Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effects of a lipophilic environmental pollutant (ddt) on the phospholipid and fatty acid contents of bacillus stearothermophilus. | cultures of bacillus stearothermophilus grown in a complex medium containing 1 microm ddt, exhibited longer lag adapting periods, decreased specific growth rates, and lower growth yield as compared to control cultures. the membrane lipid composition from cells grown in the presence of the insecticide was significantly different from that of control cells. the effects of ddt (2,2-bis(p-chlorophenyl)-1, 1,1-trichloroethane) on growth and lipid composition of bacterial cells were also determined in ... | 1997 | 9419252 |
| [use of a microwave device for dental instrument sterilization: possibilities and limitations]. | the aim of the present study was the evaluation of the effectiveness as sterilizer of a modified version of a microwave device, which was previously tested by the same authors and found unsatisfactory. | 1997 | 9432563 |
| visualizing dna replication in a catalytically active bacillus dna polymerase crystal. | dna polymerases copy dna templates with remarkably high fidelity, checking for correct base-pair formation both at nucleotide insertion and at subsequent dna extension steps. despite extensive biochemical, genetic and structural studies, the mechanism by which nucleotides are correctly incorporated is not known. here we present high-resolution crystal structures of a thermostable bacterial (bacillus stearothermophilus) dna polymerase i large fragments with dna primer templates bound productively ... | 1998 | 9440698 |
| characterization of two terminal oxidases in bacillus brevis and efficiency of energy conservation of the respiratory chain. | the respiratory chain of bacillus brevis was analyzed. resting cells showed an h+/o ratio of 4.8-5.2 (5.01+/-0.26), when measured using an oxygen pulse method with endogenous substrates. this value is intermediate between those of bacillus subtilis (about 4), which predominantly expresses cytochrome aa3-type quinol oxidase, and bacillus stearothermophilus (about 6), which has quinol cytochrome c reductase plus caa3-type cytochrome c oxidase. measurement of respiration with various substrates, an ... | 1997 | 9443812 |
| protein and mg(2+)-induced conformational changes in the s15 binding site of 16 s ribosomal rna. | the bacillus stearothermophilus ribosomal protein s15 binds to the central domain of the 16 s rrna inducing a conformational change in a three-way helical junction. to understand the nature of this conformational change, extended-helical junctions were prepared to examine the effects of s15 or mg2+ binding on the relative helical orientation using native gel electrophoretic mobility and transient electric birefringence. the free junction is planar with approximately 120 degrees interhelical angl ... | 1998 | 9466923 |
| comparative study of the toxic actions of 2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane and 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene on the growth and respiratory activity of a microorganism used as a model. | a strain of bacillus stearothermophilus was used as a model for a comparative study of the toxic effect of 2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane and 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene. bacterial growth, the o2 consumption rate, and respiration-related enzymatic activities provided quantitative data in agreement with results reported for other systems. the use of this bacterium for screening for chemical toxicity is discussed. | 1997 | 9471966 |
| elimination of microorganisms from dental operatory compressed air. | compressed air is used to power high-speed handpieces, as well as to dry and clean surfaces in the oral cavity during patient treatment, in all dental operatories. the compressed air used in the dental operatories located in large institutions such as universities or hospitals is generally obtained from a central source, and is produced by continually running compressors. in operatories located in private practice settings, compressed air is obtained from small on-site air compressors, which may ... | 1998 | 9473876 |
| a bridging study between liquid chromatography and microbial inhibition assay methods for determining amoxicillin residues in catfish muscle. | a bridging study was conducted to establish the correlation between a liquid chromatographic (lc) method and a microbial inhibition (mi) method for analysis of amoxicillin residues in catfish muscle. the lc procedure involved precolumn derivatization with formaldehyde followed by lc separation with fluorescence detection. the mi procedure used bacillus stearothermophilus as the test organism and was validated in this study before the bridging investigation. the 2 methods were compared for determ ... | 1998 | 9477560 |
| engineering an enzyme to resist boiling. | in recent years, many efforts have been made to isolate enzymes from extremophilic organisms in the hope to unravel the structural basis for hyperstability and to obtain hyperstable biocatalysts. here we show how a moderately stable enzyme (a thermolysin-like protease from bacillus stearothermophilus, tlp-ste) can be made hyperstable by a limited number of mutations. the mutational strategy included replacing residues in tlp-ste by residues found at equivalent positions in naturally occurring, m ... | 1998 | 9482837 |
| an evaluation of sterilization of endodontic instruments in artificial sponges. | the ability to sterilize endodontic files inserted into synthetic sponges was tested. sponges were subjected to 5 cycles of either dry heat (driclave) or steam under pressure (autoclave) sterilization. sterilization was corroborated by microbiological tests. the sponges and files were pre-sterilized separately using steam under pressure. one hundred eighty files contaminated with bacillus stearothermophilus spores (experimental and positive control) and 60 noncontaminated files (negative control ... | 1998 | 9487869 |
| use of 3-d computer modelling and kinetic studies to analyse grapefruit pyrophosphate-dependent phosphofructokinase. | the glycolytic reaction of grapefruit ppi-dependent phosphofructokinase (pfp) depends on the presence of fru-2,6-p2 (ka = 6.7 nm). this molecule was further demonstrated in grapefruit juice sac cells. citrate, alpha-ketoglutarate and isocitrate competitively inhibited the binding of fru-2,6-p2 to pfp. the affinity for fru-6-p (km = 159 microm) and ppi (km = 33 microm) were not affected by the addition of these molecules. in the gluconeogenic reaction, the presence of fru-2,6-p2 did not affect th ... | 1997 | 9493054 |
| genes and enzymes of the acetyl cycle of arginine biosynthesis in the extreme thermophilic bacterium thermus thermophilus hb27. | an arginine biosynthetic gene cluster, argc-argj, of the extreme thermophilic bacterium thermus thermophilus hb27 was isolated by heterologous complementation of an escherichia coli acetylornithinase mutant. the recombinant plasmid (pthm1) conferred ornithine acetyltransferase activity to the e. coli host, implying that t. thermophilus uses the energetically more economic pathway for the deacetylation of acetylornithine. pthm1 was, however, unable to complement an e. coli arga mutant and no acet ... | 1998 | 9493385 |
| dose-response effects of raw potato starch on small-intestinal escape, large-bowel fermentation and gut transit time in the rat. | this study was designed to quantify starch digestion within the small and large bowels separately when raw potato starch (rps) was included at 0-240 g/kg in diets fed to growing male wistar rats. rps was incorporated in the diets at the expense of maize starch which was expected to be almost completely digested in the small bowel. the digestibility of the maize starch was 0.99 but only 0.28 of the rps was digested before the terminal ileum so that with increasing intakes of rps there was a progr ... | 1997 | 9497449 |
| cooperativity in bacillus stearothermophilus pyruvate kinase. | the enzyme pyruvate kinase (pk) from the moderate thermophile bacillus stearothermophilus has been used as a model system with which to investigate the homotropic and heterotropic cooperative interactions of the enzyme. cooperative ligand binding by the wild-type enzyme was measured using pre-steady-state and steady-state fluorescence spectroscopy, and steady-state kinetics. the results suggest that the cooperative structural changes induced by the substrate phosphoenolpyruvate (pep) are distinc ... | 1998 | 9500921 |
| gene cloning and characterization of thermostable lipase from bacillus stearothermophilus l1. | the gene coding for an extracellular lipase of bacillus stearothermophilus l1 was cloned in escherichia coli. sequence analysis showed an open reading frame of 1254 bp, which encodes a polypeptide of 417 amino acid residues. the polypeptide was composed of a signal sequence (29 amino acids) and a mature protein of 388 amino acids. an alanine replaces the first glycine in the conserved pentapeptide (gly-x-ser-x-gly) around the active site serine. the expressed lipase was purified by hydrophobic i ... | 1998 | 9501519 |
| cooperative folding of a protein mini domain: the peripheral subunit-binding domain of the pyruvate dehydrogenase multienzyme complex. | the peripheral subunit-binding domain from the dihydrolipoamide acetyltransferase (e2) component of the pyruvate dehydrogenase multienzyme complex from bacillus stearothermophilus is stably folded, despite its short sequence of only 43 amino acid residues. a 41 residue peptide derived from this domain, psbd41, undergoes a cooperative thermal unfolding transition with a tm of 54 degrees c. this three-helix protein is monomeric as judged by ultracentrifugation and concentration-dependent cd measur ... | 1998 | 9512717 |
| improving the thermostability of bacillus stearothermophilus neutral protease by introducing proline into the active site helix. | a proline residue was introduced into the n-terminus (ile140 and asp141), the middle (leu147) and the c-terminus (asp153) of the active site helix of bacillus stearothermophilus neutral protease for comparing the effects on the thermostability. introduction of a proline residue into the n-terminus at sites 140 and 141 increased the half-survival temperature (hst) by 7.5 and 2.8 degrees c, respectively, from 68.3 degrees c of the wild-type enzyme. a proline residue at leu147 decreased the hst by ... | 1997 | 9514114 |
| the s-layer proteins of two bacillus stearothermophilus wild-type strains are bound via their n-terminal region to a secondary cell wall polymer of identical chemical composition. | two bacillus stearothermophilus wild-type strains were investigated regarding a common recognition and binding mechanism between the s-layer protein and the underlying cell envelope layer. the s-layer protein from b. stearothermophilus pv72/p6 has a molecular weight of 130,000 and assembles into a hexagonally ordered lattice. the s-layer from b. stearothermophilus atcc 12980 shows oblique lattice symmetry and is composed of subunits with a molecular weight of 122,000. immunoblotting, peptide map ... | 1998 | 9515918 |
| sterilization of slide sheath anesthetic injection systems placed within sharps containers. | the purpose of this study was to evaluate the effect that two steam autoclaves and an unsaturated chemical vapor sterilizer had on killing bacterial endospores present on commercial spore strips or applied to sterile anesthetic injection systems placed within sharps containers. three types of sterilizers were used: a gravity steam autoclave, a high vacuum steam autoclave and an unsaturated chemical vapor sterilizer. the microbial challenge for the sterilizers were bacillus stearothermophilus spo ... | 1997 | 9517339 |
| room temperature sterilization of surfaces and fabrics with a one atmosphere uniform glow discharge plasma. | we report the results of an interdisciplinary collaboration formed to assess the sterilizing capabilities of the one atmosphere uniform glow discharge plasma (oaugdp). this newly-invented source of glow discharge plasma (the fourth state of matter) is capable of operating at atmospheric pressure in air and other gases, and of providing antimicrobial active species to surfaces and workpieces at room temperature as judged by viable plate counts. oaugdp exposures have reduced log numbers of bacteri ... | 1998 | 9523458 |
| lipid composition changes induced by tamoxifen in a bacterial model system. | a putative relationship between growth impairment of bacillus stearothermophilus by tamoxifen (tam) and tam-induced perturbation of the physical properties of bacterial membrane lipids has been observed. the supplementation of the growth medium with ca2+ (a membrane stabilizer) partially relieves growth inhibition by tam, allowing growth at tam concentrations that fully impair growth in the basal medium. b. stearothermophilus modifies the membrane lipid composition in response to the addition of ... | 1998 | 9528675 |
| studies on the heterologous expression of bstvi restriction endonuclease in escherichia coli. | bacterial restriction and modification systems must be regulated to avoid self-restriction. it is generally accepted that cognate dna methyltransferases normally protects both, the host's chromosome and extrachromosomal elements from the activity of their endonuclease counterparts. when the bstvirm genes from bacillus stearothermophilus v were subcloned into escherichia coli, several clones exhibiting a r+m- phenotype were originated. the present work was undertaken to analyze the possibility th ... | 1998 | 9530521 |
| bstb7si (r decreases ccggy), a thermostable isoschizomer of cfr10i, from a strain of bacillus stearothermophilus isolated from oil-contaminated soil in kuwait. | isolates of thermophilic bacteria from desert soil in kuwait, heavily contaminated with crude oil, have been screened for the presence of restriction endonuclease activity. one of the isolates (b7s), identified as bacillus stearothermophilus, showed a high level of restriction endonuclease activity when a cell-free extract was incubated with lambda bacteriophage dna at 65 degrees c. a type ii restriction endonuclease (bstb7si) has been partially purified from this isolate. bstb7si recognises the ... | 1998 | 9532739 |
| enzymatic synthesis of a new derivative of thiamin, o-alpha-glucosylthiamin. | a new transglucosylated derivative of thiamin could be synthesized by the actions of cyclomaltodextrin glucanotransferase from bacillus stearothermophilus and glucoamylase from rhizopus sp., in this order, on a mixture of dextrin and thiamin. the derivative was isolated in crystalline form and identified as 5'-o-(alpha-d-glucopyranosyl)thiamin by spectroscopy (fab-ms, uv, 1h-nmr, and 13c-nmr), thiochrome formation with k3 [fe(cn)6]-naoh reagent, and the hydrolysis products by alpha- and beta-glu ... | 1998 | 9532779 |
| a general method of domain closure is applied to phosphoglycerate kinase and the result compared with the crystal structure of a closed conformation of the enzyme. | the occurrence of large domain motions associated with the mechanism of action of many proteins is well established. we present a general method of predicting domain closure applicable to proteins containing domains separated by an apparent hinge. the method attempts to allow for natural directional bias within the closing protein by repeatedly applying a weak pulling force over a short distance between pairs of atoms chosen at random in the two domains in question. appropriate parameters govern ... | 1998 | 9533621 |
| a nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in lactococcus lactis. | lactococcus lactis, a gram-positive organism widely used in the food industry, is a potential candidate for the secretion of biologically useful proteins. we examined the secretion efficiency and capacity of l. lactis by using the staphylococcus aureus nuclease (nuc) as a heterologous model protein. when expressed in l. lactis from an efficient lactococcal promoter and its native signal peptide, only approximately 60% of total nuc was present in a secreted form at approximately 5 mg per liter. t ... | 1998 | 9537390 |
| identification of the structural similarity in the functionally related amidohydrolases acting on the cyclic amide ring. | the functionally related amidohydrolases, including d-hydantoinases, dihydropyrimidinases, allantoinases and dihydro-orotases, share a similar catalytic function of acting on the cyclic amide ring. we aligned 16 amidohydrolases by taking account of the conservative substitution and found a number of highly conserved regions and invariant amino acid residues. analyses of the secondary structure and hydropathy profile of the enzymes revealed a significant degree of similarity in the conserved regi ... | 1998 | 9537960 |
| guanidine hydrochloride-induced changes of the e2 inner core of the bacillus stearothermophilus pyruvate dehydrogenase complex. | the limited proteolysis of the bacillus stearothermophilus pyruvate dehydrogenase complex by v8 protease yields its core structure solely composed of lipoate acetyltransferase (e2) fragments. the changes in the core with guanidine hydrochloride (gdnhcl) were biphasic: below 0.8 m (first) and above 1.0 m (second) gdnhcl. the changes in the first phase were slight but significant: decreases in ellipticity and light scattering, and an increase in e2 activity. insignificant changes in the molecular ... | 1998 | 9538243 |
| evaluation of high-temperature and short-time sterilization of injection ampules by microwave heating. | the high-temperature and short-time sterilization by microwave heating with a continuous microwave sterilizer (mws) was evaluated. the evaluation were performed with respect to: [1] lethal effect against microorganisms corresponding to f-value, and [2] reliability of mws sterilization process. bacillus stearothermophilus atcc 7953 spores were used as the biological indicator and the heat-resistance of spores was evaluated with conventional heating method (121-129 degrees c). in mws sterilization ... | 1998 | 9542408 |
| comparative study of the catalytic domain of phosphorylating glyceraldehyde-3-phosphate dehydrogenases from bacteria and archaea via essential cysteine probes and site-directed mutagenesis. | phosphorylating glyceraldehyde-3-phosphate dehydrogenase (grap-dh) catalyzes the oxidative phosphorylation of d-glyceraldehyde-3-phosphate to form 1.3-diphosphoglycerate. the currently accepted mechanism involves an oxidoreduction step followed by a phosphorylation. two essential aminoacids, cys149 and his176 are involved in the chemical mechanism of bacterial and eukaryotic grap-dhs. roles have been assigned to the his176 as (a) a chemical activator for enhancing the reactivity of cys149, (b) a ... | 1998 | 9546660 |
| probing catalytic hinge bending motions in thermolysin-like proteases by glycine --> alanine mutations. | the active site of thermolysin-like proteases (tlps) is located at the bottom of a cleft between the n- and c-terminal domains. crystallographic studies have shown that the active-site cleft is more closed in ligand-binding tlps than in ligand-free tlps. accordingly, it has been proposed that tlps undergo a hinge-bending motion during catalysis resulting in "closure" and "opening" of the active-site cleft. two hinge regions have been proposed. one is located around a conserved glycine 78; the se ... | 1998 | 9548762 |
| a single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases. | thermostable thermolysin-like proteases (tlps), such as the tlp of bacillus stearothermophilus cu-21 (tlp-ste), bind calcium in one double (ca1,2) and two single (ca3, ca4) calcium binding sites. the single sites are absent in thermolabile tlps, suggesting that they are determinants of (variation in) tlp stability. mutations in the ca3 and ca4 sites of tlp-ste indeed reduced thermal stability, but only mutations in the ca3 site affected the calcium-dependence of stability. the predominant effect ... | 1998 | 9548763 |
| conformational variability of the n-terminal helix in the structure of ribosomal protein s15. | ribosomal protein s15 is a primary rna-binding protein that binds to the central domain of 16s rrna. s15 also regulates its own synthesis by binding to its own mrna. the binding sites for s15 on both mrna and rrna have been narrowed down to less than a hundred nucleotides each, making the protein an attractive candidate for the study of protein-rna interactions. | 1998 | 9562554 |
| s-layered aneurinibacillus and bacillus spp. are susceptible to the lytic action of pseudomonas aeruginosa membrane vesicles. | when s-layered strains of bacillus stearothermophilus and aneurinibacillus thermoaerophilus, possessing s-layers of different lattice type and lattice constant as well as s-(glyco)protein chemistry, and isogenic s-layerless variants were subjected to membrane vesicles (mvs) from p. aeruginosa during plaque assays on plates or cfu measurements on cell suspensions, all bacterial types lysed. electron microscopy of negative stains, thin sections, and immunogold-labelled mv preparations revealed tha ... | 1998 | 9573179 |
| molecular and enzymatic characterization of a maltogenic amylase that hydrolyzes and transglycosylates acarbose. | a gene encoding a maltogenic amylase of bacillus stearothermophilus et1 was cloned and expressed in escherichia coli. dna sequence analysis indicated that the gene could encode a 69,627-da protein containing 590 amino acids. the predicted amino acid sequence of the enzyme shared 47-70% identity with the sequences of maltogenic amylase from bacillus licheniformis, neopullulanase from b. stearothermophilus, and cyclodextrin hydrolase (cdase) 1-5 from an alkalophilic bacillus 1-5 strain. in additio ... | 1998 | 9578484 |
| escherichia coli ribosomal protein l3 stimulates the helicase activity of the bacillus stearothermophilus pcra helicase. | escherichia coli ribosomal protein l3 stimulates the in vitro helicase activity of bacillus stearothermophilus pcra helicase upon a variety of different substrates. l3 has no intrinsic helicase or atpase activity nor is it able to stimulate the atpase activity of pcra. gel mobility shift assays revealed that the affinity of pcra for a variety of different dna species (single-stranded, nicked and 3'-tailed) was enhanced in the presence of l3. we suggest that the stimulatory effect of l3 upon the ... | 1998 | 9580688 |
| the cbaab genes for bo3-type cytochrome c oxidase in bacillus stearothermophilus. | structural genes were cloned for cytochrome bo3-type cytochrome c oxidase recently isolated from a gram-positive thermophile bacillus stearothermophilus. sequencing and northern blotting analyses indicated that the two genes cbaa and cbab composed an operon encoding for subunits i and ii, respectively, and that the oxidase was soxb-type. they are the first genes for a soxb-type cytochrome c oxidase whose natural substrate is known. | 1998 | 9582433 |
| uch9, a new antitumor antibiotic produced by streptomyces: i. producing organism, fermentation, isolation and biological activities. | we developed a microbial prescreen using bacillus stearothermophilus nub3620 and bacteriophage tp-68 to detect potential antitumor compounds acting on dna or topoisomerases. during the course of screening microbial cultures for their antibacteriophage activities, we found that streptomyces sp. isolated from a soil sample collected in iwakuni city, yamaguchi prefecture, japan, produced a new antitumor antibiotic, uch9. uch9 was isolated from culture broth by a combination of etoac extraction and ... | 1998 | 9589060 |
| characterisation of bacillus stearothermophilus pcra helicase: evidence against an active rolling mechanism. | pcra from bacillus stearothermophilus is a dna helicase for which, despite the availability of a crystal structure, there is very little biochemical information. we show that the enzyme has a broad nucleotide specificity, even being able to hydrolyse ethenonucleotides, and is able to couple the hydrolysis to unwinding of dna substrates. in common with the escherichia coli helicases rep and uvrd, pcra is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5 ... | 1998 | 9592155 |
| cleaning of endodontic files, part i: the effect of bioburden on the sterilization of endodontic files. | ninety-two new endodontic files were randomly assigned to five groups with varying parameters of contamination, cleaning method, and sterilization (steam or chemical). files were instrumented in bovine teeth to accumulate debris and a known contaminant, bacillus stearothermophilus. positive controls produced growth on both t-soy agar plates and in t-soy broth. negative controls and experimental files (some with heavy debris) failed to produce growth. the results showed that there was no signific ... | 1997 | 9594742 |
| chemical modifications of bacillus subtilis tryptophanyl-trna synthetase. | a concerted conformational change in bacillus subtilis tryptophanyl-trna synthetase (trprs) was evident from previous fluorescence on the quenching of the single trp residue trp-92 in the 4ftrp-amp complexed enzyme. in this study, chemical modifications of the b. subtilis trprs were employed to further characterize this conformational change, with the single trp residue serving as a marker for monitoring the change. modifications of the enzyme by means of the trp-specific agent n-bromosuccinimid ... | 1997 | 9599659 |
| structure of the beta-galactosidase gene from thermus sp. strain t2: expression in escherichia coli and purification in a single step of an active fusion protein. | the nucleotide sequence of both the bgaa gene, coding for a thermostable beta-galactosidase of thermus sp. strain t2, and its flanking regions was determined. the deduced amino acid sequence of the enzyme predicts a polypeptide of 645 amino acids (mr, 73,595). comparative analysis of the open reading frames located in the flanking regions of the bgaa gene revealed that they might encode proteins involved in the transport and hydrolysis of sugars. the observed homology between the deduced amino a ... | 1998 | 9603833 |
| can a hydrophilic guidewire be resterilized? | the glidewire (microvasive, natick, ma) or terumo wire (terumo, japan) is constructed with a hydrophilic polymer surface that enables easier passage through narrowed lumens in the urinary tract. this study examined the effects of gas sterilization on glidewire surface structure, slipperiness, and ability to support bacterial growth. light microscopy at 100x and 400x and scanning electron microscopy at 100 to 1300x were used to compare the surface tips of five new 0.038-inch glidewires with those ... | 1998 | 9607451 |
| selection of biological indicator for validating microwave heating sterilization. | for the purpose of selecting an appropriate biological indicator for evaluation of the effects of microwave heating sterilization, we examined aerobic bacterial spores to determine whether microwaves have non-thermal sterilization effects. after microwave irradiation on dry bacterial spores (three species), none of the bacterial spores were killed. the survival rate of the spores after microwave irradiation of spore suspensions (twelve species) was compared with that after heating by a conventio ... | 1998 | 9610169 |
| the bacillus stearothermophilus argcjbd operon harbours a strong promoter as evaluated in escherichia coli cells. | we have shown that the b. stearothermophilus argcjbd genes form a single operon. in b. stearothermophilus, a specific repressor governs operon expression by binding to the argco operator site overlapping the parg promoter sequence (dion et al., 1997). therefore, the enzymatic and transcriptional analyses performed in this work did not reflect the potential strength of parg in the native host. for evaluation of the parg promoter strength, e. coli was used as a host since its own argr repressor do ... | 1998 | 9611259 |
| on the global architecture of initiation factor if3: a comparative study of the linker regions from the escherichia coli protein and the bacillus stearothermophilus protein. | initiation factor if3 is a protein involved in the initiation stage of protein synthesis. it consists of two global domains linked by a 20 residue long, solvent-exposed linker. recently, the structure of the n and c-terminal domains of the bacillus stearothermophilus protein have been solved by x-ray crystallography and the structure of the intact escherichia coli protein has been studied by nmr. these two studies have led to apparently contradictory models for the domain organization of if3. th ... | 1998 | 9614948 |
| degradation of fenitrothion by bacillus stearothermophilus adhering to silica. | b. stearothermophilus strain ag-49, when cultivated in mineral medium in the presence of silica (sa), adhered to sa. adhesion depended on age of culture, contact time and glucose concentration of the culture medium. mid-exponential phase culture (5 h) required minimum contact time (30 min) for maximum adhesion. 0.6% glucose concentration was optimum. quantitative variation in protein and saccharide extractable in sodium chloride and sodium dodecyl sulfate (sds) was observed. five % degradation o ... | 1998 | 9616053 |
| tyrosine quenching of tryptophan phosphorescence in glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. | tyrosine is known to quench the phosphorescence of free tryptophan derivatives in solution, but the interaction between tryptophan residues in proteins and neighboring tyrosine side chains has not yet been demonstrated. this report examines the potential role of y283 in quenching the phosphorescence emission of w310 of glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus by comparing the phosphorescence characteristics of the wild-type enzyme to that of appositely designed m ... | 1998 | 9635769 |
| crystal structure of ribosomal protein s8 from thermus thermophilus reveals a high degree of structural conservation of a specific rna binding site. | s8 is one of the core ribosomal proteins. it binds to 16 s rna with high affinity and independently of other ribosomal proteins. it also acts as a translational repressor in escherichia coli by binding to its own mrna. the structure of thermus thermophilus s8 has been determined by the method of multiple isomorphous replacement at 2.9 a resolution and refined to a crystallographic r-factor of 16.2% (rfree 27.5%). the two domains of the structure have an alpha/beta fold and are connected by a lon ... | 1998 | 9636713 |
| lysyl-trna synthetase from bacillus stearothermophilus. stopped-flow kinetic analysis of enzyme.lysyladenylate formation. | amino acid activation reaction of the lysyl-trna synthetase [l-lysine:trnalys ligase (amp forming); ec 6.1.1.6] from bacillus stearothermophilus was studied fluorometrically by the stopped-flow method. the addition of l-lysine to the enzyme solution caused quenching of the protein fluorescence and the subsequent addition of atp restored the quenched fluorescence [takita et al. (1996) j. biochem. 119, 680-689; takita et al. (1997) 121, 244-250]. in the stopped-flow analysis, however, the former f ... | 1998 | 9644244 |
| thermostable neutral protease resembling thermolysin derived from bacillus brevis mib001. | a microbe producing a protease with strong thermostability that was released extracellularly was isolated from soil. the isolate, mib001, grew at from 15 to 51 degrees c and ph 5.1-8.8 and was tentatively identified as a strain of bacillus brevis. rabbit antisera raised against a pure preparation of the protease did not cross-react with thermolysin or neutral metalloprotease from bacillus stearothermophilus kp1236. | 1998 | 9648239 |
| substrate-dependent enantioselectivity of a novel hydantoinase from arthrobacter aurescens dsm 3745: purification and characterization as new member of cyclic amidases. | a hydantoinase from arthrobacter aurescens dsm 3745 has been purified to homogeneity with a yield of 77% using a three-step purification procedure. the active enzyme is a tetramer consisting of four identical subunits, each with a molecular mass of 49670 da as determined by mass spectrometry. the n-terminal amino acid sequence of the enzyme indicates sequence identities to cyclic amidases involved in the nucleotide metabolism as the d-hydantoinase from agrobacterium radiobacter (53%), the d-sele ... | 1998 | 9650283 |
| the pyruvate dehydrogenase complex from thermophilic organisms: thermal stability and re-association from the enzyme components. | examples of pyruvate dehydrogenase complexes, and of its probable precursors, the pyruvate ferredoxin oxidoreductases, both isolated from thermophilic organisms, are described. the pyruvate ferredoxin oxidoreductases are mostly characterized from thermophilic archaea like sulfolobus solfataricus and pyrococcus furiosus. they retain their catalytic activity up to 60 and 90 degreesc, respectively. characteristic for the thermophilic nature is a biphasic temperature behavior, reflecting a more stab ... | 1998 | 9655930 |
| dimeric tyrosyl-trna synthetase from bacillus stearothermophilus unfolds through a monomeric intermediate. a quantitative analysis under equilibrium conditions. | tyrosyl-trna synthetase from bacillus stearothermophilus comprises an n-terminal domain (residues 1-319), which is dimeric and forms tyrosyladenylate, and a c-terminal domain (residues 320-419), which binds the anticodon arm of trnatyr. the n-terminal domain has the characteristic fold of the class i aminoacyl-trna synthetases. the unfolding of the n-terminal domain by urea at 25 degreesc under equilibrium conditions was monitored by its intensities of light emission at 330 and 350 nm, the ratio ... | 1998 | 9660761 |
| control of peptide deformylase activity by metal cations. | previous work indicated that peptide deformylase behaves as a metalloenzyme since the escherichia coli enzyme was shown to copurify with a zinc ion. the present study establishes that nickel:enzyme complexes can also be isolated provided that nickel salts were added in the buffers throughout the purification. similar results were obtained with the deformylases from thermus thermophilus and bacillus stearothermophilus. as a result of nickel binding, the catalytic efficiencies of peptide deformyla ... | 1998 | 9665853 |
| genetically engineered zinc-chelating adenylate kinase from escherichia coli with enhanced thermal stability. | in contrast with adenylate kinase from gram-negative bacteria, the enzyme from gram-positive organisms harbors a structural zn2+ bound to 3 or 4 cys residues in the structural motif cys-x2-cys-x16-cys-x2-cys/asp. site-directed mutagenesis of his126, ser129, asp146, and thr149 (corresponding to cys130, cys133, cys150, and cys153 in adenylate kinase from bacillus stearothermophilus) in escherichia coli adenylate kinase was undertaken for determining whether the presence of cys residues is the only ... | 1998 | 9668094 |
| obfuscation of allosteric structure-function relationships by enthalpy-entropy compensation. | the ph and temperature dependence of the allosteric properties of phosphofructokinase (pfk) from bacillus stearothermophilus have been studied from 5 to 9 and 6 to 40 degrees c, respectively. throughout this ph and temperature range the allosteric ligands mgadp and phospho(enol)pyruvate (pep) have no effect on kcat. the dissociation constants of the substrate, fructose 6-phosphate, and the allosteric ligands, as well as the absolute value of the coupling free energies between these ligands, all ... | 1998 | 9675201 |
| purification and properties of mangano-superoxide dismutase from a strain of alkaliphilic bacillus. | a mangano-superoxide dismutase (ec 1.15.1.1) was purified to homogeneity from a strain of alkaliphilic bacillus for the first time. the purified protein, with an isoelectric point of ph 4.5, had a molecular mass of approximately 50 kda and consisted of two identical subunits (25 kda). the n-terminal amino acid sequence was ala-tyr-lys-leu-pro-glu-leu-pro-tyr-ala-ala-asn-ala-leu-glu-pro-his-ile- asp-glu-ala. the optimum ph and temperature for the reaction were 7.5 and 35 degrees c, respectively. ... | 1997 | 9680305 |
| investigation of the structural basis for thermostability of dna-binding protein hu from bacillus stearothermophilus. | site-directed mutagenesis was used to identify amino acid residues essential for the thermostability of the dna-binding protein hu from the thermophile bacillus stearothermophilus (bsthu). two mutants, bsthu-a27s and bsthu-v42i, in which ala27 and val42 in bsthu were replaced by the corresponding amino acids ser27 and ile42, respectively, in the homologue from a mesophile b. subtilis (bsuhu), were less stable than the wild-type bsthu (63.9 degreesc), showing tm values of 58.4 degreesc and 60.1 d ... | 1998 | 9685334 |
| how well does the chemiclave sterilize handpieces? | using the food and drug administration's protocol for testing health care sterilizers, the author investigated the ability of chemical vapor and steam to sterilize handpieces. five internal sites of six high-speed handpiece models and four internal positions of one low-speed handpiece model were each inoculated with 10(6) bacillus stearothermophilus spores. half-cycle challenges were conducted with chemiclave models ec 5500 and 8000 (barnstead/thermolyne) and with two autoclaves, tuttnauer 2540m ... | 1998 | 9685763 |
| phosphopentomutase of bacillus stearothermophilus th6-2: the enzyme and its gene ppm. | phosphopentomutase catalyzes the transfer of an intramolecular phosphate on ribose or deoxyribose, and is involved in the salvage pathway of nucleoside synthesis. we identified a sequence 5'-upstream of the genes for the nucleoside phosphorylases of bacillus stearothermophilus as the phosphopentomutase (ppm) gene. the novel gene corresponded to an open reading frame of 1,179 nucleotides that is translated into a putative 393-amino acid protein with a molecular weight of 43,735. the gene product, ... | 1998 | 9692190 |
| stabilized enzymatic reagents for measuring glucose, creatine kinase and gamma-glutamyltransferase with thermostable enzymes from a thermophile, bacillus stearothermophilus. | stabilized enzymatic reagents for measuring some components in biological fluids have been successfully developed based on two kinds of thermostable enzymes derived from bacillus stearothermophilus with separation of the reagent into two complementary solutions. the thermostable glucokinase produced was applied to the measurement of glucose and creatine kinase activity, while the alanine dehydrogenase produced was used for the measurement of gamma-glutamyltransferase activity. the enzymatic reag ... | 1995 | 9696559 |
| influence of the secondary cell wall polymer on the reassembly, recrystallization, and stability properties of the s-layer protein from bacillus stearothermophilus pv72/p2. | the high-molecular-weight secondary cell wall polymer (scwp) from bacillus stearothermophilus pv72/p2 is mainly composed of n-acetylglucosamine (glcnac) and n-acetylmannosamine (mannac) and is involved in anchoring the s-layer protein via its n-terminal region to the rigid cell wall layer. in addition to this binding function, the scwp was found to inhibit the formation of self-assembly products during dialysis of the guanidine hydrochloride (ghcl)-extracted s-layer protein. the degree of assemb ... | 1998 | 9696762 |
| a c-terminal helicase domain of the human papillomavirus e1 protein binds e2 and the dna polymerase alpha-primase p68 subunit. | the human papillomavirus (hpv) e1 and e2 proteins bind cooperatively to the viral origin of replication (ori), forming an e1-e2-ori complex that is essential for initiation of dna replication. all other replication proteins, including dna polymerase alpha-primase (polalpha-primase), are derived from the host cell. we have carried out a detailed analysis of the interactions of hpv type 16 (hpv-16) e1 with e2, ori, and the four polalpha-primase subunits. deletion analysis showed that a c-terminal ... | 1998 | 9696837 |
| an investigation of the dynamics of ribosomal protein l9 using heteronuclear nmr relaxation measurements. | the dynamic properties of ribosomal protein l9 from bacillus stearothermophilus were investigated in solution using an analysis of nitrogen-15 longitudinal and transverse relaxation rates and amide nitrogen-proton nuclear overhauser effects. the relaxation rates of the amide nitrogen nuclei were found to be correlated with the angle between the amide nitrogen-proton bond vectors and the long axis of the protein. this directional dependence of the nuclear relaxation rates is consistent with the p ... | 1998 | 9698568 |
| effects of polyvalent cations on the folding of an rrna three-way junction and binding of ribosomal protein s15. | the bacillus stearothermophilus ribosomal protein s15 binds to a phylogenetically conserved three-way junction formed by the intersection of helices 20, 21, and 22 of eubacterial 16s ribosomal rna, inducing a large conformational change in the rna. like many rna structures, this three-way junction can also be folded by the addition of polyvalent cations such as magnesium, as demonstrated by comparing the mobilities of the wild-type and mutant junctions in the absence and presence of polyvalent c ... | 1998 | 9701289 |
| the crystal structure of ribosomal protein s4 reveals a two-domain molecule with an extensive rna-binding surface: one domain shows structural homology to the ets dna-binding motif. | we report the 1.7 a crystal structure of ribosomal protein s4 from bacillus stearothermophilus. to facilitate the crystallization, 41 apparently flexible residues at the n-terminus of the protein have been deleted (s4delta41). s4delta41 has two domains; domain 1 is completely alpha-helical and domain 2 comprises a five-stranded antiparallel beta-sheet with three alpha-helices packed on one side. domain 2 is an insertion within domain 1, and it shows significant structural homology to the ets dom ... | 1998 | 9707415 |
| the solution structure of ribosomal protein s4 delta41 reveals two subdomains and a positively charged surface that may interact with rna. | s4 is one of the first proteins to bind to 16s rna during assembly of the prokaryotic ribosome. residues 43-200 of s4 from bacillus stearothermophilus (s4 delta41) bind specifically to both 16s rrna and to a pseudoknot within the alpha operon mrna. as a first step toward understanding how s4 recognizes and organizes rna, we have solved the structure of s4 delta41 in solution by multidimensional heteronuclear nuclear magnetic resonance spectroscopy. the fold consists of two globular subdomains, o ... | 1998 | 9707416 |
| crystal structures of bacillus stearothermophilus adenylate kinase with bound ap5a, mg2+ ap5a, and mn2+ ap5a reveal an intermediate lid position and six coordinate octahedral geometry for bound mg2+ and mn2+. | crystal structures of bacillus stearothermophilus adenylate kinase with bound ap5a, mn2+ ap5a, and mg2+ ap5a have been determined by x-ray crystallography to resolutions of 1.6 a, 1.85 a, and 1.96 a, respectively. the protein's lid domain is partially open, being both rotated and translated away from bound ap5a. the flexibility of the lid domain in the ternary state and its ability to transfer force directly to the the active site is discussed in light of our proposed entropic mechanism for cata ... | 1998 | 9715904 |
| a parametric study of the destruction efficiency of bacillus spores in low pressure oxygen-based plasmas. | the destruction of bacillus spores in oxygen-based plasmas sustained in the millitorr pressure range has been studied as functions of various biological and plasma parameters, namely bacillus species, surface concentration of spores, treatment temperature, and gas composition. in an oxygen plasma, bacillus stearothermophilus appears less plasma-resistant than the other spores tested. oxygen, h2o2 and chiefly co2 plasmas are clearly shown to be much more efficient in destroying bacillus subtilis ... | 1998 | 9717311 |
| comparative evaluation of the sporicidal activity of new low-temperature sterilization technologies: ethylene oxide, 2 plasma sterilization systems, and liquid peracetic acid. | this study was undertaken to evaluate the efficacy of 4 new low-temperature sterilization technologies: ethylene oxide with hydrochlorofluorocarbons, a liquid peracetic acid immersion system (steris system 1 processor), and 2 plasma sterilization processes that use vaporized hydrogen peroxide (sterrad 100 and the sterrad 100s). the sterrad 100s system potentially improves sterilizer efficacy by using 2 cycles of a diffusion stage and a plasma stage per sterilization cycle. | 1998 | 9721391 |
| enzymatic properties of a novel liquefying alpha-amylase from an alkaliphilic bacillus isolate and entire nucleotide and amino acid sequences. | a novel liquefying alpha-amylase (lamy) was found in cultures of an alkaliphilic bacillus isolate, ksm-1378. the specific activity of purified lamy was approximately 5,000 u mg of protein-1, a value two- to fivefold greater between ph 5 and 10 than that of an industrial, thermostable bacillus licheniformis enzyme. the enzyme had a ph optimum of 8.0 to 8.5 and displayed maximum activity at 55 degreesc. the molecular mass deduced from sodium dodecyl sulfate-polyacrylamide gel electrophoresis was a ... | 1998 | 9726872 |
| cloning and characterization of plastid ribosomal protein s16 gene from potato (solanum tuberosum l. cv désirée). | the plastid ribosomal protein s16 (rps16) gene was cloned from potato (solanum tuberosum l. ssp. tuberosum cv desiree) by pcr amplification to obtain a new homologous recombination site of plastid transformation. the potato rps16 genomic clone was 1627 bp in size and the coding region was interrupted by an 859 bp intron. exon i was 40 bp, encoding 13 amino acids and exon ii was 227 bp, encoding a 76 amino acid polypeptide. the nucleotide sequence of the rps16 gene from the "désirée" potato share ... | 1998 | 9749535 |
| intersubunit structure within heterodimers of medium-chain prenyl diphosphate synthases. formation of a hybrid-type heptaprenyl diphosphate synthase. | among prenyltransferases that catalyze the sequential condensation of isopentenyl diphosphate with allylic diphosphate to produce prenyl diphosphates with various chain lengths and stereochemistries, medium-chain prenyl diphosphate synthases are exceptional in that they comprise two dissociable heteromeric protein components. these components exist without binding with each other under physiological conditions, and neither of them has any prenyltransferase activity by itself. in order to elucida ... | 1998 | 9756625 |
| preliminary x-ray crystallographic analysis of a novel maltogenic amylase from bacillus stearothermophilus et1. | a novel maltogenic amylase from bacillus stearothermophilus et1, which has a dual activity of alpha-1,4- and alpha-1,6-glycosidic bond cleavages and alpha-1,6-glycosidic bond formation, was crystallized by using the hanging-drop vapor-diffusion method. the best crystals were obtained by employing a high concentration of protein (56 mg ml-1) and a precipitant containing 22% glycerol, 1.6 m ammonium sulfate in 0.1 m tris-hcl (ph 8.5). native diffraction data to 2.66 a resolution have been obtained ... | 1998 | 9761914 |
| purification, crystallization and preliminary x-ray diffraction studies on the thermostable catechol 2,3-dioxygenase of bacillus stearothermophilus expressed in escherichia coli. | the thermostable catechol 2,3-dioxygenase of bacillus stearothermophilus has been crystallized. the crystal is probably in the space group i222 with unit-cell dimensions of a = 70.87, b = 74.60 and c = 133.69 a. a native data set has been collected with a completeness of 96% at 2.22 a resolution and an rmerge value of 0.091. | 1998 | 9761924 |
| identification of subunit interfaces of glycerol dehydrogenase from bacillus stearothermophilus. | 1998 | 9765996 | |
| studies on the folding and unfolding of glycerol dehydrogenase from bacillus stearothermophilus. | 1998 | 9765997 | |
| biochemical characterization of tellurite-reducing activities of bacillus stearothermophilus v. | bacillus stearothermophilus v is a naturally occurring gram-positive rod which exhibits resistance to potassium tellurite. crude extracts of this bacterium catalyse the nadh-dependent, protease-sensitive reduction of k2teo3 in vitro. two fractions which showed the ability to reduce potassium tellurite (h1 and h2) were obtained. fraction h1 behaved as a macroaggregate exhibiting a very high molecular mass that could not be estimated accurately. upon electrophoresis in polyacrylamide gels in the p ... | 1998 | 9766238 |
| structure and expression of elongation factor tu from bacillus stearothermophilus. | the tuf gene coding for elongation factor tu (ef-tu) of bacillus stearothermophilus was cloned and sequenced. this gene maps in the same context as the tufa gene of escherichia coli str operon. northern-blot analysis and primer extension experiments revealed that the transcription of the tuf gene is driven from two promoter regions. one of these is responsible for producing a 4.9-kb transcript containing all the genes of b. stearothermophilus str operon and the other, identified adjacent to the ... | 1998 | 9769211 |
| high-resolution electrospray ionization fourier transform mass spectrometry with infrared multiphoton dissociation of glucokinase from bacillus stearothermophilus. | glucokinase (gk, ec 2.7.1.2), a member of the enzyme family of hexokinases, has been shown to be linked to maturity-onset diabetes of the young type ii (mody-2). although nucleotide and amino acid sequence information are available for the human varieties, they are not known for the variety from bacillus stearothermophilus, which is often used in protein binding studies. here, a combination of electrospray fourier transform mass spectrometry (ftms) and infrared multiphoton dissociation (irmpd) i ... | 1998 | 9794087 |
| nonadditivity of mutational effects on the properties of catalase i and its application to efficient directed evolution. | catalase i of bacillus stearothermophilus has high catalatic and low peroxidatic activities. the mutant from the first random mutant population, d130n, which has higher peroxidatic and lower catalatic activities than those exhibited by the wild-type enzyme, was subjected to second random mutagenesis in observance of the change in reaction specificity. from the second mutant population, the mutant i108t/d130n/i222t was selected and examined. the reaction specificity of the purified enzymes reveal ... | 1998 | 9796828 |
| gene cloning, dna sequencing, and expression of thermostable beta-mannanase from bacillus stearothermophilus. | a dna genomic library constructed from bacillus stearothermophilus, a gram-positive, facultative thermophilic aerobe that secretes a thermostable beta-mannanase, was screened for mannan hydrolytic activity. recombinant beta-mannanase activity was detected on the basis of the clearing of halos around escherichia coli colonies grown on a dye-labelled substrate, remazol brilliant blue-locust bean gum. the nucleotide sequence of the mannanase gene, manf, corresponded to an open reading frame of 2,08 ... | 1998 | 9797302 |
| the crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation. | pyrimidine nucleoside phosphorylase (pynp) catalyzes the reversible phosphorolysis of pyrimidines in the nucleotide synthesis salvage pathway. in lower organisms (e.g. bacillus stearothermophilus) pynp accepts both thymidine and uridine, whereas in mammalian and other higher organisms it is specific for thymidine (designated thymidine phosphorylase, tp). pynp shares 40% sequence similarity (and presumably significant structural similarity) with human tp, which has been implicated as a growth fac ... | 1998 | 9817849 |
| mutational analysis of the conserved cationic residues of bacillus stearothermophilus 6-phosphoglucose isomerase. | the importance in catalysis of the conserved arginine (r207) and lysine residues (k144, k294, k356, and k425) of 6-phosphoglucose isomerase from bacillus stearothermophilus was assessed by site-directed mutagenesis and kinetic analysis. in general mutations had minor effects on the km for fructose 6-phosphate. more dramatic effects were seen on kcat. the r207a mutant had a five orders of magnitude decrease in kcat relative to the wild-type enzyme. there was a significant recovery, by three order ... | 1998 | 9826199 |
| global analysis of the thermal and chemical denaturation of the n-terminal domain of the ribosomal protein l9 in h2o and d2o. determination of the thermodynamic parameters, deltah(o), deltas(o), and deltac(o)p and evaluation of solvent isotope effects. | the stability of the n-terminal domain of the ribosomal protein l9, ntl9, from bacillus stearothermophilus has been monitored by circular dichroism at various temperatures and chemical denaturant concentrations in h2o and d2o. the basic thermodynamic parameters for the unfolding reaction, deltah(o), deltas(o), and deltac(o)p, were determined by global analysis of temperature and denaturant effects on stability. the data were well fit by a model that assumes stability varies linearly with denatur ... | 1998 | 9828007 |
| biological indicators for steam sterilization: characterization of a rapid biological indicator utilizing bacillus stearothermophilus spore-associated alpha-glucosidase enzyme. | the alpha-glucosidase enzyme was isolated from vegetative cells and spores of bacillus stearothermophilus, atcc 7953. spore-associated enzyme had a molecular weight of approximately 92,700, a temperature optimum of 60 degrees c, and a ph optimum of 7.0-7.5. the enzyme in crude aqueous spore extract was stable for 30 min up to a temperature of 65 degrees c, above which the enzyme was rapidly denatured. the optimal ph for stability of the enzyme was approximately 7.2. the alpha-glucosidase in crud ... | 1998 | 9830122 |
| transamination as a side-reaction catalyzed by alanine racemase of bacillus stearothermophilus. | the pyridoxal form of alanine racemase of bacillus stearothermophilus was converted to the pyridoxamine form by incubation with its natural substrate, d- or l-alanine, under acidic conditions: the enzyme loses its racemase activity concomitantly. the pyridoxamine form of the enzyme returned to the pyridoxal form by incubation with pyruvate at alkaline ph. thus, alanine racemase catalyzes transamination as a side function. in fact, the apo-form of the enzyme abstracted tritium from [4'-3h]pyridox ... | 1998 | 9832621 |
| amperometric phenol biosensor based on a thermostable phenol hydroxylase. | phenol hydroxylase (ec 1.14.13.7) was produced using bacillus stearothermophilus in a 5-1 batch fermentation leading to approximately 17 units after 6 h. the partially purified phenol hydroxylase was entrapped in a sol-gel matrix. the enzyme-loaded silica gel was attached to the sensitive top of a clark-type oxygen electrode and its application as a phenol biosensor was tested. there was linearity between the maximal rate of oxygen consumption and phenol concentration in the range between 2.5 an ... | 1998 | 9842702 |
| substituting selenocysteine for active site cysteine 149 of phosphorylating glyceraldehyde 3-phosphate dehydrogenase reveals a peroxidase activity. | replacing the essential cys-149 by a selenocysteine into the active site of phosphorylating glyceraldehyde 3-phosphate dehydrogenase (gapdh) from bacillus stearothermophilus leads to a selenogapdh that mimics a selenoperoxidase activity. saturation kinetics were observed with cumenyl and tert-butyl hydroperoxides, with a better catalytic efficiency for the aromatic compound. the enzymatic mechanism fits a sequential model where the formation of a ternary complex between the holoselenoenzyme, the ... | 1998 | 9845330 |
| bacillus stearothermophilus sporulation response to different composition media. | to evaluate the effectiveness of 11 commonly used ingredients to improve bacillus stearothermophilus atcc 7953 sporulation, with high spore yields in a short period of incubation, 32 composition media were set up by a fractional factorial 2iv11-6 design at two levels: d-glucose (0.018-0.25%), l-glutamic acid (0.040-0.10%), yeast extract (0.050-0.40%), peptone (0.30-0.50%), sodium chloride (0.001-1.0%), magnesium sulfate (0.001-0.20%), ammonium phosphate (0.010-0.035%), potassium phosphate monoba ... | 1998 | 9846067 |
| predictive model to describe the combined effect of ph and nacl on apparent heat resistance of bacillus stearothermophilus. | the combined effect of ph and nacl on the apparent thermal resistance of bacillus stearothermophilus atcc 12980 spores was studied. spores were heated at different temperatures (115-125 degrees c) in mushroom substrate, acidified using glucono-delta-lactone to different ph levels (from 5.75 to 6.7), which contained concentrations of nacl that ranged from 0.5 to 3% (w/v). the recovery medium was acidified to the same ph level and contained the same nacl concentration as the heating menstruum. a f ... | 1998 | 9849781 |
| identification of two binding domains, one for peptidoglycan and another for a secondary cell wall polymer, on the n-terminal part of the s-layer protein sbsb from bacillus stearothermophilus pv72/p2. | first studies on the structure-function relationship of the s-layer protein from b. stearothermophilus pv72/p2 revealed the coexistence of two binding domains on its n-terminal part, one for peptidoglycan and another for a secondary cell wall polymer (scwp). the peptidoglycan binding domain is located between amino acids 1 to 138 of the mature s-layer protein comprising a typical s-layer homologous domain. the scwp binding domain lies between amino acids 240 to 331 and possesses a high serine pl ... | 1998 | 9852032 |
| a six-domain structural model for escherichia coli translation initiation factor if2. characterisation of twelve surface epitopes. | the escherichia coli translation initiation factor if2 is a 97 kda protein which interacts with the initiator fmet-trnafmet, gtp and the ribosomal subunits during initiation of protein biosynthesis. for structural and functional investigations of the factor, we have raised and characterised monoclonal antibodies against e. coli if2. twelve epitopes have been localised at the surface of the protein molecule by three different methods: interactions of the monoclonal antibodies with nested deletion ... | 1998 | 9861457 |
| decreasing the stability and changing the substrate specificity of the bacillus stearothermophilus alcohol dehydrogenase by single amino acid replacements. | the gene encoding the alcohol dehydrogenase (adh-ht) from the thermophilic bacterium bacillus stearothermophilus lld-r strain has been overexpressed in escherichia coli and the corresponding recombinant protein purified to homogeneity. two putative structural determinants contributing to the higher stability of adh-ht had been identified by comparison with the less thermostable adh (adh-t) from the less thermophilic b. stearothermophilus nca 1503. in order to ascertain their role, mutations were ... | 1998 | 9862212 |
| [screening of thermophilic superoxide dismatase producing strain and some properties of the enzyme]. | a thermophilic sod producing strain was obtained from the bacteria preserved in our lab. its content of sod was 8774u/g fresh cells. the strain can tolerate 0.4% h2o2 and 70 degrees c, and it has outstanding culture characteristics. it was identified as bacillus stearothermophilus, called b.s 211-15. crude sod was extracted from b.s 211-15, the recovery of total activity was 69.5%, the specific activity was 1793u/mg protein. the enzyme showed fine heat stability, ph stability and good proteinase ... | 1997 | 9863206 |
| substrate specificity of thermostable farnesyl diphosphate synthase with alkyl group homologs of isopentenyl diphosphate. | 3-alkyl group homologs of isopentenyl diphosphate were examined for the reactivity as substrates of the thermostable farnesyl diphosphate (fpp) synthase of bacillus stearothermophilus. even 3-n-propyl- and 3-n-butyl-but-3-enyl diphosphates, which are hardly acceptable by animal fpp synthases, are accepted by this bacterial enzyme as substrates to react with dimethylallyl- and geranyl diphosphates, yielding 7-methyl-3-n-propylocta-2,6-dienyl- and 7,11-dimethyl-3-n-propyldodeca-2,6,10-trienyl diph ... | 1998 | 9873578 |
| expression of genes encoding the e2 and e3 components of the bacillus stearothermophilus pyruvate dehydrogenase complex and the stoichiometry of subunit interaction in assembly in vitro. | genes encoding the dihydrolipoyl acetyltransferase (e2) and dihydrolipoyl dehydrogenase (e3) components of the pyruvate dehydrogenase (pdh) multienzyme complex from bacillus stearothermophilus were overexpressed in escherichia coli. the e2 component was purified as a large soluble aggregate (molecular mass > 1 x 10(6) da) with the characteristic 532 symmetry of an icosahedral (60-mer) structure, and the e3 as a homodimer with a molecular mass of 110 kda. the recombinant e2 component in vitro was ... | 1998 | 9874216 |
| electron transfer kinetics of caa3 oxidase from bacillus stearothermophilus: a hypothesis for thermophilicity. | the o2 reaction and the reverse electron transfer of the thermophilic caa3 terminal oxidase of bacillus stearothermophilus have been studied by laser flash-photolysis. the results show that both reactions, although studied at a temperature of 20 degreesc, far from the optimal temperature of > 60 degreesc for caa3, follow a kinetic behavior essentially identical to that observed with the electrostatic complex between mammalian cyt c and cyt c oxidase. in the o2 reaction cyt a and cyt a3 are very ... | 1999 | 9876155 |
| functional domains of an nad+-dependent dna ligase. | limited proteolysis of the nad+-dependent dna ligase from bacillus stearothermophilus with thermolysin results in two fragments which were resistant to further proteolysis. these fragments were characterised by n-terminal protein sequencing and electrospray mass spectrometry. the larger, n-terminal fragment consists of the first 318 residues and the smaller, c-terminal fragment begins at residue 397 and runs to the c terminus. both fragments were over-expressed in escherichia coli and purified t ... | 1999 | 9878389 |