Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the influence of micronutrients on oral and general health. | the aim of the present clinical pilot study was to examine the influence of a combination of micronutrients on individuals with high stress experience. | 2011 | 22027646 |
| evaluation of two mouth rinses in reduction of oral malodor using a spectrophotometric technique. | various mouth rinses have been used in the treatment of halitosis, but most of the evidence for the efficacy of mouth rinses is anecdotal. in the present study, 0.2% chlorhexidine rinse and an essential oil mouth rinse are compared for their efficacy in reducing the breath mercaptan levels. | 2011 | 22028512 |
| Protease inhibitor levels in periodontal health and disease. | Kretschmar S, Yin L, Roberts F, London R, Flemmig TT, Arushanov D, Kaiyala K, Chung WO. Protease inhibitor levels in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01425.x. © 2011 John Wiley & Sons A/S Background and Objective: Our previous study showed that protease inhibitors were attenuated by the periodontal pathogen Porphyromonas gingivalis in cultured gingival epithelial cells. We hypothesize that fewer protease inhibitors would be present in more adva ... | 2011 | 22029638 |
| Protease inhibitor levels in periodontal health and disease. | Kretschmar S, Yin L, Roberts F, London R, Flemmig TT, Arushanov D, Kaiyala K, Chung WO. Protease inhibitor levels in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01425.x. © 2011 John Wiley & Sons A/S Background and Objective: Our previous study showed that protease inhibitors were attenuated by the periodontal pathogen Porphyromonas gingivalis in cultured gingival epithelial cells. We hypothesize that fewer protease inhibitors would be present in more adva ... | 2011 | 22029638 |
| Filifactor alocis interactions with gingival epithelial cells. | An association between the gram-positive anaerobe Filifactor alocis and periodontal disease has recently emerged; however, possible pathogenic mechanisms have not been investigated. In this study we examined the responses of primary cultures of gingival epithelial cells (GECs) to infection with F. alocis. Secretion of the pro-inflammatory cytokines interleukin-1ß, interleukin-6 and tumor necrosis factor-a from GECs was stimulated by F. alocis infection. F. alocis also induced apoptosis in GECs t ... | 2011 | 22053964 |
| clinical and microbiological evaluation, by real-time pcr, of non-surgical treatment of aggressive periodontitis associated with amoxicillin and metronidazole. | background: to evaluate the clinical and microbiological changes resulting from non-surgical periodontal treatment associated with amoxicillin and metronidazole, in individuals with aggressive periodontitis (agp). methods: 15 subjects with agp received non-surgical periodontal treatment and 45 days after completion of treatment, were treated with antibiotics. clinical data and samples of subgingival plaque were collected at baseline, 45 days after the non-surgical periodontal treatment and 1 mon ... | 2011 | 22060046 |
| Highly Specific Protease-Based Approach for Detection of Porphyromonas gingivalis in Diagnosis of Periodontitis. | Porphyromonas gingivalis is associated with the development of periodontitis. Here we describe the development of a highly specific protease-based diagnostic method for the detection of P. gingivalis in gingival crevicular fluid. Screening of a proteolytic peptide substrate library, including fluorogenic dipeptides that contain d-amino acids, led to the discovery of five P. gingivalis-specific substrates. Due to the presence of lysine and arginine residues in these substrates, it was hypothesize ... | 2012 | 22075590 |
| Associations of periodontal microorganisms with salivary proteins and MMP-8 in gingival crevicular fluid. | OBJECTIVE: We investigated in subjects with and without periodontitis, the levels of certain salivary proteins and matrix metalloproteinase-8 (MMP-8) in gingival crevicular fluid (GCF), in relation to the presence of specific periodontal pathogens. METHODS: Clinical parameters were recorded at baseline, in 1985 and in 2009 from 99 subjects; 56 with and 43 without periodontitis (mean age 59.2 ± SD 2.9). Saliva samples collected in 2009 were analysed for salivary albumin, total protein and immunog ... | 2011 | 22103335 |
| Treatment of residual pockets with photodynamic therapy, diode laser, or deep scaling. A randomized, split-mouth controlled clinical trial. | The objective of this work was to compare the effects of antimicrobial photodynamic therapy (PDT), diode soft laser therapy (DSL), and thorough deep scaling and root planing (SRP) for treatment of residual pockets. Thirty-two subjects with a history of non-surgical treatment for chronic periodontitis were included. Residual pockets >4 mm and bleeding upon probing were debrided with an ultrasonic device and then subjected to either PDT, DSL, or SRP. Pocket probing depth (PPD), bleeding on probing ... | 2011 | 22105837 |
| tobacco smoke augments porphyromonas gingivalis-streptococcus gordonii biofilm formation. | smoking is responsible for the majority of periodontitis cases in the us and smokers are more susceptible than non-smokers to infection by the periodontal pathogen porphyromonas gingivalis. p. gingivalis colonization of the oral cavity is dependent upon its interaction with other plaque bacteria, including streptococcus gordonii. microarray analysis suggested that exposure of p. gingivalis to cigarette smoke extract (cse) increased the expression of the major fimbrial antigen (fima), but not the ... | 2011 | 22110637 |
| Porphyromonas gingivalis: a clonal pathogen?: Diversities in housekeeping genes and the major fimbriae gene. | The introduction of multilocus sequence typing (MLST) in infectious disease research has allowed standardized typing of bacterial clones. Through multiple markers around the genome, it is possible to determine the sequence type (ST) of bacterial isolates to establish the population structure of a species. For the periodontal pathogen, Porphyromonas gingivalis, the MLST scheme has been established at www.pubmlst.org/pgingivalis, and data from the database indicate a high degree of genetic diversi ... | 2011 | 22125739 |
| relationship between periodontal diseases and preterm birth: recent epidemiological and biological data. | for ten years, the incidence of preterm birth does not decrease in developed countries despite the promotion of public health programs. many risk factors have been identified including ethnicity, age, tobacco, and infection. however, almost 50% of preterm birth causes remain unknown. the periodontal diseases are highly prevalent inflammatory and infectious diseases of tooth supporting tissues leading to an oral disability. they influence negatively general health worsening cardiovascular disease ... | 2011 | 22132334 |
| a machine-learning approach to identify hydrogenosomal proteins in trichomonas vaginalis. | the protozoan parasite trichomonas vaginalis is the causative agent of trichomoniasis, the most widespread non-viral sexually transmitted disease in humans. it possesses hydrogenosomes - anaerobic mitochondria that generate h(2), co(2), and acetate from pyruvate while converting adp to atp via substrate level phosphorylation. t. vaginalis hydrogenosomes lack a genome and translation machinery, hence they import all their proteins from the cytosol, but to date only 30 imported proteins have been ... | 2011 | 22140228 |
| neutrophils in periodontal inflammation. | neutrophils (also called polymorphonuclear leukocytes) are the most abundant leukocytes whose primary purpose as anti-microbial professional phagocytes is to kill extracellular pathogens. neutrophils and macrophages are phagocytic cell types that along with other cells effectively link the innate and adaptive arms of the immune response, and help promote inflammatory resolution and tissue healing. found extensively within the gingival crevice and epithelium, neutrophils are considered the key pr ... | 2011 | 22142957 |
| neutrophils in periodontal inflammation. | neutrophils (also called polymorphonuclear leukocytes) are the most abundant leukocytes whose primary purpose as anti-microbial professional phagocytes is to kill extracellular pathogens. neutrophils and macrophages are phagocytic cell types that along with other cells effectively link the innate and adaptive arms of the immune response, and help promote inflammatory resolution and tissue healing. found extensively within the gingival crevice and epithelium, neutrophils are considered the key pr ... | 2011 | 22142957 |
| animal models to study host-bacteria interactions involved in periodontitis. | animal models have distinct advantages because they can mimic cellular complexities that occur in humans in vivo and are often more accurate than in vitro studies that take place on plastic surfaces with limited numbers of cell types present. furthermore, cause and effect relationships can be established by applying inhibitors or activators or through the use of genetically modified animals. such gain or loss of function studies are often difficult to achieve in human clinical studies, particula ... | 2012 | 22142960 |
| symptomatic and asymptomatic apical periodontitis associated with red complex bacteria: clinical and microbiological evaluation. | in this study, the association of red complex (rc) bacteria that include treponema denticola, tannerella forsythia and porphyromonas gingivalis with acute, exacerbated or chronic apical periodontitis was evaluated. seventy-one patients with periapical disease were evaluated by clinical examination and microbiological samples obtained from the root canals were analyzed by a polymerase chain reaction assay. twenty-one (29.6%) samples were positive for rc bacteria, with t. ... | 2011 | 22143381 |
| papillon-lefévre syndrome. | the goal of periodontal therapy has always been regeneration of the lost tissues. however, conventional periodontal therapy has not always been successful in achieving regeneration, especially when it is part of a syndrome. this case report involves a 13-year old male patient with the chief complaint of mobile teeth for over 3 months. his dental history revealed early loss of primary dentition, around 3-4 years of age and that he noticed mobility of permanent incisors and molars at 9-10 years. k ... | 2011 | 22144846 |
| [Role of several periodontopathogenic microorganisms and tlr4 gene Asp299Gly polymorphism in atherosclerosis pathogenesis]. | Establishment of presence of periodonto-pathogenic microorganisms in atherosclerosis plaque and surrounding tissues, and possible relation of development of atherosclerosis and TLR4 gene Asp299Gly polymorphism in ischemic heart disease patients (IHD). | 2011 | 22145357 |
| immune evasion strategies of porphyromonas gingivalis. | porphyromonas gingivalis is strongly correlated with chronic periodontitis. its chronic persistence in the periodontium depends on its ability to evade host immunity without inhibiting the overall inflammatory response, which is actually beneficial for this and other periodontal bacteria. indeed, the inflammatory exudate (gingival crevicular fluid) is a source of essential nutrients, such as peptides and hemin-derived iron. in this review, i discuss how p. gingivalis can promote its adaptive fit ... | 2011 | 22162663 |
| correlation network analysis applied to complex biofilm communities. | the complexity of the human microbiome makes it difficult to reveal organizational principles of the community and even more challenging to generate testable hypotheses. it has been suggested that in the gut microbiome species such as bacteroides thetaiotaomicron are keystone in maintaining the stability and functional adaptability of the microbial community. in this study, we investigate the interspecies associations in a complex microbial biofilm applying systems biology principles. using corr ... | 2011 | 22163302 |
| distinct and complex bacterial profiles in human periodontitis and health revealed by 16s pyrosequencing. | periodontitis has a polymicrobial etiology within the framework of a complex microbial ecosystem. with advances in sequencing technologies, comprehensive studies to elucidate bacterial community differences have recently become possible. we used 454 sequencing of 16s rrna genes to compare subgingival bacterial communities from 29 periodontally healthy controls and 29 subjects with chronic periodontitis. amplicons from both the v1-2 and v4 regions of the 16s gene were sequenced, yielding 1 393 57 ... | 2011 | 22170420 |
| distinct and complex bacterial profiles in human periodontitis and health revealed by 16s pyrosequencing. | periodontitis has a polymicrobial etiology within the framework of a complex microbial ecosystem. with advances in sequencing technologies, comprehensive studies to elucidate bacterial community differences have recently become possible. we used 454 sequencing of 16s rrna genes to compare subgingival bacterial communities from 29 periodontally healthy controls and 29 subjects with chronic periodontitis. amplicons from both the v1-2 and v4 regions of the 16s gene were sequenced, yielding 1 393 57 ... | 2011 | 22170420 |
| A New Real-Time PCR Assay for Improved Detection of the Parasite Babesia microti. | Babesiosis is an emerging zoonosis with important public health implications, as the incidence of the disease has risen dramatically over the past decade. Because the current gold standard for detection of Babesia is microscopic examination of blood smears, accurate identification requires trained personnel. Species in the genus cannot be distinguished microscopically, and Babesia can also be confused with the early trophozoite stage (ring forms) of Plasmodium parasites. To allow more accurate d ... | 2011 | 22170915 |
| Identification of iron-regulated genes of Bifidobacterium breve UCC2003 as a basis for controlled gene expression. | Iron is an essential growth factor for virtually all organisms. However, iron is not readily available in most environments and microorganisms have evolved specialized mechanisms, such as the use of siderophores and high-affinity transport systems, to acquire iron when confronted with iron-limiting conditions. In general these systems are tightly regulated to prevent iron-induced toxicity and because they are quite costly to the microbe. Because of this tight regulation we chose to explore the r ... | 2012 | 22179149 |
| relationships among interleukin-6, tumor necrosis factor-α, adipokines, vitamin d, and chronic periodontitis. | the aim of this study is to explore relationships among serum adipokines, vitamin d, and clinical and microbial parameters of chronic periodontitis before and after treatment. | 2011 | 22181684 |
| relationships among interleukin-6, tumor necrosis factor-α, adipokines, vitamin d, and chronic periodontitis. | the aim of this study is to explore relationships among serum adipokines, vitamin d, and clinical and microbial parameters of chronic periodontitis before and after treatment. | 2011 | 22181684 |
| the antibacterial activity of ll-37 against treponema denticola is dentilisin-protease independent and facilitated by the major outer sheath protein virulence factor. | host defense peptides are innate immunoeffectors that possess both bactericidal activities and immunomodulatory functions. deficiency in the human ll-37 host defense peptide has been previously correlated with severe periodontal disease. treponema denticola is an oral anaerobic spirochete closely associated with the pathogenesis of periodontal disease. the t. denticola major surface protein (msp), involved in adhesion and in cytotoxicity, and the dentilisin serine protease are key virulence fact ... | 2011 | 22184422 |
| bacterial invasion of vascular cell types: vascular infectology and atherogenesis. | to portray the chronic inflammation in atherosclerosis, leukocytic cell types involved in the immune response to invading pathogens are often the focus. however, atherogenesis is a complex pathological deterioration of the arterial walls, where vascular cell types are participants with regards to deterioration and disease. since other recent reviews have detailed the role of both the innate and adaptive immune response in atherosclerosis, herein we will summarize the latest developments regardin ... | 2012 | 22185451 |
| bioinformatic characterization of the 4-toluene sulfonate uptake permease (tsup) family of transmembrane proteins. | the ubiquitous sequence diverse 4-toluene sulfonate uptake permease (tsup) family contains few characterized members and is believed to catalyze the transport of several sulfur-based compounds. prokaryotic members of the tsup family outnumber the eukaryotic members substantially, and in prokaryotes, but not eukaryotes, extensive lateral gene transfer occurred during family evolution. despite unequal representation, homologues from the three taxonomic domains of life share well-conserved motifs. ... | 2012 | 22192777 |
| bioinformatic characterization of the 4-toluene sulfonate uptake permease (tsup) family of transmembrane proteins. | the ubiquitous sequence diverse 4-toluene sulfonate uptake permease (tsup) family contains few characterized members and is believed to catalyze the transport of several sulfur-based compounds. prokaryotic members of the tsup family outnumber the eukaryotic members substantially, and in prokaryotes, but not eukaryotes, extensive lateral gene transfer occurred during family evolution. despite unequal representation, homologues from the three taxonomic domains of life share well-conserved motifs. ... | 2012 | 22192777 |
| human microbiome and hiv/aids. | understanding of the human microbiome continues to grow rapidly; however, reports on changes in the microbiome after hiv infection are still limited. this review surveys the progress made in methodology associated with microbiome studies and highlights the remaining challenges to this field. studies have shown that commensal oral, gut, vaginal, and penile bacteria are vital to the health of the human immune system. our studies on crosstalk among oral and gastrointestinal soluble innate factors, ... | 2011 | 22193889 |
| molecular detection of in-vivo microbial contamination of metallic orthodontic brackets by checkerboard dna-dna hybridization. | knowing the microbiota that colonizes orthodontic appliances is important for planning strategies and implementing specific preventive measures during treatment. the purpose of this clinical trial was to evaluate in vivo the contamination of metallic orthodontic brackets with 40 dna probes for different bacterial species by using the checkerboard dna-dna hybridization (cddh) technique. | 2012 | 22196182 |
| evidence for graft colonization with periodontal pathogens in lung transplant recipients. | summary bronchiolitis obliterans syndrome (bos) is a major cause of late graft dysfunction in lung transplant recipients. there is increasing evidence that beside alloimmunologic injury also non-alloimmunologic inflammatory conditions may raise the risk of acute and chronic rejection. the oral cavity represents a possible reservoir for pathogenic bacteria due to its close anatomical proximity. in this pilot study, the presence of pathogenic periodontal bacteria in the oral cavity as well as in t ... | 2011 | 22203528 |
| identification of surprisingly diverse type iv pili, across a broad range of gram-positive bacteria. | in gram-negative bacteria, type iv pili (tfp) have long been known to play important roles in such diverse biological phenomena as surface adhesion, motility, and dna transfer, with significant consequences for pathogenicity. more recently it became apparent that gram-positive bacteria also express type iv pili; however, little is known about the diversity and abundance of these structures in gram-positives. computational tools for automated identification of type iv pilins are not currently ava ... | 2011 | 22216142 |
| full-mouth disinfection and systemic antimicrobial therapy in generalized aggressive periodontitis: a randomized, placebo-controlled trial. | aim: the present investigation aimed to analyse clinical and microbiological effects of systemic administration of metronidazole and amoxicillin combined with the one-stage-full-mouth-disinfection protocol (osfmd) in generalized aggressive periodontitis patients (g-agp). materials and methods: thirty-nine systemically healthy patients with g-agp were consecutively included. the test group (n = 19) received amoxicillin-metronidazole combination (500 mg of each, three times a day for 7 days) and t ... | 2012 | 22220822 |
| sialic acid, periodontal pathogens and tannerella forsythia: stick around and enjoy the feast! | periodontal pathogens, like any other human commensal or pathogenic bacterium, must possess both the ability to acquire the necessary growth factors and the means to adhere to surfaces or reside and survive in their environmental niche. recent evidence has suggested that sialic acid containing host molecules may provide both of these requirements in vivo for several periodontal pathogens but most notably for the red complex organism tannerella forsythia. several other periodontal pathogens also ... | 2012 | 22230462 |
| sialic acid, periodontal pathogens and tannerella forsythia: stick around and enjoy the feast! | periodontal pathogens, like any other human commensal or pathogenic bacterium, must possess both the ability to acquire the necessary growth factors and the means to adhere to surfaces or reside and survive in their environmental niche. recent evidence has suggested that sialic acid containing host molecules may provide both of these requirements in vivo for several periodontal pathogens but most notably for the red complex organism tannerella forsythia. several other periodontal pathogens also ... | 2012 | 22230462 |
| periodontal pathogens affect the level of protease inhibitors in gingival crevicular fluid. | in periodontitis, an effective host-response is primarily related to neutrophils loaded with serine proteases, including elastase (ne) and protease 3 (pr3), the extracellular activity of which is tightly controlled by endogenous inhibitors. in vitro these inhibitors are degraded by gingipains, cysteine proteases produced by porphyromonas gingivalis. the purpose of this study was to determine the level of selected protease inhibitors in gingival crevicular fluid (gcf) in relation to periodontal i ... | 2012 | 22230465 |
| polymicrobial interactions: impact on pathogenesis and human disease. | summary: microorganisms coexist in a complex milieu of bacteria, fungi, archaea, and viruses on or within the human body, often as multifaceted polymicrobial biofilm communities at mucosal sites and on abiotic surfaces. only recently have we begun to appreciate the complicated biofilm phenotype during infection; moreover, even less is known about the interactions that occur between microorganisms during polymicrobial growth and their implications in human disease. therefore, this review focuses ... | 2012 | 22232376 |
| porphyromonas endodontalis in chronic periodontitis: a clinical and microbiological cross-sectional study. | although previous studies have shown the presence of porphyromonas endodontalis in chronic periodontitis associated with periapical lesions, the occurrence of this pathogen in diseased periodontal sites without periapical lesions has been poorly investigated. | 2012 | 22232719 |
| impact of supragingival therapy on subgingival microbial profile in smokers versus non-smokers with severe chronic periodontitis. | the aim of this study was to assess subgingival microbiological changes in smokers versus non-smokers presenting severe chronic periodontitis after supragingival periodontal therapy (st). | 2012 | 22232720 |
| development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete treponema denticola. | herein, we report that a modified gentamicin cassette and a pcr-based method can be used for targeted mutagenesis of the oral spirochete treponema denticola. this approach minimizes polar effects and spontaneous antibiotic resistance. therefore, it can serve as a reliable tool for genetic manipulation of t. denticola. | 2012 | 22247130 |
| filament formation associated with spirochetal infection: a comparative approach to morgellons disease. | bovine digital dermatitis is an emerging infectious disease that causes lameness, decreased milk production, and weight loss in livestock. proliferative stages of bovine digital dermatitis demonstrate keratin filament formation in skin above the hooves in affected animals. the multifactorial etiology of digital dermatitis is not well understood, but spirochetes and other coinfecting microorganisms have been implicated in the pathogenesis of this veterinary illness. morgellons disease is an emerg ... | 2011 | 22253541 |
| inflammasomes in cardiovascular diseases. | nod-like receptors (nlrs) constitute a recently identified family of macromolecules that participate in regulation of innate immune responses. to date, 23 members of the nlr family are identified in humans. diverse nlrs are stimulated by a broad range of pathogen- or danger-associated molecular patterns, and collectively function as intracellular pattern recognition receptors (prrs). the most studied inflammasomes are nlrp1 and nlrp3 that process inactive pro-caspase-1 to its active form, allowi ... | 2011 | 22254202 |
| microbial profile of patients with periodontitis compared with healthy subjects. | objective: to define and compare the microbiologic profile of subgingival plaque in german patients with periodontitis (including aggressive and advanced chronic periodontitis) and healthy subjects and to determine significant association between isolates and clinical status. aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia are major periodontal pathogens, though it is recognized that other species may also contribute to the pathogenesis of periodontal di ... | 2012 | 22257880 |
| necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study. | objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ... | 2012 | 22259811 |
| on the acquisition of periodontopathic bacteria by children from mothers: a randomized double-masked placebo-controlled trial in bauru, brazil. | objective: the purpose of this study was two-fold: 1) to determine the effects of mechanical and chemical modalities treatments on periodontal clinical and microbiological parameters of mothers; and 2) to determine the subsequent colonization of periodontal pathogens in tongue samples from their infants. design: a total of 168 mothers met inclusion criteria to participate in a randomized double-masked placebo-controlled clinical trial. of those, 121 mothers (and their infants) of 158 seen at bas ... | 2006 | 22267892 |
| analysis of the cell surface layer ultrastructure of the oral pathogen tannerella forsythia. | the gram-negative oral pathogen tannerella forsythia is decorated with a 2d crystalline surface (s-) layer, with two different s-layer glycoprotein species being present. prompted by the predicted virulence potential of the s-layer, this study focused on the analysis of the arrangement of the individual s-layer glycoproteins by a combination of microscopic, genetic, and biochemical analyses. the two s-layer genes are transcribed into mrna and expressed into protein in equal amounts. the s-layer ... | 2012 | 22273979 |
| beta-hexosaminidase activity of the oral pathogen tannerella forsythia influences biofilm formation on glycoprotein substrates. | tannerella forsythia is an important pathogen in periodontal disease. previously, we showed that its sialidase activity is key to utilization of sialic acid from a range of human glycoproteins for biofilm growth and initial adhesion. removal of terminal sialic acid residues often exposes β-linked glucosamine or galactosamine, which may also be important adhesive molecules. in turn, these residues are often removed by a group of enzymes known as β-hexosaminidases. we show here that t. forsythia h ... | 2012 | 22276920 |
| a metalloproteinase karilysin present in the majority of tannerella forsythia isolates inhibits all pathways of the complement system. | tannerella forsythia is a poorly studied pathogen despite being one of the main causes of periodontitis, which is an inflammatory disease of the supporting structures of the teeth. we found that despite being recognized by all complement pathways, t. forsythia is resistant to killing by human complement, which is present at up to 70% of serum concentration in gingival crevicular fluid. incubation of human serum with karilysin, a metalloproteinase of t. forsythia, resulted in a decrease in bacter ... | 2012 | 22287711 |
| analysis of the salivary microbiome using culture-independent techniques. | the salivary microbiota is a potential diagnostic indicator of several diseases. culture-independent techniques are required to study the salivary microbial community since many of its members have not been cultivated. | 2012 | 22300522 |
| detection of periodontal bacteria in thrombi of patients with acute myocardial infarction by polymerase chain reaction. | numerous reports have demonstrated that periodontal bacteria are present in plaques from atherosclerotic arteries. although periodontitis has recently been recognized as a risk factor for coronary artery disease, the direct relationship between periodontal bacteria and coronary artery disease has not yet been clarified. it has been suggested that these bacteria might contribute to inflammation and plaque instability. we assumed that if periodontal bacteria induce inflammation of plaque, the bact ... | 2012 | 22305832 |
| structural, bioinformatic, and in vivo analyses of two treponema pallidum lipoproteins reveal a unique trap transporter. | treponema pallidum, the bacterial agent of syphilis, is predicted to encode one tripartite atp-independent periplasmic transporter (trap-t). trap-ts typically employ a periplasmic substrate-binding protein (sbp) to deliver the cognate ligand to the transmembrane symporter. herein, we demonstrate that the genes encoding the putative trap-t components from t. pallidum, tp0957 (the sbp), and tp0958 (the symporter), are in an operon with an uncharacterized third gene, tp0956. we determined the cryst ... | 2012 | 22306465 |
| treponema denticola chymotrypsin-like proteinase (ctlp) integrates spirochaetes within oral microbial communities. | treponema denticola is found ubiquitously in the human oral cavity and is mainly associated with bacterial communities implicated in the establishment and development of periodontal disease. the ability to become integrated within biofilm communities is crucial to the growth and survival of oral bacteria, and involves inter-bacterial coaggregation, metabolic cooperation, and synergy against host defences. in this article we show that the chymotrypsin-like proteinase (ctlp), found within a high-m ... | 2012 | 22313692 |
| molecular analysis of 16s rrna genes identifies potentially periodontal pathogenic bacteria and archaea in the plaque of partially erupted third molars. | small subunit rrna sequencing and phylogenetic analysis were used to identify cultivable and uncultivable microorganisms present in the dental plaque of symptomatic and asymptomatic partially erupted third molars to determine the prevalence of putative periodontal pathogens in pericoronal sites. | 2012 | 22326171 |
| effect of periodontal pathogens on the metatranscriptome of a healthy multispecies biofilm model. | oral bacterial biofilms are highly complex microbial communities with up to 700 different bacterial taxa. we report here the use of metatranscriptomic analysis to study patterns of community gene expression in a multispecies biofilm model composed of species found in healthy oral biofilms (actinomyces naeslundii, lactobacillus casei, streptococcus mitis, veillonella parvula, and fusobacterium nucleatum) and the same biofilm plus the periodontopathogens porphyromonas gingivalis and aggregatibacte ... | 2012 | 22328675 |
| microbiologic profile of endodontic infections from hiv- and hiv+ patients using multiple-displacement amplification and checkerboard dna-dna hybridization. | to compare the microbiota of endodontic infections in necrotic pulp from hiv-negative and hiv-positive subjects. | 2012 | 22335194 |
| the relationship between turbidity of mouth-rinsed water and oral health status. | the purpose of this study was to examine the relationship between turbidity of mouth rinsed water and oral health status such as dental and periodontal conditions, oral hygiene status, flow rate of saliva and oral bacteria. | 2013 | 22339260 |
| involvement of a periodontal pathogen, porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease. | non-alcoholic fatty liver disease (nafld) is a hepatic manifestation of metabolic syndrome that is closely associated with multiple factors such as obesity, hyperlipidemia and type 2 diabetes mellitus. however, other risk factors for the development of nafld are unclear. with the association between periodontal disease and the development of systemic diseases receiving increasing attention recently, we conducted this study to investigate the relationship between nafld and infection with porphyro ... | 2012 | 22340817 |
| rheumatoid arthritis and periodontitis - inflammatory and infectious connections. review of the literature. | an association between oral disease/periodontitis and rheumatoid arthritis (ra) has been considered since the early 1820s. the early treatment was tooth eradication. epidemiological studies suggest that the prevalence of ra and periodontitis may be similar and about 5% of the population are aged 50 years or older. ra is considered as an autoimmune disease whereas periodontitis has an infectious etiology with a complex inflammatory response. both diseases are chronic and may present with bursts o ... | 2012 | 22347541 |
| [relationship between the preterm low birth weight infant and the periodontal pathogen bacteria in maternal saliva]. | to investigate the possible relationship between the preterm and/or low birth weight (plbw) and three anaerobic microorganisms in saliva of their mothers, including porphyromonas gingivalis (pg), tannerella forsythia (tf), treponema denticola (td). | 2012 | 22353895 |
| bacterial biogeography of the human digestive tract. | we present bacterial biogeography as sampled from the human gastrointestinal tract of four healthy subjects. this study generated >32 million paired-end sequences of bacterial 16s rrna genes (v3 region) representing >95,000 unique operational taxonomic units (otus; 97% similarity clusters), with >99% good's coverage for all samples. the highest otu richness and phylogenetic diversity was found in the mouth samples. the microbial communities of multiple biopsy sites within the colon were highly s ... | 2011 | 22355685 |
| saliva: a diagnostic biomarker of periodontal diseases. | early detection of disease plays a crucial role in successful therapy. early diagnosis and management reduces the severity and possible complications of the disease process. to overcome this challenge, medical researchers are devoted to finding molecular disease biomarkers that reveal a hidden lethal threat before the disease becomes complicated. saliva, an important physiologic fluid, containing a highly complex mixture of substances, is rapidly gaining popularity as a diagnostic tool. periodon ... | 2011 | 22368352 |
| treponema denticola interactions with host proteins. | oral treponema species, most notably t. denticola, are implicated in the destructive effects of human periodontal disease. progress in the molecular analysis of interactions between t. denticola and host proteins is reviewed here, with particular emphasis on the characterization of surface-expressed and secreted proteins of t. denticola involved in interactions with host cells, extracellular matrix components, and components of the innate immune system. | 2012 | 22368767 |
| host-pathogen dynamics: it's complicated! | 2012 | 22368768 | |
| oral microbiota in swiss adolescents. | the purpose of the study was to determine the prevalence of different oral microbes in gingival plaque samples and in samples from the dorsum of the tongue in a swiss adolescent population. | 2013 | 22371122 |
| structure of factor h-binding protein b (fhbb) of the periopathogen, treponema denticola: insights into progression of periodontal disease. | periodontitis is the most common disease of microbial etiology in humans. periopathogen survival is dependent upon evasion of complement-mediated destruction. treponema denticola, an important contributor to periodontitis, evades killing by the alternative complement cascade by binding factor h (fh) to its surface. bound fh is rapidly cleaved by the t. denticola protease, dentilisin. in this report, the structure of the t. denticola fh-binding protein, fhbb, was solved to 1.7 å resolution. fhbb ... | 2012 | 22371503 |
| anaerobic and microaerophilic pathogens in the biofilm formation on voice prostheses: a pilot study. | voice rehabilitation with voice prostheses is a standard therapy in laryngectomized patients. biofilm formation on the surface of the voice prostheses causes device failure and requires frequent replacements. studies analyzing the biofilm of voice prostheses have mainly focused on aerobic bacteria. anaerobic bacteria as an integral part of the biofilms on voice prostheses have not been investigated yet. | 2012 | 22374743 |
| tprc/d (tp0117/131), a trimeric, pore-forming rare outer membrane protein of treponema pallidum, has a bipartite domain structure. | identification of treponema pallidum rare outer membrane proteins (omps) has been a longstanding objective of syphilis researchers. we recently developed a consensus computational framework that employs a battery of cellular localization and topological prediction tools to generate ranked clusters of candidate rare omps (d. l. cox et al., infect. immun. 78:5178-5194, 2010). tp0117/tp0131 (tprc/d), a member of the t. pallidum repeat (tpr) family, was a highly ranked candidate. circular dichroism, ... | 2012 | 22389487 |
| understanding the roles of gingival beta-defensins. | gingival epithelium produces β-defensins, small cationic peptides, as part of its contribution to the innate host defense against the bacterial challenge that is constantly present in the oral cavity. besides their functions in healthy gingival tissues, β-defensins are involved in the initiation and progression, as well as restriction of periodontal tissue destruction, by acting as antimicrobial, chemotactic, and anti-inflammatory agents. in this article, we review the common knowledge about β-d ... | 2012 | 22389759 |
| comparative evaluation of the effects of different photoablative laser irradiation protocols on the gingiva of periodontopathic patients. | we aimed at quantifying the presence of periodontopathogens in gingival biopsies from periodontitis patients treated with different photoablative lasers (diode gaas, er:yag, nd:yag, and co(2) lasers) and histologically analyzing their effects on the gingiva. | 2012 | 22401128 |
| distribution of periodontopathic bacterial species in dogs and their owners. | presently, a large number of individuals consider their companion animals as family members and have close contact with them in daily life. the purpose of the present study was to analyze the distribution of periodontopathic bacterial species in oral specimens taken from dogs and their owners. | 2012 | 22417880 |
| microbial profiles of patients seeking treatment for periodontitis. influence of origin, smoking and age? | we assessed the potential influence of the origin, the smoking status and the age on subgingival microbial profiles of subjects seeking periodontal care in switzerland today. | 2012 | 22419513 |
| non-inflammatory destructive periodontal disease: a clinical, microbiological, immunological and genetic investigation. | periodontitis comprises a group of multifactorial diseases in which periodontopathogens accumulate in dental plaque and trigger host chronic inflammatory and immune responses against periodontal structures, which are determinant to the disease outcome. although unusual cases of non-inflammatory destructive periodontal disease (nidpd) are described, their pathogenesis remains unknown. a unique nidpd case was investigated by clinical, microbiological, immunological and genetic tools. the patient, ... | 2012 | 22437688 |
| adjunctive azithromycin in the treatment of aggressive periodontitis: microbiological findings of a 12-month randomized clinical trial. | to compare the subgingival microbiological outcomes of azithromycin or placebo as adjuncts to scaling and root planing (srp) in the treatment of aggressive periodontitis (agp), and to secondarily evaluate the microbiological effect of supragingival scaling in agp patients. | 2012 | 22445846 |
| features of two proteins of leptospira interrogans with potential role in host-pathogen interactions. | leptospirosis is considered a re-emerging infectious disease caused by pathogenic spirochaetes of the genus leptospira. pathogenic leptospires have the ability to survive and disseminate to multiple organs after penetrating the host. leptospires were shown to express surface proteins that interact with the extracellular matrix (ecm) and to plasminogen (plg). this study examined the interaction of two putative leptospiral proteins with laminin, collagen type i, collagen type iv, cellular fibronec ... | 2012 | 22463075 |
| biofilm: a dental microbial infection. | recent advances in research technology have allowed researchers to study bacteria in their natural environment. dental biofilm forms via an ordered sequence of events, resulting in structured and functionally organized species rich microbial community and modern molecular biological techniques have identified about 1000 different bacterial species in the dental biofilm, twice as many as can be cultured. sites for biofilm formation include all kinds of surfaces: natural materials above and below ... | 2011 | 22470238 |
| intraperiodontal pocket: an ideal route for local antimicrobial drug delivery. | periodontal pockets act as a natural reservoir filled with gingival crevicular fluid for the controlled release delivery of antimicrobials directly. this article reflects the present status of nonsurgical controlled local intrapocket delivery of antimicrobials in the treatment of periodontitis. these sites have specialty in terms of anatomy, permeability, and their ability to retain a delivery system for a desired length of time. a number of antimicrobial products and the composition of the deli ... | 2012 | 22470888 |
| borrelia burgdorferi needs chemotaxis to establish infection in mammals and to accomplish its enzootic cycle. | borrelia burgdorferi, the causative agent of lyme disease, can be recovered from different organs of infected animals and patients, indicating that the spirochete is very invasive. motility and chemotaxis contribute to the invasiveness of b. burgdorferi and play important roles in the process of the disease. recent reports have shown that motility is required for establishing infection in mammals. however, the role of chemotaxis in virulence remains elusive. our previous studies showed that chea ... | 2012 | 22508862 |
| bactquant: an enhanced broad-coverage bacterial quantitative real-time pcr assay. | bacterial load quantification is a critical component of bacterial community analysis, but a culture-independent method capable of detecting and quantifying diverse bacteria is needed. based on our analysis of a diverse collection of 16 s rrna gene sequences, we designed a broad-coverage quantitative real-time pcr (qpcr) assay--bactquant--for quantifying 16 s rrna gene copy number and estimating bacterial load. we further utilized in silico evaluation to complement laboratory-based qpcr characte ... | 2012 | 22510143 |
| probiotics and periodontal health. | periodontitis is one of the most common chronic inflammatory diseases. the etiology is clearly bacterial and a number of putative bacterial pathogens have been associated with the disease, including aggregatibacter actinomycetemcomitans, tannerella forsythus and porphyromonas gingivalis. comparatively, little attention has been paid to the identification of health-associated and potentially beneficial bacterial species that may reside in the gingival sulcus. probiotic technology represents a bre ... | 2011 | 22514571 |
| acquisition of complement inhibitor serine protease factor i and its cofactors c4b-binding protein and factor h by prevotella intermedia. | infection with the gram-negative pathogen prevotella intermedia gives rise to periodontitis and a growing number of studies implies an association of p. intermedia with rheumatoid arthritis. the serine protease factor i (fi) is the central inhibitor of complement degrading complement components c3b and c4b in the presence of cofactors such as c4b-binding protein (c4bp) and factor h (fh). yet, the significance of complement inhibitor acquisition in p. intermedia infection and fi binding by gram-n ... | 2012 | 22514678 |
| regulation of mnth by a dual mn(ii)- and fe(ii)-dependent transcriptional repressor (dr2539) in deinococcus radiodurans. | the high intracellular mn/fe ratio observed within the bacteria deinococcus radiodurans may contribute to its remarkable resistance to environmental stresses. we isolated dr2539, a novel regulator of intracellular mn/fe homeostasis in d. radiodurans. electrophoretic gel mobility shift assays (emsas) revealed that dr2539 binds specifically to the promoter of the manganese acquisition transporter (mnth) gene, and that dr0865, the only fur homologue in d. radiodurans, cannot bind to the promoter of ... | 2012 | 22523570 |
| quantification of subgingival bacterial pathogens at different stages of periodontal diseases. | anaerobic gram-negative oral bacteria such as treponema denticola, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsythia, campylobacter rectus, and fusobacterium nucleatum are closely associated with periodontal diseases. we measured the relative population (bacterial levels) of these oral pathogens in subgingival tissues of patients at different stages of korean chronic periodontal diseases. we divided the individuals into those with chronic gingivitis (g), moder ... | 2012 | 22526568 |
| chemically modified tetracyclines: novel therapeutic agents in the management of chronic periodontitis. | chronic periodontitis is a complex infection initiated by gram-negative bacteria which destroy the supporting structures of the tooth. recently, it has been recognized that it is the host response to bacterial infection which causes greater destruction of the connective tissue elements, periodontal ligament and alveolar bone in periodontitis. this has led to the development of various host modulating approaches to target cells and their destructive mediators involved in tissue degradation. chemi ... | 2012 | 22529468 |
| oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients. | oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (hsct). through hsct, the homeostasis in the oral cavity is disrupted. the contribution of the oral microflora to mucositis remains to be clarified. the aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in hsct recipients. | 2012 | 22531876 |
| disabled patients and oral health in rome, italy: long-term evaluation of educational initiatives. | this study is concerned with the educational intervention layout proposed as a possible answer for the disparities in healthcare services for disabled persons. | 2012 | 22545186 |
| disabled patients and oral health in rome, italy: long-term evaluation of educational initiatives. | this study is concerned with the educational intervention layout proposed as a possible answer for the disparities in healthcare services for disabled persons. | 2012 | 22545186 |
| serum antibodies to periodontal pathogens are a risk factor for alzheimer's disease. | chronic inflammation in periodontal disease has been suggested as a potential risk factor in alzheimer's disease (ad). the purpose of this study was to examine serum antibody levels to bacteria of periodontal disease in participants who eventually converted to ad compared with the antibody levels in control subjects. | 2012 | 22546352 |
| fusobacterium nucleatum and tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model. | tannerella forsythia is strongly associated with chronic periodontitis, an inflammatory disease of the tooth-supporting tissues, leading to tooth loss. fusobacterium nucleatum, an opportunistic pathogen, is thought to promote dental plaque formation by serving as a bridge bacterium between early- and late-colonizing species of the oral cavity. previous studies have shown that f. nucleatum species synergize with t. forsythia during biofilm formation and pathogenesis. in the present study, we show ... | 2012 | 22547549 |
| prevalence of fima genotypes of porphyromonas gingivalis and other periodontal bacteria in a spanish population with chronic periodontitis. | the aim of this study was to determine the prevalence of the different fima genotypes of porphyromonas gingivalis in adult spanish patients with chronic periodontitis, patients with gingivitis and periodontally healthy subjects, and the relationship between these genotypes and other periodontopathogenic bacteria. | 2012 | 22549664 |
| identification of treponema denticola in subgingival samples by pcr technology and its correlation with clinical diagnosis. | treponema denticola has been associated with gingivitis and chronic periodontitis. the aim of this study was to identify treponema denticola in subgingival samples using pcr technology and to correlate it with clinical diagnosis of subjects. the study was carried out on seventy patients (20-84 years of age; mean age, 45.06 +/- 12.58) of which 22 individuals with no detectable gingivitis or periodontitis, 4 subjects with chronic gingivitis and 44 subjects with chronic periodontitis. subgingival p ... | 2012 | 22568260 |
| the short-term treatment effects on the microbiota at the dorsum of the tongue in intra-oral halitosis patients--a randomized clinical trial. | this study aims to assess the effects of rinsing with zinc- and chlorhexidine-containing mouth rinse with or without adjunct tongue scraping on volatile sulfur compounds (vscs) in breath air, and the microbiota at the dorsum of the tongue. | 2013 | 22573244 |
| relationship between oral anaerobic bacteria and otitis media with effusion. | in this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (ome), we aimed to investigate the presence and similarity of fusobacterium nucleatum and treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with ome. | 2012 | 22606045 |
| integrin α5β1 activates the nlrp3 inflammasome by direct interaction with a bacterial surface protein. | integrins are cell-surface heterodimeric glycoproteins composed of alpha and beta subunits that mediate cell-cell, cell-extracellular matrix, and cell-pathogen interactions. in this study, we report a specific role of integrin α5β1 in nlrp3 inflammasome activation in macrophages stimulated by td92, a surface protein of the periodontopathogen, treponema denticola. the direct interaction of td92 with the cell membrane integrin α5β1 resulted in atp release and k(+) efflux, which are the main events ... | 2012 | 22608495 |
| spontaneous periodontitis development in diabetic rats involves an unrestricted expression of inflammatory cytokines and tissue destructive factors in the absence of major changes in commensal oral microbiota. | diabetes mellitus is a heterogeneous group of disorders, in which hyperglycemia is a main feature. the objective was to evaluate the involvement of rage, inflammatory cytokines, and metalloproteinases in spontaneous periodontitis triggered by diabetes induction. immunohistochemical procedures for mmp-2, mmp-9, tnf-α, il-1β, il-6, rankl, and rage were performed in rats after 1, 3, 6, 9, and 12 months of diabetes induction. total dna was extracted from paraffin-embedded tissues and evaluated by re ... | 2012 | 22611374 |
| identification of an intracellular m17 family leucine aminopeptidase that is required for virulence in staphylococcus aureus. | staphylococcus aureus is a highly virulent bacterial pathogen capable of causing a variety of ailments throughout the human body. it is a major public health concern due to the continued emergence of highly pathogenic methicillin resistant strains (mrsa) both within hospitals and in the community. virulence in s. aureus is mediated by an array of secreted and cell wall associated virulence factors, including toxins, hemolysins and proteases. in this work we identify a leucine aminopeptidase (lap ... | 2012 | 22613209 |
| the rickettsial ompb β-peptide of rickettsia conorii is sufficient to facilitate factor h-mediated serum resistance. | pathogenic species of the spotted fever group rickettsia are subjected to repeated exposures to the host complement system through cyclic infections of mammalian and tick hosts. the serum complement machinery is a formidable obstacle for bacteria to overcome if they endeavor to endure this endozoonotic cycle. we have previously demonstrated that that the etiologic agent of mediterranean spotted fever, rickettsia conorii, is susceptible to complement-mediated killing only in the presence of speci ... | 2012 | 22615250 |
| causes of adverse pregnancy outcomes and the role of maternal periodontal status - a review of the literature. | preterm (pt) and low birth weight (lbw) are considered to be the most relevant biological determinants of newborn infants survival, both in developed and in developing countries. numerous risk factors for pt and lbw have been defined in the literature. infections of the genitourinary tract infections along with various biological and genetic factors are considered to be the most common etiological factors for pt/lbw deliveries. however, evidence suggests that sub-clinical infection sites that ar ... | 2012 | 22615718 |