Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
inactivation of heat adapted and chlorine adapted listeria monocytogenes atcc 7644 on tomatoes using sodium dodecyl sulphate, levulinic acid and sodium hypochlorite solution.the effectiveness of sodium dodecyl sulphate (sds), sodium hypochlorite solution and levulinic acid in reducing the survival of heat adapted and chlorine adapted listeria monocytogenes atcc 7644 was evaluated. the results against heat adapted l. monocytognes revealed that sodium hypochlorite solution was the least effective, achieving log reduction of 2.75, 2.94 and 3.97 log colony forming unit (cfu)/ml for 1, 3 and 5 minutes, respectively. sds was able to achieve 8 log reduction for both heat a ...201728713785
microorganisms of the intestinal microbiota of oncorhynchus mykiss produce antagonistic substances against bacteria contaminating food and causing disease in humans.the objective was to analyse the antibacterial ability of members of the intestinal microbiota of oncorhynchus mykiss specimens on several bacterial target strains that contaminate food and cause disease in humans. bacterial colonies from an intestinal portion of the 20 specimens of o. mykiss were obtained in different culture media. several of the colonies showed antibacterial action on different target strains. the bacterial species with the highest antagonistic capacity were hafnia alvei and ...201728713788
antimicrobial effect of black pepper petroleum ether extract for the morphology of listeria monocytogenes and salmonella typhimurium.this study aimed to evaluate the effects of black pepper petroleum extract (bppe) on pathogenic bacteria. the extraction from black pepper showed intense antimicrobial activity against the gram-positive listeria monocytogenes atcc 19115 and the gram-negative bacteria salmonella typhimurium atcc 14028. the minimum inhibitory concentrations of bppe against l. monocytogenes and s. typhimurium were 0.625 and 1.25 mg/ml, respectively. detection of alkaline phosphatase outside the cell revealed that b ...201728720964
bacterial attachment and biofilm formation on surfaces are reduced by small-diameter nanoscale pores: how small is small enough?prevention of biofilm formation by bacteria is of critical importance to areas that directly affect human health and life including medicine, dentistry, food processing and water treatment. this work showcases an effective and affordable solution for reducing attachment and biofilm formation by several pathogenic bacteria commonly associated with foodborne illnesses and medical infections.201528721236
chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans.the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ...201728725151
therapeutic options for treatment of human papillomavirus-associated cancers - novel immunologic vaccines: adxs11-001.survival of patients with advanced, recurrent, or metastatic human papillomavirus (hpv)-associated cancer is suboptimal despite the availability of various treatment modalities. the recently developed bacterial vector listeria monocytogenes (lm) activates innate and adaptive immune responses and is expected to offer immunologic advantages. axalimogene filolisbac (axal or adxs11-001) is a novel immunotherapeutic based on the live, irreversibly attenuated lm fused to the nonhemolytic fragment of l ...201728725449
genome sequences of two listeria monocytogenes strains from nectarines associated with listeriosis in 2014.listeria monocytogenes is an important foodborne pathogen. here, we present the annotated whole genome of listeria monocytogenes strains f14m01297-c2 and f14m01297-c4, isolated from nectarines distributed by a packing facility in california during an investigation of listeriosis associated with stone fruit in 2014.201728729255
early trigeminal nerve involvement in listeria monocytogenes rhombencephalitis: case series and systematic review.listeria monocytogenes is associated with rhombencephalitis. however, the exact mechanisms of brainstem invasion remains poorly understood. here, we demonstrate clinical and radiological data suggesting that listeria may invade the brainstem via the trigeminal nerve. three females (41, 64 and 70 years) with culture proven l. monocytogenes bacteremia and rhombencephalitis were investigated in the period of 2014-16. t2-weighted and contrast-enhanced t1-weighted mri revealed a cerebellopontine absc ...201728730571
an improve protocol for pcr using lm1 and lm2 primers for listeria monocytogenes detection in food matrices.several studies have observed that a conventional pcr protocol using primers lm1 and lm2 for the identification of gene hlya listeria monocytogenes generates non-specific pcr amplifications and false positives. for this reason in this study, we provide a modified pcr protocol that improves the specificity of the lm1 and lm2 primers.201728735310
listeria monocytogenes in raw milk, milking equipment and dairy workers: molecular characterization and antimicrobial resistance patterns.the aim of this study was to evaluate the genetic relatedness and patterns of antimicrobial resistance amongst l. monocytogenes isolated from raw milk, milking equipment, and hand swabs from workers in dairy farms.201728739228
nuclear carbonic anhydrase 6b associates with prmt5 to epigenetically promote il-12 expression in innate response.interleukin-12 (il-12) is critical for induction of protective immunity against intracellular bacterial infection. however, the mechanisms for efficient induction of il-12 in innate response remain poorly understood. here we report that the b type of carbonic anhydrase 6 (car6-b, which encoded ca-vi b) is essential for host defense against listeria monocytogenes (lm) infection by epigenetically promoting il-12 expression independent of its carbonic anhydrase activity. deficiency of car6-b attenu ...201728739930
antibacterial activity of timsen(®) (n-alkyl dimethyl benzyl ammonium chloride-40%) in scalding and precooling water in poultry slaughterhouses.the objective of this study was to evaluate the efficacy of a product based on n-alkyl dimethyl benzyl ammonium chloride-40%, marketed as timsen(®), during scalding and precooling of poultry carcasses in slaughterhouses. to this end, three poultry slaughterhouses (a, b and c) were evaluated. the product was added (200 ppm) to the scalding (58 °c) and precooling water (4 °c), and microbiological analyses were performed of the water and the poultry carcasses before and after timsen(®) addition. th ...201728740319
probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca.the probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca, were evaluated. this strain was selected for study from the isolated lactobacillus strains because it has specific anti-microbial activity against campylobacter jejuni atcc 33560, camp. jejuni nctc 11168, listeria monocytogenes atcc 7644, and bacillus subtilis atcc 8633. lact. curvatus dn317 showed an auto-aggregation percentage of 72% and presented the highest co-aggregation with lact. monocytoge ...201728741151
fasting metabolism modulates the interleukin-12/interleukin-10 cytokine axis.a crucial role of cell metabolism in immune cell differentiation and function has been recently established. growing evidence indicates that metabolic processes impact both, innate and adaptive immunity. since a down-stream integrator of metabolic alterations, mammalian target of rapamycin (mtor), is responsible for controlling the balance between pro-inflammatory interleukin (il)-12 and anti-inflammatory il-10, we investigated the effect of upstream interference using metabolic modulators on th ...201728742108
occurrence and antibiotic susceptibility of listeria species and staphylococcus aureus in cattle slaughterhouses of kerala, south india.a total of 765 samples were collected from beef carcasses, knives, cutting table surfaces, beef, hands, air, and water from four cattle slaughterhouses of kerala, south india, to determine the occurrence and antibiotic susceptibility of listeria species and staphylococcus aureus. listeria spp. were isolated from beef carcasses (2.0%), knives (3.7%), cutting table surfaces (1.9%), beef (0.7%), and water (1.3%). the identified species were listeria monocytogenes (0.1%), listeria innocua (0.9%), an ...201728742382
anti-adhesion and anti-biofilm potential of organosilane nanoparticles against foodborne pathogens.nowadays, modification of surfaces by nanoparticulate coatings is a simple process that may have applications in reducing the prevalence of bacterial cells both on medical devices and food processing surfaces. to this direction, biofilm biological cycle of salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7, staphylococcus aureus, and yersinia enterocolitica on stainless steel and glass surfaces, with or without nanocoating was monitored. to achieve this, four different comm ...201728744277
biogenic fabrication of cunps, cu bioconjugates and in vitro assessment of antimicrobial and antioxidant activity.in the present study, the authors synthesised copper nanoparticles (cunps) by using extract of zingiber officinale (ginger) and later the nps were bioconjugated with nisin, which shows antimicrobial activity against food spoilage microorganisms. cunps and its bioconjugate were characterised by ultraviolet-vis spectroscopy, np tracking analysis, zetasizer, transmission electron microscopy analysis, x-ray diffraction and fourier transform infra-red (ftir) spectroscopy. zeta potential of cunps and ...201728745291
a role for host cell exocytosis in inlb-mediated internalisation of listeria monocytogenes.the bacterial surface protein inlb mediates internalisation of listeria monocytogenes into human cells through interaction with the host receptor tyrosine kinase, met. inlb-mediated entry requires localised polymerisation of the host actin cytoskeleton. apart from actin polymerisation, roles for other host processes in listeria entry are unknown. here, we demonstrate that exocytosis in the human cell promotes inlb-dependent internalisation. using a probe consisting of vamp3 with an exofacial gre ...201728745416
fate of listeria innocua during production and ripening of smeared hard cheese made from raw milk.the fate of 2 different listeria innocua strains was analyzed during the production and ripening of smeared raw milk greyerzer cheese (gruyère). these strains were used as surrogates for the pathogenic listeria monocytogenes, as they are physiologically very similar. bacterial cells were added to the cheese milk at levels of 10(5) cfu/ml. during the first 24 h of cheese making, the number of the test strains decreased to a level of below 10(2) cfu/g. obviously, the cooking temperature of 56°c an ...201728755934
tpl2 promotes innate cell recruitment and effector t cell differentiation to limit citrobacter rodentium burden and dissemination.tumor progression locus 2 (tpl2) is a serine-threonine kinase that regulates th1 differentiation, secretion of the inflammatory cytokine ifnγ, and host defense against the intracellular pathogens toxoplasma gondii, listeria monocytogenes, and mycobacterium tuberculosis however, there is relatively little known about the contribution of tpl2 to th17 differentiation and immune cell function during infection with an extracellular pathogen. the goal of this study was to determine whether tpl2 influe ...201728760932
characterisation of inla truncation in listeria monocytogenes isolates from farm animals and human cases in the province of quebec.the introduction of listeria monocytogenes into the food production chain is a concern, with numerous grouped cases of listeriosis associated with milk-derived or pork-derived products have been documented. management of this zoonotic pathogen considers all strains as an equal risk. recently, a new perspective for characterisation of strain virulence was introduced with the discovery of the unaltered sequence of inla as a determinant of strain virulence; this has also been reported as an infrequ ...201728761668
the first report on listeria monocytogenes producing siderophores and responds positively to n-acyl homoserine lactone (ahl) molecules by enhanced biofilm formation.listeria monocytogenes are gram-positive well-known emerging food-borne pathogens causing listeriosis in humans. in the present study, we have isolated biofilm-forming listeria sp. from utensils used by a local milk collection dairy society at usgao goa, which collects milk for goa dairy. through biochemical tests and 16s rrna sequence analysis, the bacterium was confirmed to be l. monocytogenes and designated as strain bn3, having genbank accession number mf095110. we report for the first time ...201728762063
synthesis and in vitro evaluation of peracetyl and deacetyl glycosides of eugenol, isoeugenol and dihydroeugenol acting against food-contaminating bacteria.essential oils, as well as their separate components, have shown promise as alternatives to synthetic preservatives. therefore, it would be interesting to optimize the effect of these compounds and to evaluate their applicability as additives in food. to this end, six peracetyl and deacetyl glycosides were synthesized from eugenol, isoeugenol and dihydroeugenol. all of the glycosides were characterized by ir and nmr. the synthesized compounds and their aglycones were evaluated to determine their ...201728763946
phenolic acids and flavonoids of peanut by-products: antioxidant capacity and antimicrobial effects.peanut skin (ps) and meal from dry-blanched peanuts (mdbp) were evaluated as sources of phenolic compounds. ps rendered the highest total phenolic content, antioxidant capacity towards abts radical cation, dpph and hydroxyl radicals as well as reducing power. phenolic acids were present in ps and mdbp whereas proanthocyanidins and monomeric flavonoids were found only in ps as identified by hplc-dad-esi-ms(n). procyanidin-rich extracts prevented oxidation in non-irradiated and gamma-irradiated fi ...201728764032
listeria monocytogenes in food-processing facilities, food contamination, and human listeriosis: the brazilian scenario.listeria monocytogenes is a foodborne pathogen that contaminates food-processing environments and persists within biofilms on equipment, utensils, floors, and drains, ultimately reaching final products by cross-contamination. this pathogen grows even under high salt conditions or refrigeration temperatures, remaining viable in various food products until the end of their shelf life. while the estimated incidence of listeriosis is lower than other enteric illnesses, infections caused by l. monocy ...201728767285
multi-level strategy for identifying proteasome-catalyzed spliced epitopes targeted by cd8(+) t cells during bacterial infection.proteasome-catalyzed peptide splicing (pcps) generates peptides that are presented by mhc class i molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. applying this strategy to a murine listeria monocytogenes infection model, we identified two spliced epitopes within the secreted bacterial phospholipase plcb ...201728768206
growth inhibitory effects of adhatoda vasica and its potential at reducing listeria monocytogenes in chicken meat.the inhibitory effects of adhatoda vasica ethanolic leaf extract (avele) against listeria monocytogenes were examined to assess its potential to preserve minimally processed meat products safely. the total phenolic, flavonoid, and alkaloid levels in avele were 10.09 ± 4.52 mg of gallic acid equivalents (gae)/g, 22.43 ± 1.62 mg of quercetin equivalents/g, and 19.43 ± 3.90 mg/g, respectively. avele (1, 5, 10, or 20%) had considerable antibacterial effects against l. monocytogenes ncim 24563 in ter ...201728769879
comparative analysis of the composition and active property evaluation of certain essential oils to assess their potential applications in active food packaging.the antifungal, antibacterial, and antioxidant activity of four commercial essential oils (eos) (thyme, clove, rosemary, and tea tree) from romanian production were studied in order to assess them as bioactive compounds for active food packaging applications. the chemical composition of the oils was determined with the folin-ciocâlteu method and gas chromatography coupled with mass spectrometry and flame ionization detectors, and it was found that they respect the afnor/iso standard limits. the ...201728772407
citrus lemon essential oil: chemical composition, antioxidant and antimicrobial activities with its preservative effect against listeria monocytogenes inoculated in minced beef meat.lemon (citrus limon) is a flowing plant belonging to the rutaceae family. citrus plants constitute one of the main valuable sources of essential oil used in foods and medicinal purposes.201728774297
uv-curable contact active benzophenone terminated quaternary ammonium antimicrobials for applications in polymer plastics and related devices.a series of uv active benzophenone ([c6h5coc6h4-o-(ch2)n-n+me2r][x-], 4: r = c12h25, n = 3, x- = br- , 5a-c: r = c18h37, n = 3, x- = cl-, br-, i-; 6a-c: r = c18h37, n = 4, x- = cl-, br-, i- ; 7a-c: r = c18h37, n = 6, x- = cl-, br-, i-) terminated c12 and c18 quaternary ammonium salts (qacs) were prepared by thermal or microwave driven menshutkin protocols of the appropriate benzophenone alkyl halide (1a-c, 2a-c, 3a-c) with the corresponding dodecyl- or octadecyl n,n-dimethylamine. all new compou ...201728777541
progranulin deficiency causes impairment of autophagy and tdp-43 accumulation.loss-of-function mutations in grn cause frontotemporal dementia (ftd) with transactive response dna-binding protein of 43 kd (tdp-43)-positive inclusions and neuronal ceroid lipofuscinosis (ncl). there are no disease-modifying therapies for either ftd or ncl, in part because of a poor understanding of how mutations in genes such as grn contribute to disease pathogenesis and neurodegeneration. by studying mice lacking progranulin (pgrn), the protein encoded by grn, we discovered multiple lines of ...201728778989
recent advances in understanding listeria monocytogenes infection: the importance of subcellular and physiological context.the bacterial pathogen listeria monocytogenes ( lm) is the causative agent of listeriosis, a rare but fatal foodborne disease. during infection, lm can traverse several host barriers and enter the cytosol of a variety of cell types. thus, consideration of the extracellular and intracellular niches of lm is critical for understanding the infection process. here, we review advances in our understanding of lm infection and highlight how the interactions between the host and the pathogen are context ...201728781746
critical roles of mtorc1 signaling and metabolic reprogramming for m-csf-mediated myelopoiesis.myelopoiesis is necessary for the generation of mature myeloid cells during homeostatic turnover and immunological insults; however, the metabolic requirements for this process remain poorly defined. here, we demonstrate that myelopoiesis, including monocyte and macrophage differentiation, requires mechanistic target of rapamycin complex 1 (mtorc1) signaling and anabolic metabolism. loss of mtorc1 impaired myelopoiesis under steady state and dampened innate immune responses against listeria mono ...201728784627
a rare case of meningoencephalitis by listeria monocytogenes in systemic lupus erythematosus: case report and review.patients with systemic lupus erythematosus (sle) have a high risk of infection. central nervous system infection and neuropsychiatric sle are both major causes of death. it is vital to distinguish between these two conditions to improve prognosis due to the treatment paradigms required for each condition. here, we report one case of meningoencephalitis by listeria monocytogenes (lm) in a patient with sle who presented with fever and developed headache and altered in consciousness in the hospital ...201728785856
sulfadiazine-chitosan conjugates and their polyelectrolyte complexes with hyaluronate destined to the management of burn wounds.in the present study polyelectrolyte complexes (pecs) based on new sulfadiazine-chitosan conjugates with sodium hyaluronate have been developed with potential use in treatment of burn wounds. the pecs were chemically characterized using fourier transform-infrared spectroscopy, scanning electon microscopy and near infrared chemical imaging technique. the swelling behavior and in vitro sulfadiazine release were also investigated. the antimicrobial activity was evaluated towards three bacterial str ...201528787940
fate of listeria monocytogenes on fresh apples under different storage temperatures.fresh apples are typically stored for up to 1 year commercially; different apple varieties require different storage temperatures to maintain their quality characteristics. there is sparse information available about listeria monocytogenes survival on fresh apples under various storage temperatures. the objective of this study was to comprehensively evaluate the effect of storage temperature on apple fruit decay and l. monocytogenes survival. unwaxed apple fruits of selected varieties (fuji and ...201728790993
listeria in adults - truly rare or rarely diagnosed in india?listeria monocytogenes is a facultative anaerobic intracellular gram positive rod causing infection in pregnant women, extremes of age and immune-compromised hosts. in clinical specimens, the organisms may be gram-variable: laboratory misidentification of l. monocytogenes isolates as diphtheroids, streptococci, or enterococci is not uncommon and the isolation of a diphtheroid from blood or csf should always alert the clinician to the possibility that the organism may be l. monocytogenes. the dis ...201728792179
synergy of a combination of nisin and citric acid against staphylococcus aureus and listeria monocytogenes.food-borne diseases caused by pathogens, such as staphylococcus aureus and listeria monocytogenes, have long attracted attention globally from researchers, food industries, and food safety authorities. nisin (ns) is the only bacteriocin used worldwide as a generally recognised as safe (gras) food preservative, while citric acid (ca) has an unrestricted use in foods since it has gras status. in this study, synergistic interactions of ns combined with ca against s. aureus and l. monocytogenes were ...201728795907
holographic deep learning for rapid optical screening of anthrax spores.establishing early warning systems for anthrax attacks is crucial in biodefense. despite numerous studies for decades, the limited sensitivity of conventional biochemical methods essentially requires preprocessing steps and thus has limitations to be used in realistic settings of biological warfare. we present an optical method for rapid and label-free screening of bacillus anthracis spores through the synergistic application of holographic microscopy and deep learning. a deep convolutional neur ...201728798957
mathematical model of interaction between bacteriocin-producing lactic acid bacteria and listeria. part 2: bifurcations and applications.the big challenge for the food industry is the attending to demands for minimally processed foods, avoiding intense heat treatments and reducing the addition of chemical preservatives, but at the same time ensuring microbiological safety of these products. lactic acid bacteria are traditionally used in the production of fermented foods. they are responsible for the production of antimicrobial compounds, such as organic acids and bacteriocins, which are protein compounds with bactericidal effect ...201728799082
listeria monocytogenes incidence changes and diversity in some brazilian dairy industries and retail products.listeria monocytogenes can cause listeriosis, a severe foodborne disease. in brazil, despite very few reported cases of listeriosis, the pathogen has been repeatedly isolated from dairies. this has led the government to implement specific legislation to reduce the hazard. here, we determined the incidence of l. monocytogenes in five dairies and retail products in the southeast and midwest regions of brazil over eight months. of 437 samples, three samples (0.7%) from retail and only one sample (0 ...201728800821
combination of endolysins and high pressure to inactivate listeria monocytogenes.outbreaks of listeriosis are often related to the consumption of low-processed ready-to-eat food products (e.g. soft cheeses or smoked fish) contaminated with listeria monocytogenes. traditional preservation techniques, such as heat treatment, cannot eliminate listeria from these products without strongly affecting the quality of the foods. we therefore investigated the use of endolysin (plyp40, ply511, or plyp825) in combination with high hydrostatic pressure processing to kill l. monocytogenes ...201728800829
modeling carbon dioxide effect in a controlled atmosphere and its interactions with temperature and ph on the growth of l. monocytogenes and p. fluorescens.the effect of carbon dioxide, temperature, and ph on growth of listeria monocytogenes and pseudomonas fluorescens was studied, following a protocol to monitor microbial growth under a constant gas composition. in this way, the co2 dissolution didn't modify the partial pressures in the gas phase. growth curves were acquired at different temperatures (8, 12, 22 and 37 °c), ph (5.5 and 7) and co2 concentration in the gas phase (0, 20, 40, 60, 80, 100% of the atmospheric pressure, and over 1 bar). t ...201728800830
characterization of enterococcus durans 152 bacteriocins and their inhibition of listeria monocytogenes in ham.listeria monocytogenes is a nonfastidious, widely occurring foodborne pathogen that is a major challenge to the food industry. enterococcus durans 152, a confirmed l. monocytogenes-control microorganism, was isolated from floor drain samples from a food processing facility. in this study, the two bacteriocins produced by e. durans 152 were characterized and identified as dur 152a (an enterocin l50a derivative with two amino acid substitutions of i→m) and enterocin l50b. the bacteriocins were the ...201728800831
replication-transcription conflicts generate r-loops that orchestrate bacterial stress survival and pathogenesis.replication-transcription collisions shape genomes, influence evolution, and promote genetic diseases. although unclear why, head-on transcription (lagging strand genes) is especially disruptive to replication and promotes genomic instability. here, we find that head-on collisions promote r-loop formation in bacillus subtilis. we show that pervasive r-loop formation at head-on collision regions completely blocks replication, elevates mutagenesis, and inhibits gene expression. accordingly, the ac ...201728802046
two-step large-volume magnetic separation combined with pcr assay for sensitive detection of listeria monocytogenes in pasteurized milk.immunomagnetic separation (ims) is an effective tool for the preconcentration and purification of food-borne pathogens from complex food samples because of its high capture efficiency (ce). in conventional ims, antibodies are usually conjugated on the surface of magnetic beads (mb); the random orientation and conformation changes of antibodies on the mb surface can decrease their bioactivity. moreover, the brownian motion of immobilized antibodies is weakened, thereby rendering their binding eff ...201728803008
short communication: detection of stx2 and elt genes in bovine milk by using a multiplex pcr system.the aim of this study was to detect 2 important toxin genes from diarrheagenic escherichia coli (dec) in bovine milk using a new multiplex pcr. to standardize the multiplex pcr, the stx2 and elt genes were investigated for the detection of shiga toxin-producing escherichia coli (stec) and enterotoxigenic e. coli (etec), respectively. the dna template was prepared with a thermal procedure (boiling) and a commercial kit. samples consisted of uht and pasteurized milk, both skimmed, and stec and ete ...201728803013
sequential transition of the injury phenotype, temperature-dependent survival and transcriptional response in listeria monocytogenes following lethal h2o2 exposure.the food-borne pathogen listeria monocytogenes is present persistently in food processing environments, where this bacterium is exposed to various stress factors, including oxidative stress. this study aimed to elucidate the temperature-dependent response of l. monocytogenes to h2o2 exposure and the phenotypic changes in colony formation by h2o2-treated bacteria. survival curves indicated an increase in the resistance to h2o2 in l. monocytogenes as the temperature decreased during the stress exp ...201728803156
efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces.air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ...201728803189
recombinant lcmv vectors induce protective immunity following homologous and heterologous vaccinations.successful vaccination against cancer and infectious diseases relies on the induction of adaptive immune responses that induce high-titer antibodies or potent cytoxic t cell responses. in contrast to humoral vaccines, the amplification of cellular immune responses is often hampered by anti-vector immunity that either pre-exists or develops after repeated homologous vaccination. replication-defective lymphocytic choriomeningitis virus (lcmv) vectors represent a novel generation of vaccination vec ...201728803862
investigating the susceptibility of mice to a bacterial challenge after intravenous exposure to durable nanoparticles.the goal of this study was to determine whether bacterial clearance in a rodent model would be impaired upon exposure to gold, silver or silica nanoparticles (nps).201728805153
selection of surrogate bacteria for use in food safety challenge studies: a review.nonpathogenic surrogate bacteria are prevalently used in a variety of food challenge studies in place of foodborne pathogens such as listeria monocytogenes, salmonella, escherichia coli o157:h7, and clostridium botulinum because of safety and sanitary concerns. surrogate bacteria should have growth characteristics and/or inactivation kinetics similar to those of target pathogens under given conditions in challenge studies. it is of great importance to carefully select and validate potential surr ...201728805457
thermal inactivation of listeria monocytogenes and salmonella during water and steam blanching of vegetables.thermal inactivation of listeria monocytogenes and salmonella was evaluated on peas, spinach, broccoli, potatoes, and carrots that were treated with hot water and steam. one gram-positive bacterium, l. monocytogenes, and one gram-negative bacterium, salmonella, were selected as pertinent human pathogens for evaluation. samples were inoculated with a composite of five strains each of l. monocytogenes and salmonella to achieve approximately 10(8) to 10(9) cfu/g. inoculated samples were treated wit ...201728805458
agonist anti-gitr antibody significantly enhances the therapeutic efficacy of listeria monocytogenes-based immunotherapy.we previously demonstrated that in addition to generating an antigen-specific immune response, listeria monocytogenes (lm)-based immunotherapy significantly reduces the ratio of regulatory t cells (tregs)/cd4(+) and myeloid-derived suppressor cells (mdscs) in the tumor microenvironment. since lm-based immunotherapy is able to inhibit the immune suppressive environment, we hypothesized that combining this treatment with agonist antibody to a co-stimulatory receptor that would further boost the ef ...201728807056
structural and functional studies of pyruvate carboxylase regulation by cyclic di-amp in lactic acid bacteria.cyclic di-3',5'-adenosine monophosphate (c-di-amp) is a broadly conserved bacterial second messenger that has been implicated in a wide range of cellular processes. our earlier studies showed that c-di-amp regulates central metabolism in listeria monocytogenes by inhibiting its pyruvate carboxylase (lmpc), a biotin-dependent enzyme with biotin carboxylase (bc) and carboxyltransferase (ct) activities. we report here structural, biochemical, and functional studies on the inhibition of lactococcus ...201728808024
listeria monocytogenes has both a bd-type and an aa3 -type terminal oxidase which allow growth in different oxygen levels and both are important in infection.listeria monocytogenes is a food borne pathogen responsible for a number of life-threatening infections of humans. during an infection it invades epithelial cells before spreading from the intestine to the cells of the liver and spleen. this requires an ability to adapt to varying oxygen levels. here we demonstrate that l. monocytogenes has two terminal oxidases, a cytochrome bd-type (cydab) and a cytochrome aa3 -type menaquinol oxidase (qoxab) and that both are used for respiration under differ ...201728808161
listeria monocytogenes and shigella flexneri activate the nlrp1b inflammasome.activation of the innate immune receptor nlrp1b leads to the formation of an inflammasome, which induces autoproteolytic processing of pro-caspase-1, and ultimately to the release of inflammatory cytokines and to the execution of pyroptosis. one of the signals to which nlrp1b responds is metabolic stress that occurs in cells deprived of glucose or treated with metabolic inhibitors. nlrp1b might, therefore, sense microbial infection as intracellular pathogens such as listeria monocytogenes and sh ...201728808162
aggregation-induced-emission materials with different electric charges as an artificial tongue: design, construction, and assembly with various pathogenic bacteria for effective bacterial imaging and discrimination.imaging-based total bacterial count and type identification of bacteria play crucial roles in clinical diagnostics, public health, biological and medical science, and environmental protection. herein, we designed and synthesized a series of tetraphenylethenes (tpes) functionalized with one or two aldehyde, carboxylic acid, and quaternary ammonium groups, which were successfully used as fluorescent materials for rapid and efficient staining of eight kinds of representative bacterial species, incl ...201728809473
the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells.six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ...201728811611
agonist anti-gitr antibody significantly enhances the therapeutic efficacy of listeria monocytogenes-based immunotherapy.we previously demonstrated that in addition to generating an antigen-specific immune response, listeria monocytogenes (lm)-based immunotherapy significantly reduces the ratio of regulatory t cells (tregs)/cd4(+) and myeloid-derived suppressor cells (mdscs) in the tumor microenvironment. since lm-based immunotherapy is able to inhibit the immune suppressive environment, we hypothesized that combining this treatment with agonist antibody to a co-stimulatory receptor that would further boost the ef ...201728815044
use of whey as a culture medium for bacillus clausii for the production of protein hydrolysates with antimicrobial and antioxidant activity.the use of whey as a natural culture medium to produce hydrolysates with antimicrobial from bacillus clausii is the objective of this research. the fermentation process was carried out at 25 ℃ for five days, where proteolytic activity, soluble peptides quantification, and antimicrobial activity using the disc diffusion method were determined every day. the fermented whey reached values ​​of proteolytic activity between 90 and 260 u ml(-1) and a production of soluble peptides between 100 and 2070 ...201728816527
antimicrobial activity and acetylcholinesterase inhibition by extracts from chromatin modulated fungi.major health challenges as the increasing number of cases of infections by antibiotic multiresistant microorganisms and cases of alzheimer's disease have led to searching new control drugs. the present study aims to verify a new way of obtaining bioactive extracts from filamentous fungi with potential antimicrobial and acetylcholinesterase inhibitory activities, using epigenetic modulation to promote the expression of genes commonly silenced. for such finality, five filamentous fungal species (t ...201728818332
estimated annual numbers of foodborne pathogen-associated illnesses, hospitalizations, and deaths, france, 2008-2013.estimates of the annual numbers of foodborne illnesses and associated hospitalizations and deaths are needed to set priorities for surveillance, prevention, and control strategies. the objective of this study was to determine such estimates for 2008-2013 in france. we considered 15 major foodborne pathogens (10 bacteria, 3 viruses, and 2 parasites) and estimated that each year, the pathogens accounted for 1.28-2.23 million illnesses, 16,500-20,800 hospitalizations, and 250 deaths. campylobacter ...201728820137
preparation, characterization and antibacterial activity of oxidized κ-carrageenan.the oxidized κ-carrageenans with different oxidation levels were prepared through the hydrogen peroxide and copper sulfate redox system. the oxidation level of oxidized κ-carrageenan was successfully controlled by adjusting the dosage of hydrogen peroxide. the results showed that the microtopography of oxidized κ-carrageenan changed from rough granules to smooth flakes, mainly resulting from the easily melting property of oxidized κ-carrageenan induced by introduced carboxyl and aldehyde groups. ...201728821027
chemical composition of mentha pulegium and rosmarinus officinalis essential oils and their antileishmanial, antibacterial and antioxidant activities.the aim of the study was the determination of the chemical composition of mentha pulegium l. and rosmarinus officinalis l. essential oils and the evaluation of their antileishmanial, antibacterial and antioxidant activities. essential oils (eos) were isolated using steam distillation and the chemical composition was determined using gc-ms analysis. the antibacterial activity was tested against ten pathogenic strains using the diffusion method, the minimum inhibitory concentration (mic) and the m ...201728821401
a screen for kinase inhibitors identifies antimicrobial imidazopyridine aminofurazans as specific inhibitors of the listeria monocytogenes pasta kinase prka.bacterial signaling systems such as protein kinases and quorum sensing have become increasingly attractive targets for the development of novel antimicrobial agents in a time of rising antibiotic resistance. the family of bacterial penicillin-binding-protein and serine/threonine kinase-associated (pasta) kinases is of particular interest due to the role of these kinases in regulating resistance to β-lactam antibiotics. as such, small-molecule kinase inhibitors that target pasta kinases may prove ...201728821610
a suicidal strain of listeria monocytogenes is effective as a dna vaccine delivery system for oral administration.in this study we determined the in vivo activity of model ovalbumin vaccines delivered by direct intramuscular delivery of plasmid dna or oral delivery using a recombinant suicidal listeria monocytogenes strain (rsδ2). in a previous report we described how rsδ2 is capable of delivering luciferase, as protein or dna, in vitro, into non-dividing intestinal epithelial cells (kuo et al., 2009). this is achieved by engineering a dual expression shuttle vector, pdulx-luc, that replicates in e. coli an ...201728822642
prospects and progress of listeria-based cancer vaccines.the development of an effective therapeutic vaccine to induce cancer-specific immunity remains problematic. recently, a species of intracellular pathogen known as listeria monocytogenes (lm) has been used to transfer dna, rna and proteins into tumour cells as well as elicit an immune response against tumour-specific antigens. areas covered: herein, the authors provide the mechanisms of different listeria monocytogenes strains, which are potential therapeutic cancer vaccine vectors, in addition t ...201728823183
molecular identification of disk abalone (haliotis discus discus) tetraspanin 33 and cd63: insights into potent players in the disk abalone host defense system.tetraspanins are a superfamily of transmembrane proteins involved in a diverse range of physiological processes including differentiation, adhesion, signal transduction, cell motility, and immune responses. in the present study, two tetraspanins, cd63 and tetraspanin 33 (tspan33) from disk abalone (abcd63 and abtspan33), were identified and characterized at the molecular level. the coding sequences for abcd63 and abtspan33 encoded polypeptides of 234 and 290 amino acids (aa) with predicted molec ...201728823981
clec5a is a critical receptor in innate immunity against listeria infection.the c-type lectin member 5a (clec5a) is a pattern recognition receptor for members of the flavivirus family and has critical functions in response to dengue virus and japanese encephalitis virus. here we show that clec5a is involved in neutrophil extracellular trap formation and the production of reactive oxygen species and proinflammatory cytokines in response to listeria monocytogenes. inoculation of clec5a (-/-) mice with l. monocytogenes causes rapid bacterial spreading, increased bacterial ...201728824166
comparison of the microbiological quality and safety between conventional and organic vegetables sold in malaysia.given the remarkable increase of public interest in organic food products, it is indeed critical to evaluate the microbiological risk associated with consumption of fresh organic produce. organic farming practices including the use of animal manures may increase the risk of microbiological contamination as manure can act as a vehicle for transmission of foodborne pathogens. this study aimed to determine and compare the microbiological status between organic and conventional fresh produce at the ...201728824567
evaluation of 3m molecular detection assay (mda) 2-listeria for the detection of listeria species in select foods and environmental surfaces: collaborative study, first action 2016.07.3m molecular detection assay (mda) 2-listeria uses loop-mediated isothermal amplification and bioluminescence detection to rapidly detect listeria species in a broad range of food types and environmental surfaces. using an unpaired study design, mda 2-listeria was compared with the u.s. department of agriculture, food safety and inspection service's microbiology laboratory guidebook chapter 8.09 "isolation and identification of listeria monocytogenes from red meat, poultry and egg products, and ...201728825539
crystal diagnostics xpress(™) lm kit for the rapid detection of listeria monocytogenes from environmental surfaces.the crystal diagnostics (cdx) xpress™ lm kit is used for rapid screening of low concentrations of listeria monocytogenes on environmental surfaces such as stainless steel, plastic, and ceramic tile. in addition to the xpress lm kit, the cdx xpress system comprises an automatic xpress reader, a biocassette™ that incorporates antibody-coupled microspheres, and liquid crystal for selective identification of the intended microbe. all 56 of the 56 tested l. monocytogenes strains evaluated were detect ...201728825544
synthesis and antimicrobial studies of new antibacterial azo-compounds active against staphylococcus aureus and listeria monocytogenes.some novel (phenyl-diazenyl)phenols (4a-m) were designed and synthesized to be evaluated for their antibacterial activity. starting from an active previously-synthesized azobenzene chosen as lead compound, we introduced some modifications and optimization of the structure, in order to improve solubility and drug conveyance. structures of all newly-synthesized compounds were confirmed by ¹h nuclear magnetic resonance (nmr), mass spectrometry, and uv-vis spectroscopy. antibacterial activity of the ...201728825625
spontaneous virulence loss in natural populations of listeria monocytogenes.listeria monocytogenes (lm) pathogenesis depends on its ability to escape from the phagosome of the host cells via the action of the pore-forming toxin listeriolysin o (llo). expression of the llo-encoding gene (hly) requires the transcriptional activator prfa, and both hly and prfa genes are essential for lm virulence. here we used the hemolytic activity of llo as a phenotypic marker to screen for spontaneous virulence-attenuating mutations in lm sixty (0.1%) non-hemolytic isolates were identif ...201728827366
protein kinase c-delta (pkcδ), a marker of inflammation and tuberculosis disease progression in humans, is important for optimal macrophage killing effector functions and survival in mice.we previously demonstrated that protein kinase c-δ (pkcδ) is critical for immunity against listeria monocytogenes, leishmania major, and candida albicans infection in mice. however, the functional relevance of pkcδ during mycobacterium tuberculosis (mtb) infection is unknown. pkcδ was significantly upregulated in whole blood of patients with active tuberculosis (tb) disease. lung proteomics further revealed that pkcδ was highly abundant in the necrotic and cavitory regions of tb granulomas in mu ...201728832027
simultaneous detection of escherichia coli o157:h7, salmonella enteritidis, and listeria monocytogenes at a very low level using simultaneous enrichment broth and multichannel spr biosensor.detection of foodborne pathogens at very low levels is still a challenge. a custom-built multichannel surface plasmon resonance (spr) biosensor and simultaneous enrichment broth (seb) were used to develop a simultaneous detection method for 3 important foodborne pathogens, escherichia coli o157:h7 (o157:h7), salmonella enteritidis, and listeria monocytogenes, at a very low level. these 3 foodborne pathogens at a very low level (14, 6, and 28 cfu/25 g (ml) for o157:h7, s. enteritidis, and l. mono ...201728833106
in vitro characterization of lactobacillus strains isolated from fruit processing by-products as potential probiotics.nine wild lactobacillus strains, namely lactobacillus plantarum 53, lactobacillus fermentum 56, l. fermentum 60, lactobacillus paracasei 106, l. fermentum 250, l. fermentum 263, l. fermentum 139, l. fermentum 141, and l. fermentum 296, isolated from fruit processing by-products were evaluated in vitro for a series of safety, physiological functionality, and technological properties that could enable their use as probiotics. considering the safety aspects, the resistance to antibiotics varied amo ...201728836171
lactolisterin bu, a novel class ii broad spectrum bacteriocin from lactococcus lactis subsp. lactis bv. diacetylactis bgbu1-4.lactococcus lactis subsp. lactis bv. diacetylactis bgbu1-4 produces a novel bacteriocin, lactolisterin bu, with strong antimicrobial activity against many species of gram-positive bacteria, including important food spoilage and food-borne pathogens such as listeria monocytogenes, staphylococcus aureus, bacillus sp. and streptococci. lactolisterin bu was extracted from the cell surface of bgbu1-4 by propan-2-ol and purified to homogeneity by c18 solid phase extraction and reversed phase hplc. the ...201728842543
the arsenic resistance listeria genomic island lgi2 exhibits sequence and integration site diversity and propensity for three listeria monocytogenes clones with enhanced virulence.in the foodborne pathogen listeria monocytogenes, arsenic resistance is primarily encountered in serotype 4b clones considered to have enhanced virulence, and is associated with an arsenic resistance gene cluster within a 35 kb chromosomal region, listeria genomic island 2 (lgi2). lgi2 was first identified in strain scott a and includes genes putatively involved in arsenic and cadmium resistance, dna integration, conjugation and pathogenicity. however, genomic localization and sequence content o ...201728842547
utilization of industrial waste for the production of bio-preservative from bacillus licheniformis me1 and its application in milk and milk-based food products.the bio-preservative efficacy of a partially purified antibacterial peptide (ppabp) produced by bacillus licheniformis me1 in an economical medium developed using agro-industry waste was evaluated by direct application in milk and milk-based food products. the addition of ppabp in milk samples stored at 4 ± 2 °c and 28 ± 2 °c resulted in the growth inhibition of pathogens listeria monocytogenes scott a, micrococcus luteus atcc 9341, and staphylococcus aureus fri 722. the shelf life of milk sampl ...201728842843
lactobionic acid enhances the synergistic effect of nisin and thymol against listeria monocytogenes scott a in tryptic soy broth and milk.listeria monocytogenes is a gram-positive opportunistic human pathogen and it remains a significant cause of foodborne illnesses. a variety of natural and synthetic compounds have been studied to inhibit the growth of l. monocytogenes in foods. antimicrobial combinations with synergistic antilisterial properties can reduce the dose of each antimicrobial, which can be further enhanced by chelating compounds. therefore, the objective of this study was to determine antilisterial properties of binar ...201728843122
antimicrobial efficacy of cinnamomum javanicum plant extract against listeria monocytogenes and its application potential with smoked salmon.extracts from medicinal plants have been reported to possess good antimicrobial properties, but a majority of them remain unexplored. this study aimed at identifying a novel plant extract with antimicrobial activity, to validate its efficacy in food model, and to elucidate its composition and antimicrobial mechanism. a total of 125 plant extracts were screened, and cinnamomum javanicum leaf and stem extract showed potential antimicrobial activity against listeria monocytogenes (mic=0.13mg/ml). t ...201728843123
impact of the reusing of food manufacturing wastewater for irrigation in a closed system on the microbiological quality of the food crops.in order to evaluate if the reuse of food industry treated wastewater is compatible for irrigation of food crops, without increased health risk, in the present study a cropping system, in which ground water and treated wastewater were used for irrigation of tomato and broccoli, during consecutive crop seasons was monitored. water, crop environment and final products were monitored for microbial indicators and pathogenic bacteria, by conventional and molecular methods. the microbial quality of th ...201728843124
impact of cooking procedures and storage practices at home on consumer exposure to listeria monocytogenes and salmonella due to the consumption of pork meat.the objective of this research was to analyze the impact of different cooking procedures (i.e., gas hob and traditional static oven) and levels of cooking (i.e., rare, medium, and well-done) on inactivation of listeria monocytogenes and salmonella in pork loin chops. moreover, the consumer's exposure to both microorganisms after simulation of meat leftover storage at home was assessed. the results showed that well-done cooking in a static oven was the only treatment able to inactivate the tested ...201728846810
application of nanocompostie chitosan and carboxymethyl cellulose films containing natural preservative compounds in minced camel's meat.in the present study, novel films based on nanomontmorillonite-chitosan (mmt-ch) and nanomontmorillonite-carboxymethyl cellulose (mmt-cmc) incorporated with different concentrations of ziziphora clinopodioides essential oil (zeo; 0.5, 1 and 2%) alone and in combination with ficus carica extract (fch; 1%) were investigated as active packaging materials for minced camel's meat to increase the shelf life (microbial, chemical and sensory properties) and inhibit the growth of listeria monocytogenes a ...201728847602
multiple cortical brain abscesses due to listeria monocytogenes in an immunocompetent patient.listeria monocytogenes is an intracellular organism which is well recognised for its ability to cause meningeal infections in neonates, immunosuppressed, debilitated and elderly individuals. (1) other less common central nervous system (cns) infections caused by listeria spp. include rhomboencephalitis, cerebritis and abscesses in the brain, brain stem and spinal cord. the neuroradiological appearance of listeria brain abscesses is similar to other types and may also mimic primary or metastatic ...201728849735
occurrence and growth of listeria monocytogenes in packaged raw milk.the increased availability of packaged raw drinking milk necessitates the investigation of the occurrence and growth of listeria monocytogenes in raw milk during distribution and storage. the occurrence of l. monocytogenes in 105 retailed raw milk bottles, 115 bulk tank milk samples, 23 in-line milk filter socks and in 50 environmental samples collected from an on-farm dairy establishment were investigated. growth of inoculated low-level l. monocytogenes contamination was also investigated in tw ...201728850852
in vitro antimicrobial activity screening of xylaria hypoxylon.fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey.201628852719
corrigendum: growth inhibitory effects of adhatoda vasica and its potential at reducing listeria monocytogenes in chicken meat.[this corrects the article on p. 1260 in vol. 8, pmid: 28769879.].201728855897
genomic characterization helps dissecting an outbreak of listeriosis in northern italy.listeria monocytogenes (lm) is a bacterium widely distributed in nature and able to contaminate food processing environments, including those of dairy products. lm is a primary public health issue, due to the very low infectious dose and the ability to produce severe outcomes, in particular in elderly, newborns, pregnant women and immunocompromised patients.201728856063
lactococcus lactis and lactobacillus salivarius bgbu1-4 differently modulate early immunological response of wistar rats co-administered with listeria monocytogenes.in the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. accordingly, the aim of this study was to test whether two lactic acid bacteria (lab) strains, lactococcus lactis bgbu1-4 and lactobacillus salivarius bgho1, could be applied as safe supplements for listeria infection. two major research objectiv ...201728856909
Displaying items 16701 - 16792 of 16792