Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| influence of technical parameters on the in vitro motility of equine neutrophils in the presence of streptococcal culture supernatant. | to identify the influence of technical factors on the in vitro motility of equine neutrophils towards streptococcus culture supernatant in an under-agarose assay, we studied the changes in eight cell migration parameters. the distances the phagocytes travelled by directed, random and spontaneous migration increased with incubation time, cell concentration and the gelatin and serum contents of the migration plates. the contribution of chemotaxis to the phagocyte migrations, however, decreased sim ... | 1989 | 2515650 |
| antibacterial and plasmid curing activity of lomefloxacin in vitro. | the in vitro activity of lomefloxacin in comparison to other recently developed quinolones was evaluated by determination of mics for 89 test strains. organisms tested included clinical isolates of gram-positive and gram-negative bacteria susceptible or resistant to quinolones, resistant mutants of enterobacteriaceae, pseudomonas aeruginosa, staphylococcus aureus and enterococcus faecalis obtained in vitro, and the wild type strain escherichia coli k12 with its mutants na1a, nalb, nalc and nalb. ... | 1989 | 2515963 |
| construction of enterococcus faecalis pad1 miniplasmids: identification of a minimal pheromone response regulatory region and evaluation of a novel pheromone-dependent growth inhibition. | several pad1 miniplasmids were constructed that consisted of all or a portion of the pad1 ecori b fragment with pheromone-inducible "e region" lacz transcriptional fusions. miniplasmids containing the entire ecori b fragment (the "e miniplasmids") were found to regulate lacz expression normally, indicating that sufficient information is present on this fragment to regulate a pheromone response. these plasmids also encoded normal replication functions. the e miniplasmids were further reduced by d ... | 1989 | 2516332 |
| [comparison of the effectiveness of water disinfection by electrolysis and chlorination]. | 1989 | 2516492 | |
| nisin inhibits several gram-positive, mastitis-causing pathogens. | organisms known to cause bovine mastitis, enterococcus faecalis ssp. liquefaciens atcc 27959, staphylococcus aureus atcc 29740, streptococcus agalactiae atcc 27956, streptococcus equinus atcc 27960, streptococcus dysgalactiae atcc 27957, streptococcus uberis atcc 27958, and the neotype staphylococcus epidermidis atcc 14990 were examined for their susceptibility to the small peptide antibiotic, nisin. using a disc assay, minimum inhibitory concentrations of nisin ranged from 10 to 250 micrograms/ ... | 1989 | 2516858 |
| evolutionary relationships among bacterial carbamoyltransferases. | an immunological approach was used for the study of ornithine carbamoyltransferase (otcase) evolution in bacteria. antisera were prepared against the anabolic and catabolic otcases of pseudomonas aeruginosa and aeromonas formicans as well as against otcase and putrescine carbamoyltransferases from streptococcus faecalis; these antisera were then tested against the unpurified otcases, either anabolic or catabolic, of 34 bacterial strains. extensive cross-reactions were observed between the antise ... | 1989 | 2516870 |
| [pathogenic bacteria encountered in nutritional disorders]. | 1989 | 2517391 | |
| some properties of streptococcus faecalis subsp. liquefaciens isolated from cheese with special reference to production of bitterness. | streptococcus faecalis subsp. liquefaciens was examined for proteolysis and development of bitterness in sterile buffaloes' skim milk with and without some additives. cell population and the ph of milk were the most important factors in the breakdown of casein and development of bitterness. sodium chloride level, altering the final concentration of bacteria in milk, had a direct role in the production of bitter peptides. calcium ions up to 5 mm did not affect proteolysis whereas higher concentra ... | 1989 | 2517454 |
| [effect of selected bacteria on mycelial transformation of cells of candida albicans]. | influence of selected bacteria representing typical physiological flora of mucous membranes of man on transformation of candida albicans from yeast phase to mycelial phase was evaluated, e. coli, s. viridans and s. faecalis inhibited to different degree mycelial transformation of fungal cells. a degree of inhibition in the case of e. coli was proportional to the period of preliminary culture of strains in medium containing serum while streptococci inhibited mycelial transformation mainly after 4 ... | 1989 | 2518038 |
| [isolation and identification of the products of streptococcus faecalis inhibiting mycelial transformation of candida albicans]. | an attempt was made to isolate and identify streptococcus faecalis products responsible for the inhibition of mycelial transformation of candida albicans. five of streptococcal strains which 48 h broth culture supernatants run at 37 degrees c inhibited the most transformation of candida albicans from yeast phase to mycelial phage. the strains were cultivated for 48 h in tryptic soy broth at 37 degrees c, centrifuged and culture supernatants sterilised by means of filtration on millipore membrane ... | 1989 | 2518039 |
| the 1h nmr visibility of intracellular lactate in streptococcus faecalis. | 1h nmr studies of glycolysis in washed cell suspensions of streptococcus faecalis indicated that intracellular lactate is not 1h nmr visible. evidence for this was gained from time course studies of glycolysis at increasing concentrations of glucose. a close correlation existed between the relative increase in the lactate integral and the enzymatically determined extracellular lactate concentration [lo]. when ionophores which cause the collapse of the positive intracellular/extracellular lactate ... | 1989 | 2518155 |
| [urinary tract infections in primary care: predominant microbes and their antibiotic sensitivity]. | we have prospectively evaluated 227 episodes of community-acquired urinary tract infection (uti) from october 1st to december, 15th, 1987. the urine samples, adequately collected, were processed in the bacteriology laboratory of the albacete general hospital. e. coli was the most commonly isolated organism (62%), followed by proteus mirabilis (6.6%), streptococcus faecalis (6.6%), serratia marcescens (6.6%) and klebsiella pneumoniae (6.2%). as regards the sensitivity of e. coli to the most commo ... | 1989 | 2518913 |
| incidence of several pathogens and staphylococcal enterotoxins in kariesh cheese. | one hundred samples of kariesh cheese were randomly collected from fayoum markets and examined for the incidence of enterococci, coliform organisms, staphylococci, salmonella and detection of staphylococcal enterotoxins a, b, c, and d. the average total count was 16.4 x 10(7) c.f.u./gm. coliform bacteria were present in the all the examined, samples with an average of 18.9 x 10(3) c.f.u./gm. however, all samples were free from salmonella. faecal streptococci and staphylococci were also found in ... | 1989 | 2522177 |
| regulation of the cytoplasmic ph by a proton-translocating atpase in streptococcus faecalis (faecium). a computer simulation. | earlier work from this laboratory led to the proposal that the cytoplasmic ph of streptococcal cells is regulated solely by changes in the amount and activity of a proton-translocating atpase, f1f0 complex [kobayashi, h., suzuki, t. & unemoto, t. (1986) j. biol. chem. 261, 627-630]. we have now examined the proposal with the aid of computer simulation. we find that an increase in the amount of the h+-atpase is necessary for ph regulation and is sufficient to maintain a constant steady-state cyto ... | 1989 | 2522391 |
| comparative in vitro activity of the new oxazolidinones dup 721 and dup 105 against staphylococci and streptococci. | the in vitro activity of dup 721 and dup 105, two orally active members of the oxazolidinones, was compared with that of glycopeptides and ciprofloxacin against 185 gram-positive isolates. ninety percent of staphylococcus aureus isolates, including penicillin- and methicillin-resistant strains, were inhibited by dup 721 at 1 micrograms/ml and by dup 105 at 16 micrograms/ml; dup 721 inhibited 90% of coagulase-negative staphylococci tested at 1 micrograms/ml. the mic90 for streptococcus faecalis w ... | 1989 | 2523807 |
| leukopenia with neutropenia associated with teicoplanin therapy. | the first case report of leukopenia with neutropenia due to the new glycopeptide antibiotic teicoplanin is described. the side effect occurred in a 73-year-old man hospitalized because of subacute bacterial endocarditis caused by streptococcus faecalis. leukopenia with neutropenia (white blood cells 2000/mm3, neutrophils 46%) developed after 20 days of teicoplanin therapy. after stopping teicoplanin white blood cell and neutrophil counts reverted to normal, but dropped again on rechallenge. a re ... | 1989 | 2524129 |
| [sanitary and bacteriologic evaluation of black sea mussels]. | 1989 | 2526058 | |
| comparison of the agar dilution, tube dilution, and broth microdilution susceptibility tests for determination of teicoplanin mics. | the purpose of this study was to evaluate the national committee for clinical laboratory standards agar dilution, tube dilution, and broth microdilution susceptibility tests for the measurement of teicoplanin mics. the three standardized tests gave equivalent (within a twofold dilution) results with 98.8 to 99.0% of the 508 gram-positive clinical isolates tested, indicating that either method may be used for teicoplanin mic determination. | 1989 | 2526821 |
| the high-affinity k+-translocating atpase complex from bacillus acidocaldarius consists of three subunits. | cells of the thermoacidophilic bacterium bacillus acidocaldarius express a high-affinity k+-uptake system when grown at low external k+. a vanadate-sensitive, k+- and mg2+-stimulated atpase was partially purified from membranes of these cells by solubilization with a non-ionic detergent followed by ion-exchange chromatography of the extract. combinations of non-denaturing and denaturing electrophoretic separation methods revealed that the atpase complex consisted of three subunits with molecular ... | 1989 | 2527329 |
| evaluation of teicoplanin for treatment of endocarditis caused by gram-positive cocci in 20 patients. | teicoplanin, a new glycopeptide antibiotic similar to vancomycin, was evaluated for the treatment of bacterial endocarditis in an open multicenter study from may 1985 to august 1987. a total of 20 patients with positive blood culture endocarditis received teicoplanin once daily as a mean intravenous injection of 7.3 mg/kg of body weight (range, 4.8 to 10.6 mg/kg); in 17 patients, teicoplanin was combined with another antibiotic, usually an aminoglycoside. the mean duration of therapy was 28 days ... | 1989 | 2527483 |
| effects of clindamycin and gentamicin and other antimicrobial combinations against enterococci in an experimental model of intra-abdominal abscess. | a model was developed to permit direct assessment of antibiotic efficacy against enterococcus species in experimental intra-abdominal abscess. abscesses yielded enterococcus faecalis in pure culture. antimicrobials were delivered by precisely controlled continuous intravenous infusion. after five days of therapy, ampicillin alone or in combination with gentamicin reduced residual bacterial titers from 7.93 +/- 1.05 (untreated) to 3.51 +/- 1.07 and 3.52 +/- 0.81 log10 colony forming units per gra ... | 1989 | 2528215 |
| characterization of vancomycin resistance in enterococcus faecium and enterococcus faecalis. | vancomycin resistance in enterococcus faecium 180, a clinical isolate from england, was studied. resistance to vancomycin was transferable by conjugation to other enterococci. expression of resistance was inducible and coincided with the appearance of a new membrane protein. | 1989 | 2528940 |
| teicoplanin in the treatment of gram-positive-bacterial endocarditis. | intravenous teicoplanin has been used to treat 23 cases of gram-positive-bacterial endocarditis, usually with 3 to 7 mg/kg every 12 h on the first day, followed by 3 to 7 mg/kg every 24 h. for some cases (staphylococcal and enterococcal endocarditis), the dosage was 8 to 14.4 mg/kg per day and/or other antibiotics were given. the mean duration was 48.2 days (range, 23 to 130 days). of 23 patients, 21 (91.3%) had negative cultures or were cured. a total of 18 patients were treated with teicoplani ... | 1989 | 2529815 |
| sodium-translocating adenosine triphosphatase in streptococcus faecalis. | sodium-translocating atpase in the fermentative bacterium streptococcus faecalis exchanges sodium for potassium ions. sodium ions stimulate its activity, but k+ ions have no significant effect at present. although the molecular nature of the sodium atpase is not clear, the enzyme is distinct from other ion-motive atpases (e1e2 type and f1f0 type) as judged by its resistance to vanadate as well as dicyclohexylcarbodiimde. the sodium atpase is induced when cells are grown on media rich in sodium, ... | 1989 | 2531741 |
| rhizobium meliloti fixghi sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation. | we present genetic and structural analyses of a fix operon conserved among rhizobia, fixghi from rhizobium meliloti. the nucleotide sequence of the operon suggests it may contain a fourth gene, fixs. adjacent open reading frames of this operon showed an overlap between tga stop codons and atg start codons in the form of an atga motif suggestive of translational coupling. all four predicted gene products contained probable transmembrane sequences. fixg contained two cysteine clusters typical of i ... | 1989 | 2536685 |
| purification and substrate specificity of a strongly hydrophobic extracellular metalloendopeptidase ("gelatinase") from streptococcus faecalis (strain 0g1-10). | an extracellular zn-endopeptidase was purified to homogeneity from the culture filtrates of streptococcus faecalis (human oral strain 0g1-10) by a procedure that comprised concentration in an amicon hollow fiber system, ammonium sulfate precipitation, gel permeation chromatography, hydrophobic interaction chromatography (batch operation on phenyl-sepharose cl-4b), followed by fast protein liquid chromatography (fplc) on a phenyl-superose hr 5/5 column, and finally fplc on a superose 12 hr 10/30 ... | 1989 | 2536744 |
| effects of spermine and spermidine on the inorganic pyrophosphatase of streptococcus faecalis. interactions between polyamines and inorganic pyrophosphate. | we have shown a dual role for mg2+ in the hydrolysis of ppi catalysed by inorganic pyrophosphatase (ppase; ec 3.6.1.1) of streptococcus faecalis; mg2+ is necessary for the formation of the substrates, mg1ppi2- and mg2ppi0, and it also acts as an allosteric activator [lahti + jokinen (1985) biochemistry 24, 3526-3530]. no activity can be observed with s. faecalis ppase in the absence of bivalent cations, which indicates that free ppi cannot serve as a substrate for this enzyme. however, significa ... | 1989 | 2541687 |
| in vitro synergism between daptomycin and fosfomycin against enterococcus faecalis isolates with high-level gentamicin resistance. | daptomycin and fosfomycin are two agents which inhibit different steps in peptidoglycan synthesis. we studied the in vitro activities of these drugs, alone and in combination, by time-kill techniques against 21 clinical isolates of enterococcus (streptococcus) faecalis demonstrating high-level resistance to gentamicin. combinations of fosfomycin and daptomycin exhibited synergistic bactericidal activity (100-fold decrease in cfu per milliliter at 24 h compared with daptomycin alone) against all ... | 1989 | 2543282 |
| pathogenicity of enterococci in a rat model of fecal peritonitis. | the pathogenicity of enterococci in intraabdominal sepsis has not been clarified. therefore, fecal-type peritonitis was induced in rats by intraperitoneal injection of barium sulfate along with a bacterial inoculum consisting of escherichia coli, bacteroides fragilis, and clostridium perfringens with or without streptococcus faecalis. mortality at 19 d and characteristics of intraabdominal abscesses in survivors at 19 d were analyzed. the presence of s. faecalis in the original inoculum was sign ... | 1989 | 2543707 |
| [bactericidal activity of daptomycin and vancomycin alone or in combination with tobramycin, netilmicin or ampicillin against enterococcus]. | the in vitro bactericidal activity of daptomycin and vancomycin alone or in combination was studied against enterococci in a microtiter checkerboard assay and by kill-kinetic experiments. daptomycin was more active than vancomycin and was bactericidal. better fractional bactericidal concentration indices were observed with combinations of vancomycin with aminoglycosides, but in kill-kinetic studies, the combinations of 4 mics of daptomycin with 4 mg/l of tobramycin or netilmicin produced the mor ... | 1989 | 2543946 |
| reduced adherence to traumatized rat heart valves by a low-fibronectin-binding mutant of staphylococcus aureus. | isogenic strains of staphylococcus aureus, differing in fibronectin binding, were constructed for studies of the contribution of fibronectin binding to the in vivo pathogenesis of staphylococcal disease. mutagenesis of s. aureus 879r4s was accomplished by mating with enterococcus faecalis fa378 that carried the transposon tn918. four low-fibronectin-binding mutants were identified that bound 24- to 35-fold less fibronectin than the parent strain did. a spectinomycin-resistant strain, r4ssp, was ... | 1989 | 2545622 |
| bacteria and gallstone nucleation. | this preliminary study reports for the first time that there might be a possible association between bacteria and the aetiology of some cholesterol calculi. the gall-bladder biles from 225 cholecystectomy patients underwent bacteriological and microscopic study. cholesterol calculi from 13 patients (10.2%) were observed to be associated with gall-bladder bile profusely infected with at least one bacterial species that was shown to possess beta-glucuronidase activity, an enzyme that is thought to ... | 1989 | 2546527 |
| effect of rice fiber on human fecal microflora. | the effects of brown rice, containing fourfold as much dietary fiber as polished rice, on the human fecal microflora were determined. significantly increased numbers of bifidobacterium adolescentis and enterococcus faecalis were observed during the brown rice intake, whereas the total counts and the numbers of bacteroides, eubacterium aerofaciens, and escherichia coli during the intake were lower than those before and after the intake. lower numbers of clostridia and low incidences of clostridiu ... | 1989 | 2547140 |
| in vitro activity of sk&f 104662, a new glycopeptide antibiotic. | the in vitro activity of sk&f 104662, a new glycopeptide antibiotic, against gram-positive bacteria was evaluated. activity was comparable to those of teicoplanin and vancomycin against most organisms. sk&f 104662 inhibited diphtheroids at concentrations of less than or equal to 0.5 microgram/ml. addition of human serum to the test medium lowered the inhibitory activity of this glycopeptide against some organisms by as much as eightfold. | 1989 | 2548444 |
| quality control parameters and interpretive criteria for in vitro susceptibility tests with the macrolide azithromycin. collaborative antimicrobial susceptibility testing group. | quality control parameters for broth microdilution and disk diffusion susceptibility tests were defined and the interpretive criteria for disk diffusion tests reviewed. for interpretation of tests with 15 micrograms azithromycin disks, the following criteria are recommended: greater than or equal to 19 mm for the susceptible category (mic less than or equal to 2.0 micrograms/ml) and less than or equal to 15 mm for the resistant category (mic greater than or equal to 8.0 micrograms/ml). using the ... | 1989 | 2548865 |
| comparative in vitro activity of daptomycin (ly146032) and vancomycin against gram-positive cocci determined using a pharmacokinetic model. | the activity of daptomycin (ly146032) and vancomycin was compared for methicillin-sensitive and methicillin-resistant (mrsa) strains of staphylococcus aureus and for enterococcus faecalis, using a semi-automated model that allows examination of time-kill curves with diminishing drug concentrations, thus reflecting in vivo pharmacokinetics. exposure to daptomycin resulted in rapid killing of all strains of staphylococci and enterococcus faecalis tested. methicillin-sensitive staphylococcal strain ... | 1989 | 2550236 |
| scanning electronmicroscopy of staphylococcus aureus and enterococcus faecalis exposed to daptomycin. | the novel lipopeptide antibiotic, daptomycin, at a concentration of 8 mg/l, caused gross morphological changes in both a methicillin-sensitive and a methicillin-resistant strain of staphylococcus aureus and in a strain of enterococcus faecalis. the earliest (after 1 h) surface lesion observed was the appearance of boss-like processes randomly distributed on the cell surface. later, grossly deformed bacteria were seen and in two of the three bacteria prolonged exposure led to degeneration of the ... | 1989 | 2550648 |
| sex pheromones and plasmid transfer in enterococcus faecalis. | plasmid-free enterococcus faecalis excrete peptides (sex pheromones) which specifically induce a mating response in strains harboring certain conjugative plasmids. the response is characterized by the synthesis of a "fuzzy" surface material, visible by electron microscopy, which is believed to facilitate the aggregation of donors and recipients. transconjugants which receive a specific plasmid shut down the production of endogenous pheromone; however, they continue to produce pheromones specific ... | 1989 | 2550976 |
| nucleotide sequence of a novel kanamycin resistance gene, apha-7, from campylobacter jejuni and comparison to other kanamycin phosphotransferase genes. | a novel kanamycin phosphotransferase gene, apha-7, was cloned from a 14-kb plasmid obtained from a strain of campylobacter jejuni and the nucleotide sequence of the gene was determined. the presumed open reading frame of the apha-7 structural gene was 753 bp in length and encoded a protein of 251 amino acids with a calculated weight of 29,691 da. a 29-kda protein was demonstrated in escherichia coli maxicells containing the cloned apha-7 gene. a ribosomal binding site corresponding to 5 of 8 bas ... | 1989 | 2550983 |
| study by nmr of the mode of action of monensin on streptococcus faecalis de-energized and energized cells. | streptococcus faecalis was used as a bacterial model for studying the mode of action of monensin by nmr investigations. experiments were carried out in two states, characterized by several complementary methods: (i) the resting (de-energized) cell which was considered as an inert biological membrane, on which cationic transport induced by the ionophore alone can be investigated; (ii) the active (energized) cell where the ionophore-sensitive response of the living organism, particularly the catio ... | 1989 | 2551382 |
| in vitro postantibiotic effect of daptomycin (ly146032) against enterococcus faecalis and methicillin-susceptible and methicillin-resistant staphylococcus aureus strains. | the suppression of bacterial growth that persists after brief exposure to antimicrobial agents has been termed the postantibiotic effect (pae). this pharmacodynamic interaction varies for each microorganism-antimicrobial agent combination. daptomycin (ly146032) is a new lipopeptide antibiotic with activity against gram-positive organisms. we studied the in vitro bactericidal activities and paes of the following drugs: daptomycin compared with penicillin g and vancomycin, without and with gentami ... | 1989 | 2552901 |
| comparative in vitro activities of piperacillin-tazobactam and ticarcillin-clavulanate. | the in vitro activities of ticarcillin, piperacillin, clavulanic acid, tazobactam, ticarcillin-clavulanate, and piperacillin-tazobactam against 819 bacterial isolates were compared. the two beta-lactamase inhibitors, clavulanic acid and tazobactam, had little useful antibacterial activity but enhanced the activities of the penicillins against beta-lactamase-producing strains of haemophilus influenzae, branhamella catarrhalis, and methicillin-susceptible staphylococcus aureus; all strains were su ... | 1989 | 2552904 |
| genetic analysis of the conjugal transfer determinants encoded by the streptococcal broad-host-range plasmid pip501. | pip501 is a 30.2-kilobase (kb) broad-host-range conjugative streptococcal plasmid which encodes chloramphenicol and erythromycin resistance. a smaller conjugation-proficient derivative of pip501 has been constructed and designated pva1702. pva1702 is 25.2 kb, encodes only kanamycin resistance, and confers conjugative ability in an enterococcus faecalis mating system. the temperature-sensitive transposon delivery vectors ptv1ts and ptv32 carrying tn917 and tn917lac, respectively, were used to cre ... | 1989 | 2553667 |
| daptomycin (ly146032) for prevention and treatment of experimental aortic valve endocarditis in rabbits. | the efficacy of daptomycin (ly146032), a vancomycinlike lipopeptide antibiotic, was compared with that of antibiotics commonly in use for prevention and treatment of experimental aortic valve endocarditis in rabbits. strains of staphylococcus aureus. s. epidermidis, streptococcus sanguis, and enterococcus faecalis were used to establish endocarditis. a single 10-mg/kg dose of daptomycin and a single 25-mg/kg dose of vancomycin were both effective in prevention of endocarditis produced by strains ... | 1989 | 2554799 |
| in vitro susceptibility studies of vancomycin-resistant enterococcus faecalis. | vancomycin resistance exhibited by enterococcus faecalis isolates v583, v586, and v587 is described. the vancomycin mics ranged from 32 to 64 micrograms/ml. although resistant to vancomycin, the isolates were susceptible to teicoplanin (mic, less than or equal to 0.5 micrograms/ml). such a glycopeptide susceptibility profile has not been previously described for e. faecalis. time kill studies showed that vancomycin resistance adversely affected the synergistic activity that vancomycin and aminog ... | 1989 | 2554802 |
| ex vivo study of serum bactericidal titers and killing rates of daptomycin (ly146032) combined or not combined with amikacin compared with those of vancomycin. | twelve volunteers, in two groups of six, received daptomycin at a dose of 1 or 2 mg/kg. in addition, they received in a randomly allocated order amikacin (500 mg), daptomycin-amikacin, and vancomycin (500 mg). thirty-five clinical isolates, including staphylococcus aureus, s. epidermidis, corynebacterium sp. group jk, and enterococcus faecalis, were tested in vitro for the measure of the serum bactericidal titers and killing rates. the mean peak concentrations of daptomycin in serum 1 h after th ... | 1989 | 2556079 |
| introduction of transposon tn916 dna into haemophilus influenzae and haemophilus parainfluenzae. | enterococcus (streptococcus) faecalis transposon tn916 was introduced into haemophilus influenzae rd and haemophilus parainfluenzae by transformation and demonstrated to transpose efficiently. haemophilus transformants resistant to tetracycline were observed at a frequency of approximately 3 x 10(2) to 5 x 10(3)/micrograms of either pam120 (pgl101::tn916) or pam180 (pam81::tn916) plasmid dnas, which are incapable of autonomous replication in this host. restriction enzyme analysis and southern bl ... | 1989 | 2556369 |
| comparative study of visual inspections and microbiological sampling in premises manufacturing and selling high-risk foods. | the possible relationship between the results of a microbiological sampling programme and visual inspections carried out in local food-manufacturing premises was examined. using five main parameters - overall appearance, personal hygiene, risk of contamination, temperature control, and training and education - a visual inspection rating score was established for each of the premises. a variety of high-risk processed foods, and specimens from hands, wiping cloths and environmental swabs were exam ... | 1989 | 2558030 |
| the survival patterns of selected faecal bacteria in tropical fresh waters. | the survival of various faecal bacteria used as indicators of the faecal contamination of water supplies has been investigated in a tropical environment (sierra leone). isolates representing the thermotolerant coliform (ttc) and faecal streptococcus (fs) groups, clostridium perfringens and salmonella spp. were studied over a 48 h period of immersion in water from three different sources. survival patterns varied according to source type, but some general observations were made: a portion of the ... | 1989 | 2558031 |
| transfer of tn1545 and tn916 to clostridium acetobutylicum. | tn1545, a conjugative transposon originally discovered in streptococcus pneumoniae, has been transferred from enterococcus faecalis and bacillus subtilis to clostridium acetobutylicum ncib 8052. transfer between different strains of c. acetobutylicum has also been observed. insertion of tn1545 into the c. acetobutylicum chromosome occurred at multiple sites, as shown by southern hybridization. although ermam (erythromycin-resistance) was the most satisfactory marker for primary selection of tran ... | 1989 | 2560219 |
| [bacteriological, pharmacokinetic and clinical studies on cefpodoxime proxetil in the pediatric field]. | bacteriological, pharmacokinetic and clinical studies on cefpodoxime proxetil (cpdx-pr, cs-807), a new oral cephem antibiotic, were carried out in the field of pediatrics. the results obtained are summarized as follows. 1. antibacterial activities of r-3746 (na-salt of cefpodoxime (cpdx] against clinically isolated strains of streptococcus pneumoniae, streptococcus pyogenes, staphylococcus aureus, enterococcus faecalis, branhamella catarrhalis, escherichia coli, proteus mirabilis and haemophilus ... | 1989 | 2562789 |
| [clinical studies on cefpodoxime proxetil dry syrup in the field of pediatrics]. | cefpodoxime proxetil (cpdx-pr, cs-807) dry syrup was administered orally to 31 patients with various infections at daily dose levels between 5.4 and 10.9 mg/kg divided into three doses. 1. the subjects were 3 patients with urinary tract infections, 25 with tonsillitis and 1 patient each with bronchitis, pneumonia, and cervical lymphadenitis. clinical effects were excellent in 16 cases, good in 14, and fair in 1 (tonsillitis), with an overall efficacy rate of 96.8%. 2. organisms suspected as path ... | 1989 | 2562790 |
| dna probe for identification of streptococcus pneumoniae. | a total of 287 clinical isolates of streptococcus pneumoniae (pneumococcus) were tested for their ability to undergo autolysis when treated with sodium deoxycholate. the test was positive for all but one isolate, strain doc-1. this autolysis required the activity of an enzyme which is unique and characteristic of s. pneumoniae: a choline-dependent n-acetylmuramoyl-l-alanine amidase, the gene product of the lyta gene. we used lyta as a dna probe to test the distribution of the autolysin gene amon ... | 1989 | 2563386 |
| few-minutes tests for the identification of group a streptococci and enterococci with chromogenic substrates. | the usefulness of paper strip tests for rapid identification of streptococcus pyogenes and enterococci cultured on blood agar plates was investigated. the paper strips used contain dried chromogenic substrates for pyrrolidonyl peptidase (pyrase) and beta-glucosidase (beta-gluc). material from only a few colonies needed to be applied to the test strips. the reactions could be read after two min (pyrase) and 5-7 min (beta-gluc), respectively. results from testing 503 streptococcal strains were eva ... | 1989 | 2576626 |
| at2433-a1, at2433-a2, at2433-b1, and at2433-b2 novel antitumor antibiotic compounds produced by actinomadura melliaura. taxonomy, fermentation, isolation and biological properties. | compounds at2433-a1 (a1), at2433-a2 (a2), at2433-b1 (b1), and at2433-b2 (b2) were isolated from the cultured broth of actinomadura melliaura sp. nov. (scc 1655). structurally these materials are closely related to rebeccamycin (1), an indolocarbazole antitumor antibiotic. a1, a2, b1, and b2 were active against staphylococcus aureus a9537, streptococcus faecalis a20688, streptococcus faecium (atcc 9790), micrococcus lutea (atcc 9341), bacillus subtilis (atcc 6633). a1 and b1 were active against p ... | 1989 | 2584136 |
| comparison of two endometrial sampling devices. cotton-tipped swab and double-lumen catheter with a brush. | the potential for bacterial contamination during transvaginal recovery of endometrial samples in patients with postpartum endomyometritis spawned an investigation into various protective measures and techniques. the confirmation and determination of the extent of contamination was attempted in 120 postpartum women with the diagnosis of postpartum endometritis. each woman underwent endometrial sampling for bacterial culturing utilizing two devices--an unprotected, cotton-tipped swab and a protect ... | 1989 | 2585387 |
| intravenous/oral ciprofloxacin versus ceftazidime in the treatment of serious infections. | seventy-one adult patients with 72 infections were treated, by random selection, with intravenous/oral ciprofloxacin or intravenously administered ceftazidime. twenty-seven additional patients with 29 infections who were not appropriate for random assignment were treated in an open study with intravenously administered ciprofloxacin only; the latter infections were generally more serious or were caused by ceftazidime-resistant organisms. the most common doses were ciprofloxacin, 200 mg intraveno ... | 1989 | 2589361 |
| ciprofloxacin in patients with bacteremic infections. the spanish group for the study of ciprofloxacin. | the efficacy and safety of ciprofloxacin in the treatment of 68 episodes of bacteremia were studied. patients were treated intravenously (30 cases), orally (13 cases), or with sequential intravenous/oral therapy (25 cases). intravenous doses ranged from 200 to 400 mg per day and oral doses ranged from 1,000 to 1,500 mg per day. according to the criteria of mccabe and jackson, 39 cases had nonfatal and 29 had ultimately fatal underlying diseases. the clinical condition of patients at the start of ... | 1989 | 2589366 |
| comparative in vitro activity and beta-lactamase stability of fk482, a new oral cephalosporin. | fk482 is an oral aminothiazolyl hydroxyimino cephalosporin with a c-3 vinyl group. its activity was compared with those of cephalexin, cefuroxime, cefixime, and amoxicillin-clavulanate. fk482 inhibited 90% of staphylococcus aureus isolates at 1 micrograms/ml and 90% of streptococcus pyogenes, streptococcus agalactiae, and streptococcus pneumoniae isolates at less than or equal to 0.012 micrograms/ml, superior to cephalexin and cefuroxime and similar to cefixime. it did not inhibit oxacillin-resi ... | 1989 | 2589845 |
| infection of hemodialysis catheters: incidence and mechanisms. | fifty-three consecutive subclavian or jugular hemodialysis catheters inserted into 41 patients were prospectively studied over a period of 8 months in order to determine the incidence of infection and its mechanisms. the intravascular, intradermal and the y catheter segments as well as both connections were cultured using a quantitative technique for the intraluminal surface. in addition, the intravascular and intradermal portions of the catheter were cultured using a semiquantitative technique ... | 1989 | 2596535 |
| efficacy of clavulanate-potentiated antibiotics against bacteroides species and artificially associated cultures of aerobes and anaerobes. | clavulanic acid is known to potentiate the activity of amoxycillin and ticarcillin against beta-lactamase-producing bacteroides species. in order to assess the usefulness of the clavulanate antibiotics in mixed infections with aerobes and anaerobes, artificially associated cultures of clinically significant bacteroides species and facultative anaerobes (escherichia coli, klebsiella pneumoniae, haemophilus influenzae, enterococcus faecalis, staphylococcus spp. and streptococcus spp.) were tested. ... | 1989 | 2606820 |
| in vitro and in vivo antibacterial activities of kb-5246, a new tetracyclic quinolone. | the in vitro and in vivo antibacterial activities of kb-5246, a tetracyclic quinolone, were compared with those of ciprofloxacin, ofloxacin, and norfloxacin. kb-5246 demonstrated a broad antibacterial spectrum. the in vitro activity of kb-5246 against gram-negative bacteria was higher than that of ofloxacin or norfloxacin and was comparable to that of ciprofloxacin. kb-5246 demonstrated the greatest activity against gram-positive bacteria of the four agents tested. among streptococcus pyogenes s ... | 1989 | 2610501 |
| [clinical evaluation of cefmenoxime in infections of neonates]. | cefmenoxime (cmx) was evaluated for its absorption and excretion as well as for therapeutic effectiveness in neonates and premature infants. the following results were obtained. 1. serum concentrations of the drug were examined in 3 premature infants 1 to 11 days old upon intravenous administration of about 10 mg/kg body weight (1st group), in 2 premature infants 18 and 32 days old and 1 neonate 17 days old upon intravenous administration of about 20 mg/kg (2nd group), and in 1 neonate 15 days o ... | 1989 | 2614920 |
| [in vitro susceptibility to a new antimicrobial agent (imipenem) of pathogens isolated from inpatients at various centers]. | imipenem is a beta lactam antibiotic, a highly potent new carbapenem with broad antibacterial spectrum. to test the "in vitro" efficacy of this antimicrobial agent in pathogens more frequent in several medical centers in brazil, susceptibility testing with 10 mcg imipenem disks and, or corresponding mic were carried out with 1231 recent isolates of 41 different bacterial species, obtained mainly from hospitalized patients in 5 different medical centers of the cities of s. paulo, rio de janeiro a ... | 1989 | 2617012 |
| [clinical laboratory approach to estimating the effective administration dose of imipenem/cilastatin. evaluation of the disk susceptibility test and its interpretation system]. | in vitro activities of imipenem/cilastatin (ipm/cs) against 413 clinical isolates were studied through the evaluation of mics and the results of disk susceptibility tests. the mics were determined using the agar dilution method at an inoculum level of 10(6) cfu/ml. the mic80s of imipenem (ipm) against staphylococcus aureus and staphylococcus epidermidis were 25 and 1.56 micrograms/ml, respectively, showing a bimodal mic distribution. however, the distribution of mics against other bacteria studi ... | 1989 | 2621780 |
| [changes of bacterial flora on inbred mouse strains using experimental dental caries model]. | in the experimental dental caries model using inbred mouse strains, the bacterial flora of lower molars were investigated during the process of caries induction to examine the differences of bacterial flora between the caries susceptive mouse strains (balb/ca, c57bl/6n) and the caries resistant mouse strain (c3h/hen), as well as the relation between the bacterial flora and streptococcus faecalis that seemed to be the pathogen of caries on this caries model. the total cultivable bacterial number ... | 1989 | 2626795 |
| cockroaches as vectors of pathogenic bacteria. | one hundred and thirty two cockroaches of species blattella germanica--96 from hospital ward (test group) and 36 from residential areas (control group) were caught during nov. 1985 to nov. 1986. a variety of pathogenic and non-pathogenic bacteria were isolated from test and control group of insects. pseudomonas aeruginosa, staphylococcus aureus, streptococcus faecalis, and micrococci were isolated only from the test group of cockroaches. a high percentage (98.95 per cent) of test cockroaches wer ... | 1989 | 2638379 |
| polymicrobial bacteremia: clinical and microbiologic patterns. | between 1971 and 1987, 97 patients with polymicrobial bacteremia (pmb) were seen by a consulting infectious disease service. seventy-four had severe underlying illnesses, and infection was hospital acquired in 80. pmb resulted from intraabdominal, urinary tract, or soft tissue infection in 45 patients, but a wide range of sources were implicated in the rest. eleven patients had more than one source for the bacteremia, and, despite intensive diagnostic efforts, 24 had no identifiable source for a ... | 1989 | 2649955 |
| bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles. | the adhesion of salmonella typhimurium to the mineral particles quartz, albite, feldspar, and magnetite was shown to correlate with the hydrophobicity of the cell surface as measured by hydrophobic interaction chromatography. the same effects were also seen for seven other selected test strains, including streptococcus faecalis, streptococcus faecium, escherichia coli, citrobacter freundii, shigella sonnei, and shigella boydii. when the test strain of salmonella typhimurium, was repeatedly culti ... | 1989 | 2650618 |
| saliva-mediated aggregation of enterococcus faecalis transformed with a streptococcus sanguis gene encoding the ssp-5 surface antigen. | the interaction of a high-molecular-weight salivary glycoprotein (agglutinin) with streptococcus sanguis m5 leads to the formation of bacterial aggregates. we have previously shown that the ssp-5 surface antigen from s. sanguis m5 binds the salivary agglutinin and therefore may be involved in the aggregation process. here we report the transformation of a nonaggregating enterococcus faecalis strain with the ssp-5 gene and show that the protein is expressed on the cell surface and confers an aggr ... | 1989 | 2651309 |
| prophylactic co-trimoxazole versus norfloxacin in neutropenic children--perspective randomized study. | co-trimoxazole or norfloxacin were randomly administered to 44 granulocytopenic children with malignancies in order to prevent bacterial infections. although more patients in the co-trimoxazole group had febrile episodes (p less than 0.01), the mean of febrile days and the mean of days with systemic antibiotics did not differ significantly in the two groups. five patients in the co-trimoxazole group had a microbiologically documented infection (four with septicemia) due to escherichia coli (n = ... | 1989 | 2654019 |
| penetration of prophylactic antibiotics into peritoneal fluid. | this study evaluates the penetration of cephalosporins into the peritoneal fluid. forty-six patients scheduled for abdominal surgery were randomized into three groups. on call to the operating room each patient was given a single 1 g dose of cefazolin, cefuroxime, or ceftazidime. samples of the peritoneal fluid and blood were simultaneously obtained immediately after opening the peritoneal cavity. the mean serum cefazolin concentration was the highest. high peritoneal fluid levels of all three a ... | 1989 | 2658640 |
| [clinical experience of chemotherapy with cefmetazole for severe infections accompanying malignant hematological disorders]. | we made an attempt to treat with cefmetazole (cmz) 25 patients who developed severe infectious diseases while suffering with granulocytopenia associated with the treatment of malignant hematological disorders. 1. determination of bacteriological efficacy while 20 strains were isolated and identified from 15 patients, no significant bacteria were detected in 9 patients. isolates obtained were: 5 strains of enterococcus faecalis, 3 strains of haemophilus influenzae, 2 strains of staphylococcus epi ... | 1989 | 2664248 |
| [antibiotherapy in biliary surgery. current status]. | without antibiotherapy, biliary surgery is often followed by infectious complications, possibly serious, indeed life-threatening. biliary bacteria do are responsible of these complications; mainly e. coli, streptococcus faecalis (whose pathogenicity is disputed) and klebsiella. bacteroides fragilis and pseudomonas aeruginosa are restricted to special circumstances. it is often difficult to presee whether bile is infected: some risk factors were emerged by keighley but their specificity is not ex ... | 1989 | 2675657 |
| comparative in vitro activity of a new quinolone, am-1091. | the in vitro activity of a new quinolone, am-1091 [7-(3-amino-1-pyrrolidinyl)-8-chloro-1-cyclopropyl-6-fluoro-1,4-dihydro- 4-oxo- 3-quinoline carboxylic acid hydrochloride], was compared with those of ciprofloxacin, ofloxacin, beta-lactams, and gentamicin. am-1091 inhibited 90% of the isolates of the family enterobacteriaceae at less than or equal to 0.12 micrograms/ml. for many species am-1091 was 2-fold more active than ciprofloxacin and 2- to 32-fold more active than ofloxacin. it inhibited e ... | 1989 | 2675753 |
| [treatment of peritonitis under continuous ambulatory peritoneal dialysis using intraperitoneal ceftazidime]. | management of peritonitis related to chronic ambulatory peritoneal ceftazidime. in 15 patients under capd, 22 cases of bacterial peritonitis were observed with clinical manifestation in 14. the mean cell count in peritoneal dialysis fluid was 3 580/mm3 with 3 040/mm3 polymorphonuclear leukocytes. causative pathogens were: staphylococcus in 11 cases, streptococcus in 3, sarcines in 2, corynebacterium in 2, micrococcus varians in 1, gram negative in 3. first choice treatment was a intraperitoneal ... | 1989 | 2677930 |
| in vitro and in vivo activities of qa-241, a new tricyclic quinolone derivative. | the in vitro susceptibilities of 1,310 clinical isolates to qa-241, a novel tricyclic quinolone, were evaluated in comparison with susceptibilities to norfloxacin, ofloxacin, enoxacin, and ciprofloxacin. the mics of qa-241 for 90% of staphylococci, enterococcus faecalis isolates, and streptococcal species ranged from 1.56 to 6.25 micrograms/ml, and the activity of qa-241 was similar to those of norfloxacin and enoxacin but two to four times less potent than those of ofloxacin and ciprofloxacin. ... | 1989 | 2679369 |
| in vitro activity of cp-65,207, a new penem antimicrobial agent, in comparison with those of other agents. | cp-65,207 is a new parenteral penem antibiotic with a broad spectrum that includes gram-positive, gram-negative, and anaerobic microorganisms, with mics for 90% (mic90s) of the majority of 1,101 clinical pathogens tested being less than or equal to 1 microgram/ml. the compound was from 10- to 100-fold more active than cefoxitin and broad-spectrum cephalosporins against gram-positive bacteria and anaerobes. cp-65,207 was less active than imipenem for staphylococci, group a streptococci, and enter ... | 1989 | 2679370 |
| [the influence of cefpodoxime proxetil on the intestinal bacterial flora]. | the influence of cefpodoxime proxetil (cpdx-pr, cs-807), a new oral cephalosporin, on the intestinal bacterial flora was studied in tetra-contaminated mice and in pediatric patients. cpdx-pr dry syrup was administered at a dose of 10 mg/kg once a day for 5 consecutive days to mice contaminated with 4 different species of bacteria: escherichia coli, enterococcus faecalis, bacteroides fragilis and bifidobacterium breve. no notable changes were observed in fecal viable cell counts except that sligh ... | 1989 | 2681865 |
| sulbactam/ampicillin versus metronidazole/gentamicin in the treatment of post-cesarean section endometritis. | sixty-seven patients diagnosed with post-cesarean-section endometritis were studied in a prospective comparative randomized trial of sulbactam/ampicillin, a new beta-lactamase inhibitor drug combination, versus treatment with metronidazole/gentamicin. the success rate was 91% for each antibiotic regimen. mycoplasma spp. or ureaplasma spp. were isolated from all treatment failures. endometrial cultures revealed 2.3 aerobes as well as anaerobes per patient, with enterococcus faecalis, bacteroides ... | 1989 | 2686919 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from urinary tract infections (1986). i. susceptibility distribution]. | susceptibilities to various antibacterial and antibiotic agents of bacterial strains isolated from urinary tract infections at 8 hospitals in japan from july to october in 1986 are summarized as follows. 1. enterococcus faecalis was susceptible to sulfamethoxazole/trimethoprim (st) and imipenem (ipm) with mic90s of 0.78 and 1.56 microgram/ml. minocycline had the strongest activity against staphylococcus aureus; the mics for all strains tested were lower than 0.39 microgram/ml. the mic80s of dicl ... | 1989 | 2691714 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from urinary tract infections (1986). ii. background of patients]. | from july to october in 1986, hospital data on 717 isolates of causative microorganism from patients with urinary tract infections and background information on 562 patients were collected. the urinary tract infections were divided into 3 groups; simple infections and complicated infections with or without the use of indwelling catheter. we investigated such factors as the patients' sex, age, and drug treatments, and the isolates obtained from the infections. from 1981 to 1986, about 25% of the ... | 1989 | 2691715 |
| immune response in patients with intraabdominal infections treated with imipenem. swedish study group. | in the present investigation the humoral immune response against isolated microorganisms in patients treated with imipenem was studied. sixty-six patients (34 men and 32 women, 18-86 years of age) with suspicion of intraabdominal infections entered the study. five patients were excluded for various reasons and the remaining 61 patients were treated with imipenem/cilastatin 0.5-1.0 g t.i.d. for 5-29 days (median nine days). three serum samples were taken from each patient, the first sample at adm ... | 1989 | 2693357 |
| comparative in vitro antibacterial activity of the new carbapenem meropenem (sm-7338). | the in vitro antimicrobial activity of the new carbapenem meropenem (sm-7338) was determined by an agar dilution method in comparison with imipenem, ticarcillin/calvulanic acid, ceftazidime and the fourth-generation cephalosporin cefepime (bmy 28142). meropenem showed superior activity against enterobacteriaceae (mic90 less than or equal to 0.06 mg/l) and against non-fermentative gram-negative rods, with the exception of xanthomonas maltophilia. meropenem had excellent activity against beta-lact ... | 1989 | 2695328 |
| comparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen. | gram-negative and gram-positive bacteria were found to display different sensitivities to pure singlet oxygen generated outside of cells. killing curves for salmonella typhimurium and escherichia coli strains were indicative of multihit killing, whereas curves for sarcina lutea, staphylococcus aureus, streptococcus lactis, and streptococcus faecalis exhibited single-hit kinetics. the s. typhimurium deep rough strain ta1975, which lacks nearly all of the cell wall lipopolysaccharide coat and mani ... | 1989 | 2703469 |
| the enterococci: evidence of species-specific clinical and microbiologic heterogeneity. | investigations of the clinical role of enterococci have been limited largely to enterococcal bacteremia and endocarditis and have not distinguished between the various species of enterococci. to characterize the full spectrum of enterococcal disease and to determine whether clinically important differences exist among infections and/or instances of colonization (infections/colonizations) by the various enterococcal species, cases of enterococcal infection/colonization diagnosed at the dallas vet ... | 1989 | 2705463 |
| bacteriological findings after premature rupture of the membranes. | of 243 children born after premature rupture of the membranes (prom) 61 (26%) had the same bacteria in placental arterial blood, in ear swabs (taken deep from the external auditory canal) and in meconium. the predominant organisms were e. coli, bacteroides fragilis, streptococcus faecalis (enterococci) and streptococcus agalactiae (group b streptococci). the infection rate was only 10% if the membranes had ruptured within 24 h of the onset of labour and 30% if the interval was longer than 24 h. ... | 1989 | 2712599 |
| genetic basis of antibiotic resistance in aerococcus viridans. | resistance to at least one of the following antibiotics was found in eight wild-type strains of aerococcus viridans: erythromycin (six strains), tetracycline and minocycline (five strains), chloramphenicol (one strain), and high levels of streptomycin (one strain). none of the strains transferred any of their antibiotic resistance markers into streptococcal, enterococcal, or a. viridans recipients by conjugation. by dna-dna hybridization experiments, the ermb gene of transposon tn917, of enteroc ... | 1989 | 2729946 |
| in-vitro activity of pd 117558, a new quinolone against bacterial isolates from cancer patients. | the in-vitro activity of pd 117558, a new quinolone antimicrobial agent, was compared to that of four other quinolones, imipenem and ceftazidime against a variety of gram-positive and gram-negative isolates from cancer patients. pd 117558 was found to have a broad antimicrobial spectrum with excellent activity against all gram-positive isolates (including methicillin-resistant staphylococcus aureus, coagulase-negative staphylococci, streptococcus faecalis and group jk-corynebacteria). it was als ... | 1989 | 2732120 |
| in-vitro activity of the new penems fce 22101 and fce 24362 alone or in combination with aminoglycosides against streptococci isolated from patients with endocarditis. | seven strains of viridans streptococci isolated from patients with endocarditis were inhibited in vitro by 0.06-2 mg/l and 0.016-0.5 mg/l of the penems fce 22101 and fce 24362 respectively. the mbcs were the same or two-fold higher than the respective mic with three exceptions. one strain of streptococcus faecalis was only inhibited (8 mg/ml respectively 0.5 mg/l; mbc greater than 32 mg/l) and one strain of str. faecium was resistant (mic greater than or equal to 16 mg/l). when combined with gen ... | 1989 | 2732133 |
| in-vitro activity of the penem, fce 22101, and tentative interpretive criteria for disc susceptibility tests. | the in-vitro activities of two penems (fce 22101 and sch 34343) were compared to those of a carbapenem (imipenem), a monobactam (aztreonam) and three cephalosporins (cefoperazone, ceftazidime, and cefotaxime). fce 22101 displayed a broad spectrum of antibacterial activity against both gram-negative and gram-positive pathogens, including anaerobic bacteria. imipenem and aztreonam, however, were a little more potent against susceptible species. fce 22101 was bactericidal against 14 of 18 isolates ... | 1989 | 2732139 |
| association experiments with aerobic and anaerobic pathogens: a model of in-vitro susceptibility testing in mixed infections. activity of enoxacin, clindamycin, and metronidazole. | in infections of polymicrobial etiology, it seems mandatory to combine an antibiotic with marginal activity against anaerobes with an anti-anaerobic drug, e.g. metronidazole or clindamycin. we investigated the effect of associations of anaerobic and facultatively anaerobic pathogens on mbcs of enoxacin, clindamycin, metronidazole, and combinations of enoxacin plus clindamycin or metronidazole. single testing, and associations of bacteroides fragilis with escherichia coli or enterococcus faecalis ... | 1989 | 2737759 |
| studies on faecal streptococci in the river tigris. | six hundred water samples collected from the river tigris at mosul city were investigated for faecal streptococci. human faecal streptococci were predominant, and animal faecal streptococci were also detected. eight species and varieties were identified, viz streptococcus faecalis, atypical streptococcus faecalis, streptococcus bovis, streptococcus equinus, streptococcus faecalis var. liquefaciens, streptococcus faecalis var. zymogenes, streptococcus durans and streptococcus faecium. the inciden ... | 1989 | 2739584 |
| clinical evaluation of imipenem/cilastatin in the therapy of severe infections in high-risk patients. | a clinical trial was carried out to evaluate the effectiveness of treatment with imipenem/cilastatin in life-threatening infections in high-risk patients. thirty hospitalized patients (22 men and 8 women), aged 28 to 78 years, with impaired host defences and severe infections were treated with imipenem/cilastatin at a dosage ranging between 1 and 3 g daily i.v. at 8 or 12 h intervals. the average length of therapy was 8.61 +/- 3.14 days. the isolated organisms were as follows: escherichia coli [ ... | 1989 | 2743870 |
| [pharmacokinetic and bacteriological studies on sulbactam/ampicillin in the field of pediatrics]. | pharmacokinetic and bacteriological studies were carried out on sulbactam/ampicillin (sbt/abpc) in the field of pediatrics. the results obtained are summarized as follows: 1. a total of 248 clinical isolates were employed to determine minimum inhibitory concentrations (mic) of sbt/abpc against various bacterial species. sbt/abpc showed stronger antibacterial activity against gram-positive organisms than against gram-negative rods. 2. the peak serum level of abpc was about twice as high as that o ... | 1989 | 2746843 |
| [bacteriological, pharmacokinetic and clinical studies of sulbactam/ampicillin in the pediatric field]. | the usefulness of sulbactam/ampicillin (sbt/abpc) in the treatment of pediatric infections was evaluated. 1. twenty pediatric patients with infection were treated with sbt/abpc and an intravenous dosage of 27.8-47.4 mg/kg, 3 to 4 times a day. clinical efficacies in 18 patients excluding 2 patients of mycoplasma pneumonia (9 cases of pneumonia, 6 urinary tract infection, 1 tonsillitis, 1 maxillary sinusitis and 1 osteomyelitis) were judged to be excellent in 13 patients and good in 5. there was n ... | 1989 | 2746851 |
| [pharmacokinetic, bacteriological and clinical studies on imipenem/cilastatin sodium in neonates]. | pharmacokinetic, bacteriological and clinical studies on imipenem/cilastatin sodium (ipm/cs) were performed in neonates. the results obtained are summarized as follows. 1. plasma levels and urinary excretion of ipm and cs sodium were determined in 7 neonates with ages between 7 and 26 days (gestation periods were 37 to 41 weeks and birth weights were 2,410 to 3,890 g) upon 1 hour drip intravenous infusion of ipm/cs at 10 mg/10 mg/kg, or 20 mg/20 mg/kg. mean plasma concentrations of ipm reached t ... | 1989 | 2746858 |
| transcervical uterine cultures with a new endometrial suction curette: a comparison of three sampling methods in postpartum endometritis. | the potential for cervicovaginal contamination has precluded direct sampling of the endometrial cavity and has led to the development of multi-lumen protected catheter systems, among other techniques. the extent of this presumed contamination was examined using three different techniques in 55 postpartum women with the diagnosis of endometritis. each woman had the endometrium sampled with an unprotected cotton-tipped swab, a double-lumen catheter with brush, and a flexible endometrial suction cu ... | 1989 | 2748066 |
| activity of amine-stannous fluoride combination and chlorhexidine against some aerobic and anaerobic oral bacteria. | the in vitro susceptibility of 128 bacterial strains was tested to amine fluoride-stannous fluoride (amf + snf) and chlorhexidine (chx) solutions. actinobacillus actinomycetemcomitans, streptococcus mutans, and bacteroides intermedius were among the species investigated. the 50% and 90% minimal inhibitory concentrations (mics) were assessed by an agar dilution method. the mic ranges for a. actinomycetemcomitans were 2-32 micrograms/ml for chx and 0.25-64 micrograms/ml for amf + snf. the respecti ... | 1989 | 2762015 |
| [studies of lomefloxacin in biliary tract infections]. | lomefloxacin (ny-198), a new antimicrobial quinolone, was examined for its antimicrobial activities against clinical isolates and clinical efficacies to biliary tract infections. the following results were obtained. 1. the mics of ny-198 against escherichia coli (20 strains) and klebsiella pneumoniae (20 strains) were good and similar to those of ofloxacin (oflx) or norfloxacin (nflx). the mics of ny-198 against pseudomonas aeruginosa (20 strains) were inferior by 1 dilution factor to oflx or nf ... | 1989 | 2769934 |