Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| photodynamic effects of toluidine blue on human oral keratinocytes and fibroblasts and streptococcus sanguis evaluated in vitro. | some oral bacteria are susceptible to killing by red light after their sensitization with toluidine blue o (tbo). the photochemotherapy of periodontal disease in vivo would require a therapeutic window where bacteria could be killed without adjacent normal tissue damage. | 1996 | 8778520 |
| effects of sublethal exposure to an antiseptic mouthrinse on representative plaque bacteria. | although the mechanism responsible for the clinical antiplaque efficacy of oral antiseptics is generally considered to be primarily one of bactericidal activity, it has been suggested that oral antiseptics may have additional effects on bacteria exposed to sublethal levels. studies reported herein, investigated the effects of sublethal levels of an essential oil-containing antiseptic mouthrinse (listerine antiseptic, warner-lambert co., morris plains, nj) on selected activities of representative ... | 1996 | 8783049 |
| percutaneous drainage of septic glenohumoral arthritis. | 1996 | 8793441 | |
| experimental salivary pellicles formed on titanium surfaces mediate adhesion of streptococci. | the goal of this study was to characterize salivary components of titanium pellicles and to determine how experimental pellicles affect adhesion of several strains of streptococci to titanium surfaces. titanium experimental pellicles were formed by incubation of fresh human parotid or human submandibular-sublingual saliva on pure titanium beads. pellicle was recovered from the beads using sodium dodecyl sulfate buffer and was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis ... | 1996 | 8803339 |
| late bioprosthetic valve endocarditis caused by phialemonium aff. curvatum and streptococcus sanguis: a case report. | dual fungal and streptococcus sanguis endocarditis is reported in a 63-year-old woman 7 months after placement of a porcine aortic valve prosthesis. both micro-organisms were isolated by blood cultures, and the patient succumbed after a full course of antibacterial chemotherapy without having received antifungal chemotherapy. the best possible designation of the fungus was phialemonium aff. curvatum w. gams & w. b. cooke, as represented by cbs 331.93. at autopsy hyphae were revealed in the porci ... | 1996 | 8803803 |
| adsorption of saliva-coated and plain streptococcal cells to the surfaces of hydroxyapatite beads. | this study was conducted to observe the differences between the adsorption of streptococcus sanguis by saliva-coated and non-saliva-coated cells (plain cells) to the surfaces of saliva-coated, bovine serum albumin-coated or buffer-washed hydroxyapatite beads. saliva treatment of bacterial cells altered the manner of saturation due to changes of affinity and/or the maximal number of adsorption sites on the hydroxyapatite beads. this result indicates that saliva treatment of bacterial cells might ... | 1996 | 8809314 |
| the effect of oral commensal bacteria on candidal adhesion to human buccal epithelial cells in vitro. | the effect of streptococcus sanguis, s. salivarius, escherichia coli and porphyromonas gingivalis on the adhesion of candida albicans and c. krusei to human buccal epithelial cells (bec) was investigated with a modified membrane filter system. the filters (12 microns diameter pores) acted as a support for the bec which were pre-exposed to known concentrations of bacterial suspensions (for 45 min-1 h), and then re-incubated with standardised concentrations of yeast suspensions for various periods ... | 1996 | 8810944 |
| effect of local application of delmopinol hydrochloride on developing and early established supragingival plaque in humans. | the aim of the study was to investigate the effect of delmopinol hydrochloride on the development of dental plaque and on newly established plaque. in addition, the influence of this compound on the composition of the microbiota colonizing the gingival mucous membrane was studied. 14 healthy male volunteers took part. after a 3 week pre-experimental period of intense oral hygiene, the participants refrained from all oral hygiene for 14 days. the buccal surfaces of cuspids and bicuspids on one si ... | 1996 | 8811473 |
| clinical, microbiological and immunological profile of healthy, gingivitis and putative active periodontal subjects. | thirteen periodontally healthy subjects were monitored clinically for 6-12 months. clinical measurements at 6-weekly intervals included duplicate pd measurements, presence of plaque, redness, and bleeding on probing. baseline measurements consisted of 2 visits 1 wk apart. microbial samples were taken from 11 of the subjects who had completed at least 8 months of monitoring. levels of serum antibodies to 12 periodontal species were determined from 10 subjects. standard deviations of replicate pd ... | 1996 | 8814590 |
| liver abscess due to streptococcus sanguis. | 1996 | 8825006 | |
| evidence for the involvement of complement proteins in platelet aggregation by streptococcus sanguis nctc 7863. | we investigated the mechanisms of platelet aggregation by the type strain of streptococcus sanguis (nctc 7863). this species is one of the major aetiological agents of infective endocarditis. s. sanguis nctc 7863 caused aggregation of normal human platelets in vitro following a lag period that varied between donors (7-19 min). platelet aggregation was dependent on one or more plasma constituents and all the necessary factors gradually became bound to the bacterial surface during the lag period. ... | 1996 | 8826902 |
| bacterial adherence to the surface and isolated cell epithelium of the palatine tonsils. | bacterial adherence to the oropharyngeal epithelium is a significant factor in normal microecology, etiopathogenesis of diseases (tonsillitis, gingivitis) and possibly also induction of immune response. bacterial adhesion to human tonsillar epithelium of whole tonsils and swabs was studied by fluorescence, scanning, and transmission electron microscopy. the epithelial cell borders were well demarcated. on the apical surface of the cells there were irregular microridges. all forms of microscopy v ... | 1996 | 8831852 |
| inhibitory effects of funoran on the adherence and colonization of mutans streptococci. | funoran, a sulfated polysaccharide extracted from the seaweed gloiopeltis furcata strongly inhibited the absorption of mutans streptococci to saliva-coated hydroxyapatite (s-ha), but enhanced that of streptococcus sanguis atcc 10556 and streptococcus oralis atcc 10557. furthermore, funoran had a strong desorption activity against mutans streptococci preadsorbed to s-ha. in the presence of sucrose, the absorption of cells of streptococcus sobrinus b13, streptococcus mutans ingbritt, and s. mutans ... | 1996 | 8833135 |
| effect of chlorhexidine varnish on actinomyces naeslundii genospecies in plaque from dental fissures. | this study describes the effects of varnish containing 40% chlorhexidine diacetate on actinomyces naeslundii populations in plaque from human molar fissures. in each of 15 subjects two dental fissures with high levels of mutants streptococci were selected. the experimental treatment consisted of the single application of a small amount of chlorhexidine varnish onto the selected fissures. the varnish was removed 15 min after application. one month after varnish application a significant increase ... | 1996 | 8850582 |
| competitive binding of calcium, magnesium and zinc to streptococcus sanguis and purified s. sanguis cell walls. | calcium bound by oral bacteria may have important effects on plaque cohesion and mineral dynamics. equilibrium dialysis was used here to study the effect of magnesium and zinc competition on calcium-binding by streptococcus sanguis 7863. experiments were carried out using 45ca in 0.1-5.0 mmol/l ca2+ carrier. increasing [mg] and [zn] decreased the apparent ca-binding affinity, allowing calculation of the affinities for mg2+ and zn2+. dissociation constants were found to be 0.94 +/- 0.23, 1.32 +/- ... | 1996 | 8850586 |
| treatment of endocarditis with teicoplanin: a retrospective analysis of 104 cases. | infective endocarditis is an uncommon disease but retains a high mortality. glycopeptides are used for patients with resistant pathogens, those allergic to penicillins or for those outside the hospital. the once daily administration of teicoplanin and its low toxicity suggest that it would be suitable for use in the long courses required for endocarditis. however, the dosage and combinations to be used require further study. a retrospective review has been made of 104 episodes of endocarditis tr ... | 1996 | 8889725 |
| [behçet's disease and streptococcal antigens]. | many studies have been carried out concerning microbial infections and the occurrence of behçet's disease. recently, attention has been focused on the streptococci. the serum antibody titer and delayed type hypersensitivity of patients with behçet's disease against streptococcal antigens were significantly higher than those of controls. we also found that the oral flora of patients with behçet's disease was dominated by streptococcus sanguis, whose serotypes were different from controls. recent ... | 1996 | 8900580 |
| utilization of sialic acid by viridans streptococci. | the importance of viridans streptococci as agents of serious extra-oral diseases, including endocarditis, is now recognized. we have tested the hypothesis that the ability to utilize sialic acid as a nutrient source may play a role in the proliferation of these organisms. the type strains of the 15 presently recognized species of viridans streptococci and two clinical isolates-s. oralis (ar3), isolated from a patient with infective endocarditis, and s. intermedius (uns35), a brain abscess isolat ... | 1996 | 8906124 |
| platelet-streptococcal interactions in endocarditis. | infective endocarditis is characterized by the formation of septic masses of platelets on the surfaces of heart valves and is most commonly caused by viridans streptococci. streptococcal virulence in endocarditis involves factors that promote infectivity and pathogenicity. adhesins and exopolysaccharide (glycocalyx) contribute to infectivity. although many factors may contribute to pathogenicity, the platelet aggregation-associated protein (paap) of streptococcus sanguis contributes directly to ... | 1996 | 8909879 |
| effects of oral flora on platelets: possible consequences in cardiovascular disease. | during episodes of dental bacteremia, viridans group streptococci encounter platelets. among these microorganisms, certain streptococcus sanguis induce human and rabbit platelets to aggregate in vitro. in experimental rabbits, circulating streptococci induced platelets to aggregate, triggering the accumulation of platelets and fibrin into the heart valve vegetations of endocarditis. at necropsy, affected rabbit hearts showed ischemic areas. we therefore hypothesized that circulating s. sanguis m ... | 1996 | 8910832 |
| characterization of the streptococcus pneumoniae immunoglobulin a1 protease gene (iga) and its translation product. | bacterial immunoglobulin a1 (iga1) proteases constitute a very heterogenous group of extracellular endopeptidases which specifically cleave human iga1 in the hinge region. here we report that the iga1 protease gene, iga, of streptococcus pneumoniae is homologous to that of streptococcus sanguis. by using the s. sanguis iga gene as hybridization probe, the corresponding gene from a clinical isolate of s. pneumoniae was isolated in an escherichia coli lambda phage library. a lysate of e. coli infe ... | 1996 | 8926055 |
| identification, cloning, and sequencing of the immunoglobulin a1 protease gene of streptococcus pneumoniae. | the pneumococcus expresses a protease that hydrolyzes human immunoglobulin a1 (iga1). a gene for iga1 protease was identified from a plasmid library of pneumococcal dna because of the effect of its overexpression on the colony morphology of streptococcus pneumoniae. the deduced 1,964-amino-acid sequence is highly homologous to that of the iga1 protease from streptococcus sanguis. the similarity to the s. sanguis enzyme and the presence of a putative zinc-binding site suggest that the pneumococca ... | 1996 | 8926056 |
| disruption of the gene encoding penicillin-binding protein 2b (pbp2b) causes altered cell morphology and cease in exopolysaccharide production in streptococcus thermophilus sfi6. | genetic and biochemical analysis of exopolysaccharide (eps) production in lactic acid bacteria has been a growing field of interest in the food industry. we previously identified and characterized a gene cluster composed of 13 genes (epsa to epsm) responsible for eps production in streptococcus thermophilus sfi6. here we report one further gene, pbp2b, that is connected to eps production. mutants with a gene disruption in pbp2b were no longer able to produce eps, exhibited a reduced growth-rate, ... | 1996 | 8930919 |
| regional variation in root dentinal tubule infection by streptococcus gordonii. | the purpose of this study was to investigate the pattern of bacterial invasion of dentinal tubules at different regions in human roots. specimens were obtained from single-rooted teeth that had their root canals prepared in a standard manner. roots were then sectioned longitudinally through the canals and the resulting specimens chemically treated to remove the smear layers. specimens were immersed in a suspension of streptococcus gordonii for 3 weeks and then prepared for histological analysis. ... | 1996 | 8934987 |
| antibacterial activity of 10% carbamide peroxide bleaching agents. | the antibacterial activity of three commercial 10% carbamide peroxide bleaching agents (nite white, karisma, and opalescence) on streptococcus mutans, streptococcus mitis, streptococcus sanguis, lactobacillus casei, and lactobacillus acidophilus was examined. chlorhexidine (0.2%) was used as a positive control. test materials and control solution were inserted in wells punched on the surface of disk sensitivity agars seeded with bacteria. after incubation of 24 to 48 h, zones of inhibition were ... | 1996 | 8935060 |
| cloning of the foma gene, encoding the major outer membrane porin of fusobacterium nucleatum atcc10953. | the major outer membrane protein, foma, of the gram-negative human oral pathogen fusobacterium nucleatum functions as a porin and is assumed to act as a receptor protein in coaggregation with other oral pathogenic bacteria such as streptococcus sanguis and porphyromonas gingivalis. we describe here the cloning of foma from f. nucleatum in e. coli. using pgem3zf(+), three recombinant plasmids were carrying parts of the foma gene, but none of these contained regions upstream of the coding sequence ... | 1996 | 8938641 |
| lipoteichoic acid from viridans streptococci induces the production of tumor necrosis factor and nitric oxide by murine macrophages. | bacterial endotoxin or lipopolysaccharide is the major proinflammatory component of gram-negative bacteria, but the components of gram-positive bacteria that trigger the inflammatory cascade are poorly understood. lipoteichoic acid (lta) purified from 2 strains of viridans streptococci induced the accumulation of tumor necrosis factor (tnf) mrna and protein by the murine macrophage cell line raw 264.7 in a dose- and time-dependent manner. furthermore, in the presence of recombinant interferon-ga ... | 1996 | 8940232 |
| susceptibility of biofilms of streptococcus sanguis to chlorhexidine gluconate and cetylpyridinium chloride. | biofilms of streptococcus sanguis and planktonic cells of the organism were exposed to chlorhexidine gluconate and cetylpyridinium chloride, at concentrations used clinically, and survivors enumerated. s. sanguis exhibited a lower susceptibility to both antiseptics when it comprised a biofilm than when the organism was in the planktonic form. no viable bacteria were detectable after 5 min of exposure of planktonic cells to 0.2% (w/v) chlorhexidine gluconate or 0.05% (w/v) cetylpyridinium chlorid ... | 1996 | 8941774 |
| the establishment of a community of oral bacteria that controls the growth of candida albicans in a chemostat. | the purpose of this study was to establish and identify a community of oral bacteria that controls the growth of candida albicans in the chemostat. the chemostat was operated under glucose-limiting conditions at a dilution rate of 0.05 h-1 and inoculated with a yeast-free suspension of a tongue scraping. after a steady state had been reached, it was inoculated with c. albicans to establish the yeast and determine whether its growth could be contained. the steady-state community comprised the spe ... | 1996 | 8941776 |
| role of phagocytosis in activation of the coagulation system in streptococcus sanguis endocarditis. | the formation of vegetations consisting of fibrin, cellular elements, humoral factors, and bacteria is the central event in the pathogenesis of bacterial endocarditis. fibrin formation occurs on the vegetation, the coagulation system being activated locally via the expression of tissue factor (tf) on fibrin-adherent monocytes. this study was performed to assess the importance of phagocytosis of fibrin-adherent streptococcus sanguis in the stimulation of tf expression on fibrin-adherent monocytes ... | 1996 | 8945561 |
| recolonization with selected alpha-streptococci for prophylaxis of recurrent streptococcal pharyngotonsillitis--a randomized placebo-controlled multicentre study. | in a randomized, placebo-controlled, double-blind, multicentre study, 130 patients with recurrence of group a beta-hemolytic streptococci (gas) and clinical signs of pharyngotonsillitis were enrolled. the patients received antibiotic treatment for 10 days, followed by 10 days of alpha-streptococci-inhibitory to gas-or placebo spray treatment. patients taking antibiotic treatment for at least 9 days and using the spray for at least 5 days were included in the efficacy analysis. in addition, recur ... | 1996 | 8953674 |
| comparison of five methods for the determination of arginine hydrolysis by viridans streptococci. | seventy-eight clinical isolates and four control strains of viridans streptococci were tested in parallel for arginine hydrolysis by five different methods. these comprised two commercial systems, the api20 strep and vitek gpi card, two published methods, one based on ammonia production and one on alkalisation of møeller's decarboxylase medium, and a method based on alkalisation of a phenol-red broth medium dispensed into microtitration plates. the clinical isolates were speciated by their bioch ... | 1996 | 8958257 |
| importance of genotypic and phenotypic tolerance in the treatment of experimental endocarditis due to streptococcus gordonii. | genotypic and phenotypic tolerance was studied in penicillin treatment of experimental endocarditis due to nontolerant and tolerant streptococcus gordonii and to their backcross transformants. the organisms were matched for in vitro and in vivo growth rates. rats with aortic endocarditis were treated for 3 or 5 days, starting 12, 24, or 48 h after inoculation. when started at 12 h, during fast intravegetation growth, 3 days of treatment cured 80% of the nontolerant parent compared with <30% of t ... | 1997 | 8985198 |
| occurrence of lipoteichoic acid in oral streptococci. | the heterogeneous bacterial group known as oral streptococci was screened for the presence of cellular polyglycerolphosphate-containing lipoteichoic acid. this compound was detected in phenol extracts of lyophilized cells by an immunoassay in which polyglycerolphosphate-specific monoclonal antibody was used. polyglycerolphosphate-containing lipoteichoic acid occurred in all 86 strains of oral streptococci examined except the streptococcus mitis and streptococcus oralis strains. this confirms the ... | 1997 | 8995803 |
| shear bond strength, microleakage and antimicrobial properties of aelitebond. | to evaluate the shear bond strength (sbs) and microleakage (ml) to dentin as well as the antimicrobial action against five strains of oral bacteria of aelitebond single primer restorative system. | 1996 | 9002802 |
| antibacterial activity of silver ions implanted in sio2 filler on oral streptococci. | to evaluate the role of silver ions in composite resin dental materials, an in vivo investigation was conducted into the antibacterial effect of sio2 filler implanted with silver ions on oral streptococci. methods. sio2 filler samples (0.1g) were implanted with silver ions. the effect of the filler with silver ions (ag+ filler) was tested on oral streptococci bacteria. these bacterial strains had been isolated predominantly from composite resin surfaces. the organisms tested were anaerobically c ... | 1996 | 9002839 |
| electrophoresis of whole-cell soluble proteins of microorganisms isolated from bacteremias in endodontic therapy. | we have previously demonstrated that anaerobic bacteria are the microorganisms most frequently isolated from blood following endodontic therapy of teeth with apical periodontitis. phenotypic characterisation of the isolates suggested that the bacteria in the blood originated from the root canal. the present experiment using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was carried out in an effort to verify these findings, and to further study the microorganisms involved i ... | 1996 | 9021323 |
| [pyogenic hepatic abscess by streptococcus sanguis i]. | 1996 | 9053019 | |
| bacterial adherence to guided tissue regeneration barrier membranes exposed to the oral environment. | microbial colonization of barrier materials used in guided tissue regeneration (gtr) is known to adversely affect treatment outcomes. the purpose of this study was to compare the rate at which 11 commonly-occurring oral bacteria species colonize three different barrier materials (collagen, expanded polytetrafluoroethylene, and polylactic acid). the study group consisted of 10 systemically healthy individuals with no history of periodontal disease and absence of antimicrobial therapy within the p ... | 1997 | 9058336 |
| a numerical taxonomic study of streptococcus sanguis, s. mitis and similar organisms using conventional tests and pyrolysis mass spectrometry. | numerical taxonomic methods were applied to the results of pyrolysis mass spectrometry (py-ms) and conventional test reaction patterns (ctrps) obtained with 46 strains of oral streptococci identified as representative of six species. of these: streptococcus gordonii strains formed a single homogeneous well-differentiated cluster both in the py-ms and ctrp analyses; s. oralis strains were more diverse, forming 2 ctrp and 3 py-ms clusters; s. crista, s. parasanguis and s. sanguis formed clear, but ... | 1997 | 9060152 |
| external quality assessment of the serum bactericidal test: results of a methodology/interpretation questionnaire. | two hundred microbiology laboratories in the uk took part in two separate experimental external quality assessment distributions related to the serum bactericidal test (sbt). in the first, staphylococcus aureus nctc 6571 (vancomycin mic 1 mg/l), was tested against a human serum containing vancomycin 38 mg/l plus gentamicin 0.5 mg/l. in the second, streptococcus oralis paj 112/4183 (penicillin mbc < or = 0.03 mg/l) and streptococcus sanguis paj 107/4184 (penicillin mbc = 128 mg/l) were tested aga ... | 1997 | 9069554 |
| [serologic study in patients with streptococcal infective endocarditis]. | infective endocarditis is a systemic disease in which there are a continuously antigenic stimulation of immunologic system. streptococcus is still the most frequent cause of infective endocarditis. | 1996 | 9082078 |
| initial ph as a determining factor of glucose consumption and lactic and acetic acid production in oral streptococci. | lactic and acetic acid production was evaluated from six strains of oral streptococci, viz streptococcus gordonii, streptococcus mutans, streptococcus oralis, streptococcus salivarius, streptococcus sanguis and streptococcus sobrinus cultured in the presence of 1, 5, 10 mm glucose and without glucose, at initial ph values of 5, 5.5, 6 and 7. s. sobrinus and s. salivarius caused the greatest decreases in ph. at ph values of 5 and 5.5, lactic acid and acetic acid production in the species tested w ... | 1996 | 9082145 |
| [aortic valve perforation]. | 1996 | 9117668 | |
| effect of gentamicin dosing interval on efficacy of penicillin or ceftriaxone treatment of experimental endocarditis due to penicillin-susceptible, ceftriaxone-tolerant viridans group streptococci. | the efficacy of ceftriaxone or penicillin alone or combined with gentamicin at different dosing intervals was evaluated in experimental endocarditis due to a penicillin-susceptible, ceftriaxone-tolerant strain of streptococcus sanguis i. the difference between monotherapy with ceftriaxone and procaine penicillin approached statistical significance (p = 0.052). ceftriaxone combined with gentamicin administered as a single daily dose was less effective than was procaine penicillin combined with ge ... | 1996 | 9124865 |
| [atypical lung abscess occurring in an elderly female suffering from diabetes mellitus--a case report]. | we report an atypical case of lung abscess occurring in a 73-year-old female who suffered from diabetes for more than 20 years. in 1993 she had a total gasterectomy for gastric cancer. in 1995 she was admitted to our hospital complaining of a mild cough and a small amount of sputa. a ct scan of the chest revealed a huge abscess in the left lung. streptococcus sanguis was cultured from the intrathoracic fluid. it is possible that the severe lung abscess in spite of the few symptoms occurs in the ... | 1997 | 9128012 |
| saliva mediated adherence, aggregation and prevalence in dental plaque of streptococcus mutans, streptococcus sanguis and actinomyces spp, in young and elderly humans. | salivary components in the pellicle mediate bacterial adherence to the tooth. such components may also aggregate bacteria in saliva and prevent them becoming established in dental plaque. in the present study, the adherence and aggregation of streptococcus mutans strain ingbritt, s. sanguis strain 10556 and actinomyces viscosus-strain 19246 mediated by parotid and whole saliva from groups of young and elderly people were examined. significant differences were found between test strains, salivary ... | 1996 | 9134102 |
| comya, a gene similar to comga of bacillus subtilis, is essential for competence-factor-dependent dna transformation in streptococcus gordonii. | tn4001 mutagenesis identified a new competence gene in streptococcus gordonii challis designated comya. a comya mutant was completely deficient in transformation and exhibited decreased levels of dna binding and hydrolysis. the deduced 319-amino-acid comya protein exhibited 57% similarity and 33% identity to the comga transporter protein of bacillus subtilis and contained the walker a-box motif conserved in atp-binding proteins as well as aspartic acid boxes asp-1 and asp-2 present in some compo ... | 1997 | 9150204 |
| myeloperoxidase isoform activities released by human neutrophils in response to dental and periodontal bacteria. | myeloperoxidase is an enzyme released by polymorphonuclear leukocytes which has been used, experimentally, as an indicator of periodontal disease activity when measured in gingival crevicular fluid. there are three myeloperoxidase isoforms: mpo i, mpo ii and mpo iii. we examined the activities of myeloperoxidase isoforms released by neutrophils in response to serum-opsonized actinobacillus actinomycetemcomitans, haemophilus aphrophilus, eikenella corrodens, capnocytophaga sputigena and streptoco ... | 1997 | 9151641 |
| the role of heat shock protein, microbial and autoimmune agents in the aetiology of behçet's disease. | investigation of the aetiology of behçet's disease (bd) has focused predominantly on herpes simplex virus immunopathology, autoimmunity to oral mucosa or cross-reactive microbial antigens, and streptococcal infection. these aetiological factors might have a common denominator in microbial heat shock protein (hsp) which shows significant homology with the human mitochondrial hsp. indeed, the uncommon serotypes of streptococcus sanguis found in bd cross-react with the 65 kd hsp which also shares a ... | 1997 | 9203024 |
| the effect of smear layer on microbial coronal leakage of gutta-percha root fillings. | the aim of this in vitro study was to determine the effect of removal of the smear layer on canal obturation as measured by penetration of bacteria from a coronal direction. one hundred and twenty extracted human teeth with straight, single root canals were decoronated. the canals were prepared using the modified double-flared technique with balanced force under copious irrigation. the apical matrix was prepared to size 40 and apical patency subsequently confirmed with a size 15 file. the teeth ... | 1996 | 9206440 |
| [a case of severe odontogenic infection with allergic skin reaction for beta-lactam antibiotics]. | a 54-aged woman consulted with right buccal phlegmon caused by lower apical periodontitis. the primary chemotherapy using flomoxef induced allergic skin eruption. a decision was made for using clindamycin, levofloxacin and fosfomycin as chemotherapeutic agents. cultured organisms from the abscess revealed the mixed infection with streptococcus sanguis, veillonella sp., prevotella loescheii, wolinella spp. on 12th hospital day, her serum crp turned to negative. this case carried numerous implicat ... | 1997 | 9209128 |
| the role of immunoglobulin g and fibrinogen in platelet aggregation by streptococcus sanguis. | previous work has shown that the type strain of streptococcus sanguis, nctc 7863, induces aggregation of normal platelets by a complement-dependent mechanism. we investigated the roles of igg and fibrinogen in the aggregation process. plasma depleted of igg by passage through protein a-sepharose failed to support platelet aggregation, as did plasma absorbed at 0 degrees c with whole bacteria. however, absorption of plasma with a non-aggregating strain of s. sanguis, sk96, did not remove aggregat ... | 1997 | 9217171 |
| effects of acidification on growth and glycolysis of streptococcus sanguis and streptococcus mutans. | after carbohydrate intake, ph in dental plaque decreases rapidly and reaches about 4 within a few minutes. the acidification not only promotes demineralization of tooth surface but can also cause damage to bacteria in dental plaque. we, therefore, investigated the effect of acidification on the dental plaque bacteria streptococcus sanguis and streptococcus mutans. at ph 4.0 and 4.2, both growth and glycolytic activities in these streptococci were repressed. prolonged acidification (for 60 min at ... | 1997 | 9227129 |
| inhibition of purified enolases from oral bacteria by fluoride. | enolase activity in strains of oral streptococci previously has been found to be inhibited by 50% (ki) by fluoride concentrations ranging from 50 to 300 microm or more in the presence of 0.5 to 1.0 mm inorganic phosphate ions. in this study, enolase was extracted and partly purified by a two-step process from five oral bacterial species and the effect of fluoride on the kinetics of enolase examined. the molecular weight of the putative enolase proteins was 46-48 kda. the vmax values ranged from ... | 1997 | 9227132 |
| interaction between oral alpha-streptococci and group a streptococci in patients with tonsillitis. | the incidence of oral alpha-streptococci with inhibitory activity against group a streptococci, as a defense mechanism against bacterial infection in the oral cavity, was investigated in 141 patients with streptococcal tonsillitis. the study population included both children (n = 79) and adults (n = 62). infection by group a streptococci appeared to be more common in children than in adults, as the detection rates of inhibitory alpha-streptococci in healthy children (29.7%), as well as pediatric ... | 1997 | 9228858 |
| [value of mri with injection of gadolinium in the diagnosis of mitral ring abscess. apropos of a case]. | transoesophageal echocardiography is the investigation of choice for the diagnosis of valvular vegetations in infectious endocarditis but the diagnosis of an associated ring abscess is sometimes difficult especially in patients with valvular prostheses or calcifications which are in important source of artefacts. the authors report a case of mitral annular abscess confirmed at surgery and comment on the diagnostic and prognostic value of cardiac mri. mri showed an abnormal cavity directly relate ... | 1997 | 9232079 |
| pathway for uptake and degradation of x-prolyl tripeptides in streptococcus mutans va-29r and streptococcus sanguis atcc 10556. | the growth of streptococcus mutans and streptococcus sanguis in the oral environment requires that these micro-organisms be able to degrade salivary proteins and to assimilate the resulting peptides as an amino nitrogen source. our research is aimed at the definition of the proteolytic enzyme systems in these oral streptococci which allow them to utilize such substrates. in the present work, the nature of the hydrolytic activity expressed by s. mutans va-29r and s. sanguis atcc 10556 against x-p ... | 1997 | 9240384 |
| cerebrospinal fluid broth culture isolates: their significance for antibiotic treatment. | enriched broth medium is routinely used as a supplement for agar plate culture of cerebrospinal fluid (csf). to assess the clinical utility of broth cultures, 151 consecutive csf bacterial and fungal isolates obtained from 91 patients were retrospectively reviewed for the effect of results on treatment. treatment decisions associated with individual csf specimens for which isolates were recovered from thioglycollate broth only were compared with the treatment decisions associated with csf specim ... | 1997 | 9260764 |
| [the effects of lipoteichoic acid and calcium on the adherence of oral streptococci in vitro]. | in this study, the effects of lipoteichoic acid (lta) and calcium on adherence of oral streptococci in vitro were investigated. the results revealed that the adherence of streptococcus sobrinus to a glass surface was increased by the addition of calcium, in the condition of the same calcium ion concentration, lta inhibited adherence, suggesting that lta and the bacterial cell surface lta competed for the same site of calcium ion, providing some evidence to support calcium-bridging theory. the re ... | 1996 | 9275604 |
| streptococcus sanguis expresses a 150-kilodalton two-domain adhesin: characterization of several independent adhesin epitopes. | streptococcus sanguis binds to saliva-coated hydroxylapatite (sha), an in vitro model of the enamel pellicle. to learn if more than one adhesin functions during adhesion, 12 reactive monoclonal antibodies (mabs) were isolated by screening against both adhesive and nonadhesive strains. two of these mabs, 1.1 and 1.2, inhibited adhesion in a dose-dependent fashion, although maximum inhibition with either was only 37%. when these two mabs plus a polyclonal antibody to p1-like adhesin were combined, ... | 1997 | 9284157 |
| thermophysiology of streptococcus mutans and related lactic-acid bacteria. | the temperature ranges for growth of streptococcus mutans gs-5 and s. sobrinus 6715 were found to be very narrow, from about 30 to 47 degrees c, with optimal growth around 37 degrees c. thus, the organisms showed little potential to grow in the environment outside of the animal host. in contrast wider ranges were found for enterococcus hirae, s. rattus and s. sanguis. detailed study of s. mutans gs-5 showed that energetic coupling, reflected in yields of biomass per mol of glucose utilized, were ... | 1997 | 9298187 |
| comparative in-vitro activities of quinupristin-dalfopristin against gram-positive bloodstream isolates. | the in-vitro activity of quinupristin-dalfopristin was compared with those of vancomycin, teicoplanin, erythromycin, clarithromycin, rifampicin, imipenem, meropenem, ciprofloxacin and sparfloxacin against 414 bloodstream isolates of gram-positive cocci. quinupristin-dalfopristin inhibited strains of streptococcus pyogenes and streptococcus agalactiae at 0.12 mg/l, methicillin- and/or erythromycin-resistant staphylococcus aureus and staphylococcus epidermidis at 0.5 mg/l, staphylococcus haemolyti ... | 1997 | 9301986 |
| streptococcus sanguis bacteremia associated with cecal carcinoma: case report and review of the literature. | 1997 | 9317082 | |
| selection system for the isolation of in vivo activated promoters from endocarditis-causing viridans streptococci. | 1997 | 9331764 | |
| competence-pheromone in streptococcus sanguis. identification of the competence gene comc and the competence pheromone. | 1997 | 9331824 | |
| adherence of oral streptococci to an immobilized antimicrobial agent. | an antimicrobial agent, 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride, was immobilized on silica. interaction between the material (termed) oais) and various oral bacterial species were then studied. seven species of streptococcus and two actinomyces were investigated for their ability to adhere to this biomaterial. cell-surface hydrophobicity and zeta-potential were examined as well. analysis of extracted hydrophobic proteins which adhered to oais revealed that the adherence of ... | 1997 | 9347116 |
| effect of radiotherapy on the levels of secretory immunoglobulin a against indigenous and virulent streptococci. | it is well known that the frequency of upper respiratory infection is clinically increased after radiotherapy of the head and neck region. this study found higher antibacterial secretory immunoglobulin a (s-iga) activity against three indigenous streptococci (streptococcus mitis, s. salivarius, and s. sanguis i) and s. pneumoniae in patients who had undergone radiation therapy of the head and neck region than in control subjects. this showed no relation to the extent of the radiation field. comp ... | 1997 | 9374163 |
| "checkerboard" versus culture: a comparison between two methods for identification of subgingival microbiota. | the present study compared the "checkerboard" dna-dna hybridization methodology with culture techniques for the analysis of the composition of the subgingival microbiota. 70 subjects, presenting with a variety of periodontal conditions, contributed with a total of 283 subgingival plaque samples analyzed with respect to the following species: porphyromonas gingivalis, prevotella intermedia/prevotella nigrescens, fusobacterium nucleatum, campylobacter rectus, eikenella corrodens, bacteroides forsy ... | 1997 | 9395099 |
| on the rôle of human salivary micelle-like globules in bacterial agglutination. | the hypothesis to be tested in this in vitro study was that the salivary micelle-like globules (smgs) have a rôle in the agglutination of some oral bacteria. an attempt to determine the mechanisms for the interactions involved was also carried out. 4 laboratory and 4 native streptococci strains were tested. human whole (hws) and parotid (hps) saliva was collected from 4 subjects, and smgs were isolated from both salivas, and agglutination was recorded in the various bacterial suspensions over ti ... | 1997 | 9395114 |
| comparison of the subgingival microbiota of periodontally healthy and diseased adults in northern cameroon. | our study is the 1st report on subgingival microbiota in adult cameronians. the aim was to investigate, using the checkerboard dna-dna hybridization technique, the prevalence of 18 oral species in subgingival plaque samples obtained from sex- and age-matched cameronian adults with and without periodontal destruction. we also compared cultivation and the affirm dp test with the checkerboard technique in their capability to detect some selected species among the 18. 21 adult periodontitis patients ... | 1997 | 9402505 |
| [aerobic and anaerobic bacterial flora in chronic sinusitis in adults]. | the aim of the study was to analyse microbiologically samples obtained from 30 patients aged from 21 to 73 years treated for chronic sinusitis. aerobic bacteria only were isolated in 16 patients (53%), and anaerobic organisms only in 5 patients (17%). mixed aerobic and anaerobic isolates were recovered from 9 patients (30%). the isolated aerobic bacteria were as follows: streptococci from the species streptococcus salivarius, streptococcus anginosus, streptococcus group c, streptococcus sanguis, ... | 1997 | 9411078 |
| in vitro and in vivo antibacterial activities of gv129606, a new broad-spectrum trinem. | gv129606 is a new parenteral trinem antibiotic belonging to the beta-lactam class. it combines broad-spectrum activity (against gram-negative and -positive bacteria, aerobes and anaerobes), with high potency and resistance to beta-lactamases. comparative in vitro and in vivo antibacterial activities were determined for gv129606 against more than 400 recent clinical isolates (aerobes, including beta-lactamase producers, and anaerobes), using representative antibacterial agents (meropenem, piperac ... | 1997 | 9420050 |
| effects of triclosan and triclosan monophosphate on maximum specific growth rates, biomass and hydrolytic enzyme production of streptococcus sanguis and capnocytophaga gingivalis in continuous culture. | dental plaque species, streptococcus sanguis and capnocytophaga gingivalis, were grown in continuous culture with progressively increasing concentrations of triclosan or its phosphorylated derivative, triclosan monophosphate (tmp). for both organisms, the maximum specific growth rates decreased with increasing concentrations of triclosan or tmp until complete inhibition of growth occurred, which for s. sanguis was at 20 mg/l and 50 mg/l, and for c. gingivalis was at 10 mg/l and 5 mg/l for triclo ... | 1997 | 9421313 |
| a comprehensive genetic study of streptococcal immunoglobulin a1 proteases: evidence for recombination within and between species. | an analysis of 13 immunoglobulin a1 (iga1) protease genes (iga) of strains of streptococcus pneumoniae, streptococcus oralis, streptococcus mitis, and streptococcus sanguis was carried out to obtain information on the structure, polymorphism, and phylogeny of this specific protease, which enables bacteria to evade functions of the predominant ig isotype on mucosal surfaces. the analysis included cloning and sequencing of iga genes from s. oralis and s. mitis biovar 1, sequencing of an additional ... | 1998 | 9423856 |
| colonic perforation by ventriculoperitoneal shunt tubing: a case of suspected silicone allergy. | a case of colonic perforation by a ventriculoperitoneal shunt is presented in a patient with several previous complications associated with shunt tubing. | 1998 | 9428890 |
| anaerobic killing of oral streptococci by reduced, transition metal cations. | reduced, transition metal cations commonly enhance oxidative damage to cells caused by hydroperoxides formed as a result of oxygen metabolism or added externally. as expected, the cations fe2+ and cu+ enhanced killing of streptococcus mutans gs-5 by hydroperoxides. however, unexpectedly, they also induced lethal damage under fully anaerobic conditions in a glove box with no exposure to o2 or hydroperoxides from initial treatment with the cations. sensitivities to anaerobic killing by fe2+ varied ... | 1998 | 9435058 |
| sinusitis of dental origin. | acute or chronic sinusitis may be odontogenic. bacteria involved in odontogenic sinusitis are specific organisms associated with the teeth (streptococcus sanguis, streptococcus salivarius, streptococcus mutans, anaerobic germs). they are often secondary to an intrasinus foreign body following periodontitis. the treatment is both naso-sinusal and dental. cysts of the maxilla can also invade the sinus. in particular, radiculo-dental cysts (periapical) must be surgically excised, in some cases asso ... | 1997 | 9444378 |
| role of monocytes and bacteria in staphylococcus epidermidis endocarditis. | the endocardial vegetation which is formed in the course of bacterial endocarditis (be) contains tissue factor (tf)-dependent procoagulant activity. earlier studies showed that monocytes are the main source of tf in the vegetations. the tf activity (tfa) of vegetations isolated from streptococcus sanguis-infected rabbits depended on the numbers of bacteria as well as monocytes in the vegetation. in this study, we investigated whether for staphylococcus epidermidis, a frequent pathogen in be, an ... | 1998 | 9453594 |
| antimicrobial susceptibility of viridans group streptococci. | a total of 68 viridans group streptococci, including 31 streptococcus sanguis, 12 s. mitis, 3 s. salivarius, and 8 s. milleri from blood, and an additional 14 s. milleri from abscesses and normally sterile sites, were tested against penicillin, amoxicillin, cefazolin, ceftriaxone, meropenem, clindamycin, quinupristin/dalfopristin, rifampin, levofloxacin, ofloxacin, vancomycin, and gentamicin with the microdilution method. the susceptibility rates for s. sanguis were: penicillin, 74%; amoxicillin ... | 1997 | 9458986 |
| the effect of fluoride on streptococcus sanguis 7863 iga1 protease production and activity. | fluoride was found to affect the production of the bacterial iga1 protease but to have no effect on iga1 protease activity. the concentrations of fluoride that do affect streptococcus sanguis growth and iga1 protease production are higher than those normally seen in vivo under normal circumstances. the concentrations of fluoride in dental plaque following use of a fluoride rinse or dentifrice would be sufficient to reduce strep. sanguis iga1 protease production. | 1997 | 9460535 |
| extracts of prevotella intermedia and actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro. | in order to study the effects of sonicated extracts from prevotella intermedia, actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and other oral-related bacteria, as well as escherichia coli on bone formation, clone mc3t3-e1 cells, which have retained osteoblastic activity, were cultured with various bacterial extracts. the addition of the sonicated extracts from prevotella intermedia and actinobacillus actinomycetemcomitans decreased the alkaline phosphatase activity in a dose-dep ... | 1997 | 9467351 |
| differential toxic effects of lactate and acetate on the metabolism of streptococcus mutans and streptococcus sanguis. | experiments were conducted with streptococcus mutans nctc 10449 and streptococcus sanguis atcc 10556 to determine whether the acid end-products, lactate and acetate, were involved in the regulation of cellular growth and metabolism. the growth rate and culture biomass of both organisms was inhibited by the addition of lactate and acetate at concentrations as high as 200 mm to the cultures, although the final ph values of the lactate and acetate cultures were similar. in addition, the metabolic c ... | 1996 | 9467375 |
| time-dependent interfacial tension of whole saliva and saliva-bacteria mixes. | using a tensiometer in accordance with the drop volume principle, the surface tension decrease with time was determined for whole and for 2%, 10%, and 50% aqueous solutions of saliva from one healthy donor. the reduction of surface tension with time was also measured for 10% and 20% saliva solutions with added samples of streptococcus salivarius krf2, s. sanguis krf3, and actinomyces naeslundii 2t-55. the results show that 1) there is a time dependence of the surface tension reduction of both wh ... | 1997 | 9477032 |
| [control of buccal peroxidases by a bacterial nadh-hypothiocyanite oxidoreductase]. | oral peroxidases (myeloperoxidase, sialoperoxidase) catalyze thiocyanate peroxidation into hypothiocyanite which is bacteriostatic or bactericidal against numerous bacterial species. nadh-hypothiocyanite-oxidoreductase is thought to protect bacteria which can express it; up to now, this enzyme activity was never purified. the present study analyzes, on one hand, the susceptibility of periodontal bacteria against hypothiocyanite and, on the other hand, proposes a purification design for the nadh- ... | 1996 | 9491629 |
| iga protease produced by streptococcus sanguis and antibody production against iga protease in patients with behçet's disease. | the production of iga protease in twelve strains of streptococcus sanguis isolated from patients with behcet's disease (bd) was examined. protease activity was detected in 10 out of 12 strains. the protease was purified from one representative strain, s. sanguis 113-20, by employing rotofor and deae-sephacel chromatography. the molecular mass of the purified protease was approximately 100 kda, and it cleaved the proline-threonine site of the iga. both igg and iga titers against the cells (113-20 ... | 1997 | 9492177 |
| microbial complexes in subgingival plaque. | it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ... | 1998 | 9495612 |
| streptococcus sanguis bacteremia and colorectal cancer. | streptococcal bacteremia is an uncommon presentation for colorectal malignancy, yet most physicians are aware of the association between streptococcus bovis infection and these malignancies. many are unaware, however, that other streptococcal species are associated with colon and rectal cancers. in this case report and brief review, we highlight this association and discuss a case of streptococcus sanguis bacteremia associated with an early invasive rectal cancer. | 1998 | 9496877 |
| the interaction of phospholipid liposomes with bacteria and their use in the delivery of bactericides. | liposomes have been prepared from dipalmitoylphosphatidylcholine (dppc) incorporating the cationic lipids stearylamine (sa), dimethyldioctadecylammonium bromide (ddab) and dimethylaminoethane carbamoyl cholesterol (dcchol) and the anionic lipids dipalmitoylphosphatidylglycerol (dppg) and phosphatidylinositol (pi). their adsorption to biofilms of skin-associated bacteria (staphylococcus epidermidis and proteus vulgaris) and oral bacteria (streptococcus mutans and sanguis) has been investigated as ... | 1997 | 9524311 |
| host-pathogen interactions in bacterial endocarditis: streptococcal virulence in the host. | to identify streptococcal genes that are expressed during experimental endocarditis, we developed a promoter-less dual reporter gene-fusion (amy, cat) plasmid, pak36. chromosomal dna from s. gordonii v288 was digested with sau3a1. the resulting fragments were ligated into pak36. following transformation into s. gordonii, the library of random gene fusion clones was inoculated into a rabbit to induce experimental endocarditis. chloramphenicol treatment effected positive selection. upon euthanizat ... | 1997 | 9524444 |
| assessing the relationship between dental disease and coronary heart disease in elderly u.s. veterans. | several recent studies have shown a link between dental disease and coronary heart disease. the authors studied 320 u.s. veterans in a convenience sample to assess the relationship between oral health and systemic diseases among older people. they present cross-sectional data confirming that a statistically significant association exists between a diagnosis of coronary heart disease and certain oral health parameters, such as the number of missing teeth, plaque benzoyl-dl-arginine-naphthylamide ... | 1998 | 9529805 |
| whole-cell protein electrophoretic analysis of viridans streptococci: evidence for heterogeneity among streptococcus mitis biovars. | one hundred reference strains representing all species belonging to the different phylogenetic lineages of the viridans streptococci were examined by means of one-dimensional whole-organism protein electrophoresis. for most of the species examined, multiple strains characterized by dna-dna hybridization were included and, wherever described, representatives of different biochemical variants were analysed. most species were clearly differentiated. the data support the viewpoint that members of th ... | 1998 | 9542082 |
| a microbiological study of pre- and postoperative apicoectomy sites. | there is little information on the microbiology of periapical lesions, and no data on the residual microbial flora in the periapex, if any, after apicoectomy procedures. hence, 64 patients treated by apicoectomy procedures were prospectively studied to assess the bacterial flora in the periapex and to evaluate the residual bacteria in postoperative apicoectomy sites. of the 64 lesions studied, 14 (22%) were sterile and 50 (78%) yielded bacteria preoperatively. bacteria could be recovered from 28 ... | 1997 | 9552822 |
| extraction, assay, and analysis of antimicrobials from plants with activity against dental pathogens (streptococcus sp.) | many dental and periodontal diseases are largely a question of bacterial etiology. dental caries develop due to an increase of strongly acidogenic and aciduric gram-positive bacteria while common forms of periodontal disease are linked to anaerobic gram-negative bacteria in subgingival plaque. many plants and plant-derived antimicrobial components are used in folklore therapeutics for the treatment of periodontal disorders and for the purposes of oral hygiene. some have been evaluated for possib ... | 1998 | 9553834 |
| coronal leakage of obturated root canals after long-term storage using a polymicrobial marker. | this in vitro study investigated the effect of long-term storage on the coronal leakage of a microbial marker on teeth root filled with lateral condensation of cold gutta-percha and one of two sealers. sixty single-rooted teeth were prepared chemomechanically to a size 40 master apical file. the teeth were divided into two groups of 20 teeth each and obturated with gutta-percha using either apexit or tubliseal ewt sealer. the teeth were stored for 6 months in artificial saliva and tested for lea ... | 1997 | 9587271 |
| mutacin production by streptococcus mutans may promote transmission of bacteria from mother to child. | the production of bacteriocin-like inhibitory substances, mutacins, by mutans streptococci varies among isolates. to find if the degree of mutacin activity of an isolate was related to its transmission between mother and her child, 19 mothers and their 18-month- to 3-year-old children were sampled for their oral mutans streptococci. in addition, the stability of mutacin activity was studied with isolates from the mothers and with isolates from five unrelated 5-year-old children in 5- to 7-year f ... | 1998 | 9596721 |
| development of a novel in vitro co-culture system for studying host response to native bacterial antigens. | we have developed a novel co-culture system in which murine splenocytes are cultured with live bacteria in the presence of a bacteriostatic antibiotic. superantigens, like staphylococcal enterotoxin b (seb) are important factors in bacterial pathogenicity. research has shown that superantigens affect numerous immune cell types, either directly or indirectly, yet their involvement in pathogenic mechanisms remains poorly defined. in these studies, we utilize the co-culture system to study how supe ... | 1998 | 9617839 |
| [mycotic aneurysm in endocarditis lenta as the etiology of intraparenchymatous cerebral hemorrhage]. | case report: a 49-year-old male was admitted for left-side headache and mild speech defect. clinical examination showed a pansystolic murmur of mitral regurgitation and mild wernicke aphasia. in laboratory studies esr and crp were increased. computed tomography of brain revealed a left temporoparietal hematoma. echocardiographic examination demonstrated vegetations and mitral valve perforation. in blood cultures grew alpha-streptococci. cerebral angiography was performed and a fusiform aneurysm ... | 1998 | 9630815 |
| cloning and sequencing of bes-1 gene encoding the immunogenic antigen of streptococcus sanguis kth-1 isolated from the patients with behçet's disease. | in order to analyze the immunopathologic mechanisms of behçet's disease, the gene (bes-1) encoding a streptococcal antigen correlated with the disease was cloned and sequenced, and protein produced by this clone was identified by western immunoblotting using serum antibody from the patient. cellular dna of streptococcus (s.) sanguis serotype kth-1 (uncommon serotype 1, strain 113-20) from the patient was extracted and digested with ecori. the digested fragments were cloned into the cloning vecto ... | 1998 | 9638874 |
| binaphthalenone glycosides from african chewing sticks, diospyros lycioides. | our laboratory has engaged in the exploration of active antimicrobial principles present in chewing sticks commonly used by the african and middle eastern countries as a mechanical oral hygiene aid in place of tooth brushing. during this investigation, a methanol extract from the twigs of diospyros lycioides, a namibia tooth cleaning stick, demonstrated antimicrobial activity against common oral pathogens including streptococcus mutans and porphyromonas gingivalis (mics 2.5 and 0.156 mg/ml). sub ... | 1998 | 9644074 |