Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antifungal susceptibility of epigallocatechin 3-o-gallate (egcg) on clinical isolates of pathogenic yeasts. | this is the first report to investigate the antifungal susceptibility of 21 clinical isolates of seven candida species to epigallocatechin 3-o-gallate (egcg) and to compare with six antifungal agents, amphotericin b (amph), fluconazole (flcz), flucytosin (5fc), itraconazole (itcz), micafungin (mcfg), and miconazole (mcz), using a method following the national committee for clinical laboratory standards (nccls) m27-a guidelines. among the tested species, candida glabrata exhibited the highest sus ... | 2006 | 16831406 |
| candida-host cell receptor-ligand interactions. | the interaction of candida species with their cognate host receptors is a key factor in the pathogenesis of different types of candidiasis. the recognition of different forms of candida albicans by toll-like receptors 2 and 4 on mononuclear leukocytes has recently been discovered to determine the function and activity of regulatory t-cells, determine the balance of type 1 and type 2 cytokines and, thereby, influence the antifungal activity of both the innate and adaptive immune response. differe ... | 2006 | 16837237 |
| [in vitro resistance to fluconazole and itraconazole in clinical isolates of candida spp and cryptococcus neoformans]. | an in vitro susceptibility testing of 181 strains of six species of candida and 21 strains of cryptococcus neoformans was carried out in order to investigate the resistance to new antifungal drugs. we have studied clinical isolates from 200 different patients of hospital del mar (barcelona) and hospital la inmaculada (almería). an agar diffusion method (neosensitabs, rosco, taastrup, denmark), was employed with fluconazole, itraconazole, and reference drugs amphotericin b, flucytosine, tioconazo ... | 1997 | 16854170 |
| distribution and epidemiology of candida species causing fungemia at a saudi arabian hospital, 1996-2004. | the objective of this study was to evaluate the candida species and the change over time in the organisms causing candidemia at saudi aramco medical services organization in saudi arabia. we also describe the risk factors associated with mortality. | 2007 | 16859945 |
| antimicrobial activity of extracts of the lichen xanthoparmelia pokornyi and its gyrophoric and stenosporic acid constituents. | the antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. both the extracts and the acids showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocol ... | 2006 | 16869486 |
| candida albicans and candida glabrata clinical isolates exhibiting reduced echinocandin susceptibility. | a recognized hotspot for mutations conferring reduced echinocandin susceptibility (res) is residue s645 of candida albicans gsc1(fks1). we report that the mutation f641y is associated with res in a c. albicans isolate. the analogous fks2 residue is mutated f to v in a candida glabrata res isolate; the introduction of this mutation into susceptible c. glabrata confirmed its role in res. y641-equivalent fks residues were identified in intrinsically res fusarium species and candida guilliermondii. | 2006 | 16870797 |
| [pathogenic fungi with increasing proliferation]. | this paper compared occurrence and intenseness of growth c. glabrata, s. capsularis and t. beigelii isolated from sputum, material's bronchoscopic, oral cavity, skin and catheters during 10 years observation (1991 - 2000). all investigated fungi from 1997 years have been characteristic very distinct rise expansiveness appeared in more intenseness of growth and growing rate of replace new ontocenoses. most likely is this consequence successive of decrease immunity of man organism about infections ... | 2001 | 16886398 |
| [the comparison of susceptibility of candida albicans and candida glabrata to miconazole]. | candida glabrata is very important pathogen which is resistant to some imidazoles (fluconazole). the aim of study was to compare a susceptibility of candida albicans and candida glabrata - strains isolated from patietnts treated in centre for treatment of parasitic diseases and mycoses - to miconazole using mic. 96.5% of strains were sensitive to miconazole; values of mic for c. albicans were lower than for c. glabrata. | 2001 | 16886440 |
| [biodiversity of mycocoenoses of oral cavity, throat and nose of children aged 6-15 years]. | the purpose of this study was analysis of biodiversity of mycoflora in selected ontocenoses of healthy children. the material for the study was consisted of swabs taken from oral cavity, pharynx, and nose of healthy children: 128 girls and 142 boys. the material for the study was collected in may and november, dividing the children into two age groups: 6-9 years and 10-15 years. a total of 13 species representing 5 genera: candida, rhodotorula, saccharomyces, saccharomycopsis and trichosporon we ... | 2003 | 16888934 |
| effects of yeast on automated cell counting. | we studied the effects of yeast in peripheral blood samples on results reported by an advia 120/2120 hematology system (bayer healthcare, diagnostics division, tarrytown, ny). in a simulated candidemia model, very high concentrations (1-5 x 10(8) colony-forming units [cfu]/ml) of candida glabrata and candida parapsilosis caused a spuriously elevated platelet count. no such effect was observed with candida albicans. all 3 yeast species, when present at a concentration of 1-5 x 10(6) cfu/ml or gre ... | 2006 | 16891201 |
| azole resistance of candida glabrata in a case of recurrent fungemia. | we describe a case of recurrent candida glabrata fungemia that became unresponsive to fluconazole treatment. posttreatment isolates from blood and vaginal cultures of the immunocompetent patient were azole resistant and exhibited upregulated expression of cgcdr1/cgcdr2 efflux pumps compared to the original isolates. amphotericin b therapy eradicated the infection. | 2006 | 16891541 |
| limitations of caspofungin in the treatment of obstructive pyonephrosis due to candida glabrata infection. | caspofungin is a new antifungal agent with high-level activity against a number of candida species including those that are resistant to azoles. its good safety profile and low nephrotoxicity makes it an attractive drug to treat fungal infections in patients with compromised renal function. however, little is known about the clinical efficacy in the treatment of complicated urinary tract infections due to candida species such as pyonephrosis. | 2006 | 16895593 |
| fluconazole and itraconazole resistance of yeasts isolated from the bloodstream and catheters of hospitalized pediatric patients. | in critically ill children, candida species and other yeasts appear as an important nosocomial pathogen. the emerging fungal pathogens are usually less susceptible to azole compounds, and the management of such infections could be problematic. | 2006 | 16899974 |
| how long do nosocomial pathogens persist on inanimate surfaces? a systematic review. | inanimate surfaces have often been described as the source for outbreaks of nosocomial infections. the aim of this review is to summarize data on the persistence of different nosocomial pathogens on inanimate surfaces. | 2006 | 16914034 |
| comparative study of disc diffusion and microdilution methods in susceptibility testing of micafungin against candida species. | to compare the clinical laboratory standards institute clsi m44-a disc diffusion (dd) and m27-a2 broth microdilution (md) methods for determining the susceptibility of candida spp. to micafungin (fk463). | 2006 | 16916868 |
| synthesis and antifungal activity of new thienyl and aryl conazoles. | recent studies reported that an first generation azole (tioconazole) was active against candida glabrata petite mutants, a fluconazole- and voriconazole- resistant strain of fungi characterized as most azole resistant yeast by an overexpression of the efflux pumps. therefore, monosubstituted 1-[2-(2,4-dichlorophenyl)ethyl]-1h-imidazoles differing from tioconazole by the nature of the linker and of the aromatic ring in their side-chain were synthesized and evaluated against the mutant and the wil ... | 2006 | 16918077 |
| antimicrobial effect, in vitro, of gutta-percha points containing root canal medications against yeasts and enterococcus faecalis. | the objective of this study was to determine the in vitro susceptibility of enterococcus faecalis and the most prevalent candida species as therapy-resistant microorganisms to gutta-percha points containing root canal medications. | 2006 | 16920552 |
| [occurrence of the yeast and filamentous fungi in haematological patients during 2002 to august 2005]. | the number of diseases caused by yeast and fungi has been increasing recently. the primary disease is caused by dermatophytes and cryptococcus neoformans. other types of yeast and fungi can cause secondary infections of patients with predisposition factors. among the mycotic diseases diagnosed in our laboratory can be included candidosis, cryptococcosis, aspergillosis and mucormycosis. | 2006 | 16921787 |
| onychomycosis in primary school children: association with socioeconomic conditions. | onychomycosis in childhood is reported to be unusual. the aim of this study was to determine the prevalence of onychomycosis in primary school children and to make comparison between different socioeconomic status in the rural and urban areas of the city. hand and foot nails of 23235 children aged 7-14 were examined. onychomycosis was suspected and nail scrapings for mycological examination were taken in 116 of them. hyphae or spores were seen in 41 (0.18%) by direct microscopic examination, and ... | 2006 | 16922798 |
| different consequences of ace2 and swi5 gene disruptions for virulence of pathogenic and nonpathogenic yeasts. | mutants of candida albicans, candida glabrata, and saccharomyces cerevisiae with disruptions in the ace2 gene and c. glabrata and s. cerevisiae swi5 disruption mutants were tested for virulence in a murine challenge model of disseminated yeast infection. all mutants showed a clumping phenotype, but clumping was minimized in challenge inocula by inclusion of chitinase in the growth medium. in animals rendered temporarily neutropenic by cyclophosphamide treatment, the c. glabrata ace2 null mutant ... | 2006 | 16926418 |
| survey of amphotericin b susceptibility of candida clinical isolates determined by etest. | the minimal inhibitory concentrations (mics) of amphotericin b (amb) determined by the national committee for clinical laboratory standards (nccls; nccls document m27-a) broth dilution method are in a relatively narrow ranges and this may lead to underestimation of the amb-resistant rate in clinical isolates. we evaluated in vitro susceptibility of clinical isolates of candida spp. to amb using etest and determined the distribution of amb mics in different species. | 2006 | 16926981 |
| in vitro pharmacodynamics of anidulafungin and caspofungin against candida glabrata isolates, including strains with decreased caspofungin susceptibility. | the activities of anidulafungin and caspofungin against candida glabrata were evaluated. mics, 50% inhibitory concentrations (ic(50) values), and ic(90) values for anidulafungin were lower than those for caspofungin for 16 of 18 strains tested. anidulafungin has potent in vitro activity against c. glabrata that is maintained against isolates with elevated caspofungin mics. | 2006 | 16940061 |
| a one-enzyme pcr-rflp assay for identification of six medically important candida species. | early identification of candida isolates to the species level is necessary for effective antifungal therapy, and can also facilitate control of hospital infections. phenotype-based methods for identifying candida species are often difficult and time-consuming. molecular biological techniques provide a useful alternative approach. in the present study, the its1-5.8s-its2 regions of fungal rrna genes were amplified with universal primers in 20 standard strains. digestion of the pcr products with o ... | 2006 | 16940958 |
| accumulation of cadmium ions in the methylotrophic yeast hansenula polymorpha. | intracellular cadmium (cd(2+)) ion accumulation and the ability to produce specific cd(2+) ion chelators was studied in the methylotrophic yeast hansenula polymorpha. only one type of cd(2+) intracellular chelators, glutathione (gsh), was identified, which suggests that sequestration of this heavy metal in h. polymorpha occurs similarly to that found in saccharomyces cerevisiae, but different to schizosaccharomys pombe and candida glabrata which both synthesize phytochelatins. cd(2+) ion uptake ... | 2006 | 16946986 |
| genital candida species detected in samples from women in melbourne, australia, before and after treatment with antibiotics. | vulvovaginal candidiasis (vvc) remains a common cause of morbidity, with three-quarters of women affected during their lifetimes. use of antibiotics is an acknowledged trigger for vvc, which adversely affects women's physical and emotional health. knowledge of patterns of genital candida species-level identification is important for management, as candida species other than candida albicans often fail first-line treatment. a community sample of women with no vaginal symptoms, and who were prescr ... | 2006 | 16954250 |
| anidulafungin pharmacokinetics and microbial response in neutropenic mice with disseminated candidiasis. | candidemia is often fatal, especially in patients with persistent neutropenia. new therapies are needed. we performed 24-h pharmacodynamic studies to compare the efficacies of anidulafungin, fluconazole, and amphotericin b in neutropenic mice with disseminated candidiasis caused by one of three strains of candida glabrata. anidulafungin produced a maximal fungal kill (e(max)) of 1.4 to 1.9 log(10) cfu/g in kidneys and was not influenced by resistance to either fluconazole or amphotericin b. fluc ... | 2006 | 16954319 |
| measurement of serum d-arabinitol/creatinine ratios for initial diagnosis and for predicting outcome in an unselected, population-based sample of patients with candida fungemia. | d-arabinitol (da) is a useful diagnostic marker for candidiasis in patients with neutropenia and other high-risk groups, but its use in unselected patients with a broad range of underlying diseases and conditions has not been studied. we used an automated enzymatic fluorometric assay to measure serum da/creatinine ratios (da/cr's) in 30 healthy adults, 100 hospitalized controls without candida fungemia, and 83 patients from a study of all candida fungemias in connecticut between october 1998 and ... | 2006 | 16957030 |
| antifungal efficacy and pharmacodynamics of posaconazole in experimental models of invasive fungal infections. | posaconazole is a novel lipophilic antifungal triazole with potent and broad-spectrum activity against opportunistic, endemic and dermatophytic fungi. this activity extends to organisms that are often refractory to existing triazoles, amphotericin b or echinocandins such as candida glabrata, candida krusei, aspergillus terreus, fusarium spp. and the zygomycetes. a large number of experimental animal models of invasive fungal infections has demonstrated the potent and broad-spectrum efficacy of p ... | 2006 | 16961576 |
| significant increase of glycolytic flux in torulopsis glabrata by inhibition of oxidative phosphorylation. | this study was aimed at increasing the glycolytic flux of the multivitamin-auxotrophic yeast torulopsis glabrata by disturbing oxidative phosphorylation. we examined two different strategies to impede oxidative phosphorylation. the first strategy was disruption of the activity of the electron transfer chain (etc), by either of two approaches. one was separately adding, at 10 mg l1, specific inhibitors of complex i (rotenone) or of the bc1 complex (antimycin a) to the culture broth of t. glabrata ... | 2006 | 16972982 |
| caryophyllene-rich rhizome oil of zingiber nimmonii from south india: chemical characterization and antimicrobial activity. | volatile oil from the rhizomes of zingiber nimmonii (j. graham) dalzell was isolated, characterized by analytical gas chromatography and gas chromatography-mass spectroscopy. sixty-five constituents accounting for 97.5% of the oil were identified. z. nimmonii rhizome oil is a unique caryophyllene-rich natural source with isomeric caryophyllenes, beta-caryophyllene (42.2%) and alpha-humulene (alpha-caryophyllene, 27.7%), as its major constituents along with traces of isocaryophyllene. the rhizome ... | 2006 | 16973189 |
| pex genes in fungal genomes: common, rare or redundant. | pex genes encode proteins, termed peroxins, that are required for the biogenesis and proliferation of microbodies (peroxisomes). we have screened the available protein and dna databases to identify putative peroxin orthologs in 17 fungal species (yeast and filamentous fungi) and in humans. this analysis demonstrated that most peroxins are present in all fungi under study. only pex16p is absent in most yeast species, with the exception of yarrowia lipolytica, but this peroxin is present in all fi ... | 2006 | 16978390 |
| [emerging azole antifungals]. | azoles have maintained a key role in the treatment of invasive fungal infections which have a growing importance, especially in immunocompromised patients. because of its good activity and safety, fluconazole has becoming the treatment of choice for invasive candidiasis, except those caused by candida glabrata and c. krusei. in contrast, itraconazole which is active against aspergillus sp has limited use for the treatment of invasive mould infections due to the high variability of its bioavailib ... | 2006 | 16989123 |
| antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents. | the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ... | 2006 | 16989308 |
| influence of acrylic resin polymerization methods and saliva on the adherence of four candida species. | there is limited information on the role of polymerization methods and saliva on the adherence of pathogenic candida species, with the exception of the adherence of candida albicans to acrylic resins and the relation of this to surface roughness and surface free energy, which appear to play a major role in the initial phases of microorganism adhesion. | 2006 | 16990072 |
| candida glabrata fungemia in a diabetic patient with neurogenic bladder: successful treatment with micafungin. | a 69-year-old man was transferred to our hospital because of fever and acute renal failure. 5 weeks prior to admission, he was admitted to another hospital and treated with several antibiotics including vancomycin, but fever did not subside and renal dysfunction showed rapid progression. on admission, laboratory findings revealed pyuria, inflammatory changes, acute renal failure, and disseminated intravascular coagulation (dic). computed tomography showed left ureteral stone and hydronephrosis. ... | 2006 | 16995345 |
| boric acid for refractory candida glabrata vaginitis. | 2006 | 17000521 | |
| redistribution of carbon flux in torulopsis glabrata by altering vitamin and calcium level. | manipulation of cofactor (thiamine, biotin and ca(2+)) levels as a potential tool to redistribute carbon flux was studied in torulopsis glabrata. with sub-optimization of vitamin in fermentation medium, the carbon flux was blocked at the key node of pyruvate, and 69 g/l pyruvate was accumulated. increasing the concentrations of thiamine and biotin could selectively open the valve of carbon flux from pyruvate to pyruvate dehydrogenase complex, the pyruvate carboxylase (pc) pathway and the channel ... | 2007 | 17008113 |
| combined antifungal therapy in a murine infection by candida glabrata. | to develop proper treatments for patients who do not respond to current antifungal treatments, we tested new combinations of antifungal drugs for treating disseminated infections by candida glabrata in a murine model. | 2006 | 17012301 |
| etiologic agents of cervicovaginitis in turkish women. | to investigate the distribution of microbiologic agents causing cervicovaginitis. | 2006 | 17013472 |
| global surveillance of in vitro activity of micafungin against candida: a comparison with caspofungin by clsi-recommended methods. | micafungin is an echinocandin antifungal agent that has recently been approved for the prevention of invasive fungal infection and the treatment of esophageal candidiasis. prospective sentinel surveillance for the emergence of in vitro resistance to micafungin among invasive candida sp. isolates is indicated. we determined the in vitro activity of micafungin against 2,656 invasive (bloodstream or sterile site) unique patient isolates of candida spp. collected from 60 medical centers worldwide in ... | 2006 | 17021079 |
| candida rugosa, an emerging fungal pathogen with resistance to azoles: geographic and temporal trends from the artemis disk antifungal surveillance program. | candida rugosa is a fungus that appears to be emerging as a cause of infection in some geographic regions. we utilized the extensive database of the artemis disk antifungal surveillance program to describe the geographic and temporal trends in the isolation of c. rugosa from clinical specimens and the in vitro susceptibilities of 452 isolates to fluconazole and voriconazole. c. rugosa accounted for 0.4% of 134,715 isolates of candida, and the frequency of isolation increased from 0.03% to 0.4% o ... | 2006 | 17021085 |
| zeocin resistance as a dominant selective marker for transformation and targeted gene deletions in candida glabrata. | many of the genetic tools used to generate gene knockouts in candida glabrata exploit auxotrophic markers but this is not suitable for use with clinical strains. antibiotic resistance markers, however, allow one to target genes to be deleted without any prior genetic manipulation of clinical isolates. such antibiotic selection markers have been widely reported for the manipulation of saccharomyces cerevisiae. however, very few antibiotic resistance markers have been shown to be useful in c. glab ... | 2006 | 17022759 |
| a 5-year (2000-2004) epidemiological survey of candida and non-candida yeast species causing vulvovaginal candidiasis in graz, austria. | vulvovaginal candidasis (vvc) is a common disease. the majority of cases is caused by candida albicans, but in recent years an increase has been observed in the frequency of non-albicans candida infections, especially due to c. glabrata and c. tropicalis. the aim of the study was to assess the prevalence of non-albicans candida infections in patients suffering from vvc. therefore, the statistical data of culture-confirmed vvc ascertained at the institute of hygiene (medical university graz) have ... | 2006 | 17022763 |
| [epidemiology of candidemia: results of a one month french hospitals-based surveillance study in 2004]. | the aim of our study was to determinate the frequency of candidemia, the species encountered and their susceptibility to antifungal agents in french hospitals in 2004. | 2006 | 17029814 |
| [restropective investigation about the treatment of candidaemia in a french university hospital in 2004]. | this study aimed to retrospectively evaluate the adequate use of antifungal drugs in the treatment of the candidaemia. a collection of clinical, biological and therapeutic data was carried out for the patients who had a positive blood culture for candida species during the year 2004. the antifungal therapy was compared to the guidelines of the french conference of consensus named "prise en charge des candidoses et aspergilloses invasives de l'adulte". the degree of conformity was classified as f ... | 2006 | 17045421 |
| the bzip transcription factor cgap1p is involved in multidrug resistance and required for activation of multidrug transporter gene cgflr1 in candida glabrata. | transcriptional regulation in response to environmental challenges is crucial for survival of many organisms. in this study, we characterized structural and functional properties of cgap1, a saccharomyces cerevisiae yap1 ortholog, which encodes a transcription factor involved in various stress responses. deletion of cgap1 led to decreased resistance to hydrogen peroxide, 4-nitroquinoline-n-oxide (4-nqo), benomyl, and cadmium chloride, which could be fully recovered by reintroduction of an intact ... | 2007 | 17046176 |
| [successful treatment with caspofungin of an invasive candidosis resistant to liposomal amphotericin b in a neutropenic patient]. | 2006 | 17049216 | |
| three 1-thio-beta-d-glucopyranosides from the seeds of afrostyrax lepidophyllus mildbr. | three new 1-thioglycosides namely methylthiomethyl 1-thio-beta-d-glucopyranoside (afrostyraxthioside a), methylsulfonylmethyl 1-thio-beta-d-glucopyranoside (afrostyraxthioside b) and methylsulfonylmethylthiomethyl 1-thio-beta-d-glucopyranoside (afrostyraxthioside c) were isolated from the seeds of afrostyrax lepidophyllus mildbr. their structures were mainly elucidated by using one- and two-dimensional nmr and mass spectroscopies and also by an efficient one-step synthesis. moreover, afrostyraxt ... | 2006 | 17054924 |
| in vitro killing of nosocomial pathogens by acid and acidified nitrite. | exposure to ph 1 or 2 buffers or acidic gastric contents resulted in the killing of vancomycin-resistant enterococcus sp., klebsiella pneumoniae, staphylococcus aureus, and candida glabrata but not clostridium difficile spores. nitrite enhanced killing under acidic conditions, but significant killing of c. difficile spores required nitrite concentrations above usual physiological levels. | 2006 | 17065628 |
| caspofungin for invasive candidiasis at a tertiary care medical center. | caspofungin is emerging as first-line therapy for invasive candidiasis. data on the use of caspofungin for treatment for invasive candidiasis are limited to clinical trials and case reports. we report a single-center experience with 104 consecutive courses of caspofungin for the treatment of invasive candidiasis to evaluate a real-world performance of this drug. | 2006 | 17071169 |
| heterochromatin-mediated control of virulence gene expression. | in recent years, the sequencing and annotation of complete genomes, together with the development of genetic and proteomic techniques to study previously intractable eukaryotic microbes, has revealed interesting new themes in the control of virulence gene expression. families of variantly expressed genes are found adjacent to telomeres in the genomes of both pathogenic and non-pathogenic organisms. this subtelomeric dna is normally heterochromatic and higher-order chromatin structure has now com ... | 2006 | 17076663 |
| ethnicity and other possible risk factors for candidemia at 3 tertiary care university hospitals in hawaii. | a total of 108 cases of candidemia detected in 3 tertiary care university hospitals in hawaii between january 2001 and december 2002 were retrospectively reviewed. candida albicans, candida glabrata, and candida tropicalis accounted for 28% of the cases. mortality among filipino patients was significantly higher than that among other ethnic groups (71% vs 48%; p<.05). | 2006 | 17080389 |
| [essential genes as potential targets of antifungal agents in pathogenic yeast candida]. | an important point in the development of an antimicrobial agent is whether its target molecules are essential for growth of the microorganism. from this viewpoint, we focused attention on essential genes as potential targets of antifungal agents in the pathogenic yeast candida. here we introduce recent attempts for screening, identification, and characterization of essential genes from a haploid yeast candida glabrata, using temperature-sensitive mutants. our experimental results suggesting the ... | 2006 | 17086158 |
| in vitro effects of micafungin against candida biofilms on polystyrene and central venous catheter sections. | long-term inserted and surgically implanted catheters can be colonised by candida spp. candida biofilms in vitro are often resistant to antifungal agents. the aim of this study was to investigate the in vitro activity of micafungin (mfg) against six candida spp. biofilms on polystyrene (ps) and central venous catheter (cvc) sections. safranin staining and differential interference contrast microscopy were used to demonstrate biofilm production. mfg activity was determined by the reduction in met ... | 2006 | 17101265 |
| a fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis. | to date, most fungal phylogenies have been derived from single gene comparisons, or from concatenated alignments of a small number of genes. the increase in fungal genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. as a tool for future comparative, phylogenomic and phylogenetic studies, we used both supertrees and concatenated alignments to infer relationships between 42 species of fungi for which complete genome sequences are available. | 2006 | 17121679 |
| [changing epidemiological aspects of candidemia and their clinical and therapeutic implications]. | candida species are a major cause of healthcare-related bloodstream and invasive infections. studies assessing nosocomial bloodstream infections during the two last decades ranked candida species as the fourth most common nosocomial bloodstream pathogen. the incidence of candida species has risen steadily during this period due to the increase in the number and type of patients at risk for these yeasts. infections caused by candida are especially frequent and serious in onco-hematological patien ... | 2006 | 17125667 |
| synthesis and antimicrobial activity of 1-(4-aryl-2-thiazolyl)-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives. | several 1-(4-aryl-2-thiazolyl)-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives were synthesized by reacting substituted 3-(2-thienyl)-5-aryl-1-thiocarbamoyl-2-pyrazolines with phenacyl bromides in ethanol. the structures of the synthesized compounds were confirmed by (1)h nmr, (13)c nmr, and eims spectral data. their antimicrobial activities against escherichia coli (nrrl b-3704), staphylococcus aureus (nrll b-767), salmonella typhimurium (nrrl b-4420), bacillus cereus (nrrl b-3711), streptococcus ... | 2007 | 17125888 |
| [disk diffusion method for fluconazole susceptibility testing of candida spp. isolates]. | in order to standardize and evaluate a disk diffusion method with visual reading to detect in vitro fluconazole susceptibility of yeast, 1193 clinical isolates were tested. these included 584 candida albicans, 196 candida parapsilosis, 200 candida tropicalis, 113 candida glabrata, 50 candida krusei and 50 candida spp. and other opportunistic yeasts. the disks were manufactured in the inei-anlis "dr. carlos g. malbrán. the disk diffusion method results were compared to mic results obtained by the ... | 2006 | 17152217 |
| enhancement of pyruvate production by osmotic-tolerant mutant of torulopsis glabrata. | pyruvate production by torulopsis glabrata was used as a model to study the mechanism of product inhibition and the strategy for enhancing pyruvate production. it was found that the concentration of cell growth and pyruvate deceased with the increase of nacl and sorbitol concentrations. to enhance the osmotic stress resistance of the strain, an nacl-tolerant mutant rs23 was screened and selected through a ph-controlled continuous culture with 70 g/l nacl as the selective criterion. compared with ... | 2007 | 17154310 |
| reduced susceptibility to polyenes associated with a missense mutation in the erg6 gene in a clinical isolate of candida glabrata with pseudohyphal growth. | little information is available about the molecular mechanisms responsible for polyene resistance in pathogenic yeasts. a clinical isolate of candida glabrata with a poor susceptibility to polyenes, as determined by disk diffusion method and confirmed by determination of mic, was recovered from a patient treated with amphotericin b. quantitative analysis of sterols revealed a lack of ergosterol and an accumulation of late sterol intermediates, suggesting a defect in the final steps of the ergost ... | 2007 | 17158937 |
| nad+ metabolism in health and disease. | nicotinamide adenine dinucleotide (nad(+)) is both a coenzyme for hydride-transfer enzymes and a substrate for nad(+)-consuming enzymes, which include adp-ribose transferases, poly(adp-ribose) polymerases, cadp-ribose synthases and sirtuins. recent results establish protective roles for nad(+) that might be applicable therapeutically to prevent neurodegenerative conditions and to fight candida glabrata infection. in addition, the contribution that nad(+) metabolism makes to lifespan extension in ... | 2007 | 17161604 |
| capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate. | the nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. this dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. the values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pi markers and were found comparable with pi of the native compounds. the m ... | 2006 | 17165837 |
| amphotericin b in the therapy of candida glabrata endophthalmitis after penetrating keratoplasty. | candida glabrata is a rare cause of endophthalmitis after penetrating keratoplasty. adequate therapy is still under discussion. with respect to severe complications and side effects of antifungal therapy, a substantial knowledge of sensitivity and resistance of the organism is necessary. | 2006 | 17172909 |
| antifungal resistance in candida spp. isolated in italy between 2002 and 2005 from children and adults. | the activity of amphotericin b, fluconazole, flucytosine, itraconazole and voriconazole was tested in vitro against 618 clinical candida spp. isolates, using the broth microdilution or the disk diffusion method (voriconazole). amphotericin b and voriconazole were the most potent antifungal agents assayed (100% of susceptible strains). resistance to fluconazole and itraconazole was detected in three (0.7%) and 11 (2.7%) isolates of candida albicans and in four (3.7%) isolates of candida glabrata. ... | 2007 | 17175140 |
| [isolation rate and antifungal drug susceptibility of yeast like fungi isolated from blood or vascular catheter]. | increased resistance of strains to antifungal drugs has gained increasing attention. we studied the status of fungal isolation from blood and vascular catheters at juntendo university hospital from 1994 to 2002. the major fungi isolated were candida albicans, candida parapsilosis, candida glabrata and candida trophicalis, or 86% yeast-like fungi. isolation of these fungi from vascular catheters is increasing. the effectiveness of 6 anti-fungal agents against 116 yeast-like fungi was measured by ... | 2006 | 17176852 |
| development of candidemia on caspofungin therapy: a case report. | caspofungin, an echinocandin, is approved for use in invasive candidiasis. few cases of break-through candidal infections during caspofungin therapy have been reported and none have involved candida parapsilosis. here, we report a patient who developed multiple post-operative complications after pancreaticoduodenectomy for a pancreatic mass, including fungemia due to c. parapsilosis, while on caspofungin for treatment of candida glabrata peritonitis. the fungemia resolved after a central venous ... | 2006 | 17180591 |
| synthesis and evaluation of novel 1-(1h-1,2,4-triazol-1-yl)-2-(2,4-difluorophenyl)-3-[(4-substitutedphenyl)-piperazin-1-yl]-propan-2-ols as antifungal agents. | a series of 1-(1h-1,2,4-triazol-1-yl)-2-(2,4-difluorophenyl)-3-[(4-substitutedphenyl)-piperazin-1-yl]-propan-2-ols have been designed and synthesized on the basis of the structure-activity relationships and antimycotic mechanism of azole antifungal agents. their structures were confirmed by elemental analysis, ir, ms, (1)h nmr and (13)c nmr. results of preliminary antifungal tests against six human pathogenic fungi (candida albicans, candida parapsilosis, cryptococcus neoformans, candida tropica ... | 2007 | 17184885 |
| norlignans from sequoia sempervirens. | six new norlignans, named sequosempervirins b-g (1-6), together with three known norlignans, agatharesinol (7), agatharesinol acetonide (8), and sugiresinol (9), were isolated from the branches and leaves of sequoia sempervirens. their structures were determined mainly by high-resolution mass spectroscopy (hr-ms), and various 1d- and 2d-nmr methods, as well as, in the case of 1, by means of x-ray diffraction. compound 8 showed anticancer activity towards the a549 non-small-cell lung-cancer cell ... | 2005 | 17191998 |
| [identification of candida species causing vaginitis in mexican patients]. | the increasing frequency of candidiasis due candida spp. other than c. albicans suggests that these organisms may also have emerged as a cause of vaginitis. | 2006 | 17194389 |
| consistent patterns of rate asymmetry and gene loss indicate widespread neofunctionalization of yeast genes after whole-genome duplication. | we investigated patterns of rate asymmetry in sequence evolution among the gene pairs (ohnologs) formed by whole-genome duplication (wgd) in yeast species. by comparing three species (saccharomyces cerevisiae, candida glabrata, and s. castellii) that underwent wgd to a nonduplicated outgroup (kluyveromyces lactis), and by using a synteny framework to establish orthology and paralogy relationships at each duplicated locus, we show that 56% of ohnolog pairs show significantly asymmetric protein se ... | 2007 | 17194778 |
| once-weekly micafungin therapy is as effective as daily therapy for disseminated candidiasis in mice with persistent neutropenia. | the effect of micafungin dose scheduling on the treatment of candidemia is unknown. neutropenic mice with disseminated candida glabrata infection were treated with single intraperitoneal micafungin doses of 0 to 100 mg/kg of body weight and sacrificed 7 days later. the maximal decline in kidney fungal burden was 5.8 log(10) cfu/g. a 1-week pharmacokinetic-pharmacodynamic study revealed a micafungin serum half-life of 6.13 h. in mice treated with > or =50 mg/kg, there was maximal fungal decline w ... | 2007 | 17194830 |
| comparative evaluation of etest and sensititre yeastone panels against the clinical and laboratory standards institute m27-a2 reference broth microdilution method for testing candida susceptibility to seven antifungal agents. | to assess their utility for antifungal susceptibility testing in our clinical laboratory, the etest and sensititre methods were compared with the clinical and laboratory standards institute (clsi) m27-a2 reference broth microdilution method. fluconazole (fl), itraconazole (i), voriconazole (v), posaconazole (p), flucytosine (fc), caspofungin (c), and amphotericin b (a) were tested with 212 candida isolates. reference mics were determined after 48 h of incubation, and etest and sensititre mics we ... | 2007 | 17202279 |
| chemosensitisation of drug-resistant and drug-sensitive yeast cells to antifungals. | multidrug resistance in yeast results from overexpression of genes encoding drug efflux transporters owing to gain-of-function mutations in transcription factors regulating their expression. we have screened a library of synthetic compounds for modulators of drug resistance using the multidrug-resistant saccharomyces cerevisiae pdr3-9 mutant strain. one of the compounds, 7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine (ctbt), displayed weak antifungal activity and strongly inhibited the growth of y ... | 2007 | 17204400 |
| multicenter comparison of the vitek 2 yeast susceptibility test with the clsi broth microdilution reference method for testing fluconazole against candida spp. | a fully automated commercial antifungal susceptibility test system (vitek 2 yeast susceptibility test; biomerieux, inc., hazelwood, mo.) was compared in three different laboratories with clinical and laboratory standards institute (clsi) reference broth microdilution (bmd) method by testing two quality control strains and a total of 426 isolates of candida spp. (103 to 135 clinical isolates in each laboratory plus 80 challenge isolates in one laboratory) against fluconazole. reference bmd mic en ... | 2007 | 17215344 |
| detection of herpes simplex virus and candida infection with special emphasis on their possible role in human squamous cell carcinoma and its variants. | sixty seven cases of human oral s.c.c. with its intra oral variances were investigated microbiologically, histopathologically, histochemically and immunologically. yeasts were isolated from 85% of examined cases. "candifast test" showed that candida albicans was the commonest form of the detected fungi, followed by c. parapsilosis and c. tropicalis, while the least in frequency was the torulopsis glabrata. yeasts were more detected in females, elders and tobacco smokers. two cases of well differ ... | 1996 | 17217014 |
| epidemiology of invasive candidiasis: a persistent public health problem. | invasive candidiasis (ic) is a leading cause of mycosis-associated mortality in the united states. we examined data from the national center for health statistics and reviewed recent literature in order to update the epidemiology of ic. ic-associated mortality has remained stable, at approximately 0.4 deaths per 100,000 population, since 1997, while mortality associated with invasive aspergillosis has continued to decline. candida albicans remains the predominant cause of ic, accounting for over ... | 2007 | 17223626 |
| sensitivity of peripheral blood smear review for the diagnosis of candida fungemia. | case reports have described detection of candidemia by examination of peripheral blood smears. it is unclear whether this method has wider applicability for early detection of fungemia. | 2007 | 17227130 |
| species distribution and antifungal susceptibility of candida bloodstream isolates in kuwait: a 10-year study. | bloodstream infections due to candida species are important complications in severely ill hospitalized patients. this study presents data on species distribution and antifungal susceptibility profiles of candida bloodstream isolates obtained from kuwait during a 10-year period. all the bloodstream isolates were identified to species level by the germ tube test and carbohydrate assimilation profile using the vitek 2 yeast identification system. using e-test strips for amphotericin b, fluconazole, ... | 2007 | 17244809 |
| development of novel real-time pcr assays for detection and differentiation of eleven medically important aspergillus and candida species in clinical specimens. | in the present study, novel real-time pcr assays targeting the fungal its2 region were developed for the detection and differentiation of medically important aspergillus species (aspergillus fumigatus, aspergillus flavus, aspergillus nidulans, aspergillus niger, and aspergillus terreus) and candida species (candida albicans, candida dubliniensis, candida glabrata, candida krusei, candida parapsilosis, and candida tropicalis) using a lightcycler instrument. the combination of a group-specific and ... | 2007 | 17251398 |
| simvastatin reduces ergosterol levels, inhibits growth and causes loss of mtdna in candida glabrata. | statins are widely used for lowering cholesterol levels through their action on 3-hydroxy-3-methylglutaryl-coa (hmg-coa) reductase. yeasts use hmg-coa reductase for the same enzymatic step as humans, but in yeasts the main end-product of the pathway is ergosterol rather than cholesterol. we considered that insights into the effects of statins in humans could be gained by examination of the effects of simvastatin on the petite-positive yeast candida glabrata. simvastatin was found to inhibit grow ... | 2007 | 17257373 |
| prevalence of candida glabrata and its response to boric acid vaginal suppositories in comparison with oral fluconazole in patients with diabetes and vulvovaginal candidiasis. | a large proportion of vulvovaginal candidiasis (vvc) in diabetes is due to non-albicans candida species such as c. glabrata and c. tropicalis. observational studies indicate that diabetic patients with c. glabrata vvc respond poorly to azole drugs. we evaluated the response to oral fluconazole and boric acid vaginal suppositories in diabetic patients with vvc. | 2007 | 17259500 |
| application of response surface methodology in medium optimization for pyruvic acid production of torulopsis glabrata tp19 in batch fermentation. | response surface methodology (rsm) was used to optimize the fermentation medium for enhancing pyruvic acid production by torulopsis glabrata tp19. in the first step of optimization, with plackett-burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. in the second step, a 2(3) full factorial central composite design and rsm were applied to determine the optimal concentration of each significant variable. ... | 2007 | 17266184 |
| five-hour diagnosis of dermatophyte nail infections with specific detection of trichophyton rubrum. | a rapid two-step dna extraction method and a multiplex pcr for the detection of dermatophytes in general and trichophyton rubrum specifically were developed and evaluated with dna extracted from pure cultures and from clinically diseased nails. dna from the following dermatophytes was used: epidermophyton floccosum, microsporum audouinii, microsporum canis, microsporum gypseum, microsporum nanum, trichophyton mentagrophytes, trichophyton rubrum, trichophyton schoenleinii, trichophyton soudanense ... | 2007 | 17267633 |
| bacterial isolates from fatal cases of bloodstream infections at a university hospital in central, saudi arabia. | to describe the microbiology, underlying medical conditions and risk factors contributing to bloodstream infection (bsi) and mortality at a university hospital in riyadh, kingdom of saudi arabia. | 2007 | 17268702 |
| caspofungin in the treatment of symptomatic candiduria. | because the urine concentrations achieved by echinocandin antifungal agents are low, drugs from this class are excluded from consideration when candiduria treatment is selected. | 2007 | 17278048 |
| inadequacy of fluconazole dosing in patients with candidemia based on infectious diseases society of america (idsa) guidelines. | based on infectious diseases society of america (idsa) guidelines, inappropriate fluconazole therapy in patients with candidemia is defined as an empiric dose <6 mg/kg/d, <12 mg/kg/d after candida glabrata identification, or continued fluconazole use after identification of candida krusei. however, the extent to which inappropriate antifungal therapy is due to improper dosing or drug selection has not been well investigated. the objectives of this study were to assess the incidence of inappropri ... | 2007 | 17286303 |
| in vitro susceptibility of candida species to five antifungal agents in a german university hospital assessed by the reference broth microdilution method and etest. | the aim of this study is to evaluate the susceptibilities of candida spp. to the common antifungal agents in a german university hospital. since quick results of in vitro testing are desirable, etest and the clsi broth microdilution (bmd) method (reference method) were compared, focusing on the validity of early readings. | 2007 | 17293369 |
| cytotoxic and cytokine-inducing properties of candida glabrata in single and mixed oral infection models. | oral candidiasis is a common opportunistic infection, with candida albicans being the most prevalent etiologic agent and candida glabrata emerging as an important pathogen. c. glabrata is frequently co-isolated with c. albicans from oral lesions. although c. albicans has been shown to trigger significant cytokine responses and cell damage, c. glabrata has not been systematically studied yet. the purpose of this study was to characterize the ability of c. glabrata to induce proinflammatory cytoki ... | 2007 | 17306958 |
| in vivo efficacy of anidulafungin and caspofungin against candida glabrata and association with in vitro potency in the presence of sera. | in vitro studies have demonstrated that anidulafungin has greater potency than caspofungin against candida glabrata. however, data from in vivo studies demonstrating that it has superior efficacy are lacking. the objective of this study was to compare the activities of anidulafungin and caspofungin against c. glabrata in a murine model of disseminated candidiasis. two clinical c. glabrata isolates were used, including one with reduced caspofungin susceptibility. mics were determined by broth mic ... | 2007 | 17307976 |
| fluconazole susceptibility of candida spp. isolates collected over nine years in a teaching hospital of ancona, italy. | the in vitro activity of fluconazole was investigated against 476 yeast isolates collected during a 9-year period (1997-2005) from patients hospitalised in a teaching hospital of ancona. they included 373 isolates of candida albicans, 53 of candida glabrata and 50 of candida parapsilosis. minimum inhibitory concentrations (mics) determined in accordance with the clinical laboratory standards institute methodology showed that 96% of the isolates were susceptible (mic < or =8.0 microg/ml). the unc ... | 2007 | 17309852 |
| do clinical features allow for accurate prediction of fungal pathogenesis in bloodstream infections? potential implications of the increasing prevalence of non-albicans candidemia. | to describe the evolving epidemiology of fungal bloodstream infections in critically ill and noncritically ill patients and to identify predictors of infection with non-albicans yeast species. | 2007 | 17312565 |
| candida glabrata: an emerging oral opportunistic pathogen. | following the widespread use of immunosuppressive therapy and broad-spectrum antimycotic prophylaxis, c. glabrata has emerged as an important opportunistic pathogen in the oral mucosa. in the past, studies on the virulence factors and host-pathogen interactions of this organism were scarce, but continued to rise in recent years. denture-wearing, immunosuppression, antibiotic therapy, and aging are risk factors for oral colonization or infection with c. glabrata. compared with c. albicans, c. gla ... | 2007 | 17314251 |
| in vitro biofilm formation of candida albicans and non-albicans candida species under dynamic and anaerobic conditions. | an understanding of biofilm behavior of candida species under different environmental conditions is key to the development of effective preventive measures for candidal infections. hence in this study we assessed the impact of the environmental milieu on candida biofilm formation using polystyrene, flat-bottomed 96-well microtiter plates. a total of 20, comprising 10 clinical isolates each of candida albicans and, non-albicans species of candida were compared for their biofilm forming ability bo ... | 2007 | 17321487 |
| infectious gastritis caused by a mixed fungal and bacterial infection. | 2006 | 17366429 | |
| prospective evaluation of the new chromogenic medium candiselect 4 for differentiation and presumptive identification of the major pathogenic candida species. | the rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. candiselect 4 (cs4; bio-rad) is a new chromogenic medium for the isolation of fungi, the direct identification of candida albicans and the presumptive identification of the major pathogenic candida species. the performance of cs4 was compared with that of another chromogenic medium, chromagar candida (ca; becton dickinson). for primary cultures, 502 of ... | 2007 | 17374890 |
| effects of dosing regimen on accumulation, retention and prophylactic efficacy of liposomal amphotericin b. | we hypothesized that effective prophylactic treatment of fungal infections would require adequate drug penetration and retention at potential infection sites. using a mouse model, we examined liposomal amphotericin b (l-amb) biodistribution, cell localization and retention in kidneys, lungs, liver and spleen to evaluate effective dosing regimens for prophylaxis of candida glabrata and candida albicans infections. | 2007 | 17400589 |
| intracardiac giant cells after left ventricular assist device placement. | multinucleated giant cells are seen in the heart in several distinct pathologic conditions, including giant cell myocarditis, sarcoidosis and infectious etiologies. we describe the first report of solitary multinucleated giant cells scattered throughout the heart after placement of a left ventricular assist device (lvad) and transient candida glabrata infection. the pathologic findings were noted in the explanted heart of a patient undergoing orthotopic cardiac transplantation 7 months after lva ... | 2007 | 17403488 |
| to test or not to test: a cost minimization analysis of susceptibility testing for patients with documented candida glabrata fungemias. | this cost minimization analysis investigated the financial impact of the treatment of fungemias due to candida glabrata from a hospital perspective using three competing alternatives: (i) performing in-house susceptibility testing on all c. glabrata isolates and changing patients to less expensive fluconazole therapy for isolates that test susceptible; (ii) susceptibility testing at outside laboratories with delayed deescalation to fluconazole if isolates test susceptible; and (iii) no routine s ... | 2007 | 17409208 |
| a yeast related ethanol intoxication syndrome in experimental calves: prevention with nystatin. | in a calf nutrition-metabolism study in which a high glucose (60% glucose and 3% lard) versus a high fat (23% glucose and 30% lard) milk replacer were compared, a syndrome characterized by anorexia, an unstable gait, depression, a distended abdomen and an odor of alcohol on the breath was observed in some calves. these signs were associated with intoxicating levels of plasma ethanol and the predominance of the yeast species torulopsis glabrata in gastrointestinal contents of afflicted calves. ny ... | 1984 | 17422416 |
| why do some yeast species require niacin for growth? different modes of nad synthesis. | nad holds a key position in metabolism and cellular regulatory events as a major redox carrier and a signalling molecule. nad biosynthesis pathways have been reconstructed and compared in seven yeast species with completely sequenced genomes, including saccharomyces cerevisiae, kluyveromyces lactis, candida glabrata, debaryomyces hansenii, candida albicans, yarrowia lipolytica and schizosaccharomyces pombe. both amino acid and nucleotide sequence similarity analysis in silico indicated that de n ... | 2007 | 17425674 |