Publications

enzyme activities and arylsulfatase protein content of dust and the soil source: biochemical fingerprints?	little is known about the potential of enzyme activities, which are sensitive to soil properties and management, for the characterization of dust properties. enzyme activities may be among the dust properties key to identifying the soil source of dust. we generated dust (27 and 7 microm) under controlled laboratory conditions from agricultural soils (0-5 cm) with history of continuous cotton (gossypium hirsutum l.) or cotton rotated with peanut (arachis hypogaea l.), sorghum [sorghum bicolor (l. ...	2006	15356225
aquaporin homologues in plants and mammals transport ammonia.	using functional complementation and a yeast mutant deficient in ammonium (nh4+) transport (deltamep1-3), three wheat (triticum aestivum) tip2 aquaporin homologues were isolated that restored the ability of the mutant to grow when 2 mm nh4+ was supplied as the sole nitrogen source. when expressed in xenopus oocytes, tatip2;1 increased the uptake of nh4+ analogues methylammonium and formamide. furthermore, expression of tatip2;1 increased acidification of the oocyte-bathing medium containing nh4+ ...	2004	15358535
1h magnetization transfer in hydrated gluten and flour: effects of wheat aging.	the interaction of water with flour or gluten in hydrated samples was investigated by proton magnetization transfer measurements. flour and gluten from both durum and bread wheat seeds, either unaged or artificially aged over different periods of time, were investigated. measurements were performed at several radio frequency power levels and frequency offsets, and the data were quantitatively modeled by two interacting pools, a liquid (water) and a solid (macromolecules) one. a super-lorentzian ...	2017	15360294
construction of a subgenomic bac library specific for chromosomes 1d, 4d and 6d of hexaploid wheat.	the analysis of the hexaploid wheat genome (triticum aestivum l., 2 n=6 x=42) is hampered by its large size (16,974 mb/1c) and presence of three homoeologous genomes (a, b and d). one of the possible strategies is a targeted approach based on subgenomic libraries of large dna inserts. in this work, we purified by flow cytometry a total of 10(7) of three wheat d-genome chromosomes: 1d, 4d and 6d. chromosomal dna was partially digested with hindiii and used to prepare a specific bacterial artifici ...	2004	15365624
wheat (triticum vulgare) chloroplast nuclease chsi exhibits 5' flap structure-specific endonuclease activity.	the structure-specific chsi nuclease from wheat (triticum vulgare) chloroplast stroma has been previously purified and characterized in our laboratory. it is a single-strand-specific dna and rna endonuclease. although the enzyme has been initially characterized and used as a structural probe, its biological function is still unknown. localization of the chsi enzyme inside chloroplasts, possessing their own dna that is generally highly exposed to uv light and often affected by numerous redox reac ...	2004	15366938
[comparative characteristics of reproduction of the wheat striped mosaic virus in winter and spring triticum aestivum l. in natural agrocoenosis and during clinostatting].	microgravity (a transformed environment) was produced with the use of a multi-purpose clinostat. object of the investigation was wheat striped mosaic virus (wsmv) affecting a great variety of wheat species in natural agrocoenosis, and super-dwarf cultivar apogee in the transformed environment. enzyme immunodetection (das-elisa) as well as electron microscopy were employed for virus identification. viral reproduction was found high (titre 1/2560) in winter and spring wheat species in agrocoenosis ...	2006	15372798
interactions among factors regulating phenological development and acclimation rate determine low-temperature tolerance in wheat.	exposure to low temperatures (lt) produces innumerable changes in morphological, biochemical and physiological characteristics of plants, with the result that it has been difficult to separate cause and effect adjustments to lt. phenotypic studies have shown that the lt-induced protective mechanisms in cereals are developmentally regulated and involve an acclimation process that can be stopped, reversed and restarted. the present study was initiated to separate the developmental factors determin ...	0	15374834
characterization of the common wheat (triticum aestivum l.) mutation line producing three pistils in a floret.	in a normal wheat (triticum ssp.l.) spike, one floret carries only one pistil that will further develop into one grain after fertilization. the cultivated common wheat (t. aestivum l.) mutation line three pistils (tp) carried three pistils in a floret. although one or two of the pistils died out before seed set in some florets, there were exist many florets that set three seeds. normally, it was observed that there were one to three seeds in different florets of the same spike. therefore, this m ...	2004	15383067
molecular cloning and comparative analysis of a y-type inactive hmw glutenin subunit gene from cultivated emmer wheat (triticum dicoccum l.).	cultivated emmer (triticum dicoccum, 2n = 4x = 28, aabb) is closely related to bread wheat and possesses extensive allelic variations in high molecular weight glutenin subunit (hmw-gs) composition. these alleles may be an important genetic resource for wheat quality improvement. to isolate and clone hmw-gs genes from cultivated emmer, two pairs of allele-specific (as) pcr primers were designed to amplify the coding sequence of y-type hmw-gs genes and their upstream sequences, respectively. the r ...	2004	15383071
auxin induces an increase of ca2+ concentration in the cytosol of wheat leaf protoplasts.	auxin addition to protoplasts isolated from leaves of 6-day-old wheat seedlings (triticum aestivum l. cv. kadett) induced a rapid increase in the cytosolic calcium concentration [ca2+]cyt. the shifts in [ca2+]cyt were detected by use of fluorescence microscopy in single protoplasts loaded with the calcium binding tetra[acetoxymethyl]ester of the fluorescent dye, fura 2. addition of the synthetic auxin naphthyl acetic acid, 1-naa, induced an increase in [ca2+]cyt within 5-10s, while the physiolog ...	2004	15384405
determination and evaluation of the sequence and textural effects of the puroindoline a and puroindoline b genes in a population of synthetic hexaploid wheat.	aegilops tauschii (2 n=2 x=14, dd) is a rich source of genetic variability for hexaploid wheat ( triticum aestivum, 2 n=6 x=42, aabbdd) improvement. this variability can be accessed through utilizing synthetic hexaploid wheat lines, which contain genomes from ae. tauschii and t. turgidum (2 n=4 x=28, aabb). numerous desirable characteristics can and have been introgressed into common hexaploid wheat with this germplasm. in this work, the genetic variability in the two puroindoline genes (a and b ...	2004	15448897
durum wheat as a candidate for the unknown female progenitor of bread wheat: an empirical study with a highly fertile f1 hybrid with aegilops tauschii coss.	hexaploid bread wheat was derived from a hybrid cross between a cultivated form of tetraploid triticum wheat (female progenitor) and a wild diploid species, aegilops tauschii coss. (male progenitor). this cross produced a fertile triploid f1 hybrid that set hexaploid seeds. the identity of the female progenitor is unknown, but various cultivated tetraploid triticum wheats exist today. genetic and archaeological evidence suggests that durum wheat ( t. turgidum ssp. durum) may be the female progen ...	2004	15448900
[effect of the 5r(5a) alien chromosome substitution on the growth habit and winter hardiness of wheat].	the growth habit, ear emergence time, and frost tolerance of wheat/rye substitution lines have been studied in cultivars rang and mironovskaya krupnozernaya whose chromosome 5a is substituted with chromosome 5r of onkhoyskaya rye. hybrid analysis has demonstrated that the spring habit of the recipient cultivars rang and mironovskaya krupnozernaya is controlled by dominant gene vrn-a1 located in chromosome 5a. onokhoyskaya rye has a dominant gene for the spring habit (sp1) located in chromosome 5 ...	2004	15458211
compositions and sorptive properties of crop residue-derived chars.	chars originating from the burning or pyrolysis of vegetation may significantly sorb neutral organic contaminants (nocs). to evaluate the relationship between the char composition and noc sorption, a series of char samples were generated by pyrolyzing a wheat residue (triticum aestivum l.) for 6 h at temperatures between 300 degrees c and 700 degrees c and analyzed for their elemental compositions, surface areas, and surface functional groups. the samples were then studied for their abilities to ...	2004	15461175
engineering high-level aluminum tolerance in barley with the almt1 gene.	acidity is a serious limitation to plant production on many of the world's agricultural soils. toxic aluminium (al) cations solubilized by the acidity rapidly inhibit root growth and limit subsequent uptake of water and nutrients. recent work has shown that the almt1 gene of wheat (triticum aestivum) encodes a malate transporter that is associated with malate efflux and al tolerance. we generated transgenic barley (hordeum vulgare) plants expressing almt1 and assessed their ability to exude mala ...	2004	15471989
transformation of fulvic substances in the rhizosphere during phytoremediation of used motor oil.	changes in the chemical composition of fulvic acids in used oil-contaminated soils treated with different plant species and fertilization (fertilized, f; and nonfertilized, nf) were analyzed by fourier-transformed infrared spectroscopy (ftir). soil contaminated with 1.5% (w/w) used motor oil was seeded with sunflower (helianthus annuus)/indian mustard (brassica juncea); soybean (glycine max)/green bean (phaseolus vulgaris); mixed grasses/maize (zea mays); mixed clover (red clover, trifolium prat ...	2004	15478929
degradation studies on benzoxazinoids. soil degradation dynamics of 2,4-dihydroxy-7-methoxy-(2h)-1,4-benzoxazin-3(4h)-one (dimboa) and its degradation products, phytotoxic allelochemicals from gramineae.	benzoxazinoids have been described as important allelochemicals from gramineae as well as acanthaceae, rannunculaceae, and scrophulariaceae plants. several bioactivities have been described and evaluated for these compounds, including fungistatic, antifeedant, and phytotoxic. in ongoing studies about allelochemicals as natural herbicide models, the description of soil dynamics in phytotoxic agents has high importance, because the possible biotransformations developed by soil microorganisms could ...	2004	15478999
an efficient method for flow cytometric analysis of pollen and detection of 2n nuclei in brassica napus pollen.	a simple and reliable method was developed for isolating pollen nuclei from brassica napus and triticum aestivum for dna analysis using flow cytometry. the nuclei were released from pollen by ultrasonic treatment. the isolated nuclei following filtration through nylon mesh and a purification procedure were suitable for flow cytometric analysis as well as for isolating genomic dna. ultrasonic treatment time was optimized for b. napus pollen at different developmental stages. the method is effecti ...	2004	15480680
[a preliminary study on gene expression profile induced by water stress in wheat (triticum aestivum l.) seedling].	in the present research, suppression subtractive hybridization (ssh) and high density membrane techniques were employed to analysis genes induced by water stress in wheat seedling at 2-leaf stage. the purpose was to comprehensively understand the genetic bases of drought resistance and to find the key genes related to drought resistance in wheat. a total of 181 positive clones were obtained by screening the ssh library including 1 530 individual recombinant clones. the result of the sequence hom ...	2004	15481541
a high-density microsatellite consensus map for bread wheat (triticum aestivum l.).	a microsatellite consensus map was constructed by joining four independent genetic maps of bread wheat. three of the maps were f(1)-derived, doubled-haploid line populations and the fourth population was 'synthetic' x 'opata', an f(6)-derived, recombinant-inbred line population. microsatellite markers from different research groups including the wheat microsatellite consortium, gwm, gdm, cfa, cfd, and barc were used in the mapping. a sufficient number of common loci between genetic maps, ranging ...	2004	15490101
quantitative analyses of relationships between ecotoxicological effects and combined pollution.	the responses of wheat triticum aestivum, rice oryza sativa, earthworms eisenia foetida, and prawns penaeus japonicus to combined acetochlor-cu, cd-zn were studied in hydroponic and soil-culturing systems using the methods of ecotoxicology. in particular, systematically quantitative analyses were documented by field experiments. results showed that ecotoxicological effects under the combined pollution were not only related to chemical properties of pollutants but also dependent on the concentrat ...	2004	15493474
nitrogen fixation in wheat provided by klebsiella pneumoniae 342.	in this report, all of the criteria necessary for the demonstration of nitrogen fixation in wheat (triticum aestivum l.), the world's most important crop, are shown upon inoculation with a nitrogen-fixing bacterium, klebsiella pneumoniae 342 (kp342). kp342 relieved nitrogen (n) deficiency symptoms and increased total n and n concentration in the plant. nitrogen fixation was confirmed by 15n isotope dilution in the plant tissue and in a plant product, chlorophyll. all of these observations were i ...	2004	15497400
development and mapping of est-derived simple sequence repeat markers for hexaploid wheat.	expressed sequence tags (ests) are a valuable source of molecular markers. to enhance the resolution of an existing linkage map and to identify putative functional polymorphic gene loci in hexaploid wheat (triticum aestivum l.), over 260,000 ests from 5 different grass species were analyzed and 5418 ssr-containing sequences were identified. using sequence similarity analysis, 156 cross-species superclusters and 138 singletons were used to develop primer pairs, which were then tested on the genom ...	2004	15499395
simultaneous painting of three genomes in hexaploid wheat by bac-fish.	fluorescence in situ hybridization (fish) is widely used in the physical mapping of genes and chromosome landmarks in plants and animals. bacterial artificial chromosomes (bacs) contain large inserts, making them amenable for fish mapping. in our bac-fish experiments, we selected 56 restriction fragment length polymorphism (rflp)-locus-specific bac clones from the libraries of triticum monococcum and aegilops tauschii, which are the a- and d-genome donors of wheat (triticum aestivum, 2n = 6x = 4 ...	2004	15499412
the pyridoxal kinase gene tapdxk from wheat complements vitamin b6 synthesis-defective escherichia coli.	pyridoxal kinase (ec 2.7.1.35) is a key enzyme in the conversion of vitamin b6 to pyridoxal 5'-phosphate (plp). plp is the crucial cofactor required by numerous enzymes involved in amino acids metabolism. recently, studies with arabidopsis salt overly sensitive 4 mutants demonstrated that pyridoxal kinase is a novel salt tolerance determinant important for the regulation of na+ and k+ homeostasis in plants. we describe here the tapdxk gene which encodes a pyridoxal kinase, cloned from triticum a ...	2004	15499907
sequence composition, organization, and evolution of the core triticeae genome.	we investigated the composition and the basis of genome expansion in the core triticeae genome using aegilops tauschii, the d-genome donor of bread wheat. we sequenced an unfiltered genomic shotgun (trs) and a methylation-filtration (tmf) library of a. tauschii, and analyzed wheat expressed sequence tags (ests) to estimate the expression of genes and transposable elements (tes). the sampled d-genome sequences consisted of 91.6% repetitive elements, 2.5% known genes, and 5.9% low-copy sequences o ...	2004	15500466
effect of salinity on tissue architecture in expanding wheat leaves.	salinity greatly reduces the leaf cross-sectional area of wheat (triticum aestivum l.) during its development, which may lead to variation in the architectural properties of growing leaves that would result in a change in leaf physiological functions. our objective was to characterize the effect of salinity on the spatial distribution of the cross-sectional area and the anatomy of large and small veins of a growing wheat leaf. spring wheat was grown in a growth chamber in soils with or without 1 ...	2005	15503127
chemical alteration of the rhizosphere of the mycorrhizal-colonized wheat root.	plexiglass pot growth chamber experiments were conducted to evaluate the chemical alterations in the rhizosphere of mycorrhizal wheat roots after inoculation with glomus intraradices [arbuscular mycorrhizal fungus (amf)]. exchange resins were used as sinks for nutrients to determine whether the inoculated plant can increase the solubility and the uptake of p and micronutrients. treatments included: (1) soil (bulk soil); (2) amf inoculation no p addition (i-p); (3) no inoculation with no p additi ...	2005	15503187
responses of female orange wheat blossom midge, sitodiplosis mosellana, to wheat panicle volatiles.	air entrainment samples of volatiles from panicles of intact wheat, triticum aestivum, cultivar 'lynx' were collected at the ear emergence/early anthesis growth stage. in an olfactometer bioassay, both freshly cut panicles and an air entrainment sample were found to attract female orange wheat blossom midge adults, sitodiplosis mosellana. coupled gas chromatography-electroantennography (gc-eag) analyses of panicle volatiles located six electrophysiologically active components. these were identif ...	2004	15503522
retention and phytoavailability of radioniobium in soils.	radioniobium is present in long-lived nuclear waste as a result of the activation of zirconium pellets associated with the nuclear fuel. the behaviour of niobium (nb) in the environment and especially its fate in the soil-plant system has not been thoroughly investigated so far. in safety assessment of french long-lived nuclear waste disposal, data concerning the mobility and the bioavailability of nb in soils are needed as well as general trends of its fate in the specific environment around th ...	2005	15511567
development of an expressed sequence tag (est) resource for wheat (triticum aestivum l.): est generation, unigene analysis, probe selection and bioinformatics for a 16,000-locus bin-delineated map.	this report describes the rationale, approaches, organization, and resource development leading to a large-scale deletion bin map of the hexaploid (2n = 6x = 42) wheat genome (triticum aestivum l.). accompanying reports in this issue detail results from chromosome bin-mapping of expressed sequence tags (ests) representing genes onto the seven homoeologous chromosome groups and a global analysis of the entire mapped wheat est data set. among the resources developed were the first extensive public ...	2004	15514037
construction and evaluation of cdna libraries for large-scale expressed sequence tag sequencing in wheat (triticum aestivum l.).	a total of 37 original cdna libraries and 9 derivative libraries enriched for rare sequences were produced from chinese spring wheat (triticum aestivum l.), five other hexaploid wheat genotypes (cheyenne, brevor, tam w101, bh1146, butte 86), tetraploid durum wheat (t. turgidum l.), diploid wheat (t. monococcum l.), and two other diploid members of the grass tribe triticeae (aegilops speltoides tausch and secale cereale l.). the emphasis in the choice of plant materials for library construction w ...	2004	15514038
chromosome bin map of expressed sequence tags in homoeologous group 1 of hexaploid wheat and homoeology with rice and arabidopsis.	a total of 944 expressed sequence tags (ests) generated 2212 est loci mapped to homoeologous group 1 chromosomes in hexaploid wheat (triticum aestivum l.). est deletion maps and the consensus map of group 1 chromosomes were constructed to show est distribution. est loci were unevenly distributed among chromosomes 1a, 1b, and 1d with 660, 826, and 726, respectively. the number of est loci was greater on the long arms than on the short arms for all three chromosomes. the distribution of ests along ...	2004	15514039
group 3 chromosome bin maps of wheat and their relationship to rice chromosome 1.	the focus of this study was to analyze the content, distribution, and comparative genome relationships of 996 chromosome bin-mapped expressed sequence tags (ests) accounting for 2266 restriction fragments (loci) on the homoeologous group 3 chromosomes of hexaploid wheat (triticum aestivum l.). of these loci, 634, 884, and 748 were mapped on chromosomes 3a, 3b, and 3d, respectively. the individual chromosome bin maps revealed bins with a high density of mapped ests in the distal region and bins o ...	2004	15514041
analysis of expressed sequence tag loci on wheat chromosome group 4.	a total of 1918 loci, detected by the hybridization of 938 expressed sequence tag unigenes (ests) from 26 triticeae cdna libraries, were mapped to wheat (triticum aestivum l.) homoeologous group 4 chromosomes using a set of deletion, ditelosomic, and nulli-tetrasomic lines. the 1918 est loci were not distributed uniformly among the three group 4 chromosomes; 41, 28, and 31% mapped to chromosomes 4a, 4b, and 4d, respectively. this pattern is in contrast to the cumulative results of est mapping in ...	2004	15514042
deletion mapping of homoeologous group 6-specific wheat expressed sequence tags.	to localize wheat (triticum aestivum l.) ests on chromosomes, 882 homoeologous group 6-specific ests were identified by physically mapping 7965 singletons from 37 cdna libraries on 146 chromosome, arm, and sub-arm aneuploid and deletion stocks. the 882 ests were physically mapped to 25 regions (bins) flanked by 23 deletion breakpoints. of the 5154 restriction fragments detected by 882 ests, 2043 (loci) were localized to group 6 chromosomes and 806 were mapped on other chromosome groups. the numb ...	2004	15514044
a chromosome bin map of 2148 expressed sequence tag loci of wheat homoeologous group 7.	the objectives of this study were to develop a high-density chromosome bin map of homoeologous group 7 in hexaploid wheat (triticum aestivum l.), to identify gene distribution in these chromosomes, and to perform comparative studies of wheat with rice and barley. we mapped 2148 loci from 919 est clones onto group 7 chromosomes of wheat. in the majority of cases the numbers of loci were significantly lower in the centromeric regions and tended to increase in the distal regions. the level of dupli ...	2004	15514045
a chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat.	because of the huge size of the common wheat (triticum aestivum l., 2n = 6x = 42, aabbdd) genome of 17,300 mb, sequencing and mapping of the expressed portion is a logical first step for gene discovery. here we report mapping of 7104 expressed sequence tag (est) unigenes by southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. each est detected a mean of 4.8 restriction fragments and 2.8 loci. more loci were mapped in the b genome (5774) than in th ...	2004	15514046
construction of a full-length cdna library from young spikelets of hexaploid wheat and its characterization by large-scale sequencing of expressed sequence tags.	the polyploid nature of wheat is a key characteristic of the plant. full-length complementary dnas (cdnas) provide essential information that can be used to annotate the genes and provide a functional analysis of these genes and their products. we constructed a full-length cdna library derived from young spikelets of common wheat, and obtained 24056 expressed sequence tags (ests) from both ends of the cdna clones. these ests were grouped into 3605 contigs using the phrap method, representing exp ...	2004	15514442
a rapid response of beta-amylase to nitric oxide but not gibberellin in wheat seeds during the early stage of germination.	the effects of nitric oxide (no) and gibberellic acid (ga(3)) on the responses of amylases in wheat (triticum aestivum l.) seeds (caryopses) were investigated during the first 12 h of germination. ga(3) had no effects on the activities of alpha-amylase (ec 3.2.1.1) or beta-amylase (ec 3.2.1.2), either in intact seeds or embryoless halves within 12 h. in contrast, addition of sodium nitroprusside (snp), an no donor, was able to induce a rapid increase in beta-amylase activity without affecting al ...	2005	15517355
effect of chemical amendments on the concentration of cadmium and lead in long-term contaminated soils.	the availability of metal in contaminated soil can be reduced by the addition of soil amendments. the objectives of this study are to study the effects of applying different soil amendments on the concentration of cd and pb in soil solution, dtpa or edta extractable cd and pb, and the uptake of cd and pb by wheat (triticum vulgare) when growing in long-term cd and pb-contaminated soils, more than 20 years. the soil amendments, including check, compost, zinc oxide, calcium carbonate, calcium carb ...	2004	15519390
fingerprinting of common wheat cultivars with an alw44i-based aflp method.	a simplified aflp method, based on methylation-sensitive alw44i restriction endonuclease, has been developed and evaluated for fingerprinting 15 wheat cultivars. the selected germplasms represented groups of spring and winter wheats with and without the 1bl.1rs translocation. ten selective primers yielded 57 markers, including 19 polymorphic bands. three markers (15.8%) were specific to wheat carrying the 1bl.1rs translocation, thus conflicting with the frequency expected by random marker distri ...	2004	15523150
immunochemical approach to the problem of differential determination of natural forms of abscisic acid.	an original modification of the standard elisa procedure for differential determination of different forms of abscisic acid (aba) is proposed. it is shown that endogenous forms of aba may be quantitatively determined in plant tissues subjected to minimal treatment, without purification of the hormones and their chemical modification. the modification has been approved when analyzing changes in the content of different aba forms in plant tissues differing in physiological activity. quantitative d ...	2004	15527409
selective transcriptional down-regulation of anther invertases precedes the failure of pollen development in water-stressed wheat.	water deficit during male meiosis in wheat (triticum aestivum l.) causes pollen sterility. with a view to identifying the internal trigger for this failure, it was found that water stress specifically impairs the activities of vacuolar and cell-wall invertases in anthers prior to the arrest of pollen development. the enzymes are affected only when water deficit occurs around meiosis. three invertase cdnas, two encoding the cell-wall (ivr1, ivr3) and one the vacuolar (ivr5) isoform, were isolated ...	2005	15533880
radionuclide transport above a near-surface water table: iv. soil migration and crop uptake of chlorine-36 and technetium-99, 1990 to 1993.	vertical distributions of (36)cl and (99)tc are presented from deep and shallow lysimeters above artificially controlled water tables for a 4-yr experiment from 1990 to 1993. activity concentration profiles were all measured in late summer when a winter wheat (triticum aestivum l. cv. pastiche) crop was harvested. after harvest, activity concentrations in different organs of the crop were determined and crop uptake quantified as both an inventory ratio (ir) and a transfer factor (tf(w)), weighte ...	2013	15537950
a biocatalyst for the removal of sulfite from alcoholic beverages.	the presence of sulfites in alcoholic beverages, particularly in wines, can cause allergic responses with symptoms ranging from mild gastrointestinal problems to life threatening anaphylactic shock in a substantial portion of the population. we have developed a simple and inexpensive biocatalytic method that employs wheatgrass (triticum aestivum) chloroplasts for the efficient oxidation of sulfites in wines to innocuous sulfates. a sufficiently high rate of sulfite oxidation was obtained in the ...	2005	15540199
sex ratios of sitodiplosis mosellana (diptera: cecidomyiidae): implications for pest management in wheat (poaceae).	sex ratios of populations of the wheat midge sitodiplosis mosellana gehin, developing on wheat triticum aestivum l., were determined at reproduction, adult emergence, and dispersal. the patterns of sex ratio through the life cycle of s. mosellana result from: (i) a genetic mechanism that causes all or nearly all of the progeny of individual females to be a single sex, with an overall sex ratio that is slightly biased at 54-57% females; (ii) a differential mortality during diapause that increases ...	2004	15541195
single and joint toxicity of chlorimuron-ethyl, cadmium, and copper acting on wheat triticum aestivum.	investigation of the toxicological effects of some agricultural pollutants on germination rate and on shoot and root elongation of wheat (triticum aestivum) was carried out. seeds of wheat were exposed to various concentrations of chlorimuron-ethyl with or without cadmium and copper addition. the inhibitory rates of seed germination and shoot and root elongation of wheat were calculated. significant linear relationships between the root and shoot elongation and the concentration of chlorimuron-e ...	2005	15546632
two quality-associated hmw glutenin subunits in a somatic hybrid line between triticum aestivum and agropyron elongatum.	high-molecular-weight glutenin subunits (hmw-gss) from hybrid line ii-12 between wheat (triticum aestivum l.) and agropyron elongatum (host) nivski were characterized with sds-page. out of these hmw-gss, two subunits, h1bx and h1by, had mobilities similar to the subunits 1bx13 and 1by16 from common wheat 4072, which was used as control. polyclonal antibodies (pabs) of h1bx and h1by were prepared, and western blotting showed that the pabs had strong affinities for h1bx and h1by, separately. the s ...	2004	15551037
detection and mapping of qtl for earliness components in a bread wheat recombinant inbred lines population.	earliness, an adaptative trait and factor of variation for agronomic characters, is a major trait in plant breeding. its constituent traits, photoperiod sensitivity (ps), vernalization requirement (vr) and intrinsic earliness (ie), are largely under independent genetic controls. mapping of major genes and quantitative trait loci (qtl) controlling these components is in progress. most of the studies focusing on earliness considered it as a whole or through one (or two) of its components. the purp ...	2004	15551039
[effect of salicylic acid on the activity of antioxidant enzymes in wheat under conditions of salination].	the effect of pretreatment with 0.05 mm salicylic acid (sa) on the activity of superoxide dismutase (sod) and peroxidase in the roots of four-day-old seedlings of wheat (triticum aestivum l.) was studied under conditions of salination. the level of the stress-induced accumulation of active oxygen species and, therefore, activities of sod and peroxidase in seedlings pretreated with sa were significantly lower than in untreated seedlings, which indicates that these enzymes contribute to the protec ...	2015	15553791
qtl analysis and comparative genomics of herbage quality traits in perennial ryegrass (lolium perenne l.).	genetic control of herbage quality variation was assessed through the use of the molecular marker-based reference genetic map of perennial ryegrass (lolium perenne l.). the restriction fragment length polymorphism (rflp), amplified fragment length polymorphism (aflp) and genomic dna-derived simple sequence repeat-based (ssr) framework marker set was enhanced, with rflp loci corresponding to genes for key enzymes involved in lignin biosynthesis and fructan metabolism. quality traits such as crude ...	2005	15558228
[the effect of lr19-translocation on in vitro androgenesis and inheritance of leaf-rust resistance in dh3 lines and f2 hybrids of common wheat].	leaf-rust resistance and androgenesis were studied in the anther cultures of triticum aestivum l., which included saratovskaya 29 cultivar, the isogenic line ps29, and three f1 hybrids (l503/s55, l504/s58, ats7/l1063) with 7ds-7dl-7ae#1l translocation of lr19 gene (lr19 translocation) from agropyron elongatum (host) p.b. the lr19 translocation was shown to affect the induction of embryogenesis and green plant regeneration. the frequencies of lr19 translocation differed in f2 hybrids obtained by ...	2004	15559150
[barley chromosome identification using genomic in situ hybridization in the genome of backcrossed progeny of barley-wheat amphiploids [h. geniculatum all. (2n = 28) x t. aestivum l. (2n = 42)] (2n = 70)].	genomic in situ hybridization (gish) has been used to study characteristics of the formation of alloplasmic lines detected among self-pollinated backcrossed progeny (bc1f5-bc1f8) of barley--wheat amphiploids [hordeum geniculatum all. (2n = 28) x triticum aestivum l. (2n = 42)] (2n = 70). the chromosome material of the wild barley h. geniculatum has been shown to contribute to these lines. for example, fifth-generation plants (bc1f5) had genotypes (2n = 42w + 2g), (2n = 42w + 1g + 1tg), and (2n = ...	2004	15559151
[resistance to fungal diseases in hybrid progeny from crosses between common wheat variety saratovskaia 29 and the amphidiploid triticum timopheevii/triticum tauschii (aaggdd)].	the progeny of bc6f2-bc9f(2)-4 has been analyzed for resistance to brown rust (lr genes) and powdery mildew (pm genes). this progeny was obtained due to introgression of the alien material from the synthetic hexaploid wheat triticum timopheevii/aegilops squarrosa (= triticum tauschii aaggdd, 2n = 42) into the common wheat variety saratovskaya 29. against the background of natural infection, the lines resistant to both diseases and to either of them were developed. the brown-rust and powdery-mild ...	2004	15559157
relationship between atpase activity and conjugated polyamines in mitochondrial membrane from wheat seedling roots under osmotic stress.	the effects of osmotic stress on the atpase activity, the contents of -sh group and conjugated polyamines in mitochondrial membrane from wheat seedling [triticum aestivum l. cv. yumai no. 18 (drought-tolerant) and cv. yumai no. 9 (drought-sensitive)] roots were investigated. the results showed that atpase activity and -sh group content decreased with polyethylene glycol(peg) 6000(-0.55 mpa) treatment for 7 d, in concert with the decrease of the ratio of noncovalently conjugated spermidine (ncc-s ...	2004	15559797
induction of cytoplasmic mannose-binding jacalin-related lectins is a common phenomenon in cereals treated with jasmonate methyl ester.	treatment of whole plants with jasmonic acid methyl ester induces lectin activity in leaves of oryza sativa, hordeum vulgare, triticum vulgare, secale cereale and zea mays. purification and characterization of the lectins revealed that they all have a very similar molecular structure and carbohydrate-binding properties. further analysis of the cdna clones encoding the lectins revealed that they all belong to the family of cytoplasmic mannose-specific jacalin-related lectins.	0	15560260
a nearest-neighboring-end algorithm for genetic mapping.	high-throughput methods are beginning to make possible the genotyping of thousands of loci in thousands of individuals, which could be useful for tightly associating phenotypes to candidate loci. current mapping algorithms cannot handle so many data without building hierarchies of framework maps.	2005	15564296
new taxi-type xylanase inhibitor genes are inducible by pathogens and wounding in hexaploid wheat.	taxi-i (triticum aestivum xylanase inhibitor i) is a wheat grain protein that inhibits arabinoxylan fragmentation by microbial endo-beta-1,4-xylanases used in the food industry. although taxi was speculated to be involved in counterattack against pathogens, there is actually no evidence to support this hypothesis. we have now demonstrated the presence of taxi family members with isolation of two mrna species, taxi-iii and taxi-iv. at the nucleotide sequence level, taxi-iii and taxi-iv were 91.7% ...	2004	15564518
the relation of starch phosphorylases to starch metabolism in wheat.	tissues of wheat (triticum aestivum l., var. star) exhibit three starch phosphorylase activity forms resolved by non-denaturing polyacrylamide gel affinity electrophoresis (p1, p2 and p3). compartmentation analysis of young leaf tissues showed that p3 is plastidic, whereas p1 and p2 are cytosolic. p1 exhibits a strong binding affinity to immobilized glycogen upon electrophoresis, whereas p2 and the chloroplastic p3 do not. cytosolic leaf phosphorylase was purified to homogeneity by affinity chro ...	2004	15564531
drought controls on h2o2 accumulation, catalase (cat) activity and cat gene expression in wheat.	plants co-ordinate information derived from many diverse external and internal signals to ensure appropriate control of gene expression under optimal and stress conditions. in this work, the relationships between catalase (cat) and h2o2 during drought in wheat (triticum aestivum l.) are studied. drought-induced h2o2 accumulation correlated with decreases in soil water content and co2 assimilation. leaf h2o2 content increased even though total cat activity doubled under severe drought conditions. ...	2005	15569704
use of the mobile nylon bag technique to determine the digestible energy content of traditional and non-traditional feeds for swine.	the following experiment was conducted using the mobile nylon bag technique (mnbt) to determine dry matter and energy digestibility in traditional feeds as well as non-traditional feeds in order to calculate digestible energy (de) values for use in ration formulation programmes. a total of 22 ingredients were tested in this experiment including the traditional cereal grains barley, corn, oats and wheat, as well as secondary cereal grains such as normal and low viscosity rye, low lignin and high ...	2004	15570743
[analysis of intraspecific divergence of hexaploid wheat triticum spelta l. by chromosome c-banding].	intraspecific divergence of hexaploid wheat triticum spelta was studied by chromosome c-banding in 41 accessions of different geographic origins. the spelt accessions did not differ in karyotype structure or heterochromatin distribution from common wheat, but showed greater intraspecific polymorphism for chromosome rearrangements (translocations, inversions) and banding patterns. on evidence of c-banding patterns, spelt was assumed to occupy an intermediate position between tetraploid and hexapl ...	2004	15575503
a reverse genetic, nontransgenic approach to wheat crop improvement by tilling.	we report the use of tilling (targeting induced local lesions in genomes), a reverse genetic, nontransgenic method, to improve a quality trait in a polyploid crop plant. waxy starches, composed mostly of amylopectin, have unique physiochemical properties. wheat with only one or two functional waxy genes (granule-bound starch synthase i, or gbssi) produces starch with intermediate levels of amylopectin. we have identified 246 alleles of the waxy genes by tilling each homoeolog in 1,920 allohexapl ...	2005	15580263
ftir imaging of wheat endosperm cell walls in situ reveals compositional and architectural heterogeneity related to grain hardness.	endosperm cell walls of cultivars of wheat (triticum aestivum l.) selected for their endosperm texture (two soft and two hard) were analysed in situ by fourier transform infrared (ftir) microspectroscopy. ftir imaging coupled with statistical analysis was used to map the compositional and structural heterogeneity within transverse sections from which cell contents had been removed by sonication. in the majority of grains analysed, two distinct populations of endosperm cells could be identified b ...	2005	15580525
marker-assisted selection for leaf rust resistance genes lr19 and lr24 in wheat (triticum aestivum l.).	leaf rust caused by puccinia recondita f.sp. tritici is a wheat disease of worldwide importance. wheat genotypes known to carry specific rust resistance genes and segregating lines that originated from various cross combinations and derived from distinct f2 lineage, so as to represent a diverse genetic background, were included in the present study for validation of molecular markers for lr19 and lr24. sts markers detected the presence of the leaf rust resistance gene lr19 in a thatcher nil (tc* ...	2004	15586436
preferential expression of a hlp homolog encoding a mitochondrial l14 ribosomal protein in stamens of common wheat.	interaction between nucleus and cytoplasm has essential roles in plant development, including that of floral organs. we isolated a wheat homolog whlp of arabidopsis huellenlos paralog (hlp) gene encoding a mitochondrial (mt) ribosomal protein l14. transient expression analysis using the green fluorescent protein (gfp) fusion protein showed that 50 amino residues located on the n-terminal of the wheat hlp homolog (whlp) protein acted as a mt-targeting signal (mts). expression patterns of the whlp ...	2004	15588583
two point mutations identified in emmer wheat generate null wx-a1 alleles.	in this report, the wx-a1 mutations carried by a triticum dicoccoides line from israel and a triticum dicoccum line from yugoslavia are characterized. a single nucleotide insertion in the t. dicoccoides null allele and a single nucleotide deletion in the t. dicoccum null allele each cause frameshift mutations that induce premature termination codons more than 55 nucleotides upstream of the last exon-exon junction. in both mutants, wx-a1 transcripts were detectable in 10 day post-anthesis endospe ...	2005	15592661
novel mode of resistance to fusarium infection by a mild dose pre-exposure of cadmium in wheat.	exposure of healthy wheat seeds (triticum aestivum var sonalika) to mild dose of cadmium (cd(2+)) given as 50 microm cdcl(2) for 48 h and then washed off cd(2+) offered resistance to the subsequent infection by fusarium oxysporum inoculum. seven days old seedlings having two primary leaves were aseptically inoculated with fungus, f. oxysporum (1 x 10(6)) spores. the seedlings pre-exposed to low level of cd(2+) survived the fusarium infection, while plantlets without cd(2+) stress wilted and then ...	2004	15596097
[exogenous nitric oxide alleviates osmotic stress-induced membrane lipid peroxidation in wheat seedling leaves].	when wheat (triticum aestivum l. yangmai 158) seedling (with three fully expanded leaves) roots were treated with 15% peg-6000 in combination with different concentrations (0.1 and 0.5 mmol/l) of exogenous nitric oxide donor sodium nitroprusside (snp) and no(-)(3)/no(-)(2) (control), the enhancement of lipoxygenase (lox) activity in wheat seedling leaves under osmotic stress was delayed at the lower concentration of snp treatment (0.1 mmol/l), while the generation rate of o(-.)(2), the enhanceme ...	2004	15599047
chloroplast and nuclear dna variation in common wheat: insight into the origin and evolution of common wheat.	to understand the origin and evolution of common wheat, chloroplast (ct) and nuclear dna variations were studied in five hexaploid and three tetraploid wheat subspecies. based on chloroplast simple sequence repeats at 24 loci, they were classified into two major plastogroups. plastogroup i consisted of 11 plastotypes, including the major plastotype h10 that occurred at the highest frequency (59%) in common wheat. plastogroup ii consisted of five plastotypes and occurred in eight out of 27 access ...	2004	15599057
a mini exon in the sucrose:sucrose 1-fructosyltransferase gene of wheat.	we previously reported the cloning of a wheat sucrose:sucrose 1-fructosyltransferase (1-sst) cdna, designated wft2. wft2 proteins have fructosyltransferase enzyme activity and initiate fructan synthesis (biosci. biotechnol. biochem. 66 (2002) 2297). in the current study, we cloned a genomic dna fragment carrying the full-length 1-sst gene from winter wheat (triticum aestivum). the genomic 1-sst gene is 3326 bp in length and contains four exons and three introns. exon 2 has only 9 bp. this sequen ...	2004	15602819
a genetic and structural analysis of the n-glycosylation capabilities.	the recent draft sequencing of the rice (oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. in this study, we have not only identified genes putatively encoding enzymes involved in n-glycosylation, but have examined by maldi-tof ms the structures of the n-glycans of rice and other monocotyledons (maize, wheat and dates; zea mays, triticum aestivum and phoenix dactylifera); these data show tha ...	2004	15604706
real time rt-pcr and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins.	developing seeds from triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by real time rt-pcr. mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.	2004	15604827
phytoecdysteroids: a novel defense against plant-parasitic nematodes.	the phytoecdysteroid, 20-hydroxyecdysone (20e), is a major molting hormone of invertebrates, possibly including nematodes. as 20e is inducible in spinach, the defensive role against plant-parasitic nematodes was investigated. the effects of direct application on nematodes was assessed by treating cereal cyst nematode, heterodera avenae, juveniles with concentrations of 20e from 8.2 x 10(-8) to 5.2 x 10(-5) m before applying to triticum aestivum growing in sand. h. avenae, heterodera schachtii (s ...	2004	15609826
[effects of cytokinin preparations on the stability of the photosynthetic apparatus of two wheat cultivars under water deficiency].	carboxylase activities of the key enzyme of carbon metabolism, ribulose-bisphosphate carboxylase/oxygenase (rubisco; ec 4.1.1.39), and phosphoenolpyruvate carboxylase (pepc; ec 4.1.1.31), as well as intensities of carbon dioxide photosynthetic assimilation in young seedlings and adult leaves of the wheat triticum aestivum l. cultivars mironovskaya 808 (a more tolerant) and lyutestsens 758 (a less tolerant), were compared under conditions of progressive water deficiency. the water stress had more ...	2013	15609857
[change in lectin specificity of winter wheat seedlings in the course of infection with mycoplasms].	the activity of soluble lectins in leaves and roots of seedlings of winter wheat (triticum aestivum l.) cultivar mironovskaya 808 increased 1 day and 2 days, respectively, after infection with the mycoplasma acholeplasma laidlawii 118. analysis of acid-soluble proteins of wheat leaves by page revealed the appearance of 22- and 20-kda polypeptides, the disappearance of a 14-kda polypeptide, and an increase in the content of polypeptides with molecular weights of 76, 48, 25, and 18 kda. the 18-kda ...	2004	15609859
[revealing hereditary variation of winter hardiness in cereals].	a set of cereal crops and differentiating cultivars was shown to be of utility for identifying the major abiotic factors that limit the survival of winter crops in the cold season of a particular year. with this approach, the season was identified (1997-1998, belgorod) when the survival of cereals depended on the tolerance to anaerobiosis rather than on the frost resistance. differentiation of common wheat cultivars with respect to this property was attributed to a locus designated win1 (winter ...	0	15612569
[trends in genetic diversity change of spring bread wheat cultivars released in russia in 1929-2003].	using genealogy analysis, we studied genetic diversity of 340 cultivars of spring bread wheat that were released on the territory of russia in 1929-2003. trends in the temporal change of genetic diversity were inferred from analysis of a set of n x m matrices, where n is the number of the released cultivars and m is the number of original ancestors. the pool of original ancestors of the spring bread wheat cultivars for the total period of study included 255 landraces, of which 88 were from the f ...	2004	15612570
agropyron elongatum chromatin localization on the wheat chromosomes in an introgression line.	the introgressed small-chromosome segment of agropyron elongatum (host.) neviski (thinopyrum ponticum podp.) in f5 line ii-1-3 of somatic hybrid between common wheat (triticum aestivum l.) and a. elongatum was localized by sequential fluorescence in situ hybridization (fish), genomic in situ hybridization (gish) and karyotype data. karyotype analysis offered basic data of arm ratios and relative lengths of 21 pairs of chromosomes in parent wheat jinan177 and hybrid ii-1-3. using special high rep ...	2005	15616822
complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis.	to examine the role of isoamylase1 (isa1) in amylopectin biosynthesis in plants, a genomic dna fragment from aegilops tauschii was introduced into the isa1-deficient rice (oryza sativa) sugary-1 mutant line em914, in which endosperm starch is completely replaced by phytoglycogen. a. tauschii is the d genome donor of wheat (triticum aestivum), and the introduced fragment effectively included the gene for isa1 for wheat (taisa1) that was encoded on the d genome. in taisa1-expressing rice endosperm ...	2005	15618430
[resolving capacity of monosomic line analysis in cytogenetic studies of common wheat].	basing on statistic analysis of the character of homologue pairing in the series of monosomic lines of wheat milturum 553 it was found that the diversions of monosomic and disomic plants from their original parent were caused by the changes in the system of actions and interrelations of genes caused by hemizygous state of chromosomes. resolving capacity of monosomic line analysis as a method of cytogenetic investigations of wheat was demonstrated. input of each chromosome in determination of mei ...	2008	15619985
transfer of small chromosome fragments of agropyron elongatum to wheat chromosome via asymmetric somatic hybridization.	the chromosome constitution of hybrids and chromatin patterns of agropyron elongatum (host)neviski in f5 somatic hybrid lines ii -1-3 and i-1-9 between triticum aestivum l. and a. elongatum were analyzed. based on the statistic data of pollen mother cells, f5 i-1-9 and ii-1-3 had 20-21 bivalents with a frequency of 84.66% and 85.28%, of which, 89.83% and 89.57% were ring bivalents. the result indicated that both hybrid lines were basically stable in the chromosome constitution and behavior. rapd ...	2004	15623155
[research advances in wheat (triticum aestivum) allelopathy].	wheat (triticum aestivum) is the main food crop in the world, and plays an important role in agricultural production. in order to enhance wheat yield, herbicides and germicides were intensively applied and made negative effects on the environment. wheat possesses allelopathic potential for weed suppression and disease control through the release of secondary metabolites from its living plants or residues, which could avoid the environment pollution brought by herbicides and germicides. this pape ...	2004	15624846
imidazolinone-tolerant crops: history, current status and future.	imidazolinone herbicides, which include imazapyr, imazapic, imazethapyr, imazamox, imazamethabenz and imazaquin, control weeds by inhibiting the enzyme acetohydroxyacid synthase (ahas), also called acetolactate synthase (als). ahas is a critical enzyme for the biosynthesis of branched-chain amino acids in plants. several variant ahas genes conferring imidazolinone tolerance were discovered in plants through mutagenesis and selection, and were used to create imidazolinone-tolerant maize (zea mays ...	2005	15627242
[nuclear and cytoplasmic genome analysis of somatic hybrid of triticum aestivum l. and leymus chinensis (trin.) tzvel].	intergeneric somatic hybrids were obtained by fusion between protoplasts of triticum aestivum l. cv. jinan 177 and leymus chinensis (trin.) tzvel. protoplasts of l. chinensis were exposed to uv (300 microw/cm(2)) for 30 s, 45 s and 1 min before fusion. the results of morphological and chromosomal observation, isozyme pattern as well as rapd analysis and the 5s rdna space sequence analysis showed the hybrid nature of the regenerated colonies of fusion combination t (+) l (uv 30 s). restriction fr ...	2004	15627685
cloning and characteristics of an allene oxide synthase gene (taaos) of winter wheat.	allene oxide synthase (aos) is the first enzyme in the lipoxygenase pathway which leads to the formation of jasmonic acid (ja). a full length cdna of taaos was cloned in winter wheat (triticum aestivum l. cv. jinghua no.3) seedlings. the open reading frame encompassed 1410 bp encoding a polypeptide of 470 amino acids with calculated molecular mass of 51.9 kd. southern blot analysis suggested there are three copies of the gene in wheat genome. the taaos mrna could be strongly induced by exogenous ...	2004	15627690
[effects of nitric oxide on root growth and its oxidative damage in wheat seedling under salt stress].	effects of nitric oxide (no), a substance newly found to have protective functions in plants, on root growth of wheat (triticum aestivum l. yangmai 158) seedlings under salt stress were studied. sodium nitroprusside (snp), an no donor, markedly alleviated the inhibitory effect of salt on root elongation at salt concentrations around 150 mmol/l, but was ineffective when nacl concentration was at 300 mmol/l or higher. it was most effective at 0.05-0.1 mmol/l, and had harmful effect at 0.30-5 mmol/ ...	2004	15627712
[physiological effects of taurine on the growth of wheat (triticum aestivum l.) seedlings].	wheat (triticum aestivum l.) seedlings were grown in taurine solution at concentrations of 10, 100, 500, 1000 and 5000 mg/l, and the photochemistry efficiency, the relative permeability of membrane, membrane lipid peroxidation and the growth indexes in wheat seedlings were determined. the results showed that taurine treatments distinctly promoted the growth of wheat seedlings and increased root length, plant height, dry weight and fresh weight single plant of wheat seedlings. in addition, the ph ...	2004	15627716
identification of oligopeptidase b in higher plants. purification and characterization of oligopeptidase b from quiescent wheat embryo, triticum aestivum.	proteolytic enzymes in general, and cysteine proteases in particular, play key roles in seed germination and early seedling growth. however, the precise mechanism by which the serine proteases are regulated remains unclear. trypsin-like activity was detected in wheat germ (quiescent embryo) and this activity increased in the germinating embryo. in this work, a trypsin-like serine protease expressed in wheat germ was purified to homogeneity by chromatography through deae-cellulose, phenyl-sepharo ...	2004	15632308
[rapd marker analysis of continuous divergent selection in cross-bred population of wheat (triticum aestivum l.)].	a base population was established through multi-parent random crossing by using taigu dominant male-sterile wheat, and then five cycles of 2-way selection for four quantitative characters were conducted. the dynamic changes of genetic structures in the open-pollinated wheat population were examined by rapd technique. seven primers in rapd analysis amplified 116 sites. the results of gene frequencies and phenotypic bands showed abundant genetic variations existed in the population. the percentage ...	2003	15639875
molecular characterization of a gene encoding n-myristoyl transferase (nmt) from triticum aestivum (bread wheat).	myristoyl-coa:protein n-myristoyl transferase (nmt; ec 2.3.1.97) acylates the gly residue abutting the n-terminal met with a myristic acid following the removal of the met residue in certain eukaryotic proteins, and in some cases myristoylation is essential to cell growth and survival. we report the cloning of a full-length cdna encoding nmt from triticum aestivum (tanmt). the cdna included a predicted open reading frame of 1317 nucleotides, which encoded a predicted protein of 438 amino acids c ...	2004	15644961
high level of genetic diversity among spelt germplasm revealed by microsatellite markers.	the genetic diversity of spelt (triticum aestivum (l.) thell. subsp. spelta (l.) thell.) cultivated presently is very narrow. although the germplasm collections of spelt are extensive, the related genetic knowledge is often lacking and makes their use for genetic improvement difficult. the genetic diversity and structure of the spelt gene pool held in gene banks was determined using 19 simple sequence repeat (ssr) markers applied to 170 spelt accessions collected from 27 countries and 4 continen ...	2004	15644962
genetic mapping of three alleles at the pm3 locus conferring powdery mildew resistance in common wheat (triticum aestivum l.).	a set of differential isolates of blumeria graminis f.sp. tritici was used to identify 10 alleles at the pm3 locus on the short arm of chromosome 1a. three f3 populations were used to map pm3h in abessi, pm3i in line n324, and pm3j alleles in gus 122 relative to microsatellite markers. in total, 13 marker loci were mapped on chromosome 1as and 1 marker on 1al. the order of marker loci in the 3 mapping populations is consistent with previously published maps. all 3 alleles were mapped in the dist ...	2004	15644971
combined use of linked markers for genotyping the pm1 locus in common wheat.	genotyping of 98 wheat cultivars/lines was carried out with molecular markers that are linked to the pm1 locus: two bi-allelic (dominant) markers: the sequence-tagged site xsts638-7a and the amplified fragment length polymorphism xe39m58-77-7a; and the multi-allelic simple sequence repeat marker xgwm344-7a. employing segregation data recorded in the population chinese spring x virest (pm1e), genetic mapping revealed that xgwm344-7a and xe39m58-77-7a were distally linked to pm1e in the repulsion ...	2004	15647799
development of the single nucleotide polymorphism marker of the wheat lr1 leaf rust resistance gene.	the range of publicly available data on plant nucleotide sequences opens a new possibility in the design of snp assays. the purpose of this study was to identify point mutations in genomic sequences closely linked to the lr1 leaf rust resistance gene, and to develop snp markers based on primer extension (snupe) facilitating efficient marker-based selection procedures, e.g. the pyramiding of resistance genes. studies were performed on the panel of 37 wheat cultivars, the set of 41 thatcher near-i ...	2004	15647804
[detection of the introgression of genome elements of the aegilops cylindrica host. into the triticum aestivum l. genome by issr and ssr analysis].	to reveal sites of the donor genome in wheat crossed with aegilops cylindrica, which acquired conferred resistance to fungal diseases, a comparative analysis of introgressive and parental forms was conducted. two systems of pcr analysis, issr and ssr-pcr, were employed. upon use of 7 issr primers in genotypes of 30 individual plants bc1 f9 belonging to lines 5/55-91 and 5/20-91, 19 issr loci were revealed and assigned to introgressive fragments of aegilops cylindrica genome in triticum aestivum. ...	2004	15648148
[effect of an introgression from aegilops cylindrica host on manifestation of productivity traits in winter common wheat f2 plants].	the effect of introgression of a chromosome 1d segment from aegilops cylindrica to winter common wheat on productivity traits in f2 plants was studied using storage protein loci as genetic markers. an allele of the gliadin-coding gli-d1 locus served as a marker of the introgression. using of two- and three-locus interaction models, it was shown that the introgression tagged with gli-d1 affected the manifestation of productivity traits (productive tillering, grain weight per plant and grain numbe ...	2004	15648149
[molecular study and c-banding of chromosomes in common wheat alloplasmic lines obtained from the backcross progeny of barley-wheat hybrids hordeum vulgare l. (2n = 14) x triticum aestivum l. (2n = 42) and differing in fertility].	we studied common wheat alloplasmic lines differing in fertility traits, which had been obtained from the backcross progeny of barley-wheat hybrids hordeum vulgare l. (2n = 14) x triticum aestivum l. (2n = 42), using molecular analysis and chromosome c-banding. it was found that the nuclei of all alloplasmic lines studied, regardless of their fertility traits, contained only the common wheat chromosomes (2n = 42). the formation of line l-79(10)(3)f6, stable for self-fertility, from line l-79(10) ...	2004	15648150
characterization of est-derived microsatellites in the wheat genome and development of essr markers.	est-derived microsatellites or simple sequence repeats (essr) occur in expressed sequence tags (est). here we report characteristics of essrs in the wheat genome, construction of consensus chromosome bin maps of ssr-containing ests ((ssr)ests), and development of essr markers for the 21 wheat chromosomes. a perl script known as misa was used to identify essrs in wheat ests available in the database http://wheat.pw.usda.gov/cgi-bin/ace/search/west ). among 492,832 ests from the database, 36,520 ( ...	2005	15650880