Publications

[effects of prostaglandin in an experimentally-induced acute hepatic failure model].		1987	3441033
the protective effect of cyclosporin a on experimentally-induced acute hepatic injury in mice.	when heat-killed propionibacterium acnes (p. acnes) and a small amount of endotoxin lipopolysaccharide (lps) were intravenously injected into mice at a week's interval, most of them died of massive hepatic cell necrosis. this experimentally-induced acute liver injury was significantly inhibited by cyclosporin a (csa), resulting in a remarkable improvement of the survival rate. this protective effect of csa on acute liver injury was also histopathologically confirmed. to study the mechanism by wh ...	1987	3443255
chondroitin sulfate-depolymerizing activity in streptococcus intermedius and other streptococci.	the type strain (atcc 27335) and 18 human oral isolates of streptococcus intermedius and some other related streptococcal species were tested for chondroitin sulfate c-depolymerizing activity employing a modified screening plate method of smith and willett. as the results, s. intermedius strains except for atcc 31412 strain were found to possess this activity. propionibacterium acnes atcc 11828 used as a positive control strain demonstrated strong activity, whereas s. intermedius strains showed ...	1987	3444428
bacterial flora of the conjunctiva at birth.	in the largest study to date of bacterial flora in newborns, we cultured the conjunctivae of 100 infants within 15 minutes after vaginal delivery and before any antimicrobial agents had been applied to the eye. all cultures were intensively analyzed for anaerobic and aerobic bacteria. by far the largest group of bacteria isolated were microaerophilic, such as lactobacillus species and diphtheroids, accounting for 46.8% of positive cultures and 62.3% of all bacteria isolated. the second largest g ...	1986	3454370
active immunization of rats against two tumoral ascites using live cells associated with corynebacterium parvum.		1987	3455654
nmr studies of biosynthetic pathways.		1986	3460493
frequency distributions of periodontal attachment loss. clinical and microbiological features.	the present investigation attempted to determine if the pattern of past periodontal destruction could be concisely summarized, and related to other clinical and microbiological parameters. 61 subjects between the ages of 12 and 61 years with destructive periodontal disease were evaluated at 6 sites per tooth for redness, plaque, suppuration, bleeding on probing, pocket depth, and attachment level. the frequency distribution of baseline attachment level measurements was computed for each individu ...	1986	3462210
the ontogeny of pulmonary alveolar macrophages in parabiotic mice.	a bilateral distribution of labeled blood monocytes was achieved by unilaterally labeling donor members of surgically parabiosed b6d2f1/j mice with tritiated thymidine (3htdr). labeled pulmonary alveolar macrophages (pam) were found in lung lavage fluid of donors. by contrast, no labeled pam were found in lung lavage fluid from unstimulated recipients, even 12 days after labeling. contralateral 3htdr flash labeling showed that in recipients 3%, or about 10(4), pam were in s phase of the cell cyc ...	1986	3462284
comparison of the effect of viral paramunity inducers pind-avi and pind-orf with that of bcg, corynebacterium parvum and levamisole on the growth of radiation-induced murine osteosarcoma.		1986	3463079
role of pulmonary macrophages in resistance to experimental metastasis.	intranasal inoculation of c3h/hen mice with propionibacterium acnes activates pulmonary macrophages but not splenic or peritoneal macrophages. when mice so treated were injected iv with tumor cells, no protection against the challenge was seen. conversely, inoculation of c3h/hen mice with p. acnes by the ip route activated splenic and peritoneal macrophages but not pulmonary macrophages. when mice with activated splenic and peritoneal macrophages were challenged with an iv injection of tumor cel ...	1986	3464675
bacterial aspects of chronic blepharitis.	patients with all forms of chronic blepharitis were thoroughly evaluated. these patients were found to have evidence for a primary bacterial component in the disease process only in the clinical staphylococcal and mixed seborrheic/staphylococcal forms of chronic blepharitis. evidence was found for a shared common pathway for staphylococcus aureus, coagulase negative staphylococci, and propionibacterium acnes to contribute to the disease process. these organisms were found to produce lypolytic ex ...	1986	3466455
modulation of the pattern of development of experimental disseminated leishmaniasis by corynebacterium parvum.	balb/c mice are extremely susceptible to leishmania major. in this mouse strain, the parasite multiplies progressively and rapidly disseminates to distant visceral and cutaneous sites. the present studies show, however, that if balb/c mice are infected with the l major as an admixture with formalin-killed corynebacterium parvum, they rapidly acquire the capacity to restrain the multiplication of the parasite at the site of inoculation, and prevent its dissemination to distant visceral sites. mor ...	1987	3468194
in-vitro sensitivity of bacteroidaceae, clostridia and propionibacteria to newer antimicrobial agents.	the susceptibility of 220 fresh clinical isolates of bacteroidaceae, clostridia and propionibacteria to imipenem, ceftazidime, ciprofloxacin and four further substances were established by agar dilution tests. imipenem was unique among the seven test antibiotics in that it was active against all the bacteria investigated. whereas clindamycin suppressed all gram-positive and 94 x 8% of the gram-negative strains tested, and metronidazole was effective against all but the propionibacteria, ceftazid ...	1986	3469196
in vitro study of the antibacterial activity of ofloxacin against recent clinical isolates.	the in vitro activity of ofloxacin, a new broad-spectrum antimicrobial agent, was studied by a standardized single disc method. a total of 990 clinical isolates were tested, including 20 strains of anaerobic bacteria. ofloxacin was highly active against 683 strains (70.41%), had intermediate activity against 109 (11.23%) and had no activity against 178 (18.35%). ofloxacin was highly active against e. coli, klebsiella sp., citrobacter sp., proteus mirabilis, proteus morganii, salmonella sp., camp ...	1987	3476290
biosynthesis of vitamin b12: concerning the origin of the methine protons of the corrin nucleus.	13c nmr spectroscopy has been used to locate six deuterium atoms incorporated biosynthetically on the periphery of the corrin nucleus of vitamin b12 (cyanocobalamin) derived from cells of propionibacterium shermanii grown in a medium containing 50% 2h2o and 13c-enriched delta-aminolevulinic acid. the implications of these results for the mechanism of vitamin b12 biosynthesis are discussed, and it is concluded that the same oxidation level of the intermediates is maintained throughout the biosynt ...	1987	3477792
management of infectious complications of intraventricular reservoirs in cancer patients: low incidence and successful treatment without reservoir removal.	at the time of analysis, the first 30 patients with ommaya reservoirs (or) at the children's orthopedic hospital and medical center in seattle, washington had had 32 reservoirs for a mean duration of 28 months. in all, the reservoir chambers had been punctured for either diagnostic or therapeutic purposes a total of 1,287 times with a mean of 40 injections per reservoir and 1.4 injections per month. six reservoir infections were diagnosed in five patients--a rate of one infection for every 153 r ...	1987	3480777
anaerobic endophthalmitis caused by propionibacterium acnes.		1986	3484611
augmentation of nk activity and/or macrophage-mediated cytotoxicity in the liver by biological response modifiers including human recombinant interleukin 2.	administration of several biological response modifiers (brms) to mice strongly augmented natural killer (nk) activity of leukocytes isolated from the liver. this augmentation of nk activity was induced by two synthetic molecules (mve-2 and poly iclc), by two brms of bacterial origin (formalin-fixed propionibacterium acnes: p. acnes and a streptococcal cell wall preparation designated ok-432), as well as a single injection of human recombinant interleukin-2 (hril 2). all of these brms augmented ...	1986	3484675
propionibacterium avidum as the etiologic agent of splenic abscess.	propionibacterium avidum was isolated from an intrasplenic abscess in a patient recovering from coronary artery bypass surgery. this organism has not previously been reported as an etiologic agent of splenic abscess nor has splenic abscess been described as a complication of coronary bypass surgery. this report emphasizes the potential pathogenicity of normal microbial flora following surgical manipulation.	1986	3486741
role of t-cell subsets in the destruction of established metastases of 13762a rat mammary adenocarcinoma.	the 13762a rat mammary adenocarcinoma metastasizes with high frequency to regional lymph nodes and lungs. the intratumoral injection of corynebacterium parvum on day 7 followed by primary tumor excision on day 20 significantly prolonged survival and cured 10-40% of syngeneic f344 rats. established metastases were destroyed by the treatment, and strong and specific tumor rejection immunity was induced. the purpose of the present study was to determine if t-cells were required for the c. parvum tr ...	1986	3487670
incidence and growth of methylcholanthrene-induced tumors in mice with altered immunological status.	balb/c mice were treated s.c. with 3-methylcholanthrene (mca), and tumor incidence and growth were followed for 9 months. immunological status of mice was altered by various treatments. thymectomized, lethally irradiated, bone marrow reconstituted mice served as t-cell deficient recipients. in order to suppress natural killer (nk)-cell/macrophage functions some mice were injected with silica particles; to enhance these functions some mice were given corynebacterium parvum (cp). silica and cp wer ...	1986	3490910
chronic propionibacterium endophthalmitis after extracapsular cataract extraction and intraocular lens implantation.	we studied six cases of chronic, indolent intraocular inflammation that occurred after extracapsular cataract extraction and posterior chamber intraocular lens implantation. the inflammation was characterized by a delayed onset, and in three cases had the clinical appearance of a granulomatous iridocyclitis. cultures of intraocular specimens obtained from six eyes yielded propionibacterium; five yielded p. acnes. pleomorphic gram-positive bacilli consistent with propionibacterium were identified ...	1986	3491543
inflammation after cataract surgery.		1986	3491544
propionibacterium acnes endophthalmitis seven months after extracapsular cataract extraction and intraocular lens implantation.	we present a case of endophthalmitis caused by propionibacterium acnes seven months following extracapsular cataract extraction and intraocular lens implantation. the markedly delayed clinical course of endophthalmitis associated with this organism appears to be highly stereotypical. recognition and treatment of this complication of cataract surgery allows an excellent chance at cure.	1986	3493460
tumor-specific t-lymphocyte cytotoxicity enhanced by low dose of c. parvum.	three routes of immunotherapy with corynebacterium parvum (cp) on an ascitic friend virus-induced leukemia were evaluated. only the intraperitoneal route, which provided optimal contact between cp and tumor cells, showed prolonged mean survival time. greatest effectiveness was obtained with multiple injections of cp at weekly intervals and with small initial tumor load. of particular interest was that lower dosages of cp (5 and 25 micrograms) gave longer protection than dosages of 50 and 250 mic ...	1987	3494802
differential protein synthesis by murine peritoneal macrophages elicited by various stimuli.	protein synthetic patterns of murine peritoneal macrophages were analyzed by two-dimensional polyacrylamide gel electrophoresis (2d page) of 35s methionine-labeled proteins. while the protein synthetic patterns exhibited by resident, inflammatory, and activated macrophages had numerous common features that distinguished them from the other normal non-macrophage cell types examined, unique proteins also characterized each macrophage population from the others. the accumulation by resident macroph ...	1987	3496410
chronic postoperative endophthalmitis associated with propionibacterium acnes.	we present two cases of propionibacterium acnes endophthalmitis that confirm the association of this organism with a delayed onset and chronic and indolent intraocular inflammation following extracapsular cataract extraction with posterior chamber intraocular lens implantation. the clinical, microbiologic, and histopathologic features of these cases suggest that the organism can be sequestered in the capsular bag, which may make this entity difficult to diagnose. we conclude that complete capsul ...	1987	3498474
anaerobic bacterial endophthalmitis.	eighteen patients with endophthalmitis involving anaerobic bacteria are presented. endophthalmitis followed cataract surgery in seven patients, penetrating trauma in six, a corneal graft in two, and an infected filtering bleb in two; there was one case of endogenous endophthalmitis. propionibacterium acnes was the most frequent anaerobe isolated (78% of cases). thirty-two percent of the patients had polymicrobial infection with mixed aerobic and anaerobic species. six cases of acute p. acnes end ...	1987	3498919
endophthalmitis associated with sequestered intraocular propionibacterium acnes.		1987	3499078
localized endophthalmitis: a newly described cause of the so-called toxic lens syndrome.	we report five cases of post-extracapsular cataract extraction infection in which subsequent pathologic analyses identified the organisms and found the infection to be localized or confined to the lens capsular sac. the most common offending organisms were gram-positive pleomorphic bacilli. in one case, we were able to identify the bacteria as propionibacterium acnes. we designate this condition a localized endophthalmitis. it should be considered any time a persistent, smoldering, postoperative ...	1987	3499503
the intraocular environment and experimental anaerobic bacterial endophthalmitis.	anaerobic bacteria are prevalent in conjunctival flora but have not been adequately investigated as possible causes of endophthalmitis. the mean oxidation-reduction potential (eh) of the rabbit vitreous was found to be +25.1 mv, well within the limiting eh value of many anaerobes. there was an oxygen pressure gradient in the vitreous ranging from 2.1 mm hg immediately posterior to the lens to approximately 20 mm hg adjacent to the medullary ray. endophthalmitis was produced with pure cultures of ...	1987	3499883
circulating interleukin 1 and tumor necrosis factor during inflammation.	it is proposed that interleukin 1 (il 1) and tumor necrosis factor (tnf) alpha play central roles in the host's response to inflammation. yet circulating concentrations have not been frequently measured in many inflammatory states. serum levels of il 1 and tnf were evaluated in mice with a tumor, sterile inflammation, endotoxinemia, or generalized peritonitis where an acute-phase protein response was documented. in tumor-bearing mice, no il 1 or tnf could be detected despite marked increases in ...	1987	3501249
[effect of associated microflora on cobalamin genesis by propionibacterium shermanii].		1987	3508958
randomized study of corynebacterium parvum adjuvant therapy following surgery for (stage ii) malignant melanoma.	one hundred and fifteen patients with histologically proven primary cutaneous melanoma were entered into a randomized prospective study following surgical removal of clinically obvious regional metastases. the study started in 1977 and was completed in june 1982. fifty-eight patients (10 with regional skin metastases and 48 with regional node metastases) were in the control group and 57 (15 regional skin, and 42 regional node metastases) received adjuvant c. parvum. other clinical features were ...	1986	3512025
effect of macrophage activation on phagocyte-plasmodium interaction.	we investigated the effect of both immune and normal sera on the binding of free plasmodium berghei by resident and activated macrophages. resident macrophages bound plasmodia to a greater extent than did activated macrophages, regardless of treatment. resident macrophages bound free plasmodia, predominantly trophozoites, in the presence of normal serum by a mechanism inhibited by n-acetylglucosamine and n-acetylmannosamine. macrophages activated through treatment with propionibacterium acnes (" ...	1986	3512432
endophthalmitis following intraocular lens implantation: report of 30 cases and review of the literature.	bacterial endophthalmitis is a postoperative complication of intraocular lens implantation. a review of 30 cases showed that 77% occurred within seven days of initial cataract surgery and that all cases occurred within 32 days. common presenting symptoms and signs included pain localized to the involved eye, decreased visual acuity, conjunctival injection, anterior chamber inflammation, hypopyon, and absent or poor red reflex. diagnosis of an infectious etiology was made by aqueous and vitreous ...	1986	3513284
lack of biological significance of in vitro brugia malayi microfilarial cytotoxicity mediated by propionibacterium acnes ("corynebacterium parvum")-and mycobacterium bovis bcg-activated macrophages.	the effect of nonspecific activation of host macrophages by propionibacterium acnes ("corynebacterium parvum") or mycobacterium bovis bcg on brugia malayi microfilariae was determined by in vitro and in vivo studies. intraperitoneal injection of c. parvum or bcg stimulated peritoneal exudate cells, which were toxic to microfilariae. microfilariae were equally susceptible to damage by c57bl/6j or balb/cj peritoneal exudate cells. furthermore, inhibitors of oxidative metabolism and arginine supple ...	1986	3516877
bcg, in contrast to c. parvum, does not induce increased sensitivity to the toxic effects of indomethacin in mice.	treatment of mice with killed corynebacterium parvum (also designated propionibacterium acnes, p. acnes) or bacille calmette guerin (bcg) leads to modification of several of the same host systems. however, bcg, in contrast to c. parvum, did not induce increased sensitivity to the toxic effects of indomethacin in balb/c or c57b1/6 mice. in addition, treatment of mice with bcg did not interfere with the induction of sensitivity by c. parvum. therefore c. parvum must uniquely induce changes in host ...	1986	3519813
therapy of stage iii (optimal) epithelial carcinoma of the ovary with melphalan or melphalan plus corynebacterium parvum (a gynecologic oncology group study).	a randomized prospective therapy trial in patients with stage iii optimal epithelial carcinoma of the ovary was accomplished by the gynecologic oncology group. therapy with melphalan or melphalan plus immuno-adjuvant, corynebacterium parvum (c. parvum), was utilized as adjuvant treatment following surgical therapy. one hundred eight-five patients were eligible for evaluation with 87 patients in the melphalan group and 98 patients in the melphalan plus c. parvum group. the comparison of the treat ...	1986	3525343
a sequential study of hepatic structural damage following the intravenous injection of corynebacterium parvum.	sequential histological and fine structural changes were studied in liver from 4.5 min to 21 days after a single intravenous injection of corynebacterium parvum in the rat. a previously unreported effect of the micro-organism, the formation of eosinophilic inclusion bodies within hepatocytes, was observed between 15 min and 24 h of injection. these structures were most common in periportal hepatocytes at 2 h. the structural, enzyme- and immunohistochemical features of these bodies suggest that t ...	1986	3527581
substances with biological activity of vitamin b12 formed during cultivation of propionibacterium freudenreichii in the presence of precursors (short communications).	the papers of kolhouse et al. and cooper et al. described the occurrence of vitamin b12 analogues of unknown origin in blood serum. some of these analogues may be derived from slaughter cattle raised on feed supplemented with vitamins and minerals, as was observed by allen. herbert et al. found vitamin b12 analogues in multivitamin preparations produced in u.s.a., and kanazawa et al. in human liver, red cells and brain. it is not clear so far, if and how do vitamin b12 analogues interfere with v ...	1986	3528859
[in vitro activity of roxithromycin, new semisynthetic macrolide against obligate anaerobes].	the "in vitro" susceptibility to roxithromycin and three other macrolides of 236 anaerobes isolated from clinical samples in 1984/1985 was determined by an agar-dilution method on wilkins chalgren medium. 90% of gram positive cocci were susceptible to both roxithromycin and josamycin (mic less than 1 mg/l, whereas 1 mg/l erythromycin and 2 mg/l spiramycin were able to inhibit respectively 46 and 86% of the same tested strains. no resistance to the four macrolides was observed among eubacterium, ...	1986	3534717
propionibacterium acnes cerebral botryomycosis. the role of plastic embedding in the diagnosis of grain-producing infections.	botryomycosis is a chronic infection in which bacterial colonies, or "grains," occur in purulent exudate associated with draining sinus tracts or abscess formation. this condition usually is caused by staphylococcus and streptococcus, but other organisms occasionally have been implicated. this report describes the first case of botryomycosis caused by propionibacterium acnes, a diphtheroid that has been rarely associated with opportunistic disease. in this case, plastic embedding allowed the obs ...	1986	3535486
requirement of a properly acylated beta(1-6)-d-glucosamine disaccharide bisphosphate structure for efficient manifestation of full endotoxic and associated bioactivities of lipid a.	several synthetic acylated glucosamine monophosphates, with structures corresponding to the nonreducing or reducing moiety of the lipid a of the escherichia coli or salmonella minnesota type, and a synthetic compound corresponding to a biosynthetic disaccharide lipid a precursor (designated ia or iva) were examined for their endotoxic and related bioactivities in comparison with those of the synthetic and bacterial parent molecules, i.e., acylated beta(1-6)-d-glucosamine disaccharide bisphosphat ...	1987	3539807
regulation of i-a expression by murine peritoneal macrophages: differences linked to the bcg gene.	we previously reported that mycobacterium bovis (strain bcg) induces continuous i-a expression when injected into bcg-resistant strains of mice. we have extended this observation by showing that corynebacterium parvum also induces continuous i-a expression by macrophages from bcg-resistant but not bcg-susceptible mice. we have linked continuous expression to bcg resistance by using c.d2ityr mice, which are congenic with bcg-susceptible balb/c mice except for genes on a portion of chromosome 1, w ...	1987	3543128
protein degradation by human intestinal bacteria.	analysis of human gut contents showed that substantial quantities of soluble protein, ammonia and branched chain volatile fatty acids occurred throughout the large intestine [0.1-24.4 g (kg contents)-1, 7.7-66.0 mmol (kg contents)-1 and 1.5-11.1 mmol (kg contents)-1 respectively]. the presence of these metabolites suggested that substantial proteolysis was occurring. in vitro studies showed that casein and bovine serum albumin were partly degraded in slurries of human faeces over a 96 h incubati ...	1986	3543210
role of reactive oxygen intermediates in the hepatotoxicity of endotoxin.	administration of endotoxin (2.5 micrograms/mouse, iv) to corynebacterium parvum-pretreated (14 days earlier, 1 mg/mouse, i.v.) mice caused a rapid (90 min) decrease in liver cytochrome p450-dependent drug metabolism and an elevation of serum transaminase. the time course of the priming effect of c. parvum suggested that macrophages might be responsible for this sensitization to endotoxin. the antioxidant n-acetylcysteine (500 mg/kg) effectively protected against this depression of liver drug me ...	1986	3546193
the bacterial flora of the nasal cavity in healthy young men.	the nasal cavity of 97 young healthy men (applicants for the pilot education in the finnish air force) was examined. bacterial culture demonstrated one or more species of aerobic bacteria in all 194 nasal cavities examined and anaerobic bacteria in 76.5%. in ten per cent of the cultures bacteria were detected after enrichment only. the most common aerobic bacteria were staphylococcus epidermidis (79%), diphtheroids (41%) and staphylococcus aureus (34%). haemophilus influenzae was found in 5% and ...	1986	3547601
phase iii study on the treatment of women with cervical cancer, stage iib, iiib, and iva (confined to the pelvis and/or periaortic nodes), with radiotherapy alone versus radiotherapy plus immunotherapy with intravenous corynebacterium parvum: a gynecologic oncology group study.	a report on a study conducted under the auspices of the gynecologic oncology group on the treatment of women with advanced carcinoma of the uterine cervix (stages iib, iiib, iva) with radiotherapy alone versus radiotherapy plus immunotherapy (intravenous corynebacterium parvum) is presented. there were 283 patients considered evaluable for analysis. of these, 135 patients were randomized to radiotherapy plus c. parvum (120 patients received at least one course) and 148 were randomized to radioth ...	1987	3549476
immunomodulators and wound healing.	the synthetic immunomodulators muramyl dipeptide (mdp), thymopoietin pentapeptide (tp5), and cp-46,665 were examined for their effects on wound healing in mice. we found no differences in wound disruption strength between immunomodulator-treated animals and saline controls on days 11, 14, and 21. the only exception was with high-dose cp-46,665, which produced weakened wounds on day 14 (p less than 0.05) and 21 (p less than 0.01). cp-46,665 was further studied by injecting high and low doses 48 h ...	1987	3553610
the influence of killed propionibacterium granulosum on experimental infection with escherichia coli.	mice infected subcutaneously with 5 x 10(3) viable cells of escherichia coli atcc 25922 incorporated in liquified agar developed a systemic infection. increasing bacterial numbers could be recovered from the liver at several days following infection. ultimately, the animals died 6 days after infection. treatment of mice with 1 mg killed propionibacterium granulosum kp-45 lead to an increased spleen weight at day 7 after intraperitoneal injection. hence, the animals were highly susceptible to the ...	1987	3553883
[etiology of erysipelas].	bacterial cultures were taken from 31 patients by scarification from the dermal compartment of recent erysipelas foci. pathogenic organisms were isolated from the dermis while cultures from the skin surface remained sterile in 5 patients; streptococcus pyogenes was cultured in 2 of these patients and staphylococcus aureus in the other 3 patients. our findings indicate that staphylococcal erysipelas does exist and we suppose that staphylococcus aureus is able to induce erysipelas, above all in th ...	1987	3554775
[search for propionic acid bacteria in the human intestine].	the possibility of detecting propionic acid-producing bacteria in the intestine of healthy humans with a view to obtaining a strain which is physiologically most suitable for therapeutic purposes has been studied. the selective conditions for the isolation of propionic acid-producing bacteria from the large intestine have been experimentally established. analysis of 70 puncture biopsy specimens of parietal mucus and luminal contents from different sections of the intestine has not shown the pres ...	1987	3554850
sequence homology around the biotin-binding site of human propionyl-coa carboxylase and pyruvate carboxylase.	biotin-dependent carboxylases require covalently bound biotin for enzymatic activity. the biotin is attached through a lysine residue, which in a number of bacterial, avian, and mammalian carboxylases, is found within the conserved sequence ala-met-lys-met. we have determined the partial nucleotide sequence of cdna clones for human propionyl-coa carboxylase and pyruvate carboxylase. the predicted amino acid sequence of both these proteins contains the conserved tetrapeptide 35 residues from the ...	1987	3555348
down-regulation of insulin receptors in propionibacterium acnes-activated macrophages in the mouse.	intraperitoneal administration of propionibacterium acnes in cd-1 mice was associated with the reduction in number of insulin receptors in peritoneal macrophages (m phi), and with elevated levels of insulin in plasma and the peritoneal cavity. when insulin levels returned to normal, insulin receptors in p. acnes-m phi were still reduced. insulin appears to contribute significantly to the down-regulation of the m phi-insulin receptors during the early stage of activation. other biologically activ ...	1987	3556266
infectious complications of intraventricular reservoirs in cancer patients.	drug administration via an intraventricular reservoir is useful in the treatment of leukemic and carcinomatous meningitis that occurs in patients who have previously received lumbar intrathecal chemotherapy. the intraventricular route, however, is associated with a higher incidence of infectious complications compared with therapy given by the lumbar route. to characterize the infectious complications associated with such reservoirs, we reviewed the 10-year experience of the pediatric branch, na ...	1987	3562137
pleurodesis--testing the waters.		1987	3565922
viability of lyophilized anaerobes in two media.	viability of 15 species of anaerobes was followed after freeze-drying and storage for 1 year. organisms maintained in a 12% sucrose concentration in chopped meat carbohydrate broth survived longer and maintained higher viability counts than those organisms in double-strength skim milk.	1987	3568744
cell-to-cell interaction of streptococcus sanguis and propionibacterium acnes on saliva-coated hydroxyapatite.	cell-to-cell interaction (coaggregation) between propionibacterium acnes pk93 and streptococcus sanguis dl1 was measured on saliva-coated hydroxyapatite beads (sha) at bacterial concentrations between 1.3 x 10(6) and 6.7 x 10(8) cells per ml. four hundredfold more dl1 than pk93 cells adhered to the saliva-coated beads, and the adherence of s. sanguis was proportional to cell input. sha precoated with 3 x 10(8) dl1 cells bound 75 to 80% of available pk93 cells at all input amounts tested, up to a ...	1987	3570474
suppression of basal and corynebacterium parvum-augmented nk activity during chemically induced tumor development.	c3h mice were injected subcutaneously (s.c.) with a tumorigenic dose (150 micrograms/mouse) of 3-methylcholanthrene (mc), followed by a 24-h injection and subsequent weekly injections of corynebacterium parvum (cp) i.p. for a total of 100 days. basal and cp-augmented nk cell activities were measured in controls and treatment groups during pre-tumor and tumor development stages. basal nk activity in spleen, peripheral blood and lung tissue was enhanced by cp, but was suppressed by mc. a resulting ...	1987	3583504
fluorescence from pilosebaceous follicles.	fluorescence studies were performed on the extrusions from pilosebaceous follicles. pressure extractions produced follicle samples which showed fluorescence under wood's light. the samples were then analysed in a fluorometer giving corrected excitation spectra. the structured emission spectra achieved were interpreted as being due to porphyrins produced by propionibacterium acnes (p. acnes). details in the spectra showed close resemblance to spectra from cultured p. acnes cells. the emission spe ...	1987	3592747
combined immunostimulation (propionibacterium avidum kp 40) and anticoagulation (heparin) prevents metastatic lung and liver colonization in mice.	the antineoplastic activity of propionibacterium avidum kp-40 and its enhancement by anticoagulation with heparin was studied. in balb/c mice syngeneic sarcoma l-1 exclusively caused tumor colonization of the lungs. after neuraminidase treatment the organotropism of this tumor was changed, with tumor nodules developing in lung and liver. after single systemic application of propionibacterium avidum kp-40 the number of lung and liver colonies decreased evidently. combination of this immunomodulat ...	1987	3597521
splenomimetic effect of corynebacterium parvum in fulminant pneumococcemia.	the efficacy of corynebacterium parvum to stimulate splenic growth and to boost host survival was examined by using adult sprague-dawley rats in a highly spleen-sensitive model of fulminant pneumococcemia. rats were either treated (10 days or 1 hr before or 1 hr after) or not treated with c. parvum; were depleted of complement; underwent partial, total, or sham splenic resection; and then were challenged with either a low (2 x 10(2)) or a high (2 x 10(5)) dose of pneumococci. in the absence of c ...	1987	3598214
high hepatic natural killer cell activity in murine lupus.	this study demonstrates a profound elevation of nk activity, as measured by cytotoxicity to yac-1 targets in a 4-h incubation 51cr-release assay, of freshly isolated hepatic npc from both mrl/lpr and (nzb x nzw)f1 mice. this marked increase was not observed in splenic or peripheral blood nk. the hepatic nk were nonadherent, radioresistant, ly-1-,2-, and agm1+. furthermore, biologic response modifiers can further augment hepatic nk activity in these autoimmune strains.	1987	3598462
[effects of azelastine on an experimentally-induced acute hepatic failure model].		1987	3606396
thermoimmunotherapy of advanced neoplasms. a concept and preliminary results.	a rationale for the use of local hyperthermia (lh) in cases of advanced neoplasms as well as indications for its therapeutic application are discussed. lh applied alone is of limited use, only about 50% of patients with advanced neoplasms benefit from this therapy, with a complete remission (cr) rate of 10-15%. thus lh should be considered as a supplementary form of cytoreductive therapy and it should be combined with other antineoplastic modalities. clinical studies on thermoimmunotherapy (a co ...	1987	3607266
[mechanism of intrahepatic cholestasis induced by heat-killed propionibacterium acnes in the tuberculin-sensitized guinea pig].		1987	3613226
pathogenicity of propionibacterium acnes isolated from kawasaki disease patients--cytopathogenic protein (cpp) isolated from p. acnes culture filtrates and measurement of the antibody against cpp.		1986	3613504
intrapleural corynebacterium parvum.		1987	3651650
inhibitory effect of tjn-101 ((+)-(6s,7s,r-biar)-5,6,7,8-tetrahydro-1,2,3,12-tetramethoxy -6,7-dimethyl-10,11- methylenedioxy-6-dibenzo[a,c]cyclooctenol) on immunologically induced liver injuries.	tjn-101, which is a lignan component isolated from schisandra fruits, inhibits hepatotoxic chemicals-induced liver injuries. in this study, effects of tjn-101 on immunologically induced liver injuries were investigated in vivo and in vitro. when a small dose of lipopolysaccharide was injected into mice previously injected with heat-killed propionibacterium acnes, most of the animals died with acute hepatic failure which was produced by cytotoxic factors from activated adherent cells, and liver c ...	1987	3656775
a newly described complication of neodymium-yag laser capsulotomy: exacerbation of an intraocular infection. case report.		1987	3662900
different photoinactivation mechanisms in propionibacterium acnes for near-ultraviolet and visible light.		1987	3671513
identification of anaerobic bacteria using high-performance liquid chromatography.	20 species of the genera clostridium, bacteroides, fusobacterium and the type strains of peptostreptococcus anaerobius, peptococcus asaccharolyticus, veillonella parvula and propionibacterium acnes were examined for the production of volatile (vfa) and nonvolatile (nvfa) short-chain fatty acids using high-performance liquid chromatography (hplc) with a column for organic acids (aminex hpx-87h). 10 min are needed for sample preparation and the vfa and nvfa were detected simultaneously in a single ...	1987	3673341
the protective effects of prostaglandin e1 in an experimental massive hepatic cell necrosis model.	when a small amount of gram-negative lipopolysaccharide was intravenously injected into mice which had been injected with heat-killed propionibacterium acnes 7 days before, massive hepatic cell necrosis was induced and most of the mice died 24 hr later. however, when prostaglandin e1 was administered with lipopolysaccharide, remarkable improvements in the survival rate and in the histological changes of the liver were observed. in order to find out how prostaglandin e1 suppressed the induction o ...	1987	3679086
[role of anaerobic non-sporulating bacteria in infections of tissue and closed body spaces. analysis of 624 clinical specimens].		1987	3684773
intraarticular injection of propionibacterium acnes causes an erosive arthritis in rats.	formalin killed propionibacterium acnes, was injected intraarticularly into the stifle joint of rats. the reaction was assessed by radiologic and histologic examination. a single injection induced a marginally erosive synovitis that receded by day 30. a second injection administered in the same joint resulted in tissue destruction that was evident radiographically. treatment by dexamethasone and some disease modifying drugs, i.e. chloroquine, hydroxychloroquine, sodium aurothiomalate and cycloph ...	1987	3687581
in vitro activity of ampicillin plus sulbactam against anaerobes compared to ampicillin and cefoxitin.	the antimicrobial susceptibility of 195 recent clinical isolates of anaerobic bacteria was studied to ampicillin alone, ampicillin + 1 mg/l sulbactam, ampicillin + 5 mg/l sulbactam, and cefoxitin by means of agar dilution tests. the ampicillin-sulbactam combinations were the most effective drugs against species of the bacteroides fragilis group, the mic90 of ampicillin + 5 mg/l sulbactam for b. fragilis being less than 1 mg/l, compared to 256 mg/l of ampicillin, 4 mg/l of ampicillin + 1 mg/l sul ...	1987	3692611
infections after craniotomy: a retrospective study.	a retrospective study was performed to evaluate the rate of infection directly related to craniotomy. of 246 craniotomies performed in 1981, 234 could be evaluated. the overall incidence of infection was 8.1% (19 infections in 18 patients). the infection rate was 15.2% after insertion of a ventricular shunt (seven infections after 45 ventriculostomies). staphylococcus aureus, staphylococcus epidermidis and propionibacterium acnes were the most frequent isolated micro-organisms. we recommended a ...	1986	3701096
failure to boost the increased clearance of embolic tumor cells from the lung with dose scheduling: corynebacterium parvum studies.	initial suboptimal, optimal and supraoptimal doses of intraperitoneal corynebacterium parvum were followed by 'booster' doses injected intraperitoneally 5, 10 and 25 days later. mice were challenged intravenously 4-6 days after the booster doses with [125i]iurd-labeled b16 tumor cells and the extent of clearance of these cells from the lungs analyzed 4 h later. in a second experiment, mice were given booster doses of c. parvum on days 4, 7 and 10 and then challenged on day 14 with labeled b16 tu ...	1986	3703464
antitumor and immunologic effects of a pyridine-extracted fraction of propionibacterium acnes.	the antitumor and immunological effects of a pyridine extractable fraction of propionibacterium acnes were tested in a murine ovarian teratocarcinoma (mot) model. previous studies have demonstrated that tumor rejection in this model depends upon sequential activation of tumoricidal neutrophils (pmns) followed by cytostatic macrophages. the pyridine extract significantly prolonged the survival of mice challenged with 10(3) or 10(4) mot cells but had little impact on a 10(5) tumor inoculum. in viv ...	1986	3708631
cytolytic interactions between murine macrophages, tumor cells, and monoclonal antibodies: characterization of lytic conditions and requirements for effector activation.	because of recent successes in inducing the effective rejection of neoplasms in vivo by administration of monoclonal antibodies (mab), we analyzed lytic interactions in vitro that occur between macrophages and several combinations of tumor targets and mab that can lead to such successful immunotherapy. murine macrophages, interacting with mab of the igg1, igg2a, igg2b, and igg3 isotypes directed against sw-1116 carcinoma cells, destroyed the tumor targets efficiently over 24 to 48 hr in vitro. l ...	1986	3711663
[antitumor efficacy of activated macrophages against murine glioma cells].	a crucial manifestation of malignant gliomas is the regrowth of already-invaded neoplastic cells after surgical intervention. one possible approach for inhibiting such tumor growth is to utilize the tumoricidal potential of macrophages. in order to investigate the clinical application of this concept, peritoneal exudate cells (pec) activated in vitro and in vivo by immunomodulating agents were tested for cytotoxic activity against murine glioma (203-glioma) cells. as immunomodulating agents, hea ...	1986	3717955
microbiologic analyses of nasal polyp tissue.	nasal polyps from 40 patients were cultured within 2 1/2 hours after surgical removal to determine whether microorganisms were present. the first 20 polyps were cultured for aerobic and anaerobic bacteria, viruses, fungi, mycoplasmas, and mycobacteria. of these 20 polyps, eight were sterile by all tests, one grew cryptococcus albidus, one grew sporobolomyces, one had large numbers of peptostreptococcus micros and propionibacterium acnes, greater than 10(6) colony-forming units per gram (cfu/gm), ...	1986	3722630
therapeutic effect of endogenous tumor necrosis factor on ascites meth a sarcoma.	the therapeutic effect of endogenous tumor necrosis factor (tnf) on meth a ascites fibrosarcoma in mice was investigated. serum and peritoneal fluid from tumor bearing mice treated with ok-432 and lps were cytotoxic to tumor cells in vitro. the peak of cytotoxicity in both the serum and peritoneal fluid was found in the fraction corresponding to a molecular weight of approximately 54,000-56,000 on hplc and the pi was found to be 4.9-5.1 by isoelectric focusing. these results are consistent with ...	1986	3722836
[studies on experimentally induced acute hepatic failure in mice: the lps-nonresponder mice].		1986	3723850
superiority of intralesional immunotherapy with corynebacterium parvum and allium sativum in control of murine transitional cell carcinoma.	immunotherapy with bacillus calmette-guerin (bcg), corynebacterium parvum (cp), keyhole limpet hemocyanin (klh) and an extract of allium sativum (as) was studied in a transitional cell carcinoma (mbt-2) in mice. comparison was made between intraperitoneal (ip) versus intralesional (il) administration of these agents. c3h/he mice were transplanted subcutaneously in the hind limb with 5 x 10(4) tumor cells. after transplantation, mice were randomized into groups to receive either ip or il treatmen ...	1986	3735559
natural killer cells in leukemogenesis.	in order to relate a reduced natural killer (nk) cell function to leukemogenesis, nk cells in the spleen and peritoneal exudate cells, with and without stimulation by corynebacterium parvum, were tested in mice of various strains after split dose irradiation and after leukemogenic treatment with butyl- and methylnitrosourea. the investigations included also mice submitted to non-leukemogenic irradiation (1 x 1.5 and 1 x 4.5 gy) and mice submitted to an additional treatment with hydrocortisone, w ...	1986	3736113
measurement of nadph oxidase activity in detergent lysates of human and mouse macrophage monolayers.	an assay to measure nadph oxidase activity in detergent lysates of macrophage monolayers is described. the addition of a reaction mixture containing appropriate concentrations of disrupting detergents, nadph as oxidase substrate and cytochrome c as electron acceptor, to macrophages monolayers permits the reliable detection of a superoxide dismutase-sensitive nadph-dependent cytochrome c reductive activity. this activity is strictly substrate dependent and nadh could not substitute for nadph. the ...	1986	3760584
inhibition of wound healing by corynebacterium parvum.	corynebacterium parvum (c. parvum), an immunostimulant, was examined for its effects on wound healing in mice. animals injected intraperitoneally with c. parvum, 1400 micrograms 48 hr prior to wounding had significantly decreased wound strength at 5, 7, 11, 14, and 21 days after wounding compared to saline-injected controls (p less than 0.05-p less than 0.001). mice injected with c. parvum at 48 or 2 hr before wounding, synchronous with wounding and 2 or 48 hr after wounding had significantly de ...	1986	3762128
propionibacterium acnes infection in neurosurgical patients. experience with high-dose penicillin therapy.	propionibacterium acnes is an underestimated but significant cause of cerebrospinal fluid (csf) infection after neurosurgical procedures and in the presence of prosthetic devices. the most effective therapy for such infections has not been defined. we report here our experience with the use of high-dose penicillin in the treatment of six patients with postoperative infection which was caused by p. acnes. all patients received 3-4 million units of penicillin by the intravenous route every four ho ...	1986	3762478
[immunotherapy with corynebacterium parvum in experimental influenza virus infections and mixed infections in mice].		1986	3763446
corynebacterium parvum followed by chemotherapy (actinomycin d and dtic) compared with chemotherapy alone for metastatic malignant melanoma.	seventy-nine patients with stage iii widely metastatic melanoma were prospectively randomised to a 'no treatment' control group who received on tumour progression dtic (250 mg/m2 i.v. daily x 5) and actinomycin d 1.5 mg/m2 on day 1. a total of six courses at 3-week intervals was given. chemotherapy was only given on progression of disease. the other group received initially corynebacterium parvum (2 mg/m2) every 3 weeks for a maximum of eight courses and then the same chemotherapy on evidence of ...	1986	3770045
immunobiological activities of synthetic lipid a analogs with low endotoxicity.	synthetic lipid a analogs, beta(1-6)glucosamine disaccharide 1,4'-bisphosphates, which possesses four tetradecanoyl groups at the 2- and 2'-amino, and 3- and 3'-hydroxyl groups (la-17-pp), and each two of the (r)-3-hydroxytetradecanoyl and tetradecanoyl groups at the 2- and 2'-amino and 3- and 3'-hydroxyl groups, respectively (la-18-pp), were far less endotoxic than synthetic (506, la-15-pp) and bacterial escherichia coli type lipid a's; neither compound showed any detectable lethal toxicity in ...	1986	3781622
isolation of intact chains of polyphosphate from "propionibacterium shermanii" grown on glucose or lactate.	a procedure is presented for the isolation of intact polyphosphate (poly p) from "propionibacterium shermanii." it is demonstrated, by including [32p]poly p during the extraction, that this procedure does not hydrolyze the poly p, and it is shown that two other widely used procedures do cause breakdown of the poly p. the procedure presented allows isolation of three fractions, short-chain poly p which is soluble in trichloroacetic acid, long-chain poly p which is soluble at neutral ph, and long- ...	1986	3782036
[intrahepatic cholestasis induced by heat-killed propionibacterium acnes in the tuberculin-sensitized guinea pig].		1986	3784062
[studies on an experimentally induced acute hepatic failure in mice: difference between the hepatotoxic factor and tumor necrosis factor].		1986	3784063
[use of propionibacterium granulosum kp 45 in the combined treatment of the inflammatory form of breast cancer].		1986	3786155
short-term failure of iud removal to alter bacterial flora in a patient with chronic anaerobic endometritis.	a patient with cytological and clinical evidence of iud-associated anaerobic endometritis was studied with quantitative and qualitative bacteriological techniques. with the exception of elimination of actinomyces israelii from the deep endocervical/endometrial culture, iud removal induced minimal qualitative changes in the bacterial flora of the endocervical/endometrial sample during the 35 days the patient was monitored. the interposition of menstruation did not significantly alter either the q ...	1986	3789249
biotin-dependent carboxylation catalyzed by transcarboxylase is a stepwise process.	to investigate the mechanism of the carboxylation of pyruvate to oxalacetate catalyzed by the enzyme transcarboxylase, we have measured the d(v/k) and 13(v/k) isotope effects. comparison of the double-reciprocal plots of the initial velocities with [1h3]pyruvate and with [2h3]pyruvate as substrate yields a deuterium isotope effect on vmax/km of 1.39 +/- 0.04. the 13c kinetic isotope effect on the carboxylation of pyruvate to oxalacetate has been measured by the competitive method and is 1.0227 + ...	1986	3790507
mutagenic activation of biliary metabolites of benzo(a)pyrene by beta-glucuronidase-positive bacteria in human faeces.	human faeces hydrolysed synthetic beta-d-glucuronides of both p-nitrophenol and phenolphthalein. the origin of this activity in faeces was localised in the bacterial pellet fraction after centrifugation. ninety-seven bacterial strains with beta-glucuronidase activity isolated from fresh human faeces were identified as species of bacteroides, peptostreptococcus, fusobacterium, propionibacterium, clostridium, eubacterium and bifidobacterium. they were classified into two groups according to their ...	1986	3795254