Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| characterisation and pharmacological sensitivity of antigen arthritis induced by methylated bovine serum albumin in the rat. | the optimum conditions for the induction of antigen-induced arthritis in the rat have been studied. two immunisations with methylated bovine serum albumin (mbsa) (0.5 mg) in freund's complete adjuvant (fca) containing 0.375 mg mycobacterium tuberculosis followed by an intra-articular injection of mbsa (0.5 mg) led to the production of a chronic, erosive arthritis. the development of the arthritis was associated with the appearance of t lymphocytes and other inflammatory cells in the synovium. ma ... | 1992 | 1509982 |
| lack of correlation between colony morphology and lipooligosaccharide content in the mycobacterium tuberculosis complex. | rough and smooth colony variants of the mycobacterium tuberculosis complex were compared with respect to their composition in trehalose-containing glycolipid antigens in view of the results of a recent investigation suggesting that the chemical basis of rough and smooth colony morphology in mycobacteria may reside in the occurrence of lipooligosaccharides. a careful chemical characterization of the individual glycolipids of the selected strains allowed the identification of the major glycolipids ... | 1992 | 1512581 |
| tuberculosis and leprosy: attempts to identify t-cell antigens of potential value for vaccine design. | tuberculosis and leprosy are chronic bacterial infectious diseases which represent major health problems worldwide. it is generally accepted that, on the one hand, effective vaccination strategies are required for satisfactory control of these diseases and, on the other hand, that currently available vaccination measures are insufficient for this purpose. ideally, a subunit vaccine should be designed which is composed of one or a few protective antigens. in this brief treatise our approach towar ... | 1992 | 1514057 |
| health care workers, tuberculosis, and the human immunodeficiency virus epidemic. | 1992 | 1514102 | |
| [drug resistance of mycobacterium tuberculosis]. | drug resistance of mycobacterium tuberculosis was followed up within a period of 1986 to 1989. there was a tendency to stabilization of resistance to streptomycin (42 per cent), tubazid (45.2 per cent) and ethionamide (up. to 1.4 per cent). resistance to kanamycin slowly increased (by 3 to 6 per cent every year). resistance to rifampicin markedly increased (almost 15 times). resistance to ethambutol decreased (10 times). it was shown that the drug resistance could be lowered by using the drug co ... | 1992 | 1514859 |
| polymerase chain reaction for detection of mycobacterium tuberculosis. | rapid diagnosis of tuberculosis is essential, and therefore we use a polymerase chain reaction. in this report, we describe two cases of tuberculous lymphadenitis in childhood. although histopathological findings were not specific for tuberculosis in both cases, distinct positive bands were amplified. for dna diagnosis of tuberculosis, a lysis method of extracting chromosomal dna from lipid-rich cell walls of mycobacteria is of critical importance. we made use of a simple lysozyme-proteinase k t ... | 1992 | 1515757 |
| the pattern of mycobacterial antigen recognition in sera from mantoux-negative individuals is essentially unaffected by bacille calmette-guérin (bcg) vaccination in either south india or london. | paired sera were obtained before and 8 weeks after routine bcg vaccination from 20 ppd-s mantoux-negative individuals who were living adjacent to the chingleput bcg vaccine trial area in tamil nadu and from seven mantoux-negative school-children in london, uk. most subjects became mantoux-positive after vaccination. in elisa tests against soluble extracts of bcg or mycobacterium tuberculosis h37rv or against ppd-s, pre-vaccination antibody titres of south indian subjects were about twice those o ... | 1992 | 1516256 |
| multidrug resistant mycobacterium tuberculosis in patients with human immunodeficiency virus infection. | multidrug resistant mycobacterium tuberculosis (mdr-mtb) infection has not been recognized as a serious problem in patients with human immunodeficiency virus (hiv) infection. multidrug resistance (mdr) has appeared in our medical center in 24 out of 72 patients between january 1990 and may 1991 compared to 8 out of 132 patients within the period from 1982 to 1987 (relative risk 5.50 with 95 percent confidence interval 2.61 to 11.61). we describe 19 patients with mdr in mtb (isoniazid and at leas ... | 1992 | 1516405 |
| homeless individuals and drug-resistant tuberculosis in south texas. | drug-resistant tuberculosis was found in 21 percent of homeless individuals in new york city between 1982 and 1987. to see if this relationship existed in south texas, we evaluated all admissions to a texas health department facility with culture-proven tuberculosis. four hundred forty-three patients were admitted between september 1987 and october 1990. twenty-six, (5.9 percent) of these patients were identified as homeless. alcoholism, tobacco abuse, divorce, and unemployment were common demog ... | 1992 | 1516406 |
| dependency on interleukin-1 of primary human in vitro t cell responses to soluble antigens. | the role of interleukin (il)-1 in antigen-specific activation of naive human t cells has been examined. primary human t cell proliferative responses to the soluble antigen keyhole limpet hemocyanin (klh) were decreased by neutralizing antisera to il-1 alpha (25 +/- 7% standard error) and il-1 beta (56 +/- 6% standard error). inhibition by both antisera in a primary culture was usually additive. recombinant il-1 alpha and recombinant il-1 beta could both re-establish responses in cultures blocked ... | 1992 | 1516626 |
| tuberculosis in the west of ireland 1986-1990. | a review of mycobacteria isolated from clinical samples from the western health board area (whba) for the years 1986 to 1990 was performed to establish the pattern of mycobacterial infection. the incidence of microbiologically proven cases of tuberculosis (13.3/100,000) and of sputum smear positive cases (6.2/100,000) was determined and correlated with notification data. m. bovis accounts for 6.3% of cases of microbiologically confirmed tuberculosis in this area. resistance to one or more of the ... | 1992 | 1517059 |
| use of polymerase chain reaction for early identification of mycobacterium tuberculosis in positive cultures. | to develop a readily applicable polymerase chain reaction (pcr) based technique which would permit the identification of mycobacterium tuberculosis complex isolates from bactec phials at an earlier stage than currently available methods. | 1992 | 1517460 |
| antibodies against secreted and non-secreted antigens in mice after infection with live mycobacterium tuberculosis. | mice from four different inbred strains were infected with live mycobacterium tuberculosis and the immune response to m. tuberculosis was followed for 24 weeks, using western blotting. nearly all mice, irrespective of h-2 type, reacted with the 38-kda protein band. antibodies against this secreted 38-kda protein were the first to appear, 4 weeks after infection. thereafter the secreted 19-kda protein and non-secreted antigens, such as the 65-kda and 33-kda proteins, were recognized. the immune r ... | 1992 | 1519034 |
| oxygen tensions and mycobacterial infections. | 1992 | 1520772 | |
| impact of human immunodeficiency virus infection on the epidemiology, clinical features, management, and control of tuberculosis. | after years of decreasing prevalence and increasing hope that tuberculosis, like smallpox, could be eliminated, the disease has resurfaced as a major public health problem in the united states. particularly ominous are the appearance of multiple-drug-resistant strains and their impact on patients and health care workers who are infected with the human immunodeficiency virus, among whom mortality rates reach 80% 2-3 months postdiagnosis. to respond effectively to this new threat, it is critical t ... | 1992 | 1520806 |
| interleukin-6 (il-6) in adjuvant arthritis of rats and its pharmacological modulation. | the levels of serum interleukin-6 (il-6) were measured during the course of adjuvant arthritis (faa) in male lewis rats. in the course of the disease serum il-6 levels increase with a clear correlation with morphologic disease signs. additionally, the faa-inducing antigen, heat-killed mycobacterium tuberculosis, was found to be a strong inducer of il-6 production by spleen cells in vitro. the effects of the anti-rheumatic drugs indometacin, dexamethasone, cyclophosphamide and cyclosporin a (csa) ... | 1992 | 1521924 |
| multidrug-resistant nosocomial tb: newest facet of the hiv epidemic. | 1992 | 1522165 | |
| relationships between titers of antibodies immunoreacting against glycolipid antigens from mycobacterium leprae and m. tuberculosis, the mitsuda and mantoux reactions, and bacteriological loads: implications in the pathogenesis, epidemiology and serodiagnosis of leprosy and tuberculosis. | analysis of cell-mediated immunity [(cmi) as judged from the mantoux, fernandez, and mitsuda reactions and the presence of granulomas in biopsy material] against humoral immunity (measurements of anti-pgl-i, pgl-tb1, and sl-iv igg and igm antibody titers by elisa) were performed in selected human populations. the investigations yielded data indicating that humoral (b-cell) responses preceded protective cmi in both tuberculosis and leprosy. the b-cell responses were unrelated to (unfavorable) cel ... | 1992 | 1522363 |
| [the bone marrow in human hiv infection. a bioptic study of 125 cases]. | bone marrow biopsies from 125 patients at different stages of hiv infection were examined and the histopathological changes are described. indications for biopsy included peripheral blood abnormalities, search for opportunistic pathogens, a suspected lymphoma or evaluation of its progression. common histopathological features, suggestive of hiv infection but non-pathognomonic, were: severe hypercellularity (43.2%), myelodysplasia (74.4%), plasmocytosis (86.4%), and lymphocytic (36.8%) and histio ... | 1992 | 1524353 |
| comparative ability of human monocytes and macrophages to control the intracellular growth of mycobacterium avium and mycobacterium tuberculosis: effect of interferon-gamma and indomethacin. | intracellular growth of mycobacterium avium and m. tuberculosis h37rv was compared both in human peripheral blood monocytes and in cultured macrophages. the cells were treated with 300 u of human recombinant interferon-gamma (ifn gamma) either 48 h prior to phagocytosis or after infection. in some cases, indomethacin (ind, a potent inhibitor of prostaglandin-e2 synthesis), was added immediately after infection of macrophages. ifn gamma pretreatment of monocytes resulted in about 50% lesser uptak ... | 1992 | 1524839 |
| the resurgence of tuberculosis. | 1992 | 1524865 | |
| detection of mycobacterium tuberculosis antigen in synovial fluid of patients with rheumatoid arthritis. | by use of antimycobacterial saline extract antibodies, mycobacterium tuberculosis (mt) antigens have been detected in synovial fluid (sf) of patients with rheumatoid arthritis (ra) by a highly specific and sensitive double-antibody sandwich enzyme-linked immunosorbent assay (elisa). absorbance for 15 gout, 14 osteoarthritis (oa) patients, 12 patients with spondylarthropathies (sa) and eight patients with other inflammatory disorders (oid) ranged from 0.001 to 0.025 with the mean values of 0.0086 ... | 1992 | 1525627 |
| molecular cloning and sequencing of the gene for mycocerosic acid synthase, a novel fatty acid elongating multifunctional enzyme, from mycobacterium tuberculosis var. bovis bacillus calmette-guerin. | mycocerosyl lipids are found uniquely in the cell walls of pathogenic mycobacteria. mycocerosic acid synthase (mas) is a multifunctional protein which catalyzes elongation of n-fatty acyl-coa with methylamalonyl-coa as the elongating agent (rainwater, d. l., and kolattukudy, p. e. (1985) j. biol. chem. 260, 616-623). to understand how the various domains that catalyze the reactions involved in chain elongation are organized, mas gene from mycobacterium tuberculosis bovis bcg was cloned. a lambda ... | 1992 | 1527058 |
| the 38-kda protein of mycobacterium tuberculosis: a review. | this review illustrates that the 38-kda protein is one of the most important antigens of mycobacterium tuberculosis. it is actively secreted but partly attached to the surface of the mycobacterial cell by a lipid tail that may also be responsible for binding of carbohydrate to the protein. it is a major constituent of m. tuberculosis culture fluid after growth on the synthetic sauton medium and occurs in bacille calmette-guérin in far lower concentrations. the protein induces b and t cell respon ... | 1992 | 1527425 |
| tuberculous mastoiditis. | the prevalence of mycobacterium tuberculosis is increasing due in part to the rising incidence of immunocompromised hosts. although mycobacterium tuberculosis is a well-documented pathogen in otitis media and mastoiditis, its extremely low incidence often precludes consideration when treating these infections. this is especially true when concomitant pulmonary symptoms are absent. we describe two patients who initially presented after prolonged unsuccessful medical and surgical therapy for chron ... | 1992 | 1527837 |
| [epidemiological hazardousness of patients with renal tuberculosis]. | a total of 408 nephrotuberculosis patients detected in a large industrial city for the period of 1975-1989 and 645 subjects from their family and residence surroundings were subjected to a current and retrospective epidemiological examination to evaluate the epidemiological danger presented by the patients sick with active tuberculosis of the urinary organs. washings collected from the various objects in 100 apartments of these patients were studied for m. tuberculosis in the genitourinary and p ... | 1992 | 1528823 |
| [characteristics of bacterial excretion in pulmonary tuberculosis patients residents of the yakut region]. | the present state of bacilli excretion in the yakut area and its certain social aspects are characterized, the detection rate of m. tuberculosis in pulmonary tuberculosis and the sex-age structure are subjected to analysis. data on the primary and secondary drug resistance, type appertainance of the causative agent and the result of a disinfectant work in the infection foci have been studied in 511 patients having copious bacilli excretion. | 1992 | 1528835 |
| [quantitative and qualitative characteristics of bacterial excretion in socially maladapted patients with pulmonary tuberculosis and outcomes of the process]. | a clinico-bacteriologic examination comprised 103 patients with destructive forms of pulmonary tuberculosis who excreted the bacterial and l-forms of m. tuberculosis. patients were divided into two groups depending on their social adaptation. group i included 66 patients with asocial behaviour, group 11-37 patients whose way of life was normal. patients of group i exhibited copious excretion of m. tuberculosis and l-forms with a high degree drug resistance and multiresistance of m. tuberculosis ... | 1992 | 1528836 |
| culture-positive tuberculosis in western australia. | notifications of 485 patients with culture-positive tuberculosis (tb) in western australia from 1980 to 1989 inclusive have been analysed. in 478 (98.6%) the disease was caused by mycobacterium tuberculosis hominis and in seven (1.4%) m. bovis. most (78.5%) of the disease was pulmonary with 4.3% pleural and 17.2% extrapulmonary. the annual incidence decreased from 4.6 per 100,000 in 1980 to 2.5 in 1985 steadying thereafter around 3.3. the aborigines had over four times the average incidence of t ... | 1992 | 1530530 |
| multi-drug resistant tuberculosis in migrants. | 1992 | 1530543 | |
| peritoneal tuberculosis: laparoscopic patterns and its diagnostic accuracy. | we report laparoscopic findings in 38 proven cases of peritoneal tuberculosis. the laparoscopic appearances can be classified into three types: thickened peritoneum with miliary yellowish white tubercles with or without adhesions (n = 25), only thickened peritoneum with or without adhesions (n = 8), and fibroadhesive pattern (n = 5). biopsies were avoided from fibroadhesive lesions due to risk of complications. visual diagnosis was accurate in 95% of patients. in comparison, in 27 (82%) of 33 pa ... | 1992 | 1530803 |
| a protective role of gamma/delta t cells in primary infection with listeria monocytogenes in mice. | we have previously reported that t cells bearing t cell receptors (tcrs) of gamma/delta type appear at a relatively early stage of primary infection with listeria monocytogenes in mice. to characterize the early-appearing gamma/delta t cells during listeriosis, we analyzed the specificity and cytokine production of the gamma/delta t cells in the peritoneal cavity in mice inoculated intraperitoneally with a sublethal dose of l. monocytogenes. the early-appearing gamma/delta t cells, most of which ... | 1992 | 1530961 |
| gamma interferon and interleukin 2, but not interleukin 4, are detectable in gamma/delta t-cell cultures after activation with bacteria. | mycobacterium tuberculosis- or group a streptococcus-activated gamma/delta t cells from normal healthy individuals were negatively sorted and restimulated in vitro from 48 h. significant amounts of gamma interferon were detected after restimulation with m. tuberculosis, group a streptococci, or listeria monocytogenes. in contrast, interleukin 4 was undetectable in the culture supernatants. our findings provide indirect evidence for the involvement of gamma/delta t cells in immunity against tuber ... | 1992 | 1531813 |
| modulation of expression of delayed hypersensitivity by mycobacterial antigen 85 fibronectin-binding proteins. | although demonstration of delayed hypersensitivity to purified protein derivative of tuberculin (ppd) is an important element in the diagnosis of infection with mycobacterium tuberculosis, many patients with tuberculosis are anergic. several possible mechanisms for this specific lack of response have been described. we have now uncovered an additional one. t-cell fibronectin (fn), a lymphokine secreted by activated t cells, is closely associated with the initiation of delayed hypersensitivity re ... | 1992 | 1534074 |
| [a case of pulmonary tuberculosis associated with severe skin eruption, prominent eosinophilia, and liver dysfunction induced by streptomycin]. | a 42-year-old woman was admitted because of cough, sputum, and fever. a chest roentgenogram revealed a nodular density in the left upper lung field with satellite lesions compatible with tuberculoma. mycobacterium tuberculosis was detected from sputum. five weeks after starting the treatment with 0.4 g/day of isoniazid, 0.45 g/day of rifampicin, and 0.75 g/day of streptomycin, she showed itching erythema in the trunk. the white blood cell count was 4,500/mm3 with 14% eosinophils, and serum trans ... | 1992 | 1534589 |
| supernatants derived from cd8+ lymphocytes activated by mycobacterial fractions inhibit cytokine production. the role of interleukin-6. | our study examined the effects of supernatants derived from cd8+ lymphocytes treated with high molecular weight components of mycobacterium tuberculosis on cytokine production. such suppressor but not control supernatants increased the production of il-4 and il-6 whilst suppressing il-1 beta, tnf-alpha, il-2 and ifn-gamma production by monocytes and lymphocytes. the effects on cytokine production were time dependent being observed as early as 4 hours with peak activity observed at 24 hours. the ... | 1992 | 1535780 |
| gamma delta t lymphocytes in human tuberculosis. | the manifestations of tuberculous infection reflect the immune response to infection. most healthy tuberculin reactors develop protective immunity; tuberculous pleuritis reflects a resistant response manifest by mild disease, whereas advanced pulmonary and miliary tuberculosis reflect ineffective immunity. the role of gamma delta t cells was assessed in tuberculous infection by evaluating expansion of these cells from blood mononuclear cells after stimulation with mycobacterium tuberculosis. aft ... | 1992 | 1538155 |
| human leukocyte antigen, tuberculosis, and mycobacterium tuberculosis-specific antibody. | 1992 | 1538172 | |
| morphometric analysis of mycobacterium tuberculosis infection in mice suggests a genetic influence on the generation of the granulomatous inflammatory response. | there is evidence in natural human disease and experimental infection in mice that host genetic factors influence susceptibility to infection with mycobacterium tuberculosis and the progress of the disease. in mouse models, both h-2 and non-h-2 genes have been implicated. in this study, four inbred strains of mice (balb/b, balb/k, b10, b10.br), selected for combinations of two different h-2 haplotypes on two different non-h-2 backgrounds, were inoculated with m. tuberculosis, strain h37rv, by in ... | 1992 | 1538277 |
| arthritis associated with adjuvant mycobacterial treatment for carcinoma of the bladder. | 1992 | 1538864 | |
| structural basis of capacity of lipoarabinomannan to induce secretion of tumor necrosis factor. | the results of this study show that lipoarabinomannans (lam) isolated from a virulent strain and from an avirulent strain of mycobacterium tuberculosis, which have recently been shown to differ markedly in terms of the structures of their nonreducing termini, also differ markedly in the capacity to induce the secretion of tumor necrosis factor from murine macrophages. it was found that lam from the avirulent h37ra strain was 100-fold more potent at inducing tumor necrosis factor secretion than l ... | 1992 | 1541542 |
| decline and fall of the tubercle bacillus: the newcastle story 1882-1988. | 1992 | 1543390 | |
| pulmonary tuberculosis in hiv-infected patients with normal chest radiographs. | subjects: three hiv-infected patients with active pulmonary non-disseminated tuberculosis and normal chest radiograph at clinical presentation and during follow-up are reported. patients had cough and fever but no other specific symptoms. löwenstein cultures of specimens from bronchoalveolar lavage in two cases and induced sputum in one yielded mycobacterium tuberculosis. conclusions: the diagnosis of tuberculosis in hiv-infected patients depends greatly on clinical suspicion by the physician, b ... | 1992 | 1543571 |
| tuberculosis in hiv seropositive individuals--a retrospective analysis. | a retrospective analysis of all culture-positive cases of mycobacterium tuberculosis infection in hiv positive individuals, over a 5 year period, revealed 18 cases, drawn from a population of approximately 1500. the prevalence of culture proven m. tuberculosis over the 5 year period was therefore 1.2% and was strongly associated with either a concomitant, or a subsequent, aids diagnosis. sixty-one per cent had pulmonary tuberculosis, 17% had both extra-pulmonary and pulmonary infection and 22% h ... | 1992 | 1543766 |
| mycobacterial protein antigens: a compilation. | in response to recommendations from the steering committees responsible for co-ordination of world health organization programmes for research on the immunology of leprosy (immlep) and tuberculosis (immtub), a list was prepared summarizing the properties of mycobacterial proteins currently under investigation with respect to their immunological activities. after consultation with more than 40 laboratories world-wide this list was extended to form the compilation shown below and is intended to pr ... | 1992 | 1545700 |
| tuberculosis rebounds while funding lags. | 1992 | 1546306 | |
| beta-lactamase inhibitors and the inducibility of the beta-lactamase of mycobacterium tuberculosis. | ten clinical isolates and the type strain (h37rv) of mycobacterium tuberculosis were shown to produce an intracellular beta-lactamase. crude enzyme preparations were extracted from acetone cell powders by grinding with zirconium beads in 0.133 m glycine with 1.0% triton x-100. the enzymes had identical patterns on isoelectric focusing, with two major bands at isoelectric points of 4.9 and 5.1. the beta-lactamase was highly susceptible to the new beta-lactamase inhibitor brl 42715, with an i50 of ... | 1992 | 1546847 |
| mycobacterium gordonae genitourinary disease. | mycobacterium gordonae is frequently isolated from urine, but m gordonae genitourinary disease is rare; the majority of the isolates are commensals. we describe a 40 year old housewife who presented with loin pain, dysuria and frequency. urine contained excessive pus cells, was sterile on culture and she did not respond to broad spectrum antibiotics. there was repeated isolation of m gordonae from the urine and she responded to a standard antituberculosis regimen. she was irregular and non-compl ... | 1992 | 1548012 |
| heterogeneity of the repertoire of t cells of tuberculosis patients and healthy contacts to mycobacterium tuberculosis antigens separated by high-resolution techniques. | in this report, we describe studies to examine the repertoire of freshly isolated human t lymphocytes to 400 distinct antigen fractions of mycobacterium tuberculosis separated by a novel method involving two-dimensional gel electrophoresis. separated antigens were probed with t cells from tuberculosis patients and purified protein derivative (ppd)-positive (ppd+) and ppd-negative (ppd-) contacts as well as normal healthy donors. t cells from all donors tested responded to separated antigens. sti ... | 1992 | 1548098 |
| failure of the 12 months course of antituberculous chemoprophylaxis in hiv infection. | 1992 | 1548412 | |
| killing of virulent mycobacterium tuberculosis by reactive nitrogen intermediates produced by activated murine macrophages. | tuberculosis remains one of the major infectious causes of morbidity and mortality in the world, yet the mechanisms by which macrophages defend against mycobacterium tuberculosis have remained obscure. results from this study show that murine macrophages, activated by interferon gamma, and lipopolysaccharide or tumor necrosis factor alpha, both growth inhibit and kill m. tuberculosis. this antimycobacterial effect, demonstrable both in murine macrophage cell lines and in peritoneal macrophages o ... | 1992 | 1552282 |
| [rapid diagnosis of mycobacteria, especially mycobacterium tuberculosis and mycobacterium avium complex, using bactec 460 tb system]. | fourty-five sputum specimens collected at the national sanatorium hiroshima hospital were subjected to cultivation using either bactec 460 tb system (bactec method; becton dickinson co., towson, md., u.s.a.) or 3% ogawa egg medium. test sputum was treated with four volumes of 4% naoh for approximately two minutes, after which 0.1 ml of the treated sputum was immediately inoculated onto 3% ogawa egg medium. after neutralizing the remaining pretreated sputum with 1n hcl, and diluting with 1/15 m p ... | 1992 | 1552698 |
| [rapid detection of mycobacteria in sputa using media supplemented with culture filtrate of gemella haemolysans]. | the effect of the culture filtrate gemella haemolysans in enhancing mycobacterial growth has been previously demonstrated. in the present studies, an attempt was made to confirm whether the addition of the filtrate into the medium would be an effective method to promote the rapid detection of mycobacteria in sputum specimens of patients. one-hundred and one sputum specimens pretreated with naoh were inoculated with various media (dubos, dubos-agar, 1% ogawa, kudo pd, and middlebrook 7h9) supplem ... | 1992 | 1552699 |
| on the track of "killer" tb. | 1992 | 1553538 | |
| delay in respiratory isolation of patients with pulmonary tuberculosis and human immunodeficiency virus infection. | 1992 | 1554143 | |
| reduced intracellular growth of mycobacteria in human macrophages cultivated at physiologic oxygen pressure. | the growth of mycobacteria in human macrophages was examined at an ambient concentration of oxygen (5% co2 and 95% air, corresponding to 20% o2 or 140 mm hg po2) and at a concentration corresponding to tissue levels (5% o2 and 5% co2 in nitrogen balance, corresponding to 36 mm hg po2). compared with the higher po2 level, macrophages cultivated at lower po2 level spread more widely and had an increased glycolytic and decreased oxidative metabolism. upon pma stimulation, they displayed a better pr ... | 1992 | 1554224 |
| comparison of a commercial dna probe test and three cultivation procedures for detection of mycobacterium paratuberculosis in bovine feces. | diagnosis of paratuberculosis using the idexx dna probe test and 3 methods for cultivation of mycobacterium paratuberculosis from fecal specimens were compared. twenty-one of 170 fecal specimens were dna probe test positive, whereas 35 specimens were positive by 1 or more of the cultivation methods evaluated. four specimens were dna probe test positive but were negative by fecal culture. the probe test detected m. paratuberculosis dna in 62.9% of the specimens positive by a sedimentation culture ... | 1992 | 1554765 |
| lipoarabinomannan. multiglycosylated form of the mycobacterial mannosylphosphatidylinositols. | the lipopolysaccharides of mycobacteria, lipoarabinomannan (lam) and lipomannan (lm), of key importance in host-pathogen interaction, were recently shown to contain a phosphatidylinositol "anchoring domain." we now have established that lam and lm are based on the phosphatidylinositol mannosides, the characteristic glycophospholipids of mycobacteria. digestion of the arabinose-free lm with an endo-alpha 1----6-mannosidase yielded evidence for the presence of the 1-(sn-glycerol-3-phospho)-d-myo-i ... | 1992 | 1556131 |
| lipoarabinomannan of mycobacterium tuberculosis. capping with mannosyl residues in some strains. | previously we had demonstrated that the termini of the arabinan component of mycobacterial cell wall arabinogalactan, the site of mycolic acid location, consists mostly of clusters of a pentaarabinofuranoside, [beta-d-araf-(1----2)-alpha-d-araf-(1----]2----(3 and 5)-alpha-d-araf. subsequently, the same arrangement was shown to dominate the non-reducing ends of lipoarabinomannan (lam), a key component in the interaction of mycobacteria with host cell. accordingly, we had proposed that mycobacteri ... | 1992 | 1556132 |
| antimycobacterial activity of a series of pyrazinoic acid esters. | a series of pyrazinoic acid esters has been prepared and evaluated for in vitro antimycobacterial activity. several of the pyrazinoate esters have substantially better activity than the first-line antituberculous agent pyrazinamide against susceptible isolates of mycobacterium turberculosis as well as activity against pyrazinamide-resistant isolates. the minimal inhibitory concentrations (mics) were lower for each organism and at each ph than the mics for pyrazinamide. the esters have activity a ... | 1992 | 1560435 |
| [paragonimiasis and pulmonary tuberculosis]. | paragonimiasis (infestation by the paragonimus species) in spain is a very infrequent entity within the group of imported infectious diseases. a native, resident of equatorial guinea who was affected by pulmonary and probably extrapulmonary paragonimiasis together with active pulmonary tuberculosis is described. this association is relatively common and may complicate the diagnostic process. the identification of the parasite was established from samples of pulmonary secretion obtained by natura ... | 1992 | 1560702 |
| molecular cloning and nucleotide sequence analysis of the gene encoding the immunoreactive brucella abortus hsp60 protein, ba60k. | a recombinant 60 kda brucella abortus protein expressed in escherichia coli was recognized in immunoblots by sera from mice experimentally infected with b. abortus and a dog experimentally infected with b. canis. sera from humans and dogs with naturally acquired brucellosis also recognized this protein, which was designated ba60k. the gene encoding ba60k was localized within an 18 kb b. abortus genomic fragment and its direction of transcription determined by subcloning and maxicell analysis of ... | 1992 | 1560753 |
| tuberculosis of the 1990s: the quiet public health threat. | therapeutic advances over the last four decades have led to a significant reduction of new cases of mycobacterium tuberculosis (tb) and allowed nearly 600 sanatoria to close in america. the number of active cases of tb in 1984 decreased to nearly 25 percent of those reported in 1953. it appeared that the u.s. was headed toward a utopian state of total control or even elimination of tb. however, geopolitical, socioeconomical, and medical factors contributed to an increased tb incidence altering t ... | 1992 | 1560986 |
| [mycobacterium tuberculosis. from the gene to the diagnosis]. | pulmonary tuberculosis still in on the list of the world major health problems. tuberculosis has not been eradicated yet, from developing countries. furthermore, its incidence is increasing in the industrialized world, due to the human immunodeficiency virus (hiv) epidemic. in this regard, atypical clinical presentation of tuberculosis in individuals who have a deficient immune system, such as those at risk of tuberculosis because of hiv infection, makes the diagnostic process more difficult. tu ... | 1992 | 1561482 |
| primary drug resistance in mycobacterium tuberculosis in the greater cape town area. | 1992 | 1561570 | |
| mycobacterial disease in hiv-infected persons. | 1992 | 1561929 | |
| immunization of mice with the antigen a60 of mycobacterium bovis bcg. | antigen 60 (a60) is a thermostable component of the cytoplasm of mycobacterium tuberculosis and bcg which can be fractionated into at least 15 protein bands when analysed by western blot. normal b6d2 mice were immunized subcutaneously with 20 micrograms of the a60 protein suspended in freund's incomplete adjuvant (fia) or in saline. three weeks later the mice received a second dose of vaccine followed 2 weeks later by an aerogenic challenge with approximately 10(3) cfu of m. tuberculosis erdman. ... | 1992 | 1563099 |
| detection of mycobacterial antigens in sputum by an enzyme immunoassay. | an enzyme immunoassay (eia) for the detection of mycobacterial antigens in sputum was evaluated. the system utilises commercially available anti-bcg immunoglobulin. bcg protein standard was used as positive control. thirty-nine patients with culture-proven pulmonary tuberculosis were tested. the eia was positive in 24 of 29 patients with positive smears and cultures, giving a sensitivity of 86.2%. it was also positive in six of ten patients with smear-negative culture-positive disease, resulting ... | 1992 | 1563387 |
| characterization of the major membrane protein of virulent mycobacterium tuberculosis. | a protein with a molecular mass of 19 kda was isolated and purified from enriched membrane fractions of the virulent erdman strain of mycobacterium tuberculosis. the protein is different from another 19-kda protein, a lipoprotein, that was recently described (d. b. young and t. r. garbe, res. microbiol. 142:55-65, 1991). the sequencing strategy applied to this major membrane protein employed four different endoproteinases and resulted in sufficient overlapping peptide sequences for assignment of ... | 1992 | 1563797 |
| lupus vulgaris. clinical, histopathologic, and bacteriologic study of 10 cases. | lupus vulgaris (lv) represents the most common form of cutaneous tuberculosis in europe. however, recent reports from european countries are few and usually limited to isolated cases. | 1992 | 1564144 |
| reactivation of tuberculous lymphadenitis during pregnancy. | three patients presenting with tuberculous lymphadenitis during pregnancy are described. by suppressing cell-mediated immunity, pregnancy predisposes the mother to infections of the type in which cellular immunity is important in defence. in this setting, self-healed primary pulmonary or extra-pulmonary tuberculosis may reactivate. | 1992 | 1569309 |
| a fastidious strain of mycobacterium tuberculosis in a patient with aids. | 1992 | 1569993 | |
| metabolic studies on connective tissue collagens in bone and tendon of adjuvant arthritic rat. | the metabolic alterations in the matrix of connective tissue collagen were investigated in bone and tendon of rats with adjuvant-induced arthritis. adjuvant arthritis was induced in rats with the immunization of freund's adjuvant containing heat-killed mycobacterium tuberculosis. the changes in the metabolism of collagen were studied using radioactive isotopic measurements with (3h)-proline. tissue specimens were fractionated individually into soluble and insoluble collagens. the synthesis of co ... | 1992 | 1571843 |
| serodiagnosis and follow up of patients with pulmonary tuberculosis by enzyme-linked immunosorbent assay. | a new elisa assay based on antigen a60 from m. bovis bcg was used to quantitate specific anti-mycobacterial antibodies in 250 sera from 133 subjects: 90 tuberculosis cases and 43 controls. controls were all negative, suggesting the specificity of this assay. in subjects with secondary pulmonary tuberculosis, a correlation was observed between the anti-mycobacterial antibody titer and culture positivity. in fact, positive elisa assays were found in 88.8% of patients with positive cultures for m. ... | 1992 | 1572435 |
| comparison of mb-check, bactec, and egg-based media for recovery of mycobacteria. | the rate of recovery and time to the detection of mycobacteria from clinical specimens were measured for biphasic (mb-check; nippon roche co., ltd., tokyo, japan) and radiometric (bactec; nippon becton dickinson co., ltd., tokyo, japan) liquid-based culture systems and egg-based media (3% ogawa and ogawa k). from the 245 sputum specimens processed, a total of 86 (35.1%) mycobacterial isolates were detected. of these, 81 (94.2%) and 80 (93.0%) isolates were detected with the mb-check and bactec s ... | 1992 | 1572974 |
| molecular cloning of a highly repeated dna element from mycobacterium tuberculosis and its use as an epidemiological tool. | in order to develop a technique for distinguishing between isolates of mycobacterium tuberculosis, we cloned two hypervariable dna fragments from ndeii-digested genomic dna. the cloned dna fragments of 3.8 and 4.7 kb were found to contain the same repetitive element, which was different from previously characterized repetitive elements. it is present in at least 30 copies per genome and is distributed among mycobacterial species other than those of the tuberculosis complex, including m. kansaii, ... | 1992 | 1572981 |
| mycobacteria and aids. | mycobacteria are acid-fast, slow-growing microorganisms which have gained attention due to increasing prevalence in aids patients. until the advent of aids, the only true pathogens of this group were mycobacterium tuberculosis and m. leprae and the remaining mycobacteria were considered to be saprophytes or opportunistic pathogens. infection with the mott (mycobacteria other than tuberculosis) bacilli was only seen in elderly or immunocompromised patients and was generally limited to caseating p ... | 1992 | 1573382 |
| a study of tubercular antigen and antibody in childhood tuberculosis. | a radioimmunoassay for the detection of tubercular (tb) antigen (ag) and antitubercular antibody (ab) was evaluated for the serodiagnosis of childhood tuberculosis. children with primary complex, progressive primary complex, miliary tuberculosis, and calcified lung lesions without clinical evidence of active tuberculosis were studied. significantly elevated levels of tb ag and tb ab isolated from the circulating immune complexes were obtained in primary, progressive primary, and miliary tubercul ... | 1992 | 1573688 |
| nosocomial transmission of tuberculosis in a hospital unit for hiv-infected patients. | to assess nosocomial transmission of tuberculosis (tb). | 1992 | 1573751 |
| impact of hiv infection on mycobacterial disease. | the current epidemic of human immunodeficiency virus (hiv) infection and acquired immunodeficiency syndrome has resulted in a worldwide resurgence of tuberculosis. in the united states, the number of new cases of tuberculosis has increased in the past five years, leading to a cumulative excess of about 15,000 cases. the clinical and epidemiologic picture of tuberculosis in aids patients differs drastically from the standard presentation of tuberculosis cases in hiv-negative patients. the diagnos ... | 1992 | 1575115 |
| tuberculosis and the surgeon. | with the resurgence of active tuberculosis in the united states, surgeons may be called upon to participate in the management of primary tuberculosis as well as major complications of the disease. to define the role of surgery in the diagnosis and treatment of tuberculosis in the 1990s, a 5-year retrospective review of 121 tuberculosis patients requiring invasive procedures in the course of their work-up was performed. mycobacterium tuberculosis was cultured in 68% of patients, and atypical myco ... | 1992 | 1575308 |
| infectious arthritis in patients with rheumatoid arthritis. | eleven cases of infectious arthritis occurring in patients with rheumatoid arthritis are reported. staphylococcus aureus was the causative organism in eight patients. streptococcus anginosus and streptococcus agalactiae in one patient each, and mycobacterium tuberculosis in two patients. the mean duration of symptoms before diagnosis was 16 days in patients with pyogenic arthritis. the diagnosis of joint infection caused by mycobacterium tuberculosis was especially delayed (57 days). four patien ... | 1992 | 1575593 |
| [comparison of the bacter system and löwenstein-jensen medium for cultivation of mycobacterium tuberculosis in clinical samples]. | over a period of two years, 1364 clinical samples from pulmonary sources, were studied in order to compare the bactec-tb system with the löwenstein-jensen medium, for isolation of mycobacteria. within this period of time, 93 strains of mycobacterium tuberculosis were isolated, 71 (76%) were isolated by the bactec system, and 69 (74%) were isolated by the löwenstein-jensen. the number of contaminations was lower for the bactec system (3.2%) than for the löwenstein-jensen medium (5.3%). the mean t ... | 1992 | 1576185 |
| delayed type hypersensitivity (dth): quantitative studies of mycobacteria-induced destructive and non-destructive dth-reactions in the skin of guinea pigs. | research on delayed type hypersensitivity (dth) to facultative intracellular bacteria in guinea pigs focused especially on intracutaneous dth reactions. however, phenomena of erythematous and necrotic skin reactions were only evaluated qualitatively, dose-response relationships not investigated, differences between early and late reactions overlooked and the development of unspecific skin necrosis ignored. in this work, these aspects were investigated by characterisation of dth-mediated reaction ... | 1992 | 1576409 |
| fatal co-infection with pulmonary tuberculosis and herpes simplex virus 1 pneumonia under steroid treatment. | we report a case of adult respiratory distress syndrome in a 65 yr old man under inappropriate steroid therapy who developed both herpes simplex virus 1 (hsv) and mycobacterium tuberculosis pneumonia; we conclude from clinical and histological data that hsv 1 played a major role in the fatal outcome of this immunocompromised patient, in spite of acyclovir treatment. | 1992 | 1577130 |
| radioimmunoscintigraphic approach for the in vivo detection of tuberculomas--a preliminary study in a rabbit model. | radioimmunoscintigraphic approach could provide a viable non-invasive alternative for the diagnosis of deeply situated tuberculomas. we have evaluated this in a rabbit model constructed to give a characteristic localized tubercular lesion, with complimentary morphological, histological and antigenic profiles. 131i-anti myobacterium bovis (bcg) antibody was shown to localize at the lesion, where as 131i-bovine serum albumin and 99mtc-red blood cell scans were negative. the clearance of intraderma ... | 1992 | 1577614 |
| studies on biologically active complexes of cobalt(ii) and nickel(ii) with dithiooxamide-derived ligands. | a number of cobalt(ii) and nickel(ii) complexes with novel dithiooxamide ligands derived from precursors such as benzonitrile, benzosulfonamide, benzaldehyde, benzoic acid, and phenol have been synthesized and characterized on the basis of their elemental analysis molar conductance, magnetic moment, h nmr and electronic and ir spectral data. these ligands and their complexes have been screened for their antibacterial activities against bacterial species staphylococcus aureus, streptococcus pneum ... | 1992 | 1578226 |
| strand displacement amplification--an isothermal, in vitro dna amplification technique. | strand displacement amplification (sda) is an isothermal, in vitro nucleic acid amplification technique based upon the ability of hincii to nick the unmodified strand of a hemiphosphorothioate form of its recognition site, and the ability of exonuclease deficient klenow (exo- klenow) to extend the 3'-end at the nick and displace the downstream dna strand. exponential amplification results from coupling sense and antisense reactions in which strands displaced from a sense reaction serve as target ... | 1992 | 1579461 |
| reversal of the decline in the incidence of tuberculosis in the austrian tyrol and vorarlberg in 1989. | the incidence of tuberculosis in the austrian tyrol and vorarlberg has been declining over decades. in 1989 a reversal in this trend was observed. the number of initial isolates of mycobacterium tuberculosis at the federal public health laboratory in innsbruck increased by 2.2% in 1990 (137 strains), as compared with 1989 (134 strains). the number of cases reported to the health authorities increased by 7.6%. this increase has continued in 1991, with 162 initial isolates registered. it seems tha ... | 1992 | 1580071 |
| genetics and resistance to tuberculosis. could resistance be enhanced by genetic engineering? | recent observations strongly suggest a significant role for genetic factors in innate resistance to infection by mycobacterium tuberculosis. this relation was discovered in a study of tuberculosis in arkansas nursing homes and was supported by data from three outbreaks of tuberculosis in two prisons. a person's resistance level was found to correlate with the region of his or her ancestry. ancestors of person's in the more resistant group tended to derive from densely populated areas and cities ... | 1992 | 1580452 |
| inhibition of tumor growth by the intralesional administration of interleukin-3 into mice implanted with solid tumors. | the antitumor activity of three interleukin-3 (il-3) preparations administered intralesionally into mice bearing syngeneic solid tumors was investigated. il-3 preparations used in this study included s-il-3, which was isolated from the culture fluid of murine inguinal lymph node cells stimulated with an arabinomannan extracted from mycobacterium tuberculosis (ssm), the culture fluid of wehi-3 cells (w-il-3) and recombinant il-3 (ril-3). when a 1,500 u/kg dose of s-il-3 or w-il-3 was injected int ... | 1992 | 1580549 |
| endometrial tuberculosis acquired by a health care worker in a clinical laboratory. | i describe a case of endometrial tuberculosis acquired by a microbiologist while she was working in a clinical laboratory. granulomatous endometritis was found, and mycobacterium tuberculosis was isolated. respiratory tract exposure from a faulty exhaust hood is the likely source of infection. endometrial tuberculosis and laboratory-acquired infections are briefly discussed. | 1992 | 1580757 |
| proliferation of peripheral blood mononuclear cells from rheumatoid arthritis patients to mycobacterial antigens. | proliferation of rheumatoid and control peripheral blood mononuclear cells (pbmc) to an antigenic acetone-precipitable extract from mycobacterium tuberculosis (mta) was investigated. cells were also stimulated with the recall antigen tuberculin ppd (purified protein derivative) and the mitogen okt3. controls had a significantly higher response to both mta and tuberculin ppd than ra patients. however, lymphocytes from patients who had had their disease for 3-10 years proliferated more vigorously ... | 1992 | 1582125 |
| the role of fiberoptic bronchoscopy for diagnosis of pulmonary tuberculosis in patients at risk for aids. | in patients with acquired immunodeficiency syndrome (aids)-associated pulmonary mycobacterium tuberculosis (mtb) (group 1), we analyzed whether the addition of transbronchial biopsy (tbb) and bronchial brushings augmented the diagnostic mtb yield over nonbiopsy sampling. positive acid-fast bacilli (afb) smears from combined sputum, bronchoalveolar lavage (bal), and washings were 30 percent compared with 37 percent when brushings and tbb were added (p = ns). the addition of tbb increased culture ... | 1992 | 1582273 |
| time course of anti-sl-iv immunoglobulin g antibodies in patients with tuberculosis and tuberculosis-associated aids. | immunoglobulin g (igg) and igm antibodies against the sl-iv antigen of mycobacterium tuberculosis in the sera of patients with tuberculosis with negative serology for human immunodeficiency virus (hiv) infection (tb group; n = 97), patients with tuberculosis with positive serology for hiv infection (tb-hiv group; n = 59), and healthy controls (n = 289) were determined by enzyme-linked immunosorbent assay. all sera were obtained at the onset of tuberculosis, i.e., when clinical symptoms appeared. ... | 1992 | 1583105 |
| use of serum antibody and lysozyme levels for diagnosis of leprosy and tuberculosis. | active tuberculosis (tb) and leprosy are difficult to diagnose early because there are few organisms to detect and the specific immune response does not distinguish between active and inactive disease. we developed an immunoassay for lysozyme to see whether serum lysozyme levels could be used to identify individuals with clinical leprosy or tb. the immunoassay for lysozyme proved superior to standard enzyme assays that were less sensitive and reliable. the lysozyme assay was compared with assays ... | 1992 | 1583106 |
| separation of mycobacterium bovis bcg from mycobacterium tuberculosis and mycobacterium bovis by using high-performance liquid chromatography of mycolic acids. | profile analysis of mycolic acid ester patterns of mycobacterium tuberculosis, mycobacterium bovis, and mycobacterium bovis bacillus calmette-gúerin (bcg) using high-performance liquid chromatography indicated that separation of bcg from m. tuberculosis and m. bovis by elution and relative retention times is possible. mycolic acid patterns of bcg eluted from the column 0.5 min before m. tuberculosis or m. bovis, resulting in relative retention times for two peaks not seen in the pattern of m. tu ... | 1992 | 1583141 |
| rapid, simple method for treating clinical specimens containing mycobacterium tuberculosis to remove dna for polymerase chain reaction. | several simplified methods for treating mycobacteria to release dna for amplification by the polymerase chain reaction (pcr) were investigated. the most effective of the methods was sonication. samples were placed in screw-capped microcentrifuge tubes that were then placed in a plastic rack. the rack was floated in a dish of water next to the ultrasonic probe so that the ultrasonic energy was transmitted through the walls of the tubes. this allowed multiple samples to be processed safely and eff ... | 1992 | 1583142 |
| tuberculous otomastoiditis. | a review of the 20th century english-language literature has produced a total of 320 cases of tuberculous otitis media or otomastoiditis, of which a surprising 93 cases have been reported in the 5-year period 1986-1990. eighty-three percent of these have come from indigenous african populations. the international health and sociological implications of this development are disturbing. three personal cases seen during the past 8 years are presented, all of whom came from the indigenous norwegian ... | 1992 | 1584595 |
| [isolation of mycobacteria from sputum. comparison of the mb check with the radiometric and conventional method]. | 1179 sputum samples were investigated by the mb-check, the conventional method and bactec 460 to compare the recovery rate of mycobacterium tuberculosis, the contamination rate, and time requirement for the isolation. of the 293 positive sputa, the mb-check detected 89,1%, löwenstein jensen medium 90,1%, the bactec-system 90,4%, the combination mb-check/löwenstein jensen 95,9%; and the combination bactec/löwenstein jensen 96,6%. the average detection times for 130 mycobacterium tuberculosis-stra ... | 1992 | 1584735 |