Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| resistance to cypermethrin and amitraz in rhipicephalus (boophilus) microplus on the santa catarina plateau, brazil. | with the objective of evaluating rhipicephalus (boophilus) microplus resistance to cipermetrina and amitraz, were collected engorged tick females from cattle on 20 farms on the santa catarina plateau, in southern brazil, between january of 2004 and may of 2006. were also received 20 groups of engorged r. (b.) microplus females, collected by cattle farmers requesting acaricidal efficacy (ae) testing. were performed in vitro tests, which consisted of immersing engorged females in cypermethrin (0.0 ... | 2013 | 22832753 |
| ectoparasites of dogs in home environments on the caribbean slope of costa rica. | reports on ectoparasites on dogs in central america are scarce. the aim of this study was to identify flea, louse and tick species infesting dogs in home environments on the caribbean slope of costa rica, and determine their frequency and coexistence. ectoparasites were collected from dogs in 83 rural homes at five study sites. specimens were identified and separated according to species. fleas were the most common ectoparasite (g = 22,217, df = 8, p = 0.004). ctenocephalides felis and pulex sim ... | 2013 | 22832763 |
| genetic parameters of infectious bovine keratoconjunctivitis and its relationship with weight and parasite infestations in australian tropical bos taurus cattle. | infectious bovine keratoconjunctivitis (ibk) or 'pinkeye' is an economically important ocular disease that significantly impacts animal performance. genetic parameters for ibk infection and its genetic and phenotypic correlations with cattle tick counts, number of helminth (unspecified species) eggs per gram of faeces and growth traits in australian tropically adapted bos taurus cattle were estimated. | 2012 | 22839739 |
| virulence potential of metarhizium anisopliae s.l. isolates on rhipicephalus (boophilus) microplus larvae. | the use of entomopathogenic fungi to control arthropods has been reported worldwide for decades. the aim of the present study was to evaluate the virulence of 30 metarhizium anisopliae s.l. brazilian isolates from different geographical regions, hosts or substrates on the larvae of rhipicephalus (boophilus) microplus ticks under in vitro conditions to the selection of virulent isolates in order to be further used in biological control programs. the current study confirmed the lethal action of m. ... | 2012 | 22840642 |
| bioengineered surfaces to improve the blood compatibility of biomaterials through direct thrombin inactivation. | thrombus formation, due to thrombin generation, is a major problem affecting blood-contacting medical devices. this work aimed to develop a new strategy to improve the hemocompatibility of such devices by the immobilization of a naturally occurring thrombin inhibitor into a nanostructured surface. boophilin, a direct thrombin inhibitor from the cattle tick rhipicephalus microplus, was produced as a recombinant protein in pichia pastoris. boophilin was biotinylated and immobilized on biotin-termi ... | 2012 | 22846590 |
| oral vaccination with vaccinia virus expressing the tick antigen subolesin inhibits tick feeding and transmission of borrelia burgdorferi. | immunization with the ixodes scapularis protein, subolesin, has previously been shown to protect hosts against tick infestation and to decrease acquisition of anaplsma marginale and babesia bigemina. here we report the efficacy of subolesin, a conserved tick protein that can act as a regulator of gene expression, expressed from vaccinia virus for use as an orally delivered reservoir - targeted vaccine for prevention of tick infestation and acquisition/transmission of borrelia burgdorferi to its ... | 2012 | 22864146 |
| analysis of babesia bovis infection-induced gene expression changes in larvae from the cattle tick, rhipicephalus (boophilus) microplus. | cattle babesiosis is a tick-borne disease of cattle that has severe economic impact on cattle producers throughout the world's tropical and subtropical countries. the most severe form of the disease is caused by the apicomplexan, babesia bovis, and transmitted to cattle through the bite of infected cattle ticks of the genus rhipicephalus, with the most prevalent species being rhipicephalus (boophilus) microplus. we studied the reaction of the r. microplus larval transcriptome in response to infe ... | 2012 | 22871314 |
| first molecular characterization of anaplasma marginale in cattle and rhipicephalus (boophilus) microplus ticks in cebu, philippines. | anaplasma marginale has been detected in the philippines only by peripheral blood smear examination and serological methods. this study generally aimed to molecularly detect and characterize a. marginale in cattle and ticks in cebu, philippines. a total of 12 bovine blood samples and 60 rhipicephalus (boophilus) microplus ticks were collected on the cebu island in 2011. 16s rrna-based screening-pcr and dna sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for a. marginale, ... | 2013 | 22878542 |
| reinstatement of rhipicephalus (boophilus) australis (acari: ixodidae) with redescription of the adult and larval stages. | rhipicephalus australis fuller, the australian cattle tick, is reinstated and the adults and larvae redescribed from material collected in australia. this long ignored boophilid was previously known as r. microplus canestrini for specimens reported in australia and new caledonia. the adults of r. australis are easily recognized by a combination of characters, such as the ventro-medial spurs in the palpal segments of the male, and the abundant, plumose, pale white setae on the dorsum of the femal ... | 2012 | 22897039 |
| rickettsia sp. strain colombianensi (rickettsiales: rickettsiaceae): a new proposed rickettsia detected in amblyomma dissimile (acari: ixodidae) from iguanas and free-living larvae ticks from vegetation. | from january to december 2009, 55 amblyomma dissimile (koch) ticks removed from iguanas in the municipality of monteria and 3,114 ticks [458 amblyomma sp. larvae, 2,636 rhipicephalus microplus (canestrini) larvae and 20 amblyomma sp. nymphs] collected over vegetation in los cordobas were included in the study. the ticks were pooled into groups from which dna was extracted. for initial screening of rickettsia sp., each pool was analyzed by glta real-time polymerase chain reaction (pcr). positive ... | 2012 | 22897060 |
| characterization and evaluation of a sarcoptes scabiei allergen as a candidate vaccine. | sarcoptic mange caused by the mite sarcoptes scabiei is a worldwide disease affecting both humans and animals. here we report the molecular characterization and evaluation of a recombinant s. scabiei tropomyosin (sstm) protein in a vaccination trial in rabbits. | 2012 | 22897929 |
| abc transporters as a multidrug detoxification mechanism in rhipicephalus (boophilus) microplus. | atp-binding cassette (abc) transporters are responsible for pumping drugs across membranes and are an important drug detoxification mechanism. since abc transporters act on a wide spectrum of chemical compounds, they have been associated with multidrug resistance phenotype in various parasites and cancer cells. here, we document the presence of a rhipicephalus (boophilus) microplus tick population (jaguar) resistant to four acaricide classes (organophosphates (op), synthetic pyrethroids (sp), am ... | 2012 | 22926678 |
| phylogenomic resolution of paleozoic divergences in harvestmen (arachnida, opiliones) via analysis of next-generation transcriptome data. | next-generation sequencing technologies are rapidly transforming molecular systematic studies of non-model animal taxa. the arachnid order opiliones (commonly known as "harvestmen") includes more than 6,400 described species placed into four well-supported lineages (suborders). fossil plus molecular clock evidence indicates that these lineages were diverging in the late silurian to mid-carboniferous, with some fossil harvestmen representing the earliest known land animals. perhaps because of thi ... | 2012 | 22936998 |
| [survey on ticks and detection of new bunyavirus in some vect in the endemic areas of fever, thrombocytopenia and leukopenia syndrome (ftls) in henan province]. | to investigate the distribution, species, seasonal fluctuation of ticks and detect new bunyavirus in some hematophagus in the endemic areas of fever thrombocytopenia and leukopenia syndrome (ftls) in henan province. | 2012 | 22943894 |
| understanding the genetic, demographical and/or ecological processes at play in invasions: lessons from the southern cattle tick rhipicephalus microplus (acari: ixodidae). | the southern cattle tick, rhipicephalus microplus, is the ixodid species causing the largest economic losses in tropical agrosystems because of its recurrent invasive success, explosive demography on bovine herds, vector competence for diverse pathogens and frequent development of acaricide resistance. its ecology and the physiological bases of the acaricide resistances it developed, as well as alternative tick control measures, have been intensively studied for decades. by contrast, the tick po ... | 2013 | 22945880 |
| acaricidal action of destruxins produced by a marine-derived beauveria felina on the bovine tick rhipicephalus (boophilus) microplus. | the increasing resistance of rhipicephalus (boophilus) microplus tick to commercial insecticides requires alternative methods for the control of this cattle plague. the enthomopathogenic fungus beauveria felina produces destruxins in culture media, cyclic depsipeptides which display an array of biological activities. the present investigation aimed to evaluate the acaricide action of destruxins isolated from b. felina culture media on r. (b.) microplus engorged females. b. felina was grown in mf ... | 2012 | 22955115 |
| protective immunity against tick infestation in cattle vaccinated with recombinant trypsin inhibitor of rhipicephalus microplus. | the cattle tick, rhipicephalus microplus, is regarded as the most economically important ectoparasite of livestock globally. control is achieved primarily through the use of acaricides. this approach is hampered by the development of resistance to commercial acaricides among cattle tick populations. vaccination against r. microplus infestation is another technology that can be integrated for effective cattle tick control. proteins belonging to the kunitz-bpti family are abundant in cattle tick s ... | 2012 | 22959980 |
| tick control practices in burkina faso and acaricide resistance survey in rhipicephalus (boophilus) geigyi (acari: ixodidae). | traditional systems account for 95 % of the livestock produced in burkina faso. tick infestation hampers livestock productivity in this area. however, little information exists on tick-control practices used by livestock farmers. we interviewed 60 stockbreeders working in traditional farming systems to obtain the first data on tick-control practices. sixteen farmers (27 %) did not use conventional practices: seven removed ticks by hand or plastered cattle with dung or engine oil; nine farmers tr ... | 2013 | 22968469 |
| multi-antigenic vaccine against the cattle tick rhipicephalus (boophilus) microplus: a field evaluation. | the tick rhipicephalus (boophilus) microplus is a blood-sucking ectoparasite of cattle that severely impairs livestock production. studies on tick immunological control address mostly single-antigen vaccines. however, from the commercial standpoint, so far no single-antigen vaccine has afforded appropriate protection against all r. microplus populations. in this context, multi-antigen cocktails have emerged as a way to enhance vaccine efficacy. in this work, a multi-antigenic vaccine against r. ... | 2012 | 22981764 |
| chemical composition and acaricidal activity of essential oil from lippia sidoides on larvae of dermacentor nitens (acari: ixodidae) and larvae and engorged females of rhipicephalus microplus (acari: ixodidae). | the aim of this work was to identify the compounds and to investigate the acaricidal activity of the essential oil from the leaves of lippia sidoides on rhipicephalus microplus and dermacentor nitens. the oil was obtained by hydrodistillation and analyzed by gas chromatography (gc/fid) and gas chromatography/mass spectrometry. in total, 15 compounds comprising 99.97 % of the total peak area were identified. the main constituent of the essential oil was thymol (67.60 %). the acaricidal activity w ... | 2012 | 22983219 |
| primary embryonic cells of rhipicephalus microplus and amblyomma cajennense ticks as a substrate for the development of borrelia burgdorferi (strain g39/40). | borrelia burgdorferi, the agent of lyme borreliosis, is a spirochetes transmitted by ticks to humans and animals. its cultivation in vitro in tick cells allows studies of its biology and provides methodology for future research in brazil, and for the isolation of borrelia spp. we examined in vitro the characteristics of embryonic cells of rhipicephalus microplus and amblyomma cajennense in cell culture and investigated the suitability of embryonic cells as a substrate for cultivation of b. burgd ... | 2012 | 22990829 |
| direct tests of enzymatic heme degradation by the malaria parasite plasmodium falciparum. | malaria parasites generate vast quantities of heme during blood stage infection via hemoglobin digestion and limited de novo biosynthesis, but it remains unclear if parasites metabolize heme for utilization or disposal. recent in vitro experiments with a heme oxygenase (ho)-like protein from plasmodium falciparum suggested that parasites may enzymatically degrade some heme to the canonical ho product, biliverdin (bv), or its downstream metabolite, bilirubin (br). to directly test for bv and br p ... | 2012 | 22992734 |
| cloning and sequence analysis of chitin synthase gene fragments of demodex mites. | to our knowledge, few reports on demodex studied at the molecular level are available at present. in this study our group, for the first time, cloned, sequenced and analyzed the chitin synthase (chs) gene fragments of demodex folliculorum, demodex brevis, and demodex canis (three isolates from each species) from xi'an china, by designing specific primers based on the only partial sequence of the chs gene of d. canis from japan, retrieved from genbank. results show that amplification was successf ... | 0 | 23024043 |
| first report of ctenocephalides felis felis infestation of buffalo calves in punjab, india. | two female buffalo calves of approximately 3 months of age from hoshiarpur and gurdaspur districts of punjab state, india were found to be moderately infested with the cat flea ctenocephalides felis felis. the calves were apparently healthy with no clinical signs and skin lesions. the calves were also found to be simultaneously infested with ixodid tick (rhipicephalus (boophilus) microplus). infestations of buffalo calves by c. felis felis is reported for the first time. | 2011 | 23024513 |
| first report of fipronil resistance in rhipicephalus (boophilus) microplus of mexico. | five strains of rhipicephalus (boophilus) microplus collected from tamaulipas mexico were tested for resistance against several classes of acaricides commonly used. all were resistant to fipronil. four of five were co-resistant to permethrin and coumaphos in addition to being resistant to fipronil. one strain, el zamora was found multi-resistant to permethrin, coumaphos, fipronil, and amitraz. selection with fipronil for 3 generations produced a resistance ratio of 8.3 and 9.4 at the lc(50) and ... | 2013 | 23026557 |
| pyrosequencing-based analysis of the microbiome associated with the horn fly, haematobia irritans. | the horn fly, haematobia irritans, is one of the most economically important pests of cattle. insecticides have been a major element of horn fly management programs. growing concerns with insecticide resistance, insecticide residues on farm products, and non-availability of new generation insecticides, are serious issues for the livestock industry. alternative horn fly control methods offer the promise to decrease the use of insecticides and reduce the amount of insecticide residues on livestock ... | 2012 | 23028533 |
| acetylcholinesterases of blood-feeding flies and ticks. | acetylcholinesterase (ache) is the biochemical target of organophosphate (op) and carbamate pesticides for invertebrates, vertebrate nerve agents, and ache inhibitors used to reduce effects of alzheimer's disease. organophosphate pesticides (ops) are widely used to control blood-feeding arthropods, including biting flies and ticks. however, resistance to ops in pests affecting animal and human health has compromised control efficacy. op resistance often results from mutations producing an op-ins ... | 2013 | 23036311 |
| efficacy of hyalomma scupense (hd86) antigen against hyalomma excavatum and h. scupense tick infestations in cattle. | the rhipicephalus microplus recombinant bm86-based tick vaccines have shown their efficacy for the control of several hyalomma cattle ticks genera, namely h. dromedarii and h. anatolicum. however, h. scupense species, the most important tick in north africa has never been studied. vaccination trials using either a recombinant bm86-based vaccine or a recombinant hd86-based vaccine (the bm86 ortholog in h. scupense) were conducted in cattle against immature and adult h. scupense ticks and adult h. ... | 2012 | 23036501 |
| novel and simple approach using synthesized nickel nanoparticles to control blood-sucking parasites. | the present study was on assessment of the anti-parasitic activities of nickel nanoparticles (ni nps) against the larvae of cattle ticks rhipicephalus (boophilus) microplus and hyalomma anatolicum (a.) anatolicum (acari: ixodidae), fourth instar larvae of anopheles subpictus, culex quinquefasciatus and culex gelidus (diptera: culicidae). the metallic ni nps were synthesized by polyol process from ni-hydrazine as precursor and tween 80 as both the medium and the stabilizing reagent. the synthesiz ... | 2013 | 23040768 |
| bioinformatics and expression analyses of the ixodes scapularis tick cystatin family. | the cystatins are inhibitors of papain- and legumain-like cysteine proteinases, classified in merops subfamilies i25a-i25c. this study shows that 84 % (42/50) of tick cystatins are putatively extracellular in subfamily i25b and the rest are putatively intracellular in subfamily i25a. on the neighbor joining phylogeny guide tree, subfamily i25a members cluster together, while subfamily i25b cystatins segregate among prostriata or metastriata ticks. two ixodes scapularis cystatins, aay66864 and is ... | 2012 | 23053911 |
| use of the larval tarsal test to determine acaricide resistance in rhipicephalus (boophilus) microplus brazilian field populations. | acaricide resistance of the cattle tick rhipicephalus (boophilus) microplus is widespread in most of the countries where this parasite is present. bioassays are used to diagnose the level and pattern of resistance in tick populations. in the present study, we describe a detailed protocol of the larval tarsal test (ltt) using simplified equipment and data on the resistance of 17 tick field populations originating from 5 brazilian states. nine acaricidal compounds from 5 major classes were tested: ... | 2013 | 23059136 |
| principal component analysis on the perceptions of milk producers about rhipicephalus (boophilus) microplus control in minas gerais. | milk producers in lavras, passos and divinópolis, minas gerais, were interviewed with the aim of evaluating their perceptions and attitudes regarding control over rhipicephalus (boophilus) microplus. multivariate correlation between the variables was done by means of principal component analysis. the producers' perceptions and attitudes regarding r. (b.) microplus were similar: most of them did not have any basic knowledge of tick biology or control, and they applied acaricide products through b ... | 2012 | 23070431 |
| bovine immunoprotection against rhipicephalus (boophilus) microplus with recombinant bm86-campo grande antigen. | the southern cattle fever tick, rhipicephalus (boophilus) microplus, is no doubt the most economically important ectoparasite of cattle globally. the inappropriate use of chemical acaricides has driven the evolution of resistance in populations of r. (b.) microplus. anti-tick vaccines represent a technology that can be combined with acaricides in integrated control programs to mitigate the impact of r. (b.) microplus. the recombinant form of bm86 antigen from the campo grande (rbm86-cg) strain o ... | 2012 | 23070436 |
| ticks on buffaloes (bubalus bubalis) in the state of rio de janeiro, brazil. | some tick species have been described parasitizing buffaloes, but reports from brazil are rare. this study aimed to ascertain the species of ticks that parasitize buffaloes in the state of rio de janeiro. seventeen farm properties were visited between june 2008 and december 2009, and buffaloes were inspected in relation to infestation. the ticks were identified and a semi-structured questionnaire on parasitism and treatment against ticks was filled out through interviews with the owners. the spe ... | 2012 | 23070448 |
| detection of anti-borrelia burgdorferi antibodies in buffaloes (bubalus bubalis) in the state of pará, brazil. | this study aimed to investigate the frequency of homologous antibodies of igg class against borrelia burgdorferi in buffaloes in the state of pará, brazil. blood serum samples from 491 buffaloes were analyzed by means of the indirect elisa test, using crude antigen produced from a cultivar of the north american strain g39/40 of b. burgdorferi. there were 412 positive samples (83.91%), and there was no statistically significant difference in the proportions of positive animals between the 81.69% ... | 2012 | 23070455 |
| [cloning and expression of triosephosphate isomerase gene of boophilus microplus]. | total rna was extracted from adult boophilus microplus. rt-pcr was used to amplify the gene and the fragment was subcloned into the expression vector pet-28a. the cloned gene was expressed in e. coli rosseta (de3), induced by iptg, and identified by sds-page. the results showed that the triosephosphate isomerase (tim) gene of b. microplus has 750bp and encodes 249 amino acids (genbank no. jx112888). the cloned tim gene shares 99% homology with that in tick embryos. the relative molecular weight ... | 2012 | 23072171 |
| tick-box for 3'-end formation of mitochondrial transcripts in ixodida, basal chelicerates and drosophila. | according to the trna punctuation model, the mitochondrial genome (mtdna) of mammals and arthropods is transcribed as large polycistronic precursors that are maturated by endonucleolytic cleavage at trna borders and rna polyadenylation. starting from the newly sequenced mtdna of ixodes ricinus and using a combination of mitogenomics and transcriptional analyses, we found that in all currently-sequenced tick lineages (prostriata, metastriata and argasidae) the 3'-end of the polyadenylated nad1 an ... | 2012 | 23077630 |
| acaricidal properties of ricinus communis leaf extracts against organophosphate and pyrethroids resistant rhipicephalus (boophilus) microplus. | indian cattle ticks have developed resistance to commonly used acaricides and an attempt has been made to formulate an ecofriendly herbal preparation for the control of acaricide resistant ticks. a 95% ethanolic extract of ricinus communis was used to test the efficacy against reference acaricide resistant lines by in vitro assay. in in vitro assay, the extract significantly affects the mortality rate of ticks in dose-dependent manner ranging from 35.0 ± 5.0 to 95.0 ± 5.0% with an additional eff ... | 2013 | 23084038 |
| frequency distribution of hard ticks (acari: ixodidae) infesting bubaline population of district toba tek singh, punjab, pakistan. | the current research was conducted to define the epidemiological parameters related to the prevalence and associated risk factors of tick infestation in buffaloes in the toba tek singh district of central punjab, pakistan. the prevalence of ticks on buffaloes was 31.21 % (352/1,128). among the species of ticks, the prevalence of hyalomma marginatum (75.56 %; 266/352) was higher (p < 0.05; odd's ratios (or) = 3.09) than rhipicephalus microplus (24.44 %; 86/352). female buffaloes (69.60 %; 245/352 ... | 2013 | 23086441 |
| survey of pyrethroids resistance in indian isolates of rhipicephalus (boophilus) microplus: identification of c190a mutation in the domain ii of the para-sodium channel gene. | monitoring acaricide resistance and understanding the underlying mechanisms are critically important in developing strategies for resistance management and tick control. eighteen isolates of rhipicephalus (boophilus) microplus collected from four agro-climatic regions of india were characterized and the resistant data were correlated with bioassay results, esterase enzyme activities and with the presence/absence of point mutation in the para-sodium channel gene. the adult immersion test was stan ... | 2013 | 23092687 |
| comparative genomic analysis and phylogenetic position of theileria equi. | transmission of arthropod-borne apicomplexan parasites that cause disease and result in death or persistent infection represents a major challenge to global human and animal health. first described in 1901 as piroplasma equi, this re-emergent apicomplexan parasite was renamed babesia equi and subsequently theileria equi, reflecting an uncertain taxonomy. understanding mechanisms by which apicomplexan parasites evade immune or chemotherapeutic elimination is required for development of effective ... | 2012 | 23137308 |
| neutral lipid composition changes in the fat bodies of engorged females rhipicephalus microplus ticks in response to fungal infections. | the tick's fat body plays an essential role in energy storage and utilization. this study aimed to analyze the fat body neutral lipid composition in rhipicephalus microplus engorged females. in the first study (physiological profile of untreated ticks), the lipid analysis took place over the course of 4 days; the engorged females were incubated at optimal conditions and their fat bodies were dissected daily. fat body lipid analysis after fungal infection with metarhizium anisopliae sensu lato (s ... | 2013 | 23138474 |
| tick cell culture isolation and growth of rickettsia raoultii from dutch dermacentor reticulatus ticks. | tick cell lines play an important role in research on ticks and tick-borne pathogenic and symbiotic microorganisms. in an attempt to derive continuous dermacentor reticulatus cell lines, embryo-derived primary cell cultures were set up from eggs laid by field ticks originally collected as unfed adults in the netherlands and maintained for up to 16 months. after several months, it became evident that cells in the primary cultures were infected with a rickettsia-like intracellular organism. supern ... | 2012 | 23140894 |
| molecular and pharmacological characterization of serotonin 5-ht2α and 5-ht7 receptors in the salivary glands of the blowfly calliphora vicina. | secretion in blowfly (calliphora vicina) salivary glands is stimulated by the biogenic amine serotonin (5-hydroxytryptamine, 5-ht), which activates both inositol 1,4,5-trisphosphate (insp(3))/ca(2+) and cyclic adenosine 3',5'-monophosphate (camp) signalling pathways in the secretory cells. in order to characterize the signal-inducing 5-ht receptors, we cloned two cdnas (cv5-ht2α, cv5-ht7) that share high similarity with mammalian 5-ht(2) and 5-ht(7) receptor genes, respectively. rt-pcr demonstra ... | 2012 | 23145175 |
| in vitro diagnosis of the first case of amitraz resistance in rhipicephalus microplus in santo tomé (corrientes), argentina. | in argentina, the cattle tick rhipicephalus microplus has already developed resistance to organophosphates and synthetic pyrethroids. however, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. a recent failure of amitraz to control ticks in a farm located in santo tomé, province of corrientes, resulted in the collection of samples for acaricide resistance diagnosis. the modified drummond adult immersion test (ait) and the larval tarsal test (ltt) were ... | 2013 | 23153823 |
| chemical composition and biological activities of essential oil from hyptis crenata growing in the brazilian cerrado. | essential oils from species of the genus hyptis are well-known for their significant biological properties, including antimicrobial and acaricidal activities. the essential oil from the aerial parts of h. crenata was obtained by hydrodistillation; bomeol (17.8%), 1,8-cineol (15.6%) and p-cimene (7.9%) were characterized by gc-ms as its major constituents. the essential oil was evaluated in vitro for its antimicrobial activities against six fungal and five bacterial strains, by measuring the resp ... | 2012 | 23157018 |
| tick infestations in extensively grazed cattle and efficacy trial of high-cis cypermethrin pour-on preparation for control of ticks in mvomero district in tanzania. | this study aimed at determining the extent of tick infestations in extensively grazed cattle and assess the efficacy of ecotix® acaricide (2.5% high cis cypermethrin) in mvomero district in tanzania. a total of 1200 tanzanian short horn zebu (tshz) from two farms in two villages were qualitatively assessed for tick infestations and 40 animals (grouped in 10s from each farm) were separated in their herds and quantitatively examined to establish the tick load. the animals were grouped in treatment ... | 2012 | 23164198 |
| eco-friendly approach using marine actinobacteria and its compounds to control ticks and mosquitoes. | ticks and mosquitoes are ectoparasitic arthropods that can transmit a variety of diseases to humans and animals during blood feeding and causing serious infectious disorders. the purpose of the present study was to assess the acaricidal and insecticidal property of ethyl acetate extract and its compounds isolated from marine actinobacteria, streptomyces vitstk7 sp. against the larvae of cattle ticks, haemaphysalis bispinosa and rhipicephalus (boophilus) microplus (acari: ixodidae); fourth-instar ... | 2013 | 23180127 |
| theileria, babesia, and anaplasma detected by pcr in ruminant herds at bié province, angola. | distribution of anaplasma spp., babesia spp., theileria spp., and ehrlichia ruminantium, was for the first time studied in bié province, central angola. we examined 76 blood samples of cattle originated from seven farms, and 13 blood samples of goats from two farms employing molecular genetic tools (pcr). most prevalent was a. ovis-infection in goats (100%) and a. marginale-infection in cattle (38% of examined animals, and six out of seven farms). b. bigemina-infection was detected in only one s ... | 0 | 23193527 |
| chemical composition and efficacy of dichloromethane extract of croton sphaerogynus baill. (euphorbiaceae) against the cattle tick rhipicephalus microplus (acari: ixodidae). | the cattle tick rhipicephalus microplus is widely distributed in tropical and subtropical regions, causing high economic impact on cattle production. the control of tick infestations is regarded worldwide as critical and has been based on the use of organophosphates, synthetic pyretroids, amitraz and recently ivermectin and fipronil. the present study reports the analysis by gas chromatography/mass spectrometry of the constituents of leaf extracts of croton sphaerogynus and results of acaricidal ... | 2013 | 23200750 |
| inhibition of enzyme activity of rhipicephalus (boophilus) microplus triosephosphate isomerase and bme26 cell growth by monoclonal antibodies. | in the present work, we produced two monoclonal antibodies (brbm37 and brbm38) and tested their action against the triosephosphate isomerase of rhipicephalus (boophilus) microplus (rmtim). these antibodies recognize epitopes on both the native and recombinant forms of the protein. rrmtim inhibition by brbm37 was up to 85% whereas that of brbrm38 was 98%, depending on the antibody-enzyme ratio. rmtim activity was lower in ovarian, gut, and fat body tissue extracts treated with brbm37 or brb ... | 2012 | 23202941 |
| cloning and characterization of a p2x receptor expressed in the central nervous system of lymnaea stagnalis. | p2x receptors are membrane ion channels gated by extracellular atp. mammals possess seven distinct p2x subtypes (p2x1-7) that have important functions in a wide array of physiological processes including roles in the central nervous system (cns) where they have been linked to modulation of neurotransmitter release. we report here the cloning and functional characterization of a p2x receptor from the mollusc lymnaea stagnalis. this model organism has a relatively simple cns consisting of large re ... | 2012 | 23209755 |
| immune response of bos indicus cattle against the anti-tick antigen bm91 derived from local rhipicephalus (boophilus) microplus ticks and its effect on tick reproduction under natural infestation. | antigens of anti-tick vaccines are more efficacious for homologous challenge with local tick strains. cdna clones encoding for bm91 from local rhipicephalus (boophilus) microplus strains were developed to immunize bos indicus cattle under field conditions. three groups of six animals each were injected with the antigen bm91, saline, and adjuvant, respectively. animals were immunized three times at 3-week intervals and a fourth time after six months. the anti-bm91 antibody level, measured by elis ... | 2012 | 23213489 |
| partial selective treatment of rhipicephalus microplus and breed resistance variation in beef cows in rio grande do sul, brazil. | rhipicephalus microplus infestation causes heavy losses in cattle. the majority of farmers control the cattle tick by using long-acting drugs throughout the year, which evidently increases selection pressure for drug resistance. partial selective treatment (pst) may be used to directly reduce selection pressure by treating only the portion of the herd that is most infested. the objective of this work was to determine the effect of pst in two herds of beef cows and the effect of breed tolerance. ... | 2013 | 23219046 |
| a nuclear single-nucleotide polymorphism (snp) potentially useful for the separation of rhodnius prolixus from members of the rhodnius robustus cryptic species complex (hemiptera: reduviidae). | the design and application of rational strategies that rely on accurate species identification are pivotal for effective vector control. when morphological identification of the target vector species is impractical, the use of molecular markers is required. here we describe a non-coding, single-copy nuclear dna fragment that contains a single-nucleotide polymorphism (snp) with the potential to distinguish the important domestic chagas disease vector, rhodnius prolixus, from members of the four s ... | 2012 | 23219914 |
| evolution of the tissue factor pathway inhibitor-like kunitz domain-containing protein family in rhipicephalus microplus. | one of the principle mechanisms utilised by ticks to obtain a blood meal is the subversion of the host's haemostatic response. this is achieved through the secretion of saliva containing anti-haemostatic proteins into the feeding lesion. lineage-specific expansion of predicted secretory protein families have been observed in all previously studied ticks and occurred in response to adaptation to a blood-feeding environment. of these, the predominant families are common between both hard and soft ... | 2013 | 23220044 |
| glutamate decarboxylase of the parasitic arthropods ctenocephalides felis and rhipicephalus microplus: gene identification, cloning, expression, assay development, identification of inhibitors by high throughput screening and comparison with the orthologs from drosophila melanogaster and mouse. | glutamate decarboxylase (l-glutamate 1-carboxylyase, e.c. 4.1.1.15, gad) is the rate-limiting enzyme for the production of γ-aminobutyric acid (gaba), the major inhibitory neurotransmitter in vertebrates and invertebrates. we report the identification, isolation and characterization of cdnas encoding gad from the parasitic arthropods ctenocephalides felis (cat flea) and rhipicephalus microplus (cattle tick). expression of the parasite gad genes and the corresponding drosophila melanogaster (frui ... | 2013 | 23220582 |
| activity of essential oil of lippia triplinervis gardner (verbenaceae) on rhipicephalus microplus (acari: ixodidae). | the objective of this work was to characterize and investigate the acaricidal activity of the essential oil of the aerial parts of lippia triplinervis at different concentrations on unengorged larvae and engorged females of rhipicephalus microplus. the essential oil yielded 2.21 % (w/w to dry matter) and was composed mainly of carvacrol (31.9 %), thymol (30.6 %), and p-cymene (12.3 %). two tests were performed to assess the acaricidal activity: the modified larval packet test, with concentration ... | 2013 | 23224609 |
| new species of ehrlichia isolated from rhipicephalus (boophilus) microplus shows an ortholog of the e. canis major immunogenic glycoprotein gp36 with a new sequence of tandem repeats. | ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses that inflict serious and fatal infections in companion animals and livestock. the aim of this paper was to phylogeneticaly characterise a new species of ehrlichia isolated from rhipicephalus (boophilus) microplus from minas gerais, brazil. | 2012 | 23231731 |
| influence of the physiological state on infestation by rhipicephalus microplus in dairy cows. | the purpose of this study was to identify the influence of physiological state, season, breed, number of lactations, dairy productivity, and stocking rate on infestation by rhipicephalus microplus in dairy cows. two hundred cows were selected through proportional representative sampling at the seropédica experimental station (pesagro-rio), brazil. fully or partially engorged r. microplus females measuring between 4.5 and 8.0mm were counted on the right side of each animal. the tick infestation p ... | 2013 | 23238247 |
| acaricidal activity of ethanolic extract from aerial parts of tagetes patula l. (asteraceae) against larvae and engorged adult females of rhipicephalus sanguineus (latreille, 1806). | the tick rhipicephalus sanguineus is the species with the largest worldwide distribution and is proven to be involved in the transmission of pathogens such as babesia canis, ehrlichia canis, coxiella burnetii, rickettsia ricketsii, rickettsia conorii, among others. studies have demonstrated acquisition of resistance to some of the active principles used in commercial formulations of acaricides. tagetes patula (asteraceae) is a plant with highlighted economic and commercial importance due to the ... | 2012 | 23244493 |
| borrelia burgdorferi and tick proteins supporting pathogen persistence in the vector. | borrelia burgdorferi, a pathogen transmitted by ixodes ticks, is responsible for a prevalent illness known as lyme disease, and a vaccine for human use is unavailable. recently, genome sequences of several b. burgdorferi strains and ixodes scapularis ticks have been determined. in addition, remarkable progress has been made in developing molecular genetic tools to study the pathogen and vector, including their intricate relationship. these developments are helping unravel the mechanisms by which ... | 2013 | 23252492 |
| the conserved role of the akt/gsk3 axis in cell survival and glycogen metabolism in rhipicephalus (boophilus) microplus embryo tick cell line bme26. | tick embryogenesis is a metabolically intensive process developed under tightly controlled conditions and whose components are poorly understood. | 2013 | 23274741 |
| characterization of the uukuniemi virus group (phlebovirus: bunyaviridae): evidence for seven distinct species. | evolutionary insights into the phleboviruses are limited because of an imprecise classification scheme based on partial nucleotide sequences and scattered antigenic relationships. in this report, the serologic and phylogenetic relationships of the uukuniemi group viruses and their relationships with other recently characterized tick-borne phleboviruses are described using full-length genome sequences. we propose that the viruses currently included in the uukuniemi virus group be assigned to five ... | 2013 | 23283959 |
| molecular detection of acinetobacter species in lice and keds of domestic animals in oromia regional state, ethiopia. | this study was conducted to determine the presence of acinetobacter and rickettsia species dna in lice and melophagus ovinus (sheep ked) of animals from oromia regional state in ethiopia. from september through november 2011, a total of 207 cattle, 85 sheep, 47 dogs and 16 cats were examined for ectoparasites. results of morphological identification revealed several species of ectoparasites: linognathus vituli (l. vituli), bovicola bovis (b. bovis) and solenopotes capillatus (s. capillatus) on c ... | 2012 | 23285015 |
| analysis of immunogenic relevant proteins in rhipicephalus (boophilus) annulatus tick. | considering the importance of ticks as a main group transmitting pathogen organisms, this study designed to recognize immunogenic proteins in different tissues of rhipicephalus (boophilus) annulatus tick and to find out if there are common proteins in these tissues. | 2012 | 23293777 |
| chemical identification of tagetes minuta linnaeus (asteraceae) essential oil and its acaricidal effect on ticks. | the control of tick species that affect animal production is vital for the economic welfare of the cattle industry. this study focused on testing the acaricidal activity of the essential oil from the leaves and stems of tagetes minuta against several brazilian tick species, including rhipicephalus (boophilus) microplus, rhipicephalus sanguineus, amblyomma cajennense and argas miniatus. the chemical composition of the essential oil was determined by chromatography and spectroscopy analyses, which ... | 2013 | 23295821 |
| a novel approach, based on blsoms (batch learning self-organizing maps), to the microbiome analysis of ticks. | ticks transmit a variety of viral, bacterial and protozoal pathogens, which are often zoonotic. the aim of this study was to identify diverse tick microbiomes, which may contain as-yet unidentified pathogens, using a metagenomic approach. dna prepared from bacteria/archaea-enriched fractions obtained from seven tick species, namely amblyomma testudinarium, amblyomma variegatum, haemaphysalis formosensis, haemaphysalis longicornis, ixodes ovatus, ixodes persulcatus and ixodes ricinus, was subject ... | 2013 | 23303373 |
| meningoencephalitis from borrelia miyamotoi in an immunocompromised patient. | ixodes ticks serve as vectors for borrelia burgdorferi, the agent of lyme disease. globally, these ticks often concurrently harbor b. miyamotoi, a spirochete that is classified within the relapsing-fever group of spirochetes. although humans presumably are exposed to b. miyamotoi, there are limited data suggesting disease attributable to it. we report a case of progressive mental deterioration in an older, immunocompromised patient, and even though koch's postulates were not met, we posit b. miy ... | 2013 | 23323900 |
| ticks on pastures and on two breeds of cattle in the eastern cape province, south africa. | many studies on the population dynamics of questing ticks on pastures and of parasitic ticks on cattle have been conducted. few, however, have attempted to link the two in a single study. this study aimed to assess the population dynamics of questing ixodid ticks on pastures and of adult ticks on two breeds of cattle with different levels of susceptibility to tick infestation on the same pastures. between january 2005 and december 2009 questing ixodid ticks were collected monthly from natural pa ... | 2011 | 23327215 |
| epidemiological perspectives of ticks and tick-borne diseases in south sudan: cross-sectional survey results. | a cross-sectional study was conducted between september and october 2010 in five states of south sudan that were selected on the basis of the perceived risk of tick-borne diseases. the purpose was to investigate epidemiological parameters of tick-borne diseases in south sudan and their uses in future control strategies. a total of 805 calves were assessed by clinical, microscopic and serological examination and tick counts. the indirect enzyme-linked immuno-sorbent assay (elisa) was used to dete ... | 2012 | 23327317 |
| acaricidal activity of lippia gracilis essential oil and its major constituents on the tick rhipicephalus (boophilus) microplus. | the present study aimed to evaluate the activity of lippia gracilis schauer essential oil obtained from different l. gracilis genotypes and their major components, carvacrol and thymol against rhipicephalus (boophilus) microplus (cattle tick) larvae and engorged females. the larval test was performed parallel to the adult immersion test for engorged females for four l. gracilis genotypes. similar tests were further performed for their major compounds carvacrol and thymol. carvacrol (lc50 of 0.22 ... | 2013 | 23337330 |
| in vitro activities of plant extracts from the brazilian cerrado and pantanal against rhipicephalus (boophilus) microplus (acari: ixodidae). | a total of 73 ethanol extracts from different anatomical parts of 44 plant species belonging to 24 families, native to the mid-western region of brazil, were assessed in vitro for their effect on the reproductive cycle of engorged females of the cattle tick rhipicephalus (boophilus) microplus, using the adult immersion test. all extracts were evaluated at the concentration of 0.2 % and, among the extracts tested, the one obtained from the fruits of guarea kunthiana (meliaceae) proved to be highl ... | 2013 | 23344640 |
| effect of aqueous extracts of baccharis trimera on development and hatching of rhipicephalus microplus (acaridae) eggs. | this study evaluated the effects of aqueous extracts of baccharis trimera (less.) dc (asteraceae), colloquially known as carqueja, on egg production, and hatching rate of larvae of rhipicephalus microplus. plant samples were collected in montes claros, north of minas gerais, brazil. adult female ticks were distributed into 24 homogeneous groups of 10. the in vitro test was performed by immersing each group in 10 ml solutions of aqueous extracts at 50, 100, 150, or 200mg of fresh leaves ml(-1). t ... | 2013 | 23347758 |
| effect of new ethyl and methyl carbamates on biological parameters and reproduction of the cattle tick rhipicephalus microplus. | the effect of carbamates on engorged female rhipicephalus microplus ticks and larvae was evaluated using the adult immersion test (ait) and the larval packet test (lpt), respectively. seventeen synthetic carbamates different from current commercial acaricides were synthesised at the national autonomous university of mexico. none of the carbamates had an effect on the percentage of females laying eggs. six of the compounds inhibited egg laying up to 65.4% and inhibited egg hatching by up to 100% ... | 2013 | 23347759 |
| assessment of the acaricidal activity of carvacrol, (e)-cinnamaldehyde, trans-anethole, and linalool on larvae of rhipicephalus microplus and dermacentor nitens (acari: ixodidae). | the acaricidal activity of carvacrol, (e)-cinnamaldehyde, trans-anethole, and linalool was studied on rhipicephalus microplus and dermacentor nitens larvae. all the substances were tested at concentrations of 2.5, 5.0, 10.0, 15.0, and 20.0 μl/ml, with 10 repetitions per treatment. the modified larval packet technique was employed in the tests and the mortality was evaluated after 24 h. in the groups treated with carvacrol, the lowest concentration (2.5 μl/ml) was sufficient to cause 100% death o ... | 2013 | 23354938 |
| the effects of ingested mammalian blood factors on vector arthropod immunity and physiology. | the blood feeding behavior of disease-transmitting arthropods creates a unique intersection between vertebrate and invertebrate physiology. here, we review host blood-derived factors that persist through blood digestion to affect the lifespan, reproduction, and immune responses of some of the most common arthropod vectors of human disease. | 2013 | 23370408 |
| acaricidal activity of essential oils from five endemic conifers of new caledonia on the cattle tick rhipicephalus (boophilus) microplus. | the aim of the present study was to demonstrate acaricidal activity on the cattle tick rhipicephalus (boophilus) microplus of essential oils from endemic conifers of new caledonia in the context of the development of natural alternatives. acaricidal activity of essential oils extracted from resin and heartwood of five endemic conifers of new caledonia (araucaria columnaris, agathis moorei, agathis ovata, callitris sulcata, and neocallitropsis pancheri) was evaluated on 14- to 21-day-old rhipicep ... | 2013 | 23371495 |
| a steroidal molecule present in the egg wax of the tick rhipicephalus (boophilus) microplus inhibits bacterial biofilms. | the cattle tick rhipicephalus (boophilus) microplus lays eggs in the soil near the roots of grass, or in similar highly moist environments that are prone to biofilm formation. tick eggs have a protective wax coating that may be a source of nutrients for microorganisms. however, as the eggs remain viable and show no visible signs of microbial colonization, we hypothesized that the coating might have anti-biofilm properties. we show here that the coating inhibits biofilm formation by both gram-neg ... | 2013 | 23419060 |
| red deer (cervus elaphus) as a host for the cattle tick rhipicephalus microplus (acari: ixodidae) in yucatan, mexico. | rhipicephalus microplus is the most economically important cattle tick in the mexican tropics. wild ungulate species, including red deer (cervus elaphus), are gaining popularity in diversified livestock ranching operations in mexico. however, there is no information available on the susceptibility of red deer to infestation with the cattle tick, r. microplus, under hot, subhumid tropical conditions in mexico. biological data on r. microplus as an ectoparasite of cattle and red deer in a farm in ... | 2013 | 23423423 |
| ontology for vector surveillance and management. | ontologies, which are made up by standardized and defined controlled vocabulary terms and their interrelationships, are comprehensive and readily searchable repositories for knowledge in a given domain. the open biomedical ontologies (obo) foundry was initiated in 2001 with the aims of becoming an "umbrella" for life-science ontologies and promoting the use of ontology development best practices. a software application (obo-edit; *.obo file format) was developed to facilitate ontology developmen ... | 2013 | 23427646 |
| deorphanization and target validation of cross-tick species conserved novel amblyomma americanum tick saliva protein. | we previously identified a cross-tick species conserved tick feeding stimuli responsive amblyomma americanum (aam) av422 gene. this study demonstrates that aamav422 belongs to a novel group of arthropod proteins that is characterized by 14 cysteine amino acid residues: c(23)-x7/9-c(33)-x23/24-c(58)-x8-c(67)-x7-c(75)-x23-c(99)-x15-c(115)-x10-c(126)-x24/25/33-c(150)c(151)-x7-c(159)-x8-c(168)-x23/24-c(192)-x9/10-c(202) predicted to form seven disulfide bonds. we show that aamav422 protein is a ubiq ... | 2013 | 23428900 |
| cattle tick-associated bacteria exert anti-biofilm and anti-tritrichomonas foetus activities. | research on microbiota in cattle tick and the evaluation of its activity against other microorganisms can contribute to identify new molecules potentially useful to control infections caused by bacteria and protozoa. biofilms pose increasing problems worldwide, mainly due to their resistance to antimicrobial therapies and host immune response. in this study we investigate the ability rhipicephalus (boophilus) microplus-associated bacteria may exhibit to produce anti-biofilm and trichomonicidal c ... | 2013 | 23434012 |
| evolutionary and molecular analysis of the emergent severe fever with thrombocytopenia syndrome virus. | in 2009, a novel bunyavirus, called severe fever with thrombocytopenia syndrome virus (sftsv) was identified in the vicinity of huaiyangshan, china. clinical symptoms of this zoonotic virus included severe fever, thrombocytopenia, and leukocytopenia, with a mortality rate of ~10%. by the end of 2011 the disease associated with this pathogen had been reported from eleven chinese provinces and human-to-human transmission suspected. however, current understanding of the evolution and molecular epid ... | 2012 | 23438426 |
| why so many unknown genes? partitioning orphans from a representative transcriptome of the lone star tick amblyomma americanum. | genomic resources within the phylum arthropoda are largely limited to the true insects but are beginning to include unexplored subphyla, such as the crustacea and chelicerata. investigations of these understudied taxa uncover high frequencies of orphan genes, which lack detectable sequence homology to genes in pre-existing databases. the ticks (acari: chelicerata) are one such understudied taxon for which genomic resources are urgently needed. ticks are obligate blood-feeders that vector major d ... | 2013 | 23445305 |
| cutaneous hypersensitivity responses to rhipicephalus tick larval antigens in pre-sensitized cattle. | nguni cattle are known to be more resistant to ticks than bonsmara cattle, even if the immunological mechanisms responsible for this phenomenon are not fully understood. cutaneous hypersensitivity responses to unfed larval extracts (ule) of the ticks rhipicephalus decoloratus and rhipicephalus microplus were investigated in nguni and bonsmara cattle to improve knowledge on the immunity to ticks. hypersensitivity reactions were induced by intradermal inoculation of 0.1ml of ule of r. decoloratus ... | 2013 | 23453577 |
| efficacy of strychnos spinosa (lam.) and solanum incanum l. aqueous fruit extracts against cattle ticks. | the efficacy of solanum incanum and strychnos spinosa aqueous fruit extracts was evaluated against cattle ticks in on-station experiments and laboratory tick bioassays. in the on-station experiment using cattle, fruit extracts were applied at three concentrations 5, 10, and 20 % (w/v) and compared with a commercial acaricide, tickbuster (amitraz) spray (positive control) and no treatment (negative control). the treatments were applied at weekly intervals for 6 weeks as surface sprays on 32 masho ... | 2013 | 23456785 |
| ticks on birds in a savanna (cerrado) reserve on the outskirts of uberlândia, minas gerais, brazil. | we report tick infestations on birds, in the environment and on domestic animals in a non-forested phytophysiognomy, the savanna-like cerrado sensu stricto, in a natural reserve on the outskirts of the urban area of uberlândia, minas gerais, brazil. overall, 238 birds within 50 species, 15 families and six orders were caught. passeriformes were the most numerous, with 216 birds (90.75%), among which 22 had ticks (n = 31). within this order, the prevalence of tick infestation was 10.2%, and the a ... | 2013 | 23471429 |
| molecular epidemiology of theileria equi in horses and their association with possible tick vectors in the state of rio de janeiro, brazil. | the aim of this study was to detect theileria equi (laveran 1901) dna in horses and ticks using real-time pcr and to list the factors associated with infection in animals located in the seropedica and petropolis municipalities of the state of rio de janeiro. we tested blood samples from 314 horses and samples from 300 ticks, including 191 amblyomma cajennense, 104 dermacentor nitens, and 5 ixodida larvae. factors inherent to the horse, the ownership, and animal management were obtained from an e ... | 2013 | 23474658 |
| association of bovine toll-like receptor 4 with tick infestation rates and blood histamine concentration. | we investigated a possible association between bovine toll-like receptor 4 (tlr4) and resistance to tick infestation in 103 cattle, including bmy cattle (1/2 brahman, 1/4 murray grey, and 1/4 yunnan yellow cattle), brahman, and red angus grazing on improved pasture. the tick infestation weight and number of rhipicephalus microplus and the blood histamine concentration were measured and compared with those of 32 chinese holsteins and 30 simmentals. a 228-bp fragment was amplified and sequenced to ... | 2013 | 23479166 |
| in vitro ovicidal and cestocidal effects of toxins from bacillus thuringiensis on the canine and human parasite dipylidium caninum. | bacillus thuringiensis is a gram-positive soil-dwelling bacterium that is commonly used as a biological pesticide. this bacterium may also be used for biological control of helminth parasites in domestic animals. in this study, we evaluated the possible ovicidal and cestocidal effects of a total protein extract of b. thuringiensis native strains on the zoonotic cestode parasite of dogs, dipylidium caninum (d. caninum). dose and time response curves were determined by coincubating b. thuringiensi ... | 2012 | 23484087 |
| detection of rickettsia parkeri from within piura, peru, and the first reported presence of candidatus rickettsia andeanae in the tick rhipicephalus sanguineus. | domestic farm animals (n=145) were sampled for the presence of ectoparasites in northwestern peru during march, 2008. ninety domestic animals (62%) were positive for the presence of an ectoparasite(s) and produced a total collection of the following: 728 ticks [amblyomma maculatum, anocentor nitens, rhipicephalus (boophilus) microplus, rhipicephalus sanguineus, and otobius megnini], 12 lice (haematopinus suis), and 3 fleas (ctenocephalides felis). a rickettsia genus-specific qpcr assay was perfo ... | 2013 | 23488453 |
| gene expression profiling of adult female tissues in feeding rhipicephalus microplus cattle ticks. | the southern cattle tick, rhipicephalus microplus, is an economically important pest, especially for resource-poor countries, both as a highly adaptive invasive species and prominent vector of disease. the increasing prevalence of resistance to chemical acaricides and variable efficacy of current tick vaccine candidates highlight the need for more effective control methods. in the absence of a fully annotated genome, the wealth of available expressed sequence tag sequence data for this species p ... | 2013 | 23500075 |
| regionalization of oviducts in boophilus microplus (canestrini, 1887) (acari: ixodidae) and its potential significance for fertilization. | the structural analysis of oviducts in boophilus microplus (canestrini, 1887) in different stages of ingurgitation has indicated that they are constituted primarily of an internal cylinder and an external cylinder with different cell types being found between them. copulated females in active ingurgitation process show typical variations along the internal cylinder, and three regions can be defined: anterior, ring-like and transitional. based on such regionalization, hypotheses were raised about ... | 1999 | 23505653 |
| seasonal and spatial distribution of ixodid tick species feeding on naturally infested dogs from eastern austria and the influence of acaricides/repellents on these parameters. | effective control of tick infestation and pathogen transmission requires profound knowledge of tick biology in view of their vector function. the particular time of the year when the different tick species start to quest and the favoured sites on the canine host are of major interest. the efficacy of acaricides/repellents to control ticks in the field requires observation. | 2013 | 23510263 |
| amblyomma americanum tick saliva serine protease inhibitor 6 is a cross-class inhibitor of serine proteases and papain-like cysteine proteases that delays plasma clotting and inhibits platelet aggregation. | we previously demonstrated that amblyomma americanum tick serine protease inhibitor 6 (aams6) was secreted into the host during tick feeding and that both its mrna and protein were ubiquitously and highly expressed during the first 3 days of tick feeding. this study demonstrates that aams6 is a cross-class inhibitor of both serine- and papain-like cysteine proteases that has apparent antihaemostatic functions. consistent with the typical inhibitory serpin characteristics, enzyme kinetics analyse ... | 2013 | 23521000 |
| vaccine delivery using nanoparticles. | vaccination has had a major impact on the control of infectious diseases. however, there are still many infectious diseases for which the development of an effective vaccine has been elusive. in many cases the failure to devise vaccines is a consequence of the inability of vaccine candidates to evoke appropriate immune responses. this is especially true where cellular immunity is required for protective immunity and this problem is compounded by the move toward devising sub-unit vaccines. over t ... | 2013 | 23532930 |
| molecular survey of hard ticks in endemic areas of tick-borne diseases in china. | over the past several years, there was a substantial increase in the number of cases of known and novel tick-borne infections in humans in china. to better understand the ticks associated with these infections, we collected hard ticks from animals or around livestock shelters in 29 localities in 5 provinces (beijing, henan, hubei, inner mongolia, and zhejiang) where cases of tick-borne illness were reported. we collected 2950 hard ticks representing 7 species of 4 genera (dermacentor sinicus, ha ... | 2013 | 23538111 |
| acaricides efficiency on rhipicephalus (boophilus) microplus from bahia state north-central region. | the cattle tick rhipicephalus (boophilus) microplus is responsible for major losses in the brazilian livestock, mainly due to reduction in cattle productive performance. resistance development to major classes of acaricide widely used nowadays has been extensively reported, as well as the occurrence of residues from these compounds in animal products and the environment. this study aimed to evaluate the efficiency of acaricides on r. (b.) microplus collected from rural properties in the north-ce ... | 2013 | 23538503 |
| determination of acaricide resistance in rhipicephalus (boophilus) microplus (acari: ixodidae) field populations of argentina, south africa, and australia with the larval tarsal test. | infestations with ticks have an important economic impact on the cattle industry worldwide and resistance to acaricides has become a widespread phenomenon. to optimize their treatment strategy, farmers need to know if and against which classes potential acaricide-resistance does occur. bioassays are used to assess the resistance level and pattern of rhipicephalus (boophilus) microplus populations. the objective of the current study was to assess the susceptibility of field populations originatin ... | 2013 | 23540121 |