrogenase deficiency. | 2009 | 19262255 | |
| sequence analysis and comparison of avian hepatitis e viruses from australia and europe indicate the existence of different genotypes. | avian hepevirus infections were detected in chickens suffering from big liver and spleen disease or hepatitis-splenomegaly syndrome in australia, the usa and europe. available data indicate their genetic relationship to mammalian hepatitis e virus (hev). in the present study, the near-complete genomic sequences of an australian and a european isolate of avian hepatitis e virus (avian hev) are reported for the first time. furthermore, the phylogenetic relationship to other avian hevs is determine ... | 2009 | 19264623 |
| orf3 protein of hepatitis e virus interacts with the bbeta chain of fibrinogen resulting in decreased fibrinogen secretion from huh-7 cells. | the orf3 protein of hepatitis e virus (hev), the precise cellular functions of which remain obscure, was used in a yeast two-hybrid screen to identify its cellular binding partners. one of the identified interacting partners was fibrinogen bbeta protein. the orf3-fibrinogen bbeta interaction was verified by co-immunoprecipitation and fluorescence resonance energy transfer in mammalian cells. fibrinogen is a hepatic acute-phase protein and serves as a central molecule that maintains host homeosta ... | 2009 | 19264644 |
| open reading frame structure analysis as a novel genotyping tool for hepatitis e virus and the subsequent discovery of an inter-genotype recombinant. | accurate viral genotyping is important. here i investigate genotypes in hepatitis e virus (hev) and find that the open reading frame (orf) structure (the lengths of three orfs and the overlapping relationships among the orfs) can be a good criterion for genotyping hev. an inter-genotype recombinant (genbank accession no. dq450072) was revealed by analysing the orf structure and confirmed by phylogenetic analyses. this discovery of the inter-genotype recombinant indicates that genotyping in hev s ... | 2009 | 19264661 |
| simulating henipavirus multicycle replication in a screening assay leads to identification of a promising candidate for therapy. | nipah (niv) and hendra (hev) viruses are emerging zoonotic paramyxoviruses that cause encephalitis in humans, with fatality rates of up to 75%. we designed a new high-throughput screening (hts) assay for inhibitors of infection based on envelope glycoprotein pseudotypes. the assay simulates multicycle replication and thus identifies inhibitors that target several stages of the viral life cycle, but it still can be carried out under biosafety level 2 (bsl-2) conditions. these features permit a sc ... | 2009 | 19264786 |
| clinical features and predictors of outcome in acute hepatitis a and hepatitis e virus hepatitis on cirrhosis. | acute hepatitis a and e are recognized triggers of hepatic decompensation in patients with cirrhosis, particularly from the indian subcontinent. however, the resulting acute-on-chronic liver failure (aclf) has not been well characterized and no large studies are available. our study aimed to evaluate the clinical profile and predictors of 3-month mortality in patients with this distinctive form of liver failure. | 2009 | 19267864 |
| sporadic acute hepatitis e occurred constantly during the last decade in northeast japan. | recent studies have shown that indigenous hepatitis e virus (hev) strains cause hepatitis e in industrialized countries. we aimed to clarify the characteristics of hev infection in sporadic hepatitis patients during the last decade in miyagi, northeast japan. | 2009 | 19271116 |
| role of acute viral hepatitis as a confounding factor in antituberculosis treatment induced hepatotoxicity. | drug induced hepatotoxicity (dih) is an important and commonly encountered adverse effect with antituberculosis (anti-tb) treatment. acute viral hepatitis (avh) is an important confounding reason which clinically, biochemically and histologically mimics dih. | 2009 | 19287059 |