Publications

reinterpretation of the ractk1 k+ channel.	the ractk1 cdna cloned from rabbit kidney cortical collecting duct cells was associated with inwardly rectifying ph-regulated k+ channel activity (m. suzuki, k. takahashi, m. [keda, h hayakawa, a. ogawa, y. kawaguchi, and o. sakai. nature lond. 367: 642-645, 1994). the deduced amino acid sequence of the encoded novel polypeptide lacked the signature sequence of a k(+)-selective pore region but predicted a topography suggestive of the inward rectifier k+ channel family. in subsequent articles a r ...	1997	9038842
similar sequence in e. coli.		1997	9038843
expression, purification and immunochemical characterization of recombinant bovine beta-lactoglobulin, a major cow milk allergen.	the immunological characteristics of a recombinant beta-lactoglobulin were studied using monoclonal antibodies, polyclonal antiserum and sera from allergic patients. recombinant beta-lactoglobulin (rblg) was expressed in escherichia coli strain dh5alpha and purified as described previously [cho et al. (1994) j. biol. chem. 269, 11 102-11 107]. the method has been modified by adding an immunoaffinity purification step. a quantity of 5-10mg of purified rblg per liter of medium culture can be produ ...	1996	9047377
rice ragged stunt oryzavirus genome segment 9 encodes a 38 600 mr structural protein.	the complete nucleotide sequence of rice ragged stunt virus genome segment 9 (s9) was determined. the s9 segment is 1132 nucleotides long and has a long open reading frame starting from the first aug codon at nucleotide position 14-16 and terminating at a uag codon located at 1028-1030, which could encode a polypeptide with an mr of 38 600 (p9). the encoded polypeptide has no sequence homology to polypeptides of any other plant reoviruses published previously. an immunological study demonstrated ...	1995	9049345
development of polyclonal antibodies for detection of aflatoxigenic molds involving culture filtrate and chimeric proteins expressed in escherichia coli.	polyclonal antibodies (pab) were raised against an aflatoxigenic strain of aspergillus parasiticus by using two different sources for antibody elicitation: (i) filtrate of a culture on which the fungus had been grown (ii) and two chimeric proteins, expressed in escherichia coli as separate products, of the genes ver-1 and apa-2, which are involved in aflatoxin biosynthesis. the gene products were amplified by pcr, and each was cloned into the e. coli expression vector pgex2t. upon induction, the ...	1997	9055416
synthesis of 4- and 5-series leukotrienes in the lung microvasculature challenged with escherichia coli hemolysin: critical dependence on exogenous free fatty acid supply.	escherichia coli hemolysin (hlya) has been identified as a potent inductor of phosphoinositide hydrolysis and related metabolic responses in neutrophils (grimminger and colleagues, 1991, j. clin. invest. 88:1531-1539). in isolated perfused rabbit lungs, which harbor a large number of entrapped microvascular leukocytes, we investigated the effect of a low dose of hlya on lipoxygenase product formation in the presence of exogenous free arachidonic acid (aa), eicosapentaenoic acid (epa), or both pr ...	1997	9070617
large-scale preparation and characterization of recombinant ovine placental lactogen.	to clone ovine placental lactogen (opl) cdna, total rna from sheep placental cotyledon was reverse transcribed and the single-stranded cdna was pcr-amplified with 5' and 3' primers containing, respectively, ncoi and psti sites. the opl cdna fragment amplified between these two primers extended from a(-1) to the natural stop codon. the pcr product was gel-purified and subcloned into a puc vector and the insert was sequenced on both strands, revealing several differences relative to the published ...	1997	9071989
role of glutamine in bacterial transcytosis and epithelial cell injury.	l-glutamine is the principal energy source for small intestinal enterocytes. diminution of intestinal function, mucosal atrophy, and increased bacterial translocation have been noted during total parenteral nutrition (tpn). in a rat model of glutamine starvation, we previously showed that luminal glutamine is essential for optimal intestinal function. in this study, we examined the effect of apical vs basolateral glutamine on bacterial translocation in a caco-2 cell culture system and bacteria-i ...	1997	9084009
prevalence and characteristics of enteropathogenic escherichia coli with the eae gene in diarrhoeic rabbits.	a field study was carried out with the objective of investigating the prevalence of enteropathogenic escherichia coli (epec) with the eae gene in diarrhoeic rabbits. epec eae+ were isolated from 60 (74%) of 81 diarrhoeic rabbits sampled in 30 industrial fattening farms localized in the four provinces of galicia (northwestern spain). attaching and effacing lesions were found in 44 of 50 animals processed for histology. the 111 e. coli strains identified belonged to 19 different o serogroups and 1 ...	1997	9087949
studies by site-directed mutagenesis of the carbohydrate-binding properties of a bark lectin from robinia pseudoacacia.	a bark lectin, rbl, from robinia pseudoacacia (black locust), binds galactose-related sugars specifically. recombinant rbl (rrbl) with a histidine tag was expressed in escherichia coli, purified and characterized. rrbl agglutinated rabbit erythrocytes and the hemagglutination was inhibited by galactose and related sugars. to elucidate the mechanism of the binding of carbohydrate by rbl, 16 mutant rrbls were produced by site-directed mutagenesis. the analysis of the mutants indicated that residue ...	1997	9091320
ca2+-dependent membrane bound protein fraction from rabbit gastric mucosa contains a protein whose histidyl residue is phosphorylated.	we found an autophosphorylated protein with a molecular weight of 40 kda (p40) in the crude annexin fraction of rabbit gastric mucosa, i.e., the materials released by egta from the membrane fraction obtained in the presence of ca2+. this protein was enriched in chief cells in the gastric glands, and also found in the heart and the liver by western blotting. the protein bound to phenyl-sepharose in the presence of ca2+ and showed extremely basic nature. the phosphorylation site of p40 was conside ...	1997	9099993
plant immunomodulators for termination of unwanted pregnancy and for contraception and reproductive health.	neem (azadirachta indica) seed and leaf extracts have spermicidal, anti-microbial, anti-fungal and anti-viral properties. they are also immunomodulators that induce primarily a th1 type response. these properties are being exploited to develop two different useful methods of fertility control. neem extracts given orally at early post-implantation stage terminate pregnancy in rodents and primates. treatment has no residual permanent effect and fertility is regained in subsequent cycles. the mecha ...	1997	9107574
oxa1p, which is required for cytochrome c oxidase and atp synthase complex formation, is embedded in the mitochondrial inner membrane.	we have previously isolated the yeast nuclear gene oxa1 and showed that oxa1p is required for the formation of the cytochrome c oxidase and atp synthase complexes. we have expressed oxa1p in e. coli and shown that it is toxic and rapidly degraded. nevertheless, a truncated protein was successfully expressed and antibodies have been raised against this truncated protein. these antibodies recognise a protein in mitochondrially enriched fractions. in vitro mitochondrial import experiments demonstra ...	1997	9108137
progress towards using recombinant myxoma virus as a vector for fertility control in rabbits.	the history of myxoma virus, its use in australia as a mortality agent and the development of the virus as a vector for controlling fertility in wild rabbit populations in australia is reviewed. myxoma virus recombinants have been constructed to express model antigens. four potential insertion sites in the genome have been identified and two have been used to construct single and double recombinant viruses expressing escherichia coli enzymes beta-galactosidase and beta-glucuronidase. another rec ...	1997	9109196
a function for the qkraa amino acid motif: mediating binding of dnaj to dnak. implications for the association of rheumatoid arthritis with hla-dr4.	the amino acid motif qkraa, when expressed on hla-drb1, carries susceptibility to develop rheumatoid arthritis. this motif is the basis of strong b and t cell epitopes. furthermore, it is highly overrepresented in protein databases, suggesting that it carries a function of its own. to identify this function, we used qkraa peptide affinity columns to screen total protein extracts from escherichia coli. we found that dnak, the e. coli 70-kd heat shock protein, binds qkraa. of interest, dnak has a ...	1997	9109425
prevention of mucosal escherichia coli infection by fimh-adhesin-based systemic vaccination.	virtually all uropathogenic strains of escherichia coli, the primary cause of cystitis, assemble adhesive surface organelles called type 1 pili that contain the fimh adhesin. sera from animals vaccinated with candidate fimh vaccines inhibited uropathogenic e. coli from binding to human bladder cells in vitro. immunization with fimh reduced in vivo colonization of the bladder mucosa by more than 99 percent in a murine cystitis model, and immunoglobulin g to fimh was detected in urinary samples fr ...	1997	9110982
characterization of p0, a ribosomal phosphoprotein of plasmodium falciparum. antibody against amino-terminal domain inhibits parasite growth.	a cdna expression clone of the human malarial parasite plasmodium falciparum, lambdapf4, which was reactive only to the immune sera and not to the patient sera, has recently been found to be the p. falciparum homologue of the p0 ribosomal phosphoprotein gene. a northern analysis of the p0 gene revealed the presence of two transcripts, both present in all the different intraerythrocytic stages of the parasite life cycle. a 138-base pair amino-terminal domain of this gene was expressed as a fusion ...	1997	9115284
molecular, biological, and preliminary structural analysis of recombinant bryodin 1, a ribosome-inactivating protein from the plant bryonia dioica.	bryonia dioica (cucurbitaceae family) produces at least two type i ribosome-inactivating proteins, bryodin 1 (bd1) and bryodin 2 (bd2). a cdna sequence encoding bd1 was isolated from b. dioica leaf mrna using degenerative oligonucleotides and codes for a 22 amino acid signal peptide followed by a protein of 267 residues. expression of two recombinant bd1 (rbd1) forms in escherichia coli yielded proteins of 267 (to the natural stop codon) and 247 amino acids (to the putative cleavage site yieldin ...	1997	9115985
molecular cloning and expression of subunit 9 of the 26s proteasome.	seven peptides from subunit 9 (s9) of the human 26s proteasome were sequenced and this information was used to clone a hela cdna that encodes the 46 kda subunit. rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from s9 and against a full-length s9 expressed in e. coli. western blot analysis showed that the s9-specific antibodies bound the 26s proteasome and its regulatory complex separated on non-denaturing gels. in sds-page samples of the two com ...	1997	9119060
effects of low power laser-irradiation on differential blood count and body temperature in endotoxin-preimmunized rabbits.	low power laser irradiation has been shown to have various immune-modulatory effects under in vitro conditions but little is known about such effects in animal models. escherichia coli endotoxin-preimmunized rabbits were used to determine the influence of transcutaneously applied low power laser light on differential blood count and rectal temperature. after three initial immunizations animals were either boostered with 5 ng/kg of endotoxin or injected with pyrogen-free saline and subsequently u ...	1997	9129122
analysis of epitope structure of psp94 (prostate secretory protein of 94 amino acids): (i). immuno-dominant and immuno-recessive area.	psp94 is a potential biomarker for evaluating patients with prostate carcinoma. we have systematically studied the epitope structure of psp94 by using a polyclonal antibody against human psp94. results of peptide mapping and elisa tests of dose response to rabbit antiserum against human psp94 protein showed that only the n-terminal peptides (n30 and m23) are immunoreactive while all the synthetic peptides (c28, c10) located closer to the c-terminus are completely devoid of antigenic activity wit ...	1997	9136076
antibody reactivities to tumor-suppressor protein p53 and htlv-i tof, rex and tax in htlv-i-infected people with differing clinical status.	since the presence of anti-p53 antibody has been correlated with the mutation and accumulation of p53, the aim of this study was to detect anti-p53 antibody and understand its correlations with anti-tof, -rex, or -tax antibody reactivity in htlv-i infected people differing in their clinical status. a plasmid (pgex-tof) was constructed to express tof recombinant protein (rp) in escherichia coli. serum samples from 50 asymptomatic carriers (acs), 50 adult t-cell leukemia (atl) and 50 htlv-i-associ ...	1997	9139842
ono-5046, an elastase inhibitor, attenuates endotoxin-induced acute lung injury in rabbits.	endotoxin causes acute lung injury resembling acute respiratory distress syndrome. elastase, as well as reactive oxygen species released from activated neutrophils, are thought to play pivotal roles in the pathogenesis of this lung injury. this study investigated whether ono-5046, a specific elastase inhibitor, can attenuate acute lung injury induced by endotoxin in rabbits. thirty-two male anesthetized rabbits were randomly assigned to receive one of four treatments (n = 8 for each group): infu ...	1997	9141938
efficient autoproteolytic processing of the mhv-a59 3c-like proteinase from the flanking hydrophobic domains requires membranes.	the replicase gene of the coronavirus mhv-a59 encodes a serine-like proteinase similar to the 3c proteinases of picornaviruses. this proteinase domain is flanked on both sides by hydrophobic, potentially membrane-spanning, regions. cell-free expression of a plasmid encoding only the 3c-like proteinase (3clpro) resulted in the synthesis of a 29-kda protein that was specifically recognized by an antibody directed against the carboxy-terminal region of the proteinase. a protein of identical mobilit ...	1997	9143287
characterization of a large transferrin-binding protein from actinobacillus pleuropneumoniae serotype 7.	the binding of transferrin at the surface of actinobacillus pleuropneumoniae (a. pp.) is mediated by two proteins of approximately 60 and 100 kda. the 60 kda protein has been shown to be highly divergent among different serotypes and to induce a serotype-specific protective immune response. in this study we have characterized the 100 kda transferrin-binding protein of a. pp. serotype 7 and designated it as tfbb. the tfbb gene was found to be located immediately downstream of the tfba gene. it wa ...	1997	9151535
accessory subunit of mitochondrial dna polymerase from drosophila embryos. cloning, molecular analysis, and association in the native enzyme.	a full-length cdna of the accessory (beta) subunit of mitochondrial dna polymerase from drosophila embryos has been obtained, and its nucleotide sequence was determined. the cdna clone encodes a polypeptide with a deduced amino acid sequence of 361 residues and a predicted molecular mass of 41 kda. the gene encoding the beta subunit lies within 4 kilobase pairs of that for the catalytic subunit in the drosophila genome, on the left arm of chromosome 2. the two genes have similar structural featu ...	1997	9153213
delivery of primary autologous skeletal myoblasts into rabbit heart by coronary infusion: a potential approach to myocardial repair.	myocardial repair after injury is limited because the adult heart cannot regenerate. we propose using autologous skeletal muscle cells (myoblasts) as a source of reserve cells for repair of regions of damaged myocardium. this report examines two potential methods for the transfer of cells to the myocardium: selective coronary catheterization, and myoblast infusion or myoblast injection directly into the left ventricular wall. autologous, primary rabbit skeletal myoblasts were harvested, were tra ...	1997	9154641
a third secreted protein that is encoded by the enteropathogenic escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells.	enteropathogenic escherichia coli strains are able to signal host cells, cause dramatic cytoskeletal rearrangements, and adhere intimately to the cell surface in a process known as the attaching and effacing effect. a pathogenicity island of 35 kb known as the locus of enterocyte effacement (lee) is necessary and sufficient for this effect. the lee encodes an outer membrane adhesin called intimin, a type iii secretion apparatus, and the espa and espb secreted proteins. the dna sequence of the re ...	1997	9169753
prelytic and lytic conformations of erythrocyte-associated escherichia coli hemolysin.	flow cytometry was developed as a method to assess the conformation of erythrocyte-bound escherichia coli hemolysin polypeptide (hlya). topology of membrane-associated hemolysin (hlya(e)) was investigated by testing surface accessibility of hlya regions in lytic and nonlytic bound states, using a panel of 12 anti-hlya monoclonal antibodies (mabs). hemolysin associates nonlytically with erythrocytes at 0 to 2 degrees c. to test the hypothesis that the nonlytic hlya(e) conformation at 0 to 2 degre ...	1997	9169756
cdna cloning, expression, and rapid purification of a kunitz-type winged bean chymotrypsin inhibitor.	a 183-residue kunitz-type winged bean chymotrypsin inhibitor (wbci), inhibits its cognate protease at a molar ratio of 1:2, instead of the usual ratio of 1:1 common to other members of the family. from the cdna pool obtained by reverse transcription of the poly(a)+ rna of the developing winged bean seeds, the structural gene of wbci has been amplified by pcr using primers designed to delete the 24-residue signal peptide and introduce ecori and sali sites at the ends of the amplified dna. the lat ...	1997	9179296
structural basis for differential receptor binding of cholera and escherichia coli heat-labile toxins: influence of heterologous amino acid substitutions in the cholera b-subunit.	the closely related b-subunits of cholera toxin (ctb) and escherichia coli heat-labile enterotoxin (ltb) both bind strongly to gm1 ganglioside receptors but ltb can also bind to additional glycolipids and glycoproteins. a number of mutant ct b-subunits were generated by substituting ctb amino acids with those at the corresponding positions in ltb. these were used to investigate the influence of specific residues on receptor-binding specificity. a mutated ctb protein containing the first 25 resid ...	1997	9179843
release of replication-deficient retroviruses from a packaging cell line: interaction with glioma tumor spheroids in vitro.	the present study describes how various growth conditions affect gene expression and virus production from a retroviral packaging cell line (liz 9), grown as monolayers and as multicellular spheroids. in addition, to study the direct interaction between packaging cells and tumor tissue of glioma origin, liz 9 spheroids were confronted with tumor spheroids derived from a human glioma cell line, gamg. the results show a progressive gene transfer into the tumor tissue, with 9% transfection efficacy ...	1997	9180159
localization of the in vivo expression of p and f1 fimbriae in chickens experimentally inoculated with pathogenic escherichia coli.	escherichia coli causing septicemia in poultry often possess f1 (type 1) and/or p fimbriae which may be involved in bacterial colonization and infection. to investigate the expression of these fimbriae in vivo, two pathogenic e. coli strains with different fimbrial profiles, tk3 (fim+/pap+) and mt78 (fim+/pap-), were administered to 2-week-old chickens by either the intratracheal or caudal thoracic air sac inoculation route. antibodies specific for native f1 fimbriae were detected by elisa and i ...	1997	9188088
a putative monofunctional glycosyltransferase is expressed in ralstonia eutropha.	a gene, mgt, encoding a protein homologous to the n-terminal module of class a high-molecular-mass penicillin-binding proteins was identified in ralstonia eutropha. by using specific antibodies, the corresponding mgt protein was detected in association with the membrane, confirming that the n-terminal hydrophobic segment functioned as a membrane anchor. a derivative in which the hydrophobic sequence was deleted was overexpressed as a maltose-binding fusion protein in escherichia coli. cleavage o ...	1997	9190828
immunological cross reactivity of eaea (intimin) from e. coli that cause attaching and effacing lesions in humans and rabbits.		1997	9192001
binding of human enterotoxigenic escherichia coli expressing coli surface antigen 6 to rabbit intestinal enterocytes and glycoproteins.		1997	9192024
af/r2 adhesin and cytopathic effect as virulence traits of diarrhea-inducing escherichia coli o103 in european rabbit.		1997	9192028
hemolysin phenotypes and genotypes of eaea-positive and eaea-negative bovine verotoxigenic escherichia coli.	intimin or eaea protein has been implicated in the attaching/effacing lesion caused by entero-hemorrhagic escherichia coli (ehec) in the intestine but it is not produced by all ehec and is therefore not adequate as a marker for ehec. hemolysins are produced by a high percentage of verotoxigenic e. coli (vtec) and could be a marker for ehec, but their distribution and relation to virulence are not known. we used pcr amplification to determine the presence or absence of eaea sequences in 281 vtec ...	1997	9192033
localization of intravenously administered verocytotoxins (shiga-like toxins) 1 and 2 in rabbits immunized with homologous and heterologous toxoids and toxin subunits.	rabbits challenged intravenously with shiga toxin or with escherichia coli verocytotoxin 1 or 2 (vt1 or vt2) are known to develop diarrhea, paralysis, and death, which can be prevented by immunization with a toxoid. the pathological effects of vt1 in the central nervous system and the gastrointestinal tract of unimmunized rabbits correlate with the localization of 125i-vt1 in these tissues, whereas in immunized animals, localization of 125i-vt1 in target tissues is inhibited and labeled toxin is ...	1997	9199412
intranasal immunogenicity and adjuvanticity of site-directed mutant derivatives of cholera toxin.	genetically modified derivatives of cholera toxin (ct), harboring a single amino acid substitution in and around the nad binding cleft of the a subunit, were isolated following site-directed mutagenesis of the ctxa gene. two mutants of ct, designated cts106 (with a proline-to-serine change at position 106) and ctk63 (with a serine-to-lysine change at position 63), were found to have substantially reduced adp-ribosyltransferase activity and toxicity; ctk63 was completely nontoxic in all assays, w ...	1997	9199455
mutational analysis of substrate recognition by protein phosphatase 1.	the role of residues that are involved in substrate recognition by rabbit muscle protein phosphatase 1alpha (pp1) was investigated by site-directed mutagenesis and kinetic analyses using phosphorylase a, rii peptide, kemptide, and p-nitrophenyl phosphate as substrates. the atomic structure of pp1 has shown the active site to be at the confluence of three shallow grooves, a c-terminal groove, an acidic groove, and a hydrophobic groove. mutations of residues d208, d210, d212, e218, d220, e252, d25 ...	1997	9204865
microbial growth inside saline-filled breast implants.	in vitro and in vivo experiments were conducted to determine whether intraluminal saline in breast implants can support the growth of common wound-infecting microorganisms over a prolonged period of time. the bacteria tested were staphylococcus aureus, staphylococcus epidermidis, escherichia coli, corynebacterium jeikeium, enterobacter cloacae, klebsiella pneumoniae, and pseudomonas aeruginosa. three fungal species also were tested: aspergillus fumigatus, paecilomyces variotii, and candida albic ...	1997	9207676
identification of a fourth gene involved in dtdp-rhamnose synthesis in streptococcus mutans.	we had isolated three genes (rmla, rmlb, and rmlc) involved in dtdp-rhamnose synthesis in streptococcus mutans and found that three genes were insufficient for dtdp-rhamnose synthesis (y. tsukioka, y. yamashita, t. oho, y. nakano, and t. koga, j. bacteriol. 179:1126-1134, 1997). the rmld gene of s. mutans, encoding the enzyme which catalyzes the last step of dtdp-rhamnose synthesis, has been cloned and sequenced. the cell extract of escherichia coli expressing the rmld gene of s. mutans exhibite ...	1997	9209063
expression, purification, and enzymatic properties of recombinant human cytochrome p450c27 (cyp27).	a large number of microsomal p450s have been expressed in escherichia coli in quantities sufficient for structure/function analysis. however, only one mitochondrial p450 has been successfully overexpressed, that being cholesterol side chain cleavage cytochrome p450 (p450scc). we report here overexpression, purification, and characterization of a second mitochondrial p450, human sterol c-27 hydroxylase (p450c27). the conditions used for expression are very similar to those applied for p450scc, al ...	1997	9210654
cloning and analysis of a borrelia burgdorferi membrane-interactive protein exhibiting haemolytic activity.	we cloned the gene encoding a membrane-interactive protein of borrelia burgdorferi by means of its haemolytic activity in escherichia coli. the haemolytic activity was erythrocyte-species specific, with progressively decreasing activity for erythrocytes from horse, sheep, and rabbit, respectively. genetic analysis of the haemolytic determinant revealed two borrelia haemolysin genes, blya and blyb, that are part of a predicted four-gene operon which is present in multiple copies on the 30 kb circ ...	1997	9218769
dna-binding activity of the a-factor receptor protein and its recognition dna sequences.	the a-factor receptor protein (arpa) containing an alpha-helix-turn-alpha-helix dna-binding consensus sequence at its n-terminal portion plays a key role in the regulation of secondary metabolism and cell differentiation in streptomyces griseus. a binding site forming a palindrome 24bp in length was initially recovered from a pool of random-sequence oligonucleotides by rounds of a binding/immunoprecipitation/amplification procedure with histidine-tagged arpa and anti-arpa antibody. by means of f ...	1997	9220006
selective modification at the n-terminal region of human growth hormone that shows antagonistic activity.	a new analogue of recombinant human growth hormone (hgh), hgh des(1-6,14) was expressed in escherichia coli, refolded and purified to homogeneity. the mutation decreased the hormone's ability to bind lactogenic and somatogenic receptors through its site 1, and almost completely abolished its ability to bind these receptors through site 2, as evidenced by both binding and gel-filtration experiments. more specifically, the binding to prolactin receptors (prlrs) from various species or their solubl ...	1997	9220030
histidine-44 of the a subunit of escherichia coli enterotoxin is involved in its enzymatic and biological activities.	we examined the role in toxicity of histidine-44 of the a subunit of escherichia coli enterotoxin, which is located in the active site cavity close to glutamic acid-112. although amino acid substitution of histidine-44 usually renders a mutant toxin unstable to trypsin, one mutant, alanine-44 (his44ala) was found to be stable. his44ala did not show any agmatine:adp-ribosyltransferase activity in the presence or absence of recombinant adp-ribosylation factor. it showed no diarrheal or rabbit skin ...	1997	9231414
characterization of a recombinant fragment that contains a carbohydrate recognition domain of the filamentous hemagglutinin.	the filamentous hemagglutinin (fha) of bordetella pertussis plays an important role in establishing infection by attaching the bacteria to the ciliated respiratory epithelial cells. expression of dna encoding residues 1141 to 1279 of fha in escherichia coli yields a protein of 18,000 da that exhibits some of the carbohydrate recognition properties of fha (s. m. prasad, y. yin, e. rodzinski, e. i. tuomanen, and h. r. masure, infect. immun. 61:2780-2785, 1993). we have constructed an e. coli strai ...	1997	9234814
conformational changes in actin induced by its interaction with gelsolin.	actin cleaved by the protease from escherichia coli a2 strain between gly42 and val43 (ecp-actin) is no longer polymerizable when it contains ca2+ as a tightly bound cation, but polymerizes when mg2+ is bound. we have investigated the interactions of gelsolin with this actin with regard to conformational changes in the actin molecule induced by the binding of gelsolin. ecp-(ca)actin interacts with gelsolin in a manner similar to that in which it reacts with intact actin, and forms a stoichiometr ...	1997	9251809
localization of radiolabeled chemotactic peptide at focal sites of escherichia coli infection in rabbits: evidence for a receptor-specific mechanism.	the infection imaging properties of a high-affinity 99mtc-labeled chemotactic peptide receptor agonist (n-formyl-methionyl-leucyl-phenylalanine-lysine; n-for-mlfk) were compared with a low-affinity agonist (n-acetyl-mlfk; n-ac-mlfk), a moderate-affinity antagonist (n-isobutyloxycarbonyl-mlfk; n-iboc-mlfk) and non-specific inflammation imaging agents.	1997	9255175
effect of lipid modification on the physicochemical, structural, antigenic and immunoprotective properties of haemophilus influenzae outer membrane protein p6.	the outer membrane lipoprotein, p6 of haemophilus influenzae was studied to determine the importance of the native palmitoyl moiety on its physicochemical and immunological properties. a recombinant p6 (rp6) molecule devoid of lipidation signal sequence was expressed in escherichia coli and its properties were compared to those of the palmitylated protein purified from h. influenzae. the isoelectric point of rp6 was more acidic than that of the native protein and also exhibited less secondary st ...	1997	9261944
dissection of pathways implicated in integrin-mediated actin cytoskeleton assembly. involvement of protein kinase c, rho gtpase, and tyrosine phosphorylation.	a panel of antibodies to the alphaiibbeta3 integrin was used to promote adhesion of chinese hamster ovary cells transfected with the alphaiibbeta3 fibrinogen receptor. while some alphaiibbeta3 antibodies were not able to induce p125 focal adhesion kinase (p125fak) tyrosine phosphorylation, all the antibodies equally support cell adhesion but not spreading and assembly of actin stress fibers. absence of stress fibers was also obtained by plating on antibodies directed to the hamster beta1 integri ...	1997	9268301
the antimalarial drug, chloroquine, interacts with lactate dehydrogenase from plasmodium falciparum.	we have previously shown that a radioiodinated photoreactive analogue of chloroquine, [125i]n-(4-(4-diethylamino-1-methylbutylamino)quinolin-6-yl) -4-azido-2-hydroxybenzamide ([125i]asa-q), specifically labels two proteins in plasmodium falciparum with apparent molecular weights (mr) of 42 and 33 kda (foley m, deady lw, ng k, cowman af, tilley l. j biol chem 1994:269:6955-6961). we now report the identification of the 33 kda protein. the 33 kda protein was purified from plasmodium falciparum usi ...	1997	9274881
molecular cloning of the complementary dna for an additional member of the family of aortic aneurysm antigenic proteins.	we have purified and partially sequenced a protein from the adventitia of the human aorta (aortic aneurysm antigenic protein 40 kda; aaap-40) that has homologies to bovine aortic microfibril-associated glycoprotein (magp-36). it is immunoreactive with immunoglobulin g (iggs) purified from the serum and aortic wall of patients with abdominal aortic aneurysms. aaap-40 and magp-36 have fibrinogen-like and vitronectin-like motifs. screening an expression library constructed from human aortic adventi ...	1997	9279320
characterization of two virulence proteins secreted by rabbit enteropathogenic escherichia coli, espa and espb, whose maximal expression is sensitive to host body temperature.	enteropathogenic escherichia coli (epec) and rabbit epec (rdec-1) cause unique histopathological features on intestinal mucosa, including attaching/effacing (a/e) lesions. due to the human specificity of epec, rdec-1 has been used as an animal model to study epec pathogenesis. at least two of the previously identified epec-secreted proteins, espa and espb, are required for triggering host epithelial signal transduction pathways, intimate adherence, and a/e lesions. however, the functions of thes ...	1997	9284118
characterization of two f-actin-binding and oligomerization sites in the cell-contact protein vinculin.	vinculin, a structural protein of animal cells, is critically involved in the assembly of microfilament/plasma membrane junctions at cell contacts. to understand its role in organizing the distal portions of microfilaments into specific, morphologically distinct structures at these sites in more detail, we characterized its interaction with filamentous actin and with itself by means of in vitro assays. using recombinant proteins comprising different parts of the vinculin tail fused to the maltos ...	1997	9288940
a sensitive immunoassay to detect the alpha-chemokine gro in rabbit blood and lung fluids.	gro-alpha, gro-beta and gro-gamma are closely related peptides that stimulate growth of tumor cells and activate leukocytes in acute inflammatory reactions. in order to study the biology of gro peptides in the lungs of experimental animals, we have developed and characterized a sensitive and specific immunoassay for rabbit gro, and used this assay to measure gro in rabbit lung fluids and plasma. gro was cloned from a rabbit cdna library and expressed in escherichia coli. specific goat polyclonal ...	1997	9294594
purification and characterization of the protein kinase encoded by the ul13 gene of herpes simplex virus type 2.	the proteins encoded by the ul13 genes of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) have been predicted to be protein kinases. to identify the ul13 gene product, we have raised a rabbit polyclonal antiserum against a his.tag-hsv-1 ul13 fusion protein. the antibody specifically reacted with the 60-kda ul13 fusion protein expressed in escherichia coli and also recognized 56- to 57-kda late proteins in nuclear fractions of hsv-1- and hsv-2-infected cells. on the other hand, novel casein ki ...	1997	9300039
critical role of lipid composition in membrane permeabilization by rabbit neutrophil defensins.	we have examined the interactions of the six known rabbit neutrophil defensin antimicrobial peptides with large unilamellar vesicles (luv) made from various lipid mixtures based on the lipid composition of escherichia coli membranes. we find that the permeabilization of luv made from e. coli whole lipid extracts differs dramatically from that of single-component luv made from palmitoyl-oleoyl-phosphatidylglycerol (popg). specifically, defensins np-1, np-2, np-3a, np-3b, and a natural mixture of ...	1997	9305875
mammalian protein rap46: an interaction partner and modulator of 70 kda heat shock proteins.	a ubiquitously expressed nuclear receptor-associating protein of approximately 46 kda (rap46) was identified recently. interaction experiments with in vitro-translated proteins and proteins contained in cell extracts revealed that a great variety of cellular regulators associate with rap46. however, in direct interaction tests by the far-western technique, only 70 kda proteins showed up and were identified as members of the 70 kda heat shock protein (hsp70) family. interaction is specific since ...	1997	9312007
rabbit ubiquitin-activating enzyme e1: cdna cloning, sequence and expression.	a cdna clone encoding ubiquitin-activating enzyme e1 has been isolated from a rabbit heart cdna library and sequenced. the 3.485 kb cdna contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kda. the deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. northern blot analysis reveals a single transcript of approx. 3.5 kb in all the rabbit tissues examined. the entire coding r ...	1997	9322736
expression of three functional domains of connexin 32 as thioredoxin fusion proteins in escherichia coli and generation of antibodies.	gap junctions, intercellular channels that allow cells to communicate directly, are constructed from connexin protein subunits. connexins traverse the membrane four times and have two extracellular loops and one intracellular loop; the amino and carboxyl tails are located at the intracellular aspect of the plasma membrane. the first extracellular domain (el1; residues 42-75), the intracellular domain (il; 94-130), the carboxyl-terminus (ct; 208-283), and the full-length rat connexin 32 (cx32) ge ...	1997	9325135
midgut-specific immune molecules are produced by the blood-sucking insect stomoxys calcitrans.	we have cloned and sequenced two defensins, smd1 and smd2, from anterior midgut tissue of the blood-sucking fly stomoxys calcitrans. the dna and n-terminal protein sequences suggest both are produced as prepropeptides. smd1 differs from the classic defensin pattern in having an unusual six-amino acid-long n-terminal sequence. both smd1 and smd2 have lower pi points and charge than insect defensins derived from fat body/hemocytes. northern analysis shows both of these defensin molecules are tissu ...	1997	9326639
colonic structural and ion transport abnormalities in suckling rabbits infected with escherichia coli k12.	escherichia coli k12 is a laboratory strain considered nonpathogenic. the purpose of this study was to examine the effect of e. coli k12 infection on colonic structure and function.	1997	9327369
[effects of human i.v. immunoglobulin on bacterial clearance and granulocyte function in endotoxinemia].	the therapeutic impact of intravenous immunoglobulins (ivig) in septic patients remains controversial. until now, the mechanisms of action have not been fully elucidated. since polymorphonuclear neutrophils (pmn) play a key role in host defence, this study focuses on the effects of ivig on bacterial clearance and pmn respiratory burst activity during endotoxinaemia. for this purpose, it was investigated whether ivig improves blood clearance and organ colonisation as well as pmn functions after e ...	1997	9340030
recognition of follicle stimulating hormone (alpha-subunit) by a recombinant receptor protein domain coded by an alternately spliced mrna and expressed in escherichia coli.	to assess the functional significance of putative proteins encoded by alternately spliced mrna of the sheep testicular fsh receptor, a short form cdna comprising of the first four exons (117 residues mature protein) was engineered for expression in escherichia coli. the expressed protein of molecular mass 15 kda was purified to homogeneity and verified by reaction with an antibody against a synthetic peptide sequence unique to the amino (n)-terminal region fsh receptor. the purified fsh receptor ...	1997	9343310
molecular cloning, sequencing, and expression in escherichia coli of mouse flavin-containing monooxygenase 3 (fmo3): comparison with the human isoform.	the sequence of mouse flavin-containing monooxygenase 3 (fmo3) was obtained from several clones isolated from a mouse liver cdna library. the nucleotide sequence of mouse fmo3 was 2020 bases in length containing 37 bases in the 5' flanking region, 1602 in the coding region, and 381 in the 3' flanking region. the derived protein sequence consisted of 534 amino acids including the putative flavin adenine dinucleotide and nadp+ pyrophosphate binding sites (characteristic of mammalian fmos) starting ...	1997	9344459
purification and characteristics of recombinant mouse metallothionein-i from escherichia coli.	the mouse metallothionein-i (mmt-i) cdna was amplified by polymerase chain reaction (pcr), inserted into vector pgex-4t-1, and expressed in escherichia coli as a carboxyl terminal extension of the 26-kda glutathione-s-transferase (gst). analyzed by sds-page, the amount of the expressed fusion protein gst-mt was over 50% of total cellular proteins. after the fusion protein had been digested with thrombin on a glutathione-sepharose 4b affinity chromatography column, recombinant mmt-i was purified ...	1997	9354383
a single amino acid substitution in ribonucleolytic toxin restrictocin abolishes its specific substrate recognition activity.	restrictocin is a small basic protein produced by the fungus aspergillus restrictus. it potently inhibits protein synthesis in eukaryotic cells by specifically cleaving a single phosphodiester bond in 28s rrna. a histidine residue at position 49 in restrictocin has been implicated in its active site. a mutant of restrictocin in which the histidine at position 49 was changed to an alanine was constructed by site-directed mutagenesis, and the protein was expressed in escherichia coli. the mutant a ...	1997	9354640
the product of the us10 gene of herpes simplex virus type 1 is a capsid/tegument-associated phosphoprotein which copurifies with the nuclear matrix.	we have identified the herpes simplex virus type 1 (hsv-1) us10 gene product using rabbit polyclonal antisera raised against a recombinant 6xhis-us10 fusion protein expressed in escherichia coli. the antiserum reacted specifically with 34 and 36 kda proteins in hsv-1 kos-infected cells as shown by western blotting and immunoprecipitation experiments. the 36 kda protein was immunoprecipitated with the us10 antiserum from 32p-labelled lysates of vero cells infected with hsv-1 kos, demonstrating th ...	1997	9367380
experimentally induced intrauterine infection causes fetal brain white matter lesions in rabbits.	periventricular leukomalacia, a common brain white matter lesion in preterm neonates, is a major risk factor for cerebral palsy. epidemiologic studies have demonstrated an association between infection and periventricular leukomalacia. the purpose of this study was to determine whether ascending intrauterine infection could cause brain white matter lesions in the fetal rabbit.	1997	9369822
glucose modulates hemodynamic, metabolic, and inflammatory responses to lipopolysaccharide in rabbits.	glucose is important for vascular and immunocompetent cell functions. we hypothesized that modifications in glucose metabolism (normal feeding, 24-h fasting, glucose loading) may influence the hemodynamic, metabolic, and inflammatory responses to lipopolysaccharide administration (lps; 600 micrograms/kg iv) in rabbits. aortic (abfv), hepatic artery (habfv), and portal vein blood flow velocities (pvbfv) (pulsed doppler), plasma tumor necrosis factor (tnf) and nitrites were measured. fasting deple ...	1997	9375321
[the detection and study on enterotoxigenic escherichia coli in china].	in this paper, some strains of etec were first detected from the stools of the patients with cholerae-like diarrhea in china in 1974. with a detected rate 36.48% of etec detected rates weve 20.18% from patients with acute diarrhea, 19.74% from environment and sea foods. etec produced most of lt toxin in culture medium when the they were incubated at 37-42 degrees c for 4 days. when rabbit ileal loop test (rilt) was performed with the culture of etec or with germ-free culture supernatant, a large ...	1996	9387570
[expression of human brain myelin basic protein gene cdna in escherichia coli].	a 612bp full-length cdna sequence coding for 21.5 kd myelin basic protein (mbp) was amplified from human brain cdna library by polymerase chain reaction, cloned into vector pgem-3zf(+) with blunt-end. the 561bp cloned fragment was recovered from recombinant pgemp after bamhi digestion, and then inserted into the bamhi site downstream of the glutathione-s-transferase gene of the expression vector pgex-5t. the recombinant vector p5tbp was transformed into e. coli jm 109 and positive colonies were ...	1996	9389051
expression of protein fragments from the human pkd1 gene and production of rabbit polyclonal antibodies to the recombinant proteins.		1997	9399038
effect of inhaled nitric oxide on endotoxin-induced hypoxaemia in rabbits.	in five mechanically ventilated rabbits, we studied the property of inhaled nitric oxide in helping to treat hypoxaemia which was induced by intravenous endotoxin (escherichia coli-derived lipopolysaccharide, serotype 0111: b4). we used measurements of arterial partial pressure of oxygen to check a therapeutic nitric oxide benefit. pulmonary artery pressure was continuously monitored. furthermore, we determined the single-breath diffusing capacity for nitric oxide. measurements of plasma nitrite ...	1997	9401583
transplantation of adenovirally transduced allogeneic chondrocytes into articular cartilage defects in vivo.	gene transfer to chondrocytes followed by intra-articular transplantation may allow for functional modulation of chondrocyte biology and enhanced repair of damaged articular cartilage. we chose to examine the loss of chondrocytes transduced with a recombinant adenovirus containing the gene for escherichia coli beta-galactosidase (ad.rsvntlacz), followed by transplantation into deep and shallow articular cartilage defects using new zealand white rabbits as an animal model. a type i collagen matri ...	1997	9404472
identification of a novel, non-snrnp protein complex containing u1a protein.	mouse monoclonal antibodies (mabs) were generated against escherichia coli-produced u1snrnp-a (u1a) protein. u1a-specific mabs as well as mabs that reacted with both u1a and u2snrnp-b" (u2b") were isolated. mab 12e12 was unique among the characterized mabs because it failed to immunoprecipitate u1a protein produced by in vitro transcription and translation using rabbit reticulocyte lysates. however, when u1a protein was made using a wheat germ extract, mab 12e12 could immunoprecipitate u1a quite ...	1997	9404895
enzymatic activity of 2'-5'-oligoadenylate synthetase is impaired by specific mutations that affect oligomerization of the protein.	previous studies from our laboratory have shown that deletion of residues 321 to 344 of the 9-2 isozyme of 2'-5'-oligoadenylate (2-5(a)) synthetase causes a loss of its enzyme activity (ghosh, s. k., kusari, j., bandyopadhyay, s. k., samanta, h., kumar, r., and sen, g. c. (1991) j. biol. chem. 266, 15293-15299). sequence comparison of this region among the different isozymes of 2-5(a) synthetases revealed that the residues at positions 330 to 333 are highly conserved. alanine-scanning mutagenesi ...	1997	9407111
recombinant subunits of mammalian elongation factor 1 expressed in escherichia coli. subunit interactions, elongation activity, and phosphorylation by protein kinase ckii.	the first step in elongation requires two different activities; elongation factor (ef)-1alpha transfers aminoacyl-trna to the ribosome and is released upon hydrolysis of gtp, ef-1betagammadelta catalyzes exchange of gdp on ef-1alpha with gtp. to analyze the role of the individual subunits of ef-1 in elongation, the cdnas for the beta, gamma, and delta subunits of ef-1 from rabbit were cloned, and proteins of 225, 437, and 280 amino acids, respectively, were expressed in escherichia coli. the pur ...	1997	9407120
isolation of a rat histidase cdna sequence and expression in escherichia coli--evidence of extrahepatic/epidermal distribution.	histidase (histidine ammonia-lyase) is a cytosolic enzyme responsible for catalyzing the non-oxidative deamination of histidine to urocanic acid. full-length cdnas encoding rat histidase have been isolated from a lambdazap liver cdna library using a partial cdna fragment obtained by pcr. whereas the initial description of the rat histidase 3' untranslated sequence contained a rare polyadenylation signal sequence, the data presented encompass a more distant 28-bp region, possessing a nucleotide s ...	1997	9432011
the myxoma virus m-t4 gene encodes a novel rdel-containing protein that is retained within the endoplasmic reticulum and is important for the productive infection of lymphocytes.	to investigate the contribution of the myxoma virus m-t4 gene to viral virulence, both copies of the m-t4 gene were inactivated by disruption and insertion of the escherichia coli guanosine phosphoribosyltransferase gene. infection of european rabbits with the recombinant m-t4-deleted virus, vmyxlact4, resulted in disease attenuation. in contrast, infection of rabbits with vmyxlac elicited the classical features of lethal myxomatosis. a notable decrease in the number of secondary lesions in anim ...	1997	9434727
intramolecular repression of mouse heat shock factor 1.	the pathway leading to transcriptional activation of heat shock genes involves a step of heat shock factor 1 (hsf1) trimerization required for high-affinity binding of this activator protein to heat shock elements (hses) in the promoters. previous studies have shown that in vivo the trimerization is negatively regulated at physiological temperatures by a mechanism that requires multiple hydrophobic heptad repeats (hrs) which may form a coiled coil in the monomer. to investigate the minimal requi ...	1998	9447987
endotoxin-induced disseminated intravascular coagulation in rabbits: effect of recombinant hirudin on hemostatic parameters, fibrin deposits, and mortality.	we evaluated the effect of r-hirudin on an experimental model of disseminated intravascular coagulation (dic) in rabbits, through the continuous infusion of 100 microg/kg/hr of escherichia coli endotoxin for a period of 6 hours. r-hirudin (0.05, 0.3, and 0.6 mg/kg/hr) as treatment, or saline solution as placebo, were administered simultaneously with endotoxin. severe dic in the endotoxin control group was shown by impairment in hemostatic parameters, kidney fibrin deposition, and a high mortalit ...	1998	9452130
towards a recombinant vaccine against diphtheria toxin.	two recombinant fragments of diphtheria toxin (dt) were fused to an engineered tandem repeat of the immunoglobulin (ig) binding domain of protein a, called zz. these fragments are (i) the receptor binding domain (dtr), which comprises amino acids 382 to 535 of dt, and (ii) a linear peptide (dt(168-220)) which comprises residues 168 to 220 of the loop between fragment a and fragment b of dt. the fusion proteins were produced in escherichia coli and purified by affinity chromatography. in vitro ex ...	1998	9453589
chicken antibodies to a recombinant fragment of the equine immunoglobulin epsilon heavy-chain recognising native horse ige.	an equine immunoglobulin e (ige) heavy-chain cdna fragment (ch3-ch4, nucleotides 1132 to 1592) was cloned, expressed in escherichia coli as a fusion protein with a [his]6-tag and purified over a ni-nta column. the recombinant protein was used to immunise hens. testing of the raised egg yolk immunoglobulin g (igg) in western-blot and elisa revealed high titres against the recombinant equine ige fragment (reqigef). the reqigef-specific igg was successfully affinity-purified on an unconventional af ...	1997	9477476
autonomous folding of the recombinant large cytoplasmic loop of sarcoplasmic reticulum ca2+-atpase probed by affinity labeling and trypsin digestion.	recombinant large cytoplasmic loop (lcl, residues 329-740) of sarcoplasmic reticulum ca2+-atpase, expressed in and purified from escherichia coli, comprises most of the active site and binds atp [moutin, m.-j., cuillel, m., rapin, c., miras, r., anger, m., lompré, a.-m. & dupont, y. (1994) j. biol. chem. 269, 11147-11154]. in this study, we show that fluorescein-5' isothiocyanate (fitc) specifically labels the same lysine residue as in the native ca2+-atpase (lys515), with similar kinetics and p ...	1998	9490041
heat-stress response of maize mitochondria.	we have identified maize (zea mays l. inbred b73) mitochondrial homologs of the escherichia coli molecular chaperones dnak (hsp70) and groel (cpn60) using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots. during heat stress (42 degrees c for 4 h), levels of hsp70 and cpn60 proteins did not change significantly. in contrast, levels of two 22-kd proteins increased dramatically (hsp22). monoclonal antibodies were developed to maize hsp70, cpn60, and hsp22. t ...	1998	9501143
the production of recombinant dengue virus e protein using escherichia coli and pichia pastoris.	the dengue virus envelope protein was expressed as a gst fusion protein using e. coli and p. pastoris as expression hosts. in e. coli the recombinant e protein is expressed initially as a soluble 81 kda gst fusion protein. treatment of the fusion protein with thrombin released a 55 kda protein, which is the expected size for correctly processed, non-glycosylated recombinant e protein. the antiserum from animals immunised with this recombinant e protein was found to specifically recognise the den ...	1997	9504761
recombinant production of cyanovirin-n, a potent human immunodeficiency virus-inactivating protein derived from a cultured cyanobacterium.	here we describe the recombinant production and purification of a novel anti-human immunodeficiency virus (hiv) protein, cyanovirin-n (cv-n), in escherichia coli. initial attempts to express cv-n using a vector containing an ompa signal peptide sequence resulted in production of an intractable mixture of the full-length (101 amino acid residue) protein and a truncated form lacking the first two n-terminal amino acids. the truncated protein was observed regardless of the host cell line, culture c ...	1998	9518455
diversity of hemagglutination phenotypes among p-fimbriated wild-type strains of escherichia coli in relation to papg allele repertoire.	data regarding the hemagglutination (ha) patterns of the three variants (classes i, ii, and iii) of the escherichia coli adhesin papg are conflicting. these ha patterns usually have been assessed for each papg allele separately with recombinant strains in slide ha assays. we rigorously evaluated an alternative microtiter tray ha assay and then used it to assess the ha of four erythrocyte types (human a1p1 and op1, rabbit, and sheep erythrocytes) by multiple wild-type e. coli strains representing ...	1998	9521137
sequence analysis of the mip gene of the soilborne pathogen legionella longbeachae.	to understand the basis of pathogenesis by legionella longbeachae serogroup 1, the importance of the mip protein in this species was examined. amino-terminal analysis of the purified, cloned l. longbeachae serogroup 1 atcc 33462 mip protein confirmed that the cloned gene protein was expressed and processed in an escherichia coli background. dna sequence analysis of plasmid pimvs27, containing the entire l. longbeachae serogroup 1 mip gene, revealed a high degree of homology to the mip gene of le ...	1998	9529072
characterization of v3 loop-pseudomonas exotoxin chimeras. candidate vaccines for human immunodeficiency virus-1.	to develop a candidate vaccine for human immunodeficiency virus, type 1 (hiv-1), chimeric proteins were constructed by inserting sequences derived from the v3 loop of gp120 into a nontoxic form of pseudomonas exotoxin (pe). inserts of 14 or 26 amino acids, constrained by a disulfide bond, were introduced between domains ii and iii of pe. v3 loop-toxin proteins expressed in escherichia coli and corresponding to either mn (subtype b) or thai (subtype e) strains, were recognized by strain-specific ...	1998	9545339
expression of recombinant antigens in escherichia coli: application on immunochemical studies of schistosoma mansoni tegumental antigens.	sm15 and sm13 are recognized by antibodies from mice protectively vaccinated with tegumental membranes, suggesting a potential role in protective immunity. in order to raise antibodies for immunochemical investigations, the genes for these antigens were expressed in pgex and pmal vectors so that comparisons could be made among different expression systems and different genes. the fusion proteins corresponding to several parts of the gene for the precursor of sm15 failed in producing antibodies r ...	1997	9566231
expression of membrane-associated proteins by strains of enteroaggregative escherichia coli.	certain strains of enteroaggregative escherichia coli express an outer membrane-associated protein, involved with the adhesion of these bacteria to hep-2 cells. strains of enteroaggregative e. coli hybridising with dna probes for aggregative adhesion, diffuse adhesion and aggregative adhesion fimbriac ii expressed an outer membrane-associated protein of 18 kda regulated by magnesium ions. strains hybridising with the aggregative adhesion probe only expressed a 20-kda outer membrane-associated pr ...	1998	9570123
rainbow trout glucocorticoid receptor overexpression in escherichia coli: production of antibodies for western blotting and immunohistochemistry.	fragments of cdna that encode the n-terminal and dna-binding domains (dbd) of the rainbow trout glucocorticoid receptor (rtgr) were expressed in escherichia coli as fusion proteins with glutathione-s-transferase (gst). the fusion proteins induced by iptg could readily be detected as 45- and 40-kda bands, respectively, in crude extracts, as well as in proteins purified on glutathione-agarose. these purified hybrid proteins were used to immunize rabbits. the antisera produced were tested for speci ...	1998	9570941
characterization of the roles of hemolysin and other toxins in enteropathy caused by alpha-hemolytic escherichia coli linked to human diarrhea.	escherichia coli strains producing alpha-hemolysin have been associated with diarrhea in several studies, but it has not been clearly demonstrated that these strains are enteropathogens or that alpha-hemolysin is an enteric virulence factor. such strains are generally regarded as avirulent commensals. we examined a collection of diarrhea-associated hemolytic e. coli (dhec) strains for virulence factors. no strain produced classic enterotoxins, but they all produced an alpha-hemolysin that was in ...	1998	9573087
molecular cloning and expression in escherichia coli of cdna encoding bonnet monkey (macaca radiata) zona pellucida glycoprotein-zp2.	zona pellucida (zp) glycoproteins have been proposed as candidate antigens for an immunocontraceptive vaccine. the efficacy of such a vaccine has to be evaluated in nonhuman primates, thus necessitating the characterization of their zp glycoproteins. a bonnet monkey (macaca radiata) ovarian cdna lambda gt11 library was screened for zp2 (bzp2) using full-length human zp2 cdna as a probe. two identical full-length clones with an open reading frame of 2235 nt encoding a polypeptide of 745 aa residu ...	1998	9590540
[the characteristics of the changes in the vascular endothelium--the main target of the action of lipopolysaccharide in endotoxic shock].		1997	9591342