Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a unique bicyclic monosaccharide from the bradyrhizobium lipopolysaccharide and its role in the molecular interaction with plants. | sugar coat: the nitrogen-fixing soil bacterium bradyrhizobium sp. btai1 is coated with a unique lipopolysaccharide that does not induce innate immune responses in its host plant aeschynomene indica or in different plant families. the chemical nature of the monosaccharide forming the polymer is unprecedented in nature, which helps to avoid "harmful" recognition by its symbiotic host. | 2011 | 22058060 |
| Evolutionary instability of symbiotic function in Bradyrhizobium japonicum. | Bacterial mutualists are often acquired from the environment by eukaryotic hosts. However, both theory and empirical work suggest that this bacterial lifestyle is evolutionarily unstable. Bacterial evolution outside of the host is predicted to favor traits that promote an independent lifestyle in the environment at a cost to symbiotic function. Consistent with these predictions, environmentally-acquired bacterial mutualists often lose symbiotic function over evolutionary time. Here, we investiga ... | 2011 | 22073160 |
| Development and application of a multilocus sequence analysis method for the identification of genotypes within the genus Bradyrhizobium and for establishing nodule occupancy of soybean (Glycine max L. Merr). | A Multilocus Sequence Typing (MLST) method based on allelic variation of 7 chromosomal loci was developed for characterizing genotypes within the genus Bradyrhizobium. With the method 29 distinct multilocus genotypes (GTs) were identified among 190 culture collection soybean strains. The occupancy of 347 nodules taken from uninoculated field-grown soybean plants also was determined. The bacteroid genotypes were either the same or were closely related to genotypes identified among strains in th ... | 2011 | 22074348 |
| Efficient electricity generation from sewage sludge usingbiocathode microbial fuel cell. | Microbial fuel cells (MFCs) with abiotic cathodes require expensive catalyst (such as Pt) or catholyte (such as hexacynoferrate) to facilitate oxidation reactions. This study incorporated biocathodes into a three-chamber MFC to yield electricity from sewage sludge at maximum power output of 13.2±1.7W/m(3) during polarization, much higher than those previously reported. After 15d operation, the total chemical oxygen demand (TCOD) removal and coulombic efficiency (CE) of cell reached 40.8±9.0% and ... | 2012 | 22078254 |
| nitrogen fixation and nitrogen fertilization of soybeans. | abstract in pot experiments with (15)n labelled soil and mineral (15)n, the influence of bradyrhizobium (rhizobium japonicum) inoculation and n fertilization on the symbiotic n(2) fixation and yield of soybeans [glycine max (l.) merill., cv. 'fiskeby v'] was investigated. symbiotic n(2) fixation only occured after inoculation with bradyrhizobium. considerable differences in efficiency of the bacterial preparations were observed. shortly after flowering, the symbiotic nitrogen fixation was fini ... | 1996 | 22088108 |
| phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes. | lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ... | 2011 | 22092879 |
| the nitric oxide response in plant-associated endosymbiotic bacteria. | nitric oxide (no) is a gaseous signalling molecule which becomes very toxic due to its ability to react with multiple cellular targets in biological systems. bacterial cells protect against no through the expression of enzymes that detoxify this molecule by oxidizing it to nitrate or reducing it to nitrous oxide or ammonia. these enzymes are haemoglobins, c-type nitric oxide reductase, flavorubredoxins and the cytochrome c respiratory nitrite reductase. expression of the genes encoding these enz ... | 2011 | 22103544 |
| Denitrification in Sinorhizobium meliloti. | Denitrification is the complete reduction of nitrate or nitrite to N2, via the intermediates nitric oxide (NO) and nitrous oxide (N2O), and is coupled to energy conservation and growth under O2-limiting conditions. In Bradyrhizobium japonicum, this process occurs through the action of the napEDABC, nirK, norCBQD and nosRZDFYLX gene products. DNA sequences showing homology with nap, nirK, nor and nos genes have been found in the genome of the symbiotic plasmid pSymA of Sinorhizobium meliloti stra ... | 2011 | 22103545 |
| Biodegradation of melamine and its hydroxy derivatives by a bacterial consortium containing a novel Nocardioides species. | Melamine has recently been recognized as a food contaminant with adverse human health effects. Melamine contamination in some crops arises from soil and water pollution from various causes. To remove melamine from the polluted environment, a novel bacterium, Nocardioides sp. strain ATD6, capable of degrading melamine was enriched and isolated from a paddy soil sample. The enrichment culture was performed by the soil-charcoal perfusion method in the presence of triazine-degrading bacteria previou ... | 2011 | 22105542 |
| change in land use alters the diversity and composition of bradyrhizobium communities and led to the introduction of rhizobium etli into the tropical rain forest of los tuxtlas (mexico). | nitrogen-fixing bacteria of the bradyrhizobium genus are major symbionts of legume plants in american tropical forests, but little is known about the effects of deforestation and change in land use on their diversity and community structure. forest clearing is followed by cropping of bean (phaseolus vulgaris) and maize as intercropped plants in los tuxtlas tropical forest of mexico. the identity of bean-nodulating rhizobia in this area is not known. using promiscuous trap plants, bradyrhizobia w ... | 2011 | 22109095 |
| functional characterization of nopt1 and nopt2, two type iii effectors of bradyrhizobium japonicum. | nopt1 and nopt2, putative type iii effectors from the plant symbiotic bacterium bradyrhizobium japonicum, are predicted to belong to a family of yopt/avrpphb effectors, which are cysteine proteases. in the present study, we showed that both nopt1 and nopt2 indeed possess cysteine protease activity. when overexpressed in escherichia coli, both nopt1 and nopt2 undergo autoproteolytic processing which is largely abolished in the presence of e-64, a papain family-specific inhibitor. mutations of nop ... | 2011 | 22112296 |
| proteomics reveals differential expression of proteins related to a variety of metabolic pathways by genistein-induced bradyrhizobium japonicum strains. | the rhizobia-legume symbiosis requires a coordinated molecular interaction between the symbionts, initiated by seed and root exudation of several compounds, mainly flavonoids, that trigger the expression of nodulation genes in the bacteria. since the role of flavonoids seems to be broader than the induction of nodulation genes, we aimed at characterizing genistein-induced proteins of bradyrhizobium japonicum cpac 15 (=semia 5079), used in commercial soybean inoculants in brazil, and of two genet ... | 2011 | 22119543 |
| effect of rj-genotype and cultivation temperature on the community structure of soybean-nodulating bradyrhizobia. | the nodulation tendency and community structure of indigenous bradyrhizobia on rj-genotype soybean cultivars at cultivation temperatures of 33/28°c, 28/23°c and 23/18°c, 16/8 h (day/night degrees, hours) were investigated using 780 bradyrhizobial dna samples from an andosol with 13 soybean cultivars of four rj-genotypes (non-rj, rj(2)rj(3), rj(4) and rj(2)rj(3)rj(4)). a dendrogram was constructed based on restriction fragment length polymorphism of the polymerase chain reaction products (pcr-rfl ... | 2011 | 22156423 |
| long-term field fertilization alters the diversity of autotrophic bacteria based on the ribulose-1,5-biphosphate carboxylase/oxygenase (rubisco) large-subunit genes in paddy soil. | carbon dioxide (co(2)) assimilation by autotrophic bacteria is an important process in the soil carbon cycle with major environmental implications. the long-term impact of fertilizer on co(2) assimilation in the bacterial community of paddy soils remains poorly understood. to narrow this knowledge gap, the composition and abundance of co(2)-assimilating bacteria were investigated using terminal restriction fragment length polymorphism and quantitative pcr of the cbbl gene [that encodes ribulose- ... | 2011 | 22159889 |
| Phylogenetic diversity and symbiotic functioning in mungbean (Vigna radiata L. Wilczek) bradyrhizobia from contrast agro-ecological regions of Nepal. | Nepal consists wide range of climatic and topographical variations. Here, we explored the phylogeny of native mungbean bradyrhizobia isolated from different agro-ecological regions of Nepal and accessed their nodulation and nitrogen fixation characteristics. Soil samples were collected from three agro-ecological regions with contrasting climate and topography. A local mungbean cultivar, Kalyan, was used as a trap plant. We characterized isolates based on the full nucleotide sequence of the 16S r ... | 2012 | 22178390 |
| microvirga lupini sp. nov., microvirga lotononidis sp. nov., and microvirga zambiensis sp. nov. are alphaproteobacterial root nodule bacteria that specifically nodulate and fix nitrogen with geographically and taxonomically separate legume hosts. | strains of gram-negative, rod-shaped, non-spore-forming bacteria were isolated from nitrogen-fixing nodules of the native legumes listia angolensis (from zambia) and lupinus texensis (from texas, usa). phylogenetic analysis of the 16s rrna gene showed that the novel strains belong to the genus microvirga, with 96.1 % or greater sequence similarity with type strains of this genus. the closest relative of the representative strains lut6t and wsm3557t was m. flocculans tfbt, with 97.6-98.0 % simila ... | 2011 | 22199210 |
| Unique structural features and sequence motifs of proline utilization A (PutA). | Proline utilization A proteins (PutAs) are bifunctional enzymes that catalyze the oxidation of proline to glutamate using spatially separated proline dehydrogenase and pyrroline-5-carboxylate dehydrogenase active sites. Here we use the crystal structure of the minimalist PutA from Bradyrhizobium japonicum (BjPutA) along with sequence analysis to identify unique structural features of PutAs. This analysis shows that PutAs have secondary structural elements and domains not found in the related mon ... | 2012 | 22201760 |
| [genetic diversity and phylogeny of bradyrhizobia associated with desmodium spp]. | genetic diversity and phylogeny of bradyrhizobial strains associated with desmodium spp. in subtropic and temperate regions of china were analyzed. | 2011 | 22233051 |
| mercury-resistant rhizobial bacteria isolated from nodules of leguminous plants growing in high hg-contaminated soils. | a survey of symbiotic bacteria from legumes grown in high mercury-contaminated soils (almadén, spain) was performed to produce a collection of rhizobia which could be well adapted to the environmental conditions of this region and be used for restoration practices. nineteen hg-tolerant rhizobia were isolated from nodules of 11 legume species (of the genera medicago, trifolium, vicia, lupinus, phaseolus, and retama) and characterized. based on their growth on hg-supplemented media, the isolates w ... | 2012 | 22246528 |