Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| mycofabricated biosilver nanoparticles interrupt pseudomonas aeruginosa quorum sensing systems. | quorum sensing (qs) is a chemical communication process that pseudomonas aeruginosa uses to regulate virulence and biofilm formation. disabling of qs is an emerging approach for combating its pathogenicity. silver nanoparticles (agnps) have been widely applied as antimicrobial agents against human pathogenic bacteria and fungi, but not for the attenuation of bacterial qs. here we mycofabricated agnps (mfagnps) using metabolites of soil fungus rhizopus arrhizus brs-07 and tested their effect on q ... | 2015 | 26347993 |
| genome analysis of quorum sensing cedecea neteri ssmd04 leads to identification of its novel signaling synthase (cnei), cognate receptor (cner) and an orphan receptor. | cedecea neteri is a very rare human pathogen. we have isolated a strain of c. neteri ssmd04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. using the biosensor chromobacterium violaceum cv026, we have demonstrated the presence of short chain n-acyl-homoserine lactone (ahl) type quorum sensing (qs) activity in c. neteri ssmd04. triple quadrupole lc/ms analysis revealed that c. neteri ssmd04 produced short chain n-butyryl-homoserine lactone (c4-hsl). with the a ... | 2015 | 26355540 |
| structural and functional studies of the pseudomonas aeruginosa minor pilin, pile. | many bacterial pathogens, including pseudomonas aeruginosa, use type iva pili (t4ap) for attachment and twitching motility. t4ap are composed primarily of major pilin subunits, which are repeatedly assembled and disassembled to mediate function. a group of pilin-like proteins, the minor pilins fimu and pilvwxe, prime pilus assembly and are incorporated into the pilus. we showed previously that minor pilin pile depends on the putative priming subcomplex pilvwx and the non-pilin protein pily1 for ... | 2015 | 26359492 |
| genome-wide rna sequencing analysis of quorum sensing-controlled regulons in the plant-associated burkholderia glumae pg1 strain. | burkholderia glumae pg1 is a soil-associated motile plant-pathogenic bacterium possessing a cell density-dependent regulation system called quorum sensing (qs). its genome contains three genes, here designated bgai1 to bgai3, encoding distinct autoinducer-1 (ai-1) synthases, which are capable of synthesizing qs signaling molecules. here, we report on the construction of b. glumae pg1 δbgai1, δbgai2, and δbgai3 mutants, their phenotypic characterization, and genome-wide transcriptome analysis usi ... | 2015 | 26362987 |
| regulation of gaca in pseudomonas chlororaphis strains shows a niche specificity. | the gacs/gaca two-component system plays a central role in the regulation of a broad range of biological functions in many bacteria. in the biocontrol organism pseudomonas chlororaphis, the gac system has been shown to positively control quorum sensing, biofilm formation, and phenazine production, but has an overall negative impact on motility. these studies have been performed with strains originated from the rhizosphere predominantly. to investigate the level of conservation between the gaca r ... | 2015 | 26379125 |
| interference of quorum sensing by delftia sp. vm4 depends on the activity of a novel n-acylhomoserine lactone-acylase. | turf soil bacterial isolate delftia sp. vm4 can degrade exogenous n-acyl homoserine lactone (ahl), hence it effectively attenuates the virulence of bacterial soft rot pathogen pectobacterium carotovorum subsp. carotovorum strain br1 (pcc br1) as a consequence of quorum sensing inhibition. | 2015 | 26384328 |
| urinary tract infection caused by chromobacterium violaceum. | chromobacterium violaceum, a proteobacterium, is a facultative anaerobe, which is generally present as the normal flora of water and soil in tropical and subtropical regions. the infection due to chromobacterium violaceum is rare but mostly fatal. it is responsible for causing fatal cases of septicemia, visceral abscesses, skin and soft tissue infections, meningitis, diarrhea, and rarely urinary tract infection. the bacteria has high propensity to spread causing sepsis. delayed proper treatment ... | 2015 | 26392784 |
| antibacterial and anti-quorum sensing molecular composition derived from quercus cortex (oak bark) extract. | quercus cortex (oak bark) has been used in european folk medicine since medieval times for treatment of diarrhea, stomatitis, pharyngitis and skin inflammations. its antimicrobial activity is a well-known therapeutic property of oak bark, and its novel anti-quorum sensing (qs) ability has also been described recently. in this study, we examined the bioactive compounds of quercus cortex extract and compared their direct antibacterial and regulatory anti-qs effects against chromobacterium violaceu ... | 2015 | 26393551 |
| hydnophytum formicarum jack ethanol extract modulates quorum sensing-controlled pathogenicity in pseudomonas aeruginosa. | the discovery of new mechanism to control microbial pathogenicity by quorum sensing modulation has generated the search for quorum sensing inhibitor from natural resources. the objective of this research was to evaluate the ability of hydnophytum formicarum jack (rubiaceae) ethanol extract to antagonize cell-to cell communication. pulverized h. formicarum tuber was macerated in ethyl alcohol 96% and evaporated to yield ethanol extract. a dillution technique using luria-bertani (lb) medium was us ... | 2015 | 26408889 |
| bioleaching of gold, copper and nickel from waste cellular phone pcbs and computer goldfinger motherboards by two aspergillus nigerstrains. | in an effort to develop alternate techniques to recover metals from waste electrical and electronic equipment (weee), this research evaluated the bioleaching efficiency of gold (au), copper (cu) and nickel (ni) by two strains of aspergillus niger in the presence of gold-plated finger integrated circuits found in computer motherboards (gficms) and cellular phone printed circuit boards (pcbs). these three metals were analyzed for their commercial value and their diverse applications in the industr ... | 2015 | 26413051 |
| bacterial danger sensing. | here we propose that bacteria detect and respond to threats posed by other bacteria via an innate immune-like process that we term danger sensing. we find support for this contention by reexamining existing literature from the perspective that intermicrobial antagonism, not opportunistic pathogenesis, is the major evolutionary force shaping the defensive behaviors of most bacteria. we conclude that many bacteria possess danger sensing pathways composed of a danger signal receptor and correspondi ... | 2015 | 26434507 |
| the quorum-sensing inhibiting effects of stilbenoids and their potential structure-activity relationship. | stilbenoids, known an important phytoalexins in plants, were renowned for their beneficial effects on cardiovascular, neurological and hepatic systems. in the present study, quorum sensing inhibition activity of ten stilbenoids were tested using chromobacterium violaceum cv026 as the bio-indicator strain and the structure-activity relationship was also investigated. among them, resveratrol (1), piceatannol (2) and oxyresveratrol (3) showed potential anti-qs activities. at the sub-mic concentrati ... | 2015 | 26453007 |
| overview of immunodeficiency disorders. | the spectrum of primary immunodeficiency disorders (pids) is expanding. it includes typical disorders that primarily present with defective immunity as well as disorders that predominantly involve other systems and show few features of impaired immunity. the rapidly growing list of new immunodeficiency disorders and treatment modalities makes it imperative for providers to stay abreast of the latest and best management strategies. this article presents a brief overview of recent clinical advance ... | 2015 | 26454309 |
| edwardsiella tarda esce (orf13 protein) is a type iii secretion system-secreted protein that is required for the injection of effectors, secretion of translocators, and pathogenesis in fish. | the type iii secretion system (t3ss) of edwardsiella tarda is crucial for its intracellular survival and pathogenesis in fish. the orf13 gene (esce) of e. tarda is located 84 nucleotides (nt) upstream of esrc in the t3ss gene cluster. we found that esce is secreted and translocated in a t3ss-dependent manner and that amino acids 2 to 15 in the n terminus were required for a completely functional t3ss in e. tarda. deletion of esce abolished the secretion of t3ss translocators, as well as the secr ... | 2015 | 26459509 |
| edwardsiella tarda esce (orf13 protein) is a type iii secretion system-secreted protein that is required for the injection of effectors, secretion of translocators, and pathogenesis in fish. | the type iii secretion system (t3ss) of edwardsiella tarda is crucial for its intracellular survival and pathogenesis in fish. the orf13 gene (esce) of e. tarda is located 84 nucleotides (nt) upstream of esrc in the t3ss gene cluster. we found that esce is secreted and translocated in a t3ss-dependent manner and that amino acids 2 to 15 in the n terminus were required for a completely functional t3ss in e. tarda. deletion of esce abolished the secretion of t3ss translocators, as well as the secr ... | 2015 | 26459509 |
| potent and selective modulation of the rhlr quorum sensing receptor by using non-native ligands: an emerging target for virulence control in pseudomonas aeruginosa. | pseudomonas aeruginosa uses n-acylated l-homoserine lactone signals and a triumvirate of luxr-type receptor proteins--lasr, rhlr, and qscr--for quorum sensing (qs). each of these receptors can contribute to qs activation or repression and, thereby, the control of myriad virulence phenotypes in this pathogen. lasr has traditionally been considered to be at the top of the qs receptor hierarchy in p. aeruginosa; however, recent reports suggest that rhlr plays a more prominent role in infection than ... | 2015 | 26460240 |
| violacein, an indole-derived purple-colored natural pigment produced by janthinobacterium lividum, inhibits the growth of head and neck carcinoma cell lines both in vitro and in vivo. | violacein (vio; 3-[1,2-dihydro-5-(5-hydroxy-1h-indol-3-yl)-2-oxo-3h-pyrrol-3-ylidene]-1,3-dihydro-2h-indol-2-one), an indole-derived purple-colored pigment, produced by a limited number of gram-negative bacteria species, including chromobacterium violaceum and janthinobacterium lividum, has been demonstrated to have anti-cancer activity, as it interferes with survival transduction signaling pathways in different cancer models. head and neck carcinoma (hnc) represents the sixth most common and on ... | 2016 | 26462840 |
| the effects of allium sativum extracts on biofilm formation and activities of six pathogenic bacteria. | garlic is considered a rich source of many compounds, which shows antimicrobial effects. the ability of microorganisms to adhere to both biotic and abiotic surfaces and to form biofilm is responsible for a number of diseases of chronic nature, demonstrating extremely high resistance to antibiotics. bacterial biofilms are complex communities of sessile microorganisms, embedded in an extracellular matrix and irreversibly attached to various surfaces. | 2015 | 26464762 |
| discovery of a new polyhydroxyalkanoate synthase from limestone soil through metagenomic approach. | pha synthase (phac) is the key enzyme in the production of biodegradable plastics known as polyhydroxyalkanoate (pha). nevertheless, most of these enzymes are isolated from cultivable bacteria using traditional isolation method. most of the microorganisms found in nature could not be successfully cultivated due to the lack of knowledge on their growth conditions. in this study, a culture-independent approach was applied. the presence of phac genes in limestone soil was screened using primers tar ... | 2016 | 26467694 |
| exposure to sub-inhibitory concentrations of cefotaxime enhances the systemic colonization of salmonella typhimurium in balb/c mice. | it has been proposed that sub-inhibitory concentrations of antibiotics play a role in virulence modulation. in this study, we evaluated the ability of salmonella enterica serovar typhimurium (hereafter s. typhimurium) to colonize systemically balb/c mice after exposure to a sub-inhibitory concentration of cefotaxime (ctx). in vivo competition assays showed a fivefold increase in systemic colonization of ctx-exposed bacteria when compared to untreated bacteria. to identify the molecular mechanism ... | 2015 | 26468132 |
| romidepsin targets multiple survival signaling pathways in malignant t cells. | romidepsin is a cyclic molecule that inhibits histone deacetylases. it is food and drug administration-approved for treatment of cutaneous and peripheral t-cell lymphoma, but its precise mechanism of action against malignant t cells is unknown. to better understand the biological effects of romidepsin in these cells, we exposed peer and supt1 t-cell lines, and a primary sample from t-cell lymphoma patient (patient j) to romidepsin. we then examined the consequences in some key oncogenic signalin ... | 2015 | 26473529 |
| chromobacterium violaceum sepsis: rethinking conventional therapy to improve outcome. | chromobacterium violaceum (c. violaceum) is a facultative anaerobic gram-negative bacterium found in soil and water, especially in tropical and subtropical areas. although infection in humans is rare, it is associated with significant morbidity. the bacterium is known for its resistance to multiple antimicrobials, and the possibility of relapse and reinfection. presence of bacteremia, disseminated infection, and ineffective antimicrobial agents are predictors of mortality. | 2015 | 26477750 |
| bacterial quorum sensing inhibition activity of the traditional chinese herbs, ficus carica l. and perilla frutescens. | quorum sensing (qs), as the basis of bacterial cell-to-cell communication, is a promising approach to reduce the incidence of multidrug resistance. the objective of this study was to search for novel quorum sensing inhibitors from plants and control detrimental infections. | 2014 | 26496663 |
| influence of salmonella enterica serovar typhimurium ssrb on colonization of eastern oysters (crassostrea virginica) as revealed by a promoter probe screen. | although salmonella has been isolated from 7.4 to 8.6% of domestic raw oysters, representing a significant risk for food-borne illness, little is known about the factors that influence their initial colonization by salmonella. this study tested the hypothesis that specific regulatory changes enable a portion of the invading salmonella population to colonize oysters. an in vivo promoter probe library screen identified 19 unique regions as regulated during colonization. the mutants in the nearest ... | 2015 | 26497459 |
| influence of salmonella enterica serovar typhimurium ssrb on colonization of eastern oysters (crassostrea virginica) as revealed by a promoter probe screen. | although salmonella has been isolated from 7.4 to 8.6% of domestic raw oysters, representing a significant risk for food-borne illness, little is known about the factors that influence their initial colonization by salmonella. this study tested the hypothesis that specific regulatory changes enable a portion of the invading salmonella population to colonize oysters. an in vivo promoter probe library screen identified 19 unique regions as regulated during colonization. the mutants in the nearest ... | 2015 | 26497459 |
| asymptomatic bacteriuria caused by chromobacterium violaceum in an immunocompetent adult. | because of increasing antimicrobial resistance, the treatment of the asymptomatic bacteriuria is not considered except in specific circumstances like during pregnancy or before invasive urologic procedures. we are reporting a first case of asymptomatic bacteriuria caused by chromobacterium violaceum in a 16-year-old male. with the reporting of the c. violaceum which is notorious for its high propensity for hematogenous dissemination causing fatal sepsis (with reported mortality rate up to 65-80% ... | 2015 | 26504464 |
| directed evolution and resolution mechanism of 1, 3-propanediol oxidoreductase from klebsiella pneumoniae toward higher activity by error-prone pcr and bioinformatics. | 1, 3-propanediol oxidoreductase (pdor) is a key enzyme in glycerol bioconversion to 1,3-propanediol (1, 3-pd) which is a valuable chemical and one of the six new petrochemical products. we used error-prone pcr and activity screening to identify mutants of klebsiella pneumoniae (k. pneumoniae) pdor with improved activity. the activity of one of the identified mutants, pdor'-24, which includes a single mutation, a199s, was 48 u/mg, 4.9 times that of the wild-type enzyme. molecular docking was perf ... | 2015 | 26528716 |
| tea polyphenols as an antivirulence compound disrupt quorum-sensing regulated pathogenicity of pseudomonas aeruginosa. | green tea, a water extract of non-fermented leaves of camellia sinensis l., is one of the nonalcoholic beverages in china. it is becoming increasingly popular worldwide, because of its refreshing, mild stimulant and medicinal properties. here we examined the quorum sensing inhibitory potentials of tea polyphenols (tp) as antivirulence compounds both in vitro and in vivo. biosensor assay data suggested minimum inhibitory concentrations (mics) of tp against selected pathogens were 6.25 ~ 12.5 mg/m ... | 2015 | 26548447 |
| 2-furaldehyde diethyl acetal from tender coconut water (cocos nucifera) attenuates biofilm formation and quorum sensing-mediated virulence of chromobacterium violaceum and pseudomonas aeruginosa. | the aim of this study was to evaluate the anti-biofilm and quorum sensing inhibitory (qsi) potential of tender coconut water (tcw) against chromobacterium violaceum and pseudomonas aeruginosa. tcw significantly inhibited the qs regulated violacein, virulence factors and biofilm production without affecting their growth. qrt-pcr analysis revealed the down-regulation of autoinducer synthase, transcriptional regulator and virulence genes. mass-spectrometric analysis of a petroleum ether extract of ... | 2015 | 26571230 |
| inflammasomes coordinate pyroptosis and natural killer cell cytotoxicity to clear infection by a ubiquitous environmental bacterium. | defective neutrophils in patients with chronic granulomatous disease (cgd) cause susceptibility to extracellular and intracellular infections. microbes must first be ejected from intracellular niches to expose them to neutrophil attack, so we hypothesized that inflammasomes detect certain cgd pathogens upstream of neutrophil killing. here, we identified one such ubiquitous environmental bacterium, chromobacterium violaceum, whose extreme virulence was fully counteracted by the nlrc4 inflammasome ... | 2015 | 26572063 |
| hif-1α pathway: role, regulation and intervention for cancer therapy. | hypoxia-inducible factor-1 (hif-1) has been recognized as an important cancer drug target. many recent studies have provided convincing evidences of strong correlation between elevated levels of hif-1 and tumor metastasis, angiogenesis, poor patient prognosis as well as tumor resistance therapy. it was found that hypoxia (low o2 levels) is a common character in many types of solid tumors. as an adaptive response to hypoxic stress, hypoxic tumor cells activate several survival pathways to carry o ... | 2015 | 26579469 |
| comparative metagenomics reveals impact of contaminants on groundwater microbiomes. | to understand patterns of geochemical cycling in pristine versus contaminated groundwater ecosystems, pristine shallow groundwater (fw301) and contaminated groundwater (fw106) samples from the oak ridge integrated field research center (or-ifrc) were sequenced and compared to each other to determine phylogenetic and metabolic difference between the communities. proteobacteria (e.g., burkholderia, pseudomonas) are the most abundant lineages in the pristine community, though a significant proporti ... | 2015 | 26583008 |
| amination of ω-functionalized aliphatic primary alcohols by a biocatalytic oxidation-transamination cascade. | amination of non-activated aliphatic fatty alcohols to the corresponding primary amines was achieved through a five-enzyme cascade reaction by coupling a long-chain alcohol oxidase from aspergillus fumigatus (lcao_af) with a ω-transaminase from chromobacterium violaceum (ω-ta_cv). the alcohol was oxidized at the expense of molecular oxygen to yield the corresponding aldehyde, which was subsequently aminated by the plp-dependent ω-ta to yield the final primary amine product. the overall cascade w ... | 2015 | 26583050 |
| tannin-rich fraction from pomegranate rind inhibits quorum sensing in chromobacterium violaceum and biofilm formation in escherichia coli. | pomegranate rind has been found to inhibit numerous pathogens, mostly attributed to its tannin fraction. the present study was conducted to investigate the quorum sensing (qs) inhibition effect of tannin-rich fraction from pomegranate rind (tfpr) by using an indicator strain chromobacterium violaceum. meanwhile, its effect on biofilm formation and motility of escherichia coli was evaluated. it was shown that tfpr inhibited qs-regulated violacein pigment production. biofilm formation and motility ... | 2016 | 26594817 |
| [the effect of introduction of the heterologous gene encoding the n-acyl-homoserine lactonase (aiia) on the properties of burkholderia cenocepacia 370]. | to study the role of quorum sensing (qs) regulation in the control of the cellular processes of burkholderia cenocepacia 370, plasmid pme6863 was transferred into its cells. the plasmid contains a heterologous gene encoding for aiia n-acyl-homoserine lactonase, which degrades the signaling molecules of the qs system of n-acyl-homoserine lactones (ahl). an absence or reduction of ahl in the culture was revealed with the biosensors chromobacterium violaceum cv026 and agrobacterium tumifaciens nt1/ ... | 2015 | 26601485 |
| a rare case of ecthyma gangrenosum caused by proteus vulgaris and candida albicans in a patient with castleman disease. | 2015 | 26618137 | |
| detection of quorum sensing molecules and biofilm formation in ralstonia solanacearum. | many bacteria use small diffusible signaling molecules to communicate each other termed as quorum sensing (qs). most gram-negative bacteria use acyl homoserine lactone (ahl) as qs signal molecules. using these signaling molecules, bacteria are able to express specific genes in response to population density. this work aimed to detect the production of qs signal molecules and biofilm formation in ralstonia solanacearum isolated from various diseased tomato plants with symptoms of bacterial wilt. ... | 2016 | 26620535 |
| non-pathogenic microflora of a spring water with regenerative properties. | the comano spring water (comano, italy) has been demonstrated to improve skin regeneration, not only by increasing keratinocyte proliferation and migration, but also by modulating the regenerated collagen and elastic fibers in the dermis. however, such biological properties may not be entirely explained by its mineral composition only. as the non-pathogenic bacterial populations have demonstrated an active role in different biological processes, the potential presence of non-pathogenic bacterial ... | 2015 | 26623012 |
| proteomic analysis of chromobacterium violaceum and its adaptability to stress. | chromobacterium violaceum (c. violaceum) occurs abundantly in a variety of ecosystems, including ecosystems that place the bacterium under stress. this study assessed the adaptability of c. violaceum by submitting it to nutritional and ph stresses and then analyzing protein expression using bi-dimensional electrophoresis (2-de) and maldi mass spectrometry. | 2015 | 26627076 |
| engineered production of tryprostatins in e. coli through reconstitution of a partial ftm biosynthetic gene cluster from aspergillus sp. | tryprostatin a and b are indole alkaloid-based fungal products that inhibit mammalian cell cycle at the g2/m phase. they are biosynthetic intermediates of fumitremorgins produced by a complex pathway involving a nonribosomal peptide synthetase (ftma), a prenyltransferase (ftmb), a cytochrome p450 hydroxylase (ftmc), an o-methyltransferase (ftmd), and several additional enzymes. a partial fumitremorgin biosynthetic gene cluster (ftmabcd) from aspergillus sp. was reconstituted in escherichia coli ... | 2014 | 26640821 |
| engineered production of tryprostatins in e. coli through reconstitution of a partial ftm biosynthetic gene cluster from aspergillus sp. | tryprostatin a and b are indole alkaloid-based fungal products that inhibit mammalian cell cycle at the g2/m phase. they are biosynthetic intermediates of fumitremorgins produced by a complex pathway involving a nonribosomal peptide synthetase (ftma), a prenyltransferase (ftmb), a cytochrome p450 hydroxylase (ftmc), an o-methyltransferase (ftmd), and several additional enzymes. a partial fumitremorgin biosynthetic gene cluster (ftmabcd) from aspergillus sp. was reconstituted in escherichia coli ... | 2014 | 26640821 |
| maniwamycins: new quorum-sensing inhibitors against chromobacterium violaceum cv026 were isolated from streptomyces sp. toho-m025. | quorum sensing is an important microbial signaling system that controls the expression of many virulence genes. maniwamycins c-f, new compounds and quorum-sensing inhibitors, were isolated from the culture broth of streptomyces sp. toho-m025 using a silica gel column and preparative hplc. the structures of maniwamycins were elucidated by spectroscopic analyses, including nmr. the compounds each have an azoxy moiety. all maniwamycins inhibited violacein synthesis, which is controlled by quorum se ... | 2016 | 26648117 |
| targeting quorum sensing by designing azoline derivatives to inhibit the n-hexanoyl homoserine lactone-receptor cvir: synthesis as well as biological and theoretical evaluations. | to counteract bacterial resistance, we investigated the interruption of quorum sensing mediated by non-classical bioisosteres of the n-hexanoyl homoserine lactone with an azoline core. for this purpose, a set of selected 2-substituted azolines was synthesized, establishing the basis for a new protocol to synthesize 2-amino imidazolines. the synthesized compounds were evaluated as inhibitors of violacein production in chromobacterium violaceum. theoretical studies on bioisostere-protein interacti ... | 2015 | 26654469 |
| smi2-mediated dimerization of indolylbutenones and synthesis of the myxobacterial natural product indiacen b. | the synthesis and reactivity of indole derivatives substituted in the benzene section was studied. starting materials 4- and 6-iodoindole were conveniently prepared via the batcho-leimgruber route and purified by sublimation. novel vicinally indolyl-substituted cyclopentanols with unexpected cis-configuration were formed by smi2-mediated reductive dimerization of a 4-(indol-6-yl)butenone, obtained by heck reaction. the two indolyl units appear to chelate sm(ii)/(iii) leading to a gauche-type arr ... | 2015 | 26664588 |
| potential of plant essential oils and their components in animal agriculture - in vitro studies on antibacterial mode of action. | the antimicrobial activity of essential oils and their components has been recognized for several years. essential oils are produced as secondary metabolites by many plants and can be distilled from all different portions of plants. the recent emergence of bacteria resistant to multiple antibiotics has spurred research into the use of essential oils as alternatives. recent research has demonstrated that many of these essential oils have beneficial effects for livestock, including reduction of fo ... | 2015 | 26664964 |
| distribution in different organisms of amino acid oxidases with fad or a quinone as cofactor and their role as antimicrobial proteins in marine bacteria. | amino acid oxidases (aaos) catalyze the oxidative deamination of amino acids releasing ammonium and hydrogen peroxide. several kinds of these enzymes have been reported. depending on the amino acid isomer used as a substrate, it is possible to differentiate between l-amino acid oxidases and d-amino acid oxidases. both use fad as cofactor and oxidize the amino acid in the alpha position releasing the corresponding keto acid. recently, a novel class of aaos has been described that does not contain ... | 2015 | 26694422 |
| characterization of n-acylhomoserine lactones produced by bacteria isolated from industrial cooling water systems. | the cooling water systems are used to remove heat generated in the various industries. biofouling of the cooling water systems causes blocking of condenser pipes and the heat exchanger tubes. in many gram-negative bacteria, n-acylhomoserine lactone (ahl) are used as quorum-sensing signal molecule and associated with biofilm formation. to investigate the relationship between quorum sensing and biofouling in the cooling water system, we isolated a total of 192 bacterial strains from the five cooli ... | 2016 | 26729121 |
| characterization of n-acylhomoserine lactones produced by bacteria isolated from industrial cooling water systems. | the cooling water systems are used to remove heat generated in the various industries. biofouling of the cooling water systems causes blocking of condenser pipes and the heat exchanger tubes. in many gram-negative bacteria, n-acylhomoserine lactone (ahl) are used as quorum-sensing signal molecule and associated with biofilm formation. to investigate the relationship between quorum sensing and biofouling in the cooling water system, we isolated a total of 192 bacterial strains from the five cooli ... | 2016 | 26729121 |
| a plasmid-transposon hybrid mutagenesis system effective in a broad range of enterobacteria. | random transposon mutagenesis is a powerful technique used to generate libraries of genetic insertions in many different bacterial strains. here we develop a system facilitating random transposon mutagenesis in a range of different gram-negative bacterial strains, including pectobacterium atrosepticum, citrobacter rodentium, serratia sp. atcc39006, serratia plymuthica, dickeya dadantii, and many more. transposon mutagenesis was optimized in each of these strains and three studies are presented t ... | 2015 | 26733980 |
| bioactivities by a crude extract from the greenlandic pseudomonas sp. in5 involves the nonribosomal peptides, nunamycin and nunapeptin. | background. bioactive microbial metabolites provide a successful source of novel compounds with pharmaceutical potentials. the bacterium pseudomonas sp. in5 is a biocontrol strain isolated from a plant disease suppressive soil in greenland, which produces two antimicrobial nonribosomal peptides (nrps), nunapeptin and nunamycin. methods. in this study, we used in vitro antimicrobial and anticancer bioassays to evaluate the potential bioactivities of both a crude extract derived from pseudomonas s ... | 2015 | 26734508 |
| the mysterious orphans of mycoplasmataceae. | the length of a protein sequence is largely determined by its function. in certain species, it may be also affected by additional factors, such as growth temperature or acidity. in 2002, it was shown that in the bacterium escherichia coli and in the archaeon archaeoglobus fulgidus, protein sequences with no homologs were, on average, shorter than those with homologs (bmc evol biol 2:20, 2002). it is now generally accepted that in bacterial and archaeal genomes the distributions of protein length ... | 2016 | 26747447 |
| chromobacterium violaceum isolated from a wound sepsis: a case study from nepal. | chromobacterium violaceum is a facultative anaerobic, gram-negative rod, prevalent in tropical and subtropical regions. it enters through the skin injury and is capable of causing severe systemic infections leading to septic shock and multiorgan failure. it has been reported by few authors across the world but this is probably the first case of chromobacterium violaceum isolated from wound sepsis from nepal. in this study, a pus sample from the infection of a prick injury in the left middle fing ... | 2015 | 26788383 |
| performances and reliability of bruker microflex lt and vitek ms maldi-tof mass spectrometry systems for the identification of clinical microorganisms. | in clinical microbiology laboratories, routine microbial identification is mostly performed using culture based methodologies requiring 24 to 72 hours from culturing to identification. matrix assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) technology has been established as a cost effective, reliable, and faster alternative identification platform. in this study, we evaluated the reliability of the two available maldi-tof ms systems for their routine clinical ... | 2015 | 26793718 |
| chromobacterium violaceum adaptation to low-phosphate conditions. | chromobacterium violaceum is a free-living bacterium that inhabits low-nutrient environments such as the amazon basin. bacteria respond to phosphate (pi) shortage by expressing a range of genes involved in pi uptake and assimilation, known as the pho regulon. several pho regulon genes have been annotated in the genome of c. violaceum. here we show that c. violaceum is extremely well adapted to low-pi conditions. remarkably, this bacterium is able to grow in media containing only traces of pi. th ... | 2016 | 26793969 |
| reassessing the evolutionary importance of inflammasomes. | inflammasomes monitor the cytosol for microbial contamination or perturbation and, thus, are predicted to provide potent defense against infection. however, the compendium of data from murine infection models suggests that inflammasomes merely delay the course of disease, allowing the host time to mount an adaptive response. interpretations of such results are confounded by inflammasome-evasion strategies of vertebrate-adapted pathogens. conversely, environmental opportunistic pathogens have not ... | 2016 | 26802061 |
| potential use of histone deacetylase inhibitors in cancer therapy. | epigenetics is a branch of science that focuses on mechanisms related to control and modification of expression of genetic material without any changes to its sequences. such mechanisms include post-translational modifications of histones. it is widely known that carcinogenesis is related to hypoacetylation of genes that influence apoptosis, the cell cycle, cell signaling, the immunologic response, angiogenesis and occurrence of metastasis. currently conducted research focuses on several strateg ... | 2015 | 26843838 |
| dissolved organic carbon from the upper rio negro protects zebrafish (danio rerio) against ionoregulatory disturbances caused by low ph exposure. | the so-called "blackwaters" of the amazonian rio negro are rich in highly coloured dissolved organic carbon (doc), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. however these blackwaters support 8% of the world's ichthyofauna. we tested the hypothesis that native doc provides protection against ionoregulatory dysfunction in this extreme environment. docs were isolated by reverse-osmosis from two rio negro sites. physico-chemical characteriza ... | 2016 | 26853589 |
| quorum sensing activity of citrobacter amalonaticus l8a, a bacterium isolated from dental plaque. | cell-cell communication is also known as quorum sensing (qs) that happens in the bacterial cells with the aim to regulate their genes expression in response to increased cell density. in this study, a bacterium (l8a) isolated from dental plaque biofilm was identified as citrobacter amalonaticus by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry (ms). its n-acylhomoserine-lactone (ahl) production was screened by using two types of ahl biosensors namely chr ... | 2016 | 26860259 |
| rosa damascena as holy ancient herb with novel applications. | rosa damascena as an ornamental plant is commonly known as "gole mohammadi" in iran. iranian people have been called this plant, the flower of prophet "mohammad". r. damascena is traditionally used for treatment of abdominal and chest pains, strengthening the heart, menstrual bleeding, digestive problems and constipation. this paper reviews the ethnopharmacology, phytochemistry and pharmaceutical investigations on r. damascena. all relevant databases and local books on ethnopharmacology of r. da ... | 2015 | 26870673 |
| rosa damascena as holy ancient herb with novel applications. | rosa damascena as an ornamental plant is commonly known as "gole mohammadi" in iran. iranian people have been called this plant, the flower of prophet "mohammad". r. damascena is traditionally used for treatment of abdominal and chest pains, strengthening the heart, menstrual bleeding, digestive problems and constipation. this paper reviews the ethnopharmacology, phytochemistry and pharmaceutical investigations on r. damascena. all relevant databases and local books on ethnopharmacology of r. da ... | 2015 | 26870673 |
| kinetic studies on cpha mutants reveal the role of the p158-p172 loop in activity versus carbapenems. | site-directed mutagenesis of cpha indicated that prolines in the p158-p172 loop are essential for the stability and the catalytic activity of subclass b2 metallo-β-lactamases against carbapenems. the sequential substitution of proline led to a decrease of the catalytic efficiency of the variant compared to the wild-type (wt) enzyme but also to a higher affinity for the binding of the second zinc ion. | 2016 | 26883708 |
| pathogens in ornamental waters: a pilot study. | in parks, ornamental waters of easy access and populated with animals are quite attractive to children and yet might hide threats to human health. the present work focuses on the microbiota of the ornamental waters of a lisboa park, characterized during 2015. the results show a dynamic microbiota integrating human pathogens such as klebsiella pneumoniae, aeromonas spp. and enterobacter spp., and also antibiotic resistant bacteria. k. pneumoniae and aeromonas spp. were present as planktonic and b ... | 2016 | 26891309 |
| the spectrum of chromobacterium violaceum infections from a single geographic location. | chromobacterium violaceum is a bacterium associated with soil and water exposure in tropical regions and causes rare and serious clinical infections that are often fatal. we reviewed the demographic and clinical details of 28 patients with c. violaceum detected over 15 years from 2000 to 2015, from the top end of the northern territory. of these patients, 18 had infections attributable toc. violaceum patients with infections were more commonly male (55.6%), and in the 16- to 60-year (61.1%) age ... | 2016 | 26903614 |
| bacterial-plant-interactions: approaches to unravel the biological function of bacterial volatiles in the rhizosphere. | rhizobacteria produce an enormous amount of volatile compounds, however, the function of these metabolites is scarcely understood. investigations evaluating influences on plants performed in various laboratories using individually developed experimental setups revealed different and often contradictory results, e.g., ranging from a significant plant growth promotion to a dramatic suppression of plant development. in addition to these discrepancies, these test systems neglected properties and com ... | 2016 | 26903987 |
| quorum sensing: an under-explored phenomenon in the phylum actinobacteria. | quorum sensing is known to play a major role in the regulation of secondary metabolite production, especially, antibiotics, and morphogenesis in the phylum actinobacteria. although it is one of the largest bacterial phylum, only 25 of the 342 genera have been reported to use quorum sensing. of these, only nine have accompanying experimental evidence; the rest are only known through bioinformatic analysis of gene/genome sequences. it is evident that this important communication mechanism is not e ... | 2016 | 26904007 |
| quorum sensing inhibitory potential and molecular docking studies of sesquiterpene lactones from vernonia blumeoides. | the increasing incidence of multidrug-resistant gram-negative bacterial pathogens has focused research on the suppression of bacterial virulence via quorum sensing inhibition strategies, rather than the conventional antimicrobial approach. the anti-virulence potential of eudesmanolide sesquiterpene lactones previously isolated from vernonia blumeoides was assessed by inhibition of quorum sensing and in silico molecular docking. inhibition of quorum sensing-controlled violacein production in chro ... | 2016 | 26920717 |
| detection of hydrogen cyanide from oral anaerobes by cavity ring down spectroscopy. | hydrogen cyanide (hcn) has been recognized as a potential biomarker for non-invasive diagnosis of pseudomonas aeruginosa infection in the lung. however, the oral cavity is a dominant production site for exhaled hcn and this contribution can mask the hcn generated in the lung. it is thus important to understand the sources of hcn production in the oral cavity. by screening of oral anaerobes for hcn production, we observed that the genus of porphyromonas, prevotella and fusobacterium generated low ... | 2016 | 26940198 |
| draft genome sequence of chromobacterium violaceum strain cv017. | we announce the draft genome sequence for chromobacterium violaceum strain cv017, used as a model and tool to understand acyl-homoserine lactone-dependent quorum sensing. the assembly consists of 4,774,638-bp contained in 211 scaffolds. | 2016 | 26941151 |
| genome sequence of enterobacter sp. st3, a quorum sensing bacterium associated with marine dinoflagellate. | phycosphere environment is a typical marine niche, harbor diverse populations of microorganisms, which are thought to play a critical role in algae host and influence mutualistic and competitive interactions. understanding quorum sensing-based acyl-homoserine lactone (ahl) language may shed light on the interaction between algal-associated microbial communities in the native environment. in this work, we isolated an epidermal bacterium (was tentatively named enterobacter sp. st3, and deposited i ... | 2016 | 26981407 |
| comparison of nested-multiplex, taqman & sybr green real-time pcr in diagnosis of amoebic liver abscess in a tertiary health care institute in india. | amoebiasis is a common parasitic infection caused by entamoeba histolytica and amoebic liver abscess (ala) is the most common extraintestinal manifestation of amoebiasis. the aim of this study was to standardise real-time pcr assays (taqman and sybr green) to detect e. histolytica from liver abscess pus and stool samples and compare its results with nested-multiplex pcr. | 2016 | 26997014 |
| antagonistic potential of pseudomonas graminis 49m against erwinia amylovora, the causal agent of fire blight. | in a previous study (mikiciński et al. in eur j plant pathol, doi: 10.1007/s10658-015-0837-y , 2015), we described the characterization of novel strain 49m of pseudomonas graminis, isolated from the phyllosphere of apple trees in poland showing a good protective activity against fire blight on different organs of host plants. we now report investigations to clarify the basis for this activity. strain 49m was found to produce siderophores on a medium containing complex cas-fe(3+) and hdtma, but w ... | 2016 | 27002332 |
| draft genome sequence of providencia sneebia strain st1, a quorum sensing bacterium associated with marine microalgae. | providencia sneebia strain st1 is a symbiotic bacterium (belonging to phylum gammaproteobacteria) with marine microalgae. this bacterium exhibits the ability to produce n-acyl homoserine lactone signal molecule. to date, no genome that originates from marine providencia spp. has been reported. in this study, we present the genome sequence of this strain. it has a genome size of 4.89 m, with 19 contigs and an average g+c of 51.97%. the function of 4,631 proteins was predicted, and 3,652 proteins ... | 2016 | 27026792 |
| tracking change: a look at the ecological footprint of antibiotics and antimicrobial resistance. | among the class of pollutants considered as 'emerging contaminants', antibiotic compounds including drugs used in medical therapy, biocides and disinfectants merit special consideration because their bioactivity in the environment is the result of their functional design. antibiotics can alter the structure and function of microbial communities in the receiving environment and facilitate the development and spread of resistance in critical species of bacteria including pathogens. methanogenesis, ... | 2013 | 27029298 |
| the search for violacein-producing microbes to combat batrachochytrium dendrobatidis: a collaborative research project between secondary school and college research students. | in this citizen science-aided, college laboratory-based microbiology research project, secondary school students collaborate with college research students on an investigation centered around bacterial species in the local watershed. this study specifically investigated the prevalence of violacein-producing bacterial isolates, as violacein has been demonstrated as a potential bioremediation treatment for outbreaks of the worldwide invasive chytrid, batrachochytrium dendrobatidis (bd). the impact ... | 2016 | 27047594 |
| secondary metabolites from the culture of the marine sponge-associated fungi talaromyces tratensis and sporidesmium circinophorum. | wortmin (1), meso-1,4-bis(4-methoxybenzyl)-2,3-butanediol (2), and a new isocoumarin derivative tratenopyrone (3) were isolated from the marine sponge-associated fungus talaromyces tratensis kufa 0091. a new diphenyl ether derivative, circinophoric acid (4), was isolated, together with the previously reported anthraquinones catenarin and physcion, the benzophenone monomethylsoluchrin, and β-ergosterol-5,8-endoperoxide, from the marine sponge-associated fungus sporidesmium circinophorum kufa 0043 ... | 2016 | 27054912 |
| the type iii secretion system apparatus determines the intracellular niche of bacterial pathogens. | upon entry into host cells, intracellular bacterial pathogens establish a variety of replicative niches. although some remodel phagosomes, others rapidly escape into the cytosol of infected cells. little is currently known regarding how professional intracytoplasmic pathogens, including shigella, mediate phagosomal escape. shigella, like many other gram-negative bacterial pathogens, uses a type iii secretion system to deliver multiple proteins, referred to as effectors, into host cells. here, us ... | 2016 | 27078095 |
| in search of alternative antibiotic drugs: quorum-quenching activity in sponges and their bacterial isolates. | owing to the extensive development of drug resistance in pathogens against the available antibiotic arsenal, antimicrobial resistance is now an emerging major threat to public healthcare. anti-virulence drugs are a new type of therapeutic agent aiming at virulence factors rather than killing the pathogen, thus providing less selective pressure for evolution of resistance. one promising example of this therapeutic concept targets bacterial quorum sensing (qs), because qs controls many virulence f ... | 2016 | 27092109 |
| a β-alanine catabolism pathway containing a highly promiscuous ω-transaminase in the 12-aminododecanate-degrading pseudomonas sp. strain aac. | we previously isolated the transaminase kes23458 from pseudomonas sp. strain aac as a promising biocatalyst for the production of 12-aminododecanoic acid, a constituent building block of nylon-12. here, we report the subsequent characterization of this transaminase. it exhibits activity with a broad substrate range which includes α-, β-, and ω-amino acids, as well as α,ω-diamines and a number of other industrially relevant compounds. it is therefore a prospective candidate for the biosynthesis o ... | 2016 | 27107110 |
| genetic functions of the naip family of inflammasome receptors for bacterial ligands in mice. | biochemical studies suggest that the naip family of nlr proteins are cytosolic innate receptors that directly recognize bacterial ligands and trigger nlrc4 inflammasome activation. in this study, we generated naip5(-/-), naip1(-/-), and naip2(-/-) mice and showed that bone marrow macrophages derived from these knockout mice are specifically deficient in detecting bacterial flagellin, the type iii secretion system needle, and the rod protein, respectively. naip1(-/-), naip2(-/-), and naip5(-/-) m ... | 2016 | 27114610 |
| antioxidant and anti-quorum sensing potential of acer monspessulanum subsp. monspessulanum extracts. | in this study, anti-quorum sensing, and antioxidant activities, and chemical composition of acer monspessulanum subsp. monspessulanum extracts were evaluated. determination of the antioxidant activity was revealed by dpph radical scavenging activity, the total phenolic content assay, and the β-carotene/linoleic acid assay. the detection of phenolic compounds was determined using rp-hplc. anti-quorum sensing activity and violacein inhibition activity were determined using chromobacterium violaceu ... | 2016 | 27116707 |
| dna methylation and chromatin remodeling: the blueprint of cancer epigenetics. | epigenetics deals with the interactions between genes and the immediate cellular environment. these interactions go a long way in shaping up each and every person's individuality. further, reversibility of epigenetic interactions may offer a dynamic control over the expression of various critical genes. thus, tweaking the epigenetic machinery may help cause or cure diseases, especially cancer. therefore, cancer epigenetics, especially at a molecular level, needs to be scrutinised closely, as it ... | 2016 | 27119045 |
| identification of a new polyhydroxyalkanoate (pha) producer aquitalea sp. usm4 (jcm 19919) and characterization of its pha synthase. | aquitalea sp. usm4 (jcm 19919) was isolated from a freshwater sample at lata iskandar waterfall in perak, malaysia. it is a rod-shaped, gram-negative bacterium with high sequence identity (99%) to aquitalea magnusonii based on 16s rrna gene analysis. aquitalea sp. usm4 also possessed a pha synthase gene (phac), which had amino acid sequence identity of 77-78% to the pha synthase of chromobacterium violaceum atcc12472 and pseudogulbenkiania sp. nh8b. pha biosynthesis results showed that wild-type ... | 2016 | 27132174 |
| protist-bacteria associations: gammaproteobacteria and alphaproteobacteria are prevalent as digestion-resistant bacteria in ciliated protozoa. | protistan bacterivory, a microbial process involving ingestion and digestion, is ecologically important in the microbial loop in aquatic and terrestrial ecosystems. while bacterial resistance to protistan ingestion has been relatively well understood, little is known about protistan digestion in which some ingested bacteria could not be digested in cells of major protistan grazers in the natural environment. here we report the phylogenetic identities of digestion-resistant bacteria (drb) that co ... | 2016 | 27148188 |
| cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen. | quorum sensing (qs) is the process of population dependent cell to cell communication used by bacteria to regulate their phenotypic characteristics. key virulence factors that determine the bacterial pathogenicity and food spoilage were found to be regulated by qs mechanism. hence, disrupting the qs signaling pathway could be an attractive strategy to manage food borne pathogens. in the current study, qs inhibitory activity of a naturally occurring anthocyanin-cyanidin and its anti-biofilm prope ... | 2016 | 27162376 |
| genetic variability in isolates of chromobacterium violaceum from pulmonary secretion, water, and soil. | chromobacterium violaceum is a free-living gram-negative bacillus usually found in the water and soil in tropical regions, which causes infections in humans. chromobacteriosis is characterized by rapid dissemination and high mortality. the aim of this study was to detect the genetic variability among c. violaceum type strain atcc 12472, and seven isolates from the environment and one from a pulmonary secretion from a chromobacteriosis patient from ilhéus, bahia. the molecular characterization of ... | 2016 | 27173292 |
| quorum sensing control of type vi secretion factors restricts the proliferation of quorum-sensing mutants. | burkholderia thailandensis uses acyl-homoserine lactone-mediated quorum sensing systems to regulate hundreds of genes. here we show that cell-cell contact-dependent type vi secretion (t6s) toxin-immunity systems are among those activated by quorum sensing in b. thailandensis. we also demonstrate that t6s is required to constrain proliferation of quorum sensing mutants in colony cocultures of a btar1 quorum-sensing signal receptor mutant and its parent. however, the btar1 mutant is not constraine ... | 2016 | 27183270 |
| the draft genome sequence of the yersinia entomophaga entomopathogenic type strain mh96t. | here we report the draft genome of yersinia entomophaga type strain mh96t. the genome shows 93.8% nucleotide sequence identity to that of yersinia nurmii type strain apn3a-ct, and comprises a single chromosome of approximately 4,275,531 bp. in silico analysis identified that, in addition to the previously documented y. entomophaga yen-tc gene cluster, the genome encodes a diverse array of toxins, including two type iii secretion systems, and five rhs-associated gene clusters. as well as these mu ... | 2016 | 27187466 |
| broad spectrum anti-quorum sensing activity of tannin-rich crude extracts of indian medicinal plants. | quorum sensing (qs) mechanisms have been demonstrated to have significance in expression of pathogenicity in infectious bacteria. in gram negative bacteria the autoinducer molecules that mediate qs are acyl homoserine lactones (ahl) and in gram positive bacteria they are peptides called autoinducing peptides (aip). a screening of tannin-rich medicinal plants was attempted to identify extracts that could interrupt the qs mechanisms in both gram positive and gram negative bacteria over a wide rang ... | 2016 | 27190686 |
| an innovative method for rapid identification and detection of vibrio alginolyticus in different infection models. | vibrio alginolyticus is one of the most common pathogenic marine vibrio species, and has been found to cause serious seafood-poisoning or fatal extra-intestinal infections in humans, such as necrotizing soft-tissue infections, bacteremia, septic shock, and multiple organ failures. delayed accurate diagnosis and treatment of most vibrio infections usually result to high mortality rates. the objective of this study was to establish a rapid diagnostic method to detect and identify the presence of v ... | 2016 | 27199971 |
| fast, continuous, and high-throughput (bio)chemical activity assay for n-acyl-l-homoserine lactone quorum-quenching enzymes. | quorum sensing, the bacterial cell-cell communication by small molecules, controls important processes such as infection and biofilm formation. therefore, it is a promising target with several therapeutic and technical applications besides its significant ecological relevance. enzymes inactivating n-acyl-l-homoserine lactones, the most common class of communication molecules among gram-negative proteobacteria, mainly belong to the groups of quorum-quenching lactonases or quorum-quenching acylase ... | 2016 | 27208131 |
| the brief case: safe to go back in the water? vibrio parahaemolyticus wound infection associated with brackish water. | 2016 | 27217385 | |
| diarylheptanoids from alnus viridis ssp. viridis and alnus glutinosa: modulation of quorum sensing activity in pseudomonas aeruginosa. | diarylheptanoids from the barks of alnus viridis ssp. viridis (green alder) and alnus glutinosa (black alder) were explored for anti-quorum sensing activity. chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. the anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely pseudomonas aeruginosa pao1 and chromobacterium violaceum cv026. although all of the investigated compounds negatively influence ... | 2017 | 27220074 |
| gqqa, a novel protein in komagataeibacter europaeus involved in bacterial quorum quenching and cellulose formation. | we report on the functional screening and identification of an active quorum quenching (qq) gene in the komagataeibacter europaeus strain cect 8546, which is a member of the acetic acid bacteria (aab). | 2016 | 27221658 |
| maldi-tof ms contribution to diagnosis of melioidosis in a nonendemic country in three french travellers. | melioidosis is an endemic disease in southeast asia and northern australia. an increasing number of cases are being reported in nonendemic countries, making the diagnosis less obvious. we discuss the identification of burkholderia pseudomallei using matrix-assisted desorption ionization-time of flight mass spectrometry on the occasion of recent cases of imported melioidosis in french travellers. | 2016 | 27222715 |
| clinical use and applications of histone deacetylase inhibitors in multiple myeloma. | the incorporation of various novel therapies has resulted in a significant survival benefit in newly diagnosed and relapsed patients with multiple myeloma (mm) over the past decade. despite these advances, resistance to therapy leads to eventual relapse and fatal outcomes in the vast majority of patients. hence, there is an unmet need for new safe and efficacious therapies for continued improvement in outcomes. given the role of epigenetic aberrations in the pathogenesis and progression of mm an ... | 2016 | 27226735 |
| inhibition of nuclear transport of nf-ĸb p65 by the salmonella type iii secretion system effector spvd. | salmonella enterica replicates in macrophages through the action of effector proteins translocated across the vacuolar membrane by a type iii secretion system (t3ss). here we show that the spi-2 t3ss effector spvd suppresses proinflammatory immune responses. spvd prevented activation of an nf-ĸb-dependent promoter and caused nuclear accumulation of importin-α, which is required for nuclear import of p65. spvd interacted specifically with the exportin xpo2, which mediates nuclear-cytoplasmic recy ... | 2016 | 27232334 |
| non-thermal plasma exposure rapidly attenuates bacterial ahl-dependent quorum sensing and virulence. | the antimicrobial activity of atmospheric pressure non-thermal plasma has been exhaustively characterised, however elucidation of the interactions between biomolecules produced and utilised by bacteria and short plasma exposures are required for optimisation and clinical translation of cold plasma technology. this study characterizes the effects of non-thermal plasma exposure on acyl homoserine lactone (ahl)-dependent quorum sensing (qs). plasma exposure of ahls reduced the ability of such molec ... | 2016 | 27242335 |
| selection of the n-acylhomoserine lactone-degrading bacterium alteromonas stellipolaris pqq-42 and of its potential for biocontrol in aquaculture. | the production of virulence factors by many pathogenic microorganisms depends on the intercellular communication system called quorum sensing, which involves the production and release of signal molecules known as autoinducers. based on this, new-therapeutic strategies have emerged for the treatment of a variety of infections, such as the enzymatic degradation of signaling molecules, known as quorum quenching (qq). in this study, we present the screening of qq activity amongst 450 strains isolat ... | 2016 | 27242684 |
| quorum sensing inhibiting activity of streptomyces coelicoflavus isolated from soil. | quorum sensing (qs) systems communicate bacterial population and stimulate microbial pathogenesis through signaling molecules. inhibition of qs signals potentially suppresses microbial infections. antimicrobial properties of streptomyces have been extensively studied, however, less is known about quorum sensing inhibitory (qsi) activities of streptomyces. this study explored the qsi potential of streptomyces isolated from soil. sixty-five bacterial isolates were purified from soil samples with m ... | 2016 | 27242690 |
| bacteriological quality of bottled drinking water versus municipal tap water in dharan municipality, nepal. | water-related diseases are of great concern in developing countries like nepal. every year, there are countless morbidity and mortality due to the consumption of unsafe drinking water. recently, there have been increased uses of bottled drinking water in an assumption that the bottled water is safer than the tap water and its use will help to protect from water-related diseases. so, the main objective of this study was to analyze the bacteriological quality of bottled drinking water and that of ... | 2016 | 27267213 |