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stenotrophomonas maltophilia in cystic fibrosis: guilty or innocent? 201121471053
induction of aromatic ring: cleavage dioxygenases in stenotrophomonas maltophilia strain kb2 in cometabolic systems.stenotrophomonas maltophilia kb2 is known to produce different enzymes of dioxygenase family. the aim of our studies was to determine activity of these enzymes after induction by benzoic acids in cometabolic systems with nitrophenols. we have shown that under cometabolic conditions kb2 strain degraded 0.25-0.4 mm of nitrophenols after 14 days of incubation. simultaneously degradation of 3 mm of growth substrate during 1-3 days was observed depending on substrate as well as cometabolite used. fro ...201121475727
changes in bacterial flora of japanese cabbage during growth and potential source of flora.bacterial flora of cabbage were identified and enumerated during various stages of growth, and the potential sources of contamination in the field were determined. bacterial counts increased from below the level of detection (2.4 log cfu/g) on seeds to 2.5 to 5.7 log cfu/g on seedlings. after transplanting, the counts of mesophilic aerobic bacteria on leaves decreased and then increased to 5.7 log cfu/g on outer leaves, 5.0 log cfu/g on middle leaves, and 3.0 log cfu/g on inner leaves at the har ...201121477482
diversity of culturable chitinolytic bacteria from rhizospheres of agronomic plants in japan.a total of 100 isolates of chitinolytic bacteria were obtained from the rhizospheres of various agronomic plants, and the 16s rrna gene sequences of these isolates were determined. phylogenetic analyses revealed that 81 isolates belonged to the classes betaproteobacteria (39 isolates) and gammaproteobacteria (42 isolates). of the remaining 19 isolates, 16 belonged to the phylum firmicutes. clustering analysis identified 6 and 3 operational taxonomic units (otus) in gammaproteobacteria and betapr ...201121487197
pathogen and autoantigen homologous regions within the cystic fibrosis transmembrane conductance regulator (cftr) protein suggest an autoimmune treatable component of cystic fibrosis.the cystic fibrosis transmembrane conductance regulator (cftr) chloride channel provides the glutathione and hypochlorous acid necessary for bactericidal/viricidal actions. cftr mutations block these effects, diminishing pathogen defence and allowing extracellular pathogen accumulation, where antibody encounter is likely. kegg pathway analysis of the cftr interactome shows that cftr is involved in pathogen entry pathways and immune defence as well as in pathways relevant to comorbid conditions ( ...201121496124
establishment of an arabinose-inducible system in stenotrophomonas maltophilia.a pbbad22t-derived conditioned arabinose (ara)-inducible expression system was evaluated in stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). s. maltophilia cannot grow well when ara is the sole available carbon source. the induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xyle gene fusion construct, pbxyle, to monitor the expression of ...201121503736
stenotrophomonas maltophilia: emerging disease patterns and challenges for treatment.stenotrophomonas maltophilia is a ubiquitous organism associated with opportunistic infections. in the immunocompromised host, increasing prevalence and severity of illness is observed, particularly opportunistic bloodstream infections and pneumonia syndromes. in this article, the classification and microbiology are outlined, together with clinical presentation, outcomes and management of infections due to s. maltophilia. although virulence mechanisms and the genetic basis of antibiotic resistan ...201121504403
revisited distribution of nonfermenting gram-negative bacilli clinical isolates.nonfermenting gram-negative bacilli (nf-gnb) are ubiquitous environmental opportunistic bacteria frequently misidentified by conventional phenotypic methods. the aim of this study was to determine the distribution of nf-gnb species by 16 s rrna gene sequencing (used as reference method) and to compare performances of biochemical tests and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof-ms). from nine french hospitals, 188 nf-gnb isolates (except p. aerugin ...201121509476
immunoassay method to check the flagellin mediated binding of stenotrophomonas maltophilia to polystyrene.plate count and spectrophotometric methods have been used to asses the ability of an organism to attach to different surfaces and form biofilms. in the present study we report a highly sensitive, specific and quick method to check the role of flagellin in bacterial adhesion to polystyrene. flagellin from stenotrophomonas maltophilia showed high affinity for polystyrene (p < 0.05), which decreased on pretreatment offlagellin with anti-flagellin in a dilution dependent manner. in an enzyme immunoa ...201121513218
microbial isolation and emergence of antimicrobial resistance associated with tigecycline usage.background: with the increasing experience of tigecycline usage, its ecological impact on microorganisms raises concerns but remains unknown. we aimed to analyze the difference in microorganisms isolated before, during, and after tigecycline usage and their susceptibility to antimicrobial agents. methods: between july 2008 and august 2009, 66 patients who received tigecycline monotherapy for more than 2 days at a taiwan medical center were enrolled. antimicrobial susceptibility testing was perfo ...201121524975
degradation of cyhalofop-butyl (cyb) by pseudomonas azotoformans strain qdz-1 and cloning of a novel gene encoding cyb-hydrolyzing esterase.cyhalofop-butyl (cyb) is a widely used aryloxyphenoxy propanoate (aopp) herbicide for control of grasses in rice fields. five cyb-degrading strains were isolated from rice field soil and identified as agromyces sp., stenotrophomonas sp., aquamicrobium sp., microbacterium sp., and pseudomonas azotoformans ; the results revealed high biodiversity of cyb-degrading bacteria in rice soil. one strain, p. azotoformans qdz-1, degraded 84.5% of 100 mg l(-1) cyb in 5 days of incubation in a flask and util ...201121534595
stenotrophomonas maltophilia strains replicate and persist in the murine lung, but to significantly different degrees.the environmental bacterium stenotrophomonas maltophilia is increasingly described as a multidrug-resistant pathogen of humans, being associated with pneumonia, among other things. but, the degree to which s. maltophilia is capable of replicating in a mammalian host has been an issue of controversy. using a model of intranasal inoculation into adult a/j mice, we now document that s. maltophilia strain k279a, the clinical isolate of s. maltophilia whose complete genome sequence was recently deter ...201121546584
adhesion mechanisms of plant-pathogenic xanthomonadaceae.the family xanthomonadaceae is a wide-spread family of bacteria belonging to the gamma subdivision of the gram-negative proteobacteria, including the two plant-pathogenic genera xanthomonas and xylella, and the related genus stenotrophomonas. adhesion is a widely conserved virulence mechanism among gram-negative bacteria, no matter whether they are human, animal or plant pathogens, since attachment to the host tissue is one of the key early steps of the bacterial infection process. bacterial att ...201121557058
complicated features in a young child with influenza b virus pneumonia and co-infection with stenotrophomonas maltophilia.a 3·5-year-old child with influenza b virus pneumonia developed pneumomediastinum and subcutaneous emphysema on the 3rd day of illness. bronchoscopy demonstrated obstruction of the left main bronchus by mucopurulent sputum. culture of the broncho-alveolar lavage yielded stenotrophomonas maltophilia. after the respiratory complications resolved (11 days), the patient developed neurological symptoms and was diagnosed as acute disseminated encephalomyelitis (adem). stenotrophomonas maltophilia was ...201121575322
predictors of catheter-related gram-negative bacilli bacteraemia among cancer patients.clin microbiol infect abstract: gram-negative bacillary bacteraemia (gnb) is associated with high morbidity and mortality among cancer patients. we conducted this study to determine the risk factors that may predict the catheter as the source of gnb in cancer patients. from july 2005 to december 2006 all 266 cancer patients with gnb and central venous catheters (cvcs) at the university of texas m. d. anderson cancer centre in houston, were classified as catheter-related bloodstream infection (cr ...201121595787
risk factors and clinical characteristics of stenotrophomonas maltophilia infections in neonates.background: the aim of this study was to review the risk factors and clinical, bacteriological, and epidemiological characteristics of stenotrophomonas maltophilia infections in our neonatal intensive care unit. methods: a retrospective matched case-control study was performed by comparing 23 cases of s maltophilia with 45 controls to identify the potential risk factors. to identify the case patients, the admission and medical records of patients in the neonatal intensive care unit and records f ...201121606009
case report: spontaneous stenotrophomonas maltophilia keratitis in a diabetic patient.objectives:: to report a rare case of spontaneous stenotrophomonas maltophilia keratitis in a diabetic patient. methods:: a case report of this rare ulcerative keratitis case with an extensive review of the literature. results:: the patient's corneal infiltrate was successfully cultured and a rare ocular organism identified. the patient's presentation and course and the response to empiric treatment are discussed. conclusions:: consideration of the antibiotic combination chosen here can be consi ...201121617538
intragenomic and intraspecific heterogeneity of the 16s rrna gene in seven bacterial species from the respiratory tract of cystic fibrosis patients assessed by pcr-temporal temperature gel electrophoresis.16s rrna gene-based cultivation-independent methods are increasingly used to study the diversity of microbiota during health and disease. one bias of these methods is the variability of 16s rrna gene that may exist among strains of a same species (intraspecific heterogeneity) or between rrs copies in a genome (intragenomic heterogeneity). we evaluated the level of intraspecific and intragenomic 16s rdna variability in seven species frequently encountered in respiratory tract samples in cystic fi ...201121621347
a novel highly charged exopolysaccharide produced by two strains of stenotrophomonas maltophilia recovered from patients with cystic fibrosis.stenotrophomonas maltophilia is a non-fermenting gram-negative microorganism capable of causing chronic pulmonary infection in cystic fibrosis patients and its ability to form biofilms on polystyrene and glass surfaces, as well as on cystic fibrosis-derived bronchial epithelial ib3-i cells was recently demonstrated. the latter evidence might explain the power of s. maltophilia to produce persistent lung infections, despite intensive antibiotic treatment. in addition to being important components ...201121636078
an unknown complication of peripherally inserted central venous catheter in a patient with ventricular assist device.we report an unknown complication of peripherally inserted central venous catheter in a patient with ventricular assist device. this rare complication led to the failure of the right ventricular assist device, which could be detrimental in patients with dilated cardiomyopathy.201121636933
physiological traits of the symbiotic bacterium teredinibacter turnerae isolated from the mangrove shipworm neoteredo reynei.nutrition in the teredinidae family of wood-boring mollusks is sustained by cellulolytic/nitrogen fixing symbiotic bacteria of the teredinibacter clade. the mangrove teredinidae neoteredo reynei is popularly used in the treatment of infectious diseases in the north of brazil. in the present work, the symbionts of n. reynei, which are strictly confined to the host's gills, were conclusively identified as teredinibacter turnerae. symbiont variants obtained in vitro were able to grow using casein a ...200921637522
stenotrophomonas maltophilia causing early onset neonatal sepsis.stenotrophomonas maltophilia, a multi drug resistant non fermenting gram negative bacillus is an increasingly common nosocomial pathogen, especially in intensive care units. comparatively few cases of infection have been reported in neonatal population. we present two cases of early onset neonatal sepsis due to s.maltophilia and a brief review of documented isolation in neonates.201121654005
a comparative study of biodegradation of vinyl acetate by environmental strains.four gram-negative strains, e3_2001, ec1_2004, ec3_3502 and ec2_3502, previously isolated from soil samples, were subjected to comparative studies in order to select the best vinyl acetate degrader for waste gas treatment. comparison of biochemical and physiological tests as well as the results of fatty acids analyses were comparable with the results of 16s rrna gene sequence analyses. the isolated strains were identified as pseudomonas putida ec3_2001, pseudomonas putida ec1_2004, achromobacter ...201121654921
fatal hemorrhagic pneumonia caused by stenotrophomanas maltophilia in a patient with non-hodgkin lymphoma.stenotrophomonas maltophilia is increasingly emerging as a multiresistant pathogen in the hospital environment. in immunosuppressed patients, this bacterium may cause severe infections associated with sepsis and multiple organ dysfunction. we report on a 57-year-old woman treated with intensive chemotherapy for non-hodgkin lymphoma who developed severe neutropenia, hemorrhagic pneumonia, and acute respiratory failure, which led to her death within 36 h of onset of pneumonia. postmortem examinati ...201121656194
culture-dependent and -independent molecular analysis of the bacterial community within uranium ore.the bacterial community structure within a uranium ore was investigated using culture-dependent and -independent clone library analysis and denaturing gradient gel electrophoresis of 16s rrna genes. the major aerobic heterotrophic bacteria were isolated and identified, and their resistance to uranium and other heavy metals was characterized. together with near neutral ph, moderate organic carbon content, elevated u and other heavy metals (v, ni, mn, cu, etc.), the ore showed high microbial count ...201121656801
diversity and phylogeny of bacteria on zimbabwe tobacco leaves estimated by 16s rrna sequence analysis.microorganisms play important roles in the tobacco aging process. however, microbial communities on flue-cured tobacco leaves (fctl) remain largely unknown. in this study, the total microbial genomic dna of unaged and aging fctl from zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16s rrna gene libraries. eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging fctl, re ...201121660545
antimicrobial susceptibilities of commonly encountered bacterial isolates to fosfomycin determined by agar dilution and disk diffusion methods.we studied the antimicrobial activity of fosfomycin against 960 strains of commonly encountered bacteria associated with urinary tract infection using standard agar dilution and disk diffusion methods. species studied included 3 common species of enterobacteriaceae, pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia; methicillin-susceptible and -resistant staphylococcus aureus; and vancomycin-susceptible and resistant enterococcus faecalis and e. faecium. mics and ...201121670185
phylogenetic analysis of culturable marine bacteria in sediments from the south korean yellow sea.biogeochemical and microbiological characterization of marine sediments taken from the yellow sea of south korea was carried out. one hundred and thirty six bacterial strains were isolated, characterized and phylogenetic relationship was evaluated. the gene sequences of 16s rdna regions were examined to study the phylogenetic analysis of bacterial community in the marine sediments. among 136 isolates, 5 bacterial isolates were identified as novel members, remaining 131 isolates were fall into 5 ...201121675219
mucosal surveillance cultures in predicting gram-negative late-onset sepsis in neonatal intensive care units.this study aimed to examine the spectrum and time course of gut and nasopharyngeal colonization with gram-negative micro-organisms, and to define the value of surveillance cultures in predicting late-onset sepsis in neonates admitted to neonatal intensive care units. nasopharyngeal and rectal swabs were collected on admission and twice weekly thereafter in 278 neonates admitted within the first 72-áh of life with risk factors of early-onset sepsis. sterile body fluid cultures were obtained on ad ...201121684633
genetic diversity and phylogeny of antagonistic bacteria against phytophthora nicotianae isolated from tobacco rhizosphere.the genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by boxair-pcr, 16s-rflp, 16s rrna sequence homology and phylogenetic analysis methods. these studies revealed that 4.01% of the 6652 tested had some inhibitory activity against phytophthora nicotianae. boxair-pcr analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. a total of 25 16s-rflp patterns were iden ...201121686169
resolving the phylogenetic and taxonomic relationship of xanthomonas and stenotrophomonas strains using complete rpob gene sequence. 201121686310
nile red detection of bacterial hydrocarbons and ketones in a high-throughput format.a method for use in high-throughput screening of bacteria for the production of long-chain hydrocarbons and ketones by monitoring fluorescent light emission in the presence of nile red is described. nile red has previously been used to screen for polyhydroxybutyrate (phb) and fatty acid esters, but this is the first report of screening for recombinant bacteria making hydrocarbons or ketones. the microtiter plate assay was evaluated using wild-type and recombinant strains of shewanella oneidensis ...201121712420
diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze).the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ...201121715960
inactivation of mrca gene derepresses the basal-level expression of l1 and l2 {beta}-lactamases in stenotrophomonas maltophilia.objectives to characterize the relationship between inactivation of the mrca gene and +¦-lactamase expression and +¦-lactams resistance in stenotrophomonas maltophilia kj and to investigate the involvement of ampr, ampn-ampg, ampd(i) and crebc in this. methods the mrca deletion mutant kj+ömrca was constructed to investigate the role of this putative penicillin-binding protein 1a (pbp1a) in +¦-lactamase expression and +¦-lactam resistance. the +öampr, +öampng, +öampdi and +öcrebc alleles were int ...201121719470
phenotypic and genotypic characterization of stenotrophomonas maltophilia isolates from patients with cystic fibrosis: genome diversity, biofilm formation, and virulence.abstract:201121729271
acceleration of nonylphenol and 4-tert-octylphenol degradation in sediment by phragmites australis and associated rhizosphere bacteria.we investigated biodegradation of technical nonylphenol (tnp) in phragmites australis rhizosphere sediment by conducting degradation experiments using sediments spiked with tnp. accelerated tnp removal was observed in p. australis rhizosphere sediment, whereas tnp persisted in unvegetated sediment without plants and in autoclaved sediment with sterile plants, suggesting that the accelerated tnp removal resulted largely from tnp biodegradation by rhizosphere bacteria. three bacterial strains, ste ...201121736332
the binding of triclosan to smet, the repressor of the multidrug efflux pump smedef, induces antibiotic resistance in stenotrophomonas maltophilia.the wide utilization of biocides poses a concern on the impact of these compounds on natural bacterial populations. furthermore, it has been demonstrated that biocides can select, at least in laboratory experiments, antibiotic resistant bacteria. this situation has raised concerns, not just on scientists and clinicians, but also on regulatory agencies, which are demanding studies on the impact that the utilization of biocides may have on the development on resistance and consequently on the trea ...201121738470
an mlsa-based online scheme for the rapid identification of stenotrophomonas isolates.an online scheme to assign stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (mlsa), which is based on the dna sequencing of selected fragments of the housekeeping genes atp synthase alpha subunit (atpa), the recombination repair protein (reca), the rna polymerase alpha subunit (rpoa) and the excision repair beta subunit (uvrb). this mlsa-based scheme was validated using eight of the 10 stenotrophomonas species that have been previously described. t ...201121739025
testosterone-mineralizing culture enriched from swine manure: characterization of degradation pathways and microbial community composition.environmental releases and fate of steroid sex hormones from livestock and wastewater treatment plants are of increasing regulatory concern. despite the detection of these hormones in manures, biosolids, and the environment, little attention has been paid to characterization of fecal bacteria capable of hormone degradation. the enrichments of (swine) manure-borne bacteria capable of aerobic testosterone degradation were prepared and the testosterone mineralization pathway was elucidated. six dna ...201121740029
infection in immunocompromised hosts and the organ transplant recipient -essentials. 201121748845
c-s targeted biodegradation of dibenzothiophene by stenotrophomonas sp. nisoc-04.crude oil-contaminated soil samples were gathered across khuzestan oilfields (national iranian south oil company, nisoc) consequently experienced a screening procedure for isolating c-s targeted dibenzothiophene-biodegrading microorganisms with previously optimized techniques. among the isolates, a bacterial strain was selected due to its capability of biodegrading dibenzothiophene in a c-s targeted manner in aqueous phases and medium mostly consisting of separately biphasic water-gasoline. the ...201121750993
stenotrophomonas maltophilia in cystic fibrosis: improved detection by the use of selective agar and evaluation of antimicrobial resistance.background: we implemented a selective medium for improved detection of stenotrophomonas maltophilia in sputum samples from cf patients. we also performed antimicrobial susceptibility testing with eight antibiotics. methods: a total of 623 consecutive sputum samples from 165 cf patients in a german cf center were cultured onto conventional media and onto steno medium agar (sma). all isolates confirmed as s. maltophilia by biochemical and molecular methods were subjected to antimicrobial suscepti ...201121763218
stevens-johnson syndrome in a juvenile systemic lupus erythematosus patient.stevens-johnson syndrome (sjs) is a severe and rare immune-mediated cutaneous reaction usually induced by drugs or infections. few case reports have demonstrated sjs associated with adult systemic lupus erythematosus (sle), and rarely in juvenile sle (jsle) patients. however, to the best of our knowledge the prevalence of this life-threatening cutaneous disease in the pediatric lupus population has not been studied. therefore, from january 1983 to december 2010, 5508 patients were followed-up at ...201121768172
an unusual cause of continuous ambulatory peritoneal dialysis-associated outpatient peritonitis: stenotrophomonas maltophilia. 201121782283
xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings.the xenopus laevis oocyte has been the workhorse for the investigation of ion transport proteins. these large cells have spawned a multitude of novel techniques that are unfathomable in mammalian cells, yet the fickleness of the oocyte has driven many researchers to use other membrane protein expression systems. here, we show that some colonies of xenopus laevis are infected with three multi-drug-resistant bacteria: pseudomonas fluorescens, pseudomonas putida, and stenotrophomonas maltophilia. o ...201121788613
biodegradation of geosmin in drinking water by novel bacteria isolated from biologically active carbon.three strains of gram-negative bacteria capable of removing geosmin from drinking water were isolated from biologically active carbon and identified to be chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp. based on physio-biochemistry analysis and 16s rrna gene sequence analysis. removal efficiencies of 2 mg/l geosmin in mineral salts medium were 84.0%, 80.2% and 74.4% for chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp., respectively, while removal efficiencies of ...201121790055
emerging gram-negative infections in burn wounds.gram-negative infection remains a major contributor to morbidity, mortality, and cost of care. in the absence of comparative multinational epidemiological studies specific to burn patients, we sought to review literature trends in emerging gram-negative burn wound infections within the past 60 years. mapping trends in these organisms, although in a minority compared with the six "eskape" pathogens currently being targeted by the infectious diseases society of north america, would identify pathog ...201121792068
use of the diversilab repetitive sequence-based polymerase chain reaction system for genotyping stenotrophomonas maltophilia isolates in different wards of one hospital. 201121802174
identification of stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure.aims:ôçé aim of the study is to identify accurately stenotrophomonas maltophilia isolates recovered from environmental and clinical samples. methods and results:ôçé recovery of sten.-ámaltophilia-like isolates from soil samples using the vancomycin, imipenem, amphotericin b (via) selective agar medium enabled distinction of various morphotype colonies. a set of soil and clinical isolates was tested for species identification using different methods. 16s rdna analyses showed the dark green with a ...201121819497
ciprofloxacin in critically ill children.data regarding the use of fluoroquinolones in critically ill children are scarce. we present our experience regarding the use of ciprofloxacin in this specific patient population. we prospectively identified all paediatric patients who received ciprofloxacin treatment in the intensive care unit of the tertiary care p. & a. kyriakou children's hospital during a three year period (2005 to 2008). eighteen paediatric patients (mean age 23 months, 12 females) who received intravenous ciprofloxacin we ...201121823382
Low Frequency of Endemic Patient-to-Patient Transmission of Antibiotic-Resistant Gram-Negative Bacilli in a Pediatric Intensive Care Unit.We sought to determine the frequency of horizontal transmission of antibiotic-resistant gram-negative bacilli (ARGNB) in a pediatric intensive care unit during a nonoutbreak period. Among 5,300 admissions over 39 consecutive months, 13 ARGNB clusters involving 35 children were identified by pulsed-filed gel electrophoresis analysis, which suggests that person-to-person transmission was uncommon.201121828974
arsenic-resistant bacteria solubilized arsenic in the growth media and increased growth of arsenic hyperaccumulator pteris vittata l.the role of arsenic-resistant bacteria (arb) in arsenic solubilization from growth media and growth enhancement of arsenic-hyperaccumulator pteris vittata l. was examined. seven arb (tolerant to 10mm arsenate) were isolated from the p. vittata rhizosphere and identified by 16s rrna sequencing as pseudomonas sp., comamonas sp. and stenotrophomonas sp. during 7-d hydroponic experiments, these bacteria effectively solubilized arsenic from the growth media spiked with insoluble feaso(4) and alaso(4) ...201121840210
antibacterial and anti-biofilm effects of cathelicidin peptides against pathogens isolated from cystic fibrosis patients.six different cathelicidin-derived peptides were compared to tobramycin for antibacterial and anti-biofilm effects against s. aureus, p. aeruginosa, and s. maltophilia strains isolated from cystic fibrosis patients. overall, smap-29, bmap-28, and bmap-27 showed relevant antibacterial activity (mic(50) 4-8μg/ml), and in some cases higher than tobramycin. in contrast, indolicidin, ll-37, and bac7(1-35) showed no significant antimicrobial activity (mic(50)>32μg/ml). killing kinetics experiments sho ...201121849157
chronic stenotrophomonas maltophilia infection and exacerbation outcomes in cystic fibrosis.chronic stenotrophomonas maltophilia infection is a risk factor for pulmonary exacerbation in cystic fibrosis (cf) but its impact on subsequent clinical outcomes is unknown. the aim of this study was to determine the effect of chronic s. maltophilia infection and associated antimicrobial therapy on the recovery of forced expiratory lung volume in 1s (fev(1)) following pulmonary exacerbation.201121849265
Contemporary unconventional clinical use of co-trimoxazole.Clin Microbiol Infect 2012; 18: 8-17 ABSTRACT: In the late 1960s, the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) was introduced into clinical practice and used to treat many infectious diseases, such as urinary tract infections, respiratory infections, sexually transmitted diseases, Gram-negative sepsis, enteric infections and typhoid fever. Subsequently, co-trimoxazole was reported to be effective against numerous bacterial, fungal and protozoal pathogens, including Noca ...201221851485
screening for novel quorum-sensing inhibitors to interfere with the formation of pseudomonas aeruginosa biofilm.the objective of this study was to screen for novel quorum-sensing inhibitors (qsis) from traditional chinese medicines (tcms) that inhibit bacterial biofilm formation. six of 46 active components found in tcms were identified as putative qsis based on molecular docking studies. of these, three compounds inhibited biofilm formation by pseudomonas aeruginosa and stenotrophomonas maltophilia at a concentration of 200 µm. a fourth compound (emodin) significantly inhibited biofilm formation at 20 µm ...201121852522
hessian fly-associated bacteria: transmission, essentiality, and composition.plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ...201121858016
Frequency of oral mucositis and microbiological analysis in children with acute lymphoblastic leukemia treated with 0.12% chlorhexidine gluconate.In view of the morbidity potential of oral complications in patients with leukemia, this study evaluated the clinical and microbiological alterations that occur in the oral mucosa of children with acute lymphoblastic leukemia (ALL) undergoing antineoplastic chemotherapy and prophylactic administration of 0.12% chlorhexidine gluconate. The sample consisted of 17 children aged 2 to 12 years that underwent clinical examination of the oral mucosa for the detection of oral lesions. In addition, biolo ...201121861031
effect of surfactants on pah biodegradation by a bacterial consortium and on the dynamics of the bacterial community during the process.the aim of this work was to evaluate the effect of a non-biodegradable (tergitol np-10) and a biodegradable (tween-80) surfactant on growth, degradation rate and microbial dynamics of a polycyclic aromatic hydrocarbon (pahs) degrading consortium (c2pl05) from a petroleum polluted soil, applying cultivable and non cultivable techniques. growth and degradation rate were significantly lower with tergitol np-10 than that with tween-80. toxicity did not show any significant reduction with tergitol np ...201121862321
distribution of nosocomial organisms and their resistance patterns in the intensive care unit of the university hospital of the west indies, kingston, jamaica.to determine the distribution and antibiotic susceptibility patterns of nosocomial pathogens in the intensive care unit (icu) at the university hospital of the west indies (uhwi).200921866600
[distribution of microbes and their hereditary diversity in the rhizosphere of carnation in greenhouse].to investigate the relationship between microbial community and the blight diseases in rhizosphere of carnation for biological control.201121866707
Reducing Sugar-Producing Bacteria from Guts of Tenebrio Molitor Linnaeus (Yellow mealworm) for Lignocellulosic Waste Minimization.The guts of Tenebrio Molitor Linnaeus (yellow mealworm) were used as inocula to isolate reducing sugar-producing bacteria during bioconversion of lignocellulose to reducing sugars in this study. Three carbon sources, i.e., carboxymethyl cellulose (CMC), filter paper (FP), and lignocellulosic waste (LIG), were specifically selected; and two types of culturing media (M1 and M2) were used. After 6 months of sequential cultivation, lignocellulose (i.e., polysaccharides) degradation of enrichments M1 ...201121869568
Characteristics of microbial aerosols released from chicken and swine feces.Bioaerosols generated during livestock and poultry production are significant occupational hazards. This study investigates the characteristics of bioaerosols released from animal feces. Fresh feces from pigs and chickens were obtained and tested in a controlled-environment facility. Airborne viable (culturable) bacteria and fungi were sampled hourly for 48 hr. The predominant species were identified via polymerase chain reaction analysis. The number of bacterial colonies released from chicken f ...201121874960
characterization in the archaeological excavation site of heterotrophic bacteria and fungi of deteriorated wall painting of herculaneum in italy.microbiological characterization of frescos in four different locations (collegio degli augustali, casa del colonnato tuscanico, casa dello scheletro and casa del gran portale) of excavation sites of herculaneum was carried out. the use of infrared thermography allowed detecting sample points on frescos with greatest moisture not visible to the naked eye, resulting in structural damage. the microclimatic conditions provided perfect habitat for bacteria and fungi, particularly of spore forming an ...201121882662
antibacterial effects of theaflavin and synergy with epicatechin against clinical isolates of acinetobacter baumannii and stenotrophomonas maltophilia.stenotrophomonas maltophilia and acinetobacter baumannii are recognised as important nosocomial pathogens; however, due to their intrinsic resistance to multiple antibiotics, treatment options are limited. polyphenols from black tea have been shown to possess antibacterial action. in this study, the antibacterial effects of various concentrations of theaflavin as well as combinations of theaflavin and epicatechin were determined using the disk diffusion assay. the results showed strong antibacte ...201121885260
Minimal Inhibitory Concentration of Ceftazidime and Co-trimoxazole for Stenotrophomonas Maltophilia using E-test.Stenotrophomonas maltophilia, previously named as Pseudomonas or Xanthomonas maltophilia, is an important nosocomial pathogen201121887057
how could rapid bacterial identification improve the management of septic patients? 201121905779
The Ax21 protein is a cell-cell signal that regulates virulence in the nosocomial pathogen Stenotrophomonas maltophilia.Stenotrophomonas maltophilia encodes proteins related to the Rax proteins of Xanthomonas oryzae, which are required for the synthesis and secretion of the Ax21 protein. Here we show that Ax21 acts as a cell-cell signal to regulate a diverse range of functions, including virulence, in this nosocomial pathogen.201121908663
Modulation of FAD-dependent monooxygenase activity from aromatic compounds-degrading Stenotrophomonas maltophilia strain KB2.The purpose of this study was purification and characterization of phenol monooxygenase from Stenotrophomonas maltophilia strain KB2, enzyme that catabolises phenol and its derivatives through the initial hydroxylation to catechols. The enzyme requires NADH and FAD as a cofactors for activity, catalyses hydroxylation of a wide range of monocyclic phenols, aromatic acids and dihydroxylated derivatives of benzene except for catechol. High activity of this monooxygenase was observed in cell extrac ...201121927719
contribution of resistance-nodulation-division efflux pump operon smeu1-v-w-u2-x to multidrug resistance of stenotrophomonas maltophilia.kj09c, a multidrug-resistant mutant of stenotrophomonas maltophilia kj, was generated by in vitro selection with chloramphenicol. the multidrug-resistant phenotype of kj09c was attributed to overexpression of a resistance nodulation division (rnd)-type efflux system encoded by an operon consisting of five genes: smeu1, smev, smew, smeu2, and smex. proteins encoded by smev, smew, and smex were similar to the membrane fusion protein, rnd transporter, and outer membrane protein, respectively, of kn ...201121930878
Effective cohorting and "superisolation" in a single intensive care unit in response to an outbreak of diverse multi-drug-resistant organisms.Cohorting patients in dedicated hospital wards or wings during infection outbreaks reduces transmission of organisms, yet frequently, this may not be feasible because of inadequate capacity, especially in the intensive care unit (ICU). We hypothesized that cohorting isolation patients in one geographic location in a single ICU and using enhanced isolation procedures ("superisolation") can prevent the further spread of highly multi-drug-resistant organisms (MDRO).201121936667
nonfermenting gram-negative bacilli infections in a tertiary care hospital in kolar, karnataka.aim: nonfermenting gram-negative bacilli (nfgnb), which are saprophytic in nature, have emerged as important healthcare-associated pathogens. they exhibit resistance not only to beta lactam and the other groups of antibiotics, but also to carbapenems. this study was undertaken to identify the nonfermenters isolated from various clinical samples, to assess their clinical significance, to know the type of healthcare-associated infections they caused, and to know their anti-microbial sensitivity pa ...200921938252
Enhancement of antibiotic susceptibility of Stenotrophomonas maltophilia using a polyclonal antibody developed against an ABC multidrug efflux pump.Stenotrophomonas maltophilia is an emerging nosocomial pathogen capable of causing healthcare-associated infections, including pneumonia and bacteremia. Intrinsic resistance in S. maltophilia is exhibited towards many broad-spectrum antibiotics, and treatment recommendations are controversial. One of the major causes of antimicrobial resistance is attributed to a robust array of efflux pumps that extrude drug compounds from the cell. Using checkerboard and growth kinetic assays, we evaluated the ...201121942332
[Community acquired Stenotrophomonas maltophilia soft-tissue infection in a previously healthy patient.] 201121944651
Identification and characterization of a chitinase of Stenotrophomonas maltophilia, a bacterium that is antagonistic towards fungal phytopathogens.A rhizosphere strain of Stenotrophomonas maltophilia strain MUJ that is strongly antagonistic towards fungal phytopathogens secretes to the culture medium a single form of active chitinolytic enzyme belonging to family 18 of glycosyl hydrolases. The chitinase was purified by a two-stage procedure embracing fractionation with ammonium sulfate and affinity chromatography. The molecular mass of the purified enzyme determined by SDS-PAGE was approximately 52kDa. The enzyme demonstrated highest activ ...201121945415
functional characterization of an nadph dependent 2-alkyl-3-ketoalkanoic acid reductase involved in olefin biosynthesis in stenotrophomonas maltophilia.oled is shown to play a key reductive role in the generation of alkenes (olefins) from acyl thioesters in stenotrophomonas maltophilia. the gene coding for oled clusters with three other genes, oleabc, and all appear to be transcribed in the same direction as an operon in various olefin producing bacteria. in this study, a series of substrates varying in chain length and stereochemistry were synthesized and used to elucidate the functional role and substrate specificity of oled. we demonstrated ...201121958090
the disinfecting potential of contact lens soutions used by sultan qaboos university students.this study aimed to determine the disinfecting potential of some contact lens solutions used by some university students in oman.201121969898
molecular cloning of a copper-dependent laccase from the dye-decolorizing strain stenotrophomonas maltophilia aap56.to clone the gene encoding the enzyme with laccase activity expressed by stenotrophomonas maltophilia aap56 and to construct an insertional mutation in that gene to determine its effect on dye decolourization and laccase activity in this strain.201121973274
A case of Stenotrophomonas maltophilia keratitis effectively treated with moxifloxacin.A 70-year-old man with a long history of diabetes mellitus presented to our hospital (Department of Ophthalmology, Sahm Yook Medical Center, Seoul, Korea) complaining of severe ocular pain and visual disturbance in his left eye that had started three days prior to admission. A round 3.7 × 5.0 mm dense central stromal infiltrate with an overlying epithelial defect was noted on slit-lamp examination. Following corneal scrapings and culture, topical 0.5% moxifloxacin and 0.5% tobramycin were admini ...201121976944
extracellular serine proteases from stenotrophomonas maltophilia: screening, isolation and heterologous expression in e. coli.a large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. encapsulation of cells in alginate beads induced protease production. stenotrophomonas maltophilia emerged as best performer under washing conditions. for identification of wash-active proteases, four extracellular serine proteases called stmpr1, stmpr2, stmpr3 and stmpr4 were clon ...201221983234
biodegradation potential of oily sludge by pure and mixed bacterial cultures.the biodegradation capacity of aliphatic and aromatic hydrocarbons of petrochemical oily sludge in liquid medium by a bacterial consortium and five pure bacterial cultures was analyzed. three bacteria isolated from petrochemical oily sludge, identified as stenotrophomonas acidaminiphila, bacillus megaterium and bacillus cibi, and two bacteria isolated from a soil contaminated by petrochemical waste, identified as pseudomonas aeruginosa and bacillus cereus demonstrated efficiency in oily sludge d ...201121993328
In vitro activity of S-(3,4-dichlorobenzyl)isothiourea hydrochloride and novel structurally related compounds against multidrug-resistant bacteria, including Pseudomonas aeruginosa and Burkholderia cepacia complex.The aim of this study was to establish the antimicrobial activities of S-(3,4-dichlorobenzyl)isothiourea hydrochloride (A22) and a series of structurally related compounds against multidrug-resistant (MDR) bacteria. The minimum inhibitory concentrations (MICs) of 21 compounds were determined against 18 strains of pathogenic bacteria in addition to Pseudomonas aeruginosa (n=19) and Burkholderia cepacia complex (BCC) (n=20) isolated from the sputa of cystic fibrosis patients. Selected compounds we ...201221993484
characterization of copper-resistant rhizosphere bacteria from avena sativa and plantago lanceolata for copper bioreduction and biosorption.copper is a toxic heavy metal widely used to microbial control especially in agriculture. consequently, high concentrations of copper residues remain in soils selecting copper-resistant organisms. in vineyards, copper is routinely used for fungi control. this work was undertaken to study copper resistance by rhizosphere microorganisms from two plants (avena sativa l. and plantago lanceolata l.) common in vineyard soils. eleven rhizosphere microorganisms were isolated, and four displayed high res ...201122002857
microbial biofilms on the sandstone monuments of the angkor wat complex, cambodia.discoloring biofilms from cambodian temples angkor wat, preah khan, and the bayon and west prasat in angkor thom contained a microbial community dominated by coccoid cyanobacteria. molecular analysis identified chroococcidiopsis as major colonizer, but low similarity values (<95%) suggested a similar genus or species not present in the databases. in only two of the six sites sampled were filamentous cyanobacteria, microcoleus, leptolyngbya, and scytonema, found; the first two detected by sequenc ...201122006074
Diversity and assessment of potential risk factors of Gram-negative isolates associated with French cheeses.The goal of this study was to identify at the species level a large collection of Gram-negative dairy bacteria isolated from milks or semi-hard and soft, smear-ripened cheeses (cheese core or surface samples) from different regions of France. The isolates were then assessed for two risk factors, antibiotic resistance and volatile and non-volatile biogenic amine production in vitro. In total, 173 Gram-negative isolates were identified by rrs and/or rpoB gene sequencing. A large biodiversity was o ...201222029922
health care-associated pneumonia (hcap): empiric antibiotics targeting methicillin-resistant staphylococcus aureus (mrsa) and pseudomonas aeruginosa predict optimal outcome.inappropriate initial antimicrobial therapy (iiat) has been associated with decreased survival in patients with health care-associated pneumonia (hcap). we performed a study to determine whether empiric hcap antibiotic regimens targeting methicillin-resistant staphylococcus aureus (mrsa) and pseudomonas aeruginosa are associated with greater appropriate therapy. a retrospective cohort study of culture-positive hcap over 6 years (january 2003-december 2008) was performed at barnes-jewish hospital ...201122033455
dna profiling of stenotrophomonas maltophilia by pcr targeted to its species-specific repetitive palindromic sequences.the aim of this study was to develop a simple protocol for a pcr-based fingerprinting of stenotrophomonas maltophilia (smreppcr) that utilizes primers complementary to repetitive extragenic palindromic elements (reps) of this micro-organism.201222044359
characterization of phragmites cummunis rhizosphere bacterial communities and metabolic products during the two stage sequential treatment of post methanated distillery effluent by bacteria and wetland plants.this study deals with the characterization of rhizosphere bacterial communities and metabolic products produced during the two stage sequential treatment of post methanated distillery effluent by bacteria and constructed wetland plants. results showed that bacterial treatment followed by wetland plants (phragmites cummunis) resulted 94.5% and 96.0% reduction in bod and cod values, respectively. the pcr-rflp analysis showed the presence of stenotrophomonas, enterobacter, pantoea, acinetobacter an ...201222047662
Tsunami lung.We encountered three cases of lung disorders caused by drowning in the recent large tsunami that struck following the Great East Japan Earthquake. All three were females, and two of them were old elderly. All segments of both lungs were involved in all the three patients, necessitating ICU admission and endotracheal intubation and mechanical ventilation. All three died within 3 weeks. In at least two cases, misswallowing of oil was suspected from the features noted at the time of the detection. ...201122057370
stenotrophomonas maltophilia in the respiratory tract of medical intensive care unit patients.the purpose of this study was to investigate characteristics of critically ill patients with stenotrophomonas maltophilia (s. maltophilia) isolated from the respiratory tract, to identify risk factors for s. maltophilia-pneumonia and intensive care unit (icu) mortality and to analyze antibiotic susceptibility of s. maltophilia. this was a retrospective analysis of 64 medical icu patients with s. maltophilia in the respiratory tract. thirty-six patients fulfilled the criteria for diagnosis of pne ...201122057419
adhering ability of stenotrophomonas maltophilia is dependent on growth conditions.the growth conditions are known to influence the bacterial adhesion to different kinds of surfaces. in the present study the adhering ability of stenotrophomonas maltophilia, on growth in nutrient rich media (tryptic soy broth (tsb)) and minimal media (luria bertani (lb)) was checked by viable cell count and spectrophotometric method. tsb grown s. maltophilia showed higher adhesion compared to bacteria grown in lb broth, to both biotic and abiotic surfaces. however, when bacteria were grown in l ...201122073545
cloning and overexpression of ketopantoic acid reductase gene from stenotrophomonas maltophilia and its application to stereospecific production of d: -pantoic acid.ketopantoic acid (kpa) reductase catalyzes the stereospecific reduction of ketopantoic acid to d: -pantoic acid. based on the n-terminal amino acid sequence of kpa reductase from stenotrophomonas maltophilia 845, the kpa reductase gene was cloned from s. maltophilia nbrc14161 and sequenced. this gene contains an open reading frame of 777 bp encoding 258 amino acid residues, and the deduced amino acid sequence showed high similarity to the sdr superfamily proteins. an expression vector, petsmkpr, ...201122083276
stenotrophomonas interspecies differentiation and identification by gyrb sequence analysis.stenotrophomonas species are found commonly in environmental and clinical samples; stenotrophomonas maltophilia is an important opportunistic pathogen of humans. traditional phenotyping protocols, as well as genotyping by 16s rrna gene sequence analysis, do not reliably distinguish the species of stenotrophomonas. sequence analyses of two targeted pcr-amplified regions of the gyrb gene, which encodes the β-subunit of dna gyrase, enabled resolution and identification of these species. most type s ...201122092789
antibiotic resistance and extended-spectrum β-lactamases in isolated bacteria from seawater of algiers beaches (algeria).the aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in algiers. the most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. according to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved f ...201122095134
detection of smqnr quinolone protection protein genes and its prevalence in clinical isolates of stenotrophomonas maltophilia in china.to detect the novel variants of the smqnr family and analyze the prevalence of smqnr genes in clinical isolates of s. maltophilia in china. we collected 442 clinical isolates of s. maltophilia from 9 hospitals of four provinces in china. antimicrobial susceptibility test was performed for these isolates against six commonly used antibiotics (and combinations). the amplified sequences of smqnr genes using pcr were aligned with the known smqnr genes in genbank and smqnr database. the resistance ra ...201122096133
[Observation by transmission electron microscope and identification of endophytic bacteria isolated from Bursaphelenchus xylophilus and B. mucronatus].The pine wood nematode, Bursaphlenchus xylophilus, morphologically similar to B. mucronatus, is the pathogen of pine wilt disease. This study was focused on the endophytic bacteria present in these nematodes.201122097772
Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential.Stenotrophomonas maltophilia is a highly versatile species with useful biotechnological potential but also with pathogenic properties. In light of possible differences in virulence characteristics, knowledge about genomic subgroups is therefore desirable. Two different genotyping methods, rep-PCR fingerprinting and partial gyrB gene sequencing were used to elucidate S. maltophilia intraspecies diversity. Rep-PCR fingerprinting revealed the presence of 12 large subgroups, while gyrB gene sequenci ...201122110692
Bacterial cis-2-unsaturated fatty acids found in the cystic fibrosis airway modulate virulence and persistence of Pseudomonas aeruginosa.There is an increasing appreciation of the polymicrobial nature of many bacterial infections such as those associated with cystic fibrosis (CF) and of the potentially important role for interspecies interactions in influencing both bacterial virulence and response to therapy. Patients with CF are often co-infected with Pseudomonas aeruginosa and other pathogens including Burkholderia cenocepacia and Stenotrophomonas maltophilia. These latter bacteria produce signal molecules of the diffusible si ...201122134647
prognosis of acute respiratory distress syndrome (ards) in neutropenic cancer patients.up today, no study has been specifically designed to identify determinants of death neutropenic cancer patients presenting ards. the aim of this study was to identify early predictive factors of 28-day mortality in these patients. factors associated with 28-day mortality during icu stay were also described.seventy consecutive cancer patients with ards and neutropenia were prospectively analysed over a 6-year period.mortality at 28-day was 63%. factors independently associated with good prognosis ...201122135281
a multicenter surveillance of antimicrobial resistance on stenotrophomonas maltophilia in taiwan.background: stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in debilitated hosts. clinical management of s. maltophilia is challenging due to its intrinsic resistance to a variety of antibiotics. this study investigated the trend and prevalence of antimicrobial resistance in s. maltophilia from a nationwide surveillance study in taiwan. methods: s. maltophilia isolates were collected biennially between 1998 and 2008 as part of the taiwan surveillance of antimicrob ...201122154599
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