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impact of environmental factors on the culturability and viability of listeria monocytogenes under conditions encountered in food processing plants.the ability of listeria monocytogenes to adhere to and persist on surfaces for months or even years may be responsible for its transmission from contaminated surfaces to food products. hence the necessity to find effective means to prevent the establishment of l. monocytogenes in food processing environments. the aim of this study was to assess, through a fractional experimental design, the environmental factors that could affect the survival of l. monocytogenes cells on surfaces to thereby prev ...201728073080
antibacterial effect of 405±5nm light emitting diode illumination against escherichia coli o157:h7, listeria monocytogenes, and salmonella on the surface of fresh-cut mango and its influence on fruit quality.to investigate a potential of 405±5nm light emitting diode (led) as a novel technology for food preservation, the antibacterial effect of 405±5nm led on escherichia coli o157:h7, listeria monocytogenes, and salmonella spp. on the surface of fresh-cut mango and its influence on fruit quality were evaluated at different storage temperatures. led-illumination inactivated 1.0-1.6 logcfu/cm(2) of populations at 4 and 10°c for 36-48h (total dose, 2.6-3.5kj/cm(2)) regardless of bacterial species, while ...201728073081
promyelocytic leukemia protein (pml) controls listeria monocytogenes infection.the promyelocytic leukemia protein (pml) is the main organizer of stress-responsive subnuclear structures called pml nuclear bodies. these structures recruit multiple interactors and modulate their abundance or their posttranslational modifications, notably by the sumo ubiquitin-like modifiers. the involvement of pml in antiviral responses is well established. in contrast, the role of pml in bacterial infection remains poorly characterized. here, we show that pml restricts infection by the patho ...201728074026
prevalence, virulence potential, and antibiotic susceptibility profile of listeria monocytogenes isolated from bovine raw milk samples obtained from rajasthan, india.listeriosis is a serious foodborne disease of a global concern, and can effectively be controlled by a continuous surveillance of the virulent and multidrug-resistant strains of listeria monocytogenes. this study was planned to investigate prevalence of l. monocytogenes in bovine raw milk samples. a total of 457 raw milk samples collected from 15 major cities in rajasthan, india, were analyzed for the presence of l. monocytogenes by using standard microbiological and molecular methods. five of t ...201728085487
bcap inhibits proliferation and differentiation of myeloid progenitors in the steady state and during demand situations.b-cell adaptor for phosphatidylinositol 3-kinase (bcap) is a signaling adaptor expressed in mature hematopoietic cells, including monocytes and neutrophils. here we investigated the role of bcap in the homeostasis and development of these myeloid lineages. bcap(-/-) mice had more bone marrow (bm) monocytes than wild-type (wt) mice, and in mixed wt:bcap(-/-) bm chimeras, monocytes and neutrophils skewed toward bcap(-/-) origin, showing a competitive advantage for bcap(-/-) myeloid cells. bcap was ...201728087538
acquired central hypoventilation following listeria monocytogenes rhombencephalitis. 201728087753
comprehensive proteome analysis of lysosomes reveals the diverse function of macrophages in immune responses.phagocytosis and autophagy in macrophages have been shown to be essential to both innate and adaptive immunity. lysosomes are the main catabolic subcellular organelles responsible for degradation and recycling of both extracellular and intracellular material, which are the final steps in phagocytosis and autophagy. however, the molecular mechanisms underlying lysosomal functions after infection remain obscure. in this study, we conducted a quantitative proteomics analysis of the changes in const ...201728088779
anti-pathogenic and probiotic attributes of lactobacillus salivarius and lactobacillus plantarum strains isolated from feces of algerian infants and adults.sixty-seven (67) lactic acid bacteria (lab) isolates belonging to lactobacillus genus were isolated from human feces and tested for their auto-aggregation and cell surface hydrophobicity in order to establish their adhesion capabilities, a prerequisite for probiotic selection. strains with the upmost auto-aggregation and cell surface hydrophobicity scores were identified by maldi-tof spectrometry and 16s rdna sequencing as lactobacillus plantarum (p25lb1 and p98lb1) and lactobacillus salivarius ...201728093322
functional constituents of wild and cultivated goji (l. barbarum l.) leaves: phytochemical characterization, biological profile, and computational studies.goji (lycium barbarum l.) leaves are emphasized as a functional tea or as dietary supplements. the phenolic compound profile, antioxidant, enzyme inhibitory, antimicrobial, and antimutagenic activities of leaf extracts from two selected cultivars in comparison with wild-growing plants have been evaluated. hplc-dad/esi-tof-ms analysis revealed the presence of phenolic acids and flavonoids with chlorogenic acid and rutin being the dominant compounds in the cultivated plants, whereas rutin and kaem ...201728095717
c-di-amp modulates listeria monocytogenes central metabolism to regulate growth, antibiotic resistance and osmoregulation.cyclic diadenosine monophosphate (c-di-amp) is a conserved nucleotide second messenger critical for bacterial growth and resistance to cell wall-active antibiotics. in listeria monocytogenes, the sole diadenylate cyclase, daca, is essential in rich, but not synthetic media and δdaca mutants are highly sensitive to the β-lactam antibiotic cefuroxime. in this study, loss of function mutations in the oligopeptide importer (oppabcdf) and glycine betaine importer (gbuabc) allowed δdaca mutants to gro ...201728097715
distinct neurotoxicity profile of listeriolysin o from listeria monocytogenes.cholesterol-dependent cytolysins (cdcs) are protein toxins that originate from gram-positive bacteria and contribute substantially to their pathogenicity. cdcs bind membrane cholesterol and build prepores and lytic pores. some effects of the toxins are observed in non-lytic concentrations. two pathogens, streptococcus pneumoniae and listeria monocytogenes, cause fatal bacterial meningitis, and both produce toxins of the cdc family-pneumolysin and listeriolysin o, respectively. it has been demons ...201728098781
the influence of the toxin/antitoxin mazef on growth and survival of listeria monocytogenes under stress.a major factor in the resilience of listeria monocytogenes is the alternative sigma factor b (σ(b)). type ii toxin/antitoxin (ta) systems are also known to have a role in the bacterial stress response upon activation via the clpp or lon proteases. directly upstream of the σ(b) operon in l. monocytogenes is the ta system mazef, which can cleave mrna at uacmu sites. in this study, we showed that the mazef ta locus does not affect the level of persister formation during treatment with antibiotics i ...201728098783
effect of humidity and temperature on the survival of listeria monocytogenes on surfaces.listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and raw & undercooked meats. stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface materials, temperature and relative humidity can affect survival of l. monocytogenes. in this study, survival of l. monocytogenes on stainless steel was investigated at three temperature ...201728101930
glutathione and glutathione transferase omega 1 as key posttranslational regulators in macrophages.macrophage activation during phagocytosis or by pattern recognition receptors, such as toll-like receptor 4, leads to the accumulation of reactive oxygen species (ros). ros act as a microbicidal defense mechanism, promoting clearance of infection, allowing for resolution of inflammation. overproduction of ros, however, overwhelms our cellular antioxidant defense system, promoting oxidation of protein machinery, leading to macrophage dysregulation and pathophysiology of chronic inflammatory condi ...201728102119
antimicrobial activity, ame resistance, and a-site binding studies of anthraquinone-neomycin conjugates.the antibacterial effects of aminoglycosides are based on their association with the a-site of bacterial rrna and interference with the translational process in the bacterial cell, causing cell death. the clinical use of aminoglycosides is complicated by resistance and side effects, some of which arise from their interactions with the human mitochondrial 12s rrna and its deafness-associated mutations, c1494u and a1555g. we report a rapid assay that allows screening of aminoglycoside compounds to ...201728103015
naturally occurring inlb variants that support intragastric listeria monocytogenes infection in mice.listeria monocytogenes is a causative agent of foodborne infection in humans and animals. the virulence factor inlb interacts with mammalian receptor c-met via its internalin domain to provide l. monocytogenes invasion in non-professional phagocytes. naturally occurring inlb internalin domain variants form four subclusters on the maximal likelihood tree. four variants belonging to distinct subclusters were cloned into the vector carrying 3΄ and 5΄-flanking sequences to restore full length inlb a ...201728104777
molecular characterization and functional analysis of a piscidin gene in large yellow croaker (larimichthys crocea).the piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. in this study, we cloned piscidin-5-like type 3 (lcpis5lt3) in large yellow croaker (larimichthys crocea). multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide (22 amino acids). the phylogenetic tree confirmed that lcpis5lt3 and large yellow croaker piscidin-5-l ...201628105799
listeria monocytogenes isolation from urine: a series of 15 cases and review. 201728110052
antibiotic discovery throughout the small world initiative: a molecular strategy to identify biosynthetic gene clusters involved in antagonistic activity.the emergence of bacterial pathogens resistant to all known antibiotics is a global health crisis. adding to this problem is that major pharmaceutical companies have shifted away from antibiotic discovery due to low profitability. as a result, the pipeline of new antibiotics is essentially dry and many bacteria now resist the effects of most commonly used drugs. to address this global health concern, citizen science through the small world initiative (swi) was formed in 2012. as part of swi, stu ...201728110506
an unusual presentation of listeria monocytogenes rhombencephalitis.we describe a case of 52-year-old woman with a medical history of crohn's disease presented abrupt fever, asymmetrical multiple cranial nerve palsies and focal neurological symptoms localized to the brainstem. the patient was initially diagnosed with ischaemic stroke, because of acute clinical course and results of neuroimaging. cerebrospinal fluid analysis revealed mild infection with negative gram staining and culture. final diagnosis of listeria monocytogenes brainstem infection (rhombencepha ...201728110853
l-glutamine induces expression of listeria monocytogenes virulence genes.the high environmental adaptability of bacteria is contingent upon their ability to sense changes in their surroundings. bacterial pathogen entry into host poses an abrupt and dramatic environmental change, during which successful pathogens gauge multiple parameters that signal host localization. the facultative human pathogen listeria monocytogenes flourishes in soil, water and food, and in ~50 different animals, and serves as a model for intracellular infection. l. monocytogenes identifies hos ...201728114430
characterization of class iia bacteriocin resistance in enterococcus faecium.vancomycin-resistant enterococci, particularly resistant enterococcus faecium, pose an escalating threat in nosocomial environments because of their innate resistance to many antibiotics, including vancomycin, a treatment of last resort. many class iia bacteriocins strongly target these enterococci and may offer a potential alternative for the management of this pathogen. however, e. faecium's resistance to these peptides remains relatively uncharacterized. here, we explored the development of r ...201728115354
use of rhodomyrtus tomentosa ethanolic leaf extract for the bio-control of listeria monocytogenes post-cooking contamination in cooked chicken meat.controlling foodborne pathogen in ready-to-eat food is important in food safety. the present study accessed the potential use of rhodomyrtus tomentosa ethanolic leaf extract as a bio-control agent against listeria monocytogenes in cooked chicken meat model system. the antilisterial activity of the plant extract was better under microwave condition and enhanced as storage temperature increased from 4 to 37 °c. the extract could reduce l. monocytogenes numbers at low (10(4) cfu/g) and high (10(6)  ...201628115764
prevalence and genetic characteristics of meatborne listeria monocytogenes isolates from livestock farms in korea.this study aimed to evaluate the prevalence of listeria monocytogenes on livestock farms in korea and determine their serotypes and genetic correlations. twenty-five livestock farms in korea (central: 15, south west: 7, south east: 3) were visited 2-3 times, and 2,018 samples (feces: 677, soil: 680, silage: 647, sludge: 14) were collected. samples were enriched in leb (listeria enrichment broth) and fraser broth media, and then plated on palcam agar. the isolates were identified by pcr and 16s r ...201628115889
antimicrobial activity of kefir against various food pathogens and spoilage bacteria.kefir is a unique fermented dairy product produced by a mixture of lactic acid bacteria, acetic acid bacteria, and yeast. here, we compared the antimicrobial spectra of four types of kefirs (a, l, m, and s) fermented for 24, 36, 48, or 72 h against eight food-borne pathogens. bacillus cereus, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, escherichia coli, salmonella enteritidis, pseudomonas aeruginosa, and cronobacter sakazakii were used as test strains, and antibacterial ...201628115890
evaluation of ta10 broth for recovery of listeria monocytogenes from ground beef.in 2009, the enrichment broth ta10 was released for simultaneous recovery of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7. this medium was compared with other salmonella enrichment broths [lactose (lac) broth, buffered peptone water (bpw), and universal pre-enrichment (up) broth] for the recovery of heat- and freeze-injured salmonella spp. in beef by the conventional culture method. there was a significant difference between ta10 and lac enrichment broths for detecting i ...201728118577
the role of universal stress proteins in bacterial infections.universal stress proteins are ubiquitously expressed in bacteria, archaea and plants and other eukaryotes. a general property of usps is their role in adaptation of bacteria to oxidative stress, high temperature, low ph and/or hypoxia. there is increasing evidence that these proteins facilitate the adaption of bacterial pathogens to the human host environment, thereby facilitating colonisation and pathogenicity. usps in mycobacterium tuberculosis are well studied and may play a role in latency o ...201728120710
stevia-based sweeteners as a promising alternative to table sugar: the effect on listeria monocytogenes and salmonella typhimurium growth dynamics.sugar is commonly substituted with stevia-based products in food industry and in our daily-life. this substitution results in a change in food product characteristic formula and properties that may affect the growth dynamics of food pathogenic and spoilage bacteria. this work studies the effect of table sugar (ts), laboratory sucrose (ls), commercial stevia (st) and steviol glycosides (sg) on the growth dynamics of salmonella typhimurium and listeria monocytogenes. experiments were carried out i ...201728126702
mathematical modelling of growth of listeria  monocytogenes in raw chilled pork.the aim of this study was to analyse the growth kinetics of listeria monocytogenes in naturally contaminated chilled pork. a cocktail of 26 meat-borne l. monocytogenes was inoculated to raw or sterile chilled pork to observe its growth at 4, 10, 16, 22 and 28°c respectively. the growth data were fitted by the baranyi model and ratkowsky square-root model. results showed that the baranyi model and ratkowsky square-root model could describe the growth characteristics of l. monocytogenes at differe ...201728129445
axonal invasion of listeria monocytogenes: implications for early diagnosis with magnetic resonance imaging. 201728131231
case report: when two and ½ men go camping….in patients, rapid identification of bacterial species may help to guide treatment at early stages. new protocols for the identification directly from positive blood culture flasks significantly helped in the presented case report.201728137306
activity of the pore-forming virulence factor listeriolysin o is reversibly inhibited by naturally occurring s-glutathionylation.cholesterol-dependent cytolysins (cdcs) represent a family of homologous pore-forming proteins secreted by many gram-positive bacterial pathogens. cdcs mediate membrane binding partly through a conserved c-terminal undecapeptide, which contains a single cysteine residue. while mutational changes to other residues in the undecapeptide typically have severe effects, mutation of the cysteine residue to alanine has minor effects on overall protein function. thus, the role of this highly conserved re ...201728138025
development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of listeria monocytogenes.listeria monocytogenes, one of most problematic foodborne pathogens, is responsible for listeriosis in both humans and animals and mainly transmitted through the food chain. in this report, we propose a simple, rapid, and nearly instrument-free molecular technique using multiple cross displacement amplification (mcda) label-based gold nanoparticles lateral flow biosensor (lfb) for specific, sensitive, and visual detection of l. monocytogenes. the mcda-lfb method was carried out at a constant tem ...201728138243
knowledge gaps in host-parasite interaction preclude accurate assessment of meat-borne exposure to toxoplasma gondii.toxoplasma gondii is recognized as a widely prevalent zoonotic parasite worldwide. although several studies clearly identified meat products as an important source of t. gondii infections in humans, quantitative understanding of the risk posed to humans through the food chain is surprisingly scant. while probabilistic risk assessments for pathogens such as campylobacter jejuni, listeria monocytogenes or escherichia coli have been well established, attempts to quantify the probability of human ex ...201628139250
diagnosis and treatment of listeria monocytogenes endophthalmitis: a systematic review.describe patient characteristics, treatment, and vision outcomes of listeria monocytogenes endophthalmitis, an exceedingly rare form of listeriosis.201728145786
diversity and persistence of listeria monocytogenes within the gorgonzola pdo production chain and comparison with clinical isolates from the same area.listeria monocytogenes causes invasive syndromes with high fatality rates in specific population groups. cheeses have been commonly implicated in outbreaks worldwide. gorgonzola is a cheese only produced in northwestern italy (it is the third italian cheese in terms of production and export) and l. monocytogenes is frequently isolated from the production chain. the aims of this study were to assess the distribution of l. monocytogenes virulence types (vts) in isolates collected in gorgonzola pro ...201728153561
phloretin attenuates listeria monocytogenes virulence both in vitro and in vivo by simultaneously targeting listeriolysin o and sortase a.the critical roles of sortase a (srta) and listeriolysin o (llo) in listeria monocytogenes pathogenicity render these two virulence factors as ideal targets for the development of anti-virulence agents against l. monocytogenes infection. additionally, the structures of srta and llo are highly conserved among the members of sortase enzyme family and cholesterol dependent toxin family. here, phloretin, a natural polyphenolic compound derived from apples and pears that has little anti-l. monocytoge ...201728154809
listeriolysin s: a bacteriocin from epidemic listeria monocytogenes strains that targets the microbiota.listeria monocytogenes is a gram-positive food-borne pathogen that in humans may traverse the intestinal, placental and blood/brain barriers, causing gastroenteritis, abortions and meningitis. crossing of these barriers is dependent on the bacterial ability to enter host cells, and several l. monocytogenes surface and secreted virulence factors are known to facilitate entry and the intracellular lifecycle. the study of l. monocytogenes strains associated to human listeriosis epidemics has reveal ...201728156183
survival of listeria monocytogenes with different antibiotic resistance patterns to food-associated stresses.the ongoing rise of antibiotic resistant microbial pathogens has become one of the major public health threats worldwide. despite all the effort and actions taken so far, a proliferation of antibiotic resistant (ar) and multi-antibiotic resistant (mar) strains is still observed, including in foodborne pathogens. this trend has been also noted recently for isolates of listeria monocytogenes, a species that, although remaining largely sensitive to clinically relevant antimicrobials, has been repor ...201728157581
synthesis, surfactant properties and antimicrobial activities of methyl glycopyranoside ethers.a series of amphiphilic methyl glucopyranoside ethers incorporating various alkyl chain lengths has been synthesized from commercially available methyl glucopyranosides following an acetalisation/hydrogenolysis sequence. the amphiphilic properties of ethers and acetal intermediates were evaluated. both families exhibit excellent surfactant properties with a maximum efficiency obtained for compounds bearing a linear dodecyl chain (cmc = 0.012 mm, γsat. = 30 mn m(-1)). antimicrobial activity studi ...201728157594
hot topic: antilisterial activity by endolysin plyp100 in fresh cheese.our objective was to assess the antimicrobial efficacy of a listeria bacteriophage endolysin that may address limitations of current antilisterial processes for fresh cheeses. listeria monocytogenes is highly problematic in the manufacture and processing of ready-to-eat foods due to its environmental persistence and its ability to grow under refrigerated storage. special care must be taken to prevent listerial contamination during the production of fresh cheeses, as their delicate flavor and tex ...201728161174
activation and exhaustion of antigen-specific cd8(+) t cells occur in different splenic compartments during infection with plasmodium berghei.the spleen is the major organ in which t cells are primed during infection with malaria parasites. however, little is known regarding the dynamics of the immune responses and their localization within the splenic tissue during malaria infection. we examined murine cd8(+) t cell responses during infection with plasmodium berghei using recombinant parasites expressing a model antigen ovalbumin (ova) protein and compared the responses with those elicited by listeria monocytogenes expressing the sam ...201728163249
assessing the genome level diversity of listeria monocytogenes from contaminated ice cream and environmental samples linked to a listeriosis outbreak in the united states.a listeriosis outbreak in the united states implicated contaminated ice cream produced by one company, which operated 3 facilities. we performed single nucleotide polymorphism (snp)-based whole genome sequencing (wgs) analysis on listeria monocytogenes from food, environmental and clinical sources, identifying two clusters and a single branch, belonging to pcr serogroup iib and genetic lineage i. wgs cluster i, representing one outbreak strain, contained 82 food and environmental isolates from f ...201728166293
rhombencephalitis caused by listeria monocytogenes in a pastured bull.a pastured 2-y-old cross-breed bull developed brainstem encephalitis (rhombencephalitis); listeria monocytogenes was isolated from the brain. in the brainstem, there was perivascular cuffing, multiple microabscesses, and positive immunostaining for l. monocytogenes. samples of bovine feces, water, feedstuffs, milking parlor soil, and bulk tank milk were collected from the dairy farm. seven isolates of the genus listeria were obtained, 6 of l. innocua and 1 of l. monocytogenes, which was found in ...201728166691
comparative analysis of the bacterial membrane disruption effect of two natural plant antimicrobial peptides.in the medicago truncatula genome about 700 genes code for nodule-specific cysteine-rich (ncr) small peptides that are expressed in the symbiotic organ, the root nodule, where they control terminal differentiation of the endosymbiotic rhizobium bacteria to nitrogen-fixing bacteroids. cationic ncr peptides were predicted to have antimicrobial activities. here antibacterial activities of ncr247, ncr335, polymyxin b (pmb), and streptomycin were investigated and compared on two foodborne pathogens s ...201728167938
100k pathogen genome project: 306 listeria draft genome sequences for food safety and public health.listeria monocytogenes is a food-associated bacterium that is responsible for food-related illnesses worldwide. this is the initial public release of 306 l. monocytogenes genome sequences as part of the 100k pathogen genome project. these isolates represent global genomic diversity in l. monocytogenes.201728183778
seeds of the wild progenitor of maize possess bacteria that antagonize foodborne pathogens.endophytes are microorganisms that inhabit plant tissues without causing disease. some endophytes help their hosts to combat pathogens. here we explored the hypothesis that the plant-derived foods consumed by humans and other animals host endophytes that also antagonize foodborne pathogens or food-rotting agents. our laboratory previously cultured a library of bacterial endophytes from different members of the maize/corn family (zea) including wild relatives. here, 190 of these endophytes were s ...201728186422
32-phosphorus selectively delivered by listeria to pancreatic cancer demonstrates a strong therapeutic effect.our laboratory has developed a novel delivery platform using an attenuated non-toxic and non-pathogenic bacterium listeria monocytogenes that infects tumor cells and selectively survives and multiplies in metastases and primary tumors with help of myeloid-derived suppressor cells (mdsc) and immune suppression in the tumor microenvironment (tme). 32p was efficiently incorporated into the listeria bacteria by starvation of the bacteria in saline, and then cultured in phosphorus-free medium complem ...201728186976
isolation and identification of a novel bacterium, lactobacillus sakei subsp. dgh strain 5, and optimization of growth condition for highest antagonistic activity.in the present study, we isolated lactobacillus sakei strain dgh5 from raw beef meat. this bacterium plays an inhibitory effect against food-spoiling bacteria and food-borne pathogens, including listeria monocytogenes, a gram-positive and pathogenic bacterium. lactobacillus sakei strain dgh5 was identified through both phenotypical and biochemical tests accompanied with 16s rrna sequence analysis. among all the sources of carbon, nitrogen and phosphorous forms, we selected the most potent compou ...201728188900
invited review: hispanic-style cheeses and their association with listeria monocytogenes.the rise in consumption of hispanic-style cheeses (hsc), due in large part to the increasing hispanic population in the united states, has not been met with advances in food safety sufficient to prevent the numerous outbreaks and recalls due to listeria monocytogenes. hispanic-style cheeses are typically high moisture and have low salt content and low acidity from being subjected to little to no ripening. these conditions necessitate refrigeration to maintain safety and quality, as the majority ...201728189316
novel bacteriocinogenic enterococcus hirae and pediococcus pentosaceus strains with antilisterial activity isolated from brazilian artisanal cheese.we isolated and characterized bacteriocin producers enterococcus hirae st57acc and pediococcus pentosaceus st65acc from raw milk artisanal cheeses. their bacteriocins were tolerant to temperatures from 4°c to 100°c and under sterilization conditions (121°c for 15 min). additionally, the tested bacteriocins remained active after being exposed to ph 2.0 to 10.0 for 2 h. the activity of the bacteriocins was affected by proteolytic enzymes but remained stable after treatment with edta, sodium dodecy ...201728189328
assessing the capacity of growth, survival, and acid adaptive response of listeria monocytogenes during storage of various cheeses and subsequent simulated gastric digestion.different physicochemical and microbiological characteristics of cheeses may affect listeria monocytogenes potential to grow, survive, or exhibit an acid adaptive response during storage and digestion. the objectives of the present study were to assess: i) the survival or growth potential of l.monocytogenes on various cheeses during storage, ii) the effect of initial indigenous microbiota on pathogen growth in comparison to expected growth curves retrieved by existing predictive models, and iii) ...201728189900
an assessment of the microbiological quality of liver-based pâté in england 2012-13: comparison of samples collected at retail and from catering businesses.the purpose of this study was to investigate the microbiological quality of liver pâté. during 2012-13, a total of 870 samples, unrelated to the investigation of food-poisoning outbreaks, were collected either at retail (46%), catering (53%) or the point of manufacture (1%) and were tested using standard methods to detect salmonella spp. or campylobacter spp., and to enumerate for listeria spp., including listeria monocytogenes, clostridium perfringens, coagulase-positive staphylococci including ...201728190406
n-terminomics identifies prli42 as a membrane miniprotein conserved in firmicutes and critical for stressosome activation in listeria monocytogenes.to adapt to changing environments, bacteria have evolved numerous pathways that activate stress response genes. in gram-positive bacteria, the stressosome, a cytoplasmic complex, relays external cues and activates the sigma b regulon. the stressosome is structurally well-characterized in bacillus, but how it senses stress remains elusive. here, we report a genome-wide n-terminomic approach in listeria that strikingly led to the discovery of 19 internal translation initiation sites and 6 miniprot ...201728191904
a redox-responsive transcription factor is critical for pathogenesis and aerobic growth of listeria monocytogenes.bacterial pathogens have evolved sophisticated mechanisms to sense and adapt to redox stress in nature and within the host. however, deciphering the redox environment encountered by intracellular pathogens in the mammalian cytosol is challenging and remains poorly understood. in this study, we assessed the contributions of the two redox-responsive, spx-family transcriptional regulators to the virulence of listeria monocytogenes, a gram-positive facultative intracellular pathogen. spx-family prot ...201728193635
bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides.several products of industrial interest are produced by bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. this study aimed to investigate the potential of two bacterial isolates (p5 and c3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. phylogenetic analyses showed close relation of isolates p5 and c3 with bacillus amyloliquefaciens and bacillus thuringie ...201728197131
sumoylation as an integral mechanism in bacterial infection and disease progression.post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. among these, particularly the modification with small ubiquitin-like modifiers (sumo) is being recognized as a pathway crucial for cell homeostasis and health. understandably, bacterial pathogens intervene with the sumo pathway of the host for ensuring successful infection. among the bacterial pathogens known to target host sumoylation varied points of intervention are utilized. majo ...201728197924
attachment of 13 types of foodborne bacteria to jalapeño and serrano peppers and antibacterial effect of roselle calyx extracts, sodium hypochlorite, colloidal silver, and acetic acid against these foodborne bacteria on peppers.chili peppers are a very important crop in mexico. however, these peppers have been associated with salmonella infection outbreaks in the united states, and salmonella and diarrheagenic escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in mexico. to decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. roselle ( h ...201728199144
morphological change and decreasing transfer rate of biofilm-featured listeria monocytogenes egde.listeria monocytogenes , a lethal foodborne pathogen, has the ability to resist the hostile food processing environment and thus frequently contaminates ready-to-eat foods during processing. it is commonly accepted that the tendency of l. monocytogenes ' to generate biofilms on various surfaces enhances its resistance to the harshness of the food processing environment. however, the role of biofilm formation in the transferability of l. monocytogenes egde remains controversial. we examined the g ...201728199146
diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on listeria monocytogenes and salmonella enterica serovar enteritidis in a co-culture model.food-borne infections cause huge economic and human life losses. listeria monocytogenes and salmonella enterica serovar enteritidis are among the top ranking pathogens causing such losses. control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to ...201728202007
requirement of the rna-binding protein smpb during intracellular growth of listeria monocytogenes.bacterial trans-translation is the main quality control mechanism employed to relieve stalled ribosomes. trans-translation is mediated by the small protein b (smpb) and transfer-mrna (tmrna) ribonucleoprotein complex, which interacts with translational complexes stalled at the 3' end of non-stop mrnas to release the stalled ribosomes thereby targeting the nascent polypeptides and truncated mrnas for degradation. the trans-translation system exists with a few exceptions in all bacteria. in the pr ...201728202229
evaluation of the effect of radiofrequency radiation emitted from wi-fi router and mobile phone simulator on the antibacterial susceptibility of pathogenic bacteria listeria monocytogenes and escherichia coli.mobile phones and wi-fi radiofrequency radiation are among the main sources of the exposure of the general population to radiofrequency electromagnetic fields (rf-emf). previous studies have shown that exposure of microorganisms to rf-emfs can be associated with a wide spectrum of changes ranged from the modified bacterial growth to the alterations of the pattern of antibiotic resistance. our laboratory at the nonionizing department of the ionizing and non-ionizing radiation protection research ...201728203122
tim-3 inhibits macrophage control of listeria monocytogenes by inhibiting nrf2.t cell immunoglobulin mucin-3 (tim-3) is an immune checkpoint inhibitor and its dysregulation has been related to t cell tolerance and many immune disorders, such as tumors and infection tolerance. however, the physiopathology roles of tim-3 in innate immunity remain elusive. here, we demonstrate that tim-3 inhibits macrophage phagocytosis of l. monocytogenes by inhibiting the nuclear erythroid 2-related factor 2 (nrf2) signaling pathway and increases bacterial burden. tim-3 signaling promotes n ...201728205579
nanoengineered eggshell-silver tailored copolyester polymer blend film with antimicrobial properties.in this study, the reinforcement effect of different proportions of eggshell/silver (es-ag) nanomaterial on the structural and antimicrobial properties of 70/30 poly(butylene-co-adipate terephthalate)/polylactic acid (pbat/pla) immiscible blends was investigated. the es-ag was synthesized using a single step ball milling process and characterized with x-ray diffraction (xrd), x-ray photoelectron spectroscopy (xps), and transmission electron microscopy (tem). these results confirmed the existence ...201728206760
growth of listeria monocytogenes in thawed frozen foods.the growth characteristics of listeria monocytogenes inoculated onto frozen foods (corn, green peas, crabmeat, and shrimp) and thawed by being stored at 4, 8, 12, and 20°c were investigated. the growth parameters, lag-phase duration (lpd) and exponential growth rate (egr), were determined by using a two-phase linear growth model as a primary model and a square root model for egr and a quadratic model for lpd as secondary models, based on the growth data. the egr model predictions were compared w ...201728207303
prevalence of listeria monocytogenes in retail lightly pickled vegetables and its successful control at processing plants.incidences of food poisoning traced to nonanimal food products have been increasingly reported. one of these was a recent large outbreak of shiga toxin-producing escherichia coli (stec) o157 infection from the consumption of lightly pickled vegetables, indicating the necessity of imposing hygienic controls during manufacturing. however, little is known about the bacterial contamination levels in these minimally processed vegetables. here we examined the prevalence of stec, salmonella spp., and l ...201728207308
yope specific cd8+ t cells provide protection against systemic and mucosal yersinia pseudotuberculosis infection.prior studies indicated that cd8+ t cells responding to a surrogate single antigen expressed by y. pseudotuberculosis, ovalbumin, were insufficient to protect against yersiniosis. herein we tested the hypothesis that cd8+ t cells reactive to the natural yersinia antigen yope would be more effective at providing mucosal protection. we first confirmed that immunization with the attenuated ksga- strain of y. pseudotuberculosis generated yope-specific cd8+ t cells. these t cells were protective agai ...201728207901
assessment of the antimicrobial activity of olive leaf extract against foodborne bacterial pathogens.olive leaf extract (ole) has been used traditionally as a herbal supplement since it contains polyphenolic compounds with beneficial properties ranging from increasing energy levels, lowering blood pressure, and supporting the cardiovascular and immune systems. in addition to the beneficial effects on human health, ole also has antimicrobial properties. the aim of this work was to investigate the antimicrobial effect of ole against major foodborne pathogens, including listeria monocytogenes, esc ...201728210244
inactivation of salmonella spp., pathogenic escherichia coli, staphylococcus spp., or listeria monocytogenes in chicken purge or skin using a 405-nm led array.raw poultry are sometimes contaminated with foodborne pathogens, which can lead to illness in humans. in recent years research has focused on a variety of light technologies to decontaminate food and food contact surfaces during meat and poultry processing. in this study we evaluated the ability of 405-nm light generated from an led array to inactivate multi-isolate cocktails of either salmonella spp., pathogenic escherichia coli, staphylococcus spp., or listeria monocytogenes suspended in chick ...201728213017
pathogen biofilm formation on cantaloupe surface and its impact on the antibacterial effect of lauroyl arginate ethyl.pathogen biofilm at fruit surface may pose a particular risk to food safety. in this study, the biofilms of listeria monocytogenes v7 and salmonella enterica serovar typhimurium atcc 13311 on cantaloupe fruit surface were visualized, and the resistance of biofilms against lauroyl arginate ethyl (lae, an antibacterial compound) was evaluated. each bacterium was inoculated on isolated cantaloupe rind surfaces at 10(5)-10(6) cfu/cm(2) and after incubation for 2, 12, 24, and 48 h, the surfaces were ...201728213018
growth interactions and antilisterial effects of the bacteriocinogenic lactococcus lactis subsp. cremoris m104 and enterococcus faecium ke82 strains in thermized milk in the presence or absence of a commercial starter culture.traditional greek cheeses are often produced from thermized milk (tm) with the use of commercial starter cultures (cscs), which may not inhibit growth of listeria monocytogenes completely. therefore, this study evaluated the behavior of an artificial l. monocytogenes contamination in commercially tm (63 °c; 30 s) inoculated with a csc plus lactococcus lactis subsp. lactis m104 and/or enterococcus faecium ke82, two indigenous strains producing nisin a and enterocin a and b, respectively. inoculat ...201728213019
prevalence, genetic diversity and antimicrobial resistance of listeria monocytogenes isolated from fresh and smoked fish in poland.a total of 57 out of 301 (18.9%) fresh and smoked fish samples in poland were positive for listeria monocytotgenes. the bacteria were most frequently identified in fresh and smoked salmon (32.0% and 33.8% respectively) as well as in fresh cod (31.8%). only three samples of smoked salmon were contaminated with the bacteria above 100 cfu/g. four molecular serogroups were identified and the most prevalent, 1/2a-3a (40 isolates; 70.2%), was present in samples from both marine (33 strains; 71.7%) and ...201728213022
inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in ready-to-bake cookie dough by gamma and electron beam irradiation.this study was conducted to investigate the efficacy of gamma and electron beam irradiation to inactivate foodborne pathogens in ready-to-bake cookie dough and to determine the effect on quality by measuring color and texture changes. cookie dough inoculated with escherichia coli o157:h7, salmonella typhimurium, or listeria monocytogenes was subjected to gamma and electron beam irradiation, with doses ranging from 0 to 3 kgy. as the radiation dose increased, the inactivation effect increased amo ...201728213023
high hydrostatic pressure effects on listeria monocytogenes and l. innocua: evidence for variability in inactivation behaviour and in resistance to pediocin bacha-6111-2.the effect of high hydrostatic pressure (hhp) on the survival of 14 strains of listeria monocytogenes from food or clinical origins, selected to represent different pheno and genotypes, was evaluated. stationary phase cells were submitted to 300, 400 and 500 mpa at 10 °c, for 5 min. a high variability in the resistance of l. monocytogenes to pressure was observed, and particularly two strains isolated from food were significantly more baroresistant than the rest. strains of l. monocytogenes resi ...201728213030
antimicrobial treatments to control listeria monocytogenes in queso fresco.queso fresco, is a hispanic non-fermented cheese highly susceptible to contamination with l. monocytogenes. this research was aimed to determine the effect of gras antimicrobial ingredients to control l. monocytogenes. antimicrobials included caprylic acid (ca), nisaplin(®) (n, 2.5% nisin), a mixture of sodium lactate and sodium diacetate (sl/sd), lactococcus lactis sbp. lactis dpc 3147, monolaurin, and lactic acid (la). batches of queso fresco curds were inoculated with 10(4) cfu/g and stored a ...201728213034
influence of electro-activated solutions of weak organic acid salts on microbial quality and overall appearance of blueberries during storage.the aim of this work was to study the potential of diluted electro-activated solutions of weak organic acid salts (potassium acetate, potassium citrate and calcium lactate) to extend the shelf life of blueberries during post-harvest storage. the sanitizing capacity of these solutions was studied against pathogenic bacteria listeria monocytogenes and e. coli o157:h7 as well as phytopathogenic fungi a. alternata, f. oxysporum and b. cinerea. the results showed that a 5-min treatment of inoculated ...201728213035
monocytes are the predominant cell type associated with listeria monocytogenes in the gut, but they do not serve as an intracellular growth niche.after foodborne transmission of the facultative intracellular bacterial pathogen listeria monocytogenes, most of the bacterial burden in the gut is extracellular. however, we previously demonstrated that intracellular replication in an as yet unidentified cell type was essential for dissemination and systemic spread of l. monocytogenes in this article, we show that the vast majority of cell-associated l. monocytogenes in the gut were adhered to ly6c(hi) monocytes, a cell type that inefficiently ...201728213502
the effects of atmospheric pressure cold plasma treatment on microbiological, physical-chemical and sensory characteristics of vacuum packaged beef loin.effects on vacuum packaged and non-packaged beef longissimus samples exposed to atmospheric cold plasma (acp) generated at different powers were studied over a 10day period of vacuum-, and a subsequent 3day period of aerobic storage. exposure of non-covered beef samples under high power acp conditions resulted in increased a*, b*, chroma and hue values, but acp treatment of packaged loins did not impact colour (l*, a*, b*, chroma, hue), lipid peroxidation, sarcoplasmic protein denaturation, nitr ...201728214695
microbiological quality of pastrami and associated surfaces at the point of sale in kayseri, turkey.the aim of this study is to trace the possible relations between the hygienic status of slicing utensils and the microbiological quality of pastrami.201728216007
listeria monocytogenes 10403s arginine repressor argr finely tunes arginine metabolism regulation under acidic conditions.listeria monocytogenes is able to colonize human and animal intestinal tracts and to subsequently cross the intestinal barrier, causing systemic infection. for successful establishment of infection, l. monocytogenes must survive the low ph environment of the stomach. l. monocytogenes encodes a functional argr, a transcriptional regulator belonging to the argr/ahrc arginine repressor family. we aimed at clarifying the specific functions of argr in arginine metabolism regulation, and more importan ...201728217122
environmental occurrence and antibiotic susceptibility profile of listeria monocytogenes at a slaughterhouse raw processing plant in romania.this survey was conducted to investigate the occurrence and antimicrobial susceptibility of listeria monocytogenes isolates in the environment of a pig slaughterhouse raw processing plant complex in western romania. a total of 97 environmental samples from food contact (n = 60) and nonfood contact (n = 37) surfaces were examined with standard methods. the susceptibility of the isolates to 17 antimicrobial agents was determined with the vitek 2 automated system. overall, 25 (25.8%) samples were p ...201628221837
fate of pathogenic bacteria associated with production of pickled sausage by using a cold fill process.preservation by pickling has been used for many years to extend the shelf life of various types of food products. by storing meat products in a brine solution containing an organic acid, salt, spices, as well as other preservatives, the ph of the product is reduced, thus increasing the safety and shelf life of the product. pickling may involve the use of heated brines to further add to the safety of the food product. when precooked, ready-to-eat (rte) sausages are pickled with a heated brine sol ...201628221846
listeria monocytogenes isolates carrying virulence-attenuating mutations in internalin a are commonly isolated from ready-to-eat food processing plant and retail environments.listeria monocytogenes is a human foodborne pathogen that may cause an invasive disease known as listeriosis in susceptible individuals. internalin a (inla; encoded by inla) is a virulence factor that facilitates crossing of host cell barriers by l. monocytogenes . at least 19 single nucleotide polymorphisms (snps) in inla that result in a premature stop codon (pmsc) have been described worldwide. snps leading to a pmsc in inla have been shown to be causally associated with attenuated virulence. ...201628221857
prediction of escherichia coli o157:h7, salmonella , and listeria monocytogenes growth in leafy greens without temperature control.a recent study by the centers for disease control and prevention reported that between 1998 and 2008, leafy greens outbreaks accounted for 22.3% of foodborne outbreaks in the united states. several studies on the growth of bacteria at different temperatures have been conducted; however, there is a need for the prediction of bacterial growth when leafy greens are transported without temperature control. food products, when taken out of refrigeration, undergo a temperature change, with the rate of ...201728221878
microbial performance of food safety control and assurance activities in a fresh produce processing sector measured using a microbial assessment scheme and statistical modeling.current approaches such as inspections, audits, and end product testing cannot detect the distribution and dynamics of microbial contamination. despite the implementation of current food safety management systems, foodborne outbreaks linked to fresh produce continue to be reported. a microbial assessment scheme and statistical modeling were used to systematically assess the microbial performance of core control and assurance activities in five kenyan fresh produce processing and export companies ...201728221882
enrichment, amplification, and sequence-based typing of salmonella enterica and other foodborne pathogens.detection of salmonella enterica in foods typically involves microbiological enrichment, molecular-based assay, and subsequent isolation and identification of a pure culture. this is ideally followed by strain typing, which provides information critical to the investigation of outbreaks and the attribution of their sources. pulsed-field gel electrophoresis is the "gold standard" for s. enterica strain typing, but its limitations have encouraged the search for alternative methods, including whole ...201728221883
enhanced control of listeria monocytogenes by enterococcus faecium ke82, a multiple enterocin-producing strain, in different milk environments.enterococcus faecium ke82, isolated from traditional greek graviera cheese, was identified in pure broth cultures in vitro as a multiple enterocin-producing bacterial strain possessing the structural enta, entb, and entp enterocin genes. e. faecium ke82 was further assessed for in situ antilisterial activity in raw milk (rm) and commercially thermized milk (tm; 63°c for 30 s) in the presence of the indigenous microbiota and in sterile raw milk (srm; 121°c for 5 min) with or without the addition ...201728221890
microbial quality, safety, and pathogen detection by using quantitative pcr of raw salad vegetables sold in dhanbad city, india.consumption of ready-to-eat fresh vegetables has increased worldwide, with a consequent increase in outbreaks caused by foodborne pathogens. in the indian subcontinent, raw fresh vegetables are usually consumed without washing or other decontamination procedures, thereby leading to new food safety threats. in this study, the microbiological quality and pathogenic profile of raw salad vegetables was evaluated through standard protocols. in total, 480 samples (60 each of eight different salad vege ...201728221891
prevalence and persistence of listeria monocytogenes in ready-to-eat tilapia sashimi processing plants.a 2-year study was performed at two ready-to-eat tilapia sashimi processing plants (a and b) to identify possible routes of contamination with listeria monocytogenes during processing. samples were collected from the aquaculture environments, transportation tanks, processing plants, and final products. seventy-nine l. monocytogenes isolates were found in the processing environments and final products; 3.96% (50 of 1,264 samples) and 3.86% (29 of 752 samples) of the samples from plants a and b, r ...201628221901
prevalence and level of listeria monocytogenes in ice cream linked to a listeriosis outbreak in the united states.a most-probable-number (mpn) method was used to enumerate listeria monocytogenes in 2,320 commercial ice cream scoops manufactured on a production line that was implicated in a 2015 listeriosis outbreak in the united states. the analyzed samples were collected from seven lots produced in november 2014, december 2014, january 2015, and march 2015. l. monocytogenes was detected in 99% (2,307 of 2,320) of the tested samples (lower limit of detection, 0.03 mpn/g), 92% of which were contaminated at < ...201628221903
effect of temperature and nutrient concentration on survival of foodborne pathogens in deciduous fruit processing environments for effective hygiene management.temperature and good sanitation practices are important factors for controlling growth of microorganisms. fresh produce is stored at various temperatures to ensure quality and to prolong shelf life. when foodborne pathogens survive and grow on fresh produce at storage temperatures, then additional control strategies are needed to inactivate these pathogens. the aim of this study was to determine how temperatures associated with deciduous fruit processing and storage facilities (0.5, 4, and 21°c) ...201628221909
contribution of selective conditions to microbial competition in four listeria selective enrichment formulations.microbial competition during selective enrichment negatively affects listeria monocytogenes populations and may hinder the subsequent detection or recovery of this organism. competition assays among 10 selected strains of listeria and citrobacter braakii were performed in buffered listeria enrichment broth, 3-(n-morpholino)propanesulfonic acid-buffered listeria enrichment broth, university of vermont medium-modified listeria enrichment broth, and fraser broth. the individual contributions of eac ...201628221922
effect of power levels on inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in tomato paste using 915-megahertz microwave and ohmic heating.the effect of power levels on inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in tomato paste was investigated using 915-mhz microwave heating (mw) and ohmic heating (oh). heating uniformity, pathogen inactivation, and quality aspects were determined with 1.8-, 2.1-, 2.4-, and 3.0-kw mw and corresponding oh. ginafit was used to analyze pathogen inactivation. the heating uniformity of mw-treated samples was inferior to that of oh-treated samples at low ...201628221946
synergistic antilisterial effects of mixtures of lysozyme and organic acids.we investigated the synergistic effects of lysozyme combined with organic acids to inhibit the growth of listeria monocytogenes . the antilisterial effects of the combination of lysozyme and acetic acid, citric acid, lactic acid, malic acid, or succinic acid were evaluated using the checkerboard method and time-kill assay. the mic was 25,000 mg/liter for lysozyme, 625 mg/liter for acetic acid, and 1,250 mg/liter for the other acids. the mbc was 10,000 mg/liter for all of the tested organic acids ...201628221961
development and validation of pathogen environmental monitoring programs for small cheese processing facilities.pathogen environmental monitoring programs (emps) are essential for food processing facilities of all sizes that produce ready-to-eat food products exposed to the processing environment. we developed, implemented, and evaluated emps targeting listeria spp. and salmonella in nine small cheese processing facilities, including seven farmstead facilities. individual emps with monthly sample collection protocols were designed specifically for each facility. salmonella was detected in only one facilit ...201628221969
fate of listeria monocytogenes , pathogenic yersinia enterocolitica , and escherichia coli o157:h7 gfp(+) in ready-to-eat salad during cold storage: what is the risk to consumers?in this study, we investigated the fate of listeria monocytogenes , pathogenic yersinia enterocolitica , and escherichia coli o157:h7 gfp(+) inoculated in low numbers into ready-to-eat baby spinach and mixed-ingredient salad (baby spinach with chicken meat). samples were stored at recommended maximum refrigerator temperature (8°c in sweden) or at an abuse temperature (15°c) for up to 7 days. mixed-ingredient salad supported considerable growth when stored at 15°c during shelf life (3 days), with ...201728221975
listeria monocytogenes in smoked salmon and other smoked fish at retail in italy: frequency of contamination and strain characterization in products from different manufacturers.seven hundred seventy-eight samples of packaged smoked fish (774 smoked salmon and 4 smoked swordfish) on sale in italy, from 50 different manufacturers located in 12 european union countries, were purchased from the italian market between may and december 2011. the surface temperatures of the samples on sale ranged from 0 to 13°c (3.4 ± 1.5°c, mean ± sd). six hundred eighty (87.4%) of 778 samples were stored at ≤4°c. one hundred fifty-seven samples (20.2%, 95% confidence interval 17.5 to 23.1%) ...201728221976
acute fetal demise with first trimester maternal infection resulting from listeria monocytogenes in a nonhuman primate model.infection with listeria monocytogenes during pregnancy is associated with miscarriage, preterm birth, and neonatal complications, including sepsis and meningitis. while the risk of these conditions is thought to be greatest during the third trimester of pregnancy, the determinants of fetoplacental susceptibility to infection, the contribution of gestational age, and the in vivo progression of disease at the maternal-fetal interface are poorly understood. we developed a nonhuman primate model of ...201728223455
determination of antibacterial and technological properties of vaginal lactobacilli for their potential application in dairy products.functional foods could differently affect human health in relation to the gender. recent studies have highlighted the anti-candida and anti-chlamydia activities of some lactobacillus strains isolated from the vagina of healthy women. considering these important beneficial activities on women's health, the preparation of functional food containing active vaginal lactobacilli can represent a great scientific challenge for the female gender. in this context, the aim of this work was to study some f ...201728223974
structural characterization and in vitro fermentation of β-mannooligosaccharides produced from locust bean gum by gh-26 endo-β-1,4-mannanase (manb-1601).size exclusion chromatography of β-mannooligosaccharides (β-mos) mixtures, obtained from manb-1601 hydrolysis of locust bean gum, resulted in separation of oligosaccharides with various degrees of polymerization (dp 2, 3, and 5). the oligosaccharides were structurally [esi-ms, ftir, xrd, tga, and nmr ((1)h and (13)c)] and functionally (in vitro fermentation) characterized. dp2 oligosaccharide was composed of two species, (a) mannopyranose β-1,4 mannopyranose and (b) α-1,6-galactosyl-mannopyranos ...201728225615
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