Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| evidence for a developmentally regulated neurotrophic activity from chick retina affecting the survival of retinal ganglion cells from newborn rats. | the effect of conditioned medium from aggregates of chick embryo retinal cells was tested on the in vitro survival of retinal ganglion cells from newborn rats. ganglion cells were identified by the detection of retrogradely transported horseradish peroxidase injected bilaterally into the superior colliculus. culture medium conditioned with chick embryo retinae was tested on monolayers of rat retinal cells with plating densities ranging from 0.5 to 4 x 10(5) cells/cm2. in all cases the conditione ... | 1992 | 1342620 |
| [the localization of neurons in the nodose ganglion of the cat that innervate the rostral portion of the duodenum]. | the method of axonal transport of horseradish peroxidase was used for studying the sites of localization and morphological features of sensory neurons of the vagus nerve in the left and right ganglion nodosum innervating the rostral part of the duodenum. animals under nembutal narcosis were given 90-100 mcl of 33% solution of horseradish peroxidase ("olaine", riga rz 2.7) injected into a limited area (1 x 1 sm) of the muscular layer of the duodenum. horseradish peroxidase labelled neurons were f ... | 1992 | 1343239 |
| evidence for dorsal root projection to somatostatin-immunoreactive structures in laminae i-ii of the spinal dorsal horn. | in order to determine if somatostatin (som)-immunoreactive (i) cell bodies and processes in lamina (l) ii of the rat spinal cord receive dorsal root input, the latter were anterogradely labeled by wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp). som-i structures were demonstrated by immunohistochemistry. cell bodies labeled transscellularly or transsynaptically by wga-hrp and immunohistochemically for som were present in l ii. in addition, a l i cell was double labeled. these r ... | 1992 | 1347248 |
| converging gaba- and glutamate-immunoreactive axons make synaptic contact with identified hypothalamic neurosecretory neurons. | to study the neurochemical identity of axons in synaptic contact with identified hypothalamic neurosecretory neurons in rats, we combined retrograde axonal transport of a marker molecule with postembedding immunogold staining for amino acid neurotransmitters. after intravenous injections of horseradish peroxidase, neurosecretory neurons with axons in the median eminence or neurohypophysis transported the peroxidase retrogradely back to the cell body of origin. serial ultrathin sections from the ... | 1992 | 1349310 |
| immunoblot analysis of igg subclass antibody response against coxiella burnetii in balb/c mice. | balb/c mice were inoculated with live or inactivated organisms of coxiella burnetii, strain nine mile, phase i. sera collected after different time intervals were subjected to immunoblot analysis and results compared with elisa values. immunoblots were performed with different horseradish peroxidase labelled conjugates (goat anti-mouse igg1, igg2a, igg2b, igg3) and results monitored and analysed by laser densitometry. elisa analysis was performed using the same peroxidase labelled goat anti-mous ... | 1992 | 1350177 |
| corneal epithelial glycoproteins exhibit pseudomonas aeruginosa pilus binding activity. | adherence of pseudomonas aeruginosa to the cornea is a requisite step in the pathogenesis of bacteria-induced corneal disease. p. aeruginosa is capable of attaching to host epithelial cells by its pili, but there is little information regarding the epithelial receptors of this adhesin in the cornea. using nitro-cellulose blotting of polyacrylamide gels of solubilized adult mouse corneal epithelium, four major proteins (molecular weights: 38, 42, 57, and 66 kd) and several minor proteins were ide ... | 1992 | 1351476 |
| localization and soma diameter of rat gluteus medius motoneurons. | 1. retrograde transport of horseradish peroxidase was used to examine the localization and soma diameter of motoneurons innervating the deep (predominance of oxidative fibers) and superficial (predominance of non-oxidative fibers) portions of the gluteus medius muscle in rats. 2. the motoneurons innervating the deep portion were located caudally within the gluteus medius motoneuron pool and had smaller average soma diameter than that of motoneurons innervating the superficial portion. 3. these r ... | 1992 | 1351806 |
| the origin of brainstem noradrenergic and serotonergic projections to the spinal cord dorsal horn in the rat. | although it has been proposed that the locus coeruleus is the predominant, if not exclusive, brainstem origin of the noradrenergic innervation of the spinal dorsal horn, pharmacological studies argue otherwise. in this study we made localized injections of the retrograde tracer wheatgerm agglutinin conjugated to apo-horseradish peroxidase gold (wga:apohrp-au), in conjunction with immunocytochemical labeling for tyrosine hydroxylase (th) or serotonin (5-ht), to identify the brainstem source of th ... | 1992 | 1354402 |
| dopamine receptor antagonists increase markedly the quantity of retrograde transport of hrp in the rat masseteric motoneuron. | horseradish peroxidase (hrp) was injected, bilaterally, into the rat masseter muscle, subsequent to an intramuscular or intraperitoneal injection of one of five dopamine antagonists (chlorpromazine and haloperidol as the d1 and d2 receptor antagonist, sch 23390 as the specific d1 receptor antagonist, sulpiride and domperidone as the specific d2 receptor antagonist). control rats received an injection of a corresponding vehicle solution. after a survival period of 16 h, the brainstem was cut into ... | 1992 | 1355002 |
| projection from the ventrolateral medullary neurons containing tyrosine hydroxylase to the central amygdaloid nucleus in the rat. | by using a double-labeling produce of retrograde horseradish peroxidase (hrp) tracing and the immunocytochemical localization of tyrosine hydroxylase (th), the present study ascertained that the axonal fibers of catecholaminergic neurons in the caudal ventrolateral medulla projected to the central amygdaloid nucleus in the rat. the majority of double-labeled cells were observed primarily around the level of the obex. 92% of hrp retrogradely labeled cells contained th-like immunoreactive (th-ir), ... | 1992 | 1358401 |
| construction of a quadroma to alpha-endorphin/horseradish peroxidase using an actinomycin d-resistant mouse myeloma cell line. | a hybrid hybridoma (quadroma), secreting antibodies with double specificity to alpha-endorphin (alpha-ep) and horseradish peroxidase (hrp), has been produced. the bispecific antibodies constituted about 28-29% of all immunologically active igg, produced by quadroma. the quadroma was isolated by fusion of two mouse hybridomas (anti-hrp and anti-alpha-ep) with distinct phenotypes: double mutant amdr/hat(s), and wild type (amds/hatr). a novel strategy for the construction of a double-mutant was app ... | 1992 | 1358818 |
| amplified assay of alkaline phosphatase using flavin-adenine dinucleotide phosphate as substrate. | a simple to use, robust, quantitative, and extremely sensitive colorimetric assay for alkaline phosphatase (ec 3.1.3.1), designed to be used as a detection system in diagnostic assays employing antibodies or gene probes, is described. this technology is based on the novel principle of prosthetogenesis, according to which a purpose-designed substrate (a prosthetogen) for a primary analyte-linked enzyme label is hydrolyzed to produce a prosthetic group for a detector enzyme system. the prosthetoge ... | 1992 | 1360771 |
| response of oligodendrocytes to glutamate and gamma-aminobutyric acid in the intact mouse optic nerve. | the electrophysiological response to glutamate and gamma-aminobutyric acid (gaba) is determined in oligodendrocytes of the isolated intact mouse optic nerve, identified by their characteristic morphology following iontophoretic injection with horseradish peroxidase (hrp). in this study, mature myelin-forming oligodendrocytes are shown for the first time to respond to glutamate and gaba in situ, by a 2-3 mv depolarization. morphologically homogeneous oligodendrocytes exhibit a heterogeneous respo ... | 1992 | 1361974 |
| k-dependent inhibition in the dentate-ca3 network of guinea pig hippocampal slices. | 1. the occurrence of potassium-dependent inhibitory postsynaptic potentials (k-ipsps) in relation to burst discharges induced by 4-aminopyridine (4-ap; 30 microm) was studied in ca3, granule and hilar neurons in guinea pig hippocampal slices with the use of paired extra- and/or intracellular recording. 2. slow small (2-5 mv) and large (up to 30 mv) k-ipsps were observed in ca3, granule and in some hilar neurons during 4-ap applications in the presence of blockers for fast synaptic transmission, ... | 1992 | 1362214 |
| induction of interferon synthesis and cytotoxicity by murine peritoneal macrophages exposed to glycoprotein ligands. | thioglycollate-induced murine c57bl/6 and c3h/hen peritoneal macrophages synthesized interferon-beta (ifn-beta) in response to exposure to glycoproteins such as horseradish peroxidase (hrp) or mannosyl or fucosyl bovine serum albumin (bsaman of bsafuc, respectively), but not glucosylated or galactosylated bsa (bsaglu or bsagal, respectively). these results suggest participation of the mannosyl-fucosyl receptor (mfr) in this response. ifn synthesis was augmented by culturing macrophages in l cell ... | 1992 | 1362322 |
| direct observations of synapses between l-glutamate-immunoreactive boutons and identified spinocervical tract neurones in the spinal cord of the cat. | four spinocervical tract cells in lumbosacral spinal cords of adult cats were physiologically characterized and intracellularly labelled with horseradish peroxidase. the neurones were examined with a light microscope and reconstructed. selected regions were chosen for ultrastructural analysis. thin sections were treated to reveal the presence of l-glutamate by using the postembedding immunogold method. two antisera, which specifically recognise the presence of fixed glutamate in tissue, were use ... | 1992 | 1362431 |
| metabolism of 3-tert-butyl-4-hydroxyanisole by horseradish peroxidase and hydrogen peroxide. | 3-tert-butyl-4-hydroxyanisole (3-bha) was metabolized in the presence of horseradish peroxidase and hydrogen peroxide to 2-tert-butyl-p-benzoquinone (tbq), 2,3-epoxy-5-tert-butyl-1,4-benzoquinone (tbq-epoxide), and two known dimers. the formation of tbq from 3-bha required both horseradish peroxidase and hydrogen peroxide. when 2.5 mm 3-bha was incubated with horseradish peroxidase and hydrogen peroxide, the formation of tbq increased with hydrogen peroxide concentration up to 5 mm and decreased ... | 2013 | 1362932 |
| lectin staining studies on follicular atresia in house rat (rattus rattus). | topographic distribution of terminal and intercalary carbohydrate moieties were studied using horseradish peroxidase conjugated glycine max (gma), arachis hypogea (pna), lotus tetragonolobus (lta), bandirarea simplicifolia (bsa), and dolichos biflorus (dba) agglutinins. n-gal nac, alpha-d-gal, alpha-l-fuc, d-gal (beta-1-3)-d-gal nac, and alpha-beta-d gal nac linked glyco-conjugates were exposed during follicular atresia in oocyte, zona pellucida and granulosa cells. theca interna and interstitia ... | 1992 | 1365769 |
| influence of the reaction medium on the product distribution of peroxidase-catalysed oxidation of p-cresol. | p-cresol was oxidized by hydrogen peroxide in a reaction catalysed by horseradish peroxidase and the low molecular weight products were investigated. in aqueous media pummerer's ketone (i) was the dominating product but in organic media the product distribution was quite different; 2,2'-dihydroxy-5,5'-dimethyldiphenyl (ii) was the main low molecular weight product. similar product distributions were obtained with peroxidase adsorbed on a solid support and suspended in toluene and with peroxidase ... | 1990 | 1366748 |
| block copolymer microdomains: a novel medium for enzymatic reactions. | block copolymers exhibit the phenomenon of microdomain formation in pure states as well as in solutions. the microdomains vest the block copolymer assemblies with the intriguing characteristics of microheterogeneous media. we demonstrate that this microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. examples involving cholesterol oxidase and horseradish peroxidase are provided here. the observed chan ... | 2016 | 1367345 |
| micropatterning proteins and synthetic peptides on solid supports: a novel application for microelectronics fabrication technology. | in this paper, we describe a method for immobilizing proteins and synthesizing peptides in micrometer-dimension patterns on solid supports. microelectronics fabrication technology was adapted and used to lithographically direct the location of immobilization of proteins on appropriately derivatized surfaces. as examples, we micropatterned the protein bovine serum albumin (bsa) and the enzyme horseradish peroxidase (hrp). the catalytic activity of hrp was shown to be retained after being cross-li ... | 1992 | 1368007 |
| purification of glycoproteins by selective transport using concanavalin-mediated reverse micellar extraction. | a novel methodology for coupling liquid-liquid extraction with affinity interaction has been developed to selectively and efficiently purify and separate glycoproteins. the basis for the separation is the selective extraction of glycoproteins from an aqueous solution into a reverse micellar organic phase by using concanavalin a (a sugar-binding lectin) as a facilitative carrier. specifically, horseradish peroxidase (a common glycoprotein) can be bound to concanavalin a in an aqueous phase and th ... | 2009 | 1370045 |
| regeneration of the eighth cranial nerve in the bullfrog, rana catesbeiana. | the present study was done in order to document the ability of the eighth cranial nerve of the bullfrog (rana catesbeiana) to regenerate, the anatomic characteristics of the regenerated fibers, and the specificity of projections from individual endorgan branches of the nerve. the eighth cranial nerve was sharply transected between the ganglion cells and the brain stem in 40 healthy bullfrogs and allowed to regenerate. anatomic studies were performed in these animals a minimum of 3 months postope ... | 1992 | 1370219 |
| distribution of the blood-brain barrier in heterotopic brain transplants and its relationship to the lesions of eae. | the blood-brain barrier (bbb) is recognized as a barrier to the trafficking of molecules and cellular elements into the central nervous system (cns). horseradish peroxidase (hrp) exclusion is used as a measure of bbb integrity. the bbb is altered and becomes permeable during the course of experimental allergic encephalomyelitis (eae). heterotopic brain transplantation into the anterior eye chamber is a technique for studying genetic influences and the role of individual cell types on the develop ... | 1992 | 1371310 |
| reciprocal connections between the medial preoptic area and the midbrain periaqueductal gray in rat: a wga-hrp and pha-l study. | the midbrain periaqueductal gray (pag) participates in diverse functions such as analgesia, autonomic regulation, sexual behavior, and defense/escape responses. anatomical studies of the circuits involved in such functions have largely focused on the connections of pag with the medulla. projections to pag from forebrain structures are extensive, but their organization has received little attention. previous anatomic studies indicate that the medial preoptic area (mpo), involved in a variety of p ... | 1992 | 1371779 |
| interconnections between the primate cerebellum and midbrain near-response regions. | the goal of this study was to determine the pattern of the connections between the midbrain and cerebellum that may play a role in the modulation of the near-response in the macaque. injection of the retrograde tracer wheat germ agglutinin conjugated horseradish peroxidase (wga-hrp) into the physiologically identified midbrain near-response region, which includes the supraoculomotor area, labelled cells throughout the deep cerebellar nuclei. however, labelled cells were particularly concentrated ... | 1992 | 1371782 |
| anticonvulsant role of nigrotectal projection in the maximal electroshock model of epilepsy--ii. pathways from substantia nigra pars lateralis and adjacent peripeduncular area to the dorsal midbrain. | lesion evidence suggests that the superior colliculus is essential for mediating the anticonvulsant properties of nigral suppression in the electroshock model of epilepsy. however, our companion paper [redgrave et al. (1991) neuroscience 46, 379-390] established that the region of dorsal midbrain where bicuculline was most effective in suppressing tonic hindlimb extension did not correspond well with the known distribution of nigrotectal terminals. the purpose of the present anatomical study was ... | 1992 | 1371853 |
| the organisation of fibres within the rat basis pedunculi. | the organisation of corticofugal fibres within the basis pedunculi of rats was studied using wheat germ agglutinin-horseradish peroxidase as an orthograde tracer. following cortical injections, labelled fibres were distributed within the cerebral peduncle in an orderly way. fibres which originate from cells in the frontal cortex maintain a position in the ventromedial part of the basis pedunculi. fibres from the occipital and temporal cortex travel in the most dorsolateral part. somatosensory fi ... | 1992 | 1371860 |
| separate neuronal populations of the rat globus pallidus projecting to the subthalamic nucleus, auditory cortex and pedunculopontine tegmental area. | the topographic arrangement of globus pallidus neurons sending axons to the subthalamic nucleus, auditory cortex and pedunculopontine tegmental nucleus was studied in the rat using retrograde fluorescent tracers. neurons projecting to the subthalamic nucleus were localized in the rostral part of the globus pallidus, while neurons projecting to the auditory cortex and to the pedunculopontine tegmental nucleus were located in the caudal part. the two populations of pallidocortical and pallidotegme ... | 1992 | 1372116 |
| choline acetyltransferase-immunoreactive neurons in the rat entopeduncular nucleus. | using a monoclonal antibody against choline acetyltransferase, neurons of the rat entopenduncular nucleus were found to express choline acetyltransferase immunoreactivity. these cholinergic cells were located mostly in the rostral portion of the entopeduncular nucleus with a marked decrease towards its caudal portion. to identify their target sites, a retrograde fiber tracing technique was combined with immunohistochemistry for choline acetyltransferase. after injection of wheatgerm agglutinin c ... | 1992 | 1372118 |
| development and characterization of human anti-hbs antibodies. | peripheral blood mononuclear cells (pbmc) from 13 healthy hepatitis b vaccines were transformed with the epstein-barr virus (ebv) and lymphoblastoid cell lines (lcl) producing antibodies to hepatitis b surface antigen (anti-hbs antibodies). seven lcl and two clones secreting human anti-hbs monoclonal antibody were generated and their antibodies purified. one clone was fused with a mouse myeloma and the antibody from a cloned anti-hbs secreting heterohybridoma purified. one of the 10 purified hum ... | 1992 | 1372611 |
| new screening system for simultaneous determination of two marker proteins by homogeneous enzyme immunoassay. | to save time and labour in mass screening, by detecting two marker proteins on one specimen using only one test. | 1992 | 1372918 |
| substance p innervation of the rat and cat thalamus. ii. cells of origin in the spinal cord. | evidence in the preceding paper suggests that fibers and terminals immunopositive for substance p (sp) in somatosensory thalamic nuclei are part of the spinothalamic tract (stt). in this paper, more direct evidence on this point is provided by immunocytochemistry for sp on the cervical spinal cord, alone or combined with the retrograde transport of colloidal gold-labeled wheat germ agglutinin conjugated to enzymatically inactive horseradish peroxidase (wgaapohrp-au). in cats and rats pretreated ... | 1992 | 1373160 |
| [a morphological study of pulmonary macrophages in bleomycin-injected murine models]. | pulmonary changes induced in a murine model by intraperitoneal injections of bleomycin were morphologically studied by light and electron microscopy. the number of pulmonary macrophages and the distribution of fibronectin in these cells were evaluated by acid phosphatase and affinity staining using anti-fibronectin horseradish peroxidase conjugates. the onset of acute inflammation occurred 4 days after intraperitoneal injection of bleomycin and reached its peak on the 14th day post-injection. th ... | 1992 | 1373783 |
| chemiluminescent universal probe for bacterial ribotyping. | we describe a novel procedure for direct covalent coupling of horseradish peroxidase to rrna and ribotyping by using enhanced chemiluminescence. compared with their 32p-end-labeled counterparts, chemiluminescent rrna probes are stable and easy to synthesize and provide results as good as or superior to those obtained with isotopic labeling. direct chemiluminescent labeling of escherichia coli rrna produces a sensitive, universal probe suitable for clinical laboratory use in the investigation of ... | 1992 | 1374074 |
| organization of inferior olivary projections to the flocculus and ventral paraflocculus of the rat cerebellum. | the climbing fiber projection to the rat flocculus and adjacent ventral paraflocculus was investigated by using phaseolus vulgaris-leucoagglutinin as an anterograde and horseradish peroxidase as a retrograde tracer. large injections of horseradish peroxidase in the flocculus and ventral paraflocculus indicated that the climbing fibers to this region are derived exclusively from any of the following contralateral olivary regions: the dorsal cap of kooy, the ventrolateral outgrowth, the caudal hal ... | 1992 | 1374083 |
| claustrum in the hedgehog (erinaceus europaeus) brain: cytoarchitecture and connections with cortical and subcortical structures. | the cytoarchitecture of the claustrum in the hedgehog (erinaceus europaeus) brain, the morphology of its neurons, and the efferent connections with cortical and subcortical structures were studied with the nissl and klüver-barrera, the golgi, and the horseradish peroxidase methods. it was found that the claustrum is a well developed nucleus in the hedgehog telencephalon and, as in other mammals, is divided into dorsal and ventral parts. in golgi-stained sections, spiny multipolar cells are the p ... | 1992 | 1374084 |
| dorsal root ganglion neurons projecting to the dorsal column nuclei of rats. | dorsal root ganglion (drg) neurons may give origin to ascending branches that terminate in the dorsal column nuclei (dcn); uncertainties still exist with regard to the proportion of these neurons in different drgs and to the type of these neurons. the percentage and size of neurons that project to the dcn were determined in a large number of drgs by means of the retrograde transport of colloidal gold-labeled wheat germ agglutinin conjugated to enzymatically inactive horseradish peroxidase (wgaap ... | 1992 | 1374085 |
| trigeminocerebellar projections to the posterior lobe in the cat, as studied by anterograde transport of wheat germ agglutinin-horseradish peroxidase. | cerebellar projections of the nucleus interpolaris and oralis of the spinal trigeminal nucleus were studied in the cat by anterograde transport of wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp). injections of wga-hrp into these nuclei labeled many mossy fiber terminals mainly ipsilaterally in the rostral folium of lobule ix (ixa or ixa + b), the simple lobule, the anterior part (sublobule a) of the paramedian lobule and the posterior part of crus ii. labeled terminals were ... | 1992 | 1374086 |
| organization of parabrachial nucleus efferents to the thalamus and amygdala in the golden hamster. | while gustation in the hamster has been extensively studied at the behavioral and physiological level, very little is known about the central anatomy of the taste system. the purpose of this study was to trace the connections of the parabrachial nucleus (pbn) in the golden syrian hamster (mesocricetus auratus) using wheat germ agglutinin-conjugated horseradish peroxidase. the pbn is the site of the second central synapse for the ascending gustatory system and receives taste afferents from the nu ... | 1992 | 1374087 |
| cortical connections of the inferior arcuate sulcus cortex in the macaque brain. | injections of the retrograde/anterograde tracers wheat germ agglutinin-horseradish peroxidase (wga-hrp) into the cortex along the banks of the inferior limb of the arcuate sulcus in the cortex of 4 macaque monkeys (macaca fascicularis) were used to investigate its cortico-cortical connections. all injections produced transported label within the sulcus principalis, the ventral lateral prefrontal cortex, the anterior cingulate sulcus and the dorsal insular cortex. the distribution of label within ... | 1992 | 1374284 |
| receptor-mediated uptake and processing of vitamin d-binding protein in human b-lymphoid cells. | vitamin d-binding protein (dbp), a member of a multigene family including alpha-fetoprotein (afp) and albumin, is a serum glycoprotein that reversibly binds and transports vitamin d and its metabolites to target cells. in this work, we demonstrate that normal and malignant human b-lymphocytes specifically bind and internalize dbp. radioiodinated dbp (125i-dbp) was used to follow the uptake of the protein by raji cells, a human pre-b-lymphoma cell line. time course studies of dbp uptake by these ... | 1992 | 1374401 |
| time-dependent neuroplasticity in mesostriatal projections after unilateral removal of vibrissae in the adult rat: compartment-specific effects on horseradish peroxidase transport and cell size. | in adult rats the mystacial vibrissae were clipped on one side of the snout, and the influence of this sensory deprivation on crossed and uncrossed striatal afferents from the substantia nigra, ventral tegmental area, and retrorubral area was examined with the horseradish peroxidase tract tracing technique. unilateral removal of vibrissae was found to affect crossed and uncrossed nigrostriatal projections in a time-dependent manner. one to three days after hemivibrissotomy an apparent neuronal a ... | 1992 | 1374540 |
| disruptions of muscle fiber plasma membranes. role in exercise-induced damage. | the authors have tested the hypothesis that plasma membrane disruptions are an early form of structural damage to the fibers of eccentrically exercised muscle. rat serum albumin (rsa) was used as a marker for muscle-fiber wounding in the rat tricep (medial head) exercised eccentrically by downhill running. in all muscles examined, strong staining with a horseradish peroxidase (hrp)-conjugated anti-rsa antibody was observed between fibers (intercellular staining) and also within certain fibers (i ... | 1992 | 1374591 |
| ultrastructural evidence for neurogenically mediated changes in blood vessels of the rat dura mater and tongue following antidromic trigeminal stimulation. | we investigated the effects of unilateral electrical trigeminal ganglion stimulation (0.1 or 1.0 ma, 5 hz, 5 ms, 5 min) on the morphology of blood vessels within the rat dura mater and tongue using light and transmission electron microscopy. stimulation at both intensities caused changes which were confined to the ipsilateral post-capillary venules except in the tongue where arterioles were affected as well. changes were more marked after 1.0 ma. dramatic increases in the numbers of endothelial ... | 1992 | 1374861 |
| morphology and electrophysiology of superior laryngeal nerve afferents and postsynaptic neurons in the medulla oblongata of the cat. | intra-axonal recordings were made from 24 afferent fibres of the superior laryngeal nerve in and around the nucleus tractus solitarius, in 26 pentobarbitone-anaesthetized cats. conduction velocity ranged from 15 to 38 m/s. four afferents were injected with horseradish peroxidase. they showed dense terminal arborization in the region of the ventral and ventrolateral subnuclei of the nucleus tractus solitarius, both rostral and caudal to the obex. six other intra-axonal recordings were thought to ... | 1992 | 1374862 |
| growth cones and structural variation of synaptic end-bulbs in the cochlear nucleus of the adult cat brain. | to explore the potential for structural variation and new growth at the synapse, we studied the morphological patterns of the end-bulbs of cochlear nerve axons in adult cats by using rapid golgi, reduced silver, and electron microscopic methods. horseradish peroxidase labeling of these endings in the anterior division of the antroventral cochlear nucleus was produced by anterograde transport following injection into the cochlea. three types of end-bulbs were distinguished, regardless of method: ... | 1992 | 1374935 |
| segregated thalamocortical pathways to inferior parietal and inferotemporal cortex in macaque monkey. | inferior parietal and inferotemporal cortex, which process different aspects of visual information through largely segregated pathways from the visual cortex, both receive thalamic afferents from the pulvinar complex. we examined the topography of pulvinar projections to these two cortical regions by placing multiple injections of different tracers (fluorescent dyes, horseradish peroxidase) in the inferotemporal and inferior parietal cortex of macaque monkeys. the patterns of label observed afte ... | 1992 | 1375095 |
| ocular dominance columns in area 17 of old world macaque and talapoin monkeys: complete reconstructions and quantitative analyses. | the effects of monocular deprivation on cytochrome-oxidase (co) expression were used to reveal ocular dominance columns in flatmounts of the striate cortex in macaque (macaca fascicularis) and talapoin (miopithecus talapoin) monkeys. this procedure allowed the first direct visualization of the complete pattern of ocular dominance bands in macaque monkeys, and less complete reconstructions in talapoin monkeys. in a second macaque monkey, the ocular dominance organization was revealed by injecting ... | 1992 | 1375096 |
| an explanation of the achromatic bands produced by peroxidase isozymes in polyacrylamide electrophoresis gels stained for malate dehydrogenase. | when plant tissue extracts are electrophoresed on polyacrylamide gels and the gels are stained for malate dehydrogenase by the standard nad-dependent dehydrogenase reaction, terminating in the formation of reduced nitroblue tetrazolium (nbt), achromatic bands, in addition to the expected chromatic bands, are observed. the achromatic bands are seen when the staining conditions favor a generalized background staining of the gel and have been shown, in a previous study, to be caused by peroxidase i ... | 2013 | 1375153 |
| the medullary relay from neck receptors to somatosensory thalamus in the rat: a neuroanatomical study. | experiments were performed on rats to determine the location of thalamic projecting neurones in the medulla which receive direct contacts from neck primary afferents. the medullary terminations of primary afferents from the cervical region were identified by silver staining their degenerating terminals, diffusely filling their axons with horseradish peroxidase (hrp), or reacting for transganglionically transported hrp applied to muscle or cutaneous nerves. neurones projecting to the ventrobasal ... | 1992 | 1375163 |
| serotonin-, substance p- and tyrosine hydroxylase-like immunoreactive neurons projecting from the midbrain periaqueductal gray to the nucleus tractus solitarii in the rat. | serotonin-, substance p- and tyrosine hydroxylase-like immunoreactive neurons in the midbrain periaqueductal gray (pag) were observed to send their axons to the nucleus tractus solitarii in the rat by the retrograde horseradish peroxidase tracing method combined with the immunocytochemical technique. these neurons were most frequently observed in the ventrolateral subnucleus and ventral portion of the medial subnucleus of the pag at the entire rostrocaudal levels. | 1992 | 1375351 |
| endocytosis is regulated by protein kinase a, but not protein kinase c in a secretory epithelial cell line. | endocytosis in the chloride secreting epithelial cell line t84 was monitored by uptake of the fluid-phase markers fitc-dextran and horseradish peroxidase (hrp). uptake of marker was inhibited by incubation of cells at 4 degrees c, consistent with an endocytic uptake. although activation of the camp-dependent second messenger pathway has been shown to stimulate exocytosis in this cell line, it caused a 63% reduction in endocytosis as measured by uptake of fluid-phase markers. in contrast, the pre ... | 1992 | 1375455 |
| the nigral projection to predorsal bundle cells in the superior colliculus of the rat. | predorsal bundle cells give rise to the major efferent pathway from the superior colliculus to the premotor centers of the brainstem and spinal cord responsible for initiating orienting movements. the activity of predorsal bundle cells is profoundly influenced by an inhibitory pathway from substantia nigra pars reticulata that uses gamma aminobutyric acid (gaba) as a neurotransmitter. the present study examines the morphological basis for this influence of substantia nigra on predorsal bundle ce ... | 1992 | 1375604 |
| the somatosensory thalamus of monkeys: cortical connections and a redefinition of nuclei in marmosets. | thalamic connections of three subdivisions of somatosensory cortex in marmosets were determined by placing wheatgerm agglutinin conjugated with horseradish peroxidase and fluorescent dyes as tracers into electrophysiologically identified sites in s-i (area 3b), s-ii, and the parietal ventral area, pv. the relation of the resulting patterns of transported label to the cytoarchitecture and cytochrome oxidase architecture of the thalamus lead to three major conclusions. 1) the region traditionally ... | 1992 | 1375605 |
| direct w-like geniculate projections to the cytochrome oxidase (co) blobs in primate visual cortex: axon morphology. | the primate lateral geniculate nucleus (lgn) is composed of large, medium, and small cells located, respectively, in magnocellular (m), parvocellular (p), and specialized layers (intercalated and s-layers in simians, koniocellular (k) layers in prosimians). several studies have examined the physiology and connections of m and p lgn cells and have concluded that they provide separate contributions to visual perception via separate pathways. less is known about the structure and contributions of t ... | 1992 | 1375606 |
| spinal neurons exhibiting a specific nociceptive response receive abundant substance p-containing synaptic contacts. | substance p has been implicated in nociceptive transmission in the spinal cord. however, evidence for a direct correlation between a specific nociceptive response in spinal dorsal horn neurons and substance p input is lacking. in this study, we combine intracellular recording from dorsal horn neurons in vivo, characterization of their nociceptive responses, intracellular labeling by injection of horseradish peroxidase, and immunocytochemical demonstration of substance p at the electron microscop ... | 1992 | 1375757 |
| permeability of the macula densa basement membrane area to high molecular weight molecules. | to investigate the permeability properties of the basement membrane beneath macula densa cells and between extraglomerular mesangial cells, thick ascending limbs with attached glomeruli were dissected from rabbit kidney and incubated in a ringers solution containing either horseradish peroxidase (hrp) (1 mg/ml; molecular weight approximately 40,000) or native or cationic ferritin (both at 5 mg/ml molecular weight approximately 450,000) for 5 and 20 min at room temperature. tubules were processed ... | 1992 | 1375768 |
| alterations in lens permeability during galactose cataract development in rat. | lens permeability has been shown to be compromised during galactose-induced cataract development in rats. recent studies have demonstrated permeability and diffusion pathways as well as endocytotic activity in normal lenses of different species using tracers of different molecular weights. we investigated the permeability and diffusion of tracers in normal rat lenses and in lenses during cataract development using three different molecular weight tracers, lanthanum nitrate (ln, mw 430), rutheniu ... | 1992 | 1375837 |
| projection neurons of the basolateral amygdala: a correlative golgi and retrograde tract tracing study. | this study analyzed the projection neurons of the anterior subdivision of the rat basolateral amygdaloid nucleus (bla) by correlating the morphology of golgi-stained neurons with the morphology of neurons that were retrogradely labeled by injections into the main terminal fields of bla. in each animal multiple injections of horseradish peroxidase (hrp) and wheat germ agglutinin-conjugated hrp were made into the prefrontal cortex and rostral striatum. these injections labeled approximately 85% of ... | 1992 | 1375860 |
| effects of self- and cross-reinnervation on the numbers of motoneurons regenerating to forearm muscles in young and adult rats. | the present study was carried out to compare the ability of motoneurons to regenerate to functionally appropriate and inappropriate muscles, following axotomy at different stages of postnatal development. five-, 10-, 21-day-old and adult rats of both sexes were used. in one group, the right median and radial nerves were cut and reunited. in a second group, the cut nerves were cross reunited and, in a third group the nerves were merely exposed. following survival periods of up to one year, the ex ... | 1992 | 1375861 |
| the antigenicity of the carbohydrate moiety of an insect glycoprotein, honey-bee (apis mellifera) venom phospholipase a2. the role of alpha 1,3-fucosylation of the asparagine-bound n-acetylglucosamine. | a rabbit polyclonal antiserum raised against honey-bee (apis mellifera) venom phospholipase a2 (pla2) contains antibodies that react exclusively with its glycosylated variants and cross-react with plant glycoproteins. the interaction of anti-(horseradish peroxidase) antiserum with pla2 suggests the existence of a carbohydrate determinant common to both glycoproteins. e.l.i.s.a. binding and inhibition experiments, employing glycoproteins and glycopeptides of plant and animal origin with known n-g ... | 1992 | 1376112 |
| substance p-, vasoactive intestinal polypeptide-, and cholecystokinin-like immunoreactive fiber projections from the superior colliculus to the dorsal lateral geniculate nucleus in the rat. | substance p (sp)-, vasoactive intestinal polypeptide (vip)-, and cholecystokinin (cck)-like immunoreactive (li) neurons were found in the superior colliculus (sc) of the rat, and examined to ascertain whether they sent projection fibers to the dorsal lateral geniculate nucleus (lgnd). immunocytochemical staining with antisera against sp, vip, and cck showed that many immunoreactive neuronal cell bodies were located in the superficial layers of the sc, especially in the stratum griseum superficia ... | 1992 | 1376278 |
| the bilateral bulbar projections of the primary olfactory neurons in the frog. | whether or not the frog olfactory neuroreceptor cells project bilaterally to the olfactory bulb is still a debated question. we therefore decided to ascertain whether bilateral projections of the primary olfactory input exist and if so to investigate their extent. reproducible extracellular bilateral bulbar potentials were recorded in the frog following electrical stimulation of dorsal or ventral olfactory nerve bundles. the general features of the contralateral evoked responses were very simila ... | 1992 | 1376279 |
| intravenous injection of evans blue labels magnocellular neuroendocrine cells of the rat supraoptic nucleus in situ and after dissociation. | previous work has demonstrated that intravenous injection of neuronal tracers, e.g. horseradish peroxidase or fast blue, can retrogradely label neurons in brain areas that project outside the blood-brain barrier, e.g. magnocellular neuroendocrine neurons of the hypothalamus. here we have shown that 24 h after intravenous injection of the fluorescent retrograde tracer evans blue, the same population of magnocellular neuroendocrine neurons is labeled in the paraventricular, supraoptic and accessor ... | 1992 | 1376451 |
| identification of spinal electromotoneurons in the ray raja clavata (rajidae). | retrograde transport of horseradish peroxidase injected ipsilaterally in several parts of the electric organ and light microscopy were employed to study motoneurons and electromotoneurons of the spinal cord in the weakly electric ray raja clavata (rajidae). the horseradish peroxidase-labelled electromotoneurons form a chain of cells located ventrolaterally in the gray matter of the spinal cord segments adjacent to the electric organ. the diameters of cells in the electromotor nucleus vary from 7 ... | 1992 | 1376452 |
| serotonin-, substance p- and glutamate/aspartate-like immunoreactivities in medullo-spinal pathways of rat and primate. | serotonergic neurons of the medulla oblongata have been proposed to play a role in the control of sensory, motor and autonomic cells in the spinal cord. many of these raphe neurons have been shown to contain the undecapeptide substance p as well as the tripeptide thyrotropin-releasing hormone, but evidence for the presence of an excitatory amino acid in these pathways has not yet been documented. in colchicine-treated rats, we have used a combination of retrograde tracing and tri-color immunohis ... | 1992 | 1376453 |
| corticotropin-releasing factor innervation of the locus coeruleus region: distribution of fibers and sources of input. | electrophysiologic studies support the hypothesis that corticotropin-releasing factor, the neurohormone that initiates adrenocorticotropin release during stress, also serves as a neurotransmitter in the pontine noradrenergic nucleus, the locus coeruleus. to elucidate the circuitry underlying proposed corticotropin-releasing factor neurotransmission in the locus coeruleus, the present study utilized immunohistochemical techniques to characterize corticotropin-releasing factor innervation of rat l ... | 1992 | 1376457 |
| afferent connections of the rat substantia nigra pars lateralis with special reference to peptide-containing neurons of the amygdalo-nigral pathway. | the afferent connections of the rat substantia nigra pars lateralis have been studied using the retrograde axonal transport of fluorescent latex microspheres. the most numerous groups of retrogradely labelled nerve cell bodies were observed bilaterally in the parabrachial complex and several hypothalamic nuclei, whereas the parietal neocortex, the fundus striati, the central nucleus of the amygdala and the bed nucleus of the stria terminalis were labelled on the injected side only. the neuronal ... | 1992 | 1376607 |
| permeability of rat atrial endocardium, epicardium, and myocardium to large molecules. stretch-dependent effects. | atrial distension, which stimulates atrial natriuretic peptide secretion by atrial myocytes, also stretches nonmuscle cells. in a noncontracting in vitro preparation of combined right and left atria we demonstrated by electron microscopy that, at 37 degrees c, transition from zero pressure to a physiological distending pressure of 5.1 mm hg rapidly rendered atrial endocardial endothelium permeable to the macromolecular probes horseradish peroxidase (hrp; m(r), approximately 40,000) and wheat ger ... | 1992 | 1376644 |
| a comparative hrp study of the neuronal supply to the inferior and superior nasal meatus in the cat. | neurons supplying the nasal mucosa in the cat were retrogradely labelled with horseradish peroxidase. sensory trigeminal neurons to the inferior and superior nasal meati are somatotopically organized, according to the ophthalmic or maxillary origin of the afferents studied. whatever their relative location, the cell bodies from nasal afferents were, on average, smaller than the overall cell population in the ganglion, in keeping with the high proportion of nasal receptors innervated by thin fibe ... | 1992 | 1376879 |
| fastigiofugal fibers encoding horizontal and vertical components of saccades as determined by microstimulation in monkeys. | to identify the routes by which oculomotor vermis signals control eye movements (saccadic signals), saccades evoked by microstimulation were studied in the region of the uncinate fasciculus (uf) and juxtarestiform body (jb) in the macaque monkey. anatomical pathways of axons from the fastigial oculomotor region (for) were studied by anterograde transport of wheatgerm agglutinin conjugated horseradish peroxidase (wga-hrp). the routes were identified by comparing maps of low threshold for evoking ... | 1992 | 1376883 |
| enkephalin-immunoreactive nociceptive neurons in the cat spinal cord. | in this combined electrophysiological-ultrastructural study in the cat spinal cord, we detected enkephalin-like immunoreactivity using internally radio-labelled monoclonal antibodies in functionally characterized neurons which had been filled intracellularly with horseradish peroxidase. of the 4 neurons included in this study, two were positive for enkephalin immunoreactivity; one was a nociceptive specific neuron in lamina i, the other a wide dynamic range neuron in lamina v. the other two cell ... | 1992 | 1377041 |
| organization of cutaneous primary afferent fibers projecting to the dorsal horn in the rat: wga-hrp versus b-hrp. | primary afferent projections from cutaneous afferents in the forelimb and hindlimb digits to the dorsal horn (dh) were examined using 4 tracers: (1) 25% free horseradish peroxidase (hrp), (2) 2.5% wheat-germ agglutinin conjugated to horseradish peroxidase (wga-hrp), (3) a mixture of 25% free hrp and 2.5% wga-hrp (wga-hrp/hrp) or (4) 0.1% hrp conjugated to cholera toxin (b-hrp). the tracer was injected intracutaneously into the digits. three to 4 days later, the rats were perfused transcardially, ... | 1992 | 1377077 |
| a second auditory area in the non-cortical telencephalon of a reptile. | injections of horseradish peroxidase (hrp) into a caudocentral portion of the non-cortical telencephalon of caiman known as the dorsolateral area (dorsal ventricular ridge) resulted in retrogradely labeled neurons throughout the entire extent of the ipsilateral nucleus reuniens. hrp-positive cells were most numerous in nucleus reuniens pars diffusa with only sparse labeling of neurons in nucleus reuniens pars centralis. the results of the present experiment, when compared with those of a prior s ... | 1992 | 1377079 |
| the tectorecipient zone in the inferior olivary nucleus in the rat. | tecto-olivary and olivocerebellar projections in the rat were investigated in order to identify the tectorecipient zone in the inferior olivary nucleus and to determine whether inferior olivary neurons projecting to the cerebellar tecto-olivo-recipient zones (lobule vii, crus ii, lobulus simplex, and paramedian lobule) originate in the different regions within the tectorecipient zone. an electrophysiological method and an axonal transport technique of wheat-germ agglutinin-conjugated horseradish ... | 1992 | 1377203 |
| comparative study of laser and scalpel nerve transections. | this investigation was designed to compare standard scalpel transections of the tibial branch of the rat sciatic nerve with those performed using either a milliwatt carbon dioxide (co2) or a potassium titanyl phosphate (ktp/532) laser. four transection groups consisted of nerves sectioned with (1) scalpel (control), (2) milliwatt co2 laser, (3) ktp/532 with microscope attachment, and (4) ktp/532 laser with 400-microns bare fiber. each laser was used with the same parameters: 10 watts, 0.4-mm spo ... | 1992 | 1377320 |
| an enzyme-linked immunosorbent assay for urokinase-type plasminogen activator (u-pa) and mutants and chimeras containing the serine protease domain of u-pa. | an enzyme-linked immunosorbent assay (elisa) for quantitation of natural and recombinant plasminogen activators containing the serine protease domain (b-chain) of urokinase-type plasminogen activator (u-pa) was developed, based on two murine monoclonal antibodies, ma-4d1e8 and ma-2l3, raised against u-pa and reacting with non-overlapping epitopes in the b-chain. ma-4d1e8 was coated on microtiter plates and bound antigen was quantitated with ma-2l3 conjugated with horseradish peroxidase. the intr ... | 1992 | 1377417 |
| the superior cervical ganglion: origin of sympathetic fibers in the facial and hypoglossal nerves in the cat. | location of superior cervical ganglion (scg) neurons, sending axons into the facial and hypoglossal nerves, was investigated in the cat by means of retrograde axonal transport of horseradish peroxidase (hrp). after wheat germ agglutinin conjugated hrp (wga-hrp) was injected into these nerves, many retrogradely labeled neurons were found widely in the ipsilateral scg, particularly around the caudal half of the scg. these neurons were round or oval in shape and 70-80% of these were medium in size. ... | 1992 | 1377588 |
| intracellular band 3 immunostaining in type a intercalated cells of rabbit kidney. | intercalated cells (ics) in the collecting duct and the connecting tubule (cnt) are involved in h+ secretion and hco3- reabsorption. h+ secretion is mediated by an h(+)-adenosinetriphosphatase in the apical plasma membrane, whereas a band 3-like cl(-)-hco3- exchanger in the basolateral membrane is responsible for hco3- reabsorption. recent studies have reported that a band 3-like protein is also present in mitochondria in rabbit ics. the purpose of this study was to establish the subcellular loc ... | 1992 | 1377872 |
| serotonergic, noradrenergic and galaninergic projections to the nucleus parafascicularis. | immunocytochemical staining for serotonin (5-ht), tyrosine hydroxylase (th) and galanin (gal) was combined with horseradish peroxidase (hrp) retrograde tract-tracing technique to analyze the localizations of 5-ht-, catecholamine (ca)- and gal-containing neurons in the brainstem which project to the nucleus parafascicularis (pf) in rats. it is demonstrated that most of the retrogradely hrp-labeled neurons (70%) in bilateral periaqueductal gray (pag) and raphe nuclei are positively immunostained b ... | 1992 | 1377957 |
| somatotopic termination of the spino-olivary fibers in the cat, studied with the wheat germ agglutinin-horseradish peroxidase technique. | terminal sites of the spino-olivary fibers (sofs) were examined by the anterograde transport of wheat germ agglutinin-horseradish peroxidase in the cat. the tracer was injected at various spinal cord levels from the first cervical to the caudal segments. the sofs derived from the c1-t1 segments terminated medially in the caudal half (levels ii-viii of brodal) of the medial accessory olive (mao), which projects to the a zone of the cerebellar cortex, whereas the sofs derived from the l6-s1 segmen ... | 1992 | 1378027 |
| the medial dorsal nucleus is one of the thalamic relays of the cerebellocerebral responses to the frontal association cortex in the monkey: horseradish peroxidase and fluorescent dye double staining study. | to reveal the thalamic relay nucleus of the cerebellocerebral responses in the frontal association cortex, simultaneous labeling of the cerebellothalamic (c-t) terminals and the thalamocortical (t-cx) neurons was performed in three monkeys. horseradish peroxidase (hrp) was injected into the deep cerebellar nuclei and small doses of hrp or fluorescent dye were injected into the prefrontal cortex. the distribution of anterogradely labeled c-t terminals and retrogradely labeled t-cx neurons was exa ... | 1992 | 1378349 |
| sources of presumptive glutamatergic/aspartatergic afferents to the mediodorsal nucleus of the thalamus in the rat. | the distribution of presumptive glutamatergic and/or aspartatergic neurons retrogradely labeled following injections of 3hd-aspartate into the mediodorsal nucleus of the thalamus (md) in the rat was compared to the distribution of neurons labeled by comparable injections of the nonspecific retrograde tracer wheat germ agglutinin conjugated horseradish peroxidase (wga-hrp). cells retrogradely labeled by wga-hrp were found in the prefrontal and agranular insular cortices; in forebrain structures s ... | 1992 | 1378457 |
| enhancement of tonic and phasic events of rapid eye movement sleep following bilateral ibotenic acid injections into centralis lateralis thalamic nucleus of cats. | the excitotoxin ibotenic acid (1.2-2.6 microliters of 50 micrograms/microliters) was injected bilaterally into the thalamic centralis lateralis nucleus of chronically implanted cats in order to study the effects of tonic excitation followed by destruction of perikarya on the sleep-waking cycle and its electrographic correlates. ibotenate injections were performed under mild ketamine anaesthesia. immediately afterwards, the animals showed behavioural arousal accompanied first by ocular nystagmifo ... | 1992 | 1378577 |
| distribution of efferent neurons projecting to the tectum and cerebellum in the rat prepositus hypoglossi nucleus. | distributions of efferent neurons projecting to the superior colliculus (sc), the pretectum, and the cerebellar cortex were studied in the rat prepositus hypoglossi nucleus (prh) by retrograde labeling with wheat germ agglutinin-horseradish peroxidase (wga-hrp). after injection of the tracer into the unilateral sc, small- and medium-sized neurons were labeled throughout the entire rostrocaudal extent of the prh, with the highest proportion in the middle part of the nucleus. after application of ... | 1992 | 1378823 |
| endothelium-derived nitric oxide synthase inhibition. effects on cerebral blood flow, pial artery diameter, and vascular morphology in rats. | we determined the effects of inhibiting the production of cerebral endothelium-derived nitric oxide on pial artery diameter, cortical blood flow, and vascular morphology. | 1992 | 1378981 |
| increased elisa sensitivity using a modified method for conjugating horseradish peroxidase to monoclonal antibodies. | we investigated the importance of monoclonal antibody (mca) purity and the input molar ratio of horseradish peroxidase (hrpo)/igg used for mca conjugation on various immunoenzymometric assay (iema) parameters. the sensitivity of iemas for human follicle stimulating hormone (hfsh), human chorionic gonadotropin (hcg) and the free alpha subunit of hcg (hcg alpha) could be increased up to 6-fold, whereas non-specific binding remained within tolerable limits (e less than 0.1), when mcas purified by h ... | 1992 | 1379279 |
| role of nonacidic endosomes in recycling of adh-sensitive water channel structures. | toad urinary bladder epithelial cells respond to the hormone adh by increasing the water permeability of their luminal membrane. this action is mediated by insertion into the apical membrane of specific water channels. in the absence of adh these channels appear to be present in tubular cytoplasmic vesicles as morphologically distinctive intramembrane structures called particle aggregates. adh induces these vesicles to fuse with the apical membrane, transferring their aggregate-water channels in ... | 1992 | 1379533 |
| cortical afferents to behaviorally defined regions of the inferior temporal and parahippocampal gyri as demonstrated by wga-hrp. | the inferior temporal gyrus in the monkey appears to be unique among the many extrastriate visual cortices in its importance for normal performance of delayed match-to-sample, a visual memory task. however, the anatomical pathway providing visual information to this portion of the temporal lobe remains unclear. in this study, wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp) was injected into the anterior inferior temporal gyrus and heavy projections were found to arise in cyt ... | 1992 | 1380012 |
| afferent projections to the mammillary complex of the rat, with special reference to those from surrounding hypothalamic regions. | to better understand the functional organization of the mammillary nuclei, we investigated the afferents to this nuclear complex in the rat with iontophoretically injected wheat germ agglutinin conjugated to horseradish peroxidase. particular attention was paid to tracing local hypothalamic afferents to these nuclei. injections into the medial mammillary nucleus (mmn) revealed strong projections from the subicular region, and weaker projections from the prefrontal cortex, medial septum, and the ... | 1992 | 1380015 |
| a dopaminergic projection to the rat mammillary nuclei demonstrated by retrograde transport of wheat germ agglutinin-horseradish peroxidase and tyrosine hydroxylase immunohistochemistry. | the presence and distribution of dopaminergic neurons and terminals in the hypothalamus of the rat were studied by tyrosine hydroxylase (th) immunohistochemistry. strongly labelled th-immunoreactive neurons were seen in the dorsomedial hypothalamic nucleus, periventricular region, zona incerta, arcuate nucleus, and supramammillary nucleus. a few th-positive neurons were also identified in the dorsal and ventral premammillary nucleus, as well as the lateral hypothalamic area. th-immunoreactive fi ... | 1992 | 1380016 |
| the segmental distribution of afferent fibers from the vaginal cervix and hypogastric nerve in rats. | injections of horseradish peroxidase-wheat germ agglutinin (hrp-wga) into the walls of the vagina and cervix (vaginocervical injections) of rats resulted in labeling of dorsal root ganglia (drg) cells located at t11-l4 and l6-s2. in a second group of animals, exposure of the hypogastric nerve to hrp-wga resulted in a similar bimodal distribution of labeled cells as compared to vaginocervical injections. in a third group, unilateral hypogastric nerve transection prior to injection of hrp-wga into ... | 1992 | 1380393 |
| presence of gaba-immunoreactive neurons within intracortical patches in area 18 of the cat. | in cat visual cortex, horizontal, intracortical connections spread laterally to link together specific columnar sites. when visualized by retrograde tracers, these intracortical connections appear as periodic, columnar patches of dense cellular labeling interspersed with areas of much less dense labeling. we looked for anatomical evidence for direct inhibition among the patchy, horizontal connections in area 18, by combining retrograde labeling using wheat germ agglutinin (wga) conjugated to hor ... | 1992 | 1380396 |
| afferent projections to the cholinergic pedunculopontine tegmental nucleus and adjacent midbrain extrapyramidal area in the albino rat. i. retrograde tracing studies. | the afferent connections of the pedunculopontine tegmental nucleus (ppt) and the adjacent midbrain extrapyramidal area (mea) were examined by retrograde tracing with wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp). major afferents to the ppt originate in the periaqueductal gray, central tegmental field, lateral hypothalamic area, dorsal raphe nucleus, superior colliculus, and pontine and medullary reticular fields. other putative inputs originate in the paraventricular and preo ... | 1992 | 1380518 |
| progressive morphological abnormalities observed in rat spinal motor neurons at extended intervals after axonal regeneration. | it is generally accepted that mammalian spinal motor neurons return to normal after axotomy if their regenerated axons successfully reinnervate appropriate peripheral targets. however, morphological abnormalities, recently observed in spinal motor neurons examined 1 year after nerve crush injury, raise the possibility that delayed perikaryal changes occur after regeneration is complete. in order to distinguish between chronic and progressive alterations in neurons with long-term regenerated axon ... | 1992 | 1380520 |
| transneuronal labeling of cochlear nucleus neurons by hrp-labeled auditory nerve fibers and olivocochlear branches in mice. | auditory nerve fibers were labeled by extracellular injections of horseradish peroxidase into the spiral ganglion in mice. the labeled fibers were traced in an anterograde direction through the auditory nerve into the cochlear nucleus. in almost half of the injections, the labeled endings of auditory nerve fibers contacted cochlear nucleus neurons that were also labeled with horseradish peroxidase and were presumably transneuronally labeled. only darkly labeled endings were associated with trans ... | 1992 | 1380524 |
| retrograde labelling of dorsal root ganglion cells in the rat: a quantitative and morphological comparison of fluoro-gold with horseradish peroxidase labelling. | we have compared retrograde labelling of rat primary sensory neurons using fluoro-gold (fg) and horseradish peroxide conjugated with wheat germ agglutinin (hrp-wga). fluoro-gold 2.5% after 48 h transit time and fg 5% after 24 and 48 h retrogradely labelled similar numbers of cell profiles as hrp-wga (p greater than 0.1% student's t-test). the calculated cell size distribution for the above fg groups were similar to those for the hrp-wga. however, fg 2.5% after a 24 h transit time labelled signif ... | 1992 | 1380681 |
| topographical organization of the projections from physiologically identified areas of the motor cortex to the striatum in the rat. | the present study was undertaken to determine in the rat the topography of the neostriatal projections originating from the motor cortex. for that purpose, anterograde tracers (phaseolus vulgaris leucoagglutinin: pha-l; wheat germ agglutinin conjugated to horseradish peroxidase: wga-hrp) were deposited in discrete cortical sites physiologically identified by microstimulation. five major motor areas were considered in this study: the rostral (rfl) and caudal (cfl) forelimb areas, the hindlimb (hl ... | 1992 | 1380687 |
| the hypothalamo-cerebellar projection in the rat: origin and transmitter. | hypothalamic neurons projecting to cerebellum were identified by retrograde tracing with wheat germ agglutinin-horseradish peroxidase (wga-hrp) in the rat. selective d-[3h]aspartate labelling was used to investigate whether any of these connections may use excitatory amino acids as transmitters. the wga-hrp experiments revealed that the hypothalamo-cerebellar fibers have their main origins in the lateral, dorsal and posterior hypothalamic areas, and the tubero-mammillary nucleus, while smaller n ... | 1992 | 1380793 |