Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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closing the brief case: neonatal meningitis caused by listeria monocytogenes diagnosed by multiplex molecular panel. | 2016 | 27881637 | |
cationic nanostructures against foodborne pathogens. | 2016 | 27881979 | |
immune signatures of protective spleen memory cd8 t cells. | memory cd8 t lymphocyte populations are remarkably heterogeneous and differ in their ability to protect the host. in order to identify the whole range of qualities uniquely associated with protective memory cells we compared the gene expression signatures of two qualities of memory cd8 t cells sharing the same antigenic-specificity: protective (influenza-induced, flu-tm) and non-protective (peptide-induced, tim) spleen memory cd8 t cells. although flu-tm and tim express classical phenotypic memo ... | 2016 | 27883012 |
antimicrobial activity and bioguided fractionation of rumex tingitanus extracts for meat preservation. | this study was undertaken to investigate the antibacterial and antifungal activities of rumex tingitanus leaves extracts as well as the identification of bioactive components and their performance in meat preservation. total phenolics and flavonoids showed the highest content of phenolics and flavonoids in the ethyl acetate fraction (rt etoacf). for antimicrobial efficacy, leaves extract and derived fraction were tested for their capacity to inhibit bacterial and fungal proliferation in vitro an ... | 2017 | 27883958 |
inhibition of listeriolysin o oligomerization by lutein prevents listeria monocytogenes infection. | the foodborne pathogenic bacterial species listeria monocytogenes (l. monocytogenes) has caused incalculable damages to public health, and its successful infection requires various virulence factors, including listeriolysin o (llo). by forming pores in phagosomal membranes and even in some organelles, llo plays an indispensable role in the ability of l. monocytogenes to escape from host immune attacks. because of its critical role, llo offers an appropriate therapeutic target against l. monocyto ... | 2017 | 27884571 |
the microbiota of marketed processed edible insects as revealed by high-throughput sequencing. | entomophagy has been linked to nutritional, economic, social and ecological benefits. however, scientific studies on the potential safety risks in eating edible insects need to be carried out for legislators, markets and consumers. in this context, the microbiota of edible insects deserves to be deeply investigated. the aim of this study was to elucidate the microbial species occurring in some processed marketed edible insects, namely powdered small crickets, whole dried small crickets (acheta d ... | 2017 | 27889142 |
antimicrobial activity of whey protein films supplemented with lactobacillus sakei cell-free supernatant on fresh beef. | the aim of this work was to evaluate the antimicrobial activity of whey protein isolate (wpi) films supplemented with lactobacillus sakei nrrl b-1917 cell-free supernatant on beef inoculated with escherichia coli atcc 25922 or listeria monocytogenes scott a; additionally, sensory evaluation was performed on wrapped beef cubes. supernatant concentrates were obtained from lb. sakei cultures in mrs broth after centrifugation, filtering, and freeze-drying. films were prepared with wpi (3% w/w), algi ... | 2017 | 27889150 |
fat and fibre interfere with the dramatic effect that nanoemulsified d-limonene has on the heat resistance of listeria monocytogenes. | the application of d-limonene in form of nanoemulsion has been proved to reduce dramatically the thermal resistance of listeria monocytogenes in culture media. the present research shows very promising results on the application in food products. the thermal resistance of l. monocytogenes was reduced 90 times when 0.5 mm nanoemulsified d-limonene was added to apple juice. this is the biggest reduction in the heat resistance of a microorganism caused by an antimicrobial described ever. however, n ... | 2017 | 27889158 |
exposure to minimally processed pear and melon during shelf life could modify the pathogenic potential of listeria monocytogenes. | survival and virulence of foodborne pathogens can be influenced by environmental factors such as the intrinsic properties of food as well as the extrinsic properties that contribute to food shelf life (e.g., temperature and gas atmosphere). the direct contribution of food matrix characteristics on the survival of l. monocytogenes during fresh-cut fruit shelf life is not very well understood. in addition, the gastrointestinal tract is the primary route of listeriosis infection and penetration of ... | 2017 | 27889159 |
effect of inoculum size, bacterial species, type of surfaces and contact time to the transfer of foodborne pathogens from inoculated to non-inoculated beef fillets via food processing surfaces. | the objective of the present study was to determine the factors affecting the transfer of foodborne pathogens from inoculated beef fillets to non-inoculated ones, through food processing surfaces. three different levels of inoculation of beef fillets surface were prepared: a high one of approximately 10(7) cfu/cm(2), a medium one of 10(5) cfu/cm(2) and a low one of 10(3) cfu/cm(2), using mixed-strains of listeria monocytogenes, or salmonella enterica typhimurium, or escherichia coli o157:h7. the ... | 2017 | 27889165 |
septins and bacterial infection. | septins, a unique cytoskeletal component associated with cellular membranes, are increasingly recognized as having important roles in host defense against bacterial infection. a role for septins during invasion of listeria monocytogenes into host cells was first proposed in 2002. since then, work has shown that septins assemble in response to a wide variety of invasive bacterial pathogens, and septin assemblies can have different roles during the bacterial infection process. here we review the i ... | 2016 | 27891501 |
microbiology of processed edible insect products - results of a preliminary survey. | little is known of the microbiology of processed insect products. the present survey analysed a total of n=38 samples of deep-fried and spiced (acheta domesticus, locusta migratoria, and omphisa fuscidentalis), cooked in soy sauce ("tsukudani"; oxya yezoensis, vespula flaviceps, and bombyx mori), dried (a. domesticus, l. migatoria, alphitobius diaperinus, tenebrio molitor, b. mori, hermetia illucens, and musca domestica), powdered (h. illucens, t. molitor) and other (incl. deep-frozen b. mori an ... | 2017 | 27903420 |
the culture of pediococcus pentosaceus t1 inhibits listeria proliferation in salmon fillets and controls maturation of kimchi. | the objective of this study is to evaluate the antilisterial effect of pediococcus pentosaceus t1, which was isolated from kimchi, and to assess its potential for extending the shelf life of salmon and kimchi. pediococcus pentosaceus t1 culture effectively inhibited proliferation of listeria monocytogenes in a dose-dependent manner in a salmon-based medium. antilisterial effect of the culture was stronger than that of nisin, an antibacterial peptide, as evidenced by lower minimum inhibitory conc ... | 2015 | 27904329 |
antagonistic effect of pseudomonas sp. cmi-1 on foodborne pathogenic listeria monocytogenes. | bacterial isolates derived from food or raw food materials of animal origin were screened for potential antagonistic activity against foodborne pathogenic listeria monocytogenes. using the agar spot method, ten out of the 94 tested bacteria showed antilisterial activity. all of the antagonistic isolates identified by sequence analysis as strains of the genus pseudomonas were able to inhibit the growth of all the examined listeria species including the ruminal pathogenic l. ivanovii and the oppor ... | 2015 | 27904352 |
the efficiency of uvc radiation in the inactivation of listeria monocytogenes on beef-agar food models. | the aim of this study is to evaluate the effect of meat content and surface smoothness on the deactivation of listeria monocytogenes in beef-agar food models achieved by shortwave ultraviolet (uvc) light. food models with various meat contents were made using chopped beef slices and agar solution. prepared models together with a listeria selective agar (lsa) plate and a slice of cooked beef were inoculated with l. monocytogenes and then exposed to uvc light. population of listeria reduced to bel ... | 2015 | 27904353 |
improved properties and microbiological safety of novel cottage cheese containing spices. | the study focuses on developing novel cottage cheese containing spices with acceptable sensory properties, increased biological value and extended shelf life. thirty types of cheese with added fresh or dried parsley, dill, pepper, garlic and rosemary were produced. characterisation of phenolic compounds, antioxidant capacity and antibacterial activity of spices and cheese samples were evaluated. the cheese containing fresh pepper and fresh and dried herbs showed excellent sensory properties, wit ... | 2015 | 27904380 |
microbiological quality and variability of natural microbiota in croatian cheese maturing in lambskin sacks. | as in the traditional production of cheese in lambskin sacks raw cow's or sheep's milk is mostly used, the purpose of this study is to see how the production affects the microbiological quality of the cheese. to do that, we tested 39 samples of raw cow's and sheep's milk, curd, ripened cheese (15, 30 and 45 days) and lambskin sacks for native microbial population. two-thirds of the milk, curd and cheese samples had higher counts of staphylococci and enterobacteria than permitted by regulations. ... | 2016 | 27904402 |
assay for listeria monocytogenes cells in whole blood using isotachophoresis and recombinase polymerase amplification. | we present a new approach which enables lysis, extraction, and detection of inactivated listeria monocytogenes cells from blood using isotachophoresis (itp) and recombinase polymerase amplification (rpa). we use an itp-compatible alkaline and proteinase k approach for rapid and effective lysis. we then perform itp purification to separate bacterial dna from whole blood contaminants using a microfluidic device that processes 25 μl sample volume. lysis, mixing, dispensing, and on-chip itp purifica ... | 2016 | 27904893 |
a dual filtration-based multiplex pcr method for simultaneous detection of viable escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus on fresh-cut cantaloupe. | fresh-cut cantaloupe is particularly susceptible to contamination with pathogenic bacteria, such as escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus. therefore, development of rapid, yet accurate detection techniques is necessary to ensure food safety. in this study, a multiplex pcr system and propidium monoazide (pma) concentration were optimized to detect all viable pathogens in a single tube. a dual filtration system utilized a filtration membrane with different por ... | 2016 | 27906992 |
chemical defence in a millipede: evaluation and characterization of antimicrobial activity of the defensive secretion from pachyiulus hungaricus (karsch, 1881) (diplopoda, julida, julidae). | the chemical defence of the millipede pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. in total, independently of sex, 44 compounds were identified. all compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. the relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. the two dominant quinones in b ... | 2016 | 27907048 |
the majority of genotypes of the virulence gene inla are intact among natural watershed isolates of listeria monocytogenes from the central california coast. | internalin a is an essential virulence gene involved in the uptake of the foodborne pathogen listeria monocytogenes into host cells. it is intact in clinical strains and often truncated due to premature stop codons (pmscs) in isolates from processed foods and processing facilities. less information is known about environmental isolates. we sequenced the inla alleles and did multi locus variable number tandem repeat analysis (mlva) on 112 l. monocytogenes isolates from a 3-year period from natura ... | 2016 | 27907153 |
experimental infection with listeria monocytogenes as a model for studying host interferon-γ responses. | l. monocytogenes is a gram-positive bacterium that is a cause of food borne disease in humans. experimental infection of mice with this pathogen has been highly informative on the role of innate and adaptive immune cells and specific cytokines in host immunity against intracellular pathogens. production of ifn-γ by innate cells during sublethal infection with l. monocytogenes is important for activating macrophages and early control of the pathogen(1-3). in addition, ifn-γ production by adaptive ... | 2016 | 27911410 |
subtilosin prevents biofilm formation by inhibiting bacterial quorum sensing. | subtilosin, the cyclic lantibiotic protein produced by bacillus subtilis katmira1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. in this study, subtilosin was purified using ammonium sulfate ((nh4)2so4) precipitation and purified via column chromatography. subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (mic and sub-mic) and anti-biofilm activity (biofilm prevention) were established. subtilosin was evaluated as a quorum ... | 2017 | 27914001 |
assessing vacuolar escape of listeria monocytogenes. | listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. in this chapter, we detail a microscopy fret-based assay which allows quantitatively measuring l. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morph ... | 2017 | 27914079 |
removal of listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments. | the effects of pronase (prn), cellulase (cel) or dnasei alone or combined with benzalkonium chloride (bac) against listeria monocytogenes-carrying biofilms were assayed. the best removal activity against l. monocytogenes-escherichia coli biofilms was obtained using dnasei followed by prn and cel. subsequently, a modified logistic model was used to quantify the combined effects of prn or dnasei with bac. a better bac performance after prn compared to dnasei eradicating l. monocytogenes was observ ... | 2017 | 27918204 |
tracking listeria monocytogenes contamination and virulence-associated characteristics in the ready-to-eat meat-based food products industry according to the hygiene level. | listeria monocytogenes isolates collected from final products and food contact surfaces of 10 ready-to-eat meat-based food products (rtemp) producing industries were analyzed to relate their virulence-associated characteristics and genetic profiles with the hygiene assessment of those industries. together with sample collection, an audit was performed to evaluate the implemented food safety management system and to investigate the specific audit requisites more associated to the occurrence of th ... | 2017 | 27919005 |
molecular identification and functional delineation of a glutathione reductase homolog from disk abalone (haliotis discus discus): insights as a potent player in host antioxidant defense. | glutathione reductase (gsr) is an enzyme that catalyzes the biochemical conversion of oxidized glutathione (gssg) into the reduced form (gsh). since the ratio between the two forms of glutathione (gsh/gssg) is important for the optimal function of gsh to act as an antioxidant against h2o2, the contribution of gsr as an enzymatic regulatory agent to maintain the proper ratio is essential. abalones are marine mollusks that frequently encounter environmental factors that can trigger the overproduct ... | 2017 | 27919756 |
effects of tween 80 on growth and biofilm formation in laboratory media. | tween 80 is a widely used non-ionic emulsifier that is added to cosmetics, pharmaceuticals, and foods. because of its widespread use we need to understand how it affects bacteria on our skin, in our gut, and in food products. the aim of this study is to investigate how tween 80 affects the growth and antimicrobial susceptibility of staphylococcus aureus, listeria monocytogenes, and pseudomonas fluorescens, which are common causes of spoilage and foodborne illnesses. addition of 0.1% tween 80 to ... | 2016 | 27920774 |
antibacterial, antioxidant and anti-proliferative properties and zinc content of five south portugal herbs. | crataegus monogyna l. (rosaceae) (cm), equisetum telmateia l. (equisataceae) (et), geranium purpureum vil. (geraniaceae) (gp), mentha suaveolens ehrh. (lamiaceae) (ms), and lavandula stoechas l. spp. luisieri (lamiaceae) (ls) are all medicinal. | 2017 | 27925492 |
innate immunity based cancer immunotherapy: b16-f10 murine melanoma model. | using killed microorganisms or their parts to stimulate immunity for cancer treatment dates back to the end of 19(th) century. since then, it undergone considerable development. our novel approach binds ligands to the tumor cell surface, which stimulates tumor phagocytosis. the therapeutic effect is further amplified by simultaneous application of agonists of toll-like receptors. we searched for ligands that induce both a strong therapeutic effect and are safe for humans. | 2016 | 27927165 |
brain abscess due to listeria monocytogenes after hellp syndrome in a patient with antiphospholipid syndrome. | to illustrate an unusual case of listeria cerebral abscess. | 2016 | 27927881 |
structural basis for glutathione-mediated activation of the virulence regulatory protein prfa in listeria. | infection by the human bacterial pathogen listeria monocytogenes is mainly controlled by the positive regulatory factor a (prfa), a member of the crp/fnr family of transcriptional activators. published data suggest that prfa requires the binding of a cofactor for full activity, and it was recently proposed that glutathione (gsh) could fulfill this function. here we report the crystal structures of prfa in complex with gsh and in complex with gsh and its cognate dna, the hly operator prfa box mot ... | 2016 | 27930316 |
licheniocin 50.2 and bacteriocins from lactococcus lactis subsp. lactis biovar. diacetylactis bgbu1-4 inhibit biofilms of coagulase negative staphylococci and listeria monocytogenes clinical isolates. | coagulase negative staphylococci (cons) and listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. the aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of cons and l. monocytogenes genital isolates. | 2016 | 27930711 |
draft genome sequences of two historical listeria monocytogenes strains from human listeriosis cases in 1933. | we report here the draft genome sequences of two listeria monocytogenes strains from some of the earliest reported cases of human listeriosis in north america. the strains were isolated in 1933 from patients in massachusetts and connecticut, usa, and belong to the widely disseminated hypervirulent clonal complex 1 (cc1) and cc2. | 2016 | 27932656 |
the role of stress and stress adaptations in determining the fate of the bacterial pathogen listeria monocytogenes in the food chain. | the foodborne pathogen listeria monocytogenes is a highly adaptable organism that can persist in a wide range of environmental and food-related niches. the consumption of contaminated ready-to-eat foods can cause infections, termed listeriosis, in vulnerable humans, particularly those with weakened immune systems. although these infections are comparatively rare they are associated with high mortality rates and therefore this pathogen has a significant impact on food safety. l. monocytogenes can ... | 2016 | 27933042 |
inflammation-induced cd69(+) kupffer cell feedback inhibits t cell proliferation via membrane-bound tgf-β1. | kupffer cells, tissue-resident macrophage lineage cell, are enriched in vertebrate liver. the mouse f4/80(+) kupffer cells have been subclassified into two subpopulations according to their phenotype and function: cd68(+) subpopulation with potent reactive oxygen species (ros) production and phagocytic capacities, and cd11b(+) subpopulation with a potent capacity to produce t helper 1 cytokines. in addition, cd11b(+) kupffer cells/macrophages may be migrated from the bone marrow or spleen, espec ... | 2016 | 27933593 |
microfluidic air sampler for highly efficient bacterial aerosol collection and identification. | the early warning capability of the presence of biological aerosol threats is an urgent demand in ensuing civilian and military safety. efficient and rapid air sample collection in relevant indoor or outdoor environment is a key step for subsequent analysis of airborne microorganisms. herein, we report a portable battery-powered sampler that is capable of highly efficient bioaerosol collection. the essential module of the sampler is a polydimethylsiloxane (pdms) microfluidic chip, which consiste ... | 2016 | 27934096 |
utilization of multiple substrates by butyrate kinase from listeria monocytogenes. | listeria monocytogenes, the causative agent of listeriosis, can build up to dangerous levels in refrigerated foods potentially leading to expensive product recalls. an important aspect of the bacterium's growth at low temperatures is its ability to increase the branched-chain fatty acid anteiso c15:0 content of its membrane at lower growth temperatures, which imparts greater membrane fluidity. mutants in the branched-chain α-keto dehydrogenase (bkd) complex are deficient in branched-chain fatty ... | 2017 | 27940001 |
modeling stochastic variability in the numbers of surviving salmonella enterica, enterohemorrhagic escherichia coli, and listeria monocytogenes cells at the single-cell level in a desiccated environment. | despite effective inactivation procedures, small numbers of bacterial cells may still remain in food samples. the risk that bacteria will survive these procedures has not been estimated precisely because deterministic models cannot be used to describe the uncertain behavior of bacterial populations. we used the poisson distribution as a representative probability distribution to estimate the variability in bacterial numbers during the inactivation process. strains of four serotypes of salmonella ... | 2017 | 27940547 |
in vitro evaluation of beneficial properties of bacteriocinogenic lactobacillus plantarum st8sh. | lactobacillus plantarum st8sh, isolated from bulgarian salami "shpek" and previously characterized as bacteriocin producer, was evaluated for its beneficial properties. based on the pcr analysis, lb. plantarum st8sh was shown to host a gene related to the production of adhesion proteins such as mab, mub, ef, and prgb. genetic and physiological tests suggest lb. plantarum st8sh to represent a potential probiotic candidate, including survival in the presence of low levels of ph and high levels of ... | 2016 | 27943050 |
microbial quality and suitability of roof-harvested rainwater in rural villages for crop irrigation and domestic use. | the study aimed at assessing the microbiological quality and suitability of roof-harvested rainwater (rhrw) for crop irrigation and domestic use. in total, 80 rainwater tanks (246 samples) across three rural villages (ga-molepane, jericho and luthngele) were visited. culture-based techniques were used to isolate bacterial microbes and identities were confirmed using matrix-assisted laser desorption/ionization time of flight (maldi-tof-ms). uncultured fungal populations were also identified using ... | 2016 | 27959874 |
polyethylene oxide (peo)-hyaluronic acid (ha) nanofibers with kanamycin inhibits the growth of listeria monocytogenes. | listeria monocytogenes is well known to cause prosthetic joint infections in immunocompromised patients. in this study, polyethylene oxide (peo) nanofibers, containing kanamycin and hyaluronic acid (ha), were prepared by electrospinning at a constant electric field of 10kv. peo nanofibers spun with 0.2% (w/v) ha and 1% (w/v) kanamycin had a smooth, bead-free structure at 30-35% relative humidity. the average diameter of the nanofibers was 83±20nm. attenuated total reflectance (atr)-fourier trans ... | 2017 | 27960136 |
the 12 gastrointestinal pathogens spectrum of acute infectious diarrhea in a sentinel hospital, shenzhen, china. | acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. the pathogen spectrum may differ among different regions and seasons. to investigate the etiology of acute diarrhea in shenzhen, a prospective study was conducted from august 2014 to september 2015. stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. the 19 pathogens were detected b ... | 2016 | 27965649 |
a bioengineered nisin derivative, m21a, in combination with food grade additives eradicates biofilms of listeria monocytogenes. | the burden of foodborne disease has large economic and social consequences worldwide. despite strict regulations, a number of pathogens persist within the food environment, which is greatly contributed to by a build-up of resistance mechanisms and also through the formation of biofilms. biofilms have been shown to be highly resistant to a number of antimicrobials and can be extremely difficult to remove once they are established. in parallel, the growing concern of consumers regarding the use of ... | 2016 | 27965658 |
biomarker tools to design clinical vaccines determined from a study of annual listeriosis incidence in northern spain. | two regions of northern spain, gipuzkoa, and cantabria present high annual incidence of listeriosis (1.86 and 1.71 cases per 100,000 inhabitants, respectively). we report that the high annual incidences are a consequence of infection with highly virulent listeria monocytogenes isolates linked to fatal outcomes in elderly patients with cancer. in addition, listeriosis patients with cancer present low il-17a/il-6 ratios and significantly reduced levels of anti-gapdh1-22 antibodies, identified as t ... | 2016 | 27965668 |
successful treatment of refractory listeria meningitis and bacteremia with trimethoprim-sulfamethoxazole in an immunocompetent child. | listeria monocytogenes is an important cause of life-threatening bacteremia and meningoencephalitis in neonates, pregnant women, the elderly, and immunocompromised individuals. however, it is an uncommon cause of illness in immunocompetent children beyond the neonatal period. ampicillin with or without an aminoglycoside remains the best treatment for listeriosis. here, we report a rare case of listeria meningitis and bacteremia in a 7-month-old immunocompetent girl, which was refractory to ampic ... | 2016 | 27976567 |
preparation and characterisation of chlorogenic acid-gelatin: a type of biologically active film for coating preservation. | chlorogenic acid (cga), a type of plant polyphenol, was conjugated onto gelatin (gel) to prepare a novel coating material for the preservation of fresh seafood. the optimal reaction molar ratio of cga to gelatin (4:1) was determined according to the cga content in the cga-gel conjugate. cga was confirmed to be successfully conjugated onto gelatin by (1)h nuclear magnetic resonance and fourier transform-infrared spectroscopy. the antioxidant activity of cga-gel was proven to be higher than that o ... | 2017 | 27979255 |
prevalence and antibiotic susceptibility profiles of listeria monocytogenes contamination of chicken flocks and meat in oyo state, south-western nigeria: public health implications. | food contamination with listeria monocytogenes is on the increase posing threats to public health with growing trends in food products recalls due to suspected listeria contamination. | 2016 | 27980380 |
involvement of il-17a-producing tcr γδ t cells in late protective immunity against pulmonary mycobacterium tuberculosis infection. | interleukin (il)-17a is a cytokine originally reported to induce neutrophil-mediated inflammation and anti-microbial activity. the cd4(+) t cells, which produce il-17a, have been well characterized as th17 cells. on the other hand, il-17a-producing tcr γδ(+) t cells have been reported to participate in the immune response at an early stage of infection with listeria monocytogenes and mycobacterium bovis in mice. however, the involvement of il-17a in protective immunity was not clearly demonstrat ... | 2016 | 27980775 |
production of bacterial ghosts from gram-positive pathogen listeria monocytogenes. | the gram-positive bacterium listeria monocytogenes is a ubiquitous intracellular pathogen, which has been implicated within the past decade as the causative organism in several outbreaks of foodborne disease. bacterial ghosts (bgs) are nonliving and vacant cell envelopes of bacteria generated by releasing the bacterial cytoplasm through a channel in the cell envelope. this study attempted to produce gram-positive pathogenic l. monocytogenes ghosts (lmgs) with simple chemicals. | 2017 | 27982711 |
novel cadmium resistance determinant in listeria monocytogenes. | listeria monocytogenes is a foodborne pathogen that can cause severe disease (listeriosis) in susceptible individuals. it is ubiquitous in the environment and often exhibits resistance to heavy metals. one of the determinants that enables listeria to tolerate exposure to cadmium is the cadac efflux system, with cada being a p-type atpase. three different cada genes (designated cada1 to cada3) were previously characterized in l. monocytogenes a novel putative cadmium resistance gene (cada4) was r ... | 2017 | 27986731 |
insight into the genome of brochothrix thermosphacta, a problematic meat spoilage bacterium. | brochothrix thermosphacta is a dominant but poorly studied meat spoilage organism. it is a close relative of the foodborne pathogen listeria monocytogenes, and brochothrix constitutes the second genus in the listeriaceae family. here, the genomes of 12 b. thermosphacta strains were sequenced, assembled into draft genomes, characterized, and compared with the genomes of brochothrix campestris and l. monocytogenes phenotypic properties including biogenic amine production and antibiotic and heavy m ... | 2017 | 27986732 |
the effect of preparation processes on the physicochemical characteristics and antibacterial activity of chitooligosaccharides. | the bioactivities of chitooligosaccharides are markedly influenced by the degree of acetylation, degree of polymerization or molecular weight and pattern of acetylation. thus, it is crucial to identify reproducible processes that will give rise to well-defined chitooligosaccharides and establish methods for their posterior physicochemical characterization in order to advance in the knowledge of their bioactivity. chitooligosaccharides were prepared by two different processes. the first used chit ... | 2017 | 27987925 |
purification of antilisterial peptide (subtilosin a) from novel bacillus tequilensis fr9 and demonstrate their pathogen invasion protection ability using human carcinoma cell line. | this study focuses on isolation, screening, and characterization of novel probiotics from gastrointestinal tract of free-range chicken (gallus gallus domesticus). fifty seven colonies were isolated and three isolates (fr4, fr9, and fr12) were selected and identified as lactobacillus gasseri fr4, bacillus tequilensis fr9, and l. animalis fr12 by 16s rrna sequencing. three strains were able to survive in stimulated acidic and bile conditions and inhibit the growth of pathogens. especially, fr9 exh ... | 2016 | 27990138 |
a systematic investigation of parameters influencing droplet rain in the listeria monocytogenes prfa assay - reduction of ambiguous results in ddpcr. | the droplet digital polymerase chain reaction (ddpcr) determines dna amounts based upon the pattern of positive and negative droplets, according to poisson distribution, without the use of external standards. however, division into positive and negative droplets is often not clear because a part of the droplets has intermediate fluorescence values, appearing as "rain" in the plot. despite the droplet rain, absolute quantification with ddpcr is possible, as shown previously for the prfa assay in ... | 2016 | 27992475 |
development and anti-listerial activity of pe-based biological preservative films incorporating plantaricin bm-1. | in recent years, bacteriocin as a natural antimicrobial compound, provides enormous promise to be used in food safety preservation. in this work, the polyethylene(pe)-based biological preservative films incorporating plantaricin bm-1, a typical iia bacteriocin produced by lactobacillus plantarum bm-1, were developed and characterized. the results showed that polyethylene (pe), low-density polyethylene (ldpe) and high-density polyethylene (hdpe) films soaked in plantaricin bm-1 solution had obvio ... | 2016 | 27993928 |
evaluation of hygienic quality and labelling of fish distributed in public canteens of northeast italy. | over the past few years, the demand for the introduction of fish products in public canteens (schools, hospitals and nursing-homes) has grown due to their good nutritional proprieties. the particular health conditions and sensitivity of some groups of consumers exposes them to greater risks of food poisoning. it is therefore important to monitor the raw materials that end up in mass catering implementing strategies of mass catering control, both with self-monitoring strategies and with regular c ... | 2016 | 27995098 |
benzalkonium tolerance genes and outcome in listeria monocytogenes meningitis. | listeria monocytogenes is a food-borne pathogen that can cause meningitis. the listerial genotype st6 has been linked to increasing rates of unfavourable outcome over time. we investigated listerial genetic variation and the relation with clinical outcome in meningitis. | 2017 | 27998823 |
comparative genomics analyses revealed two virulent listeria monocytogenes strains isolated from ready-to-eat food. | listeria monocytogenes is an important foodborne pathogen that causes considerable morbidity in humans with high mortality rates. in this study, we have sequenced the genomes and performed comparative genomics analyses on two strains, lm115 and lm41, isolated from ready-to-eat food in malaysia. | 2016 | 27999619 |
cell-based screen identifies human interferon-stimulated regulators of listeria monocytogenes infection. | the type i interferon (ifn) activated transcriptional response is a critical antiviral defense mechanism, yet its role in bacterial pathogenesis remains less well characterized. using an intracellular pathogen listeria monocytogenes (lm) as a model bacterial pathogen, we sought to identify the roles of individual interferon-stimulated genes (isgs) in context of bacterial infection. previously, ifn has been implicated in both restricting and promoting lm growth and immune stimulatory functions in ... | 2016 | 28002492 |
surface-enhanced raman spectroscopy introduced into the international standard organization (iso) regulations as an alternative method for detection and identification of pathogens in the food industry. | we show that surface-enhanced raman spectroscopy (sers) coupled with principal component analysis (pca) can serve as a fast, reliable, and easy method for detection and identification of food-borne bacteria, namely salmonella spp., listeria monocytogenes, and cronobacter spp., in different types of food matrices (salmon, eggs, powdered infant formula milk, mixed herbs, respectively). the main aim of this work was to introduce the sers technique into three iso (6579:2002; 11290-1:1996/a1:2004; 22 ... | 2017 | 28004171 |
a20 curtails primary but augments secondary cd8(+) t cell responses in intracellular bacterial infection. | the ubiquitin-modifying enzyme a20, an important negative feedback regulator of nf-κb, impairs the expansion of tumor-specific cd8(+) t cells but augments the proliferation of autoimmune cd4(+) t cells. to study the t cell-specific function of a20 in bacterial infection, we infected t cell-specific a20 knockout (cd4-cre a20(fl/fl)) and control mice with listeria monocytogenes. a20-deficient pathogen-specific cd8(+) t cells expanded stronger resulting in improved pathogen control at day 7 p.i. im ... | 2016 | 28004776 |
passive immunization with anti-acta and anti-listeriolysin o antibodies protects against listeria monocytogenes infection in mice. | listeria monocytogenes is an intracellular pathogen that causes listeriosis. due to its intracellular niche, l. monocytogenes has evolved to limit immune recognition and response to infection. antibodies that are slightly induced by listerial infection are completely unable to protect re-infection of l. monocytogenes. thus, a role of antibody on the protective effect against l. monocytogenes infection has been neglected for a long time. in the present study, we reported that passive immunization ... | 2016 | 28004800 |
evaluation of molecular typing of foodborne pathogens in european reference laboratories from 2012 to 2013. | in 2012, the european centre for disease prevention and control (ecdc) initiated external quality assessment (eqa) schemes for molecular typing including the national public health reference laboratories in europe. the overall aim for these eqa schemes was to enhance the european surveillance of food-borne pathogens by evaluating and improving the quality and comparability of molecular typing. the eqas were organised by statens serum institut (ssi) and included salmonella enterica subsp. enteric ... | 2016 | 28006653 |
thermal inactivation and sublethal injury kinetics of salmonella enterica and listeria monocytogenes in broth versus agar surface. | the objective of the present study was to compare the thermal inactivation and sublethal injury kinetics of salmonella enterica and listeria monocytogenes in broth (suspended cells) and on solid surface (agar-seeded cells). a 3-strain cocktail of s. enterica or l. monocytogenes inoculated in broth or on agar was subjected to heating in a water bath at various set temperatures (55.0, 57.5 and 60.0°c for s. enterica and 60.0, 62.5 and 65°c for l. monocytogenes). the occurrence of sublethally injur ... | 2017 | 28011300 |
sensitive detection of listeria monocytogenes based on highly efficient enrichment with vancomycin-conjugated brush-like magnetic nano-platforms. | pathogens pose a significant threat to public health worldwide. despite many technological advances in the rapid diagnosis of pathogens, sensitive pathogen detection remains challenging because target pathogenic bacteria usually exist in complex samples at very low concentrations. here, the construction of multivalent brush-like magnetic nanoprobes and their application for the efficient enriching of pathogens are demonstrated. brush-like magnetic nanoprobes were constructed by modification with ... | 2017 | 28013018 |
[spontaneous bacterial peritonitis with listeria monocytogenes leading to a cirrhosis discovery]. | 2016 | 28017475 | |
semi-industrial microwave treatments positively affect the quality of orange-colored smoothies. | thermal processing extends the shelf life of fruit and vegetables products by inactivating microorganisms and enzymes. the effect of a pasteurization (p) treatment, 90 ± 2 °c for 35 s, provided by continuous semi-industrial microwave (mw) under different conditions (high power/short time and low power/long time) or conventional pasteurization (cp) on orange-colored smoothies and their changes throughout 45 days of storage at 5 °c were investigated. results indicated that vitamin c and antioxidan ... | 2016 | 28017984 |
construction of antimicrobial peptide-drug combination networks from scientific literature based on a semi-automated curation workflow. | considerable research efforts are being invested in the development of novel antimicrobial therapies effective against the growing number of multi-drug resistant pathogens. notably, the combination of different agents is increasingly explored as means to exploit and improve individual agent actions while minimizing microorganism resistance. although there are several databases on antimicrobial agents, scientific literature is the primary source of information on experimental antimicrobial combin ... | 2016 | 28025336 |
application of a puffer fish skin gelatin film containing moringa oleifera lam. leaf extract to the packaging of gouda cheese. | this study aims to develop a puffer fish skin gelatin (psg) film that contains moringa oleifera lam. leaf extract (me) as a new biodegradable film. with the increase in me concentration, the tensile strength and elongation at break of the psg film increased, whereas the oxygen permeability and water vapor permeability decreased. in addition, the psg film with me exhibited antimicrobial activity against listeria monocytogenes and antioxidant activity. to apply the me-containing psg film to food p ... | 2016 | 28035143 |
a rapid typing method for listeria monocytogenes based on high-throughput multilocus sequence typing (hi-mlst). | listeria monocytogenes infects humans via food products, causing listeriosis. consequently, food companies pay meticulous attention to the risk of contamination of their products by this bacterium. while fragment analysis methods such as pulsed-field gel electrophoresis (pfge) are used to trace the sources of contamination for this bacterium, some drawbacks have been identified, namely the complexity of the methods and the difficulty of making data comparisons. as an alternative, multilocus sequ ... | 2017 | 28038334 |
an effective counterselection system for listeria monocytogenes and its use to characterize the monocin genomic region of strain 10403s. | construction of listeria monocytogenes mutants by allelic exchange has been laborious and time-consuming due to lack of proficient selection markers for the final recombination event, that is, a marker conveying substance sensitivity to the bacteria bearing it, enabling the exclusion of merodiploids and selection for plasmid loss. in order to address this issue, we engineered a counterselection marker based on a mutated phenylalanyl-trna synthetase gene (phes*). this mutation renders the phenyla ... | 2017 | 28039138 |
endoplasmic reticulum chaperone gp96 controls actomyosin dynamics and protects against pore-forming toxins. | during infection, plasma membrane (pm) blebs protect host cells against bacterial pore-forming toxins (pfts), but were also proposed to promote pathogen dissemination. however, the details and impact of blebbing regulation during infection remained unclear. here, we identify the endoplasmic reticulum chaperone gp96 as a novel regulator of pft-induced blebbing. gp96 interacts with non-muscle myosin heavy chain iia (nmhciia) and controls its activity and remodelling, which is required for appropri ... | 2017 | 28039206 |
application of a 222-nm krypton-chlorine excilamp to control foodborne pathogens on sliced cheese surfaces and characterization of the bactericidal mechanisms. | this study was conducted to investigate the basic spectral properties of a 222-nm krypton-chlorine (krcl) excilamp and its inactivation efficacy against major foodborne pathogens on solid media, as well as on sliced cheese compared to a conventional 254-nm low-pressure mercury (lp hg) lamp. selective media and sliced cheese inoculated with escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes were irradiated with a krcl excilamp and a lp hg lamp at the sam ... | 2017 | 28039742 |
inactivation and changes in metabolic profile of selected foodborne bacteria by 460 nm led illumination. | the objective of this study was to investigate the effect of 460 nm light-emitting diode (led) on the inactivation of foodborne bacteria. additionally, the change in the endogenous metabolic profile of led illuminated cells was analyzed to understand the bacterial response to the led illumination. six different species of bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, pseudomonas aeruginosa and salmonella typhimurium) were illuminated with 460 ... | 2017 | 28040158 |
occurrence and characterization of food-borne pathogens isolated from fruit, vegetables and sprouts retailed in the czech republic. | food of non-animal origin is a major component of the human diet and has been considered to pose a low risk from the point of view of bacteriological safety. however, an increase in the number of outbreaks of illness caused by such pathogens and linked to the consumption of fresh fruit and vegetables have been reported from around the world recently. salmonella spp., stec (shiga toxin producing escherichia coli) and listeria monocytogenes are among the most frequently identified agents. addition ... | 2017 | 28040162 |
differential gene expression and filamentation of listeria monocytogenes 08-5923 exposed to sodium lactate and sodium diacetate. | this study reports the gene expression and filamentation in listeria monocytogenes 08-5923 following exposure to food preservatives sodium lactate (nal) and sodium diacetate (sd). l. monocytogenes 08-5923 was challenged with a mixture of nal/sd, nal or sodium acetate at 37 °c in tryptic soy broth. in the initial study, l. monocytogenes 08-5923 was exposed to nal/sd for 24 h. the transcriptome was investigated by rna sequencing. a stress response network was discovered in l. monocytogenes 08-5923 ... | 2017 | 28040163 |
bacteriocin-like substances of lactobacillus curvatus p99: characterization and application in biodegradable films for control of listeria monocytogenes in cheese. | the aim of this study was to evaluate the effectiveness of a biodegradable film, with antimicrobial metabolites produced by lactobacillus curvatus p99 incorporated, targeting the control of listeria monocytogenes in sliced "prato" cheese. tests were performed to evaluate the spectrum of action of cell-free supernatant (cfs) of p99 against different microorganisms, as well as to detect the minimum inhibitory (mic) and bactericidal (mbc) concentrations against l. monocytogenes scott a. the detecti ... | 2017 | 28040164 |
application of low frequency pulsed ohmic heating for inactivation of foodborne pathogens and ms-2 phage in buffered peptone water and tomato juice. | the purpose of this study was to inactivate foodborne pathogens effectively by ohmic heating in buffered peptone water and tomato juice without causing electrode corrosion and quality degradation. escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes were used as representative foodborne pathogens and ms-2 phage was used as a norovirus surrogate. buffered peptone water and tomato juice inoculated with pathogens were treated with pulsed ohmic heating at different frequencie ... | 2017 | 28040173 |
detection of premature stop codons leading to truncated internalin a among food and clinical strains of listeria monocytogenes. | listeria monocytogenes is a food-borne pathogen responsible for outbreaks and sporadic cases of listeriosis, a severe invasive disease. internalin a (inla) a protein encoded by inla has a key role in the mechanism of pathogenesis in l. monocytogenes infection, specifically in the invasion of human intestinal epithelial cells. studies on inla have shown that mutations leading to premature stop codons (pmscs) occur naturally and are associated with impaired virulence of l. monocytogenes strains. i ... | 2017 | 28040183 |
inhibition of crispr-cas9 with bacteriophage proteins. | bacterial crispr-cas systems utilize sequence-specific rna-guided nucleases to defend against bacteriophage infection. as a countermeasure, numerous phages are known that produce proteins to block the function of class 1 crispr-cas systems. however, currently no proteins are known to inhibit the widely used class 2 crispr-cas9 system. to find these inhibitors, we searched cas9-containing bacterial genomes for the co-existence of a crispr spacer and its target, a potential indicator for crispr in ... | 2017 | 28041849 |
structural characterisation of the fatty acid biosynthesis enzyme fabf from the pathogen listeria monocytogenes. | development of new antimicrobial agents is required against the causative agent for listeriosis, listeria monocytogenes, as the number of drug resistant strains continues to increase. a promising target is the β-ketoacyl-acyl carrier protein synthase fabf, which participates in the catalysis of fatty acid synthesis and elongation, and is required for the production of phospholipid membranes, lipoproteins, and lipopolysaccharides. in this study, we report the 1.35 å crystal structure of fabf from ... | 2017 | 28045020 |
cord blood acute phase reactants predict early onset neonatal sepsis in preterm infants. | early onset sepsis (eos) is a major cause of morbidity and mortality in preterm infants, yet diagnosis remains inadequate resulting in missed cases or prolonged empiric antibiotics with adverse consequences. evaluation of acute phase reactant (apr) biomarkers in umbilical cord blood at birth may improve eos detection in preterm infants with intrauterine infection. | 2017 | 28045978 |
presence of foodborne pathogens, extended-spectrum β-lactamase -producing enterobacteriaceae, and methicillin-resistant staphylococcus aureus in slaughtered reindeer in northern finland and norway. | various food-producing animals were recognized in recent years as healthy carriers of bacterial pathogens causing human illness. in northern fennoscandia, the husbandry of semi-domesticated reindeer (rangifer tarandus tarandus) is a traditional livelihood and meat is the main product. this study determined the presence of selected foodborne pathogens, methicillin-resistant staphylococcus aureus (mrsa), and extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in healthy semi-domestic ... | 2017 | 28049493 |
singleton sequence type 382, an emerging clonal group of listeria monocytogenes associated with three multistate outbreaks linked to contaminated stone fruit, caramel apples, and leafy green salad. | three multistate outbreaks between 2014 and 2016, involving case patients in and outside the united states, were linked to stone fruit, caramel apples, and packaged leafy green salad contaminated with listeria monocytogenes singleton sequence type 382 (st382), a serotype ivb-v1 clone with limited genomic divergence. isolates from these outbreaks and other st382 isolates not associated with these outbreaks were analyzed by whole-genome sequencing (wgs) analysis. the primary differences among st38 ... | 2017 | 28053218 |
microbiological and chemico-physical shelf-life and panel test to evaluate acceptability of liver mortadella. | this study aimed to evaluate the shelf life of sliced cooked liver mortadella packaged in map (70-85% n2, 15-30% co2) and stored in refrigeration (4°c) or slight thermal abuse (8°c) for up to 49 days (declared best before date 45 days). the proximate composition, aw nitrites and nacl content were determined at t0. weekly, samples were submitted to microbiological [total viable count (tvc), lactic acid bacteria (lab), enterobacteriaceae, escherichia coli, pseudomonas spp., coagulase positive stap ... | 2016 | 28058249 |
evaluation of the antibacterial activity of bergamot essential oils on different listeria monocytogenes strains. | essential oils are aromatic and volatile substances extracted from plants and characterized by antimicrobial activity. the aim of the present study was to evaluate the antibacterial activity (agar disc-diffusion method) of seven different bergamot essential oils (beos) on eight listeria monocytogenes strains. minimal inhibitory concentration (mic) of most efficient beos was estimated. extremely variable results for agar disc-diffusion method for l. monocytogenes strains were reported. one of the ... | 2016 | 28058251 |
risk assessment in the recovery of food for social solidarity purposes: preliminary data. | the most recent study, conducted by politecnico of milan, on food surplus management in italy shows that in the italian food supply chain the food surplus is around 5.5 million tons/year, and the amount of food wasted is around 5.1 million tons/year. during 2015, the charitable organizations (cos) belonging to the italian food bank network, active in recovering and distributing food for social solidarity's purposes, reused 381,345 tons of food from 2292 donors. the main supplying sources of the ... | 2016 | 28058252 |
evaluation of the 3m™ molecular detection assay (mda) 2 - listeria monocytogenes for the detection of listeria monocytogenes in a variety of foods and select environmental surfaces: collaborative study, first action 2016.08. | the 3m™ molecular detection assay (mda) 2 - listeria monocytogenes uses loop-mediated isothermal amplification of unique dna target sequences combined with bioluminescence to rapidly detect listeria monocytogenes in a broad range of food types and on environmental surfaces. using an unpaired study design, technicians from 13 laboratories located in the united states and canada compared the 3m mda 2 - listeria monocytogenes to the u.s. department of agriculture food safety inspection service micr ... | 2017 | 28059049 |
antibacterial and antivirulence effect of 6-n-hydroxylaminopurine in listeria monocytogenes. | the emerging development of antibiotic resistant bacteria calls for novel types of antibacterial agents. in this work we examined the putative antibacterial effect of purine analogs in listeria monocytogenes we show that, among several tested purine analogs, only 6-n-hydroxylaminopurine (6-n-hap) reduces the viability of the gram-positive pathogen listeria monocytogenes as in bacillus subtilis, 6-n-hap terminates expression at guanine riboswitches in l. monocytogenes hence preventing expression ... | 2017 | 28062853 |
multistate outbreak of listeria monocytogenes infections linked to whole apples used in commercially produced, prepackaged caramel apples: united states, 2014-2015. | whole apples have not been previously implicated in outbreaks of foodborne bacterial illness. we investigated a nationwide listeriosis outbreak associated with caramel apples. we defined an outbreak-associated case as an infection with one or both of two outbreak strains of listeria monocytogenes highly related by whole-genome multilocus sequence typing (wgmlst) from 1 october 2014 to 1 february 2015. single-interviewer open-ended interviews identified the source. outbreak-associated cases were ... | 2017 | 28065170 |
hospital-acquired listeriosis linked to a persistently contaminated milkshake machine. | one case of hospital-acquired listeriosis was linked to milkshakes produced in a commercial-grade shake freezer machine. this machine was found to be contaminated with a strain of listeria monocytogenes epidemiologically and molecularly linked to a contaminated pasteurized, dairy-based ice cream product at the same hospital a year earlier, despite repeated cleaning and sanitizing. healthcare facilities should be aware of the potential for prolonged listeria contamination of food service equipmen ... | 2017 | 28065212 |
bacterial meningitis in alcoholic patients: a population-based prospective study. | to study clinical features and outcome of community-acquired bacterial meningitis in alcoholic patients. | 2017 | 28065808 |
comparative genomics of the listeria monocytogenes st204 subgroup. | the st204 subgroup of listeria monocytogenes is among the most frequently isolated in australia from a range of environmental niches. in this study we provide a comparative genomics analysis of food and food environment isolates from geographically diverse sources. analysis of the st204 genomes showed a highly conserved core genome with the majority of variation seen in mobile genetic elements such as plasmids, transposons and phage insertions. most strains (13/15) harbored plasmids, which altho ... | 2016 | 28066377 |
inactivation of salmonella spp. and listeria spp. by palmitic, stearic, and oleic acid sophorolipids and thiamine dilauryl sulfate. | food contaminated with human pathogens, such as salmonella spp. and listeria monocytogenes, frequently causes outbreaks of foodborne illness. consumer concern over the use of synthesized antimicrobials to enhance microbial food safety has led to a search of natural alternatives. the objectives of this study were to evaluate the antimicrobial activity of various types of sophorolipids (sls) and thiamine dilauryl sulfate (tds) against pathogenic salmonella spp. and listeria spp. both free and lact ... | 2016 | 28066390 |
comparative genomic analysis of two serotype 1/2b listeria monocytogenes isolates from analogous environmental niches demonstrates the influence of hypervariable hotspots in defining pathogenesis. | the vast majority of clinical human listeriosis cases are caused by serotype 1/2a, 1/2b, 1/2c, and 4b isolates of listeria monocytogenes. the ability of l. monocytogenes to establish a systemic listeriosis infection within a host organism relies on a combination of genes that are involved in cell recognition, internalization, evasion of host defenses, and in vitro survival and growth. recently, whole genome sequencing and comparative genomic analysis have proven to be powerful tools for the iden ... | 2016 | 28066772 |
listeria monocytogenes circulating in rabbit meat products and slaughterhouses in italy: prevalence data and comparison among typing results. | rabbit meat has outstanding dietetic and nutritional properties. however, few data on microbiological hazards associated with rabbit productions are available. in this study, the presence of listeria monocytogenes was determined in 430 rabbit carcasses, 256 rabbit meat cuts and products, and 599 environmental sponges collected from four italian rabbit slaughterhouses over a period of 1 year. prevalence of l. monocytogenes among the 1285 rabbit meat and environmental samples was 11%, with statist ... | 2017 | 28067541 |
chosen quality parameters of pork sausage produced without curing mixture. | the aim of this study was to determine the influence of the dried celeriac juice addition, as a nitrogenous compounds sources, on the quality of the experimental pork sausage. | 2017 | 28068026 |
listeria monocytogenes behaviour and quality attributes during sausage storage affected by sodium nitrite, sodium lactate and thyme essential oil. | the effects of the addition of nitrite at 200 ppm (n), sodium lactate 1.5% (l) and thyme essential oil at 100 ppm (t1) on listeria monocytogenes behaviour and atpase activity inhibition were evaluated, as well as lipid oxidation through the quantification of malonaldehydes, in sausage stored at 8 ℃ for 41 days and at 30 ℃ for 14 days. the changes in the colour profile were performed during storage time at 8 ℃. quantitative descriptive sensory analyses were performed after two days at 4 ℃. at 8 ℃ ... | 2017 | 28068841 |
essential oils against pathogen and spoilage microorganisms of fruit juices: use of versatile antimicrobial delivery systems. | essential oils (eo) are increasingly used as natural antimicrobial compounds, however the effect of delivery system to enhance their antimicrobial activity has not been widely studied. limonene (0 to 10 μl/ml) was added to microbial suspensions (∼10(5) cfu/ml) of selected foodborne pathogens (listeria monocytogenes scott a, salmonella enterica typhimurium, escherichia coli and staphylococcus aureus), and spoilage microorganisms (lactobacillus plantarum, saccharomyces cerevisiae, and candida albi ... | 2017 | 28071802 |