Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| estrogenic activity of styrene oligomers after metabolic activation by rat liver microsomes. | in this study we examined estrogenic activity of styrene oligomers after metabolic activation by rat liver microsomes. trans-1,2-diphenylcyclobutane (tcb), cis-1,2-diphenylcyclobutane (ccb), 1,3-diphenylpropane, 2,4-diphenyl-1-butene, 2,4,6-triphenyl-1-hexene, and 1-alpha-phenyl-4ss-(1 -phenylethyl)tetralin were negative in the yeast estrogen screening assay and estrogen reporter assay using estrogen-responsive human breast cancer cell line mcf-7. however, tcb exhibited estrogenic activity after ... | 2003 | 12611662 |
| nuclear localization of senescence marker protein-30, smp30, in cultured mouse hepatocytes and its similarity to rna polymerase. | senescence marker protein-30 (smp30), expressed mostly in the liver, protects cells against various injuries by stimulating membrane calcium-pump activity. by immunohistochemistry and western blotting, we found that smp30 was in both the nuclei and cytoplasm of cultured mouse hepatocytes. by a homology search, we found that a domain of the smp30 sequence 51 amino acid residues long was 60-66% similar to bacterial and yeast rna polymerases. | 2003 | 12619687 |
| characterization of carbohydrate recognition by langerin, a c-type lectin of langerhans cells. | langerin is a type ii transmembrane cell surface receptor found on langerhans cells. the extracellular domain of langerin consists of a neck region containing a series of heptad repeats and a c-terminal c-type carbohydrate-recognition domain (crd). a role for langerin in processing of glycoprotein antigens has been proposed, but until now there has been little study of the langerin protein. in this study, analytical ultracentrifugation and circular dichroism spectroscopy of recombinant soluble f ... | 2003 | 12626394 |
| the analgesic, antipyretic and anti-inflammatory activity of diospyros variegata kruz. | pharmacological studies were conducted with the hexane extract of the dry stem of diospyros variegata kruz. (ebenaceae) on experimental animals for evaluating the analgesic, antipyretic and anti-inflammatory activities. in the analgesic test, the hexane extract elicited inhibitory intensity on acetic acid-induced writhing response and on the late phase of formalin test but possessed only a weak effect on the tail-flick response and on the early phase of formalin test. the hexane extract also eli ... | 2003 | 12639744 |
| proteasomal interactors control activities as diverse as the cell cycle and glutaminergic neurotransmission. | the six regulatory non-redundant atpases in the base of the 19 s regulator of the 26 s proteasome belong to the aaa superfamily of atpases. yeast two-hybrid genetic screens, biochemical analyses and cell biological studies have identified and characterized new interactors of the human s6 (rpt3) and s8 (rpt6) atpases of the 19 s regulator of the 26 s proteasome. the s6 atpase interacts with gankyrin. this protein is found in purified human 26 s proteasomes and in a smaller complex(es) containing ... | 2003 | 12653665 |
| glycopeptide specificity of the secretory protein folding sensor udp-glucose glycoprotein:glucosyltransferase. | secretory and membrane n-linked glycoproteins undergo folding and oligomeric assembly in the endoplasmic reticulum with the aid of a folding mechanism known as the calnexin cycle. udp-glucose glycoprotein:glucosyltransferase (uggt) is the sensor component of the calnexin cycle, which recognizes these glycoproteins when they are incompletely folded, and transfers a glucose residue from udp-glucose to n-linked man9-glcnac2 glycans. to determine how uggt recognizes incompletely folded glycoproteins ... | 2003 | 12671684 |
| modifications of glyceraldehyde-3-phosphate dehydrogenase induced by increasing concentrations of peroxynitrite: early recognition by 20s proteasome. | peroxynitrite, a potent oxidizing and nitrating species, induces covalent modifications of biomolecules in a number of pathological conditions. in previous studies with s. cerevisiae, glyceraldehyde-3-phosphate dehydrogenase (gapdh) was identified as being especially susceptible to nitration by peroxynitrite. the activity of this enzyme was strongly inhibited by low doses of peroxynitrite in yeast and in cultured rat astrocytes. here, the sequence of modifications of isolated mammalian gapdh ind ... | 2003 | 12675516 |
| endocytosis of the at1a angiotensin receptor is independent of ubiquitylation of its cytoplasmic serine/threonine-rich region. | agonist-induced internalisation of the rat type 1a (at(1a)) angiotensin ii receptor is associated with phosphorylation of a serine/threonine-rich region in its cytoplasmic tail. in yeast, hyperphosphorylation of the alpha-factor pheromone receptor regulates endocytosis of the receptor by facilitating the monoubiquitylation of its cytoplasmic tail on lysine residues. the role of receptor ubiquitylation in at(1a) receptor internalisation was evaluated by deletion or replacement of lysine residues ... | 2003 | 12676183 |
| vascular smooth muscle polyploidization--from mitotic checkpoints to hypertension. | aging and hypertension are accompanied by an increase in mass and rigidity of arterial walls. at capacitance arteries, the enlargement and stiffness of the medial smooth muscle layer promote systolic hypertension and contribute to left ventricular hypertrophy and cardiovascular morbidity. morphological studies have demonstrated that vascular smooth muscle cell (vsmc) hypertrophy, with minimal hyperplasia, causes the enlargement of vascular smooth muscle at capacitance arteries, and that vsmc hyp ... | 2003 | 12695657 |
| regulation of the bone-specific osteocalcin gene by p300 requires runx2/cbfa1 and the vitamin d3 receptor but not p300 intrinsic histone acetyltransferase activity. | p300 is a multifunctional transcriptional coactivator that serves as an adapter for several transcription factors including nuclear steroid hormone receptors. p300 possesses an intrinsic histone acetyltransferase (hat) activity that may be critical for promoting steroid-dependent transcriptional activation. in osteoblastic cells, transcription of the bone-specific osteocalcin (oc) gene is principally regulated by the runx2/cbfa1 transcription factor and is stimulated in response to vitamin d(3) ... | 2003 | 12697832 |
| genetic interaction between a chaperone of small nucleolar ribonucleoprotein particles and cytosolic serine hydroxymethyltransferase. | srp40p is a nonessential yeast nucleolar protein proposed to function as a chaperone for over 100 small nucleolar ribonucleoprotein particles that are required for rrna maturation. to verify and expand on its function, genetic screens were performed for the identification of genes that were lethal when mutated in a srp40 null background (srp40delta). unexpectedly, mutation of both cytosolic serine hydroxymethyltransferase (shm2) and one-carbon tetrahydrofolate synthase (ade3) was required to ach ... | 2003 | 12700234 |
| anti-inflammatory effect and low ulcerogenic activity of etodolac, a cyclooxygenase-2 selective non-steroidal anti-inflammatory drug, on adjuvant-induced arthritis in rats. | adjuvant arthritic rats are known to be more susceptible to gastric damage induced by non-steroidal anti-inflammatory drugs (nsaids) than are normal rats. we compared the relative gastric safety profile of etodolac with those of meloxicam, diclofenac sodium and indometacin in adjuvant arthritic rats and normal rats or mice. as a measure of the safety profiles of nsaids, we used the safety index, the ratio of the dose that elicits gastric mucosal lesions to the effective dose as an anti-inflammat ... | 2003 | 12711837 |
| [selenoproteins in rats with chronic selenium intoxication]. | weaning male wistar rats were fed with a torula-yeast based semisynthetic diet supplemented with na2seo3 to provide selenium (se) 0.2 or 0.5 mg/kg (adequate se or high sediet) respectively for 20 weeks. by the end of experiment, rats were sacrificed and various tissue of rats were collected to determine the activities of se-containing enzymes and the mrnas level of selenoprotein p and selenoprotein w and se concentration. livers were examined for pathological changes. it was found that the gain ... | 1999 | 12712720 |
| ca2+-dependent phosphorylation of syntaxin-1a by the death-associated protein (dap) kinase regulates its interaction with munc18. | syntaxin-1 is a key component of the synaptic vesicle docking/fusion machinery that binds with vamp/synaptobrevin and snap-25 to form the snare complex. modulation of syntaxin binding properties by protein kinases could be critical to control of neurotransmitter release. using yeast two-hybrid selection with syntaxin-1a as bait, we have isolated a cdna encoding the c-terminal domain of death-associated protein (dap) kinase, a calcium/calmodulin-dependent serine/threonine protein kinase. expressi ... | 2003 | 12730201 |
| post-translational modifications of three members of the human map1lc3 family and detection of a novel type of modification for map1lc3b. | the molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. recently, both yeast apg8p and its rat homologue map1lc3 were identified as essential constituents of autophagosome membrane as a processed form. in addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical c-t ... | 2003 | 12740394 |
| interaction of the deafness-dystonia protein ddp/timm8a with the signal transduction adaptor molecule stam1. | the mohr-tranebjaerg-jensen deafness-dystonia-optic atrophy protein ddp/timm8a is translated on cytoplasmic ribosomes but targeted ultimately to the mitochondrial intermembrane space, where it is involved in mitochondrial protein import. stam1 is a cytoplasmic signal-transducing adaptor molecule implicated in cytokine signaling. we report here a direct interaction between ddp and stam1, identified by yeast two-hybrid screening and confirmed by co-immunoprecipitation, fusion protein "pull downs," ... | 2003 | 12745081 |
| double-color fluorescence in situ hybridization with rna probes. | 2003 | 12765014 | |
| overexpression of a small medicinal peptide from ginseng in the yeast pichia pastoris. | a medicinal peptide, gsp, which was initially extracted from the traditional medicinal herb ginseng, has potential use as a drug against diabetes. gsp is a low molecular weight protein that we have secreted in a recombinant form from the yeast pichia pastoris. a dna fragment encoding four copies of the gsp protein each separated by a basic amino acid was synthesized and inserted into the p. pastoris expression vector plasmid ppic9. after electroporation of the resulting vector, ppic9-gsp, into t ... | 2003 | 12767805 |
| the tyrosine kinase pyk2 regulates arf1 activity by phosphorylation and inhibition of the arf-gtpase-activating protein asap1. | proline-rich tyrosine kinase 2 (pyk2), a non-receptor tyrosine kinase structurally related to focal adhesion kinase, has been implicated in the regulation of mitogen-activated protein kinase cascades and ion channels, the induction of apoptosis, and in the modulation of the cytoskeleton. in order to understand how pyk2 signaling mediates these diverse cellular functions, we performed a yeast two-hybrid screening using the c-terminal part of pyk2 that contains potential protein-protein interactio ... | 2003 | 12771146 |
| arp-1/coup-tf ii determines hepatoma phenotype by acting as both a transcriptional repressor of microsomal triglyceride transfer protein and an inducer of cyp7a1. | l35 and fao cells were derived as single cell isolates from h35 cells. whereas l35 cells do not express microsomal triglyceride transfer protein (mtp), which regulates lipoprotein secretion, they express cyp7a1, which regulates bile acid synthesis from cholesterol. fao cells display the opposite phenotype (i.e. expression of mtp but not cyp7a1). we examined the molecular basis of the transcriptional inactivation of the mtp gene in l35 cells. nested deletion and mutagenesis studies show that a co ... | 2003 | 12777384 |
| evaluation of antipyretic potential of cleome viscosa linn. (capparidaceae) extract in rats. | the antipyretic activity of a methanol extract of cleome viscosa linn. (cvme) was investigated for its potential on normal body temperature and yeast-induced pyrexia in albino rats. the cvme, at doses of 200, 300, and 400mg/kg bw p.o., showed significant reduction in normal body temperature and yeast-provoked elevated temperature in a dose-dependent manner. the effect also extended upto 5h after the drug administration. the anti-pyretic effect of cvme was comparable to that of paracetamol (150mg ... | 2003 | 12787948 |
| selectivity of 4,5,6,7-tetrabromobenzimidazole as an atp-competitive potent inhibitor of protein kinase ck2 from various sources. | like the previously reported 4,5,6,7-tetrabromobenzotriazole (tbbt), the structurally related 4,5,6,7-tetrabromobenzimidazole (tbbz) is a selective atp-competitive inhibitor of protein kinase ck2 from such divergent sources as yeast, rat liver, neurospora crassa and candida tropicalis, with k(i) values in the range 0.5-1 microm. it is virtually inactive vs. pka, pkc, and a very weak inhibitor of protein kinase ck1. the corresponding tetrachlorobenzimidazole (tcbz) is a much weaker inhibitor of c ... | 2003 | 12788077 |
| identification of the motifs and amino acids in aggrecan g1 and g2 domains involved in product secretion. | members of the large aggregating chondroitin sulfate proteoglycans are characterized by an n-terminal fragment known as g1 domain, which is composed of an immunoglobulin (igg)-like motif and two tandem repeats (tr). previous studies have indicated that the expressed product of aggrecan g1 domain was not secreted. here we demonstrated that the inability of g1 secretion was associated with the tandem repeats but not the igg-like motif, and specifically with tr1 of aggrecan. we also demonstrated th ... | 2003 | 12795619 |
| insulin induces the expression of the sharp-2/stra13/dec1 gene via a phosphoinositide 3-kinase pathway. | transcription of the rat fatty acid synthase (fas) gene in the rat liver can be regulated by feeding a high carbohydrate diet. a carbohydrate response element (chore) located on the rat fas gene promoter has been identified. using multiple copies of the chore as the bait in a yeast one-hybrid system, a rat liver cdna library was screened, and the cdna of chore-binding proteins was cloned. a positive clone that encodes a basic helix-loop-helix protein, enhancer of split- and hairy-related protein ... | 2003 | 12796501 |
| mutant huntingtin increases nuclear corepressor function and enhances ligand-dependent nuclear hormone receptor activation. | there is increasing evidence that transcriptional dysregulation is important in huntington's disease pathogenesis. the transcriptional protein, nuclear corepressor (ncor), acts to repress transcription of nuclear hormone receptors, such as the thyroid hormone receptor (tr) and retinoic acid receptor, in the absence of their appropriate ligand. ncor has been shown to bind to the mutated huntingtin protein in a yeast two-hybrid screen. this aberrant interaction may have profound effects on both th ... | 2003 | 12799135 |
| kinase pathway database: an integrated protein-kinase and nlp-based protein-interaction resource. | protein kinases play a crucial role in the regulation of cellular functions. various kinds of information about these molecules are important for understanding signaling pathways and organism characteristics. we have developed the kinase pathway database, an integrated database involving major completely sequenced eukaryotes. it contains the classification of protein kinases and their functional conservation, ortholog tables among species, protein-protein, protein-gene, and protein-compound inte ... | 2003 | 12799355 |
| activation of the tumour suppressor kinase lkb1 by the ste20-like pseudokinase strad. | the lkb1 gene encodes a serine/threonine kinase mutated in peutz-jeghers cancer syndrome. despite several proposed models for lkb1 function in development and in tumour suppression, the detailed molecular action of lkb1 remains undefined. here, we report the identification and characterization of an lkb1-specific adaptor protein and substrate, strad (ste20 related adaptor). strad consists of a ste20- like kinase domain, but lacks several residues that are indispensable for intrinsic catalytic ac ... | 2003 | 12805220 |
| phosphoinositide profiling in complex lipid mixtures using electrospray ionization mass spectrometry. | phosphoinositides (phosphorylated derivatives of phosphatidylinositol, pi) are versatile intracellular signaling lipids whose occurrence in low concentrations complicates direct mass measurements. here we present a sensitive method to detect, identify and quantify phosphatidylinositol phosphate (pip) and phosphatidylinositol bisphosphate (pip(2)) with different fatty acid compositions (phosphoinositide profiles) in total lipid extracts by electrospray ionization mass spectrometry (esi-ms). using ... | 2003 | 12808461 |
| ini, a small nuclear protein that enhances the response of the connexin43 gene to estrogen. | this article describes the structural and functional characterization of ini (af495522), a novel highly conserved zinc-finger protein that had been identified by screening an estrogen-induced rat myometrial expression library. ini localizes to the nucleus of hela cells and binds to the proximal connexin43 (cx43) promoter, as demonstrated by emsa. in addition, transient transfection experiments performed with estrogen receptor alpha (eralpha) cdna show that overexpression of ini enhances, in a do ... | 2003 | 12810571 |
| sialylglycoconjugates and sialyltransferase activity in the fungus cryptococcus neoformans. | cryptococcus neoformans is a fungal pathogen associated with systemic mycoses in up to 10% of aids patients. c. neoformans yeasts express sialic acids on the cell wall, where they play an anti-phagocytic role, and may represent a virulence factor at the initial phase of infection. since the nature of the sialic acid-carrying components is undefined in c. neoformans, our aim in the present work was to identify sialylated molecules in this fungus and study the sialylation process. c. neoformans ye ... | 2002 | 12815227 |
| [mechanisms of degradation of the fungal ornithine decarboxylase]. | ornithine decarboxylase (odc) is the first enzyme in polyamine biosynthesis in numerous living organisms, from bacteria to mammalian cells. its control is under negative feedback regulation by the end products of the pathway. in dimorphic fungi, odc activity and therefore polyamine concentrations are related to the morphogenetic process. from the fission yeast schizosaccharomyces pombe to human, polyamines induce antizyme synthesis which in turn inactivates odc. this is hydrolyzed by the 26s pro ... | 2003 | 12825973 |
| influence of cytosolic ags3 on receptor--g protein coupling. | activator of g protein signaling 3 (ags3) activates the gbetagamma mating pathway in yeast in a manner that is independent of heptahelical receptors. it competes with gbetagamma subunits to bind gdp-bound gi/o(alpha) subunits via four repeated g protein regulatory (gpr) domains in the carboxyl-terminal half of the molecule. however, little is known about the functional role of ags3 in cellular signaling. here the effect of ags3 on receptor-g protein coupling was examined in an sf9 cell membrane- ... | 2003 | 12834360 |
| identification of novel erbb3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system. | analysis of membrane protein interactions is difficult because of the hydrophobic nature of these proteins, which often renders conventional biochemical and genetic assays fruitless. this is a substantial problem because proteins that are integral or associated with membranes represent approximately one-third of all proteins in a typical eukaryotic cell. we have shown previously that the modified split-ubiquitin system can be used as a genetic assay for the in vivo detection of interactions betw ... | 2003 | 12840049 |
| hsp90 inhibition accelerates cell lysis. anti-hsp90 ribozyme reveals a complex mechanism of hsp90 inhibitors involving both superoxide- and hsp90-dependent events. | the 90 kda heat shock protein, hsp90, is an abundant molecular chaperone participating in the cytoprotection of eukaryotic cells. here we analyzed the involvement of hsp90 in the maintenance of cellular integrity using partial cell lysis as a measure. inhibition of hsp90 by geldanamycin, radicicol, cisplatin, and novobiocin induced a significant acceleration of detergent- and hypotonic shock-induced cell lysis. the concentration and time dependence of cell lysis acceleration was in agreement wit ... | 2003 | 12842893 |
| efficient hsp90-independent in vitro activation by hsc70 and hsp40 of duck hepatitis b virus reverse transcriptase, an assumed hsp90 client protein. | hsp90 is a specialized chaperone that controls the activity of many key regulator proteins such as steroid hormone receptors (shrs). hormone binding, and therefore shr activation, requires hsp90, which is loaded onto the receptors by a series of events involving hsp70, hsp40, hop, and p23. the reverse transcriptase (rt) of hepatitis b viruses, small dna-containing viruses that replicate via an rna intermediate, has been reported to depend similarly on hsp90 for enzymatic activity. using an in vi ... | 2003 | 12851401 |
| preparation of recombinant rat eosinophil-associated ribonuclease-1 and -2 and analysis of their biological activities. | rat eosinophils contain eosinophil-associated ribonucleases (ears) in their granules. ears are thought to be synthesized as pre-forms and stored in the granules as mature forms. however, the n-terminal amino acid of mature ear-1 and ear-2 is still controversial. therefore, we prepared two recombinant mature forms of ear-1 and ear-2 in which the n-terminal amino acids are ser24 (s) [ear-1 (s) and ear-2 (s)] and gln26 (q) [ear-1 (q) and ear-2 (q)], and analyzed their biological activities by compa ... | 2003 | 12853122 |
| atrial natriuretic peptide induces natriuretic peptide receptor-cgmp-dependent protein kinase interaction. | circulating natriuretic peptides such as atrial natriuretic peptide (anp) counterbalance the effects of hypertension and inhibit cardiac hypertrophy by activating cgmp-dependent protein kinase (pkg). natriuretic peptide binding to type i receptors (npra and nprb) activates their intrinsic guanylyl cyclase activity, resulting in a rapid increase in cytosolic cgmp that subsequently activates pkg. phosphorylation of the receptor by an unknown serine/threonine kinase is required before ligand bindin ... | 2003 | 12855709 |
| glucuronidation and excretion of nonylphenol in perfused rat liver. | nonylphenol, an environmental estrogenic chemical, is reported to have adverse effects on the reproductive organs of animals. in this study, the metabolism of nonylphenol and that of other alkylphenols in the rat liver was investigated using liver perfusion. alkylphenols (nonylphenol, hexylphenol, butylphenol, and ethylphenol) were glucuronidated by rat liver microsomes. nonylphenol was found to be conjugated with glucuronic acid by an isoform of udp-glucuronosyltransferase, ugt2b1, expressed in ... | 2003 | 12867487 |
| inositol 1,4,5-trisphosphate receptor ubiquitination is mediated by mammalian ubc7, a component of the endoplasmic reticulum-associated degradation pathway, and is inhibited by chelation of intracellular zn2+. | in response to activation of certain cell surface receptors, inositol 1,4,5-trisphosphate receptors (insp3rs), which are located in the endoplasmic reticulum, can be rapidly ubiquitinated and then degraded by the proteasome. ubiquitination is mediated by the concerted action of ubiquitin-conjugating enzymes (ubcs or e2s) and ubiquitin-protein ligases (e3s). in the present study we have examined the enzymology of ubiquitination of endogenous insp3rs in muscarinic agonist-stimulated sh-sy5y human ... | 2003 | 12869571 |
| validation of the hsp150 polypeptide carrier and hsp150 promoter in expression of rat alpha2,3-sialyltransferase in yeasts. | heterologous glycoproteins usually do not fold properly in yeast cells and fail to leave the endoplasmic reticulum. here we show that the hsp150delta polypeptide carrier promoted proper folding and secretion of the catalytic ectodomain of rat alpha2,3-sialyltransferase (st3ne) in pichia pastoris. the efficiency of the hsp150delta carrier in p. pastoris and saccharomyces cerevisiae was at least as high as that of the mfalpha carrier. most of hsp150delta-st3ne and mfalpha-st3ne remained noncovalen ... | 2003 | 12892504 |
| alpha-cardiac actin (actc) binds to the band 3 (ae1) cardiac isoform. | the band 3 protein is the major integral protein present in the erythrocyte membrane. two tissue-specific isoforms are also expressed in kidney alpha intercalated cells and in cardiomyocytes. it has been suggested that the cardiac isoform predominantly mediates the anion exchange in cardiomyocytes, but the role of the cytoplasmic domain of the band 3 (cdb3) protein in the cardiac tissue is unknown. in order to characterize novel associations of the cdb3 in the cardiac tissue, we performed the tw ... | 2003 | 12898519 |
| [screening the trans-action factors binding to the enhancer element of rat gst-p by yeast one-hybrid system]. | to investigate the diversity of the mechanisms of rat glutathione s-transferase p (gst-p) gene expression regulation and the relation between rat gst-p gene expression and carcinogenesis. to search the trans-action factors binding to the enhancer element of rat gst-p (glutathione s-transferase p enhancer, gpe i). | 2000 | 12903441 |
| a porphobilinogen deaminase (pbgd) ran-binding protein interaction is implicated in nuclear trafficking of pbgd in differentiating glioma cells. | porphobilinogen deaminase (pbgd) is a rate-limiting enzyme of the heme biosynthesis pathway, whose level is elevated in various human tumors. pbgd was observed in both nuclear and cytoplasmic fractions of c6 glioma cells by immunostaining. during mitosis, chromatids were intensely stained for pbgd in comparison to the interphase chromatin. using the yeast two-hybrid system, we identified ranbpm, the nuclear ran-binding protein, as an interacting partner of pbgd. during butyrate-induced different ... | 2003 | 12917623 |
| an sh2 domain-dependent, phosphotyrosine-independent interaction between vav1 and the mer receptor tyrosine kinase: a mechanism for localizing guanine nucleotide-exchange factor action. | mer belongs to the mer/axl/tyro3 receptor tyrosine kinase family, which regulates immune homeostasis in part by triggering monocyte ingestion of apoptotic cells. mutations in mer can also cause retinitis pigmentosa, again due to defective phagocytosis of apoptotic material. although, some functional aspects of mer have been deciphered, how receptor activation lead to the physiological consequences is not understood. by using yeast two-hybrid assays, we identified the carboxyl-terminal region of ... | 2003 | 12920122 |
| induction of multidrug resistance protein 3 in rat liver is associated with altered vectorial excretion of acetaminophen metabolites. | treatment with the microsomal enzyme inducer trans-stilbene oxide (tso) can decrease biliary excretion of acetaminophen-glucuronide (aa-gluc) and increase efflux of aa-gluc into blood. the hepatic canalicular multidrug resistance protein (mrp) 2 and sinusoidal protein mrp3 transport aa-gluc conjugates into bile and blood, respectively. thus, tso-induced alterations in the vectorial excretion of aa-gluc may occur via increased hepatic mrp3 levels. the goal of this study was to determine whether t ... | 2003 | 12920174 |
| calorie restriction and aging: review of the literature and implications for studies in humans. | calorie restriction (cr) extends life span and retards age-related chronic diseases in a variety of species, including rats, mice, fish, flies, worms, and yeast. the mechanism or mechanisms through which this occurs are unclear. cr reduces metabolic rate and oxidative stress, improves insulin sensitivity, and alters neuroendocrine and sympathetic nervous system function in animals. whether prolonged cr increases life span (or improves biomarkers of aging) in humans is unknown. in experiments of ... | 2003 | 12936916 |
| deacetylase activity is required for camp activation of a subset of creb target genes. | many hormones activate transcription by raising the level of camp within cells. in one well studied pathway, camp induces protein kinase a to phosphorylate the transcription factor creb, which binds to a consensus sequence, the camp-regulated enhancer, found in many target genes. a generally accepted model suggests that phosphorylated creb recruits the histone acetyltransferase cbp to activate transcription. in contrast, histone deacetylases have been linked to the cessation of creb-dependent tr ... | 2003 | 12939274 |
| different isoforms of prip-interacting protein with methyltransferase domain/trimethylguanosine synthase localizes to the cytoplasm and nucleus. | a protein family including the recently identified pimt/tgs1 (prip-interacting protein with methyltransferase domain/trimethylguanosine synthase) was identified by searching databases for homologues of a newly identified drosophila protein with rna-binding activity and methyltransferase domain. antibodies raised against a short peptide of the mammalian homologue show a 90-kda isoform expressed specifically in rat brain and testis and a 55-kda form expressed ubiquitously. in hela cells, the large ... | 2003 | 12943661 |
| identification of eloa-bp1, a novel elongin a binding protein with an exonuclease homology domain. | the elongin complex stimulates the rate of transcription elongation by rna polymerase ii by suppressing the transient pausing of the polymerase at many sites along the dna template. elongin is composed of a transcriptionally active a subunit, and two positive regulatory b and c subunits. although the nh(2)-terminal approximately 120 amino acid region of elongin a is dispensable for its transcriptional activity in vitro, it shares significant sequence similarity with the nh(2)-terminus of other c ... | 2003 | 12943681 |
| inhibition of beta-fructofuranosidases and alpha-glucosidases by synthetic thio-fructofuranoside. | a synthetic beta-thio-fructofuranoside of mercaptoethanol inhibited not only beta-fructofuranosidases but also alpha-glucosidases. the compound was hardly hydrolyzed by the glycosidases. the thio-fructoside competitively inhibited beta-fructofuranosidases from aspergillus niger, candida sp., and saccharomyces cerevisiae, but not arthrobacter beta-fructofuranosidase at all. sucrase activity of rat intestinal sucrase/isomaltase complex was also suppressed in the presence of the thio-fructoside. th ... | 2003 | 12951505 |
| studies on the use of cassia singueana in malaria ethnopharmacy. | cassia singueana (family: fabaceae) is used in northern nigeria for the treatment of acute malaria attack. we investigated the activities of the methanol extract of the root bark of this plant against rodent plasmodia infection, nociception, pyrexia and inflammation in mice and rats. the studies were carried out using acetic acid-induced writhing, hot plate algesia, rodent plasmodia (plasmodium berghei) in mice; formalin test, yeast-induced pyrexia and egg-albumin-induced inflammation in rats. t ... | 2003 | 12963153 |
| mutation of lysine residues in apolipoprotein b-100 causes defective lipoprotein[a] formation. | lipoprotein[a] (lp[a]) is assembled by a two-step process involving an initial lysine-dependent binding between apolipoprotein b-100 (apob-100) and apolipoprotein[a] (apo[a]) that facilitates the formation of a disulphide bond between apob-100cys4,326 and apo[a]cys4,057. previous studies of transgenic mice expressing apob-95 (4,330 amino acids) and apob-97 (4,397 amino acids) have shown that apob-100 amino acids 4,330-4,397 are important for the initial binding to apo[a]. furthermore, a lysine-r ... | 2004 | 13130121 |
| the inhibitory action of some antimalarial drugs and related compounds on the hexokinase of yeast and of plasmodium berghei. | of various antimalarial compounds tested, only proguanil failed to inhibit yeast hexokinase. the metabolite of proguanil, 10,580, was an effective inhibitor. some compounds tested which were without antimalarial activity were potent inhibitors of yeast hexokinase. the degree of inhibition increased as the time during which the enzyme had been in contact with the drug increased, and the inhibitory action of mepacrine was reduced when the concentration of atp was raised. the inhibition of yeast he ... | 1957 | 13413145 |
| dietary hepatic necrosis of albino rats induced by japanese brewer's yeast. | 1957 | 13486558 | |
| heteromorphic change of adult fibroblasts by ribonucleoproteins. | cultures of subcutaneous areolar fibroblasts from adult rats, when confronted in vitro with microsomes from rat liver or kidney, are changed heteromorphically so as to resemble cultures of nervous tissue. similar effects follow exposure to the deoxycholate-insoluble fraction of microsomes, and to purified rna from rat liver or from yeast. an equivalent ribonucleoside mixture has no heteromorphic effect. the degree of heteromorphosis can be related quantitatively to the amount of rna administered ... | 1959 | 13630930 |
| comparative studies of a new subfamily of human ste20-like kinases: homodimerization, subcellular localization, and selective activation of mkk3 and p38. | the sterile-20 or ste20 family of serine/threonine kinases is a group of signaling molecules whose physiological roles within mammalian cells are just starting to be elucidated. here, in this report we present the characterization of three human ste20-like kinases with greater than 90% similarity within their catalytic domains that define a novel subfamily of ste20s. members of this kinase family include rat thousand and one (tao1) and chicken kfc (kinase from chicken). for the lack of a consens ... | 2003 | 13679851 |
| the effect of yeast on dental caries in the rat. | 1959 | 13806549 | |
| [on experimental nutrition-conditioned liver necroses. iii. effect of a necrogenic yeast diet on the respiration and the p/o quotient of rat liver tissues]. | 1960 | 13848437 | |
| primary isolation of mycoplasma organisms (pplo) from mammalian sources. | barber, t. l. (cornell university, ithaca, n.y.) and j. fabricant. primary isolation of mycoplasma organisms (pplo) from mammalian sources. j. bacteriol. 83:1268-1273. 1962.-mycoplasma (pplo) were isolated from cattle, pigs, sheep, rats, and dogs. a total of 39 isolates was obtained from 307 samples from 166 animals. of these, 24 isolates were from semen of bulls in an artificial breeding establishment; the other 15 were from various tissues of the animals mentioned. variations of two basal medi ... | 1962 | 13864846 |
| ribonucleic acid: effect on conditioned behavior in rats. | acquisition of a behavioral response motivated by shock was enhanced in rats chronically treated with yeast ribonucleic acid, and resistance to extinction was greater in rats so treated than in controls. this extends the role of ribonucleic acid to include a behavioral effect in laboratory mammals treated with a purified preparation from yeast. | 1963 | 14022783 |
| experimental evaluation in laboratory animals of anti-inflammatory analgesic drugs. | 1963 | 14163909 | |
| the biosynthesis of the benzoquinone ring of ubiquinone from p-hydroxybenzaldehyde and p-hydroxybenzoic acid in rat kidney, azotobacter vinelandii, and baker's yeast. | 1964 | 14171457 | |
| [transhydrogenation of tpnh to dpn by lactate dehydrogenase in the liver and alcohol dehydrogenase in yeast]. | 1964 | 14306579 | |
| [on the subject of the influence of ribonucleic acid from yeast on the thirty-day survival of totally irradiated rats. new research]. | 1960 | 14420057 | |
| pias1-mediated sumoylation of focal adhesion kinase activates its autophosphorylation. | focal adhesion kinase (fak) is a protein tyrosine kinase enriched in focal adhesions, which plays a critical role in integrin-dependent cell motility and survival. the crucial step in its activation is autophosphorylation on tyr-397, which promotes the recruitment of several enzymes including src family kinases and the activation of multiple signaling pathways. we found in a yeast two-hybrid screen that the n-terminal domain of fak interacted with protein inhibitor of activated stat1 (pias1). th ... | 2003 | 14500712 |
| in vitro metabolism of perospirone in rat, monkey and human liver microsomes. | in vitro metabolism of perospirone was examined with rat, monkey and human liver s9, human liver microsomes and yeast microsomes expressing human p450, using 14c labeled perospirone. with rat liver s9, the major metabolites were mx9 and id-11614, produced by cleavage at the butylene chain. however, some butylene non-cleavage and hydration of the cyclohexane ring were found, although limited in extent. unknown metabolites accounted for about 10% of the total. after incubation for 10 minutes with ... | 2003 | 14503666 |
| siah-1 facilitates ubiquitination and degradation of synphilin-1. | parkinson's disease is a common neurodegenerative disorder characterized by loss of dopaminergic neurons and appearance of lewy bodies, cytoplasmic inclusions that are highly enriched with ubiquitin. synphilin-1, alpha-synuclein, and parkin represent the major components of lewy bodies and are involved in the pathogenesis of parkinson's disease. synphilin-1 is an alpha-synuclein-binding protein that is ubiquitinated by parkin. recently, a mutation in the synphilin-1 gene has been reported in pat ... | 2003 | 14506261 |
| complexes between the lkb1 tumor suppressor, strad alpha/beta and mo25 alpha/beta are upstream kinases in the amp-activated protein kinase cascade. | the amp-activated protein kinase (ampk) cascade is a sensor of cellular energy charge that acts as a 'metabolic master switch' and inhibits cell proliferation. activation requires phosphorylation of thr172 of ampk within the activation loop by upstream kinases (ampkks) that have not been identified. recently, we identified three related protein kinases acting upstream of the yeast homolog of ampk. although they do not have obvious mammalian homologs, they are related to lkb1, a tumor suppressor ... | 2003 | 14511394 |
| the role of mvps18p in clustering, fusion, and intracellular localization of late endocytic organelles. | delivery of endocytosed macromolecules to mammalian cell lysosomes occurs by direct fusion of late endosomes with lysosomes, resulting in the formation of hybrid organelles from which lysosomes are reformed. the molecular mechanisms of this fusion are analogous to those of homotypic vacuole fusion in saccharomyces cerevisiae. we report herein the major roles of the mammalian homolog of yeast vps18p (mvps18p), a member of the homotypic fusion and vacuole protein sorting complex. when overexpresse ... | 2003 | 14517315 |
| pig-w is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor. | many cell surface proteins are anchored to a membrane via a glycosylphosphatidylinositol (gpi), which is attached to the c termini in the endoplasmic reticulum. the inositol ring of phosphatidylinositol is acylated during biosynthesis of gpi. in mammalian cells, the acyl chain is added to glucosaminyl phosphatidylinositol at the third step in the gpi biosynthetic pathway and then is usually removed soon after the attachment of gpis to proteins. the mechanisms and roles of the inositol acylation ... | 2003 | 14517336 |
| group i metabotropic glutamate receptors bind to protein phosphatase 1c. mapping and modeling of interacting sequences. | the modulation of neurotransmitter receptors by kinases and phosphatases represents a key mechanism in controlling synaptic signal transduction. however, molecular determinants involved in the specific targeting and interactions of these enzymes are largely unknown. here, we identified both catalytic gamma-isoforms of protein phosphatase 1c (pp1gamma1 and pp1gamma2) as binding partners of the group i metabotropic glutamate receptors type 1a, 5a, and 5b in yeast cells and pull-down assays, using ... | 2003 | 14519764 |
| apoptosis-associated tyrosine kinase is a cdk5 activator p35 binding protein. | a 3(')-terminal fragment of a splice variant of kiaa0641, a human homologue of apoptosis-associated tyrosine kinase (aatyk), was screened from human brain cdna libraries by a yeast two-hybrid system using a cdk5 activator p35 as a bait. the cloned cdna encoded 477 amino acids, composed of internal 458 amino acids of kiaa0641 and 19 amino acids unique to this variant after splicing, then referred to this clone as haatyks-p35bp (human aatyk short isoform-p35 binding polypeptide). using gst-fusion ... | 2003 | 14521924 |
| differential regulation of the sir2 histone deacetylase gene family by inhibitors of class i and ii histone deacetylases. | the sir2 histone deacetylase gene family consists of seven mammalian sirtuins (sirts) which are nad-dependent histone/protein deacetylases. sir2 proteins regulate, for instance, genome stability by chromatin silencing in yeast. in mammals, their function is still largely unknown. due to the nad+ dependency, sir2 might be the link between metabolic activity and histone/protein acetylation. regulation of gene expression also seems to play an important role in sir2 functions, since increasing the d ... | 2003 | 14523559 |
| analysis of sox10 mutations identified in waardenburg-hirschsprung patients: differential effects on target gene regulation. | sox10 is a member of the sox gene family related by homology to the high-mobility group (hmg) box region of the testis-determining gene sry. mutations of the transcription factor gene sox10 lead to waardenburg-hirschsprung syndrome (waardenburg-shah syndrome, ws4) in humans. a number of sox10 mutations have been identified in ws4 patients who suffer from different extents of intestinal aganglionosis, pigmentation, and hearing abnormalities. some patients also exhibit signs of myelination deficie ... | 2003 | 14523991 |
| mechanisms underlying excitatory effects of group i metabotropic glutamate receptors via inhibition of 2p domain k+ channels. | group i metabotropic glutamate receptors (mglurs) are implicated in diverse processes such as learning, memory, epilepsy, pain and neuronal death. by inhibiting background k(+) channels, group i mglurs mediate slow and long-lasting excitation. the main neuronal representatives of this k(+) channel family (k(2p) or kcnk) are task and trek. here, we show that in cerebellar granule cells and in heterologous expression systems, activation of group i mglurs inhibits task and trek channels. d-myo-inos ... | 2003 | 14532113 |
| type 1 angiotensin ii receptor-associated protein arap1 binds and recycles the receptor to the plasma membrane. | the carboxyl terminus of the type 1 angiotensin ii receptor (at(1)) plays an important role in receptor phosphorylation, desensitization, and internalization. the yeast two-hybrid system was employed to isolate proteins associated with the carboxyl terminal region of the at(1a) receptor. in the present study, we report the isolation of a novel protein, arap1, which promotes recycling of at(1a) to the plasma membrane in hek-293 cells. arap1 cdna encodes a 493-amino-acid protein and its mrna is ub ... | 2003 | 14559250 |
| in self-defence: hexokinase promotes voltage-dependent anion channel closure and prevents mitochondria-mediated apoptotic cell death. | in tumour cells, elevated levels of mitochondria-bound isoforms of hexokinase (hk-i and hk-ii) result in the evasion of apoptosis, thereby allowing the cells to continue proliferating. the molecular mechanisms by which bound hk promotes cell survival are not yet fully understood. our studies relying on the purified mitochondrial outer membrane protein vdac (voltage-dependent anion channel), isolated mitochondria or cells in culture suggested that the anti-apoptotic activity of hk-i occurs via mo ... | 2004 | 14561215 |
| expression of mammalian cytochromes p450 in yeast. | 1998 | 14577230 | |
| centrosomal anchoring of protein kinase c betaii by pericentrin controls microtubule organization, spindle function, and cytokinesis. | location is a critical determinant in dictating the cellular function of protein kinase c (pkc). scaffold proteins contribute to localization by poising pkc at specific intracellular sites. using a yeast two-hybrid screen, we identified the centrosomal protein pericentrin as a scaffold that tethers pkc betaii to centrosomes. co-immunoprecipitation studies reveal that the native proteins interact in cells. co-overexpression studies show that the interaction is mediated by the c1a domain of pkc an ... | 2004 | 14594954 |
| mals is a binding partner of irsp53 at cell-cell contacts. | insulin receptor substrate p53 (irsp53) is a key player in cytoskeletal dynamics, interacting with the actin modulators wave2 and mena. here, we identified a pdz protein, mals, as an irsp53-interacting protein using a yeast two-hybrid screen. a pull-down assay showed that irsp53 and mals interact through the pdz domain of mals and the c-terminal pdz-binding sequence of irsp53. their interaction in mdck cells was also demonstrated by co-immunoprecipitation. immunocytochemistry showed the colocali ... | 2003 | 14596909 |
| lkb1 is the upstream kinase in the amp-activated protein kinase cascade. | inactivating mutations in the protein kinase lkb1 lead to a dominantly inherited cancer in humans termed peutz-jeghers syndrome. the role of lkb1 is unclear, and only one target for lkb1 has been identified in vivo [3]. amp-activated protein kinase (ampk) is the downstream component of a protein kinase cascade that plays a pivotal role in energy homeostasis. ampk may have a role in protecting the body from metabolic diseases including type 2 diabetes, obesity, and cardiac hypertrophy. we previou ... | 2003 | 14614828 |
| use of an "acetaldehyde clamp" in the determination of low-km aldehyde dehydrogenase activity in h4-ii-e-c3 rat hepatoma cells. | the high-affinity (k(m)<1 microm) mitochondrial class 2 aldehyde dehydrogenase (aldh2) metabolizes most of the acetaldehyde generated in the hepatic oxidation of ethanol. h4-ii-e-c3 rat hepatoma cells have been found to express aldh2. we report a method to assess aldh2 activity in intact hepatoma cells that does not require mitochondrial isolation. to determine only the high-affinity aldh2 activity it is necessary to keep constant low concentrations of acetaldehyde in the cells to minimize its m ... | 2003 | 14615007 |
| cloning and expression of the major secreted cathepsin b-like protein from juvenile fasciola hepatica and analysis of immunogenicity following liver fluke infection. | the functions of the cathepsin b-like proteases in liver flukes are unknown and analysis has been hindered by a lack of protein for study, since the protein is produced in small amounts by juvenile flukes. to circumvent this, we isolated and characterized a cdna encoding the major secreted cathepsin b from fasciola hepatica. the predicted preproprotein is 339 amino acids in length, with the mature protease predicted to be 254 amino acids long, and shows significant similarity to parasite and mam ... | 2003 | 14638781 |
| endophilins interact with moloney murine leukemia virus gag and modulate virion production. | the retroviral gag protein is the central player in the process of virion assembly at the plasma membrane, and is sufficient to induce the formation and release of virus-like particles. recent evidence suggests that gag may co-opt the host cell's endocytic machinery to facilitate retroviral assembly and release. | 2003 | 14659004 |
| production of phosphatidylinositol 5-phosphate by the phosphoinositide 3-phosphatase myotubularin in mammalian cells. | mtm1, the gene encoding myotubularin (mtm1), is mutated in the x-linked myotubular myopathy (xlmtm), a severe genetic muscular disorder. mtm1 is a phosphoinositide phosphatase hydrolyzing phosphatidylinositol 3-phosphate (ptdins(3)p) in yeast and in vitro. because this lipid is implicated in the regulation of vesicular trafficking, we used established cell lines from xlmtm patients to evaluate whether the lack of endogenous mtm1 expression could affect ptdins(3)p labeling patterns. our results s ... | 2004 | 14660569 |
| safety evaluation of ice-structuring protein (isp) type iii hplc 12 preparation. lack of genotoxicity and subchronic toxicity. | ice-structuring proteins (isps) naturally occur in a range of species (including edible plants and fish) that need to protect themselves against freeze damage. isps have potential applications in a number of areas including cryopreservation and frozen foods manufacture. however, these materials are not currently generally available for commercial use. isp type iii hplc 12 is of particular interest and although it is likely to be consumed naturally, its toxicological safety has not previously bee ... | 2004 | 14667477 |
| characterizing gene sets with funcassociate. | summary: funcassociate is a web-based tool to help researchers use gene ontology attributes to characterize large sets of genes derived from experiment. distinguishing features of funcassociate include the ability to handle ranked input lists, and a monte carlo simulation approach that is more appropriate to determine significance than other methods, such as bonferroni or idák p-value correction. funcassociate currently supports 10 organisms (vibrio cholerae, shewanella oneidensis, saccharomyces ... | 2003 | 14668247 |
| mammalian late vacuole protein sorting orthologues participate in early endosomal fusion and interact with the cytoskeleton. | in saccharomyces cerevisiae, the class c vacuole protein sorting (vps) proteins, together with vam2p/vps41p and vam6p/vps39p, form a complex that interacts with soluble n-ethylmaleimide-sensitive factor attachment protein receptor and rab proteins to "tether" vacuolar membranes before fusion. to determine a role for the corresponding mammalian orthologues, we examined the function, localization, and protein interactions of endogenous mvps11, mvps16, mvps18, mvam2p, and mvam6. we found a signific ... | 2004 | 14668490 |
| isolation and characterization of an rip (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity. | ribosome-inactivating proteins (rips) are n-glycosidases that remove a specific adenine from the sarcin/ricin loop of the large rrna, thus arresting protein synthesis at the translocation step. in the present study, a protein termed tobacco rip (trip) was isolated from tobacco (nicotiana tabacum) leaves and purified using ion exchange and gel filtration chromatography in combination with yeast ribosome depurination assays. trip has a molecular mass of 26 kd as evidenced by sodium dodecyl sulfate ... | 2004 | 14671015 |
| identification of the human mitochondrial s-adenosylmethionine transporter: bacterial expression, reconstitution, functional characterization and tissue distribution. | the mitochondrial carriers are a family of transport proteins that, with a few exceptions, are found in the inner membranes of mitochondria. they shuttle metabolites and cofactors through this membrane, and connect cytoplasmic functions with others in the matrix. sam (s-adenosylmethionine) has to be transported into the mitochondria where it is converted into s-adenosylhomocysteine in methylation reactions of dna, rna and proteins. the transport of sam has been investigated in rat liver mitochon ... | 2004 | 14674884 |
| cyclin-dependent kinase 11p110 and casein kinase 2 (ck2) inhibit the interaction between tyrosine hydroxylase and 14-3-3. | tyrosine hydroxylase (th) is regulated by the reversible phosphorylation of serines 8, 19, 31 and 40. upon initiation of this study, serine 19 was unique due to its requirement of 14-3-3 binding after phosphorylation for optimal enzyme activity, although it has been more recently demonstrated that phosphorylated serine 40 also binds 14-3-3. to identify proteins that interact with th following phosphorylation of serine 19, this amino acid was mutated to alanine and ths19a was used as bait in a ye ... | 2004 | 14675149 |
| inhibitory regulation of cystic fibrosis transmembrane conductance regulator anion-transporting activities by shank2. | accumulating evidence suggests that protein-protein interactions play an important role in transepithelial ion transport. in the present study, we report on the biochemical and functional association between cystic fibrosis transmembrane conductance regulator (cftr) and a pdz domain-containing protein shank2. exploratory reverse transcription-pcr screening revealed mrnas for several members of pdz domain-containing proteins in epithelial cells. shank2, one of these scaffolding proteins, showed a ... | 2004 | 14679199 |
| mouse mafa, homologue of zebrafish somite maf 1, contributes to the specific transcriptional activity through the insulin promoter. | large maf transcription factors, which are members of the basic leucine zipper (b-zip) superfamily, have been reported to be involved in embryonic development and cell differentiation. previously, we isolated a novel zebrafish large maf cdna, somite maf1 (smaf1), which possesses transactivational activity within its n-terminus domain. to elucidate smaf1 function in mammals, we tried to isolate the mouse homologue of zebrafish smaf1. we isolated the mouse homologue of zebrafish smaf1, which is th ... | 2003 | 14680841 |
| germonline, a cross-species community knowledgebase on germ cell differentiation. | germonline provides information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species. the database has been developed, and is being curated and updated, by life scientists in cooperation with bioinformaticists. information is contributed through an online form using free text, images and the controlled vocabulary developed by the geneontology consortium. authors provide up to three references in support of their contr ... | 2004 | 14681481 |
| gamma-cop appendage domain - structure and function. | copi-coated vesicles mediate retrograde transport from the golgi back to the er and intra-golgi transport. the cytosolic precursor of the copi coat, the heptameric coatomer complex, can be thought of as composed of two subcomplexes. the first consists of the beta-, gamma-, delta- and zeta-cop subunits which are distantly homologous to ap clathrin adaptor subunits. the second consists of the alpha-, beta'- and epsilon-cop subunits. here, we present the structure of the appendage domain of gamma-c ... | 2004 | 14690497 |
| an abundant placental transcript containing an iap-ltr is allelic to mouse pregnancy-specific glycoprotein 23 (psg23): cloning and genetic analysis. | several families of endogenous retroviruses (ervs) are expressed in mammalian placental tissues, and are implicated in aspects of placental development and function. we characterized the structure of abundant erv-related transcripts in mouse placenta. in addition to the 7 kb full-length type i and 5 kb type i deleted intracisternal a-particle (iap) transcripts, we identified and cloned an abundant 2 kb transcript encoding a novel member of the pregnancy-specific glycoprotein (psg) gene family, w ... | 2004 | 14697515 |
| marlin-1, a novel rna-binding protein associates with gaba receptors. | gaba(b) receptors are heterodimeric g protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. whereas heterodimerization between gaba(b) receptor gaba(b)r1 and gaba(b)r2 subunits is essential for functional expression, how neurons coordinate the assembly of these critical receptors remains to be established. here we have identified marlin-1, a novel gaba(b) receptor-binding protein that associates specifically with the gaba(b)r1 subunit in yeast, tissue cul ... | 2004 | 14718537 |
| novel neuroprotective k+ channel inhibitor identified by high-throughput screening in yeast. | discovery of k+ channel modulators is limited by low-throughput capacity of existing k+ channel assays. to enable high-throughput screening for novel pharmacological modulators of k+ channels, we developed an assay based on growth of yeast that functionally expresses mammalian kir2.1 channels. screening of 10,000 small molecules from a combinatorial chemical library yielded 42 potential kir2.1 inhibitors. one compound, 3-bicyclo[2.2.1]hept-2-yl-benzene-1,2-diol, was confirmed to inhibit k+ chann ... | 2004 | 14722253 |
| the nci/cit microarray database (madb) system - bioinformatics for the management and analysis of affymetrix and spotted gene expression microarrays. | a scalable, modular, enterprise-level system for both microarray databasing and analysis over the internet has been developed over the past four years by the national cancer institute's center for cancer research in collaboration with nih's center for information technology. this completely web-based system, called madb (for microarray database), is currently supporting over 810 registered users and collaborators at nih and contains over 22,000 microarray experiments, making it one of the larges ... | 2003 | 14728569 |
| the glucocorticoid receptor and the orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor ii interact with and mutually affect each other's transcriptional activities: implications for intermediary metabolism. | glucocorticoids exert their metabolic effect via their intracellular receptor, the glucocorticoid receptor (gr). in a yeast two-hybrid screening, we found the chicken ovalbumin upstream promoter transcription factor ii (coup-tfii), an orphan nuclear receptor that plays important roles in glucose, cholesterol, and xenobiotic metabolism, as a partner of gr. in an in vitro glutathione-s-transferase pull-down assay, coup-tfii interacted via its dna-binding domain with the hinge regions of both gralp ... | 2004 | 14739255 |