Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
aldehyde dehydrogenase (aldh) superfamily in plants: gene nomenclature and comparative genomics.in recent years, there has been a significant increase in the number of completely sequenced plant genomes. the comparison of fully sequenced genomes allows for identification of new gene family members, as well as comprehensive analysis of gene family evolution. the aldehyde dehydrogenase (aldh) gene superfamily comprises a group of enzymes involved in the nad(+)- or nadp(+)-dependent conversion of various aldehydes to their corresponding carboxylic acids. aldh enzymes are involved in processin ...201323007552
natural stilbenoids: distribution in the plant kingdom and chemotaxonomic interest in vitaceae.stilbenoids, a family of polyphenols known for the complexity of their structure and for their diverse biological activities, occur with a limited but heterogeneous distribution in the plant kingdom. the most prominent stilbene containing plant family, the vitaceae, represented by the famous wine producing grape vines vitis vinifera l., is one of the richest sources of novel stilbenes currently known, together with other families, such as dipterocarpaceae, gnetaceae and fabaceae. this review foc ...201223014926
large-scale parentage analysis in an extended set of grapevine cultivars (vitis vinifera l.).inheritance of nuclear microsatellite markers (nssr) has been proved to be a powerful tool to verify or uncover the parentage of grapevine cultivars. the aim of the present study was to undertake an extended parentage analysis using a large sample of vitis vinifera cultivars held in the inra "domaine de vassal" grape germplasm repository (france). a dataset of 2,344 unique genotypes (i.e. cultivars without synonyms, clones or mutants) identified using 20 nssr was analysed with famoz software. pa ...201323015217
transcriptomic analysis of grape (vitis vinifera l.) leaves during and after recovery from heat stress.grapes are a major fruit crop around the world. heat stress can significantly reduce grape yield and quality. changes at the molecular level in response to heat stress and subsequent recovery are poorly understood. to elucidate the effect of heat stress and subsequent recovery on expression of genes by grape leaves representing the classic heat stress response and thermotolerance mechanisms, transcript abundance of grape (vitis vinifera l.) leaves was quantified using the affymetrix grape genome ...201223016701
large-scale characterization of promoters from grapevine (vitis spp.) using quantitative anthocyanin and gus assay systems.successful implementation of cisgenic/intragenic/ingenic technology for crop improvement necessitates a better understanding of the function of native promoters for driving desired gene expression in host plant. although the genome of grapevine (vitis vinifera) has been determined, efforts to explore promoter resources for the development of cisgenics are still lacking. particularly, there is a shortage of constitutive promoters for marker and/or target gene expression in this species. in this w ...201223017908
total phenolic contents and free-radical scavenging activities of grape (vitis vinifera l.) and grape products.grape is one of the world's largest fruit crops, with an approximate annual production of 58 million metric tons, and it is well known that the grape skins, seeds and stems, waste products generated during wine and grape juice processing, are rich sources of polyphenols. it contains flavonoids, phenolic acids and stilbenes. in this study, we tried to determine antioxidant properties and phenolic contents of grape and grape products (fresh fruit, seed, dried fruit, molasses, pestil, vinegar) of e ...201323025419
a candidate-gene association study for berry colour and anthocyanin content in vitis vinifera l.anthocyanin content is a trait of major interest in vitis vinifera l. these compounds affect grape and wine quality, and have beneficial effects on human health. a candidate-gene approach was used to identify genetic variants associated with anthocyanin content in grape berries. a total of 445 polymorphisms were identified in 5 genes encoding transcription factors and 10 genes involved in either the biosynthetic pathway or transport of anthocyanins. a total of 124 snps were selected to examine a ...201223029369
transcriptome variation along bud development in grapevine (vitis vinifera l.).vegetative buds provide plants in temperate environments the possibility for growth and reproduction when environmental conditions are favorable. in grapevine, crucial developmental events take place within buds during two growing seasons in consecutive years. the first season, the shoot apical meristem within the bud differentiates all the basic elements of the shoot including flowering transition in lateral primordia and development of inflorescence primordia. these events practically end with ...201223035802
antimicrobial activity and composition profile of grape (vitis vinifera) pomace extracts obtained by supercritical fluids.the possibility of increasing the aggregated value of the huge amount of residues generated by wineries around the world foment studies using the grape pomace - the residue from the wine production, composed by seed, skin and stems - to obtain functional ingredients. nowadays, consumers in general prefer natural and safe products mainly for food and cosmetic fields, where the supercritical fluid extraction is of great importance due to the purity of the extracts provided. therefore, the objectiv ...201323036924
a putative role for tip and pip aquaporins in dynamics of leaf hydraulic and stomatal conductances in grapevine under water stress and re-watering.we examined the role of aquaporins (aqps) in regulating leaf hydraulic conductance (kleaf ) in vitis vinifera l. (cv chardonnay) by studying effects of aqp inhibitors, and aqp gene expression during water stress (ws) and recovery (rec). kleaf was measured after 3 h of petiole perfusion with different solutions and to introduce inhibitors. the addition of 0.1 mm hgcl2 to 15 mm kcl reduced kleaf compared with perfusion in 15 mm kno3 or kcl, and these solutions were used for leaves from control, ws ...201323046275
a core functional region of the rfp1 promoter from chinese wild grapevine is activated by powdery mildew pathogen and heat stress.ring-finger proteins (rfp) function as ubiquitin ligases and play key roles in plant responses to biotic and abiotic stresses. however, little information is available on the regulation of rfp expression. here, we isolate and characterize the rfp promoter sequence from the disease-resistant chinese wild grape vitis pseudoreticulata accession baihe-35-1. promoter-gus fusion assays revealed that defense signaling molecules, powdery mildew infection, and heat stress induce vprfp1 promoter activity. ...201323053541
pedigree reconstruction of the italian grapevine aglianico (vitis vinifera l.) from campania.a total of 41 accessions of aglianico belonging to three different biotypes (taburno, taurasi, and vulture) and 9 accessions of sirica grapes were sampled from diverse areas of campania (italy). all accessions were first genotyped using 21 microsatellite markers (ssr) to evaluate possible homonymies, synonymies, and the genetic structure of each group. a larger dataset was then constructed adding italian and international cultivars. on the basis of results obtained analyzing the first dataset, f ...201323054630
hepatoprotective activity of vitis vinifera root extract against carbon tetrachloride-induced liver damage in rats.the ethanolic extract of the root of vitis vinifera (vitaceae) was evaluated for hepatoprotective activity in rats with liver damage induced by carbon tetrachloride. the extract at an oral dose of 200 mg/kg exhibited a significant protective effect by lowering the serum levels of sgpt, sgot, alkaline phosphatase and total bilirubin. the extract at this dose also increases the level of total protein. these biochemical observations were supplemented by histopathological examination of liver sectio ...201623061290
metnet online: a novel integrated resource for plant systems biology.plants are important as foods, pharmaceuticals, biorenewable chemicals, fuel resources, bioremediation tools and general tools for recombinant technology. the study of plant biological pathways is advanced by easy access to integrated data sources. today, various plant data sources are scattered throughout the web, making it increasingly complicated to build comprehensive datasets.201223066841
thermal thresholds as predictors of seed dormancy release and germination timing: altitude-related risks from climate warming for the wild grapevine vitis vinifera subsp. sylvestris.the importance of thermal thresholds for predicting seed dormancy release and germination timing under the present climate conditions and simulated climate change scenarios was investigated. in particular, vitis vinifera subsp. sylvestris was investigated in four sardinian populations over the full altitudinal range of the species (from approx. 100 to 800 m a.s.l).201223071219
gut microbial metabolism of polyphenols from black tea and red wine/grape juice is source-specific and colon-region dependent.the colonic microbial degradation of a polyphenol-rich black tea extract (bte) and red wine/grape juice extract (rwge) was compared in a five-stage in vitro gastrointestinal model (twinshime). microbial metabolism of bte and rwge polyphenols in the twinshime was studied subsequently in single- and continuous-dose experiments. a combination of liquid or gas chromatography with mass spectrometry (lc-ms or gc-ms) and nmr-based metabolic profiling was used to measure selected parent polyphenols, the ...201223072624
influence of constitutive phenolic compounds on the response of grapevine (vitis vinifera l.) leaves to infection by plasmopara viticola.flavonols and hydroxycinnamic acids are known to contribute to plant resistance against pathogens, but there are few reports on the implication of flavonols in the resistance of grapevine against plasmopara viticola, and none on the involvement of hydroxycinnamic acids. in order to analyze the effect of flavonols on p. viticola infection, variable amounts of flavonols were induced by different light conditions in otherwise phenologically identical leaves. differences in content of leaf hydroxyci ...201323080015
characterization of mitochondrial dicarboxylate/tricarboxylate transporters from grape berries.grape berries (vitis vinifera l fruit) exhibit a double-sigmoid pattern of development that results from two successive periods of vacuolar swelling during which the nature of accumulated solutes changes significantly. throughout the first period, called green or herbaceous stage, berries accumulate high levels of organic acids, mainly malate and tartrate. at the cellular level fruit acidity comprises both metabolism and vacuolar storage. malic acid compartmentation is critical for optimal funct ...201323096487
proanthocyanidin composition and antioxidant potential of the stem winemaking byproducts from 10 different grape varieties (vitis vinifera l.).stem byproducts from 10 different grape (vitis vinifera l.) varieties were evaluated in terms of their total phenolic and total proanthocyanidin contents, flavan-3-ol and proanthocyanidin profiles, and antioxidant capacity measured by abts, cuprac, frap, and orac assays, with a view to the recovery of their natural bioactive compounds. stems from callet, syrah, premsal blanc, parellada, and manto negro varieties yielded the highest total phenolic and total proanthocyanidin contents and showed th ...201223101762
ogataea kanchanaburiensis sp. nov. and ogataea wangdongensis sp. nov., two novel methylotrophic yeast species from phylloplane in thailand.three strains (km03(t), km13 (t) and km15) representing two novel methylotrophic yeast species were isolated from the external surface of plant leaves, which were collected from kanchanaburi province, thailand, by three-consecutive enrichments in methanol broth. strain km03(t) was isolated from phylloplane of a mango tree (mangifera indica) and two strains, km13(t) and km15, were obtained from phylloplane of different wine grapes (vitis vinifera). the sequences of the d1/d2 region of the large s ...201323104074
proteomic analysis of grapevine resistance induced by trichoderma harzianum t39 reveals specific defence pathways activated against downy mildew.downy mildew is caused by the oomycete plasmopara viticola and is one of the most serious diseases of grapevine. the beneficial microorganism trichoderma harzianum t39 (t39) has previously been shown to induce plant-mediated resistance and to reduce the severity of downy mildew in susceptible grapevines. in order to better understand the cellular processes associated with t39-induced resistance, the proteomic and histochemical changes activated by t39 in grapevine were investigated before and 1 ...201223105132
antioxidant capacity and inhibitory effect of grape seed and rosemary extract in marinades on the formation of heterocyclic amines in fried beef patties.the effect of oil-based marinades containing grape seed extract (vitis vinifera l.; 0.2, 0.4, 0.6 and 0.8 g/100g) formulated in a water/oil emulsion or rosemary extract (rosmarinus officinalis; 0.12, 0.2, 0.6, 1.0 and 1.5 g/100g) in oil on the formation of heterocyclic amines (has) in fried beef patties was examined. after application of marinades and frying, four has meiqx (2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline), phip (2-amino-1-methyl-6-phenylimidazo[4,5b]pyridine), norharman, and harm ...201223107689
influence of deficit irrigation and kaolin particle film on grape composition and volatile compounds in merlot grape (vitis vinifera l.).the effect of deficit irrigation and a kaolin-based, foliar reflectant particle film (pf) on grape composition and volatile compounds in merlot grapes was investigated over two growing seasons in semi-arid, south-western idaho. vines were provided with differential amounts of water based on their estimated crop evapotranspiration (et(c)) throughout berry development, and particle film was applied to half of the vines in each irrigation main plot. free and bound volatile compounds in grapes were ...201223107699
specificity of ocimum basilicum geraniol synthase modified by its expression in different heterologous systems.numerous aromatic plant species produce high levels of monoterpenols, using geranyl diphosphate (gpp) as a precursor. sweet basil (ocimum basilicum) geraniol synthase (ges) was used to evaluate the monoterpenol profiles arising from heterologous expressions in various plant models. grapevine (vitis vinifera) calli were transformed using agrobacterium tumefasciens and the plants were regenerated. thale cress (arabidopsis thaliana) was transformed using the floral dip method. tobacco (nicotiana be ...201323108028
conserved noncoding sequences highlight shared components of regulatory networks in dicotyledonous plants.conserved noncoding sequences (cnss) in dna are reliable pointers to regulatory elements controlling gene expression. using a comparative genomics approach with four dicotyledonous plant species (arabidopsis thaliana, papaya [carica papaya], poplar [populus trichocarpa], and grape [vitis vinifera]), we detected hundreds of cnss upstream of arabidopsis genes. distinct positioning, length, and enrichment for transcription factor binding sites suggest these cnss play a functional role in transcript ...201223110901
dragon tis spotter: an arabidopsis-derived predictor of translation initiation sites in plants.in higher eukaryotes, the identification of translation initiation sites (tiss) has been focused on finding these signals in cdna or mrna sequences. using arabidopsis thaliana (a.t.) information, we developed a prediction tool for signals within genomic sequences of plants that correspond to tiss. our tool requires only genome sequence, not expressed sequences. its sensitivity/specificity is for a.t. (90.75%/92.2%), for vitis vinifera (66.8%/94.4%) and for populus trichocarpa (81.6%/94.4%), whic ...201323110968
transcriptional expression of stilbene synthase genes are regulated developmentally and differentially in response to powdery mildew in norton and cabernet sauvignon grapevine.stilbenic compounds are natural phytoalexins that have antimicrobial activities in plant defense against pathogens. stilbene synthase (sts) is the key enzyme that catalyzes the biosynthesis of stilbenic compounds. grapevine genome contains a family of preliminarily annotated 35 sts genes, the regulation of each sts gene needs to be studied to define their roles. in this study, we selected eight sts genes, sts8, sts27/31, sts16/22, sts13/17/23, and applied quantitative polymerase chain reaction ( ...201223116673
complete genome of a novel endornavirus assembled from next-generation sequence data.endornaviruses have large double-stranded rna (dsrna) genomes that carry a single open reading frame (orf). here we report the complete genome of a novel endornavirus, assembled from next-generation sequence data generated from vitis vinifera-extracted dsrna. two different fungal hosts have been identified for this virus, suggesting that horizontal transmission of the virus is possible.201223118465
a simple gc-ms method for the screening of betulinic, corosolic, maslinic, oleanolic and ursolic acid contents in commercial botanicals used as food supplement ingredients.the occurrence of triterpene pentacyclic acids in plants is extensive, but little is known about their availability in commercial extracts. a simple gc-ms method for the simultaneous determination of betulinic, corosolic, maslinic, oleanolic and ursolic acids was developed and applied to 38 different commercial plant extracts sold as ingredients for dietary supplements. a suitable protocol was set up to perform routine control of a diverse array of samples with different botanical, chemical and ...201323122121
lachancea lanzarotensis sp. nov., an ascomycetous yeast isolated from grapes and wine fermentation in lanzarote, canary islands.during the characterization of the microbiota biodiversity associated with grapes and wineries in different bioclimatic conditions of the canary islands (spain), a novel yeast species was isolated from lanzarote, the driest wine-producing region of the archipelago. seven strains isolated from grapes, microvinifications and wineries are described. sequence analysis of the d1/d2 domain of the lsu rdna gene and 5.8s-its regions revealed that the isolates were phylogenetically a member of the genus ...201323125316
induction of trans-resveratrol and extracellular pathogenesis-related proteins in elicited suspension cultured cells of vitis vinifera cv monastrell.suspension-cultured cells of vitis vinifera cv monastrell were used to investigate the effects of methyljasmonate, ethylene and salicylic acid separately or in combination with cyclodextrins on both trans-resveratrol production and the induction of defense responses. the results showed that the addition of methyljasmonate or ethylene to suspension-cultured cells jointly treated with cyclodextrins and salicylic acid provoked a decrease of trans-resveratrol levels suggesting that salicylic acid ha ...201323127362
the relationship between root hydraulics and scion vigour across vitis rootstocks: what role do root aquaporins play?vitis vinifera scions are commonly grafted onto rootstocks of other grape species to influence scion vigour and provide resistance to soil-borne pests and abiotic stress; however, the mechanisms by which rootstocks affect scion physiology remain unknown. this study characterized the hydraulic physiology of vitis rootstocks that vary in vigour classification by investigating aquaporin (vvpip) gene expression, fine-root hydraulic conductivity (lp(r)), % aquaporin contribution to lp(r), scion trans ...201223136166
crystal structure of an indole-3-acetic acid amido synthetase from grapevine involved in auxin homeostasis.auxins are important for plant growth and development, including the control of fruit ripening. conjugation to amino acids by indole-3-acetic acid (iaa)-amido synthetases is an important part of auxin homeostasis. the structure of the auxin-conjugating gretchen hagen3-1 (gh3-1) enzyme from grapevine (vitis vinifera), in complex with an inhibitor (adenosine-5'-[2-(1h-indol-3-yl)ethyl]phosphate), is presented. comparison with a previously published benzoate-conjugating enzyme from arabidopsis thal ...201223136372
mapping genetic loci for tolerance to lime-induced iron deficiency chlorosis in grapevine rootstocks (vitis sp.).iron is essential to plants for chlorophyll formation as well as for the functioning of various iron-containing enzymes. iron deficiency chlorosis is a wide-spread disorder of plants, in particular, of those growing on calcareous soils. among the different ways to control iron deficiency problems for crops, plant material and especially rootstock breeding is a suitable and reliable method, especially for fruit trees and grapes. the aim of the experiment was to characterize the genetic basis of g ...201323139142
impact of cover crops in vineyard on the aroma compounds of vitis vinifera l. cv cabernet sauvignon wine.this study compared the influence of different cover crops with clean tillage on wine aroma compounds of 5-year-old cabernet sauvignon vines. white clover, alfalfa, and tall fescue were used in the vineyard and compared with clean tillage. aroma compounds of wine were analysed by solid-phase microextraction gas chromatography-mass spectrometry (spme-gc/ms). forty-seven volatile compounds were identified and quantified. wines made from grapes grown with various cover crops had higher levels of ar ...201123140695
identification of isomers of resveratrol dimer and their analogues from wine grapes by hplc/ms(n) and hplc/dad-uv.a facile method based on hplc/(-)esi-ms(n) is established for the analysis of seven isomers of resveratrol dimers and three of their analogues in xinjiang wine grapes. the structures of these compounds are positively or tentatively determined. among them, three are tentatively identified as new compounds. ms(n) experiments on the [m-h](-) ions provide abundant structural information, especially regarding the relative abundance of the key product ions, m/z 333 and 369 (385 in compound 3), which c ...201123140727
phenolics and their antifungal role in grapevine wood decay: focus on the botryosphaeriaceae family.the interaction between vitis vinifera and trunk disease fungi requires better understanding. we studied the role of phenolics as possible plant defense compounds in this context. the impact of 24 grapevine phenolic compounds was determined on 6 major wood decay fungi by an in vitro agar plate assay. hydroxystilbenoids, especially oligomers such as miyabenol c, isohopeaphenol, and vitisin a and b, greatly reduced the growth of the fungi, except that of phaeoacremonium aleophilum . a detailed inv ...201223145924
early season host plants of apolygus lucorum (heteroptera: miridae) in northern china.apolygus lucorum (meyer-dür) (heteroptera: miridae) has become a severe pest of cotton and many other crops in northern china as a result of the widespread adoption of bacillus thuringiensis (berliner) cotton, with a corresponding reduction of broad-spectrum insecticide application in cotton fields. from the middle of april to middle june, a. lucorum feeds and develops on other host plants before dispersing to cotton fields. effective suppression of a. lucorum populations before they enter cotto ...201223156156
cytotoxic effect of natural trans-resveratrol obtained from elicited vitis vinifera cell cultures on three cancer cell lines.trans-resveratrol (trans-r) has been reported to be a potential cancer chemopreventive agent. although its cytotoxic activity against different cancer cell lines has been tested, its effect on human acute leukemia cell lines has scarcely been investigated, and only a few in vitro studies were performed using human breast epithelial cell lines. due to its potential value for human health, demand for trans-r has rapidly increased, and new biotechnological strategies to obtain it from natural edibl ...201223161277
behavior of 3-isobutyl-2-hydroxypyrazine (ibhp), a key intermediate in 3-isobutyl-2-methoxypyrazine (ibmp) metabolism, in ripening wine grapes.3-isobutyl-2-hydroxypyrazine (ibhp) is thought to be a key intermediate in both the biosynthesis and degradation of the herbaceous smelling 3-isobutyl-2-methoxypyrazine (ibmp), but its behavior during the growing season is not well understood. first, an improved method for ibhp quantification was developed. a deuterated ibhp standard was added to samples prior to isolation by mixed-mode cation exchange solid phase extraction. extracts were silylated prior to quantification by gc-ms. a limit of d ...201223163527
uv-b impairs growth and gas exchange in grapevines grown in high altitude.we previously demonstrated that solar ultraviolet-b (uv-b) radiation levels in high altitude vineyards improve berry quality in vitis vinifera cv. malbec, but also reduce berry size and yield, possibly as a consequence of increased oxidative damage and growth reductions (lower photosynthesis). the defense mechanisms toward uv-b signal and/or evoked damage promote production of antioxidant secondary metabolites instead of primary metabolites. purportedly, the uv-b effects will depend on tissues d ...201323167433
downy mildew resistance induced by trichoderma harzianum t39 in susceptible grapevines partially mimics transcriptional changes of resistant genotypes.downy mildew, caused by plasmopara viticola, is one of the most severe diseases of grapevine and is commonly controlled by fungicide treatments. the beneficial microorganism trichoderma harzianum t39 (t39) can induce resistance to downy mildew, although the molecular events associated with this process have not yet been elucidated in grapevine. a next generation rna sequencing (rna-seq) approach was used to study global transcriptional changes associated with resistance induced by t39 in vitis v ...201223173562
[effects of acetochlor on the photosynthetic and fluorescence characteristics and chloroplast structure of grape leaves].taking one-year old sand-cultured kyoho (vitis vinifera x vĩtis labrusca) grapevines as test materials, this paper studied the effects of spraying acetochlor on the grape leaves photosynthetic and fluorescence characteristics and chloroplast ultra-structure. on the 13th day after spraying acetochlor, the net photosynthesis rate and stomatal conductance of upper-node leaves decreased significantly, so as the maximal photochemistry of psii and the quantum yield of psii electron transport. the rela ...201223189697
temperature-dependent kinetics of grape seed phenolic compounds extraction: experiment and model.the kinetics of a batch solid-liquid extraction of total phenolic compounds (pc) from milled grape seed (vitis vinifera l. cv. "frankovka") using 50% ethanol at different extraction temperatures (25-80°c) was studied. the maximum yield of pc was 0.13 kg(gae)/kg(db) after 200 min of extraction in agitated vessel at 80°c. a new model based on the assumptions of a first order kinetics mechanism for the solid-liquid extraction and a linear equilibrium at the solid-liquid interface was developed. the ...201323194505
the effect of myo-inositol on the growth of callus tissue in vitis.on m2-medium, which is a revised linsmaier and skoogmedium, callus tissue of vitis vinifera cv. müller-thurgau shows normal growth not dependent upon myo-inositol. callus cells of this cultivar may therefore be able to synthesize myo-inositol. callus cells of the cv. aris require myo-inositol and show increasing growth up to an optimum of 250 mg 1(-1). further increase of the myo-inositol concentration resulted in decreasing growth. growth promotion may be due to a higher cell division rate and ...198423195051
impact of clonal variability in vitis vinifera cabernet franc on grape composition, wine quality, leaf blade stilbene content, and downy mildew resistance.in this study, 10 clones of vitis vinifera cabernet franc (not yet commercial) have been phenotyped on precocity, grape composition, and assessment of wine quality made by microvinification in 2008-2010. additionally, two original criteria have been considered: concentration of 3-isobutyl-2-methoxypyrazine (ibmp) in grapes and wines (the green bell pepper flavor) and resistance of grapevines to downy mildew ( plasmopara viticola ) by stilbene quantification upon infection. precocity of veraison ...201323205623
antioxidative responses in vitis vinifera infected by grapevine fanleaf virus.the antioxidative response of grapevine leaves (vitis vinifera cv. trebbiano) affected by the presence of grapevine fanleaf virus was studied during the summer of 2010 at three different harvest times (july 1st and 26th, and august 30th). at the first and second harvest, infected leaves showed increases in the concentration of superoxide radical and hydrogen peroxide, the latter increasing for enhanced activity of superoxide dismutase. in contrast, at the last harvest time, increases in the asco ...201323206931
early signaling events in grapevine cells elicited with cyclodextrins and methyl jasmonate.the use of cyclic oligosaccharides like cyclodextrins (cds), alone or combined with methyl jasmonate (mj), as elicitors has proved very effective in stimulating the production of trans-resveratrol (trans-r) in vitis vinifera suspension-cultured cells (scc). since elicitors can be used to increase trans-r production, understanding the molecular mechanisms involved would improve the management of grapevine cells as factories of this compound. the results obtained in this study provide evidence for ...201323208304
chemopreventive properties of raisins originating from greece in colon cancer cells.colorectal cancer is one of the major causes of cancer-related mortality in humans in both developed and developing countries. dietary patterns influence the risk of colon cancer development, while plant-derived foods have gained great interest, due to the high content of antioxidants. corinthian raisins (currants, cr) and sultanas (s) (vitis vinifera l., vitaceae) are dried vine fruits produced in greece with many culinary uses in both the mediterranean and the western nutrition. in the present ...201323211994
the reflectivity in the s-band and the broadband ultrasonic spectroscopy as new tools for the study of water relations in vitis vinifera l.the large water requirements of vitis vinifera l. together with an increase in temperature and drought events imply the need for irrigation in the driest areas of its distribution range. generous watering may reduce grape quality so irrigation should be precisely regulated through the development of new methods of accurate irrigation scheduling based on plant 'stress sensing'. two new methods, the reflectivity in the s-band and the broadband ultrasonic spectroscopy, can be used as non-invasive a ...201323216204
the first genetic map of the american cranberry: exploration of synteny conservation and quantitative trait loci.the first genetic map of cranberry (vaccinium macrocarpon) has been constructed, comprising 14 linkage groups totaling 879.9 cm with an estimated coverage of 82.2 %. this map, based on four mapping populations segregating for field fruit-rot resistance, contains 136 distinct loci. mapped markers include blueberry-derived simple sequence repeat (ssr) and cranberry-derived sequence-characterized amplified region markers previously used for fingerprinting cranberry cultivars. in addition, ssr marke ...201323224333
transcriptome analysis at four developmental stages of grape berry (vitis vinifera cv. shiraz) provides insights into regulated and coordinated gene expression.vitis vinifera berry development is characterised by an initial phase where the fruit is small, hard and acidic, followed by a lag phase known as veraison. in the final phase, berries become larger, softer and sweeter and accumulate an array of organoleptic compounds. since the physiological and biochemical makeup of grape berries at harvest has a profound impact on the characteristics of wine, there is great interest in characterising the molecular and biophysical changes that occur from flower ...201223227855
dietary grapes (vitis vinifera) feeding attenuates ethanol-induced oxidative stress in blood and modulates immune functions in mice.ethanol metabolism is known to induce overwhelming production of reactive oxygen species (ros) and also to cause associated immune dysfunction. several interventional agents of plant origin, in particular fruits and vegetables have been used to counteract these alterations induced by ethanol. in this study, we investigated the efficacy of dietary feeding of skin and flesh of grapes (vitis vinifera l.) on the alterations in immune and vascular functions in mice with liver abnormalities induced by ...201223259325
an acyl-coa-binding protein from grape that is induced through er stress confers morphological changes and disease resistance in arabidopsis.we here report characterization of a grape (vitis vinifera) acyl-coa-binding protein (vvacbp). expression of vvacbp was detected in grape leaves exposed to tunicamycin-induced endoplasmic reticulum (er) stress as well as cold and heat shock treatments. in tendrils and peduncles, however, high-temperature treatment induced bip (luminal binding protein) expression, a marker of er stress in berry skin, but not vvacbp expression. we hypothesize that vvacbp may be sorted to the periphery of plant cel ...201323261264
purification and structural characterisation of lipid transfer protein from red wine and grapes.lipid transfer proteins (ltp) play a major role in plant defence and are of particular interest due to their known ability to cause allergic reactions. these proteins are expressed in grapes and also remain detectable after vinification, especially in red wine. however, it remains unknown whether the protein undergoes any changes during the vinification process. here, we present a purification method for ltps from dornfelder grapes and wine. by liquid-chromatography-mass spectroscopy (lc-ms/ms) ...201323265486
wine grape pomace as antioxidant dietary fibre for enhancing nutritional value and improving storability of yogurt and salad dressing.wine grape pomace (wgp) as a source of antioxidant dietary fibre (adf) was fortified in yogurt (y), italian (i) and thousand island (t) salad dressings. during the 3 weeks of storage at 4 °c, viscosity and ph of wgp-y increased and decreased, respectively, but syneresis and lactic acid percentage of wgp-y and ph of wgp-i and wgp-t were stable. adding wgp resulted in 35-65% reduction of peroxide values in all samples. dried whole pomace powder (wp) fortified products had dietary fibre content of ...201323265499
whole genome identification and analysis of fk506-binding protein family genes in grapevine (vitis vinifera l.).in plant and animal species fk506-binding protein (fkbp) family genes are important conserved genes and it is defined as the receptors of fk506 and rapamycin, where they work as ppiase and protein folding chaperones. fkbp have been isolated from arabidopsis thaliana, oryza sativa, and zea mays. in grape, twenty-three genes containing the fk506-binding domain (fkbp_c) were first time identified by hmmer and blast research, they were classified into three groups and 17 out of the 23 genes were loc ...201323269629
stilbenoid profiles of canes from vitis and muscadinia species.we present stilbenoid profiles of canes from 16 grapevines. fifteen stilbenoids were obtained through isolation and structure identification using ms, nmr, and [α](d) or as commercial standards. an hplc-uv method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of vitis amurensis, vitis arizonica, vitis berlandieri, vitis betulifolia, vitis cinerea, vitis × champini, vitis × doaniana, vitis labrusca, vitis candicans (syn. vitis mustangensis), vi ...201323270496
effect of rain-shelter cultivation of vitis vinifera cv. cabernet gernischet on the phenolic profile of berry skins and the incidence of grape diseases.rain-shelter cultivation is an effective cultural method to prevent rainfall damage during grape harvest and widely applied in the chinese rainy regions. in this study we investigated the effect of rain-shelter cultivation on grape diseases and phenolic composition in the skins of vitis vinifera cv. cabernet gernischet grape berries through the comparison with open-field cultivation at two vintages (2010 and 2011). the results showed that rain-shelter cultivation reduced the incidence of grape d ...201223271472
identification of muscadine wine sulfur volatiles: pectinase versus skin-contact maceration.muscadine grapes ( vitis rotundifolia ) are widely grown in the southern united states, as the more common vitis vinifera cannot be cultivated due to pierce's disease. there is interest to determine if certain cultivars can be used for good-quality wine production. this study compared the effect of pectolytic enzyme pretreatment with conventional skin-contact fermentation on muscadine (noble, vitis rotundifolia ) wine major volatiles, aroma active volatiles, and volatile sulfur compounds (vscs). ...201323289372
vascular occlusions in grapevines with pierce's disease make disease symptom development worse.vascular occlusions are common structural modifications made by many plant species in response to pathogen infection. however, the functional role(s) of occlusions in host plant disease resistance/susceptibility remains controversial. this study focuses on vascular occlusions that form in stem secondary xylem of grapevines (vitis vinifera) infected with pierce's disease (pd) and the impact of occlusions on the hosts' water transport and the systemic spread of the causal bacterium xylella fastidi ...201323292789
nocturnal and daytime stomatal conductance respond to root-zone temperature in 'shiraz' grapevines.daytime root-zone temperature may be a significant factor regulating water flux through plants. water flux can also occur during the night but nocturnal stomatal response to environmental drivers such as root-zone temperature remains largely unknown.201323293018
crystallization and preliminary x-ray crystallographic analysis of udp-glucuronic acid:flavonol-3-o-glucuronosyltransferase (vvgt5) from the grapevine vitis vinifera.grapevine (vitis vinifera) glycosyltransferase 5 (vvgt5) is a udp-glucuronic acid:flavonol-3-o-glucuronosyltransferase that catalyses the 3-o-specific glucuronosylation of flavonols using udp-glucuronic acid as a sugar donor to produce flavonol 3-o-glucosides, which are important bioactive phytochemicals. recombinant vvgt5 expressed in escherichia coli cells was purified and crystallized by the sitting-drop vapour-diffusion method. a full set of x-ray diffraction data was collected to 2.2 å brag ...201323295490
complete genome sequence analysis of an american isolate of grapevine virus e.the complete genome sequence of grapevine virus e (gve) collected from a red-berried wine grape cultivar (cabernet sauvignon) in washington state was determined. the 7,568 nucleotide long genome of gve is more similar in sequence identity with a gve isolate from a wine grape cv. shiraz from south africa when compared with an isolate from "aki queen" grape from japan. like gve isolates from south africa and japan, the washington isolate encodes five open reading frames (orfs) and the overall geno ...201323296875
comparison of ultrasound-assisted extraction with conventional extraction methods of oil and polyphenols from grape (vitis vinifera l.) seeds.ultrasound-assisted extraction (us) carried out at 20 khz, 150 w for 30 min gave grape seed oil yield (14% w/w) similar to soxhlet extraction (s) for 6 h. no significant differences for the major fatty acids was observed in oils extracted by s and us at 150 w. instead, k232 and k268 of us- oils resulted lower than s-oil. from grape seeds differently defatted (s and us), polyphenols and their fractions were extracted by maceration for 12 h and by ultrasound-assisted extraction for 15 min. sonicat ...201323305938
generic and sequence-variant specific molecular assays for the detection of the highly variable grapevine leafroll-associated virus 3.grapevine leafroll-associated virus 3 (glrav-3) is an economically important virus, which is found in all grapevine growing regions worldwide. its accurate detection in nursery and field samples is of high importance for certification schemes and disease management programmes. to reduce false negatives that can be caused by sequence variability, a new universal primer pair was designed against a divergent sequence data set, targeting the open reading frame 4 (heat shock protein 70 homologue gene ...201323313884
the grape berry-specific basic helix-loop-helix transcription factor vvceb1 affects cell size.the development of fleshy fruits involves complex physiological and biochemical changes. after fertilization, fruit growth usually begins with cell division, continues with both cell division and expansion, allowing fruit set to occur, and ends with cell expansion only. in spite of the economical importance of grapevine, the molecular mechanisms controlling berry growth are not fully understood. the present work identified and characterized vitis vinifera cell elongation bhlh protein (vvceb1), a ...201323314819
plant food supplements with anti-inflammatory properties: a systematic review (i).plant food supplements (pfs) receive great acceptance by european consumers. however, quality and efficacy of these products remain a question of concern. the aim of this systematic review is to summarize and critically evaluate the evidence for or against the efficacy of pfs for coping inflammatory conditions by considering epidemiological and human intervention studies. the review, which consists of two parts, considers olea europea l., camellia sinensis l., vitis vinifera l., and matricaria r ...201323320910
using an automatic resistivity profiler soil sensor on-the-go in precision viticulture.spatial information on vineyard soil properties can be useful in precision viticulture. in this paper a combination of high resolution soil spatial information of soil electrical resistivity (er) and ancillary topographic attributes, such as elevation and slope, were integrated to assess the spatial variability patterns of vegetative growth and yield of a commercial vineyard (vitis vinifera l. cv. tempranillo) located in the wine-producing region of la rioja, spain. high resolution continuous ge ...201323325171
de novo transcriptome characterization of vitis vinifera cv. corvina unveils varietal diversity.plants such as grapevine (vitis spp.) display significant inter-cultivar genetic and phenotypic variation. the genetic components underlying phenotypic diversity in grapevine must be understood in order to disentangle genetic and environmental factors.201323331995
'candidatus phytoplasma solani', a novel taxon associated with stolbur- and bois noir-related diseases of plants.phytoplasmas classified in group 16srxii infect a wide range of plants and are transmitted by polyphagous planthoppers of the family cixiidae. based on 16s rrna gene sequence identity and biological properties, group 16srxii encompasses several species, including 'candidatus phytoplasma australiense', 'candidatus phytoplasma japonicum' and 'candidatus phytoplasma fragariae'. other group 16srxii phytoplasma strains are associated with stolbur disease in wild and cultivated herbaceous and woody pl ...201323334879
xylem vessel relays contribute to radial connectivity in grapevine stems (vitis vinifera and v. arizonica; vitaceae).xylem network connections play an important role in water and nutrient transport in plants, but also facilitate the spread of air embolisms and xylem-dwelling pathogens. this study describes the structure and function of vessel relays found in grapevine xylem that form radial and tangential connections between spatially discrete vessels.201323345417
structural, kinetic and computational investigation of vitis vinifera dhdps reveals new insight into the mechanism of lysine-mediated allosteric inhibition.lysine is one of the most limiting amino acids in plants and its biosynthesis is carefully regulated through inhibition of the first committed step in the pathway catalyzed by dihydrodipicolinate synthase (dhdps). this is mediated via a feedback mechanism involving the binding of lysine to the allosteric cleft of dhdps. however, the precise allosteric mechanism is yet to be defined. we present a thorough enzyme kinetic and thermodynamic analysis of lysine inhibition of dhdps from the common grap ...201323354837
molecular characterization of dna repair protein ku70 from vitis vinifera and its purification from transgenic tobacco.the dna double strand break repair in plants is preferentially by non homologous end joining (nhej) pathway. a key protein of nhej pathway is ku70. we have identified ku70 homolog (vvku70) from grapevine genome database. in this report we characterize a ku70 homologue from vitis vinifera cv. mango. the vvku70 expression was found to increase strongly in response to gamma radiation. the transcript level of vvku70 was found to increase up to 36 h in gamma irradiated shoots of grapevine. the expres ...201323361869
metabolic profiling reveals coordinated switches in primary carbohydrate metabolism in grape berry (vitis vinifera l.), a non-climacteric fleshy fruit.changes in carbohydrate metabolism during grape berry development play a central role in shaping the final composition of the fruit. the present work aimed to identify metabolic switches during grape development and to provide insights into the timing of developmental regulation of carbohydrate metabolism. metabolites from central carbon metabolism were measured using high-pressure anion-exchange chromatography coupled to tandem mass spectrometry and enzymatic assays during the development of gr ...201323364938
assessment of polyphenolic content, antioxidant activity, protection against ros-induced dna damage and anticancer activity of vitis vinifera stem extracts.grape extracts and wine have been studied widely due to their beneficial effects on human health. however, there are only few studies from grape stems extracts. therefore, the main objective of the present study was the assessment in stem extracts from greek vitis vinifera varieties of the total polyphenolic content (tpc), the identification of the polyphenols present in them, and the evaluation of their antioxidant activity, protection against ros-induced dna damage and inhibition of liver (hep ...201323380202
[in vitro effect of ankaferd blood stopper®, a plant extract against mycobacterium tuberculosis isolates].treatment of drug-resistant mycobacterium tuberculosis infections requires combination of anti-tuberculosis drugs which have several toxic side effects. thus there is a need for safer and effective new drugs. ankaferd blood stopper® (abs), which is a mixture of plant extracts prepared from alpinia officinarum, glycyrrhiza glabra, thymus vulgaris, urtica dioica and vitis vinifera, has homeostatic and antibacterial effects. standard solutions of abs are already being used topically for post-trauma ...201323390904
large-scale proteomic analysis of the grapevine leaf apoplastic fluid reveals mainly stress-related proteins and cell wall modifying enzymes.the extracellular space or apoplast forms a path through the whole plant and acts as an interface with the environment. the apoplast is composed of plant cell wall and space within which apoplastic fluid provides a means of delivering molecules and facilitates intercellular communications. however, the apoplastic fluid extraction from in planta systems remains challenging and this is particularly true for grapevine (vitis vinifera l.), a worldwide-cultivated fruit plant. large-scale proteomic an ...201323391302
genetic structure in cultivated grapevines is linked to geography and human selection.grapevine (vitis vinifera subsp. vinifera) is one of the most important and ancient horticultural plants in the world. domesticated about 8-10,000 years ago in the eurasian region, grapevine evolved from its wild relative (v. vinifera subsp. sylvestris) into very diverse and heterozygous cultivated forms. in this work we study grapevine genetic structure in a large sample of cultivated varieties, to interpret the wide diversity at morphological and molecular levels and link it to cultivars utili ...201323394135
a simple method for comparing fungal biomass in infected plant tissues.plant phenotypes resistant and susceptible to fungal pathogens are usually scored using qualitative, subjective methods that are based upon disease symptoms or by an estimation of the amount of visible fungal growth. given that plant resistance genes often confer partial resistance to fungal pathogens, a simple, sensitive, nonsubjective quantitative method for measuring pathogen growth would be highly advantageous. this report describes an in planta quantitative assay for fungal biomass based up ...201323405866
relationships between harvest time and wine composition in vitis vinifera l. cv. cabernet sauvignon 1. grape and wine chemistry.the study aimed to quantify the effects of grape maturity on wine alcohol, phenolics, flavour compounds and polysaccharides in vitis vinifera l. cv cabernet sauvignon. grapes were harvested at juice soluble solids from 20 to 26 °brix which corresponded to a range of wine ethanol concentrations between 12% and 15.5%. grape anthocyanin and skin tannin concentration increased as ripening progressed, while seed tannin declined. in the corresponding wines, monomeric anthocyanin and wine tannin concen ...201323411300
evolution of flavonoids in mouratón berries taken from both bunch halves.galicia (n.w. spain) is a spanish region with several old-traditional winegrowing areas. there are autochthonous grapevine varieties, such as vitis vinifera l. cv. mouratón, considered a biodiversity resource in viticulture and an opportunity for galician sustainable wine production. therefore, it is necessary to assess the potential of traditional cultivars to produce quality red wines. in this work, anthocyanin and flavonol evolution was followed in red berries from v. vinifera l. cv. mouratón ...201323411319
relative susceptibility of vitis vinifera cultivars to vector-borne xylella fastidiosa through time.understanding the interactions between pathogen, crop and vector are necessary for the development of disease control practices of vector-borne pathogens. for instance, resistant plant genotypes can help constrain disease symptoms due to infections and limit pathogen spread by vectors. on the other hand, genotypes susceptible to infection may increase pathogen spread owing to their greater pathogen quantity, regardless of their symptom status. in this study, we evaluated under greenhouse conditi ...201323424629
water deficit modulates the response of vitis vinifera to the pierce's disease pathogen xylella fastidiosa.pierce's disease, caused by the bacterium xylella fastidiosa, is one of the most devastating diseases of cultivated grape, currently restricted to the americas. to test the long-standing hypothesis that pierce's disease results from pathogen-induced drought stress, we used the affymetrix vitis genechip to compare the transcriptional response of vitis vinifera to xylella infection, water deficit, or a combination of the two stresses. the results reveal a redirection of gene transcription involvin ...201323425100
the relationships between consumer liking, sensory and chemical attributes of vitis vinifera l. cv. pinotage wines elaborated with different oenococcus oeni starter cultures.malolactic fermentation (mlf) mediated by lactic acid bacteria (lab) has been shown to modulate chemical and sensory attributes of wine. this study investigated the relation between consumer liking, chemical and sensory attributes of vitis vinifera l. cv. pinotage wines that were made over two vintages by four different lactic acid oenococcus oeni starter cultures as well as a control treatment where mlf was prevented.201323427009
selective defoliation affects plant growth, fruit transcriptional ripening program and flavonoid metabolism in grapevine.the selective removal of grapevine leaves around berry clusters can improve the quality of ripening fruits by influencing parameters such as the berry sugar and anthocyanin content at harvest. the outcome depends strongly on the timing of defoliation, which influences the source-sink balance and the modified microclimate surrounding the berries. we removed the basal leaves from vitis vinifera l. cv sangiovese shoots at the pre-bloom and veraison stages, and investigated responses such as shoot g ...201323433030
in vivo visualizations of drought-induced embolism spread in vitis vinifera.long-distance water transport through plant xylem is vulnerable to hydraulic dysfunction during periods of increased tension on the xylem sap, often coinciding with drought. while the effects of local and systemic embolism on plant water transport and physiology are well documented, the spatial patterns of embolism formation and spread are not well understood. using a recently developed nondestructive diagnostic imaging tool, high-resolution x-ray computed tomography, we documented the dynamics ...201323463781
protective potential of black grapes against lead induced oxidative stress in rats.from time immemorial vitis vinifera (black grapes) have been used both for medicinal and nourishment purposes. the aim of this study is to investigate the protective effect of black grapes against lead nitrate induced oxidative stress. exposure to lead significantly increased malondialdehyde levels with a significant decrease in superoxide dismutase and catalase activities, and the concentration of gsh in the liver and kidneys of rats. significantly increased levels of ast, alt, alp, bun and ser ...201323467113
isolation of resveratrol from vitis viniferae caulis and its potent inhibition of human tyrosinase.tyrosinase (tyr) catalyzes rate-limiting reactions of cellular melanin synthesis, and its inhibitors are of commercial interest as potential skin whitening agents. however, the limited availability of human tyr makes the screening of tyr inhibitors difficult. to overcome this hurdle, we transformed nonmelanocytic human embryonic kidney (hek) 293 cells to express human tyr constitutively. using these cells as a source of human tyr, the ethanolic extracts of 52 medicinal plants grown in korea were ...201323476698
expression of grape class iv chitinase in spodoptera frugiperda (sf9) insect cells.most of pathogenesis related (pr) proteins possess complicated structures; hence their active recombinant forms are usually produced in eukaryotic systems. in this study, we employed an insect cell line to express a recombinant form of a previously identified grape pr3 allergen categorised as class iv chitinase.201623481555
validation assay of p3_vvagl11 marker in a wide range of genetic background for early selection of stenospermocarpy in vitis vinifera l.dna markers technology, derived from research in molecular biology and genomics, offers great promise for plant breeding, allowing the "molecular breeding" via marker-assisted selection. grapevine genomic resources allowed, in recent years, the characterization at molecular level of genes involved in interesting phenotypes such as stenospermocarpic seedlessness, a trait really appreciated by consumers. recent studies in table grapes revealed that the vvagl11 gene, member of the d-lineage mads-bo ...201323483354
hydrogen peroxide accumulation and transcriptional changes in grapevines recovered from flavescence dorée disease.flavescence dorée (fd) is considered one of the most severe phytoplasma diseases affecting grapevine. the spontaneous, complete, and stable remission of the symptoms of fd (recovery) is a phenomenon that may occur in infected grapevines. the molecular bases of this phenomenon are still unclear, although some works suggest that recovery could be linked to the accumulation of hydrogen peroxide (h2o2). several genes coding for enzymes involved in h2o2 metabolism, in the ascorbate-glutathione cycle, ...201323489524
differences in the phenolic composition and antioxidant properties between vitis coignetiae and vitis vinifera seeds extracts.phenolic compounds were extracted from european and japanese grapevine species (vitis vinifera and v. coignetiae) seeds using 80% methanol or 80% acetone. the total content of phenolic compounds was determined utilizing folin-ciocalteu's phenol reagent, while the content of tannins was assayed by the vanillin and bsa precipitation methods. additionally, the dpph free radical and abts cation radical scavenging activities and the reduction power of the extracts were measured. the hplc method was a ...201323493104
preventing the acute skin side effects in patients treated with radiotherapy for breast cancer: the use of corneometry in order to evaluate the protective effect of moisturizing creams.the purpose of this study was to add, to the objective evaluation, an instrumental assessment of the skin damage induced by radiation therapy.201323497676
a consensus orthogonal partial least squares discriminant analysis (opls-da) strategy for multiblock omics data fusion.omics approaches have proven their value to provide a broad monitoring of biological systems. however, as no single analytical technique is sufficient to reveal the full biochemical content of complex biological matrices or biofluids, the fusion of information from several data sources has become a decisive issue. omics studies generate an increasing amount of massive data obtained from different analytical devices. these data are usually high dimensional and extracting knowledge from these mult ...201323498118
mineral stress affects the cell wall composition of grapevine (vitis vinifera l.) callus.grapevine (vitis vinifera l.) is one of the most economically important fruit crops in the world. deficit in nitrogen, phosphorus and sulfur nutrition impairs essential metabolic pathways. the influence of mineral stress in the composition of the plant cell wall (cw) has received residual attention. using grapevine callus as a model system, 6 weeks deficiency of those elements caused a significant decrease in growth. callus cws were analyzed by fourier transform infrared spectroscopy (ft-ir), by ...201323498868
vitis vinifera secondary metabolism as affected by sulfate depletion: diagnosis through phenylpropanoid pathway genes and metabolites.grapevine (vitis vinifera l.) is rich in phenylpropanoid compounds, namely flavonoids and stilbenes which, present in most tissues, are described as antioxidants and known to accumulate in response to biotic and abiotic stress. grapevine is then a choice model for studying the interplay between the phenylpropanoid pathway and nutrient deficiency. here we report the response to sulfur deficiency (-s) of flavonoids and stilbenes biosynthetic pathways in chlorophyll tissues (plantlets) and cell cul ...201323500714
manipulating vineyard nitrogen on a saline site: 1. effect of nitrogen on growth, grape yield and nutrients of vitis vinifera l. cv shiraz.with increased prevalence of saline irrigation water applied to vines worldwide, the issue of appropriate nitrogen management is of concern. different rates of nitrogen per vine as urea were applied to shiraz vines on own roots over four seasons in a low-rainfall, saline growing environment.201323504619
overexpression of the grapevine pgip1 in tobacco results in compositional changes in the leaf arabinoxyloglucan network in the absence of fungal infection.constitutive expression of vitis vinifera polygalacturonase-inhibiting protein 1 (vvpgip1) has been shown to protect tobacco plants against botrytis cinerea. evidence points to additional roles for vvpgip1, beyond the classical endopolygalacturonase (epg) inhibition mechanism, in providing protection against fungal infection. gene expression and biochemical datasets previously obtained, in the absence of infection, point to the cell wall, and particularly the xyloglucan component of transgenic v ...201323506352
proteomic analysis indicates massive changes in metabolism prior to the inhibition of growth and photosynthesis of grapevine (vitis vinifera l.) in response to water deficit.cabernet sauvignon grapevines were exposed to a progressive, increasing water defict over 16 days. shoot elongation and photosynthesis were measured for physiological responses to water deficit. the effect of water deficit over time on the abundance of individual proteins in growing shoot tips (including four immature leaves) was analyzed using nanoflow liquid chromatography - tandem mass spectrometry (nanolc-ms/ms).201323514573
Displaying items 1501 - 1600 of 2990