Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| mechanisms of cyclic amp phosphodiesterase regulation. | the mechanisms of regulation of cyclic amp phosphodiesterases were studied using the cytoplasmic fraction of pc-12 cells sensitive to the action of nerve growth factor. the cells contain phosphodiesterases of two types. one of them possesses a high affinity for cyclic amp (km = 2.46 mm), whereas the other has the affinity by an order worse (km = 37.1 mm). pc-12 cell differentiation under the action of nerve growth factor is connected with the cyclic nucleotide elevation; however, activities of b ... | 1983 | 6100586 |
| next year's bee and wasp stings. | 1980 | 6107595 | |
| injectable adrenaline for bee stings. | 1980 | 6107705 | |
| adrenaline for bee-stings. | 1980 | 6107788 | |
| bee venom enhances guanylate cyclase activity. | bee venom and phospholipase a2 extracted from bee venom enhanced guanylate cyclase (e.c. 4.6.1.2) activity two- to threefold in rat liver, lung, heart, kidney, ileum, and cerebellum. dose-response relationships revealed that bee venom at concentrations as low as 1 microgram per milliliter and phospholipase a2 at 1 microunit per milliliter caused a maximal enhancement of guanylate cyclase. | 1981 | 6113689 |
| interactions between initiating chemical carcinogens, tumor promoters, and adenovirus in cell transformation. | cell culture systems that respond to the combined action of initiating chemical carcinogens, tumor promoters, and transforming viruses represent useful model systems for studying the complex multifactor nature of the carcinogenic process. we have utilized both secondary rat embryo (2 degrees re) and a clonal population of established fischer rat embryo (cref) cells to study the effect of multiple agents on the process of adenovirus transformation. in the present review we summarize our investiga ... | 1980 | 6119815 |
| bee-sting diseases: who is at risk? what is the treatment? | many of the large number of people who are stung each year by bees experience frightening systemic reactions, but the vast majority of such reactions are not life-threatening. there is no evidence that the very few who die as a result of a bee sting come from the pool of those who once before sustained a systemic reaction. on the contrary, no reaction at all may be a more ominous predictor of a lethal outcome on a subsequent sting. death comes about through multiple mechanisms, and not through a ... | 1982 | 6121151 |
| bee stings. | 1982 | 6121247 | |
| apamin as a selective blocker of the calcium-dependent potassium channel in neuroblastoma cells: voltage-clamp and biochemical characterization of the toxin receptor. | this paper describes the interaction of apamin, a bee venom neurotoxin, with the mouse neuroblastoma cell membrane. voltage-clamp analyses have shown that apamin at low concentrations specifically blocks the ca2+-dependent k+ channel in differentiated neuroblastoma cells. binding experiments with highly radiolabeled toxin indicate that the dissociation constant of the apamin-receptor complex in differentiated neuroblastoma cells is 15-22 pm and the maximal binding capacity is 12 fmol/mg of prote ... | 1982 | 6122211 |
| proteins in venoms of two wasps, polistes comanchus navajoe and vespa orientalis. | 1. by means of gel electrophoresis the basic proteins in venoms of polistes comanchus navajoe and vespa orientalis were resolved into 6 and 5 proteins respectively, all of molecular weights greater than 15,000. 2. several proteins appeared to be similar in both venoms. 3. the main component of p. comanchus venom responsible for hemolysis was isolated, and data concerning its thermolability, molecular weight (approximately 26,000) and amino acid composition show that unlike the cytolytic componen ... | 1982 | 6122535 |
| bee stings. | 1982 | 6122964 | |
| gila monster and mexican beaded lizard venoms enhance guanylate cyclase activity. | gila monster (heloderma suspectum) and mexican beaded lizard (heloderma horridum) venoms enhanced rat liver, lung, heart, kidney, cerebellar and ileal guanylate cyclase [e.c.4.6.1.2] activity two- to threefold at their 1 microgram/ml concentrations. dose response relationships revealed that the ed50 for stimulation of guanylate cyclase was 10 ng/ml while a concentration of 1 microgram/ml was necessary for the maximal activation with both venoms. varying the concentration of the guanylate cyclase ... | 1982 | 6123143 |
| bee venom hypersensitivity in busselton. | 1982 | 6123766 | |
| the pharmacology of microbracon venom. | 1. wasps of the genus microbracon generally paralyse larvae of lepidoptera. the wasp larva is exophagous on the paralysed host. the venom acts slowly and causes a flaccid paralysis. the venom of m. hebetor is extremely active in lepidoptera, less active in honeybee workers, much less active in locusts and mealworms and probably inactive in non-insects. 2. in lepidoptera and in locusts the venom presynaptically blocks the excitatory, but not the inhibitory, neuromuscular transmission. m. hebetor ... | 1982 | 6128151 |
| effect of apamin on release of vasoactive intestinal polypeptide (vip) from the cat intestines. | the effect of apamin, a polypeptide from bee venom, on the release of vasoactive intestinal polypeptide (vip) during active neurogenic vasodilatation in the intestines was studied in vivo in anesthetized cats. three non-adrenergic, non-cholinergic mechanisms were investigated, i.e. the vasodilatation seen upon transmural electrical field stimulation, pelvic nerve activation and stimulation of the intramural nerves with 5-hydroxytryptamine (5-ht) infused i.a. apamin given close i.a. abolished the ... | 1983 | 6139935 |
| lethal potency in mice of toxins from scorpion, sea anemone, snake and bee venoms following intraperitoneal and intracisternal injection. | the lethal potency of 29 toxins from scorpion, sea anemone, snake and bee venoms was studied. lethality following intracisternal injection of these toxins is considerably higher than following i.p. injection (except for the snake neurotoxins). this is of practical interest in the determination of the concentration of active toxins in a solution when only small amounts are available, as in the case of radiolabelled toxins. | 1984 | 6145236 |
| insect sting anaphylaxis and beta-adrenergic blockade: a relative contraindication. | severe anaphylaxis is reported in a bee venom-sensitive 31-year-old male while receiving propranolol. his anaphylactic reaction was mainly respiratory and was refractory to emergency treatment. we believe that his use of beta-blockers contributed to the severity of his anaphylaxis and refractoriness to treatment. we suggest that beta-blockers should be contraindicated or alternate medications used in hymenoptera allergic patients. | 1984 | 6146275 |
| two species of full-length cdna are synthesized in high yield by melittin-treated avian retrovirus particles. | a method of activating endogenous cdna synthesis in avian retroviruses that results in the formation of two species of full-length cdna in high yield is described. tests of biological activity show infectivity of at least the same order of magnitude as for full-length cdna made by other procedures. melittin, the major component of bee venom, is used as an alternative to nonionic detergents to make the viral envelope permeable and thus activate the endogenous rna-dependent dna polymerase. this co ... | 1980 | 6153806 |
| differential reactivity of isolated mast cells from the rat and guinea pig. | the effects of various chemical histamine liberators on isolated rat peritoneal, rat mesenteric and guinea-pig mesenteric mast cells were examined. all three cell types responded, but to different degrees, to calcium ionophores and surface active agents. the rat mesenteric cells also reponded, but less effectively than the peritoneal cells, to compound 48/80, peptide 401 from bee venom and atp. rat mesenteric cells were essentially refractory to the action of dextran and guinea-pig cells were al ... | 1980 | 6158454 |
| modified basophil degranulation test in diagnosis of bee and wasp sting allergies. | a modified basophil degranulation test proved reliable and easily reproducible in the investigation of 50 patients with allergies to bee and/or wasp stings. its diagnostic value is comparable to that of the rast and it is, in addition, simpler, quicker and cheaper. its disadvantage is that it has to be performed within 24-36 hours after withdrawal of blood. | 1980 | 6160780 |
| alcoholic bouin fixation of insect nervous systems for bodian silver staining. ii. modified solutions. | a previously devised synthetic equivalent of 'aged alcoholic bouin (duboscq-brasil) fixative was modified in various ways to discover which of the chemical changes brought about by aging were important in improving fixation and staining. effects were tested with ventral nerve cord ganglia of the cockroach periplaneta americana, locust schistocerca gregaria, and honey bee apis mellifera. formation of reaction products, chiefly ethyl acetate and diethoxymethane, seemed to play only a subsidiary ro ... | 1980 | 6161444 |
| [effects of melittin and its tetraacetyl derivative on rat liver mitochondria]. | the uncoupling effect of melittin, the principal peptide of bee venom was studied. it was found that the activation of mitochondrial respiration by melittin and its derivative is due to the formation of channels equally penetrable for lithium, sodium, potassium and rubidium ions. the penetrability of the inner mitochondrial membrane appears at melittin or tetraacetyl melittin concentrations about 0.90 or 0.15 nmol/mg of protein, respectively. the appreciable increase of the uncoupling activity o ... | 1980 | 6165401 |
| piracetam activity may differ according to the age of the recipient mouse. | piracetam has bee administered to young and old mice to investigate whether the age may differently modulate the activity of this centrally acting drug. after piracetam administration, old mice showed a notably greater increase of their brain tryptophan than young animals. furthermore, piracetam significantly improves the learning capacity of young mice, but the drug is remarkably more active in improving the performance by old mice. these facts may then suggest that this compound is especially ... | 1980 | 6165698 |
| allergic sensitivity confirmed by the release of histamine from small blood samples. | an enzymatic-isotopic microassay that can measure picogram amounts of histamine in 20 microliter samples of plasma has been used to confirm diagnosis of allergic sensitivity to extracts of house dust mite, cat dander, grass, plantain, cladosporium mould and bee sting venom by the skin test procedure. multiple assays for allergic histamine release can be performed on minimal blood samples resulting in a qualitative and quantitative prediction of allergic sensitivity from an in vitro biochemical p ... | 1981 | 6165940 |
| enzyme-like immunosorbent assay (elisa) for immunoglobulin g antibodies against insect venoms. | igg "blocking" antibodies were measured in patients receiving insect venom immunotherapy. the enzyme-linked immunosorbent assay (elisa) described herein was found to be sensitive and reproducible. results with elisa correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. also, specific antibody titers against phospholipase a and whole bee venom were correlated. serial determinations of venom-specific igg antibodies we ... | 1981 | 6166648 |
| phosphorylation of vesicular stomatitis virus proteins as a possible contributing factor in virion uncoating. | the relationship between the in vitro phosphorylation of vesicular stomatitis virus (vsv) proteins and virion uncoating was examined. activation of the vsv virion kinase with low concentrations of melittin, the active peptide component of bee venom, in the presence of gamma-[32p] atp resulted in the phosphorylation of virion proteins. following the in vitro phosphorylation of vsv proteins in the presence of melittin and deoxyadenosine triphosphate, the virion envelope was disrupted based on the ... | 1981 | 6171611 |
| non-competitive inhibition of honeybee haemolymph pnp-alpha-d-glucosidase by chloramphenicol.--a study in vitro. | the haemolymph pnp-alpha-d-glucosidase activity of emerging worker bees shows a tendency towards negative cooperativity (n = 0.92). the relation between initial velocity and enzyme concentration is not exactly linear (bilogarithmic exponent = 0.91). between 25 degrees and 31 degrees c, the activation energy, ea = 38.2 kj/mol. chloramphenicol administered in vitro decreases the maximum velocity and re-establishes pure michaelian kinetics (n congruent 1.0). the hill coefficient for the binding of ... | 1981 | 6176196 |
| mucosal mast cells. i. isolation and functional characteristics of rat intestinal mast cells. | we have developed a procedure for the dispersion of mast cells from the intestinal lamina propria (lp) and epithelium of rats infected with the intestinal nematode, nippostrongylus brasiliensis. the dispersed cells are morphologically and histochemically similar to intestinal mucosal mast cells (mmc) in situ and are distinguishable from peritoneal mast cells (pmc). mmc derived from the lp or epithelium of parasitized animals secrete histamine in response to the specific parasite antigens as well ... | 1982 | 6176638 |
| mucosal mast cells. ii. effects of anti-allergic compounds on histamine secretion by isolated intestinal mast cells. | functional mast cells have been isolated from the lamina propria of the small intestine of rats infected with the nematode nippostrongylus brasiliensis. the cells released histamine on challenge with specific antigen, anti-rat ige, concanavalin a, and calcium ionophores but were less responsive than peritoneal mast cells (mmc) from the same animals. intestinal mucosa mast cells (pmc) were refractory to the action of the basic secretagogues peptide 401 from bee venom and compound 48/80. the anti- ... | 1982 | 6176639 |
| effect of honeybee (apis mellifera) venom on the course of adjuvant-induced arthritis and depression of drug metabolism in the rat. | the ability of honeybee venom to suppress mycobacterium butyricum-induced arthritis was studied in lewis rats. bee venom, 2 mg.kg-1.day-1 for 24 days, suppressed but did not abolish the primary and secondary inflammatory responses to the adjuvant as monitored by decreases in the swelling of the left and right hind paws and adjuvant-induced arthritis on heme metabolism were also examined. bee venom or adjuvant had no effect on hepatic delta-aminolevulinic acid synthase, porphyrin content, or ferr ... | 1982 | 6177321 |
| studies of chemically modified honeybee venom. i. biochemical, toxicologic and immunologic characterization. | allergens of honeybee venom (bv) were modified by formaldehyde treatment (f), acetoacetylation (a) or coupling to polyethylene glycol (p). the biochemical, toxicologic and immunologic properties of these modified bv preparations were analyzed. f showed strongly reduced cytotoxicity, enzymatic activity and reactivity with human bv-specific ige antibodies but retained the reactivity to bv-specific human and rabbit igg. in a, enzymatic activity, cytotoxicity and the reactivity with human ige and ra ... | 1982 | 6178698 |
| a quantitative enzyme-linked immunosorbent assay for honeybee venom-specific immunoglobulin g. | a quantitative enzyme-linked immunosorbent assay (elisa) for honeybee venom-specific igg is reported. this elisa surmounts the problem of poor reproducibility due to nonparallelism of dilution curves in previously reported elisas. the assay is performed in polyvinyl "u" microtiter plates in which hbv is physically adsorbed to the wells. the antigen is sequentially overlaid with human serum albumin, unknown serum diluted in 10% normal goat serum (ngs), and peroxidase-labeled anti-human igg in ngs ... | 1982 | 6179974 |
| unique functional characteristics of mucosal mast cells. | mast cells have been isolated from the intestine (imc) of rats previously infected with the nematode, nippostrongylus brasiliensis. functional studies on imc have shown that they are responsive to antigen and possess surface ige but, in contrast to peritoneal mast cells (pmc), imc are unresponsive to the basic secretagogues, 48/80 and bee venom peptide 401, and hyporesponsive to ionophores. furthermore, sodium cromoglycate, ah9679 and theophylline inhibited secretion by pmc but not imc, whereas ... | 1982 | 6183933 |
| allergy to honeybee body components: distinction from bee venom sensitivity. | two patients with inhalant allergy to whole bee body components are described, documented by positive skin tests to whole bee body extract and the presence of serum bee body-specific ige. both had evidence of mild sensitivity to bee venom. rast inhibition studies indicated that the ige antibodies directed at whole bee body components and bee venom were distinct. these observations suggest that venom allergy from insect stings and bee body inhalant allergy are caused by different antigens. | 1983 | 6185554 |
| effect of purified phospholipases on glucose transport, insulin binding, and insulin action in isolated rat adipocytes. | the influence of alterations in phospholipid structure by phospholipase treatment on insulin action and glucose transport in rat adipocytes was studied. it appeared that phospholipase a2 from bee venom caused a breakdown of approximately 50% of phosphotidylcholine without lysis of the cells. because of this treatment, insulin binding was increased, resulting in an increased sensitivity of glucose transport towards lower insulin concentrations. moreover, an increased affinity of the transport sys ... | 1982 | 6187461 |
| the use of particle counting immunoassay (pacia) for the titration of specific antibodies. application to sera from honeybee venom-desensitized patients. | particle counting immunoassay (pacia) is shown to be a suitable nonradioisotopic method for the titration of anti-phospholipase a2 antibodies in the sera from honeybee venom-desensitized patients. the sensitivity of the method is equal to the rast-igg with a correlation coefficient between the two methods of r = 0.94. within-day and between-day precisions were 2.5 and 9.2%, respectively. the main advantages of pacia are: (i) the use of easily prepared non-radioactive reagents having a shelf-life ... | 1983 | 6188705 |
| influence of aprotinin and gabexate mesilate on arachidonic acid release by the ca-ionophore a 23187 in the lung. | in a model of isolated, ventilated rabbit lungs, perfused with krebs-henseleit albumin buffer in a recirculating system, increased availability of free aa (arachidonic acid) results in an increase in pulmonary vascular resistance and permeability. the former can be ascribed to cyclooxygenase products of aa, among which thromboxane a2 is mainly responsible. the increase of vascular permeability is, at least partly, due to lipoxygenase products of aa. availability of free aa for the different oxyg ... | 1983 | 6190378 |
| a comparison of histamine secretion from isolated peritoneal mast cells of the mouse and rat. | the effect of different histamine liberators on isolated peritoneal mast cells of the mouse and rat has been examined. both cell types responded to the lectin, concanavalin a, and the release was in each case potentiated by phosphatidylserine. the rat cells released histamine on treatment with dextran but the mouse cells were essentially refractory to the polysaccharide. the mouse cells were significantly more responsive to the actions of the ionophore a23187 and adenosine 5'-triphosphate but mu ... | 1983 | 6194120 |
| diagnosis of polistes wasp hypersensitivity. | patients referred from the houston, texas, metropolitan area were evaluated for allergic reactions to insect stings. forty-eight persons reported at least one systemic reaction caused by a polistes paper-nest wasp sting. honey bees, imported fire ants, and other types of hymenoptera were identified in that order by 19 other subjects with systemic allergic reactions. life-threatening airway obstruction and/or hypotension were noted by most of our patients. wasp venom skin testing was positive in ... | 1983 | 6194197 |
| spiroplasma apis, a new species from the honey-bee apis mellifera. | two spiroplasma strains (b31 and b39) recovered from diseased honey-bees (apis mellifera) in southwestern france were similar in biochemical, serological and pathological properties. the organisms grew at 30 degrees c, required cholesterol for growth, fermented glucose, catabolized arginine and produced a film and spot reaction. the two spiroplasmas were serologically indistinguishable but were related to serogroup iv spiroplasmas, which had been previously isolated from flower surfaces and from ... | 1983 | 6195951 |
| anaphylaxis after hymenoptera stings in three patients with urticaria pigmentosa. | three patients with urticaria pigmentosa are reported who developed symptoms of anaphylaxis after hymenoptera stings. serum ige antibodies to various hymenoptera venoms could not be detected in any of the patients. skin tests were completely negative in one patient, and borderline reactions with honeybee and yellow jacket venom, respectively, were found in the other two. peripheral blood leukocytes of these latter two patients did not release significant amounts of histamine after exposure to th ... | 1983 | 6196389 |
| comparison of the amino acid compositions and antigenic properties of spiralins purified from the plasma membranes of different spiroplasmas. | spiralins were purified by agarose-suspension electrophoresis after extraction with detergents from the membranes of the following spiroplasmas: spiroplasma citri c189, s. citri maroc (r8a2), s. citri scaph and the honey-bee spiroplasma b88. the four proteins (molecular mass congruent to 26,000 daltons, as determined by sodium dodecyl sulphate-pore gradient electrophoresis) showed very similar amino acid compositions characterized by the absence of methionine and tryptophan and a high polarity i ... | 1984 | 6201097 |
| crystallized dextran nanospheres with entrapped antigen and their use as adjuvants. | a novel and simple method is described of preparing a matrix with entrapped antigen, the matrix consisting of well characterized carbohydrates with low toxicity, i.e., dextran and starch, stabilized by crystallization. the crystallized carbohydrate spheres produced probably derive their adjuvant effect from slow antigen release and macrophage stimulation. five months after a single injection of 80 micrograms ovalbumin entrapped in dextran spheres, mice produced antibodies against ovalbumin and t ... | 1984 | 6201561 |
| a comparison of histamine secretion from peritoneal mast cells of the rat and hamster. | functional mast cells have been obtained by peritoneal lavage of the rat and hamster. both cell types released histamine on stimulation with appropriate dilutions of anti-rat ige and anti-hamster serum. the maximum response evoked by each reagent was significantly greater for the hamster cells. the release was non-cytotoxic and was in each case blocked by the corresponding soluble antigen. the rat and hamster cells responded to concanavalin a and the lectin from lentil. phosphatidylserine (ps) p ... | 1984 | 6202354 |
| bee keepers' igg and ige antibody responses to bee venom studied by means of crossed radioimmunoelectrophoresis. | the immune response to honey bee venom in thirty-seven bee keepers' sera was studied by several methods. specific ige antibody levels studied by rast were generally low, whereas specific igg antibody levels studied by a sepharose protein a technique were high. crossed radioimmunoelectrophoresis was applied for a detailed analysis of the antibody specificities towards the different components of venom in seventeen of the bee keepers' sera. significant amounts of igg antibodies were found towards ... | 1984 | 6205790 |
| melittin-specific monoclonal and polyclonal ige and igg1 antibodies from mice. | melittin, a bee venom peptide consisting of 26 amino acid residues, elicited high igg and ige antibody responses in mice of balb/c and caf1 strains, but not in mice of a/j, akr, and c57bl/6 strains. greater than 80% of the melittin-specific antibodies in sera of responder mice were found to bind the hydrophilic carboxyl-terminal heptapeptide of melittin. three melittin-specific monoclonal antibodies were obtained from responder mice by the hybridoma technique. two are of the igg1 isotype and one ... | 1984 | 6207240 |
| [contribution of nmr spectroscopy to the study of structure-function relations of proteins and peptides]. | nmr spectroscopy provides a unique means to study molecular conformation, mechanisms of action and structure-function relationships for peptides and proteins in solution under conditions approaching those of their physiological environment. development of nmr techniques, especially directed to the peptide and protein conformational analysis, is considered under the topics of two-level signal assignment and structural significance of homo- and heteronuclear spin-spin couplings. the results of nmr ... | 1984 | 6210094 |
| studies of chemically modified honeybee venom. ii. immunogenicity and suppression of reaginic antibody formation. | honeybee venom allergens modified by formaldehyde treatment (f), acetoacetylation (a), and coupling to polyethylene glycol (p) were studied with regard to their immunogenicity and their ige-suppressive potential in rabbits and mice. f indiced a strong igg response in rabbits and especially mice, but only a moderate ige response in the mouse. its ige-suppressive potential in mice was comparable to that of unmodified bee venom. a induced only a weak igg and ige response. its ige-suppressive potent ... | 1982 | 6212550 |
| early normalization of platelet survival by endothelial seeding of dacron arterial prostheses in dogs. | current synthetic vascular prostheses do not acquire a complete lining of vascular endothelium in humans or dogs. seeding of autogenous venous endothelium has bee reported to remedy this defect, but previous studies have been primarily morphologic. to see if venous endothelial seeding caused a measurable decrease in platelet-prosthetic interaction in vivo, serial 111in-labeled platelet survival studies were done in dogs with seeded (n = 8) and unseeded (n = 9) thoracoabdominal dacron bypass pros ... | 1982 | 6213055 |
| kinetic study of the inhibition of the honeybee haemolymph apha-glucosidase in vitro by baye 4609, bayg 5421 and bayn 5595. | three therapeutic inhibitors of vertebrate alpha-glucosidases recently assayed in research on diabetes control, show high inhibitory potencies towards the p-np-alpha-d-glucosidase activity of honeybee haemolymph. baye 4609 is an allosteric v-type (pure non-competitive) inhibitor with: ki congruent to k'i congruent to i50 congruent to 180 micro m; n = 1.17; ni = 1.15 bayg 5421, an hydrolysis derivative of the former, is a mixed allosteric inhibitor with: ki congruent to 0.17 micro m; k'i congruen ... | 1982 | 6215920 |
| effects of stimulating the lumbar sympathetic trunk on cat hindlimb muscle spindles. | 1. the effect of stimulating the lumbar sympathetic trunk has been observed on cat lumbrical and tenuissimus muscle spindles. 2. spindle afferent discharges were recorded either from single ia fibers in teased dorsal root filaments or from a large number of spindles by integrating their discharges led from muscles nerves. 3. blood flow in small arteries supplying the muscle was observed through a microscope during and after the stimulation of the sympathetic trunk. 4. in some spindles repetitive ... | 1982 | 6216869 |
| allergy in beekeepers. | beekeepers represent a high-risk group for allergic disorders. they are affected by beestings but only a very limited fraction of the population develops severe anaphylactic symptoms. on the other hand several beekeepers have slight anaphylactic symptoms when they start working. immunologically they are like "spontaneously desensitized subjects". they often have bee-venom specific ige and all of them have very high titers of bee-venom specific igg. beekeepers may present an atopic sensitization ... | 2004 | 6219557 |
| [fatal bee sting: 1st report of igg4-mediated anaphylactic shock]. | the author reports on a 39 year-old male patient with allergic asthma who died several hours after a bee sting in a state of shock. while ige antibodies were not present, distinct deposits of igg4 antibodies in the kidney and lung were demonstrated for the first time after a bee sting. the intention of this report is to stress the importance of hyposensibilisation of patients with an allergic condition after determination of the relevant class or subclass of immunoglobulins. | 1983 | 6224351 |
| occupational dermatitis in a bee-keeper. | 1983 | 6226485 | |
| humoral and cellular immune response of the rat to immunization with bee venom. | lewis rats were immunized with bee venom allergen in freund's complete adjuvant (fca) or with fca only. animals immunized with bee venom developed specific igg antibodies but no specific ige antibodies were detected. lymphocytes from lymph nodes when cultured with antigen in vitro showed an increased stimulation index from day 17 onwards. a concomitant augmentation of t suppressor cells was observed; the t helper/t suppressor cell ratio declined from 4.5:1 before immunization to 1:1 from day 5 o ... | 1984 | 6235990 |
| immunoglobulin e and a levels in atopic and anaphylactic children. | seventy-six children between 18 months and 12 years of age were included in this study. sixty-one were atopic and 15 had anaphylaxis due to bee stings or drugs. immunoglobulins: a, g. m and e were determined and evaluated with respect to age and diagnosis. iga levels were lower in atopic children between six and ten years than in normals. ige levels were high in both atopic and anaphylactic patients but higher in the atopic than in the anaphylactic group. the results are discussed, focusing on t ... | 1980 | 6243451 |
| correlation of igg and ige antibody levels to honey bee venom allergens with protection to sting challenge. | a group of 25 honey bee venom allergic patients were treated with commercial honey bee venom at a monthly maintenance dose of 100 micrograms for approximately one year. at the end of one year 24 patients were intentionally challenged and one was accidentally challenged. three patients experienced significant systemic reactions to challenge and three experienced minor reactions. sera obtained before commencing therapy, at maintenance and before challenge were tested by radioallergosorbent test (r ... | 1981 | 6257151 |
| the use of bee venom melittin to assess the topography of membrane vesicles derived from paracoccus denitrificans. | there exists considerable controversy regarding membrane topography in vesicles derived by osmotic lysis of spheroplasts of gram-negative bacteria. it has been reported by others that bee venom can be used to quantitate the portion of a heterogeneous vesicle population with an inside-out orientation by determining the degree of loss of crypticity of nadh dehydrogenase activity. we have demonstrated that a major component of bee venom, melittin, causes an increase in the activity of several diffe ... | 1980 | 6257350 |
| clearance of extracellular potassium: evidence for spatial buffering by glial cells in the retina of the drone. | work with ion-selective microelectrodes on the retina of the honeybee drone has shown that potassium is released from photoreceptors during activity and enters glial cells. measurements of the extracellular voltage gradients indicate that, in this preparation, currents flowing through the glial cells in the 'spatial buffer' pattern account for a large fraction of the glial k+ entry in the active region of the tissue. | 1981 | 6261870 |
| modification of the tetrodotoxin receptor in electrophorus electricus by phospholipase a2. | the effects of phospholipase a2 treatment on the tetrodotoxin receptors in electrophorus electricus was studied. (1) the binding of [3h]tetrodotoxin to electroplaque membranes was substantially reduced by treatment of the membranes with low concentrations of phospholipase a2 from a number of sources, including bee venom, vipera russelli and crotalus adamanteus and by beta-bungarotoxin. (2) phospholipase a2 from bee venom and from c. adamanteus both caused extensive hydrolysis of electroplaque me ... | 1981 | 6268164 |
| the sting. melittin forms channels in lipid bilayers. | melittin, a toxin of bee venom, is a cationic polypeptide composed of 26 amino acids. the six residues of the c-terminal end are polar and 19 of the 20 residues of the n-terminal end are hydrophobic. exposure of the lecithin bilayer to melittin results in the formation of channels that are more permeable to anions that to cations. unilateral addition of melittin produces a voltage-dependent increase in membrane conductance when the side where the polypeptide is present in made positive but not w ... | 1981 | 6269667 |
| effects of quinine and apamin on the calcium-dependent potassium permeability of mammalian hepatocytes and red cells. | 1. k-sensitive electrodes placed in the extracellular fluid have been used to show that atp and noradrenaline cause a rapid loss of up to 10% of the k content of isolated guinea-pig hepatocytes. 2. the hypothesis tha this response is a consequence of a rise in the k permeability of the hepatocyte membrane triggered by an increase in cytosolic ca is supported by the finding that the divalent cation ionophore a23187 also initiated k loss, in this instance of up to 20-25% of the amount in the cells ... | 1981 | 6273550 |
| calcium and the alpha-action of catecholamines on guinea-pig taenia caeci. | 1. the involvement of calcium in the alpha-action of adrenaline on guinea-pig taenia caeci was studied by measuring the changes in membrane potential and muscle contraction, using the sucrose-gap method, and by determining the (42)k efflux, in the presence of a beta-blocker (propranolol, 1.8 x 10(-6)m).2. in the presence of extracellular calcium, the hyperpolarization caused by adrenaline (3 x 10(-6)m) was sustained during the period of its application (5 min), both in active preparations (at 36 ... | 1981 | 6275064 |
| preparation of a pure monoiodo derivative of the bee venom neurotoxin apamin and its binding properties to rat brain synaptosomes. | the preparation and purification of an active monoiodo derivative of apamin is described. radiolabeled monoiodoapamin (2000 ci/mmol) binds specifically to rat brain synaptosomes at 0 degrees c and ph 7.5 with a second order rate constant of association (ka = 2.6 x 10(7) m-1 s-1) and a first order rate constant of dissociation (kd = 3.8 x 10(-4) s-1). the maximal binding capacity is 12.5 fmol/mg of protein and the dissociation constant is 15-25 pm for the monoiodo derivative and 10 pm for the nat ... | 1982 | 6277913 |
| inactivation and solubilization of opiate receptors by phospholipases a2. | (1) as previously shown, stereospecific binding of opiates to membrane bound receptors is inhibited by treatment with small amounts of phospholipase a2 from vipera russelli. this effect is quantified and compared with the enzymes from the venoms of naja naja siamensis, apis mellifica and from porcine pancreas. all enzymes are equally effective. the inhibition is due to partial phospholipid hydrolysis leading to inactivation of membrane-bound receptor. (2) bee venom phospholipase a2 together with ... | 1982 | 6279149 |
| [effect of serotonin on nervous system function and behavior in the honeybee]. | serotonin in concentration of 0.0025% increased the excitability of chemoreceptors and the dancing rhythm rate in honey bees; higher concentrations diminished electrical activity in the second thoracic ganglion, lowered dancing rhythm rate and accelerated the alteration of food conditioning reflexes. serotonin exerted no effect on the excitability thresholds of the peripheral nervous activity. a possibility of serotoninergic mediation of neural event in disruption of genetically determined trypt ... | 1982 | 6281094 |
| how important is the myeloperoxidase microbicidal system of phagocytic cells? | the myeloperoxidase system is presented by most immunology textbooks as a major microbicidal system of phagocytic cells. this theory, however, has not bee subjected to vigorous testing in the clinical arena. of 14 patients with primary myeloperoxidase deficiency, only 3 had infectious complication. all 3 patients have more plausible explanation than myeloperoxidase deficiency for their infectious complications. two of these patients were healthy until middle age when they developed systemic cand ... | 1982 | 6283326 |
| kinetics of oxygen consumption after a single flash of light in photoreceptors of the drone (apis mellifera). | the time course of the rate of oxygen consumption (qo2) after a single flash of light has been measured in 300-micrometers slices of drone retina at 22 degrees c. to measure delta qo2(t), the change in qo2 from its level in darkness, the transients of the partial pressure of o2 (po2) were recorded with o2 microelectrodes simultaneously in two sites in the slice and delta qo2 was calculated by a computer using fourier transforms. after a 40-ms flash of intense light, delta qo2, reached a peak of ... | 1982 | 6288837 |
| guanine-plus-cytosine content, hybridization percentages, and ecori restriction enzyme profiles of spiroplasmal dna. | the guanine-plus-cytosine (g + c) content of spiroplasmal dna was calculated from the melting temperature determined spectrophotometrically and the buoyant density determined by equilibrium density gradient centrifugation in cscl. only two ranges of g + c values were found: 25-27 mol% and 29-32 mol%. the dna of the following spiroplasmas has 25-27 mol% g + c: spiroplasma citri (serogroup i-1); the spiroplasmas pathogenic to the honeybee (kc3, bc3, and b63; serogroup i-2); the corn stunt strain ( ... | 1982 | 6289407 |
| k+ transport in 'tight' epithelial monolayers of mdck cells. evidence for a calcium-activated k+ channel. | measurements of 86rb efflux across the apical and basal-lateral aspects of intact monolayers of 'high-resistance' mdck cells mounted in ussing chambers have been made. a transient increase in 86rb efflux across both epithelial borders upon stimulation with adrenalineeeeeee or ionophore a23187 is observed. the increased 86rb across the basal cell aspects is of greatest quantitative importance. measurements of total cellular k+ contents by flame photometry of tissue extracts indicate a net loss of ... | 1982 | 6289894 |
| high affinity binding of [125i]monoiodoapamin to isolated guinea-pig hepatocytes. | the bee venom neurotoxin apamin has been labelled with 125i and its binding to isolated guinea-pig hepatocytes measured under physiological conditions. a single saturable component of [125i]monoiodoapamin binding with a kd of 350 pm and bmax of 0.99 fmol/mg dry wt was identified. native apamin displaced labelled apamin with a kd of 376 pm which is broadly in keeping with the concentrations found to inhibit k loss from guinea-pig hepatocytes. these observations, together with the binding found in ... | 1983 | 6298001 |
| guanethidine effects on the guinea pig vas deferens are antagonized by the blockers of calcium-activated potassium conductance, apamin, methylene blue, and quinine. | the blocking effects of guanethidine on electrically induced, neurally mediated, contractions of the guinea pig vas deferens in vitro could be markedly antagonized by the bee venom polypeptide apamin (20-60 nm), by 0.1 mm methylene blue, and (less regularly) by 0.1-0.15 mm quinine, three substances known to inhibit calcium-activated potassium conductance in a variety of cells. guanethidine (20 microm) was also found to inhibit (by 88%) the release of [3h]norepinephrine induced by electrical stim ... | 1983 | 6300650 |
| characterisation of membrane vesicles from paracoccus denitrificans and measurements of the effect of partial uncoupling on their thermodynamics of oxidative phosphorylation. | 1. vesicles from paracoccus denitrificans were prepared by applying an osmotic shock to spheroplasts derived from cells that had been grown anaerobically with succinate as carbon source and nitrate as electron acceptor. in the presence of either phenazinemethosulphate or n,n,n' n',-tetramethyl-p-phenylenediamine, the oxidation of isoascorbate supported the uptake of both s14cn- and 86rb+ (in the presence of valinomycin), whereas nadh and succinate oxidation resulted only in s14cn- uptake. these ... | 1983 | 6301833 |
| fourth-derivative spectrophotometry analysis of tryptophan environment in proteins. application to melittin, cytochrome c and bacteriorhodopsin. | fourth-derivative spectrophotometry is applied to the analysis of solvent effects on the spectral transitions of n-acetyl-l-tryptophan amide, as well as of its mixtures with n-acetyl-l-tyrosine ethyl ester. these compounds were analyzed in different media. it was found that the position of the longest-wavelength minimum of the fourth-derivative spectrum is mainly determined by the nature of the trp environment, with a minor contribution from that of tyr. a geometrical parameter is also defined, ... | 1983 | 6305654 |
| nucleotide sequence of cloned cdna coding for honeybee prepromelittin. | total mrna from venom glands of young queen bees was transcribed into cdna and cloned into the psti site of plasmid pbr322. the nucleotide sequence of two clones with inserts containing genetic information for prepromelittin is presented. the longer insert encompasses 374 base pairs including 52 nucleotides before the initiation codon and a 3' non-coding region of 112 base pairs. the 70 amino acids of prepromelittin represent the total coding capacity of the mrna from which this insert is derive ... | 1983 | 6309516 |
| multiplicity of receptors for vasoactive intestinal polypeptide (vip): differential effects of apamin on binding in brain, uterus and liver. | apamin is a neurotoxic octadecapeptide from bee venom, which has been shown to inhibit the non-adrenergic, non-cholinergic inhibitory innervation of the smooth muscle of the gut. since vasoactive intestinal polypeptide (vip) has been proposed as a possible inhibitory neurotransmitter, the effect of apamin on the receptor binding of 125i-vip was studied using the following assays: (1) isolated synaptosomes from rat cerebral cortex, (2) crude plasma membranes from hog uterine smooth muscle, and (3 ... | 1983 | 6312430 |
| effects of phospholipase a2 on gastric microsomal h+, k+-atpase system: role of "boundary lipids" and the endogenous activator protein. | pig gastric microsomal vesicles enriched in gastric h+,k+-atpase and k+-pnppase were digested with bee venom phospholipase a2 at 21 or 37 degrees c. the unattacked phospholipids were then related to the remaining enzyme activities, followed by reconstitution with microsomal phospholipids and the endogenous activator protein. gastric k+-stimulated atpase was nearly abolished within 10 min of phospholipase a2 treatment. a substantial amount of pnppase activity remained unaffected under identical c ... | 1983 | 6318803 |
| the coexistence in rat muscle cells of two distinct classes of ca2+-dependent k+ channels with different pharmacological properties and different physiological functions. | ca2+-dependent k+ channels responsible for the long-lasting after-hyperpolarization in rat muscle cells in culture are not those extensively studied by the patch-clamp technique. the first ones are blocked by apamin, a bee venom polypeptide, and they are unaffected by tetraethylammonium (tea) whereas the second ones are blocked by tea and unaffected by apamin. these two ca2+-dependent k+ channels coexist in rat muscle cells in culture but also probably in many other cellular types. | 1984 | 6322759 |
| the contribution of calcium and potassium to the alpha-action of adrenaline on smooth muscle cells of the portal vein, pulmonary artery and taenia caeci of the guinea-pig. | the role of calcium and potassium in the alpha-action of adrenaline in pulmonary artery and portal vein was compared with that in taenia caeci by measuring changes in membrane potential, muscle contraction and ion fluxes in quiescent preparations from guinea-pigs (23 degrees c). the depolarization evoked by adrenaline (5 x 10(-8)-3 x 10(-5) m) was sustained in portal vein; in pulmonary artery it declined to a constant level after reaching an initial maximum. in calcium-free medium (20 min) conta ... | 1984 | 6325217 |
| light-induced changes in extracellular volume in the retina of the drone, apis mellifera. | slices of drone retina were superfused with a ringer solution containing 1 mm tetraethylammonium (tea), and the concentration of this ion in the extracellular space [( tea]0) was measured with ion-sensitive microelectrodes. a train of light flashes for 90 s caused [tea] to increase by 48 +/- 4% (s.e.), n = 12. since water crosses cell membranes more readily than tea does this indicates a volume decrease of at least 32%. measurements of ca2+ activity under similar conditions showed an increase of ... | 1984 | 6328378 |
| the ca2+-dependent slow k+ conductance in cultured rat muscle cells: characterization with apamin. | the interaction of apamin, a bee venom neurotoxin, with rat skeletal muscle cell membranes has been followed using both an electrophysiological and a biochemical approach. voltage-clamp analyses have shown that apamin, at low concentrations, specifically blocks the ca2+-dependent slow k+ conductance in rat myotubes and myosacs . a specific binding site for apamin in rat muscle cell membranes has been characterized with the use of a highly radiolabelled apamin derivative [( 125i]apamin). the diss ... | 1982 | 6329722 |
| [experimental rationale and trial of the therapeutic use of bee-raising products in cardiovascular diseases]. | 1983 | 6348365 | |
| [use of bee-raising products in dentistry]. | 2013 | 6353681 | |
| serotonin-immunoreactive neurons in the brain of the honeybee. | the distribution of serotonin-immunoreactive neurons in the brain of the worker honey bee apis mellifera was studied by means of immunocytochemical staining by using a well-characterized antibody to serotonin (5-ht). about 75 immunoreactive perikarya are grouped into clusters in the optic lobe and in the median and dorsal protocerebrum. immunoreactive fibers were resolved in all areas of the brain. the optic lobe shows restricted layers of 5-ht-immunoreactive fibers in the lamina and medulla org ... | 1984 | 6376546 |
| application of the dot immunobinding assay to allergy diagnosis. | the recently developed dot immunobinding assay is operationally simple and facilitates performance of multiple simultaneous assays. here, its use as the basis for determination of total and allergen-specific ige is established. for total ige, the same commercially available polyclonal anti-human ige was used on the solid phase and as a peroxidase conjugate in the liquid phase. after incubation with a chromogenic substrate, ige was determined from the color intensity of the resulting dots with a ... | 1984 | 6376599 |
| shock secondary to massive honeybee toxin. | 2004 | 6381838 | |
| structure and multistep activation of the precursors of peptides from honeybee venom glands and frog skin. | 1984 | 6389022 | |
| spiroplasmas and the transfer of genetic material by transformation and transfection. | two plasmids, pmh1 with 7 kbp and pm41 with 8 kbp were purified from spiroplasma citri strains mh and m4 respectively. on the basis of guanine + cytosine content and restriction enzyme mapping, the two plasmids are different. the linearized pmh1 plasmid was introduced into escherichia coli plasmid vector pbr328 and could be cloned in e. coli. using radioactive probes specific for each plasmid, we found that pm41 was present in three additional s. citri strains and in three other spiroplasmas not ... | 1984 | 6392186 |
| [immunoenzyme method for demonstrating ige and igg antibodies to bee venom]. | 1984 | 6394277 | |
| resistance to apamin of the ca2+-activated k+ permeability in pancreatic b-cells. | the bee venom neurotoxin apamin failed to affect 86rb outflow and insulin release from rat pancreatic islets stimulated by d-glucose or the ca2+-ionophore a23187. apamin, in contrast to quinine or a23187, also failed to affect bioelectrical activity in mouse islet cells. these findings suggest that, like in erythrocytes, and at variance with the situation found in smooth muscle, liver or neuroblastoma cells, the ca2+-activated k+ permeability in the pancreatic b-cell is resistant to apamin. | 1983 | 6411494 |
| postscript to bee stings: delayed 'serum sickness'. | 1983 | 6416995 | |
| changes in membrane phospholipid distribution during platelet activation. | exposure of phospholipids at the outer surface of activated and control platelets was studied by incubation with a mixture of phospholipase a2 from naja naja and bee venom, solely or in combination with sphingomyelinase from staphylococcus aureus, using conditions under which cell lysis remained below 10%. incubation with phospholipase a2 alone revealed a markedly increased susceptibility of the phospholipids in platelets activated by a mixture of collagen plus thrombin, by the sh-oxidizing comp ... | 1983 | 6418205 |
| stimulation of anterior pituitary prolactin release by melittin, an activator of phospholipase a2. | melittin, a 26-amino acid polypeptide contained in bee venom and an activator of phospholipase a2, stimulated prl secretion from bovine anterior pituitary cells in vitro. over the dose range 0.25-2 micrograms/ml, melittin stimulation of prl release was dose-related, reversible, and calcium dependent. within this same dose range melittin did not deplete cell prl stores, nor did it alter [3h]leucine uptake or trypan blue exclusion, indicators of cell viability. the phospholipase a2 inhibitors quin ... | 1984 | 6418521 |
| biogenic amines in the brain of the honeybee, apis mellifera. | fluorescence histochemistry with glyoxylic acid has been used in close conjunction with detailed anatomical studies (mobbs 1982) to investigate the distribution of fluorogenic amines in the brain of the honeybee, apis mellifera. in addition, the concentration and distribution of biogenic amines in the brain of the bee have been determined using highly sensitive radioenzymatic techniques and high performance liquid chromatography. the cerebral ganglia of the bee contain similar amounts of dopamin ... | 1983 | 6420063 |
| total parenteral nutrition in bone marrow transplantation: a clinical evaluation. | bone marrow transplantation (bmt) is associated with severe metabolic stress secondary to anorexia, mucositis, enteritis, and infection. we compared nutritional parameters and clinical outcomes of 22 patients who received prophylactic total parenteral nutrition (tpn) to those of 22 controls, matched for age and diagnosis, who received nutritional support ad libitum. over the 5-week study period, the tpn group averaged caloric intakes greater than 1.5 x basal energy expediture (bee) per day and g ... | 1984 | 6420535 |
| [a stoichiometric interpretation of the significance of the hill coefficient of some kinetically cooperative enzymes. application to alpha-glucosidases of the hemolymph of bees]. | in an enzyme complex where active subunits are aggregated in functional groups undergoing steric interactions, the hill coefficient can reflect the ratio of the partial reaction order of the substrate upon the enzyme one, according to the mass action law. honeybee haemolymph alpha-glucosidases kinetics support this theory in negative and positive cooperativity cases. | 1983 | 6424880 |
| the phospholipid requirement for rho(d) antigen activity: mode of inactivation by phospholipases and of protection by anti-rh0(d) antibody. | previous studies have suggested a membrane phospholipid requirement for rho(d) antigen activity. isolated erythrocyte membranes incubated with phospholipase a2 from both bee venom and porcine pancreas undergo loss of rh antigen activity. the mode of attenuation of this antigen activity as indicated by double-reciprocal binding plots suggests substantial loss of sites accompanied by an apparently increased association constant. in the presence of anti-rho(d), but not anti-a, bound to group a rho( ... | 1984 | 6431264 |
| inhibition of phospholipase a2 in vitro by some anti-hypoxic drugs. | the effect of the anti-hypoxic drugs piracetam, nicergoline, papaverine, cinnarizine and aligeron on the activity of bee venom phospholipase a2 was studied in experiments in vitro. the ph-stat titration method of grossmann et al. was used. all the drugs studied inhibited to a various degree the activity of phospholipase a2 in vitro. the most potent drug was nicergoline and the least potent was papaverine. taking into account that the activity of phospholipase a2 is stimulated in hypoxia, the res ... | 1984 | 6436598 |
| [antipyretic effect of a polypeptide from bee venom--adolapin]. | 1984 | 6440766 | |
| effect of the purified phospholipases a2 from snake and bee venoms on rabbit platelet function. | effects of seven purified phospholipases a2 from the venoms of snakes (naja naja atra, trimeresurus mucrosquamatus and t. gramineus) and honey bee (apis mellifera) on rabbit washed platelet suspension in the absence of bovine serum albumin have been studied. only phospholipases a2 from n. n. atra, t. mucrosquamatus and a. mellifera venoms induced platelet aggregation with small amounts of 14c-serotonin release. they showed tachyphylaxis and also cross-tachyphylaxis in inducing platelet aggregati ... | 1984 | 6441310 |