Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the retinoic acid receptor-beta 2 contains two separate cell-specific transactivation domains, at the n-terminus and in the ligand-binding domain. | in contrast to other members of the steroid/thyroid hormone superfamily, not much is known about the regions involved in transactivation of the receptors for retinoic acid. to determine the transactivation function of rars, fusion proteins between the dna-binding domain of the yeast transcription factor gal4 and retinoic acid receptor-alpha (rar alpha) or rar beta were made. transfection of these constructs resulted in ra-induced activation of a gal4-responsive element-containing promoter. delet ... | 1993 | 8389001 |
| mutation of the conserved first nucleotide of a group ii intron from yeast mitochondrial dna reduces the rate but allows accurate splicing. | group ii introns have a phylogenetically conserved 5'-terminal pentanucleotide, -g1u2g3c4g5-, that resembles the conserved 5' end sequence of nuclear pre-mrna introns. no functional interaction or catalytic role for the conserved g1 position has been proposed, although a tertiary structure involving -g3c4- has been implicated in splicing in vitro. we have analyzed splicing phenotypes both in vitro and in vivo for all three point mutants affecting guanosine at position 1 (g1) of intron 5 gamma fr ... | 1993 | 8389367 |
| use of yeast in the study of anticancer drugs targeting dna topoisomerases: expression of a functional recombinant human dna topoisomerase ii alpha in yeast. | a plasmid was constructed for the expression of human dna topoisomerase ii alpha in yeast from a galactose-inducible promoter of the yeast gal1 gene. expression of a recombinant human enzyme, in which the first 28 of the 1531 codons of human dna topoisomerase ii alpha were replaced by the first five codons of yeast dna topoisomerase ii, was shown to rescue the lethal phenotype of thermal sensitive yeast dna topoisomerase ii mutants at 35 degrees c. purification of the human enzyme overexpressed ... | 1993 | 8393377 |
| biochemical analysis of mutant alleles of the vaccinia virus topoisomerase i carrying targeted substitutions in a highly conserved domain. | the 32-kda topoisomerase i encoded by vaccinia virus relaxes supercoiled dna in a manner which is mechanistically equivalent to that utilized by eucaryotic enzymes. its amino acid sequence contains significant homology to the enzymes encoded by saccharomyces cerevisiae, saccharomyces pombe, human cells, and other poxviruses. the small size of the viral enzyme, and its essentiality in the viral life cycle, make it ideally suited for structural and functional analysis. in this report we present th ... | 1993 | 8393454 |
| mapping a putative pyruvoyl decarboxylase active site to human cytomegalovirus open reading frame ul77. | human cytomegalovirus is present as an apparently innocuous infection among a large proportion of the adult population that causes serious disease when congenitally transferred (1). human cytomegalovirus disease may become life threatening when found as an infection of immunocompromised transplant and aids patients (1,2). we now map a putative pyruvoyl decarboxylase enzyme prosthetic group, known to be essential to the active site of this class of enzymes, to human cytomegalovirus open reading f ... | 1993 | 8394702 |
| camptothecin resistance from a single mutation changing glycine 363 of human dna topoisomerase i to cysteine. | a full-length human dna topoisomerase i complementary dna clone was mutagenized in vitro and the mutagenized dna was used to replace wild-type human top1 complementary dna in ycpgal1-htop1, a plasmid constructed for the expression of the human enzyme in yeast. a yeast strain devoid of yeast dna topoisomerase i and permeable to the anticancer drug camptothecin was transformed with the plasmid pool. assays of dna topoisomerase i in lysates of camptothecin-resistant transformants identified one wit ... | 1993 | 8395982 |
| analysis of glyoxalase-i from normal and tumor tissue from human colon. | glyoxalase-i (gly-i) is part of the glyoxalase system which converts methylglyoxal to d-lactic acid via an s-d-lactoylglutathione intermediate. this glutathione (gsh)-binding protein was purified from human colon tumors and corresponding normal tissue. the gsh-affinity purified fraction from normal human colon tissue showed enzyme activity of 30.6 +/- 11.5 mumol/min per mg protein, with methylglyoxal as substrate. corresponding fractions from carcinomas showed significantly elevated gly-i activi ... | 1993 | 8399366 |
| the leading role of stss in genome mapping. | 1993 | 8401489 | |
| relationship between charcot-marie-tooth 1a and smith-magenis regions. snu3 may be a candidate gene for the smith-magenis syndrome. | the juxtacentromeric region of the human chromosome 17 short arm (17p11.2-p12) contains genes involved in the charcot-marie-tooth type 1a disease (cmt1a) and the smith-magenis syndrome (sms). cmt1a is associated with a duplication of a short segment whereas sms is linked to microdeletions, extending toward the centromere. we describe the construction and analysis of a 5 mb yac contig spanning the cmt1a duplicated segment and the distal part of four sms microdeletions. we concluded that the yac c ... | 1993 | 8401506 |
| report and abstracts of the fourth international workshop on human x chromosome mapping 1993. st. louis, missouri, may 9-12, 1993. | 1993 | 8404034 | |
| tandem linkage of genes coding for leukemia inhibitory factor (lif) and oncostatin m (osm) on human chromosome 22. | leukemia-inhibitory factor (lif) and oncostatin m (osm) are members of a family of structurally similar growth factors presenting overlapping and specific functions. although the genes coding for il-6, csf3 and cntf are scattered in the human and mouse genome, human lif and osm genes have conserved synteny in the course of evolution. through isolation of a yac and a cosmid clone containing both lif and osm we demonstrate that the two genes are linked in tandem on human chromosome 22q12, separate ... | 1993 | 8404048 |
| effect of mannose-binding protein on binding of cryptococcus neoformans to human phagocytes. | the capacity of human peripheral blood monocytes, neutrophils, and monocyte-derived macrophages to bind the yeast cryptococcus neoformans was increased when the phagocytes were cultured on surfaces containing recombinant human mannose-binding protein. in contrast, soluble mannose-binding protein had no effect on cryptococcal binding by phagocytes. | 1993 | 8406891 |
| a novel cdc2-related protein kinase from leishmania mexicana, lmmcrk1, is post-translationally regulated during the life cycle. | the p34cdc2 protein kinase is a key component in the regulation of the eukaryotic cell cycle. we have isolated from the protozoan parasite leishmania mexicana mexicana a cdc2-related kinase gene (lmmcrk1) encoding a 34-kda protein kinase (lmmcrk1) which has 56% amino acid identity with the human cdc2 and contains a pctair motif in place of the highly conserved pstair box. lmmcrk1 was detected in all life cycle stages at comparable levels, yet its histone h1 kinase activity was detected in only t ... | 1993 | 8407941 |
| use of a general purpose mammalian expression vector for studying intracellular protein targeting: identification of critical residues in the nuclear lamin a/c nuclear localization signal. | we have constructed a general purpose mammalian expression vector for the study of intracellular protein targeting. the vector, p3pk, facilitates construction of n- and/or c-terminal fusions of an amino acid sequence of interest to the normally cytosolic protein chicken muscle pyruvate kinase (cmpk). the vector has been engineered such that any fusion construct can be subcloned into the versatile pjx omega family of mammalian expression vectors and into pgex bacterial expression vectors, for the ... | 1993 | 8408279 |
| transcriptional trans activators of human and simian foamy viruses contain a small, highly conserved activation domain. | the bel-1 protein of human foamy virus is a potent transcriptional trans activator of its homologous long terminal repeat promoter element. here, we demonstrate that bel-1 can also efficiently activate gene expression when targeted to a heterologous promoter by fusion to the dna-binding motif of the yeast gal4 protein. analysis of a series of deletion mutants of bel-1 generated in this hybrid protein context suggests the presence of a single transcription activation domain that is fully containe ... | 1993 | 8411385 |
| [structural analysis of human genome by yac technologies]. | a method for construction of yac (yeast artificial chromosome) libraries with large inserts has been developed and promoted the ongoing project of human genome. isolation by pcr screening charges the yac clone with a unique tag of a pair of pcr primers at the defined chromosome site (sequence tagged sites; sts). current evaluation of yac has revealed that larger yac has more problems where rearrangements including deletion and chimera occur extensively in dna molecules, presenting a limited use ... | 1993 | 8411697 |
| a family of human phosphodiesterases homologous to the dunce learning and memory gene product of drosophila melanogaster are potential targets for antidepressant drugs. | we have isolated cdnas for four human genes (dpde1 through dpde4) closely related to the dnc learning and memory locus of drosophila melanogaster. the deduced amino acid sequences of the drosophila and human proteins have considerable homology, extending beyond the putative catalytic region to include two novel, highly conserved, upstream conserved regions (ucr1 and ucr2). the upstream conserved regions are located in the amino-terminal regions of the proteins and appear to be unique to these ge ... | 1993 | 8413254 |
| human xeroderma pigmentosum group d gene encodes a dna helicase. | xeroderma pigmentosum (xp), a genetically heterogeneous human disease, results from a defect in nucleotide excision repair of ultraviolet-damaged dna. xp patients are extremely sensitive to sunlight and suffer from a high incidence of skin cancers. cell fusion studies have identified seven xp complementation groups, a-g. group d is of particular interest as mutations in this gene can also cause cockayne's syndrome and trichothiodystrophy. the xpd gene was initially named ercc2 (excision repair c ... | 1993 | 8413672 |
| a cdna sequence of human ribosomal protein, homologue of yeast s28. | 1993 | 8415000 | |
| the yeast candida albicans has a clonal mode of reproduction in a population of infected human immunodeficiency virus-positive patients. | to ascertain the population structure of candida albicans, we have carried out a multilocus enzyme electrophoresis study based on the analysis of 21 gene loci. we have thus characterized 55 strains isolated one each from 55 human immunodeficiency virus-positive patients. there is considerable polymorphism among the strains. a population-genetic analysis indicates that the two fundamental consequences of sexual reproduction (i.e., segregation and recombination) are apparently absent in this popul ... | 1993 | 8415722 |
| estradiol-inducible squelching and cell growth arrest by a chimeric vp16-estrogen receptor expressed in saccharomyces cerevisiae: suppression by an allele of pdr1. | we have constructed and characterized a flexible system for analyzing the phenomenon of squelching and estrogen receptor function in the yeast saccharomyces cerevisiae. the a/b region of the human estrogen receptor was replaced with the transcriptional activating domain of vp16 and expressed in yeast cells from high-copy-number plasmids. addition of hormone resulted in an immediate inhibition of expression (squelching) of a chromosomally integrated gal1:lacz reporter gene and the eventual arrest ... | 1993 | 8417344 |
| interferon-gamma activates the oxidative killing of candida albicans by human granulocytes. | although granulocytes are essential for the resistance against infections with candida albicans, these cells do not kill the ingested yeast optimally. various cytokines can enhance functional activities of granulocytes, but until now only interferon-gamma (ifn-gamma) has been applied more widely, namely in patients with chronic granulomatous disease. since it is not certain whether ifn-gamma is able to enhance the candidacidal activity of granulocytes the present study was undertaken. human gran ... | 1993 | 8419079 |
| the type 1 human immunodeficiency virus tat binding protein is a transcriptional activator belonging to an additional family of evolutionarily conserved genes. | the type 1 human immunodeficiency virus tat protein is a powerful transcriptional activator when bound to an rna structure (tar) present at the extreme 5' terminus of viral mrna. since transcriptional activation requires binding of tat to rna, it has been suggested that tat enhances initiation or elongation through a direct interaction with cellular transcription factors. here we show through protein fusion experiments that the previously identified cellular tat binding protein, tbp-1, although ... | 1993 | 8419915 |
| structure and function of eukaryotic proprotein processing enzymes of the subtilisin family of serine proteases. | production of a broad spectrum of regulatory proteins in eukaryotes occurs via an intricate cascade of biosynthetic and secretory processes. often these proteins initially are synthesized as parts of higher molecular weight, but inactive, precursor proteins. specific endoproteolytic processing of these proproteins is required to generate the regulatory proteins in a mature and biologically active form. such endoproteolysis generally occurs at cleavage sites consisting of particular sequence moti ... | 1993 | 8420571 |
| identification and characterization of the cytochrome p450 enzymes involved in n-dealkylation of propafenone: molecular base for interaction potential and variable disposition of active metabolites. | the activity of metabolizing enzymes determines plasma concentrations and hence effects of drugs. identification of these enzymes may allow the prediction of both the interaction potential of drugs and the variability deriving from certain pathways. the antiarrhythmic propafenone is extensively biotransformed to the active metabolites 5-hydroxypropafenone and n-desalkylpropafenone. whereas 5-hydroxylation is catalyzed by cyp2d6, the enzyme involved in n-dealkylation has not been identified. we, ... | 1993 | 8423765 |
| heat shock factor is required for growth at normal temperatures in the fission yeast schizosaccharomyces pombe. | schizosaccharomyces pombe is becoming an increasingly useful organism for the study of cellular processes, since in certain respects, such as the cell cycle and splicing, it is similar to metazoans. previous biochemical studies have shown that the dna binding ability of s. pombe heat shock factor (hsf) is fully induced only under stressed conditions, in a manner similar to that of drosophila melanogaster and humans but differing from the constitutive binding by hsf in the budding yeasts. we repo ... | 1993 | 8423799 |
| a new transcriptional-activation motif restricted to a class of helix-loop-helix proteins is functionally conserved in both yeast and mammalian cells. | previous studies demonstrated that the amino-terminal portions of e2a and e2-2 are crucial for transactivation. subsequent findings showed that the same amino-terminal region of e2a is involved in two different translocation events contributing to the induction of a pre-b-cell acute lymphoblastic leukemia and a pro-b-cell acute lymphoblastic leukemia. these results led us to focus on the amino-terminal region of e2a to better understand its normal role in transcriptional regulation and its aberr ... | 1993 | 8423802 |
| synthesis of antimony complexes of yeast mannan and mannan derivatives and their effect on leishmania-infected macrophages. | antimony(sb)-yeast mannan complexes were synthesized as a strategy to introduce sb into macrophages infected with leishmania amastigotes. the complexes were taken up by endocytosis after specific recognition by alpha-d-mannosyl receptors on the macrophage membrane. about 90% of the intracellular parasites were destroyed by sb-mannan in vitro, whereas the corresponding sb concentration used as the pentavalent antimonial drug glucantime destroyed about 60% of the amastigotes. none of the sb comple ... | 1993 | 8424752 |
| mxi1, a protein that specifically interacts with max to bind myc-max recognition sites. | we used the interaction trap to isolate a novel human protein that specifically interacts with max. this protein, mxi1 (for max interactor 1), contains a bhlh-zip motif that is similar to that found in myc family proteins. mxi1 interacts specifically with max to form heterodimers that efficiently bind to the myc-max consensus recognition site. when bound to dna by a lexa moiety in yeast, mxi1 does not stimulate transcription. mxi1 mrna is expressed in many tissues, and its expression is elevated ... | 1993 | 8425219 |
| cloning, sequencing and expression of a cdna encoding mammalian valyl-trna synthetase. | a fragment of the cdna encoding a rat valyl-trna synthetase (trsval)-like protein was cloned from a rat cdna library in lambda gt11 using an oligodeoxyribonucleotide (oligo) probe. three independent plaque clones containing the human trsval cdna were then isolated from a lambda gt10 human erythroleukemia cdna library using the rat cdna fragment as the hybridization probe. sequence analyses of the cdna fragments provided a 3.2-kb sequence with an open reading frame that contained the 'high' synth ... | 1993 | 8428657 |
| recombinant human sickle hemoglobin expressed in yeast. | sickle hemoglobin has been expressed in the yeast saccharomyces cerevisiae after site-directed mutagenesis of a plasmid containing normal human alpha- and beta-globin genes. cassette mutagenesis of this plasmid was achieved by inserting a dna fragment containing the beta-globin gene in the replicative form of m13mp18 to make a point mutation and then reconstituting the original plasmid containing the mutated beta-globin gene. pure recombinant hemoglobin s was shown to be identical to natural sic ... | 1993 | 8430105 |
| cloning and characterization of eagi yacs from human chromosome 21. | yeast artificial chromosomes (yacs) were made from a total eagi digest of dna from a mouse-human chromosome 21 hybrid cell line. approximately 3750 yacs, corresponding to 75-125 human yacs, with an average size of approximately 100 kb were recovered. southern hybridization indicates that the chimera frequency in this library may be less than 3%. thirty-four of the human eagi yacs were regionally assigned by a number of methods. some yacs were regionally assigned to one of six chromosome regions ... | 1993 | 8432544 |
| purification and characterization of the trefoil peptide human spasmolytic polypeptide (hsp) produced in yeast. | recombinant human spasmolytic polypeptide (r-hsp) has been produced in relatively large amounts in saccharomyces cerevisiae. the two intronless trefoil domains of the hsp-dna were cloned separately by pcr from human genomic dna, and the remaining parts of the gene synthesized. recombinant plasmids were constructed to encode a fusion protein consisting of a hybrid leader sequence and the hsp sequence. the leader sequence serves to direct the fusion protein into the secretory pathway of the cell a ... | 1993 | 8440393 |
| influence of guanine nucleotides on complex formation between ras and cdc25 proteins. | the saccharomyces cerevisiae cdc25 gene and closely homologous genes in other eukaryotes encode guanine nucleotide exchange factors for ras proteins. we have determined the minimal region of the budding yeast cdc25 gene capable of activity in vivo. the region required for full biological activity is approximately 450 residues and contains two segments homologous to other proteins: one found in both ras-specific exchange factors and the more distant bud5 and lte1 proteins, and a smaller segment o ... | 1993 | 8441380 |
| a human mitochondrial transcriptional activator can functionally replace a yeast mitochondrial hmg-box protein both in vivo and in vitro. | human mitochondrial transcription factor a is a 25-kda protein that binds immediately upstream of the two major mitochondrial promoters, thereby leading to correct and efficient initiation of transcription. although the nature of yeast mitochondrial promoters is significantly different from that of human promoters, a potential functional homolog of the human transcriptional activator protein has been previously identified in yeast mitochondria. the importance of the yeast protein in yeast mitoch ... | 1993 | 8441424 |
| extension of yeast artificial chromosomes by cosmid multimers. | 1993 | 8441698 | |
| human catalase is imported and assembled in peroxisomes of saccharomyces cerevisiae. | to study the conservation of peroxisomal targeting signals, we have determined the intracellular localization of human peroxisomal catalase when expressed in yeast. using immunofluorescence, differential centrifugation and immunoelectron microscopy, we show that the protein is targeted to the peroxisomes of the heterologous cell and assembled in its active tetrameric form. these data show the conservation of the catalase targeting signal and import specificity between human and yeast peroxisomes ... | 1993 | 8442388 |
| pcr buffer optimization with uniform temperature regimen to facilitate automation. | to facilitate pcr(1,2) reactions in large numbers with uniform conditions, the annealing temperature was fixed and the stringency of the reactions was adjusted by optimizing the ion conditions of the reaction. the buffer system is based primarily on tris (t), ammonium (n), and potassium (k) to adapt assay conditions to different primer pairs. the tnk buffers have permitted successful screening of a 60,000-clone yeast artificial chromosome (yac) library with more than 200 primer pairs. | 1993 | 8443576 |
| characterisation of the gene encoding an unusually divergent tata-binding protein (tbp) from the extremely a+t-rich human malaria parasite plasmodium falciparum. | the intergenic regions of the human malaria parasite, plasmodium falciparum, are extreme in their base composition, averaging approx. 90% a + t. as a first step to investigating whether transcription in this organism follows conventional models based largely on yeast, we have isolated and characterised the gene (tbp) encoding its tata-binding protein (tbp). the gene is present as a single copy on chromosome 5 and is expressed as a 1.8-kb mrna encoding a protein of 228 amino acids (aa) (26 164 da ... | 1993 | 8444340 |
| functional expression of human cathepsin s in saccharomyces cerevisiae. purification and characterization of the recombinant enzyme. | a cdna encoding the human lysosomal cysteine proteinase cathepsin s precursor has been expressed in yeast using the pvt100-u expression vector containing the alpha-factor promoter. the procathepsin s gene was expressed either as a fusion protein with the pre-region or with the prepro-region of the yeast alpha-factor precursor gene. following in vitro processing both constructs gave an identical active mature enzyme with a molecular weight of 24,000. after prolonged cultivation of the cells the r ... | 1993 | 8444861 |
| comparison of the acyl chain specificities of human myristoyl-coa synthetase and human myristoyl-coa:protein n-myristoyltransferase. | human myristoyl-coa synthetase and myristoyl-coa:protein n-myristoyltransferase (hnmt) have been partially purified from an erythroleukemia cell line. their substrate specificities were examined using two in vitro assays of enzyme activity together with a panel of c7-c17 saturated fatty acids plus 72 myristic acid analogs containing oxygen, sulfur, ketocarbonyl, ester, amide, cis and trans double bonds, triple bonds, and para-substituted phenyl groups. there is an inverse relationship between th ... | 1993 | 8444867 |
| amplification of n-myc as a prognostic marker for patients with neuroblastoma. | n-myc is amplified between 5 and several hundred-fold frequently in neuroblastomas and, at lower frequency, in other human cancers with neuronal qualities, including retinoblastomas, gliomas, astrocytomas and small cell lung cancers. in neuroblastomas n-myc amplification is significantly correlated with poor prognosis; for other types of tumors such a correlation is difficult to make, due to low incidence of amplification. amplification is associated with elevated expression, both of mrna and pr ... | 1993 | 8448374 |
| enhanced in vivo monooxygenase activities of mammalian p450s in engineered yeast cells producing high levels of nadph-p450 reductase and human cytochrome b5. | we have engineered yeast genomic dna to construct a set of strains producing various relative amounts of yeast nadph-p450 reductase (yred) and human cytochrome b5 (hb5). expression of cdnas encoding human p450 1a1, 1a2, 3a4, 19a and mouse p450 1a1 in the different oxido-reduction backgrounds thus constituted were achieved after strain transformation by plasmid-based p450-encoding expression cassettes. the results indicate that the level of yred strongly affects all activities tested. in contrast ... | 1993 | 8449412 |
| comparative mapping on the mouse and human x chromosomes of a human cdna clone encoding the vasopressin renal-type receptor (avp2r). | mutations in the gene for the human renal-type vasopressin receptor (v2r) have recently been identified in patients with nephrogenic diabetes insipidus (ndi). both v2r and ndi have been independently mapped to xq28. using a combination of genetic and physical mapping, we have localized the murine v2r locus to within 100 kb of l1cam on the mouse x chromosome in a region syntenic with human xq28. based on conserved gene order of mouse and human loci in this region, physical mapping using dna deriv ... | 1993 | 8449515 |
| generation of ordered phage sublibraries of yac clones: construction of a 400-kb phage contig in the human dystrophin gene. | a phage contig of 400 kb that extends from the brain-specific promoter at the 5'-end of the human dystrophin gene, through the muscle-specific promoter over 100 kb further downstream, and across most of intron 1 has been assembled. to achieve this, a yeast artificial chromosome (yac) subcloning approach was used. total dna from a yeast strain containing a 400-kb yac from the dystrophin gene was cloned using a lambda phage vector containing rna polymerase promoters flanking the cloning sites. pha ... | 1993 | 8449519 |
| filamentous growth and elevated vaginopathic potential of a nongerminative variant of candida albicans expressing low virulence in systemic infection. | the vaginopathic potential and the intravaginal morphology of a nongerminative variant of candida albicans, strain ca-2, were studied in a rat vaginitis model. although it expressed low virulence in systemic infections, strain ca-2 was capable of causing a vaginal infection of the same duration and extent as that obtained in rats challenged with the germ-tube-forming strain c. albicans 3153 from the stock collection or with a fresh clinical isolate of c. albicans from a case of human vaginitis. ... | 1993 | 8454356 |
| molecular cloning of a rho family gene of entamoeba histolytica. | an entamoeba histolytica gene (eh rho1) was cloned that encodes a putative low-molecular-mass gtp-binding protein, most similar to the ras homologue rho. the eh rho1 open reading frame was 208 amino acids long and encoded a 23-kda protein similar to saccharomyces cerevisiae rho1-rho4 and cdc42 and human rhoa, rac1, and g25k gene products. this similarity was greatest at the nh2 terminus of eh rho1 where two gtp-binding sites and a possible effector site were conserved. a cysteine residue at the ... | 1993 | 8459832 |
| effect of myristoylation on gtp-dependent binding of adp-ribosylation factor to golgi. | adp-ribosylation factors (arfs), a family of approximately 20-kda guanine nucleotide-binding proteins that activate cholera toxin adp-ribosyltransferase in vitro, have been implicated in intracellular protein trafficking and are thought to cycle between cytosolic and membrane compartments. although isolated predominantly as soluble proteins, arfs associate with membranes and phospholipids in a gtp-dependent manner. in contrast to other small gtp-binding proteins, arfs are nh2 terminally myristoy ... | 1993 | 8463239 |
| prokaryotic members of a new family of putative helicases with similarity to transcription activator snf2. | cloning and sequence analysis of a new open reading frame from bacillus cereus reveals the relationship to a recently identified family of putative eukaryotic transcription activators similar to the yeast snf2 gene product. as a result of comparative analysis of sequence features conserved in all members of this family, a gene from a chilo iridescent virus, as well as a putative helicase from escherichia coli (hepa), can also be grouped into this family. the unexpected presence of prokaryotic an ... | 1993 | 8464078 |
| duplicated kox zinc finger gene clusters flank the centromere of human chromosome 10: evidence for a pericentric inversion during primate evolution. | two related zinc finger (znf) gene clusters from the pericentromeric region of human chromosome 10, defined by cdnas of the kox series, have been cloned in yeast artificial chromosomes (yacs). the two clusters evolved by duplication of an ancestral gene cluster before the divergence of the human and great ape lineages. included in cluster a are the znf gene sequences znf11a, znf33a, and znf37a, while cluster b comprises the related sequences znf11b, znf33b and znf37b. genes from both clusters ar ... | 1993 | 8464732 |
| clustered organization of homologous krab zinc-finger genes with enhanced expression in human t lymphoid cells. | krab zinc-finger proteins (krab-zfps) constitute a large subfamily of zfps of the krüppel c2h2 type. krab (krüppel-associated box) is an evolutionarily conserved protein domain found n-terminally with respect to the finger repeats. we report here the characterization of a particular subgroup of highly related human krab-zfps. znf91 is one representative of this subgroup and contains 35 contiguous finger repeats at its c-terminus. three mrna isoforms with sequence identity to znf91 were isolated ... | 1993 | 8467795 |
| a set of sts assays targeting the chromosome 22 physical framework markers. | the widespread use of the sequence-tagged site (sts) as a quick, efficient, and reproducible assay for comparing physical and genetic map information promises to facilitate greatly long-range goals of the mapping of the human genome. we have designed 21 sts assays for loci on human chromosome 22. these assays primarily tag the physical framework markers of the long arm of 22, but additional assays have been designed from known genes and loci in the neurofibromatosis 2 (nf2) region. the availabil ... | 1993 | 8468063 |
| human hepatic cyp1a1 and cyp1a2 content, determined with specific anti-peptide antibodies, correlates with the mutagenic activation of phip. | mono-specific antibodies targeted to human cyp1a1 and cyp1a2 have been produced by immunizing rabbits with protein conjugates of short synthetic peptides corresponding to residues 290-297 and 284-296 respectively, of these enzymes. the antibody targeted to cyp1a1 bound in immunoblotting to the recombinant protein expressed in yeast but did not bind to any human hepatic microsomal protein, whereas the antibody targeted to cyp1a2 bound only to this enzyme in immunoblotting of human hepatic microso ... | 1993 | 8472319 |
| treatment and serological studies of an italian case of penicilliosis marneffei contracted in thailand by a drug addict infected with the human immunodeficiency virus. | a case of disseminated penicilliosis marneffei, the first to be diagnosed in italy, is described in a male hiv-positive drug addict. the patient had visited thailand several times in the two years prior to his hospitalization. the presenting signs were fever, productive cough, facial skin papules and pustules, nodules on both thumbs and oropharyngeal candidiasis. penicillium marneffei was isolated from a series of blood specimens with the lysis centrifugation procedure. septate, yeast-like cells ... | 1993 | 8472804 |
| dominant negative mutants of human t-cell leukemia virus type i rex and human immunodeficiency virus type 1 rev fail to multimerize in vivo. | human t-cell leukemia virus type i (htlv-i) rex and human immunodeficiency virus type 1 (hiv-1) rev are essential gene products required for the replication of these two pathogenic human retroviruses. both rex and rev act at a posttranscriptional level by binding to highly structured rna-response elements, the rex-response element in htlv-i and the rev-response element in hiv-1. using a sensitive in vivo assay of protein-protein interaction, we now demonstrate that the htlv-i rex and hiv-1 rev p ... | 1993 | 8474155 |
| characterization of yeast ef-1 alpha: non-conservation of post-translational modifications. | elongation factor 1 alpha (ef-1 alpha) is an abundant cellular protein and its amino-acid sequence has been inferred from numerous organisms, including bacteria, archaebacteria, plants and animals. in large measure, it would appear that the overall structure has probably been maintained given the 33% identity and 56% similarity of escherichia coli ef-tu with human ef-1 alpha. chemical sequencing of ef-tu and ef-1 alpha has revealed that these proteins are post-translationally modified. in order ... | 1993 | 8476932 |
| structure of the sequences adjacent to the centromeric alphoid satellite dna array on the human y chromosome. | eighteen yeast artificial chromosome (yac) clones containing alphoid satellite dna and adjacent sequences from the human y chromosome have been identified from three different yac libraries. restriction site mapping of the genomic alphoid arrays and the yacs has allowed seven of the alphoid clones to be positioned on the arrays. three clones extend into flanking sequences. at one edge the alphoid dna is highly diverged and is flanked by a small block of the 48 base-pair satellite, dispersed mode ... | 1993 | 8478934 |
| transmembrane signaling pathways involved in phagocytosis and associated activation of nadph oxidase mediated by fc gamma rs in human neutrophils. | we have previously shown that in neutrophils classical transmembrane signaling consisting of increased [ca2+]i and hydrolysis of phospholipids was not essential for phagocytosis mediated by more than one receptor (yeast-igg, yeast-c3b/bi, yeast-con a). this work deals with the role of this transmembrane signaling in phagocytosis of erythrocyte (e) igg, which is mediated only by receptors for igg (fc gamma rs). the ingestion of e-igg was associated with an increase in [ca2+]i and production of in ... | 1993 | 8482923 |
| complementation of the dna repair defect in xeroderma pigmentosum group g cells by a human cdna related to yeast rad2. | defects in human dna repair proteins can give rise to the autosomal recessive disorders xeroderma pigmentosum (xp) and cockayne's syndrome (cs), sometimes even together. seven xp and three cs complementation groups have been identified that are thought to be due to mutations in genes from the nucleotide excision repair pathway. here we isolate frog and human complementary dnas that encode proteins resembling rad2, a protein involved in this pathway in yeast. alignment of these three polypeptides ... | 1993 | 8483504 |
| microdissection and microcloning of genomic dna markers from human chromosomal region 11q23. | a human genomic dna library was constructed by using a microdissection-microcloning procedure with polymerase chain reaction (pcr) techniques on dna from the chromosome 11q23 region. a total of 450 recombinant puc clones were isolated from the library. their insert sizes ranged from 150 to 850 bp with a mean of 320 bp. fifty puc clones were randomly selected and analyzed in detail. southern blot analyses showed that 21 (42%) clones were unique dna sequences, 20 (40%) clones were repetitive seque ... | 1993 | 8486352 |
| a novel form of cytochrome p-450 family 4 in human polymorphonuclear leukocytes. cdna cloning and expression of leukotriene b4 omega-hydroxylase. | isolation of cdna clones for human leukotriene b4 (ltb4) omega-hydroxylase clearly demonstrates that the hydroxylase is a member of the cytochrome p-450 (cyp) superfamily. cdna clones isolated from a human leukocyte cdna library with cyp4a4 cdna as a probe encode a protein of 520 amino acids with a molecular weight of 59,805. the deduced amino acid sequence contains an invariant cysteine in the conserved heme-binding domain near the c terminus, characteristic of the p-450 superfamily. the micros ... | 1993 | 8486631 |
| localization of seven new genes around the hla-a locus. | a yeast artificial chromosome (yac b30) with a 320 kb insert of genomic dna which includes the hla-a gene was used to screen a cdna library of human duodenal mucosa. seven cdna clones were isolated which correspond to seven new non-hla class i structural genes. these new genes are located within a region that may well contain the gene responsible for hemochromatosis and have therefore been named hcg i-vii (hemochromatosis candidate gene). hcg i, iii, v and vi are probably single copy genes, situ ... | 1993 | 8490624 |
| isolation of a candidate gene for menkes disease that encodes a potential heavy metal binding protein. | menkes disease is a lethal-x linked recessive disorder associated with copper metabolism disturbance. we have recently mapped two chromosome breakpoints related to this disease in a 1 megabase yeast artificial chromosome contig at xq13.3. we now report the construction of a phage contig and the isolation of candidate partial cdnas for the menkes disease gene. the candidate gene expresses an 8 kb message in all investigated tissues, and deletions were detected in 16% of 100 unrelated menkes patie ... | 1993 | 8490646 |
| the aspergillus nidulans ya gene is regulated by abaa. | the developmentally regulated aspergillus nidulans ya gene encodes a p-diphenol oxidase that is needed for synthesis of green conidial pigment. we subjected the ya 5' flanking region to mutational analysis in a. nidulans and saccharomyces cerevisiae to identify dna sequence elements involved in its transcriptional control, and identified two functionally distinct elements. element i contained potential brla binding sites and was required for full level ya transcription, but not for developmental ... | 1993 | 8491194 |
| phylogenetic relationships among wine yeast strains based on electrophoretic whole-cell protein patterns. | in the present work, a phylogenetic study based on protein electrophoretic profiles of saccharomyces strains isolated from different spanish wine regions has been carried out. qualitative differences between the protein electrophoregrams were found at inter- and intraspecific level, but not between electrophoregrams of strains isolated at the same ecosystem. the numerical analysis of these results allowed us to conclude that intraspecific relationships are determined by ecological factors, as we ... | 1993 | 8494678 |
| a human cdna corresponding to a gene overexpressed during cell proliferation encodes a product sharing homology with amoebic and bacterial proteins. | a clone, designated pag, was isolated by differential screening of cdna libraries made from the untransformed and ras-transformed human mammary epithelial cell line hbl100. this cdna corresponds to a gene constitutively expressed in most human cells which is induced to higher levels upon serum stimulation in untransformed and ras-transformed hbl100 cells. however, the abundance of the pag transcript is approximately 3-fold higher in transformed as compared to untransformed cells after 7-15 h of ... | 1993 | 8496166 |
| a mutational analysis of the polypyrimidine tract of introns. effects of sequence differences in pyrimidine tracts on splicing. | the polypyrimidine (py) tract of introns is required for efficient spliceosome assembly and splicing of pre-mrnas. a detailed mutational analysis of the py tract of an adenovirus 2 intron was carried out. utilizing a "precursor in pieces" vector system, it was possible to synthesize py tract mutant pre-mrnas that were otherwise identical. the mutant pre-mrnas that were otherwise identical. the mutant pre-mrnas were analyzed for in vitro splicing, for formation of splicing complexes, and for bind ... | 1993 | 8496178 |
| two yeast peroxisomal proteins crossreact with an antiserum against human sterol carrier protein 2 (scp-2). | an antibody raised against human sterol carrier protein 2 (scp-2) crossreacts with two yeast peroxisomal proteins. these proteins have apparent molecular weights of 35 and 58 kda. subfractionation of peroxisomes revealed that the 58 kda species is a soluble matrix protein, whereas the 35 kda protein is membrane bound. treatment of isolated peroxisomal membranes with 0.25 m kcl released the 35 kda crossreactive protein into the soluble supernatant. however, lipid transfer activity could be attrib ... | 1993 | 8499465 |
| multiplex pcr of three dinucleotide repeats in the prader-willi/angelman critical region (15q11-q13): molecular diagnosis and mechanism of uniparental disomy. | prader-willi syndrome (pws) and angelman syndrome (as) are distinct mental retardation disorders caused by a deficiency of paternal (pws) or maternal (as) contributions for chromosome 15 by either deletion or uniparental disomy (upd). to further study the molecular mechanisms involved in these disorders and to improve molecular diagnostic methods, we have isolated three dinucleotide repeat markers in the pws/as critical region. an alu-ca pcr method was used to isolate ca-repeat markers directly ... | 1993 | 8499903 |
| the cdk2 kinase is required for the g1-to-s transition in mammalian cells. | in the cell cycle of fission and budding yeast, the p34cdc2/cdc28 kinase is required for both the g1-to-s and g2-to-m phase transitions. in vertebrates, the homologous p34cdc2 kinase is required for g2-to-m phase transitions but appears to be dispensable for dna synthesis. we have investigated the function of a related kinase, p33cdk2, using serum-stimulated quiescent human fibroblasts. while the p33cdk2 protein was expressed at constant levels throughout the cell cycle, p33cdk2 kinase activity ... | 1993 | 8502482 |
| indication of possible post-translational formation of disulphide bonds in the beta-sheet domain of human lysozyme. | lysozyme has two distinct folding domains, and in most molecules the alpha-helical domain folds more quickly than the beta-sheet domain in vitro [radford, dobson and evans (1992) nature (london) 358, 302-307]. in order to investigate the relationship between the formation of disulphide bonds and protein folding in vivo, we carried out cysteine scanning mutagenesis to shift positions of the disulphide bonds in both the alpha-helical and beta-sheet domains of human lysozyme. of the constructed mut ... | 1993 | 8503881 |
| rat liver flavin-containing monooxygenase (fmo): cdna cloning and expression in yeast. | a rat liver cdna clone, rfmo1, coding for a flavin-containing monooxygenase (fmo) was isolated. this cdna clone encoded a protein of 532 amino acids. the deduced amino acid sequence was 84, 82 and 82% identical to those of the pig, human (form 1) and rabbit (form 1) liver fmos, while it was only 52, 50, 54, 56 and 54% identical to the human (form ii), human (form 2) and rabbit liver fmos (form 2) and rabbit and guinea pig lung fmos. rna blot analysis showed that rat liver fmo was also expressed ... | 1993 | 8504165 |
| an integrated approach for identifying and mapping human genes. | we have developed a method for generating expressed-sequence maps of human chromosomes. the method involves several steps that begin with libraries of highly representative short cdnas prepared by using random oligomers as primers. the cdna inserts are amplified by pcr with flanking vector primers. chromosomal region-specific cdna packets are prepared by hybridization of the cdna inserts to dna derived from yeast artificial chromosomes (yacs) assigned to defined regions of human chromosomes. the ... | 1993 | 8506274 |
| a putative homologue of the human autoantigen ku from saccharomyces cerevisiae. | we have identified and purified a new dna binding protein, designated hdf (high affinity dna-binding factor) from saccharomyces cerevisiae. hdf binds in a sequence-independent manner to the ends of double-stranded dna. the protein appears as a stable heterodimer of two polypeptides with molecular masses of 70 and 85 kda. we have cloned and sequenced the 70-kda subunit of the hdf protein. the amino acid sequence shows a weak but significant homology with the p70 subunit of the human ku autoantige ... | 1993 | 8509423 |
| new vector for transfer of yeast artificial chromosomes to mammalian cells. | a modification vector has been constructed to facilitate the transfer of yeast artificial chromosomes (yacs) to mammalian cells in culture by targeting a dominant selectable marker (g418 resistance) to the right arm of pyac4 clones. the ade2 gene is used for yeast selection with consequent disruption of the ura3 gene, allowing direct modification of yacs within the common host strain ab1380, and providing a simple test for correct targeting. this vector has been tested by modification of a 550-k ... | 1993 | 8511673 |
| characterization of human tissue factor pathway inhibitor variants expressed in saccharomyces cerevisiae. | human tissue factor pathway inhibitor (tfpi) and three derivatives with deletions of: 1) the complete cooh-terminal third of the polypeptide including the third kunitz domain, 2) the third kunitz domain alone, or 3) the penultimate basic cooh-terminal region alone were expressed in yeast as secreted products. high expression yield was obtained only with the derivative that lacked both the third kunitz domain and the penultimate cooh tail (tfpi1-161). the purified short form was heterogeneously g ... | 1993 | 8514773 |
| simultaneous transfer of four functional genes from the hla class ii region into mammalian cells by fusion with yeast spheroplasts carrying an artificial chromosome. | gene transfer using yeast artificial chromosome (yac) clones provides an opportunity to study the expression of several linked genes within an environment more closely approximating their normal chromosomal context. a yac clone spanning 330 kb of the hla class ii region from centromeric of tap 1 to telomeric of hla-dqa1 was retrofitted by homologous recombination with a neomycin plasmid targeted either to an alu repeat sequence within the yac genomic insert or to the ura-3 gene within the right ... | 1993 | 8515065 |
| cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases. | loss of a whole chromosome 5 or a deletion of its long arm (5q) is a recurring abnormality in malignant myeloid neoplasms. to determine the location of genes on 5q that may be involved in leukemogenesis, we examined the deleted chromosome 5 homologs in a series of 135 patients with malignant myeloid diseases. by comparing the breakpoints, we identified a small segment of 5q, consisting of band 5q31, that was deleted in each patient. this segment has been termed the critical region. distal 5q con ... | 1993 | 8516290 |
| five skeletal myosin heavy chain genes are organized as a multigene complex in the human genome. | myosin heavy chain (myhc) isoforms are encoded by a multigene family in vertebrates. we used genomic dna mapping by pulse field gel electrophoresis to demonstrate that, in humans, the embryonic, fetal, fast iib and iix myhc genes and a gene coding for a non-identified striated muscle myhc fast-type isoform (ni), are contained within a 320 kb sali genomic fragment. the locus is flanked by two cpg islands, separated by 580 kb. in order to further characterize the myhc genes, a human genomic librar ... | 1993 | 8518795 |
| molecular cloning and nucleotide sequence of the 1,2-alpha-d-mannosidase gene, msds, from aspergillus saitoi and expression of the gene in yeast cells. | a full-length cdna encoding 1,2-alpha-d-mannosidase (ec 3.2.1.113) from aspergillus saitoi was cloned. analysis of the 1718 bp nucleotide sequence of the cdna revealed a single open reading frame with 1539 nucleotides of 1,2-alpha-d-mannosidase gene, msds. the predicted amino-acid sequence of 1,2-alpha-d-mannosidase consists of 513 residues with a molecular mass of 55,767 and is 70%, 26% and 35% identity with those of penicillium citrinum 1,2-alpha-d-mannosidase, yeast alpha-mannosidase, and mou ... | 1995 | 8519794 |
| the yeast egd2 gene encodes a homologue of the alpha nac subunit of the human nascent-polypeptide-associated complex. | two saccharomyces cerevisiae proteins of 21 and 27 kda co-purify with a novel enhancer of gal4p dna binding activity (egdp) [parthun et al., mol. cell. biol. 12 (1992) 5683-5689]. mutations in the egd1 gene encoding the 21-kda protein (egd1p) have been shown to affect the kinetics and extent of the gal4p-mediated, galactose-induced activation of the gal genes. egd1p is homologous to human btf3b, recently identified as the beta subunit of the heterodimeric nascent-polypeptide-associated complex ( ... | 1995 | 8522175 |
| the ad1 transactivation domain of e2a contains a highly conserved helix which is required for its activity in both saccharomyces cerevisiae and mammalian cells. | a conserved region, designated the ad1 domain, is present in a class of helix-loop-helix (hlh) proteins, e proteins, that includes e12, e47, heb, e2-2, and a xenopus laevis hlh protein closely related to e12. we demonstrate that the ad1 domain in e2a and the conserved region of e2-2 activate transcription in both yeast and mammalian cells. the ad1 domain contains a highly conserved putative helix that is crucial for its transactivation properties. circular dichroism spectroscopy data show that a ... | 1996 | 8524288 |
| p95vav associates with the nuclear protein ku-70. | the proto-oncogene vav is expressed solely in hematopoietic cells and plays an important role in cell signaling, although little is known about the proteins involved in these pathways. to gain further information, the src homology 2 (sh2) and 3 (sh3) domains of vav were used to screen a lymphoid cell cdna library by the yeast two-hybrid system. among the positive clones, we detected a nuclear protein, ku-70, which is the dna-binding element of the dna-dependent protein kinase. in jurkat and ut7 ... | 1996 | 8524317 |
| identification of the breast cancer susceptibility gene brca2. | in western europe and the united states approximately 1 in 12 women develop breast cancer. a small proportion of breast cancer cases, in particular those arising at a young age, are attributable to a highly penetrant, autosomal dominant predisposition to the disease. the breast cancer susceptibility gene, brca2, was recently localized to chromosome 13q12-q13. here we report the identification of a gene in which we have detected six different germline mutations in breast cancer families that are ... | 1995 | 8524414 |
| cellular copper transport. | cellular copper transport processes are required by all organisms for correct utilization in cell biochemical processes and avoidance of the toxicity of copper excess. copper import into bacterial, yeast, and mammalian cells requires the coordinate function of proteins with both metal-binding and catalytic domains in mediated transport steps. following entry, detoxification mechanisms found across species include the binding of copper to specific proteins (e.g. metallothioneins) and the transfer ... | 1995 | 8527222 |
| the cic library: a large insert yac library for genome mapping in arabidopsis thaliana. | a new arabidopsis thaliana (ecotype columbia) genomic library has been constructed in yeast artificial chromosomes: the cic library (for ceph, inra and cnrs). optimization of plant culture conditions and protoplast preparation allowed the recovery of large amounts of viable protoplasts. mechanical shearing of dna was minimized by isolation of dna from protoplasts embedded in agarose. cloning of large inserts was favored by including two successive size fractionation steps (after partial ecori di ... | 1995 | 8528287 |
| mitochondrial transcription initiation: promoter structures and rna polymerases. | a diversity of promoter structures. it is evident that tremendous diversity exists between the modes of mitochondrial transcription initiation in the different eukaryotic kingdoms, at least in terms of promoter structures. within vertebrates, a single promoter for each strand exists, which may be unidirectional or bidirectional. in fungi and plants, multiple promoters are found, and in each case, both the extent and the primary sequences of promoters are distinct. promoter multiplicity in fungi, ... | 1995 | 8529266 |
| morphology of a human-derived yac in yeast meiosis. | in meiosis of human males dna is packaged along pachytene chromosomes about 20 times more compactly than in meiosis of yeast. nevertheless, a human-derived yeast artificial chromosome (yac) shows the same degree of compaction of dna as endogenous chromosomes in meiotic prophase nuclei of yeast. this suggests that in yeast meiosis, human and yeast dna adopt a similar organization of chromatin along the pachytene chromosome cores. therefore meiotic chromatin organization does not seem to be an inh ... | 1995 | 8529458 |
| a novel serine/threonine-specific protein phosphotransferase activity of nm23/nucleoside-diphosphate kinase. | two human nm23 genes have been identified, designated nm23-h1 and nm23-h2, which encode the 88% identical nucleoside-diphosphate kinase (ndpk) a and ndpk b polypeptides, respectively. the nm23-h1 gene product has been shown to play a functional role in the suppression of tumor metastasis. the nm23 proteins/ndpk are highly conserved throughout evolution and are implicated in controlling cellular differentiation and development in various species, while the underlying mechanisms remain undefined. ... | 1995 | 8529641 |
| rapid construction of integrated maps using inner product mapping: yac coverage of human chromosome 11. | inner product mapping (ipm) has been proposed as a hybridization-based method for achieving low-cost, high-throughput, high-resolution radiation hybrid (rh) mapping of clones. using alu-pcr products of chromosome 11-specific clones, we serially hybridized a set of rhs against gridded filters of yacs having an average size of 350 kb. we then combined these hybridization data with preexisting rh map data to build an inner product map. this binning of 865 yacs provides the first high-resolution lar ... | 1995 | 8530043 |
| a high-resolution map of genes, microsatellite markers, and new dinucleotide repeats from ube1 to the gata locus in the region xp11.23. | several new genes and markers have recently been identified on the proximal short arm of the human x chromosome in the area of xp11.23. we had previously generated a yac contig in this region extending from ube1 to the oatl1 locus. in this report two polymorphic dinucleotide repeats, dxs6949 and dxs6950, were isolated and characterized from the oatl1 locus. a panel of yac deletion derivatives from the distal portion of the contig was used in conjunction with the rest of the yac map to position t ... | 1995 | 8530079 |
| structure and organization of the human glucose phosphate isomerase gene (gpi). | two overlapping yeast artificial chromosome clones containing the human glucose 6-phosphate isomerase gene (gpi) have been isolated. pcr and direct sequencing were used to determine the exon/intron structure of the gene. the gene spans in excess of 40 kb and consists of 18 exons ranging in size from 44 to 153 bp. all splice sites conform to the gt/ag rule. | 1995 | 8530082 |
| identification of a family of closely related human ubiquitin conjugating enzymes. | two very closely related human e2 ubiquitin conjugating enzymes, ubfh5b and ubch5c, have been identified. these enzymes are products of distinct genes and are 88-89% identical in amino acid sequence to the recently described human e2, ubch5 (now designated ubch5a), ubch5a-c are homologous to a family of five ubiquitin conjugating enzymes from arabidopsis thaliana, atubc8-12. they are also closely related to saccharomyces cerevisiae scubc4 and scubc5, which are involved in the stress response, an ... | 1995 | 8530467 |
| the actin gene from cryptococcus neoformans: structure and phylogenetic analysis. | using heterologous probing of a genomic library, we have cloned and sequenced the actin gene from the pathogenic yeast cryptococcus neoformans. the actin gene is 1371 bp in length, and exists as a single copy, as is the case for all fungi studied to date. the locations of the introns in the c. neoformans actin gene are unique among all other known actin genes, and the deduced coding sequence results in a 375 amino acid chain with very high homology to other actins. a phylogenetic tree comprising ... | 1995 | 8531025 |
| novel aspects of the regulation of a cdna (arf1) from chlamydomonas with high sequence identity to animal adp-ribosylation factor 1. | adp-ribosylation factor (arf) is a highly conserved, low molecular mass (ca. 21 kda) gtp-binding protein that has been implicated in vesicle trafficking and signal transduction in yeast and mammalian cells. however, little is known of arf in plant systems. a putative arf polypeptide was identified in subcellular fractions of the green alga chlamydomonas reinhardtii, based on [32p]gtp binding and immunoblot assays. a cdna clone was isolated from chlamydomonas (arf1), which encodes a 20.7 kda prot ... | 1995 | 8534853 |
| production and purification of n-terminal half-transferrin in pichia pastoris. | human serum transferrin, the major iron transport protein in humans, is a monomeric glycoprotein that is composed of two homologous domains; the n-terminal domain is formed by amino acids 1-331 and the c-terminal domain is formed by amino acids 338-679. each domain is capable of binding one iron atom concomittantly with a carbonate anion; however, the two homologous iron binding sites are not chemically equivalent. the cdna sequence coding for the n-terminal domain has been cloned and overexpres ... | 1995 | 8535154 |
| [screening of yac-clones and creation of a contig covering the region of human chromosome 13, often deleted in b-cell chronic lymphocytic leukemia]. | 1995 | 8538607 | |
| cloning and characterization of a putative calcium-transporting atpase gene from schistosoma mansoni. | complementary dna was isolated, encoding a putative ca(2+)-transport atpase (sma1) of the human parasitic trematode schistosoma mansoni. the cdna was isolated by a nested polymerase chain reaction based strategy. the oligonucleotides used were designed on the basis of conserved amino-acid regions found in p-type atpases. the complete nucleotide sequence was determined. the primary structure and topology of the enzyme were deduced. sma1 has 1022 amino acids and a predicted molecular mass of 113 k ... | 1995 | 8538684 |
| [a first trial of the use of human recombinant interleukin (ril-2) in patients with tumorous diseases]. | clinical approbation of human recombinant yeast human interleukin-2 (ril-2) was carried out in 10 patients with iii-iv stages of tumor that have undergone 65 intravenous drop by drop infusions of the drug as a course of 5-11 injections in the dosage of 1-8 mln/un. the drug toxicity was shown in 4 mln and especially, in 8 mln/un dose administration. that's why the dose of 1-2 mln/un is recommended. this dose was not followed by any serious complications, and the number of slightly complicated cas ... | 1995 | 8540190 |
| expression of human cathepsin e in methylotrophic yeast, pichia pastoris. | the human gastric cathepsin e (ctse) was expressed in the methylotrophic yeast pichia pastoris by placing the ctse cdna under the control of the methanol-inducible alcohol oxidase promoter. the human ctse expressed in p. pastoris was efficiently secreted into the culture medium as an active enzyme directed by its native signal sequence, whereas ctse has been shown to be retained in mammalian tissue cells. the recombinant human ctse was secreted as a 90-kda molecule and then converted via an 84-k ... | 1995 | 8540335 |