Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| application of variable number of tandem repeats typing to describe familial outbreaks of brucellosis in argentina. | consumption of inadequately pasteurized dairy products is the most common means of transmission of brucellosis. this report describes two foodborne outbreaks that occurred in families infected after consumption of fresh home-made cheese bought in different argentine provinces. high resolution variable number of tandem repeats (vntr)-based analysis revealed two well-defined groups comprising essentially identical profiles and corresponding to the two different outbreaks. similar clinical findings ... | 2010 | 20167681 |
| atp-binding cassette systems of brucella. | brucellosis is a prevalent zoonotic disease and is endemic in the middle east, south america, and other areas of the world. in this study, complete inventories of putative functional abc systems of five brucella species have been compiled and compared. abc systems of brucella melitensis 16m, brucella abortus 9-941, brucella canis rm6/66, brucella suis 1330, and brucella ovis 63/290 were identified and aligned. high numbers of abc systems, particularly nutrient importers, were found in all brucel ... | 2010 | 20169092 |
| the development of oral fluid-based diagnostics and applications in veterinary medicine. | the purpose of this review was to discuss the history of the development and implementation of oral fluid diagnostics for infectious diseases of humans and domestic animals. the use of oral fluid for the assessment of health and diagnosis of disease in humans and animals has a surprisingly long history. as early as 1909, pollaci and ceraulo reported sensitive and specific agglutination of 'micrococcus melitensis' (brucella melitensis) by oral fluid from patients diagnosed with malta fever. a 198 ... | 2010 | 20202287 |
| spatial distribution and risk factors of brucellosis in iberian wild ungulates. | the role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. the aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several iberian wild ungulates. | 2010 | 20205703 |
| isolation of brucella melitensis from cow's milk. | 1947 | 20344059 | |
| evaluating the virulence of a brucella melitensis hemagglutinin gene in the caprine model. | with the completion of the genomic sequence of brucella melitensis 16m, a putative hemagglutinin gene was identified which is present in 16m and absent in brucella abortus. the possibility of this hemagglutinin being a potential virulence factor was evaluated via gene replacement in b. melitensis yielding 16mδe and expression in trans in b. abortus 2308-qae. utilizing the caprine brucellosis model, colonization and pathogenesis studies were performed to evaluate these strains. b. melitensis 16m ... | 2010 | 20362205 |
| attenuation of defined brucella melitensis wboa mutants. | rough brucella mutants have been sought as vaccine candidates because they do not induce seroconversion. in this study, two defined nonreverting rough mutants were derived from virulent brucella melitensis strain 16m: a wboa deletion mutant designated wrr51 and a wboa purek dual deletion mutant designated wrrp1. strain wrrp1 exhibited reduced survival in human monocyte-derived macrophages (hmdms) compared with parent strain wrr51 or with δpurek strain wr201. strain wrrp1 persisted for 1 week or ... | 2010 | 20362622 |
| risk factors for in-hospital mortality in infective endocarditis: five years' experience at a tertiary care hospital in turkey. | despite recent improvements in diagnostic and therapeutic interventions, infective endocarditis (ie) is still associated with high in-hospital mortality rates. the study aim was to determine the clinical, laboratory and echocardiographic features of ie, and to evaluate the risk factors for in-hospital mortality. | 2010 | 20369506 |
| global analysis of quorum sensing targets in the intracellular pathogen brucella melitensis 16 m. | many pathogenic bacteria use a regulatory process termed quorum sensing (qs) to produce and detect small diffusible molecules to synchronize gene expression within a population. in gram-negative bacteria, the detection of, and response to, these molecules depends on transcriptional regulators belonging to the luxr family. such a system has been discovered in the intracellular pathogen brucella melitensis, a gram-negative bacterium responsible for brucellosis, a worldwide zoonosis that remains a ... | 2010 | 20387905 |
| [chronic brucellosis: a rare cause of septic loosening of arthroplasties with high risk of laboratory-acquired infections]. | brucella spp. are a main contributor to morbidity in endemic countries, especially in the near and middle east and the mediterranean. however, in most western industrialised countries, cases are rare. here, we report the case of a previously unrecognised chronic brucellosis with subsequent laboratory-acquired infection. a 64-year-old female presented to the orthopaedic department with loosening of her knee prosthesis after total knee arthroplasty five years earlier. bacterial cultures were obtai ... | 2011 | 20419627 |
| an indirect elisa for the detection of antibody in milk from sheep experimentally infected with brucella melitensis biovar 3. | an indirect elisa was evaluated for the detection of brucella antibodies in milk (m-elisa) from sheep experimentally infected with b. melitensis biovar 3. at the end of the second reproductive cycle (13 months post infection), the milk of 22 lactating sheep was tested using the m-elisa. sera from the same sheep were also tested for brucella antibodies using the rose bengal test (rbt) and the complement fixation test (cft). the first serum sampling after parturition showed 100% sensitivity in bot ... | 2006 | 20429056 |
| kinetics of the antibody response in ewes experimentally infected with brucella melitensis biovar 3. | the authors evaluated the kinetics of antibody response in 46 ewes coming from officially brucellosis free flocks that were experimentally infected with brucella melitensis biovar 3, and monitored through three subsequent reproductive cycles. in this study, results of rose bengal test (rbt) and complement fixation test (cft) were considered. test results of 2nd and 3rd reproductive cycle show a peak in the antibody production at parturition, followed by a drop in the following months. the peak a ... | 2004 | 20437389 |
| a 43-year-old woman with abdominal pain and fever. | 2010 | 20440575 | |
| large scale immune profiling of infected humans and goats reveals differential recognition of brucella melitensis antigens. | brucellosis is a widespread zoonotic disease that is also a potential agent of bioterrorism. current serological assays to diagnose human brucellosis in clinical settings are based on detection of agglutinating anti-lps antibodies. to better understand the universe of antibody responses that develop after b. melitensis infection, a protein microarray was fabricated containing 1,406 predicted b. melitensis proteins. the array was probed with sera from experimentally infected goats and naturally i ... | 2010 | 20454614 |
| biochemical and functional analysis of tir domain containing protein from brucella melitensis. | toll/interleukin-1 like receptors are evolutionarily conserved proteins in eukaryotes that play crucial role in pathogen recognition and innate immune responses. brucella are facultative intracellular bacterial pathogens causing brucellosis in animal and human hosts. brucella behave as a stealthy pathogen by evading the immune recognition or suppressing the tlr signaling cascades. brucella encode a tir domain containing protein, tcpb, which suppresses nf-kappab activation as well as pro-inflamma ... | 2010 | 20471373 |
| on the resistance of the micrococcus melitensis to moist heat: suggested "standard" methods in the determination of thermal death points. | 1904 | 20474189 | |
| brucella melitensis 16m produces a mannan and other extracellular matrix components typical of a biofilm. | mutations in the brucella melitensis quorum-sensing (qs) system are involved in the formation of clumps containing an exopolysaccharide. here, we show that the overexpression of a gene called aiid in b. melitensis gives rise to a similar clumping phenotype. the aiid enzyme degrades ahl molecules and leads therefore to a qs-deficient strain. we demonstrated the presence of exopolysaccharide and dna, two classical components of extracellular matrices, in clumps produced by this strain. we also obs ... | 2010 | 20497223 |
| brucella melitensis vjbr and c12-hsl regulons: contributions of the n-dodecanoyl homoserine lactone signaling molecule and luxr homologue vjbr to gene expression. | quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. deletion of the luxr homologue vjbr highly attenuates intracellular survival of brucella melitensis and has been interpreted to be an indication of a role for qs in brucella infection. confirmation for such a role was suggested, but not confirmed, by the demonstrated in vitro synthesis of an auto-inducer (ai) by brucella cultures. in an effort to f ... | 2010 | 20529360 |
| epidemiological and phylogenetic analysis of spanish human brucella melitensis strains by multiple-locus variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpob typing. | the severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. multiple-locus variable-number repeat analysis (mlva-16), hypervariable octameric oligonucleotide fingerprinting (hoof-print), and the differences in the single nucleotide polymorphisms (snps) (codons 1249 and 1309) of the dna-dependent rna polymerase beta subunit (rpob) were used to type a human brucella melitensis population (108 strain ... | 2010 | 20554816 |
| evaluation of in vitro activities of tigecycline and various antibiotics against brucella spp. | brucellosis is a zoonosis with a worldwide distribution and remains a significant public health problem mainly in the developing world. in this study we evaluated the in vitro activities and synergistic effects of antibiotic combinations against blood culture isolates of brucella spp. in vitro susceptibilities of 76 blood culture isolates of brucella melitensis and one blood culture isolate of brucella abortus to doxycycline, streptomycin, gentamicin, trimethoprim-sulfamethoxazole, moxifloxacin, ... | 2010 | 20568531 |
| differences in the outer membrane-related properties of the six classical brucella species. | outer membrane-related properties (such as auto-agglutination and susceptibility to various compounds) of strains representative of the six classical species of the genus brucella were assessed. the differences identified could not be fully explained based on the smooth or rough phenotype of the strain. smooth strains of the closely related species brucella melitensis and b. abortus exhibited different susceptibility patterns and the rough, virulent b. ovis and b. canis strains were equally or m ... | 2010 | 20576453 |
| management of an outbreak of brucellosis due to b. melitensis in dairy cattle in spain. | brucella melitensis is a major human and animal pathogen, with a wide host range that includes all domestic ruminant species, although small ruminants are its preferred hosts. outbreaks in cattle due to b. melitensis have become a worldwide emerging problem particularly difficult to control due to the lack of knowledge on the epidemiology in this host species and of an effective vaccine. however, combination of molecular tools and strict biosecurity measures can help to solve these difficulties ... | 2011 | 20579679 |
| brucellosis infection presenting with cholestasis. | brucellosis is a relatively common disease in the mediterranean area and may present with prolonged fever without focus, however it remains an important diagnostic challenge to most pediatricians. we report the case of a 10-year-old male patient who presented with fever without a focus of 10-day duration, hepatomegaly, ascites, a small elevation in transaminases and acute-phase reactants indicating cholestasis, leukopenia, and thrombocytopenia. imaging tests showed many small, rounded, hypodense ... | 2010 | 20579915 |
| brucellosis in a patient with end-stage liver disease undergoing liver transplantation: successful treatment with tigecycline. | 2010 | 20589653 | |
| in vitro assay for the anti-brucella activity of medicinal plants against tetracycline-resistant brucella melitensis. | brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. as an alternative to conventional antibiotics, medicinal plants are valuable resources for new agents against antibiotic-resistant strains. the aim of this study was to investigate the usage of native plants for brucellosis treatment. for this purpose, the anti-brucella activities of ethanolic and methanolic extracts o ... | 2010 | 20593515 |
| disseminated intravascular coagulation in a case of brucellosis. | brucellosis is a multisystem disease with a broad spectrum of clinical manifestations; hematological abnormalities ranging from fulminant as of disseminated intravascular coagulation (dic) to anaemia, leucopoenia, thrombocytopenia, and clotting disorders. in this report, we present dic in a case of brucellosis because of rare presentation. a 3-year-old boy was admitted with the complaints of continuous fever, vomiting, abdominal pain, and bruise on leg. he looked pale and his physical examinatio ... | 2010 | 20682596 |
| diagnostic challenges of human brucellosis in hong kong: a case series in two regional hospitals. | a retrospective analysis of six patients diagnosed with brucellosis in two regional hospitals was carried out. the epidemiological, clinical, and laboratory features were studied. all patients had exposure history. three patients presented with musculoskeletal symptoms, while three had predominantly genitourinary symptoms. one patient did not have fever at presentation. all patients were diagnosed by positive blood culture of brucella melitensis, and the diagnosis was not suspected for all excep ... | 2010 | 20683074 |
| a rare case of seronegative neurobrucellosis. | neurobrucellosis is one of the complications of brucellosis. we report a rare case of a 17-year-old girl with seronegative neurobrucellosis and depression and diplopia. results of agglutination tests for brucella both in serum and csf were negative. diagnosis was made only by positive culture of brucella mellitensis with inoculation of the patient's cerebrospinal fluid in a bactec 9050 system. the patient was successfully treated using ceftriaxone, doxycycline and rifampicin therapy for six mont ... | 2010 | 20697164 |
| neurobrucellosis associated with syndrome of inappropriate antidiuretic hormone with resultant diabetes insipidus and hypothyroidism. | neurological involvement of the central nervous system in brucellosis is uncommon. we describe a rare case of meningoencephalitis due to brucella melitensis infection, associated with the syndrome of inappropriate antidiuretic hormone secretion and leading to diabetes insipidus and hypothyroidism. neurobrucellosis, although rare, should be considered in cases of neurological disease of unknown etiology. | 2010 | 20702671 |
| epidemiological characteristics of brucellosis in sheep and goats in bulgaria: 2005-2008. | (full text is available at http://www.manu.edu.mk/prilozi). aim: to analyse and present the epidemiological and epizootological situation of brucellosis in humans, sheep and goats caused by brucella melitensis. methods: the veterinary health services analyzsd epidemiological reports, as well as other official documents, in relation to the epidemiology and the measures undertaken for control of brucellosis in bulgaria, 2005-08. results: in bulgaria animal infections caused by brucella melitensis ... | 2010 | 20703183 |
| diagnostic validation of ovine and caprine brucellosis using serum- and milk-elisa. | (full text is available at http://www.manu.edu.mk/prilozi). aim: to introduce and validate the new method of diagnosing ovine and caprine brucellosis in a rapid, accurate and inexpensive manner by using i-elisa (serum/milk) technique. methods: serum and milk samples from brucella rb and cft negative (n = 881) and positive (n = 755) animals were used. standardization of tests was through the bommeli elisa-besw (brucella bang) standard and our institute's (mkd) working standards (positive serum an ... | 2010 | 20703186 |
| evaluation of the fluorescence polarization assay (fpa) for diagnosis of brucella melitensis infection of goats in argentina. | (full text is available at http://www.manu.edu.mk/prilozi). aim: determine the optimal cut-off and the use of fluorescence polarization assay (fpa) to improve the detection of brucellosis in individual goats in argentina. methods: sera from 96 goats from a flock with abortion due to b. melitensis biovar 1 were used to assess the efficacy of the fpa to detect brucellosis in goats. fpa results were compared with those of the buffered antigen plate agglutination test (bpat) confirmed by seroaggluti ... | 2010 | 20703188 |
| control and eradication strategies for brucella melitensis infection in sheep and goats. | (full text is available at http://www.manu.edu.mk/prilozi). aim: to describe the various strategies for the control and eradication of b. melitensis infection in sheep and goats. methods: the advantages and drawbacks of these strategies are discussed on the basis of the author's personal experience and a revision of the relevant literature. results: vaccination programmes in various combinations can be applied either to decrease the prevalence of infection in the animal population or, when combi ... | 2010 | 20703189 |
| brucellosis as priority public health challenge in south eastern european countries. | 2010 | 20718078 | |
| serological, bacteriological, and molecular diagnosis of brucellosis in domestic animals in croatia. | to present the surveillance data on brucella melitensis, b. suis, and b. ovis infection in cattle, sheep, goats, and swine in croatia obtained in 2008 by serological, bacteriological, and molecular methods for diagnostics of brucellosis in domestic animals. | 2010 | 20718085 |
| bone abscesses due to brucella melitensis. | 1932 | 20776941 | |
| [spinal infections: typical and atypical imaging features]. | while the imaging features of established spondylodiscitis are well known, other presentations may be misleading. the purpose of this article is to review the typical imaging features of spondylodiscitis as well as some more atypical and unusual imaging features (early spondylodiscitis, sceptic spondylitis, septic arthritis of facet joints, primary epidural abscess, unusual germs, neonate). | 2010 | 20814395 |
| acute acalculus cholecystitis and hepatitis caused by brucella melitensis. | acute cholecystitis is a very rare presentation of brucellosis. a case of acalculous cholecystitis caused by brucella melitensis is reported with a review of previously reported cases. | 2010 | 20818096 |
| mlva typing and antibiotic susceptibility of brucella human isolates from liaoning, china. | we have characterized clinical isolates of brucella melitensis from liaoning, china, using multiple-locus variable-number repeat analysis of 16 loci (mlva-16) and evaluated the antimicrobial susceptibilities in vitro. all 31 isolates shared the panel 1 genotype 42 profile suggesting that they belong to the 'east mediterranean' group. in vitro antibiotic susceptibility was determined by the broth microdilution testing. all isolates were sensitive to tested antibiotics (rifampin, doxycycline, cipr ... | 2010 | 20832094 |
| functional characterization of the incomplete phosphotransferase system (pts) of the intracellular pathogen brucella melitensis. | in many bacteria, the phosphotransferase system (pts) is a key player in the regulation of the assimilation of alternative carbon sources notably through catabolic repression. the intracellular pathogens brucella spp. possess four pts proteins (eintr, npr, eiiantr and an eiia of the mannose family) but no pts permease suggesting that this pts might serve only regulatory functions. | 2010 | 20844759 |
| brucella melitensis: a rare cause of febrile neutropenia. | although brucellosis is endemic in middle eastern and mediterranean countries, febrile neutropenia caused by brucella species has rarely been reported in childhood. in this report the authors described an unusual case of febrile neutropenia due to brucella melitensis. | 2011 | 20863159 |
| coincidence of acute brucella hepatitis and dengue fever or serologic cross-reactivity? | hepatic involvement in brucellosis is not uncommon since 10-20% of patient infected with brucella species can have abnormal liver function tests. the usual presentation of brucella hepatitis is in the form of chronic granulomatous hepatitis with mild to moderate elevation of liver enzymes, while acute hepatitis is rare. we report a young patient who presented with acute brucella-induced hepatitis and co-infection with dengue hemorrhagic virus resulting in severe elevation of liver enzymes and ab ... | 2010 | 20871199 |
| selective amplification of brucella melitensis mrna from a mixed host-pathogen total rna. | abstract: | 2010 | 20920187 |
| identification of the essential brucella melitensis porin omp2b as a suppressor of bax-induced cell death in yeast in a genome-wide screening. | inhibition of apoptosis is one of the mechanisms selected by numerous intracellular pathogenic bacteria to control their host cell. brucellae, which are the causative agent of a worldwide zoonosis, prevent apoptosis of infected cells, probably to support survival of their replication niche. | 2010 | 20949000 |
| genomic island 2 is an unstable genetic element contributing to brucella lipopolysaccharide spontaneous smooth-to-rough dissociation. | brucella is a gram-negative bacterium that causes a worldwide-distributed zoonosis. the genus includes smooth (s) and rough (r) species that differ in the presence or absence, respectively, of the o-polysaccharide of lipopolysaccharide. in s brucellae, the o-polysaccharide is a critical diagnostic antigen and a virulence determinant. however, s brucellae spontaneously dissociate into r forms, a problem in antigen and s vaccine production. spontaneous r mutants of brucella abortus, brucella melit ... | 2010 | 20952568 |
| the effect of tigecycline and ertapenem against clinical isolates of brucella melitensis detected by e-test on different media. | in this study, in vitro activity of tigecycline (tig) and ertapenem (ert) against clinical isolates of brucella melitensis and the effect of different media on in vitro test results were investigated. the in vitro effects of tig and ert to 38 b. melitensis isolates were comparatively investigated in brucella agar and 5% sheep blood agar. mic value of ert was 0.032 μg/ml in 23 of 38 and 20 of 38 isolates on blood and brucella agar, respectively. minimum inhibitory concentration values of tig were ... | 2010 | 20966572 |
| brucella melitensis infections in iowa. | 1946 | 21000813 | |
| occurrence of brucella melitensis in iowa. | 1946 | 21008655 | |
| brucella melitensis isolated from swine tissues in iowa. | 1946 | 21019101 | |
| ovine and caprine brucellosis (brucella melitensis) in aborted animals in jordanian sheep and goat flocks. | two hundred and fifty five biological samples were collected from 188 animals (81 sheep and 107 goats) during the lambing season from september 2009 to april 2010 from the mafraq region of jordan. sampled animals belonged to 93 sheep and goat flocks that had abortion cases in the region. one hundred and seven (41.9%) biological samples were positive for the omp2 primers that were able to identify all brucella species in the collected samples which were obtained from 86 aborted animals (86/188 = ... | 2010 | 21052561 |
| immunization of mice with gamma-irradiated brucella neotomae and its recombinant strains induces protection against virulent b. abortus, b. melitensis, and b. suis challenge. | human brucellosis, a zoonotic disease of major public health concern in several developing countries, is primarily caused by brucella abortus, brucella melitensis, and brucella suis. no brucellosis vaccine is available for human use. the aim of this study was to determine if brucella neotomae, a bacterium not known to cause disease in any host, can be used for developing brucellosis vaccines. b. neotomae and its recombinant strains overexpressing superoxide dismutase and a 26 kda periplasmic pro ... | 2010 | 21109033 |
| novel bioactive hydrophobic gentamicin carriers for the treatment of intracellular bacterial infections. | gentamicin (gen) is an aminoglycoside antibiotic with a potent antibacterial activity against a wide variety of bacteria. however, its poor cellular penetration limits its use in the treatment of infections caused by intracellular pathogens. one potential strategy to overcome this problem is the use of particulate carriers that can target the intracellular sites of infection. in this study gen was ion-paired with the anionic aot surfactant to obtain a hydrophobic complex (gen-aot) that was formu ... | 2010 | 21115143 |
| brucellosis reactivation after 28 years. | 2010 | 21122256 | |
| morphological analysis of the sheathed flagellum of brucella melitensis. | abstract: | 2010 | 21143933 |
| identification of brucella by maldi-tof mass spectrometry. fast and reliable identification from agar plates and blood cultures. | maldi-tof mass spectrometry (ms) is a reliable method for bacteria identification. some databases used for this purpose lack reference profiles for brucella species, which is still an important pathogen in wide areas around the world. we report the creation of profiles for maldi-tof biotyper 2.0 database (bruker daltonics, germany) and their usefulness for identifying brucellae from culture plates and blood cultures. | 2010 | 21151913 |
| alimentary tract presentations of brucellosis. | a retrospective study was conducted to assess alimentary tract involvement in 140 saudi patients with acute or chronic brucellosis. a brucella standard agglutination titer of 160 or over was considered to be positive evidence of brucella infection. the commonest symptoms were anorexia, weight loss, right hypochondrial pain, and constipation. a history of raw milk ingestion was obtained in 38 patients. thirty-nine patients had hepatomegaly, 21 had splenomegaly, and six had signs of cholecystitis. ... | 1986 | 21164236 |
| immunization of mice with recombinant s-adenosyl-l-homocysteine hydrolase protein confers protection against brucella melitensis infection. | due to drawbacks of live attenuated vaccines, much attention has been focused on screening brucella-protective antigens as subunit vaccine candidates. here, an immunoproteomic assay was used to identify the immunogenic soluble proteins of brucella melitensis 16m. in the present study, 27 unique immunogenic proteins were identified from the two-dimensional electrophoresis immunoblot profiles by liquid chromatography tandem ms (lc-ms/ms). from this set, the gene encoding one immunodominant protein ... | 2010 | 21166726 |
| antimicrobial susceptibility of brucella melitensis isolates in peru. | brucellosis is an important public health problem in peru. we evaluated 48 human brucella melitensis biotype 1 strains from peru between 2000 and 2006. mics of isolates to doxycycline, azithromycin, gentamicin, rifampin, ciprofloxacin, and trimethoprim-sulfamethoxazole were determined by the etest method. all isolates were sensitive to tested drugs during the periods of testing. relapses did not appear to be related to drug resistance. | 2011 | 21199926 |
| control and eradication of brucella melitensis infection in sheep and goats. | brucella melitensis is the main etiological agent of brucellosis in sheep and goats, and is also the main agent responsible for human brucellosis, a predominantly occupational disease related to professions in direct contact with livestock. as there is currently no viable method of preventing human brucellosis to safeguard people attention must be directed toward effectively controlling the disease in sheep and goats. this review focuses on the different strategies in different socioeconomic and ... | 2011 | 21215893 |
| assessment of milk ring test and some serological tests in the detection of brucella melitensis in syrian female sheep. | brucella melitensis infection prevalence among syrian female sheep, to evaluate a number of serological tests and to discuss some epidemiological aspects of brucellosis, was studied. a total of 2,580 unvaccinated syrian female sheep sera samples were tested for b. melitensis antibodies detection using four serological methods: the rose bengal test (rbt), the serum agglutination test (sat), the complement fixation test (cft) and an indirect enzyme-linked immunosorbent assay (ielisa). in addition, ... | 2011 | 21234678 |
| rpoe1, an extracytoplasmic function sigma factor, is a repressor of the flagellar system in brucella melitensis. | the genome of brucella melitensis contains genes coding for the sigma factors rpod, rpon, rpoh1, rpoh2, rpoe1 and rpoe2. previously published data show that b. melitensis is flagellated and that an rpoe1 mutant overexpresses the flagellar protein flge. in this study, we demonstrate that mutation of rpoe1 causes an overexpression of the flagellar genes flif, flge, flic, flaf and flbt, correlating with the production of a longer filament and thereby demonstrating that rpoe1 acts as a flagellar rep ... | 2011 | 21273248 |
| role of flbt in flagellin production in brucella melitensis. | it was recently demonstrated that the pathogen brucella melitensis produces a polar sheathed flagellum under the control of the master regulator ftcr. however, the regulatory mechanism controlling the flagellar assembly remains unknown. in this work, we investigate the flagellar hierarchy of b. melitensis as well as the flagellin flic regulation. we show that a mutation in flif or flge (coding for the basal body structure and the hook, respectively) does not affect flic synthesis, suggesting tha ... | 2011 | 21273249 |
| brucellosis acquired by eating imported cheese. | pyrexia of unknown origin is an important clinical presentation in both paediatric and adult medicine. we present a case of pyrexia of unknown origin in a 14 year-old boy which turned out to be due to infection with brucella melitensis, despite the patient not having left great britain - an officially brucellosis-free country - in six years. repeated history-taking provided a clue to the diagnosis. | 2011 | 21276115 |
| b. melitensis rough strain b115 is protective against heterologous brucella spp. infections. | brucellosis is one of the most serious zoonoses all over the world, with b. melitensis, b. abortus and b. suis being the most pathogenic species for humans. vaccination of domesticated livestock still represents the most efficient way to prevent human infection. however, the available brucella vaccines retain an important residual virulence and induce antibodies interfering with surveillance programs. moreover, each vaccine shows different protective effects versus different brucella species and ... | 2011 | 21300102 |
| splenic abscess due to brucella melitensis - a rare pediatric complication. | splenic abscess due to brucella species is an extremely rare complication especially in acute illness. here we report a case of splenic abscess caused by brucella melitensis biotype 1 in a child with acute infection who was successfully treated with only antibiotics. | 2010 | 21346907 |
| chronic, progressive back pain, fever and a noticeable paravertebral mass. | 2011 | 21363970 | |
| imported brucellosis in denmark: molecular identification and multiple-locus variable number tandem repeat analysis (mlva) genotyping of the bacteria. | a polymerase chain reaction was used to identify brucella species isolated from humans in denmark. consecutive analysis of referred bacteria and re-examination of historical isolates identified all as brucella melitensis. multiple-locus variable number tandem repeat analysis (mlva) placed the isolates in the previously defined 'east mediterranean' b. melitensis group. | 2011 | 21375425 |
| brucellosis in pregnancy. | this study was undertaken in order to evaluate the effect of brucellosis in pregnancy. the serum agglutination test, coombs and/or blood culture systems were used in the diagnosis of brucellosis. from july 2003 to september 2010, the clinical and delivery patterns of 40 healthy pregnant women were compared with 39 pregnant women who had brucellosis. there were no birth defects, anomalies or mortalities. we observed that brucellosis in pregnancy increases the incidence of preterm delivery (p = 0. ... | 2011 | 21378061 |
| an analysis of children with brucellosis associated with pancytopenia. | brucellosis produces a variety of nonspecific hematologic abnormalities. hematologic complications of mild anemia and leukopenia have been frequently associated with acute brucellosis, but pancytopenia is less frequently seen. in this study, records of children with brucellosis aged under or equal to 16 years, admitted to yuzuncu yil university hospital between 2004 and 2010, were analyzed retrospectively. over this time period, 187 patients with brucellosis were diagnosed. twenty-five (13.3%) o ... | 2011 | 21381870 |
| association between brucella melitensis dna and brucella sp. antibodies. | 2011 | 21411601 | |
| peculiarities of the regulation of the brucella flagellum. | 2011 | 21415118 | |
| microangiopathic anemia of acute brucellosis - is it a true ttp? | thrombotic thrombocytopenic purpura (ttp) is a severe disease, potentially fatal, if not diagnosed and treated promptly. ttp is clinically characterized by the pentad of thrombocytopenia, coombs-negative hemolytic anemia, fever, renal abnormalities and neurological disturbances. advances in recent years have delineated the molecular mechanisms of acquired and hereditary ttp.many infectious organisms have been reported to be associated with ttp, especially mycoplasma, but few cases of brucella in ... | 2010 | 21415981 |
| [pet-ct scan in a case of suspicion of metastatic dissemination due to the presence of multiple pulmonary nodules]. | 2011 | 21440334 | |
| expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of brucella melitensis m5-90: a potential antigen for differential serodiagnosis of brucellosis in sheep and goats. | antibodies produced in animals vaccinated using live attenuated vaccines against brucella spp. are indistinguishable using current conventional serological tests from those produced in infected animals. one potential approach is to develop marker vaccines in which specific genes have been deleted from parental vaccine strains that show good immunogenicity and vaccine efficacy. corresponding methods of detection for antibodies raised by the marker vaccine should also be developed. a specific frag ... | 2011 | 21446957 |
| antimicrobial susceptibilities of brucella isolates from various clinical specimens. | brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. in the study we claimed to identify brucella species from clinical samples of patients with active brucellosis from van region of eastern anatolia and to determine in vitro antimicrobial susceptibilities of these strains to commonly used anti-brucella agents and a possible new alternative tigecycline. | 2011 | 21448305 |
| brucellosis presenting as piriformis myositis: a case report. | 2011 | 21450058 | |
| isolation of brucella melitensis from a rb51-vaccinated seronegative goat. | the purpose of this study is to determine the etiology of abortions presented in a goat herd declared as free of brucellosis and vaccinated with rb51 located in mexico. the serological diagnosis of brucellosis in 33 animals was performed. the study included three goats that aborted in the last third of gestation and 15 goats that gave birth normally; samples of milk and vaginal exudate were subjected to bacteriological study. all animals were negative for serological diagnosis, and isolation of ... | 2011 | 21455694 |
| bilateral pulmonary nodules due to brucellosis. | 2011 | 21470756 | |
| complete genome sequences of brucella melitensis strains m28 and m5-90, with different virulence backgrounds. | brucella melitensis is a gram-negative coccobacillus bacteria belonging to the alphaproteobacteria subclass. it is an important zoonotic pathogen that causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. the b. melitensis strain m5-90, a live attenuated vaccine cultured from the b. melitensis virulent strain m28, has been an effective tool to control brucellosis in goats and sheep in china. here we report the complete genome sequences of b. melitensis m28 and m5-90, strai ... | 2011 | 21478357 |
| endovascular repair of aortic rupture due to brucella aortitis. | brucellosis is a zoonosis, caused by bacteria belonging to the genus brucella. aortic involvement is a rare complication, often following embolization from infective endocarditis. however, contiguous propagation from vertebral involvement may occur. we report the case of an 81 year old patient abruptly presenting with aortic rupture due to brucella melitensis infection. the diagnosis of aortic rupture was made by ct. the patient underwent urgent endovascular treatment using endoprosthesis deploy ... | 2011 | 21500181 |
| the 16mδvjbr as an ideal live attenuated vaccine candidate for differentiation between brucella vaccination and infection. | brucellosis brings great economic burdens for developing countries. live attenuated vaccines are the most efficient means for prevention and control of animal brucellosis. however, the difficulties of differentiating of infection from vaccine immunization, which is essential for eradication programs, limit their applications. therefore, the development of a vaccine that could differentiate infection from immunization will overcome the limitations and get extensive application. vjbr is a quorum s ... | 2011 | 21530111 |
| [direct detection of the brucellosis-causing pathogen--a case report]. | bacterial zoonoses still represent a serious medical problem. one of the less frequent but severe zoonoses is brucellosis caused by the bacterium brucella melitensis. the presented case report describes relapsing imported brucellosis in a young male. in addition to four serological tests, the diagnosis was confirmed by direct detection of the pathogen in blood culture. the isolate of brucella melitensis was identified using the maldi-tof biotyper method and subsequently also by pcr. | 2011 | 21574131 |
| evaluation of a nanobody phage display library constructed from a brucella-immunised camel. | brucella are invasive gram-negative bacteria that multiply and survive within eukaryotic cells causing brucellosis. syrian (and middle east) health and economy sectors are still affected by this disease causing a serious national problem that needs to be solved. here, a strategy was developed to introduce a new generation of binders, known as nanobodies (nbs) in our combat against brucella. these nbs, recombinant single-domain variable fragments derived from camelid heavy-chain antibodies are ve ... | 2011 | 21592585 |
| the whole genome sequences of four brucella strains. | brucella melitensis and brucella suis are intracellular pathogens to livestock and humans. here we report four genome sequences, the virulent strain b. melitensis m28-12 and vaccine strains b. melitensis m5, m111 and b. suis s2 that show varied virulence and pathogenicity, which will help to design more effective brucellosis vaccine. | 2011 | 21602346 |
| soluble expression and purification of brucella cell surface protein (bcsp31) of brucella melitensis and preparation of anti-bcsp31 monoclonal antibodies. | brucella cell surface protein (bcsp31) is potentially useful for diagnosing brucellosis. we aimed to establish a monoclonal antibody (mab) against brucella melitensis bcsp31 and to investigate its distribution in diagnosis. soluble recombinant bcsp31 was successfully expressed and purified. two mabs (1f1 and 1e5) against b. melitensis bcsp31, effective in detecting both recombinant and cellular proteins, were obtained and characterized. the mabs did not react with escherichia coli, staphylococcu ... | 2011 | 21603860 |
| comparison of different pcr methods for detection of brucella spp. in human blood samples. | for detection of brucella species by pcr four dna extraction methods and four targets were compared using pure culture of brucella melitensis and the best conditions were applied in clinical samples. it was found that the magna pure lc method was the most efficient and sensitive method showing a positive pcr reaction with dna extracted from as low as 25 and 100 cfu suspended in one ml blood and one ml water, respectively. detection of brucella spp. by conventional pcr was investigated using four ... | 2011 | 21630571 |
| brucellosis in camels. | camels are highly susceptible to brucellosis caused by brucella melitensis and brucella abortus. difficulties can arise in diagnosis of camel brucellosis, especially as this disease provokes only few clinical signs in contrast to its clinical course in cattle. because none of the commonly used serological test can be perceived as a perfect test for brucella diagnosis in camel and most serological tests used for camels have been directly transposed from cattle without adequate validation, an inco ... | 2011 | 21632084 |
| multi-comparative systems biology analysis reveals time-course biosignatures of in vivo bovine pathway responses to b.melitensis, s.enterica typhimurium and m.avium paratuberculosis. | to decipher the complexity and improve the understanding of host-pathogen interactions, biologists must adopt new system level approaches in which the hierarchy of biological interactions and dynamics can be studied. this paper presents the application of systems biology for the cross-comparative analysis and interactome modeling of three different infectious agents, leading to the identification of novel, unique and common molecular host responses (biosignatures). | 2011 | 21645321 |
| molecular typing of brucella species isolates from livestock and human. | although host specificity has been observed in different species of brucella, crossing the animal host boundary is likely to occur at any time. in this study, bruce ladder pcr and abortus-melitensis-ovis-suis (amos) pcr assays were used to characterize 47 brucella isolates from indian origin in order to know exact species for understanding epidemiology of brucellosis. out of them, 28, 14, and 5 isolates were found to be brucella abortus, brucella melitensis, and brucella suis, respectively. furt ... | 2011 | 21647774 |
| enhancing the role of veterinary vaccines reducing zoonotic diseases of humans: linking systems biology with vaccine development. | the aim of research on infectious diseases is their prevention, and brucellosis and salmonellosis as such are classic examples of worldwide zoonoses for application of a systems biology approach for enhanced rational vaccine development. when used optimally, vaccines prevent disease manifestations, reduce transmission of disease, decrease the need for pharmaceutical intervention, and improve the health and welfare of animals, as well as indirectly protecting against zoonotic diseases of people. ... | 2011 | 21651944 |
| infection of cattle in kenya with brucella abortus biovar 3 and brucella melitensis biovar 1 genotypes. | brucella melitensis biovar 1 was isolated from bovine milk samples from a herd in central kenya, and brucella abortus biovar 3 was isolated from aborted fetus materials and vaginal discharge fluids from cattle in central and eastern provinces of kenya. all infections including those with b. melitensis were in cattle with reproductive problems kept in mixed herds indicating that cross infection occurs from small ruminants. multiple-locus variable-number tandem repeat analysis genotyping revealed ... | 2011 | 21660647 |
| acute prostatitis as an uncommon presentation of brucellosis. | the present report concerns a 46-year-old man who presented with acute prostatitis due to brucella melitensis infection. he was first treated with doxycycline and ciprofloxacin, but after 3 months he was admitted again with the same diagnosis. the relapse was probably related to ciprofloxacin use, or the length of treatment not being sufficient. the patient was successfully treated with a combination of doxycycline and rifampin for 3 months. in conclusion, prostatitis due to brucella, such as sp ... | 2009 | 21686349 |
| a case of brucellosis presenting with multiple hypodense splenic lesions and bilateral pleural effusions. | brucellosis is a zoonotic infectious disease, which mainly present with lymphoreticular system invovement. however any organ system can be attacked by the microorganism. in this paper we present a 52-year-old female patient who was admitted to the infectious diseases department with complaints of fatigue, arthralgias, fever, and weight loss. in the medical examination and radiological analysis bilateral pleural effusions and hepatosplenomegaly were detected. serum transaminase levels were two ti ... | 2011 | 21687542 |
| modulation of microtubule dynamics by a tir domain protein from the intracellular pathogen, brucella melitensis. | tir domain containing proteins play a crucial role in innate immunity in eukaryotes. brucella is a highly infectious intracellular bacterium that encodes a tir domain protein (tcpb) to subvert host innate immune responses to establish a beneficial niche for pathogenesis. tcpb inhibits nf-+¦b activation and pro-inflammatory cytokine secretions mediated by toll like receptor (tlr) 2 & 4. here, we demonstrate that tcpb modulates microtubule dynamics by acting as a stabilization factor. tcpb increas ... | 2011 | 21692747 |
| protective efficacy and safety of brucella melitensis 16m{delta}mucr against intraperitoneal and aerosol challenge in balb/c mice. | brucellosis is a zoonosis of nearly worldwide distribution. vaccination against this pathogen is an important control strategy to prevent the disease. currently licensed vaccine strains used in animals are unacceptable for human use due to undesirable side effects and modest protection. substantial progress has been made during the past 10 years toward the development of improved vaccines for brucellosis. in part, this has been achieved by the identification and characterization of live attenuat ... | 2011 | 21708998 |
| isolation rates of brucella melitensis in an endemic area and implications for laboratory safety. | a retrospective study was conducted to assess the potential threat posed by processing blood cultures to clinical microbiology laboratory personnel working in an area endemic for brucella melitensis in southern israel. the computerized laboratory records for the 2002-2009 period were reviewed, and the proportion of aerobic vials from which brucella organisms were isolated out of the total number of positive aerobic blood culture vials was determined. during the 8-year period, b. melitensis was i ... | 2011 | 21725863 |
| the genome sequence of brucella pinnipedialis b2/94 sheds light on the evolutionary history of the genus brucella. | abstract: | 2011 | 21745361 |
| an unusual etiology of puo. | infection with brucella spp. continues to pose a human health risk globally despite strides in eradicating the disease from domestic animals. brucellosis has been an emerging disease since the discovery of brucella melitensis by sir david bruce in 1887. although many countries have eradicated b. abortus from cattle, in some areas b. melitensis and b. suis have emerged as causes of this infection in cattle, leading to human infections. currently b. melitensis remains the principal cause of human ... | 2011 | 21751665 |
| hypothermia with pneumonia: a rare presentation of brucellosis. | objective: to report a very rare form of brucellosis presenting with hypothermia and pneumonia. clinical presentation and intervention: a 41-year-old male shepherd presented with a depressed level of consciousness. clinically, his rectal temperature was 29.5°c, and he was cold, apathetic, hyporeflexic, and hypotensive, with atrial fibrillation. he had clinical and radiological evidence of bilateral bronchopneumonia. blood culture and serologic testing were positive for brucella melitensis. the p ... | 2011 | 21757943 |
| an acpxl mutant in rhizobium leguminosarum bv. phaseolus lacks 27-hydroxyoctacosanoic acid in its lipid a and is developmentally delayed during symbiotic infection of the determinate nodulating host plant phaseolus vulgaris. | rhizobium leguminosarum is a gram negative bacterium that forms nitrogen fixing symbioses with compatible leguminous plants via intracellular invasion and establishes a persistent infection within host membrane derived subcellular compartments. notably, an unusual very long chain fatty acid (vlcfa) is found in the lipid a of r. leguminosarum as well as in the lipid a of the medically relevant pathogens brucella abortus, brucella melitensis, bartonella henselae, and legionellae pneumophila which ... | 2011 | 21764936 |