Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| human phagocytic cell responses to mycobacterium leprae and mycobacterium bovis bacillus calmette-guérin. an in vitro comparison of leprosy vaccine components. | components of current vaccines for hansen's disease include mycobacterium bovis bacillus calmette-guérin (bcg) and killed mycobacterium leprae. bcg infections in humans are rare and most often occur in immune-compromised individuals. m. leprae on the other hand, although not causing clinical disease in most exposed individuals, is capable of infecting and replicating within mononuclear phagocytes. lymphocytes from patients with the lepromatous form of hansen's disease exhibit defective lymphokin ... | 1988 | 3137262 |
| who expert committee on leprosy. | 1988 | 3140498 | |
| serological responses of patients with lepromatous and tuberculoid leprosy to 30-, 31-, and 32-kilodalton antigens of mycobacterium tuberculosis. | sera from patients with lepromatous and tuberculoid leprosy were examined in immunoblot assays for antibodies to mycobacterium tuberculosis culture filtrate antigens. antibodies to 30- and 31-kilodalton proteins were present in 88 and 81%, respectively, of 16 patients with lepromatous disease and absent in 16 patients with tuberculoid disease. antibodies to a 32-kilodalton protein were found in 12 and 38% of lepromatous and tuberculoid patients, respectively. these reactivities may be useful for ... | 1988 | 3141462 |
| relative cross reactivity of habanin, lepromin and tuberculin in guinea pigs sensitized with homologous and heterologous mycobacteria. | an atypical strain mycobacterium habana has been studied for its antigenic cross reactivity with delayed type of hypersensitivity responses in guinea pigs. guinea pigs sensitized with m. habana, m. leprae and m. tuberculosis when challenged with habanin, lepromin and tuberculin in criss-cross fashion have demonstrated strong cross reactivity with each other. possibilities of developing m. habana as a vaccine against tuberculosis and/or leprosy has been discussed. | 1988 | 3143770 |
| [correlation of skin tests and the production of gamma-interferon in leprosy patients]. | the responsiveness of 25 leprosy patients to an in vitro correlate of cell-mediated immunity to m. leprae antigen, (the production of y-ifn by peripheral blood mononuclear cells) was compared to their response to skin tests routinely used to evaluate cell-mediated immunity to a number of antigens including m. leprae. the results indicate that among the polar lepromatous patients (ll) there was a clear correlation between low responses in the in vitro assay (y-ifn production), negative skin test ... | 1988 | 3143869 |
| antigenic relatedness of a strongly immunogenic 65 kda mycobacterial protein antigen with a similarly sized ubiquitous bacterial common antigen. | in gene libraries of mycobacterium bovis bcg, mycobacterium tuberculosis and mycobacterium leprae recombinants were frequently found expressing an immunodominant 65 kda protein antigen. in this study polyclonal and monoclonal antibodies against the 65 kda antigen were found to react with a variety of different bacteria. furthermore it is shown that the 65 kda mycobacterial protein belongs to the family of antigens previously designated 'common antigen' due to their presence in a large variety of ... | 1988 | 3143894 |
| antibodies to hiv-1 in sera from patients with mycobacterial infections. | sera from 478 persons (348 leprosy patients, 33 tuberculosis patients, 29 healthy contacts of leprosy patients, 38 normal healthy indians, and 30 normal healthy europeans) were screened for anti-hiv-1 igg antibodies by elisa. none was positive. in addition, 132 samples (from 43 leprosy patients, 21 tuberculosis patients, 5 healthy contacts of leprosy patients, 33 normal healthy indians, and 30 normal healthy europeans) were also tested by western blot assay for anti-hiv-1 igg antibodies. only 1 ... | 1988 | 3146610 |
| mechanisms of mycobacterium leprae-specific t-cell deficiency in lepromatous leprosy. | patients suffering from lepromatous leprosy fail to develop an efficient cell-mediated immunity towards mycobacterium leprae, the causative agent. the mechanism of such a specific t-cell tolerance to the bacillus remains a key question in the pathophysiology of leprosy. macrophages do not show any intrinsic defect in phagocytizing and killing m. leprae or in presenting antigen to helper t-cells. on the other hand, m. leprae-reactive helper t-cells do persist in lepromatous patients, but their ac ... | 1988 | 3147697 |
| immunological responsiveness to m. leprae and bcg antigens in 98 leprosy patients and their household contacts. | 1. the cellular immune response to m. leprae and bcg antigens was evaluated in 98 leprosy patients and 143 household contacts lacking clinical manifestation of the disease. 2. the proliferative responses and release of interferon-gamma by peripheral blood mononuclear cells were assessed and both patients and contacts were classified as low or high responders to m. leprae. 3. the high responder contacts constituted 54.8% of the population analyzed, a three times higher proportion when compared to ... | 1988 | 3147795 |
| [peripheral nerve lesions of experimental leprosy in monkeys. iv. relationship between progress of histopathological findings and anatomical location of peripheral nerves]. | 1987 | 3149270 | |
| serum antibody responses to mycobacteria in leprosy patients and their contacts. | 1988 | 3149706 | |
| distribution of phthiocerol diester, phenolic mycosides and related compounds in mycobacteria. | among 28 mycobacterial species studied, only mycobacterium tuberculosis, m. bovis, m. africanum, m. marinum, m. kansasii, m. gastri and m. ulcerans produced waxes yielding long-chain beta-diol components (called phthiocerol and companions) and polymethyl-branched fatty acids on saponification. the same mycobacterial species also produced diesters of phenol phthiocerol and companions. fatty acids esterifying these fatty alcohols in m. marinum and m. ulcerans were found to belong to the phthiocera ... | 1988 | 3149973 |
| diphenoloxidase of mycobacterium leprae: discovery, applications and prospects. | 1988 | 3150974 | |
| metabolism in mycobacterium leprae, m. tuberculosis and other pathogenic mycobacteria. | 1988 | 3151445 | |
| towards new leprosy and tuberculosis vaccines. | leprosy and tuberculosis are chronic infectious diseases causing major global health problems, for which no effective control by vaccination has been achieved. recent advances in biotechnology may facilitate the design of a new vaccine generation. in this paper the need for, and the potential of, leprosy and tuberculosis vaccines are discussed. | 1987 | 3155274 |
| resistance to mycobacteria in mice treated with fractionated total lymphoid irradiation (tli) and in mice reconstituted with allogenic bone marrow cells following radiotherapy. | the increased clinical use of total lymphoid irradiation (tli) as an immunosuppressive adjunct in transplantation suggested the need for determining the effects of tli on the in vivo susceptibility of animals to infections controlled by cell-mediated immunity. tli-treated, tli-treated and splenectomized, and chimeric mice prepared with tli were inoculated in the hind foot pad with mycobacterium marinum or mycobacterium leprae. although m. marinum organisms multiplied in greater numbers in the tl ... | 1985 | 3155713 |
| reduction of tellurite and deesterification of fluorescein diacetate are not well correlated with the viability of mycobacteria. | both mycobacterium leprae and m. lepraemurium (mlm) were capable of reducing tellurium as tellurite ion (te4+) to elemental tellurium (te), seen by electron microscopy as fine crystals within the bacterial cells. there appeared to be close correspondence between the capacity to reduce tellurite, bright green fluorescence after staining with fluorescein diacetate (fda) and the ability of m. smegmatis to multiply in culture. likewise, there appeared to be correspondence between tellurite reduction ... | 1988 | 3179056 |
| effect of in vitro formed immune complexes on macrophage functions of mycobacterium leprae infected mice. | nonspecific macrophage functions were studied in mycobacterium leprae infected and preformed immune complex (ic) administered normal (ni) and thymectomized/irradiated (tri) mice at different time periods. uninfected controls given ic were also included. significant decrease in the chemotaxis, phagocytosis and bactericidal activities of macrophages obtained from infected groups compared to their controls were observed. phagocytic and chemotactic activities of macrophages were normal but intracell ... | 1988 | 3192973 |
| detection of phenolic glycolipid i of mycobacterium leprae and antibodies to the antigen in sera from leprosy patients and their contacts. | 1988 | 3195153 | |
| solubilization of preformed immune complexes in sera of patients with type 1 and type 2 lepra reactions. | serum complement activity in leprosy patients has been studied using solubilization of preformed immune complexes as an index. the solubilization capacity of sera from lepromatous patients with or without erythema nodosum leprosum (enl) as well as from type 1 reactional patients was found markedly reduced as compared to controls. solubilization did not improve at all in the enl patients after remission of the reaction phase. the addition of fresh normal sera failed to bring about any significant ... | 1988 | 3221112 |
| defining the magnitude of ocular complications from leprosy: problems of methodology. | a comprehensive review of all ocular surveys (40) of leprosy patients was undertaken. these surveys do not provide adequate information for defining the incidence or prevalence of ocular disease caused by mycobacterium leprae. furthermore, the level of disability and blindness from leprosy has not been addressed. the primary methodologic problems in these surveys are: a) lack of survey sampling techniques, b) institution-based or. clinic-based populations as the study population, c) inadequate i ... | 1988 | 3221113 |
| mycobacterium leprae-susceptibility of non-obese diabetic hybrid nude mice. | 1988 | 3250960 | |
| effect of multidrug therapy on the levels of antibodies to mycobacterium leprae glycolipid-1 in the leprosy spectrum. | sera from one hundred and forty five untreated leprosy patients, ten proven cases of active pulmonary tuberculosis and twenty five healthy volunteers not exposed to m. leprae infection were assayed for pgl-1 antibodies. all available follow up samples after multidrug therapy were also assayed. a decline in the level of pgl-1 antibodies were seen in many of the post-treatment samples, giving an indirect assessment of the bacterial load and the prognosis of the disease. | 1988 | 3253332 |
| corneal changes in nine-banded armadillos with leprosy. | leprosy is the third leading cause of blindness worldwide; however, little is known about the ocular changes that occur during the disease process. we have studied the eyes of two nine-banded armadillos with experimental mycobacterium leprae infection by light and electron microscopy. both animals had been inoculated intracutaneously, one 5 years and the other 2 years previously. light microscopy revealed invasion by acid-fast bacilli which were seen in keratocytes and mononuclear phagocytes in ... | 1988 | 3275592 |
| rifampicin-resistant strains of mycobacterium leprae may have reduced virulence. | a strain of mycobacterium leprae resistant to rifampicin (rmp) failed to infect normal mice when injected into the foot pads (fp) at a dose of 10 or 100 bacilli/fp, although it could be maintained by serial passage in mice by the use of inocula of 10(4) bacilli/fp; normal mice can be infected by rmp-sensitive m. leprae at a dose of 10 bacilli/fp. by contrast, nude (athymic) mice could be infected with an inoculum of 10 bacilli/fp of the rmp-resistant strain. it is suggested that the strain conce ... | 1988 | 3275779 |
| the 13th kellersberger memorial lecture, 1987. a view of vaccines against leprosy and a reflection on "appropriate science" and the third world. | 1988 | 3276511 | |
| clofazimine analogues active against a clofazimine-resistant organism. | clofazimine analogues active against a strain of mycobacterium smegmatis 607 made resistant to the antileprosy agent have been synthesized. activity (i.e., less than or equal to 2 micrograms/ml causing complete inhibition of growth) requires that there be a basic nitrogen in the "rimino" side chain and that the spacer distance between this nitrogen and the imino nitrogen be at least three carbon atoms. the nitrogen may be primary, secondary, or tertiary and may be part of an open chain or enclos ... | 1988 | 3279207 |
| measurement of hypoxanthine incorporation in purified suspensions of mycobacterium leprae: a suitable method to screen for anti-leprosy agents in vitro. | the rate of incorporation of hypoxanthine was measured in suspensions of mycobacterium leprae, with and without added anti-leprosy agents. dapsone, clofazamine and brodimoprim, as well as other benzylpryimidines, inhibited hypoxanthine incorporation, and their minimum inhibitory concentrations for incorporation with intact m. leprae were near the minimum inhibitory concentrations at which the agents have antibacterial effects. at sub-inhibitory concentrations for hypoxanthine incorporation, some ... | 1988 | 3279214 |
| immunological significance of mycobacterium leprae cell walls. | cell walls of mycobacterium leprae, prepared by differential solvent extraction, were shown to contain arabinogalactan, mycolates, and peptidoglycan. in addition, amino acid analysis revealed the unexpected presence of large amounts of protein that retained potent immunological reactivity. purified cell walls stimulated proliferation of t cells from tuberculoid, but not from lepromatous leprosy, patients and elicited delayed-type hypersensitivity skin reactions in guinea pigs and patients sensit ... | 1988 | 3279420 |
| heterologous expression of the 65-kilodalton antigen of mycobacterium leprae and murine t-cell responses to the gene product. | the gene encoding the immunodominant 65-kilodalton antigen of mycobacterium leprae was subcloned from a lambda gt11 clone into the high-copy-number plasmid puc8. escherichia coli containing these recombinants produced large amounts of the antigen, which was purified by polyacrylamide gel electrophoresis in the presence of urea. the ability of e. coli to recognize the mycobacterial promoter was confirmed by constructing additional clones in which the gene is flanked by transcriptional terminators ... | 1988 | 3281904 |
| a review of immunopathologic studies of human leprosy lesions in situ. | 1988 | 3284839 | |
| progress in the serology of leprosy at the university of hawaii. | 1988 | 3284840 | |
| relapse in hansen's disease. | 1988 | 3284841 | |
| responses to mycobacterium leprae by lymphocytes from new and old leprosy patients: role of exogenous lymphokines. | lepromatous leprosy patients generally have reduced response to mycobacterium leprae antigens in an in vitro lymphocyte transformation test, which could be due to insufficient generation of reactions or to active suppression of any reaction generated. we could detect 3 types of lack of reactivity: one which could be restored by the addition of supernatants from healthy, pha-stimulated lymphocyte cultures, one which could not thus be restored and one in which the culture supernatant contained fac ... | 1988 | 3285856 |
| immunoblotting and the immune response to leprosy. | immunoblotting has provided a powerful and effective approach to dissection of the immune response to mycobacterial antigens in a situation in which the availability of isolated antigenic components is severely limited. the basic approach of blotting on to a solid-phase support has been used in combination with sds/polyacrylamide-gel electrophoresis, t.l.c., recombinant dna technology and t-cell cloning in order to carry out a comprehensive analysis of glycolipid and protein antigens involved in ... | 1988 | 3286317 |
| leprosy and immunity: genetics and immune function in multiple case families. | genetic susceptibility to infection with m. leprae was studied in 10 multiple case families of australian aborigines. of the 87 members available for study, 24 had proven stable clinical leprosy which had been or was still being treated with diamino diphenyl sulphone. evidence of contact with m. leprae in the remaining 63 members as assessed by elisa to m. leprae sonicate and phenolic glycolipid (pgl) or by indirect immunofluorescence antibody assay was found in 78%, 64% and 71%, respectively. b ... | 1988 | 3286485 |
| antigenic profile of icrc bacilli with special reference to isolation of immunogenic subunit. | a vaccine containing icrc bacilli induces persistent immune conversion in lepromatous leprosy (ll) patients and lepromin-negative healthy subjects, in association with upgrading of the tissue responses in the former. with an idea to isolate the immunogenic 'subunit(s)', antigenicity of icrc sonicate and its fractions were tested, with reference to both b- and t-cell responses. a very high molecular weight glycolipoprotein, named pp-i with an apparent molecular weight of 1,000,000, has been isola ... | 1988 | 3286519 |
| skin smears and the bacterial index (b) in multiple drug therapy leprosy control programs: an unsatisfactory and potentially hazardous state of affairs. | 1988 | 3286795 | |
| allergy and immunity in leprosy: are these concepts becoming obsolete? | 1988 | 3286796 | |
| determination of the d and l configuration of phenolic glycolipids of m. leprae and m. bovis. | 1988 | 3286797 | |
| effect of shipment of skin-biopsy specimens to a distant laboratory on viability of mycobacterium leprae. | 1988 | 3286798 | |
| vaccination of human volunteers with heat-killed m. leprae: local responses in relation to the interpretation of the lepromin reaction. | the early (fernandez) and late (mitsuda) lepromin reactions were closely examined in a group of healthy, bcg-vaccinated individuals who were given four doses of a heat-killed, armadillo-derived vaccine, i.e., 1.5 x 10(7), 5 x 10(7), 1.5 x 10(8), and 5 x 10(8) bacilli. there was a clear dose-response relationship for both the early and late reactions with no leveling of the responses within the range of doses examined. while the early response was negative in most of the volunteers, the late resp ... | 1988 | 3286799 |
| soluble antigen of m. leprae coupled with liposomes elicits both "early" and "late" delayed hypersensitivity skin reactions. | the soluble antigen(s) of mycobacterium leprae was(were) coupled to liposomes and used for skin testing of leprosy patients, hoping that this mode of antigen presentation would be identical to that of integral lepromin. the liposomized antigen(s) elicited both early (24-48 hr) and late (3-4 weeks) delayed-type hypersensitivity reactions, true to the nature of lepromin, unlike the soluble antigen(s) alone which elicit(s) only the early reaction. | 1988 | 3286800 |
| macrophages, myeloperoxidase, and mycobacterium lepraemurium. | 1988 | 3286810 | |
| [case of lepromatous leprosy]. | 1988 | 3289278 | |
| chemotherapy of leprosy. | 1988 | 3289485 | |
| antigens of mycobacterium leprae identified by immunoprecipitation with sera from leprosy and tuberculosis patients. | mycobacterial antigens which react with human b lymphocytes were investigated by immunoprecipitation of radiolabelled sonicates of mycobacterium leprae and m. bovis (bcg) with sera from patients with leprosy and tuberculosis in the presence of staphylococcus aureus. sds-page analysis of the immunoprecipitates demonstrated that dense bands of mr 12,000 (12k), 15k, 27k, 32-33k, 36k and 48k were the major antigens of m. leprae recognized by antibodies in lepromatous leprosy sera. of these, only the ... | 1988 | 3289801 |
| biophysical optima for metabolism of mycobacterium leprae. | the metabolic response of freshly harvested, nude-mouse-derived mycobacterium leprae to biophysical parameters was studied to facilitate an understanding of axenic culture requirements. quantitation of intracellular atp and the rate of [u-14c]palmitic acid incorporation into phenolic glycolipid i (pgl-i) were used as metabolic indicators after axenic incubation in modified dubos medium under various biophysical conditions. pgl-i synthesis was optimal at 33 degrees c, whereas atp was optimally ma ... | 1988 | 3290244 |
| human monoclonal antibodies to phenolic glycolipid-i derived from patients with leprosy, and production of specific anti-idiotypes. | human monoclonal antibodies (mab) were produced by hybridomas derived from fusion of the gm4672 lymphoblastoid cell line and peripheral blood mononuclear cells from leprosy patients. hybridoma supernatants were screened for immunoglobulin (ig) secretion, binding to mycobacterium leprae, phenolic glycolipid-i (phen gl-i), the unique m. leprae glycolipid and single-stranded(ss)dna by elisa. on the basis of direct-binding elisas, two igmk mab (pr4 and th3) were selected for characterization. pr4 an ... | 1988 | 3292409 |
| screening of drug resistant strains of mycobacterium leprae in lepromatous leprosy patients under multidrug treatment. | 1988 | 3294170 | |
| ultrastructure of human foetal schwann cells in tissue culture infected with mycobacterium leprae. | 1988 | 3294539 | |
| association of igg and igm antibodies to phenolic glycolipid-1 antigen of mycobacterium leprae with disease parameters in multibacillary leprosy patients. | 1988 | 3294540 | |
| characteristics of the multiplication of dapsone-resistant strains of mycobacterium leprae in mice. subcommittee on clinical trials of the c hemotherapy of leprosy (thelep) scientific working group of undp/world bank/who special programme for research and training in tropical diseases. | 1988 | 3294541 | |
| surveillance for naturally acquired leprosy in a nine-banded armadillo population. | samples from 77 nine-banded armadillos (dasypus novemcinctus) inhabiting a 16.7 km portion of the east atchafalaya river levee, point coupee parish, louisiana, were serologically tested and/or histopathologically examined for evidence of naturally acquired leprosy. five of 67 (7.5%) armadillos tested sero-positive with elisa test for igm class antibodies to the phenolic-glycolipid-1 antigen of mycobacterium leprae. one of 74 (1.3%) was histopathologically positive as determined by presence of ac ... | 1987 | 3295310 |
| concepts behind the development of multiple drug therapy regimens in leprosy. | 1986 | 3295437 | |
| beta-lactamase synthesis in mycobacterium leprae. | beta-lactam antibiotics are not active against mycobacterium leprae. the enzyme beta-lactamase mediates the most common form of bacterial resistance to penicillins and cephalosporins. cell-free extracts of purified suspensions of m. leprae were examined for beta-lactamase. the bacteria were prepared from the tissues of experimentally-infected nine-banded armadillos. most of the suspensions were inactive. however, the bacteria obtained from the tissues of armadillos treated with penicillin g benz ... | 1987 | 3295489 |
| mycobacterial antigen detection by immunohistochemistry in pulmonary tuberculosis. | a preliminary diagnosis of tuberculosis can be established by the detection of acid-fast bacilli (afb) and confirmed by culture of the microorganism. to evaluate an alternative method of diagnosis, the distribution of mycobacterial antigens in lung tissue specimens was characterized by an indirect peroxidase-antiperoxidase method and was compared to the detection of afb by ziehl-neelsen stain. histologic specimens were obtained from 59 hospital patients. of nine patients with mycobacterial disea ... | 1987 | 3297995 |
| activity of minocycline in mycobacterium leprae-infected mice. | 1987 | 3298454 | |
| assessment of the immune deficit in leprosy patients and the effect of recombinant il-2 in vitro. | although the mechanism of immunologic unresponsiveness in lepromatous leprosy remains unknown, it has been shown that interleukin-2 (il-2) production is defective in these patients. peripheral blood mononuclear cells (pbmc) were isolated from treated (less than 16 months) and untreated leprosy patients as well as household contacts; age, sex, ethnically matched control subjects; and laboratory staff. pbmc were cultured for 6 days with sonicated mycobacterium leprae (1-10 micrograms/ml), dharmend ... | 1987 | 3298472 |
| defective monocyte chemotaxis in active lepromatous leprosy. | this study of monocyte chemotaxis in leprosy patients showed a significant inverse correlation (p less than 0.05) of chemotaxis and the bacterial index (bi) (n = 22). in addition, there was a significant inverse correlation (p less than 0.05) between chemotaxis and the serum levels of anti-phenolic glycolipid-i igm antibodies (n = 20). patients taking thalidomide who had a bi greater than or equal to 1 had a significantly greater (p less than 0.001) chemotaxis response than that of patients with ... | 1987 | 3298473 |
| sensitivity and specificity of the fla-abs test for leprosy in mexican populations. | the epidemiological surveillance for leprosy must include several clinical and laboratory procedures. the fla-abs test of abe could be a useful tool for this purpose because it allows the demonstration of an effective contact with mycobacterium leprae. in order to establish the specificity, sensitivity, and predictability of the fla-abs test under mexican conditions, we studied sera collected from six groups of individuals: 60 healthy donors from a nonendemic area, 57 cases hospitalized for cond ... | 1987 | 3298474 |
| immunization of mice with mycobacterium leprae/mycobacterium bovis bcg admixtures: modulation of the acquired response to bcg. | the generation of acquired immunity to bcg in mice was compared to that in animals receiving an admixture of bcg and killed mycobacterium leprae. no significant qualitative differences were observed between the two groups in terms of their generation of delayed sensitivity, of protective t cells, and of immunological memory. in addition, the admixture was as effective as bcg in conferring protective immunity against certain nontuberculous mycobacteria, and in one case, that of m. avium 706, sign ... | 1987 | 3298475 |
| ability of the phenolic glycolipid-i antigen of m. leprae to elicit a positive mitsuda response in the armadillo (dasypus novemcinctus). | three lepromin-positive armadillos and three lepromin-negative armadillos were tested intradermally with 100 micrograms of phenolic glycolipid-i (pgl-i) in 0.1 ml of normal saline. positive delayed-hypersensitivity granulomas at 21 days in the lepromin-positive animals and negative responses in the lepromin-negative animals were obtained. these observations suggest that purified pgl-i is capable of eliciting cell-mediated immune or delayed-hypersensitivity responses in animals sensitized to myco ... | 1987 | 3298476 |
| activity of selected beta-lactam antibiotics against mycobacterium leprae. | twelve beta-lactam antibiotics were tested for activity against mycobacterium leprae growing in the foot pads of mice. two cephalosporins (7-aminocephalosporanic acid and cefuroxime) and one cephamycin (cefoxitin) showed significant activity against m. leprae, and one penicillin (mezlocillin) exerted possible growth-promoting activity. these results suggest that particular molecular structures may be required for activity against m. leprae. | 1987 | 3298478 |
| effect of interleukin-1 (il-1) and il-2 on lymphocytes from patients with leprosy. | 1987 | 3298481 | |
| an unusual case of untreated lepromatous leprosy with rare bacilli: an immunologic follow-up. | 1987 | 3298482 | |
| effect of preformed immune complexes on the course of mycobacterium leprae infection in normal mice. | 1987 | 3298483 | |
| mycobacterium leprae, probably a microbe-dependent microorganism. | 1987 | 3298484 | |
| affinity of mycobacterium leprae with lewis rat schwannoma cell line (lewis tc 98). | 1987 | 3298903 | |
| characteristics of patients in the thelep trials of chemotherapy of leprosy at bamako and chingleput. subcommittee on clinical trials of the chemotherapy of leprosy (thelep) scientific working group of the undp/world bank/who special programme for research and training in tropical diseases. | 1987 | 3298905 | |
| [testing of the footpad technic for the cultivation of mycobacterium leprae in different lines of mice populations in cuba]. | 1986 | 3299546 | |
| environmental nonhuman sources of leprosy. | leprosy has been considered to occur only after exposure to a human case. however, evidence has been accumulating that this conventional view is wrong and that an environmental nonhuman source is critical to some human infections with mycobacterium leprae. observations, some of which date back to the nineteenth century, support soil, vegetation, water, arthropods, and armadillos (dasypus novemcinctus) as environmental sources of leprosy. disparate clinical, epidemiologic, and microbiologic evide ... | 1987 | 3299637 |
| leprosy: cause, transmission, and a new theory of pathogenesis. | leprosy is generally accepted as being caused by mycobacterium leprae, an acid-fast organism often present in great numbers in certain forms of leprosy. however, it has not been possible to confirm with scientifically acceptable evidence that this entity is the cause of leprosy; laboratory cultivation, an essential factor in the proof, has not been accomplished with the acid-fast bodies seen in leprotic tissue. the mechanisms of transmission of the disease also remain conjectural; prolonged, clo ... | 1987 | 3299638 |
| primary dapsone resistance in multibacillary leprosy among nepalese children. | 1987 | 3299724 | |
| rapid in vitro metabolic screen for antileprosy compounds. | measurement of intracellular atp of mycobacterium leprae after direct in vitro exposure to antimicrobial agents was evaluated as a rapid means of identifying potentially useful therapeutic agents. nude mouse-derived m. leprae was incubated in an axenic modified dubos medium in the presence or absence of antimicrobial agents for up to 3 weeks. atp was then assayed by using the firefly bioluminescence technique. rifampin, clofazimine, and ethionamide each effected a significantly accelerated rate ... | 1987 | 3300539 |
| chemical synthesis of the trisaccharide unit of the species-specific phenolic glycolipid from mycobacterium leprae. | o-(3,6-di-o-methyl-beta-d-glucopyranosyl)-(1----4)-o-(2,3-di-o-methyl- alpha-l-rhamnopyranosyl)-(1----2)-3-o-methyl-l-rhamnopyranose, the haptenic trisaccharide of the mycobacterium leprae-specific phenolic glycolipid i (pgl-i) antigen, and related trisaccharides, were synthesized by allylation of o-2 of benzyl 4-o-benzyl-alpha-l-rhamnopyranoside using phase-transfer catalysis, methylation of the product, deallylation, and coupling to o-(2,4-di-o-acetyl-3,6-di-o-methyl-beta-d-glucopyranosyl)-(1- ... | 1987 | 3300972 |
| concepts behind the development of multiple drug therapy regimens in leprosy. | 1986 | 3301333 | |
| transmission of leprosy. | 1987 | 3302059 | |
| seroepidemiological studies in subsided cases of multi and paucibacillary types of leprosy using fla-abs test. | fla-abs test was carried out in 145 subsided cases of multibacillary (bl/ll) and 197 subsided cases of paucibacillary (tt/bt/i) types. the period of subsidence varied from 0-5 years or more. it was observed that percentage positivity decreases with the subsidence of the disease. in multibacillary cases, fla-abs positivity was 95% (active cases), 50% (after 1 year of subsidence) and 36% (after 5 years of subsidence) whereas in paucibacillary types fla-abs positivity was 80% (active cases), 34% (a ... | 1987 | 3302060 |
| skin smear examination in relation to multidrug therapy campaigns. | 1987 | 3302062 | |
| bacterial index: ridley's vs. dharmendra's scale. | 1987 | 3302063 | |
| studies of reactivity of some sri lankan population groups to antigens of mycobacterium leprae. i. reactivity to lepromin a. | 1987 | 3302577 | |
| seroreactivity against the mycobacterium leprae phenolic glycolipid i in mycobacteria infected or stimulated groups of individuals. | 1987 | 3302578 | |
| india carries out large-scale tests of anti-leprosy vaccine. | 1987 | 3302728 | |
| the relationship between reactivities to lepromin a (fernandez and mitsuda) and a soluble protein antigen of mycobacterium leprae. | three methods of evaluation were used to investigate the relationship between skin test reactions elicited by different antigens of mycobacterium leprae. the latter were the fernandez and mitsuda reactions to lepromin, and that to a soluble protein antigen (spa) of m. leprae. all three methods of evaluation demonstrated some degree of relationship though not as high as would be expected. the closest correlation was between mitsuda and spa reactions; while fernandez and mitsuda, and fernandez and ... | 1987 | 3304321 |
| dominant cell wall proteins of mycobacterium leprae recognized by monoclonal antibodies. | a cell wall fraction of mycobacterium leprae has enhanced potency in activating immune t cells. by using a panel of monoclonal antibodies (moab), the dominant immunogen in this preparation was shown to be a complex of proteins of apparent molecular weight (mr) 65 to 50 kd with a major antigen of 65 kd. antigen capture assays supported the results of immunoblots and elisa that this protein was concentrated in the cell wall. by varying the moab used as capture or tracer antibody, one of the three ... | 1987 | 3304738 |
| a cooperative taxonomic study of mycobacteria isolated from armadillos infected with mycobacterium leprae. | seventeen strains of mycobacteria, recovered from six armadillos experimentally infected with mycobacterium leprae, were examined in ten different laboratories. this collaborative study included use of conventional bacteriological tests, lipid analyses, determination of mycobactins and peptidoglycans, characterization by py-ms, and immunological, metabolic, pathological and dna studies. these armadillo-derived mycobacteria (adm) formed five homogeneous groups (numbered adm 1 to 5) on the basis o ... | 1986 | 3305778 |
| [resistance of mycobacterium leprae to dapsone and rifampicin: apropos of a survey carried out in the cape verde region (senegal)]. | since 1983, primary resistance of m. leprae to dds and rifampicin has been evaluated in new cases of lepromatous leprosy observed in the cap-verde region in senegal. out of the 13 strains isolated, 10 (77%) have been found resistant to dds, 7 at low level, 2 at intermediate level, 1 at high level; all of them have been found sensible to rifampicin. similar results have been obtained with 57 strains isolated from patients not yet treated coming from different geographical areas, seeing that 37, i ... | 1987 | 3306248 |
| [genetic component of the immune response to mycobacterium leprae in healthy subjects]. | 1986 | 3306256 | |
| immunopurification of radiolabelled antigens of mycobacterium leprae and mycobacterium bovis (bacillus calmette-guerin) with monoclonal antibodies. | radiolabelled sonicate of mycobacterium leprae when examined by sds-page and two-dimensional gel electrophoresis (2-de) contained fewer antigens than the comparable sonicate from m. bovis (bacillus calmette-guerin) (bcg). a solid-phase immunopurification assay with anti-m. leprae monoclonal antibodies (moab) was used to characterize four of these antigens. three of the moab were m. leprae-specific and with them antigens with apparent molecular weights (mr) of 12,000 (12k), 18k, and 35k were isol ... | 1987 | 3306906 |
| suppression of delayed hypersensitivity skin reactions to tuberculin by m. leprae antigens in patients with lepromatous and tuberculoid leprosy. | delayed hypersensitivity skin reactions to tuberculin when injected alone or in mixture with antigens of m. leprae were examined in leprosy patients and in healthy controls. the tuberculin reaction was significantly inhibited in more than one half of both ll and bt patients by the soluble extract of m. leprae (leprosin), the leprosin derived 12 kd protein or leprosin depleted of the 12 kd antigen. however, suppression was not found in healthy controls from a leprosy endemic region. these results ... | 1987 | 3308210 |
| limiting dilution analysis of the human t cell response to mycobacterial antigens from bcg vaccinated individuals and leprosy patients. | the number of peripheral blood t lymphocytes responding to soluble mycobacterial antigens from mycobacterium tuberculosis purified protein derivative (ppd) and m. leprae (mls) was estimated by limiting dilution analysis. antigen-induced lymphocyte activation was measured by means of [3h]tdr incorporation on day 10 of culture in the presence of suboptimal concentrations of interleukin 2 (il-2). in the peripheral blood of bcg-vaccinated individuals from the uk, the frequency of t lymphocytes respo ... | 1987 | 3308217 |
| clinical applications of recent advances in immunology of leprosy. | 1987 | 3309081 | |
| vaccines in leprosy. | 1987 | 3309082 | |
| multi-drug therapy in leprosy. | 1987 | 3309084 | |
| metabolic studies on mycobacteria. iii. demonstration of key enzymes of tca cycle in m. leprae. | cell free extracts of armadillo derived m. leprae, m. phlei, m. smegmatis and normal armadillo liver were analysed for the two key enzymes of tca cycle. aconitase activity was assayed in the presence of inhibitor fluorocitrate and it was observed that cell free extracts from cultivable mycobacteria as well as aramadillo derived m. leprae had this enzyme activity and 66-82% of this activity was inhibited by 0.1 mm fluorocitrate. 74% of m. leprae derived enzyme activity was inhibited by fluorocitr ... | 1987 | 3309085 |
| metabolic studies on mycobacteria. v. a preliminary report on the atp synthesis by mycobacteria including m. leprae by using different substrates. | by deletion and addition of various substrates in sauton's and dubos media, an experimental system has been standardised in which the role of various nutrients in the energy synthesis of mycobacteria can be determined. by using this system with cultivable mycobacteria it was observed that glycerol and asparagine are the important ingredients for atp synthesis by mycobacteria. glucose further enhanced the atp synthesis and growth of these mycobacteria. in the media containing asparagine or glycer ... | 1987 | 3309086 |
| treatment of chronic erythema nodosum leprosum with cyclosporine a produces clinical and immunohistologic remission. | we have treated three leprosy patients suffering from chronic, steroid-dependent erythema nodosum leprosum (enl) with cyclosporine a (csa). excellent results were obtained in two patients. extra-cutaneous manifestations of the reactional state were completely suppressed, and the development of new skin lesions was sharply curtailed. immunohistologic abnormalities characteristic of active enl were corrected. lymphocyte subpopulations and anti-mycobacterial antibody levels in peripheral blood were ... | 1987 | 3309088 |
| primary visceral virchowian (lepromatous) hanseniasis. | a case of primary visceral virchowian hanseniasis is presented. the onset and symptoms of the disease made one think that it was a lymphoma because of the severe enlargement of the liver, spleen, and lymph nodes. biopsies of the liver, lymph nodes, and bone marrow revealed virchowian infiltration with acid-fast bacilli and globi. the skin was free of lesions and negative to bacilli, and there were no neural symptoms. | 1987 | 3309089 |
| an ultrastructural study of dermal nerves in early human leprosy. | skin biopsies from the cutaneous lesions of seven patients with indeterminate, bt, bl, and ll leprosy of less than 1 year's duration were examined by light and electron microscopy. inflammatory cells, which marked the location of mycobacterium leprae in bacilliferous cases (bl and ll) were most frequently and consistently found in relation to dermal blood vessels, neurovascular bundles, nerves, arrector pili muscles, and skin adnexa. the number of bacilli and inflammatory cells in the epineurium ... | 1987 | 3309090 |