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chlamydiaceae in riverine buffalo (bubalus bubalis) and cows (bos taurus) in egypt with and without signs of reproductive disease.to obtain information and compare the prevalence of chlamydiaceae in riverine buffalo (bubalus bubalis) and cows (bos taurus) in egypt with and without clinical signs of reproductive disease.201222712774
isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (bubalus bubalis) in argentina.parainfluenza virus type 3 (piv3) was isolated from dairy buffaloes (bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions.201222716217
molecular phylogeny of pakistani riverine buffalo based on genetic variability of mitochondrial cytochrome b gene.mitochondrial cytochrome b gene is considered to be one of the best markers for breed characterization as well as studying the ancestry in the vertebrates due to its exclusive maternal inheritance. dna fingerprinting by single nucleotide polymorphism is most reliable and widely used molecular technique in modern forensics and is being considered in this study. partial sequencing of 1,061 bp of aforementioned gene from 14580 to 15643 was conducted in two famous pakistani buffalo breeds named nili ...201222718511
nutritional evaluation of organically grown fodders in lactating murrah buffaloes (bubalus bubalis).a study was undertaken to evaluate the nutritional response of organically grown diets in buffaloes on nutrients utilization and nutrient efficiency for milk production. for this, ten milch murrah buffaloes of average body weight (490.72 ± 6.65 kg), milk yield (8.13 ± 0.33 kg), and lactation stage (85 ± 5.28 days) were distributed in inorganic (indg()) and organic dietary groups (ordg) with five animals in each. buffaloes of indg and ogdg were fed sorghum hay-berseem fodder-concentrate mixture-b ...201322733348
isolation and enrichment of type a spermatogonia from pre-pubertal buffalo ( bubalus bubalis) testis.the aim of this study was to isolate and subsequently enrich type a spermatogonia from pre-pubertal buffalo testis. two-step enzymatic digestion was used to isolate spermatogonia from 10 to 14 months pre-pubertal buffalo calves, resulting in maximal release of spermatogonia from the seminiferous tubules. after enzymatic digestion, the type a spermatogonia were subsequently enriched by differential plating and percoll gradient centrifugation. the identity of type a spermatogonia was determined by ...201322742713
collagen-iv supported embryoid bodies formation and differentiation from buffalo (bubalus bubalis) embryonic stem cells.embryoid bodies (ebs) are used as in vitro model to study early extraembryonic tissue formation and differentiation. in this study, a novel method using three dimensional extracellular matrices for in vitro generation of ebs from buffalo embryonic stem (es) cells and its differentiation potential by teratoma formation was successfully established. in vitro derived inner cell masses (icms) of hatched buffalo blastocyst were cultured on buffalo fetal fibroblast feeder layer for primary cell colony ...201222749767
prognosis prediction of uterine torsion mechanical treatment (rolling) after estimation of calcium and creatinine level in the serum of buffaloes (bubalus bubalis).the present study was carried out to investigate the relationship between creatinine and calcium concentration in buffalo serum in cases of uterine torsion before rolling, and 1 h and 24 h after calving. the degree, duration and site of uterine torsion, as well as fetus viability, time needed for cervical dilation, and the occurrence of uterine rupture were recorded. a total of 150 pregnant buffaloes suffering from colic and anorexia were brought to our clinic and clinically examined. of these, ...201222763076
animal reservoirs of visceral leishmaniasis in india.visceral leishmaniasis (vl) is a disease that has both zoonotic and anthroponotic etiologies. in india, vl is endemic, considered to be anthroponotic, and caused by leishmania donovani . anthroponotic diseases are maintained by transmission from human to human and to a lesser extent from human to animals. serum samples from 1,220 animals from 7 human vl endemic districts of bihar, india, were tested for antibodies to a recombinant kinetoplast antigen (rk39 antigen) present in amastigotes of visc ...201322765517
tissue-specific temporal exome capture revealed muscle-specific genes and snps in indian buffalo (bubalus bubalis).whole genome sequencing of buffalo is yet to be completed, and in the near future it may not be possible to identify an exome (coding region of genome) through bioinformatics for designing probes to capture it. in the present study, we employed in solution hybridization to sequence tissue specific temporal exomes (tst exome) in buffalo. we utilized cdna prepared from buffalo muscle tissue as a probe to capture tst exomes from the buffalo genome. this resulted in a prominent reduction of repeat s ...201222768984
molecular cloning, sequence characterization and heterologous expression of buffalo (bubalus bubalis) oviduct-specific glycoprotein in e. coli.oviductin is a high molecular weight oviduct-specific glycoprotein secreted by the non-ciliated epithelial cells of oviduct during estrous cycle and early pregnancy. it plays an important role during fertilization and early embryonic development. the oviductin gene from oviductal tissues of buffalo was successfully cloned and sequenced. the sequence analysis revealed that buffalo and cattle oviductin share very high homology between their cdna sequences. the predicted amino acid sequences of the ...201222782592
serum acute phase proteins in control and theileria annulata infected water buffaloes (bubalus bubalis).this study was carried out to ascertain the changes in acute phase proteins (apps) and pro-inflammatory cytokines in theileria annulata infected water buffalo. thirty infected water buffaloes and 20 parasitologically free were used. in the present study there was significant (p ≤ 0.05) increase in haptoglobin (hp), serum amyloid a (saa), ceruloplasmin, α1-acid glycoprotein (agp) and fibrinogen levels (2.18 ± 0.29 g/l, 156.58 ± 3.48 mg/l, 31.23 ± 1.25mg/dl, 370.23 ± 33.21 mg/l and 16.17 ± 1.18 g/ ...201222785130
buffalo (bubalus bubalis) es cell-like cells are capable of in vitro skeletal myogenic differentiation.when buffalo embryonic stem (es) cell-like cells that expressed surface markers ssea-4, tra-1-60, tra-1-81, cd9 and cd90 and intracellular markers oct4, sox2 and foxd3, as shown by immunofluorescence, and that expressed rex-1 and nucleostemin as confirmed by rt-pcr, were subjected to suspension culture in hanging drops in absence of lif and buffalo foetal fibroblast feeder layer support, they differentiated to form three-dimensional embryoid bodies (ebs). of 231 ebs examined on day 3 of suspensi ...201322788718
establishment and characterization of a buffalo (bubalus bubalis) mammary epithelial cell line.the objective of this study was to establish the buffalo mammary epithelial cell line (bumec) and characterize its mammary specific functions.201222792341
a nonsense mutation in the tyrosinase gene causes albinism in water buffalo.oculocutaneous albinism (oca) is an autosomal recessive hereditary pigmentation disorder affecting humans and several other animal species. oculocutaneous albinism was studied in a herd of murrah buffalo to determine the clinical presentation and genetic basis of albinism in this species.201222817390
effect of parity, days in milk, and milk yield on detailed milk protein composition in mediterranean water buffalo.the effects of some nongenetic factors on milk protein fraction contents and relative proportions were estimated in 606 individual milk samples of mediterranean water buffalo. content of α(s1)-casein (cn), α(s2)-cn, β-cn, γ-cn, κκ-cn, glycosylated κ-cn (glyco-κ-cn), α-lactalbumin, and β-lactoglobulin was measured by reversed-phase hplc. relative contents of α(s1)-cn%, α(s2)-cn%, β-cn%, and κ-cn% were, respectively, 32.1, 17.1, 34.5, and 15.7%, whereas γ-cn% accounted for 0.6% of total casein con ...201222818435
culture, characterization and differentiation of cells from buffalo (bubalus bubalis) amnion.stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. we report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, oct-4, nanog and sox-2) from the amnion of water buffalo (bubalus bubalis). the cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. the cytochemical staining revealed that pluripotent ...201322820992
molecular differentiation of peroxysome proliferator activated receptor coactivator-1 among different breeds of bubalus bubalis.peroxysome proliferator activated receptor coactivator-1 gene (ppargc1a) is a positional and functional candidate gene for milk fat yield. it has key role in energy, fat and glucose metabolism. single nucleotide polymorphisms (snps) in exon-8 of ppargc1a are reported to be associated with milk fat yield in dairy cattle. in the present investigation ppargc1a was partially amplified (around 767bp) by designing gene specific primer and confirm by sequencing the amplicon and its comparison with the ...201222829739
metagenomic analysis of virulence-associated and antibiotic resistance genes of microbes in rumen of indian buffalo (bubalus bubalis).a major research goal in rumen microbial ecology is to understand the relationship between community composition and its function, particularly involved in fermentation process is of a potential interest. the buffalo rumen microbiota impacts human food safety as well as animal health. although the bacteria of bovine rumen have been well characterized, techniques have been lacking to correlate total community structure with gene function. we applied 454 next generations sequencing technology to c ...201222850272
parentage verification in field progeny testing program of mehsana buffalo.the present study was undertaken to construct a multiplex microsatellite panel for parentage testing in mehsana buffalo (bubalus bubalis). the study was based on a total of 212 mehsana buffalos (100 dams, 100 daughters, and 12 sires). genomic dna was extracted from blood and semen samples. a panel of 10 microsatellite markers (cssm61, ilsts29, ilsts17, ilsts28, cssm57, cssm22, ilsts61, cssm8, eth152, and ilsts11) was amplified in a single multiplex reaction and analyzed by capillary electrophore ...201222851245
[polymorphisms of inhibin α gene exon 1 in buffalo (bubalus bubalis), gayal (bos frontalis) and yak (bos grunniens)].to elucidate the genetic characteristics of the bovine inhibin α subunit (inha) gene, the polymorphisms in exon 1 of inha and its bilateral sequences were assayed using pcr with direct sequencing in buffalo, gayal and yak. a comparative analysis was conducted by pooled the results in this study with the published data of inha on some mammals including some bovine species together. a synonymous substitution c.73c>a was identified in exon 1 of inha for buffalo, which results in identical encoding ...201222855448
immunolocalization, gene expression, and enzymatic activity of cyclooxygenases, prostaglandin e2-9-ketoreductase, and nitric oxide synthases in mediterranean buffalo (bubalus bubalis) corpora lutea during diestrus.immunopresence, gene expression, and enzymatic activity of cyclooxygenase 1 (cox1), cox2, pge2-9-ketoreductase (pge2-9-k), endothelial (enos), and inducible nitric oxide synthases (inos), and hormone in vitro production were examined in early, mid, late, and regressive buffalo corpora lutea (cl). cox1 immunosignals were detected in the cytoplasm of small luteal cells, cox2 in large luteal cells, and pge2-9-k in all luteal cells. cox2 and pge2-9-k immunosignals were greater in late cl. immunopres ...201222865504
cloning and sequencing of the rdna gene family of the water buffalo (bubalus bubalis).the rdna genes coding for ribosomal rna in animals are complicated repeat sequences with high gc content. we amplified water buffalo rdna gene sequences with the long and accurate (la) pcr method, using la taq dna polymerase and gc buffer, based on bioinformatic analysis of related organisms. the rdna genes were found to consist of 9016 nucleotides, including three rrna genes and two internal transcribed spacers (its), which we named 18s rrna, its1, 5.8s rrna, its2 and 28s rrna. we tested and op ...201222869068
interferon stimulated gene 15 (isg15): molecular characterization and expression profile in endometrium of buffalo (bubalus bubalis).interferon stimulated gene 15 (isg15), one of the several proteins induced by conceptus derived type i and/or a type ii interferon (ifn), is implicated as an important factor in determining the uterine receptivity and conceptus development. however, presence as well as specific role of the isg15 in buffalo (bubalus bubalis) reproduction is yet to be elucidated. in the present study, both genomic and cdna sequences of bubaline (bu) isg15 were cloned and investigated for its expression in differen ...201222871329
detailed description of rba-banded chromosomes of river buffalo (bubalus bubalis l.). 198422879164
male-specific repeats in wild bovidae.in this study, we investigated repetitive sequences localized on y chromosomes. repetitive dna sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for y chromosome separation, followed by polymerase chain reaction (pcr), cloning, and sequence analysis. we applied the derived repeat units to members of nine tri ...201222895838
the c-banding pattern of the egyptian water buffalo (bubalus bubalis). 197822896102
optimization of cryopreservation of buffalo (bubalus bubalis) blastocysts produced by in vitro fertilization and somatic cell nuclear transfer.the objective of this study was to optimize cryopreservation conditions for buffalo in vitro produced (ivp) embryos. the in vitro fertilized (ivf) and somatic cell nuclear transferred (scnt) blastocysts were vitrified with either 40% ethylene glycol (eg), 25% eg + 25% dimethylsulfoxide (dmso), or 20% eg + 20% dmso + 0.5 m sucrose, and the ivf blastocysts produced from abattoir-derived ovaries were also slow-frozen with either 10% eg or 0.05 m trehalose dehydrate + 1.8% eg + 0.4% bsa. cryosurviva ...201222925650
"remake" by high-throughput sequencing of the microbiota involved in the production of water buffalo mozzarella cheese.intermediates of production of two batches of traditional mozzarella cheese were analyzed by culture-independent pyrosequencing. the quantitative distribution of taxa within the samples suggested that thermophilic lactic acid bacteria from the natural starter were mainly responsible for the fermentation, while microorganisms found in raw milk did not develop during fermentation.201222941080
birth of cloned calves from vitrified-warmed zona-free buffalo (bubalus bubalis) embryos produced by hand-made cloning.the availability of techniques for the vitrification of cloned blastocysts can improve their effective use. the present study compared the developmental competence of buffalo cloned embryos derived from adult (baf), newborn (bnf) and fetal fibroblast (bff) before and after vitrification. despite similar cleavage rates among the three groups, the blastocyst rate was lower for baf- than bnf- and bff-derived embryos (30.2±2.2% vs 41.7±1.7% and 39.1±2.1%, respectively; p<0.01). the total cell number ...201322950886
rock inhibitor y-27632 enhances the survivability of dissociated buffalo (bubalus bubalis) embryonic stem cell-like cells.this study investigated the effects of supplementation of culture medium with 10 μm y-27632, a specific inhibitor of rho kinase activity, for 6 days on self-renewal of buffalo embryonic stem (es) cell-like cells at passage 50-80. y-27632 increased mean colony area (p<0.05) although it did not improve their survival. it decreased oct4 expression (p<0.05), increased nanog expression (p<0.05), but had no effect on sox2 expression. it also increased expression of anti-apoptotic gene bcl-2 (p<0.05) a ...201322951036
utilization of elisa using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of schistosoma japonicum infection among water buffaloes.the presence of animal reservoirs in schistosoma japonicum infection has been a major obstacle in the control of schistosomiasis. previous studies have proven that the inclusion of control measures on animal reservoir hosts for schistosomiasis contributed to the decrease of human cases. animal surveillance should therefore be included to strengthen and improve the capabilities of current serological tests.201222953018
expression of leptin and its receptor in corpus luteum during estrous cycle in buffalo (bubalus bubalis).leptin is supposed to play a crucial role in ovarian luteal dynamics. the present study was aimed to investigate the importance of leptin and its receptors in buffalo corpus luteum (cl) obtained from different stages of the estrous cycle. real-time rt-pcr (qpcr), western blot and immunohistochemistry techniques were applied to investigate mrna expression, protein expression and localization of examined factors. additionally to assess the contribution of leptin in progesterone production the expr ...201222959515
short communication: csn1s1-csn3 (α(s1)-κ-casein) composite genotypes affect detailed milk protein composition of mediterranean water buffalo.the aim of the study was to investigate the effect of composite csn1s1-csn3 [α(s1)-κ-casein (cn)] genotype on milk protein composition in mediterranean water buffalo. content of α(s1)-cn, α(s2)-cn, β-cn, γ-cn, κ-cn, glycosylated and unglycosylated κ-cn, α-lactalbumin, and β-lactoglobulin was measured by reversed-phase hplc using 621 individual milk samples. genotypes at csn1s1 and csn3 were also obtained by reversed-phase hplc. two alleles were detected at csn1s1 (corresponding to the a and b va ...201222959943
identity and public health potential of cryptosporidium spp. in water buffalo calves in egypt.little is known about the diversity and public health significance of cryptosporidium species in water buffaloes. in this study, we examined the distribution of cryptosporidium spp. in water buffalo calves in egypt. rectal fecal specimens from 179 calves and 359 adults were screened microscopically for cryptosporidium oocysts using modified ziehl-neelsen stain. cryptosporidium spp. in 17 microscopy-positive specimens from calves were genotyped by dna sequence analysis of the small-subunit rrna g ...201322963712
influence of season on corpus luteum structure and function and ai outcome in the italian mediterranean buffalo (bubalus bubalis).the aim was to ascertain whether relationships between corpus luteum (cl) vascularization, cl function, and pregnancy outcome in ai in buffaloes were consistent across the breeding season and transition period to the nonbreeding season in a mediterranean environment. stage of the estrous cycle in italian mediterranean buffaloes was synchronized using the ovsynch with timed ai program and buffaloes were mated by ai in both the breeding season (n = 131) and transition period (n = 125). detailed in ...201222979961
a new translocation t(1p;18) in an italian mediterranean river buffalo (bubalus bubalis, 2n = 50) bull: cytogenetic, fertility and inheritance studies.in recent years increasing attention has been paid to the cytogenetic control of italian mediterranean river buffalo (bbu) bulls authorized as sires which are registered in the stud book. chromosome abnormalities described in this species are mainly numerical and affecting sex chromosomes. during routine cytogenetic analyses performed on young italian mediterranean river buffalo bulls in the progeny test, 1 animal was found to be carrier of a never before reported translocation t(1p;18) originat ...201322986410
the correlation between age, body weight and testicular parameters in murrah buffalo bulls raised in brazil.buffalo are an economically important source for meat and milk production, especially in brazil. however, important aspects of their biology remain unknown thus far. herein, we describe the reproductive characteristics of male murrah buffalo (bubalus bubalis) raised under extensive management conditions by applying biometrics associated with testicular weight. we analyzed seven males, divided into two groups: g1, which consisted of four 18-month-old animals, and g2, which consisted of three 24-m ...201222986925
sex-sorting sperm using flow cytometry/cell sorting.the sex of mammalian offspring can be predetermined by flow sorting relatively pure living populations of x- and y-chromosome-bearing sperm. this method is based on precise staining of the dna of sperm with the nucleic acid-specific fluorophore, hoechst 33342, to differentiate between the subpopulations of x- and y-sperm. the fluorescently stained sperm are then sex-sorted using a specialized high speed sorter, moflo(®) sx xdp, and collected into biologically supportive media prior to reconcentr ...201322992923
anatomic reference for computed tomography of paranasal sinuses and their communication in the egyptian buffalo (bubalus bubalis).the purpose of this work was to present an anatomic reference for computed tomography (ct) for the paranasal sinuses of adult buffalo fit the use of anatomists, radiologists, clinicians and veterinary students. ct images with the most closely corresponding cross sections of the head were selected and studied serially in a rostral to caudal progression from the level of the interdental space to the level of the nuchal line. the anatomical features were compared with the dissected heads and skulls ...201322994483
a single nucleotide polymorphism in the promoter region of river buffalo stearoyl coa desaturase gene (scd) is associated with milk yield.an association study between the milk yield trait and the stearoyl-coa desaturase (scd) polymorphism (g.133a > c) in italian mediterranean river buffalo was carried out. a full characterization of the river buffalo scd promoter region was presented. genotyping information was provided and a quick method for allelic discrimination was developed. the frequency of the c allele was 0·16. test-day (td) records (43 510) of milk production belonging to 226 lactations of 169 buffalo cows were analysed w ...201222994977
[screening and verification on characteristic differentially expressed genes of schistosoma japonicum from three reservoir hosts].to get the characteristic differentially expressed genes of schistosoma japonicum from three important reservoir hosts: yellow cattle, water buffalo and goat, so as to find the genetic markers to identify the various sources of the parasite reservoir hosts.201223012949
new method for the simultaneous identification of cow, sheep, goat, and water buffalo in dairy products by analysis of short species-specific mitochondrial dna targets.a novel method is presented here as an analytical tool for food control and authentication of dairy products manufactured from the milk of cow, sheep, goat, and buffalo. the method is based on multiplex polymerase chain reaction (pcr) of species-specific mitochondrial dna (mtdna) targets followed by fragment size analysis by capillary electrophoresis. the method includes (a) simultaneous detection of four species, (b) internal control for dna extraction and pcr, (c) mtdna as a target for pcr, (d ...201223025240
immunodetection of coproantigens for the diagnosis of amphistomosis in naturally infected indian water buffalo, bubalus bubalis.the infection of gastrointestinal helminths in livestock is routinely diagnosed by microscopical examination of faecal samples for the presence of ova/eggs but this approach becomes ineffective for the seasonally egg producing trematodes. therefore, an alternative approach to detect the coproantigens of liver and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer respectively, infecting indian water buffalo bubalus bubalis, was undertaken using elisa, immunodot and countercu ...201323036808
h19 gene methylation study in indian buffalo (bubalus bubalis).the methylation of dna at cytosine residues within cpg dinucleotides is associated with transcriptional repression and is implicated in maintaining genomic stability and also the silencing of repetitive elements. these imprinted genes are unique as they are expressed exclusively from one parental allele. the present study was carried out to detect methylation status in h19 gene promoter ctcf iii region in three indian buffalo breeds (jaffarabadi, surti and mehsani) by bisulfite sequencing. methy ...201323054415
toxicity of titanium oxide nanoparticles causes functionality and dna damage in buffalo (bubalus bubalis) sperm in vitro.the present study has examined the effect of different concentrations (1 μg/ml, 10 μg/ml and 100 μg/ml) of titanium oxide (tio2) nanoparticles (nps) (<100 nm) on viability, membrane integrity, capacitation status and dna integrity of buffalo spermatozoa. characterization of nps was done by the transmission electron microscopy (tem) and dynamic light scattering (dls). sperm chromatin dispersion (scd) test and acridine orange test (aot) were employed to detect dna fragmentation in sperm treated wi ...201423064765
ticks on buffaloes (bubalus bubalis) in the state of rio de janeiro, brazil.some tick species have been described parasitizing buffaloes, but reports from brazil are rare. this study aimed to ascertain the species of ticks that parasitize buffaloes in the state of rio de janeiro. seventeen farm properties were visited between june 2008 and december 2009, and buffaloes were inspected in relation to infestation. the ticks were identified and a semi-structured questionnaire on parasitism and treatment against ticks was filled out through interviews with the owners. the spe ...201223070448
detection of anti-borrelia burgdorferi antibodies in buffaloes (bubalus bubalis) in the state of pará, brazil.this study aimed to investigate the frequency of homologous antibodies of igg class against borrelia burgdorferi in buffaloes in the state of pará, brazil. blood serum samples from 491 buffaloes were analyzed by means of the indirect elisa test, using crude antigen produced from a cultivar of the north american strain g39/40 of b. burgdorferi. there were 412 positive samples (83.91%), and there was no statistically significant difference in the proportions of positive animals between the 81.69% ...201223070455
endometritis impairs luteal development, function, and nitric oxide and ascorbic acid concentrations in buffalo (bubalus bubalis).a vast majority of the world buffalo resource is concentrated in tropical and subtropical countries. apart from heat stress and poor nutritional availability, endometritis is one of the most commonly encountered reproductive problems limiting fertility and consequently productive potential of the species. as demonstrated recently, endometritis impairs growth and follicular fluid composition of the largest follicle in buffalo. in the present study, the effect of endometritis on luteal development ...201323070685
diversity of salmonella spp. serovars isolated from the intestines of water buffalo calves with gastroenteritis.salmonellosis in water buffalo (bubalus bubalis) calves is a widespread disease characterized by severe gastrointestinal lesions, profuse diarrhea and severe dehydration, occasionally exhibiting a systemic course. several salmonella serovars seem to be able to infect water buffalo, but salmonella isolates collected from this animal species have been poorly characterized. in the present study, the prevalence of salmonella spp. in water buffalo calves affected by lethal gastroenteritis was assesse ...201223098237
bovine papillomavirus type 2 infects the urinary bladder of water buffalo (bubalus bubalis) and plays a crucial role in bubaline urothelial carcinogenesis.bovine papillomavirus type 2 (bpv-2) has been shown to infect and play a role in urinary bladder carcinogenesis of buffaloes grazed on pastures with ferns from the marmara and black sea regions of turkey. bpv-2 dna has been found in both neoplastic and non-neoplastic lesions of the urinary bladder. furthermore, this virus may be a normal inhabitant of the urinary bladder since bpv-2 dna has also been detected in clinically normal buffaloes. the viral activation by fern immunosuppressant or carci ...201323100367
ultrastructural observation and gene expression profiling of schistosoma japonicum derived from two natural reservoir hosts, water buffalo and yellow cattle.water buffalo and yellow cattle are the two of the most important natural reservoir hosts for schistosoma japonicum in endemic areas of china, although their susceptibility differs, with water buffalo being less conducive to the growth and development of s. japonicum. results from the current study show that the general morphology and ultrastructure of adult schistosomes derived from the two hosts also differed. using high-throughput microarray technology, we also compared the gene expression pr ...201223110087
separation and quantification of water buffalo milk protein fractions and genetic variants by rp-hplc.a rp-hplc method, developed for the separation and quantification of the most common genetic variants of bovine milk proteins, was successfully applied to the analysis of water buffalo milk. all the most common buffalo casein and whey proteins fractions, as well as their genetic variants, were detected and separated simultaneously in 40 min. purified buffalo proteins were used as calibration standards and a total of 536 individual milk samples were analysed for protein composition. α(s1)-, α(s2) ...201323122071
characterization of the glutamate dehydrogenase activity of gigantocotyle explanatum and gastrothylax crumenifer (trematoda: digenea).glutamate dehydrogenase (gldh) (ec 1.4.1.3) is a ubiquitous enzyme, which is present at the protein and carbohydrate metabolism crossroads. the enzyme activity was investigated in biliary and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer, respectively, infecting the indian water buffalo bubalus bubalis. the enzyme activity was consistently higher in g. explanatum as compared to g. crumenifer, where nad(h) was utilized as coenzyme and the ph optima was recorded at 8. the ...200923129890
isolation of a gene encoding a cellulolytic enzyme from swamp buffalo rumen metagenomes and its cloning and expression in escherichia coli.ruminants are capable of hydrolyzing lignocellulosic residues to absorbable sugars by virtue of the microbial communities residing in their rumen. however, large sections of such microbial communities are not yet culturable using conventional laboratory techniques. therefore in the present study, the metagenomic dna of swamp buffalo (bubalus bubalis) rumen contents was explored using culture-independent techniques. the consensus regions of glycosyl hydrolase 5 (gh5) family of cellulases were use ...201223134306
in vitro culture and morphological characterization of prepubertal buffalo (bubalus bubalis) putative spermatogonial stem cell.spermatogonial stem cells (sscs) have the unique ability both to self-renew and to produce progeny that undergo differentiation to spermatozoa. the present study has been carried out to develop a method to purify and enrich the pure populations of spermatogonial stem cell like cells in buffalo.201223151879
glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (bubalus bubalis) bull spermatozoa.in this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (gsh) on buffalo (bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and dna integrity as well as in vivo fertility. semen from three nili-ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (37°c; 50×10(6)spermatozoaml(-1)) with experimental tris-citric acid extender containing 0, 0.5, 1.0, 1. ...201223153697
a novel procedure for precise quantification of schistosoma japonicum eggs in bovine feces.schistosomiasis japonica is a zoonosis with a number of mammalian species acting as reservoir hosts, including water buffaloes which can contribute up to 75% to human transmission in the people's republic of china. determining prevalence and intensity of schistosoma japonicum in mammalian hosts is important for calculating transmission rates and determining environmental contamination. a new procedure, the formalin-ethyl acetate sedimentation-digestion (fea-sd) technique, for increased visualiza ...201223166847
effects of glial cell line-derived neurotrophic factor, fibroblast growth factor 2 and epidermal growth factor on proliferation and the expression of some genes in buffalo (bubalus bubalis) spermatogonial cells.the present study evaluated the effects of glial cell line-derived neurotrophic factor (gdnf), fibroblast growth factor (fgf) 2 and epidermal growth factor (egf) on proliferation and the expression of some genes in spermatogonial cells. spermatogonial cells were isolated from prepubertal buffalo testes and enriched by double enzyme treatment, filtration through 80- and 60-μm nylon mesh filters, differential plating on lectin-coated dishes and percoll density gradient centrifugation. cells were t ...201323171731
isolation, characterization, and egfp expression in the buffalo (bubalus bubalis) mammary gland epithelial cell line.this study was aimed to establish a buffalo mammary epithelial cells (bumecs) line and maintain it for long-term by subculturing. bumecs isolated from lactating buffalo mammary glands were cultured on a collagen matrix gel. bumecs expressed significant amounts of the epithelial cell specific marker cytokeratin 18 as determined by immunohistochemistry. the bumecs displayed monolayer, cobble-stone morphology, and formed lumen-, dome-, and duct-like structures. furthermore, they were capable of syn ...201323180034
link between geographical origin and occurrence of brucella abortus biovars in cow and water buffalo herds.sixty-three brucella isolates from water buffaloes and cattle slaughtered within the italian national plan for brucellosis control were characterized by multiple-locus variable-number tandem repeat analysis (mlva). genotyping indicated a strong influence of geographic origin on the brucella abortus biovar distribution in areas where brucellosis is endemic and highlighted the importance of rigorous management procedures aimed at avoiding inter- and intraherd spreading of pathogens.201323183984
expression pattern of pluripotent markers in different embryonic developmental stages of buffalo (bubalus bubalis) embryos and putative embryonic stem cells generated by parthenogenetic activation.in this study, we describe the production of buffalo parthenogenetic blastocysts and subsequent isolation of parthenogenetic embryonic stem cell (pgesc)-like cells. pgesc colonies exhibited dome-shaped morphology and were clearly distinguishable from the feeder layer cells. different stages of development of parthenogenetic embryos and derived embryonic stem cell (esc)-like cells expressed key esc-specific markers, including oct-4, nanog, sox-2, foxd3, rex-1, stat-3, telomerase, nucleostemin, an ...201223194456
impact of buserelin acetate or hcg administration on day 12 post-ovulation on subsequent luteal profile and conception rate in buffalo (bubalus bubalis).the present study investigated the impact of gonadotropic hormone administration on day 12 post-ovulation on subsequent luteal profile and conception rate in buffaloes. all the buffaloes (n=48) were estrus synchronized by a synthetic analogue of prostaglandin f(2α) (pgf(2α)), administered 11 days apart, followed by insemination during mid to late estrus. to examine the effect of mid-luteal phase hormonal treatment, buffaloes were randomly divided into control (normal saline, n=14), d12-ba (buser ...201323201300
a high-resolution radiation hybrid map of the river buffalo major histocompatibility complex and comparison with bola.the major histocompatibility complex (mhc) in mammals codes for antigen-presenting proteins. for this reason, the mhc is of great importance for immune function and animal health. previous studies revealed this gene-dense and polymorphic region in river buffalo to be on the short arm of chromosome 2, which is homologous to cattle chromosome 23. using cattle-derived sts markers and a river buffalo radiation hybrid (rh) panel (bburh5000 ), we generated a high-resolution rh map of the river buffalo ...201323216319
investigation of transferability of bovinesnp50 beadchip from cattle to water buffalo for genome wide association study.cattle and water buffalo belong to the same subfamily bovinae and share chromosome banding and gene order homology. in this study, we used genome-wide illumina bovinesnp50 beadchip to analyze 91 dna samples from three breeds of water buffalo (nili-ravi, murrah and their crossbred with local guangxi buffalos in china), to demonstrate the genetic divergence between cattle and water buffalo through a large single nucleotide polymorphism (snp) transferability study at the whole genome level, and per ...201323232712
differential actions of proteinases and neuraminidase on mammalian erythrocyte surface and its impact on erythrocyte agglutination by concanavalin a.action of proteinases viz. trypsin and chymotrypsin, and neuraminidase on intact erythrocyte membrane proteins and glycophorins (sialoglycoproteins) exposed to cell surface and its impact on lectin (concanavalin a)-mediated agglutination were studied in homo sapiens (human), capra aegagrus hircus (goat) and bubalus bubalis (buffalo). membrane proteins and glycophorins analysis by sds-page as visualized by coomassie brilliant blue and periodic acid-schiff stains, respectively, and agglutination b ...201223255673
impact of gonadotropin supplementation on the expression of germ cell marker genes (mater, zar1, gdf9, and bmp15) during in vitro maturation of buffalo (bubalus bubalis) oocyte.the present study was designed to investigate whether gonadotropins [follicle-stimulating hormone (fsh) and luteinizing hormone (lh)] and buffalo follicular fluid (bff) supplementation in maturation medium influences the transcript abundance of germ cell marker genes [maternal antigen that embryos require (mater), zygote arrest 1 (zar1), growth differentiation factor 9 (gdf9), and bone morphogenetic protein 15 (bmp15)] mrna in buffalo (bubalus bubalis) oocytes. buffalo ovaries were collected fro ...201223263936
effect of season, late embryonic mortality and progesterone production on pregnancy rates in pluriparous buffaloes (bubalus bubalis) after artificial insemination with sexed semen.the use of sexed semen technology in buffaloes is nowadays becoming more and more accepted by farmers, to overcome the burden of unwanted male calves with related costs and to more efficiently improve production and genetic gain. the aim of this study was to verify the coupling of some variables on the efficiency of pregnancy outcome after deposition of sexed semen through ai. pluriparous buffaloes from two different farms (n = 152) were screened, selected, and subjected to ovsynch protocol for ...201223265928
a complementary diagnosis of naturally occurring tuberculosis in water buffaloes (bubalus bubalis) in rio de janeiro using a mpb70-elisa, brazil.as tuberculosis is still a worldwide infection and buffalo breeding represents an important economic activity in various countries, the purpose of this study was to employ an enzyme-linked immunosorbent assay (elisa) using mpb70 as a capture antigen for the diagnosis of naturally occurring tuberculosis in water buffaloes in brazil. after the introduction of newly acquired cattle onto a tuberculosis (tb) free farm, an outbreak of tb was recorded in a mixed herd comprising water buffaloes (21) and ...201223274697
cryopreservation of zona-free cloned buffalo (bubalus bubalis) embryos: slow freezing vs open-pulled straw vitrification.this study was carried out to compare the post-thaw cryosurvival rate and the level of apoptosis in vitro produced zona-free cloned buffalo blastocysts subjected to slow freezing or vitrification in open-pulled straws (ops). zona-free cloned embryos produced by handmade cloning were divided into two groups and were cryopreserved either by slow freezing or by vitrification in ops. cryosurvival of blastocysts was determined by their re-expansion rate following post-thaw culture for 22-24 h. the po ...201323281817
effect of the stage of estrous cycle on follicular population, oocyte yield and quality, and biochemical composition of serum and follicular fluid in anatolian water buffalo.this study was designed to investigate the effects that the different stages of the estrous cycle had on the number of surface ovarian follicles and oocyte yield and quality of anatolian water buffalo during peak breeding season. assessments were made on the basis of ovarian morphology, serum and follicular fluid concentrations of variety of biochemical parameters. following slaughter, blood samples were collected from each animal. the stage of estrous cycle was classified as either the luteal o ...201323317849
effect of tgf-β1 superfamily members on survival of buffalo (bubalus bubalis) embryonic stem-like cells.this study examined the effects of supplementation of es-like cell culture medium with bone morphogenetic protein (bmp)-4 (0, 10, 20 or 100 ng/ml) or noggin (250, 500 or 750 ng/ml) or tgf-β1 (0, 0.1, 1 or 10 ng/ml) or sb431542 (0, 10, 25 or 50 μm), an inhibitor of tgf-β1 signalling, on survival, colony area and expression level of pluripotency genes in buffalo es-like cells at passage 40-80, under different culture conditions. bmp-4 supplementation significantly reduced (p < 0.05) colony surviva ...201323320894
bubaline herpesvirus 1 associated with abortion in a mediterranean water buffalo.during routine analysis of water buffalo foetuses, one sample was positive for herpesvirus and negative to all the other abortive agents investigated. sequencing of the herpesvirus glycoprotein e gene identified the virus as bubaline herpesvirus 1, showing few differences with the published sequences. this represents the first finding of bubaline herpesvirus in a water buffalo foetus associated with abortion.201323332497
in silico mining of putative microsatellite markers from whole genome sequence of water buffalo (bubalus bubalis) and development of first buffsatdb.though india has sequenced water buffalo genome but its draft assembly is based on cattle genome btau 4.0, thus de novo chromosome wise assembly is a major pending issue for global community. the existing radiation hybrid of buffalo and these reported str can be used further in final gap plugging and "finishing" expected in de novo genome assembly. qtl and gene mapping needs mining of putative str from buffalo genome at equal interval on each and every chromosome. such markers have potential rol ...201323336431
clinical and molecular study of a new form of hereditary myotonia in murrah water buffalo.hereditary myotonia caused by mutations in clcn1 has been previously described in humans, goats, dogs, mice and horses. the goal of this study was to characterize the clinical, morphological and genetic features of hereditary myotonia in murrah buffalo. clinical and laboratory evaluations were performed on affected and normal animals. clcn1 cdna and the relevant genomic region from normal and affected animals were sequenced. the affected animals exhibited muscle hypertrophy and stiffness. myoton ...201323339992
identification of suitable housekeeping genes for normalization of quantitative real-time pcr data during different physiological stages of mammary gland in riverine buffaloes (bubalus bubalis).gene expression analysis unravels the complex changes or relations at transcriptomic level. to nullify all type of errors that can be incorporated during any stage of rna extraction into cdna synthesis and for reliable results, the data obtained from qpcr have to be normalized using the appropriate/suitable housekeeping genes (hkgs). unfortunately, till date, no such hkg has been reported for bubaline mammary gland. the objective of the present study was thus to identify and validate the potenti ...201323363300
amount of mrna and localization of vascular endothelial growth factor and its receptors in the ovarian follicle during estrous cycle of water buffalo (bubalus bubalis).the objective of the present study was to characterize the temporal patterns of gene expression for vascular endothelial growth factors (vegf) and vegf receptors during ovarian follicular growth, development and maturation in buffalo (bubalus bubalis). follicles were classified into four groups according to size and the concentration of estradiol-17β (e2) in follicular fluid (ff): group i (small), 4-6mm diameter, e2>0.5ng/ml of ff; group ii (medium), 7-9mm, e2=0.5-5ng/ml; group iii (large), 10-1 ...201323375984
molecular characterization of trypanosoma evansi isolates from water buffaloes (bubalus bubalis) in the philippines.trypanosoma evansi infection in the philippines is frequently reported to affect the country's livestock, particularly, the buffaloes. to assess the prevalence and intraspecific diversity of t. evansi in the country, blood samples from water buffaloes in different geographical regions were collected during an outbreak. t. evansi was detected in all 79 animals tested using pcr targeting the rotat 1.2 vsg gene. sequencing of the rdna complete internal transcribed spacer (its) region including the ...201323377905
analysis and functional annotation of expressed sequence tags of water buffalo.an elucidated genome of domestic livestock river buffalo will contribute enormously to economy and better understanding of genome evolution as well. an attempt is made to obtain genomic information on buffalo, based on total expressed sequence tags (ests) of bubalus bubalis available in public domain. these ests were annotated and classified into 15 different functional categories based on their homology to the known proteins. interestingly, 41.79% of the contigs were found to be buffalo specifi ...201323394367
a distinct genetic population of gongylonema pulchrum from water buffaloes in nepal.whole-length esophagi of 111 murrah cross water buffaloes (bubalus bubalis) were collected in the kathmandu and chitwan districts of nepal from december 2009 to february 2010. gullet worms showing a typical epithelium-dwelling character were detected in 13 of 53 (24.5%) buffaloes in kathmandu and in 5 of 58 (8.6%) buffaloes in chitwan. the worms' morphology and measurements were identical to those of gongylonema pulchrum molin, 1857, except for the length of the left spicules relative to the bod ...201323421498
comparison of acute infection of calves exposed to a high-virulence or low-virulence bovine viral diarrhea virus or a hobi-like virus.to compare acute infection of cattle exposed to a high-virulence (hv) bovine viral diarrhea virus (bvdv), low-virulence (lv) bvdv, or hobi-like virus.201323438120
presence of campylobacter and arcobacter species in in-line milk filters of farms authorized to produce and sell raw milk and of a water buffalo dairy farm in italy.the objectives of this study were to investigate the presence of campylobacter spp. and arcobacter spp. in dairy herds authorized for the production and sale of raw milk and in a water buffalo dairy farm, and to test the antimicrobial susceptibility of the isolates. a total of 196 in-line milk filters were collected from 14 dairy farms (13 bovine and 1 water buffalo) for detection of campylobacter spp. and arcobacter spp. by microbiological culture. for each farm investigated, 1 isolate for each ...201323453517
cultural legacies, fire ecology, and environmental change in the stone country of arnhem land and kakadu national park, australia.we use the fire ecology and biogeographical patterns of callitris intratropica, a fire-sensitive conifer, and the asian water buffalo (bubalus bubalis), an introduced mega-herbivore, to examine the hypothesis that the continuation of aboriginal burning and cultural integration of buffalo contribute to greater savanna heterogeneity and diversity in central arnhem land (cal) than kakadu national park (knp). the 'stone country' of the arnhem plateau, extending from knp to cal, is a globally renowne ...201323467505
polymorphisms in the ghrelin gene and their associations with milk yield and quality in water buffaloes.ghrelin is a gastrointestinal hormone that acts in releasing growth hormone and influences the body general metabolism. it has been proposed as a candidate gene for traits such as growth, carcass quality, and milk production of livestock because it influences feed intake. in this context, the aim of this study was to verify the existence of polymorphisms in the ghrelin gene and their associations with milk, fat and protein yield, and percentage in water buffaloes (bubalus bubalis). a group of 24 ...201323497998
dna-based authentication method for detection of yak (bos grunniens) in meat products.a taqman probe real-time pcr method was developed for rapid detection of yak component in raw and cooked meat products. specific primers and taqman probes of yak (bos grunniens) were designed in the cytochrome b gene. the specificity of the method was evaluated using pure meat of eight yak breeds (jiulong, qinghai plateau, maiwa, gannan, bazhou, sibu, zhongdian, and jiali) samples and nine non-bos grunniens animals (sheep, goat, pig, chicken, cattle, water buffalo, donkey, horse, and rabbit). dn ...201323513969
minimum number of spermatozoa per dose in mediterranean italian buffalo (bubalus bubalis) using sexed frozen semen and conventional artificial insemination.in buffaloes, ai with sexed semen is not fully optimized, and the procedure has only been performed using the approach currently in use for cattle. the objective of the present work was to compare the pregnancy rates in mediterranean italian buffalo cows inseminated with sexed frozen-thawed semen at 2, 4, 6, and 8 million sperm per dose, using the ovsynch protocol and conventional ai at a fixed time. fresh ejaculates from three buffalo bulls were processed according to beltsville sperm sorting t ...201323523175
isolation of arcobacter species in water buffaloes (bubalus bubalis).this is the first report of arcobacter spp. in rectal fecal samples from healthy water buffaloes (bubalus bubalis) reared on a dairy farm. arcobacter species were isolated after enrichment, and isolates were identified at species level by multiplex-polymerase chain reaction assay. thirty samples were examined and arcobacter spp. were isolated from 96.7% of water buffaloes tested: 38 arcobacter spp. isolates were obtained, with a. cryaerophilus as the dominant species followed by a. butzleri and ...201323536981
effect on quarter milk somatic cell count and antimicrobial susceptibility of staphylococcus rostri causing intramammary infection in dairy water buffaloes.in many parts of the world, coagulase-negative staphylococci (cns) are the predominant cause of intramammary infections (imi) in dairy cows and in water buffaloes, as well. a longitudinal field study was carried out on one well-managed dairy water buffalo herd to determine the prevalence and distribution of cns and a recently described cns-species, staphylococcus rostri, in milk samples to explore its relevance for buffaloes' udder health throughout lactation, and to gain insight into the suscep ...201323548306
expression and localization of vascular endothelial growth factor and its receptors in the corpus luteum during oestrous cycle in water buffaloes (bubalus bubalis).the aim of this study was to document the expression and localization of vegf system comprising of vegf isoforms (vegf 120, vegf 164 and vegf 188) and their receptors (vegfr1 and vegfr2) in buffalo corpus luteum (cl) obtained from different stages of the oestrous cycle. real-time rt-pcr (qpcr), western blot and immunohistochemistry were applied to investigate mrna expression, protein expression and localization of examined factors. in general, all the components of vegf system (the vegf isoforms ...201323551326
faecal chemical cues in water buffalo that facilitate estrus detection.chemo-signals are among the reliable non-invasive methods for estrus detection in mammals. water buffalo is a silent heat animal and, hence, there is search for chemo-signals which would be effective non-invasive indicators of estrus state. we analyzed the faecal chemical cues during the estrous cycle in buffalo and to find the estrus-specific faecal volatile compounds adopting bull behavior assay. the faecal samples were collected at three phases of the estrous cycle (i.e., proestrus, estrus an ...201323570909
proteomic profiles of the embryonic chorioamnion and uterine caruncles in buffaloes (bubalus bubalis) with normal and retarded embryonic development.the aim of this study was to compare the proteome profiles of the chorioamnion and corresponding caruncle for buffalo embryos that had either normal or retarded development on day 25 after artificial insemination (ai). in experiment 1, embryos that were to subsequently undergo late embryonic mortality had a smaller width on day 25 after ai than embryos associated with pregnancy on day 45 after ai. in experiment 2, 25 italian mediterranean buffaloes underwent transrectal ultrasonography on day 25 ...201323575152
effect of different thawing rates on post-thaw viability, kinematic parameters and chromatin structure of buffalo (bubalus bubalis) spermatozoa.the aim of the present study was to evaluate three thawing rates on the post thaw motility, viability and chromatin structure of buffalo semen frozen in 0.5-ml straws.201323577311
effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (bubalus bubalis) embryos produced in vitro.for investigating the effects of physiologically relevant heat shock, buffalo oocytes/embryos were cultured at 38.5°c (control) or were exposed to 39.5°c (group ii) or 40.5°c (group iii) for 2 h once every day throughout in vitro maturation (ivm), fertilization (ivf) and culture (ivc). percentage of oocytes that developed to 8-cell, 16-cell or blastocyst stage was lower (p < 0.05) and the number of apoptotic nuclei was higher (p < 0.05) for group iii > group ii > controls. at both 8-16-cell and ...201323581430
comparative evaluation of oral and dermal cypermethrin exposure on antioxidant profile in bubalus bubalis.cypermethrin, a type ii synthetic pyrethroid insecticide, @ 0.5mg/kg/day for 14 consecutive weeks produced mild signs of toxicity in buffalo calves. significant changes were observed in various antioxidant parameters in blood. there was a marked increase in the extent of lipid peroxidation (33.9%) and enzymic activity of glutathione peroxidase (6.7%), superoxide dismutase (35.0%), catalase (43.7%), glutathione-s-transferase (64.4%), glutathione reductase (36.7%) and glucose-6-phosphate dehydroge ...201323623842
purification of pregnancy-associated glycoproteins from late-pregnancy bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females.pregnancy-associated glycoproteins (pags) were first described as placental antigens present in the blood serum of the mother soon after implantation. here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbpags). a specific radioimmunoassay (ria) was developed for early pregnancy diagnosis in buffalo species.201323634647
antibodies to schmallenberg virus in domestic livestock in turkey.this, partly retrospective study, was designed to determine the seroprevalence of schmallenberg virus (sbv), a new orthobunyavirus first reported in germany in late 2011, in domestic ruminants from the middle black sea, west, and southeast regions of turkey. an indirect enzyme-linked immunosorbent assay was used to screen serum samples collected from slaughterhouse animals between 2006 and 2013. the overall seroprevalence was 335/1,362 (24.5 %) with 325/816 (39.8 %), 5/307 (1.6 %), 3/109 (2.8 %) ...201323645515
physical mapping of 20 unmapped fragments of the btau_4.0 genome assembly in cattle, sheep and river buffalo.the recent advances in sequencing technology and bioinformatics have revolutionized genomic research, making the decoding of the genome an easier task. genome sequences are currently available for many species, including cattle, sheep and river buffalo. the available reference genomes are very accurate, and they represent the best possible order of loci at this time. in cattle, despite the great accuracy achieved, a part of the genome has been sequenced but not yet assembled: these genome fragme ...201323652984
wnt3a signalling pathway in buffalo (bubalus bubalis) embryonic stem cells.the aim of this study was to investigate the transcriptional profile and role of wnt3a signalling in maintaining buffalo embryonic stem (es) cells in a pluripotent state and in the induction of their differentiation. es cells were derived from embryos produced by in vitro fertilisation (iesc), parthenogenesis (pesc) and hand-made cloning (cesc). the expression of wnt3a, its receptors and intermediate signalling pathways were found to be conserved in es cells derived from the three different sour ...201423656691
effects of a single nucleotide polymorphism in the leptin gene on the productive traits of dairy buffaloes (bubalus bubalis).the gene responsible for coding the leptin hormone has been associated with productive and reproductive traits in cattle. in dairy cattle, different polymorphisms found in the leptin gene have been associated with several traits of economic interest, such as energy balance, milk yield and composition, live weight, fertility and dry matter consumption. the aim of this study was to detect genetic variability in the leptin gene of buffaloes and to test possible associations with milk yield, fat and ...201323661024
seroprevalence of trypanosoma evansi infection in water buffaloes from the mountainous region of north vietnam and effectiveness of trypanocidal drug treatment.water buffalo (wb) is an important domestic animal in vietnam. this study utilized a card agglutination test to investigate seroprevalence of surra in wb population. sera were collected from 585 wb from 4 different regions in cao bang and thai nguyen provinces. among them, 131 samples (22.4%) were positive for surra. the highest prevalence (24.6%) was found among 3 to 5 years old wb. buffaloes less than 3 years old had the lowest prevalence (15.6%). among 27 abortion cases, 9 wb (33.3%) were sur ...201323665513
importance of the horse and financial impact of equine trypanosomiasis on cattle raising in venezuela.in venezuela, horses are indispensable for extensive cattle raising, and extensive cattle raising prevails in all regions. this determines the numerical relationship between horses and cattle (r = 0.93) to be relatively constant nationwide. at regional level, the average extension of cattle ranches varies greatly. however, in relation to the area covered by pastures, the numbers of horses (r = 0.95) and cattle (r = 0.93) are relatively uniform nationwide. water buffalo occupy small fractions of ...201323666515
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