Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| interactions of phocein with nucleoside-diphosphate kinase, eps15, and dynamin i. | phocein, an intracellular protein interacting with striatin, bears a few homologies with the sigma-subunits of clathrin adaptor proteins (baillat, g., moqrich, a., castets, f., baude, a., bailly, y., benmerah, a., and monneron, a. (2001) mol. biol. cell 12, 663-673). using phocein as a bait in a yeast two-hybrid screen, we identified two novel interacting proteins, nucleoside-diphosphate kinase (ndpk) and eps15. immunoprecipitation and pull-down experiments involving native and/or recombinant ph ... | 2002 | 11872741 |
| uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived l6 cells. | the uncoupling proteins (ucps) are thought to uncouple oxidative phosphorylation in the mitochondria and thus generate heat. one of the ucp isoforms, ucp3, is abundantly expressed in skeletal muscle, the major thermogenic tissue in humans. ucp3 has been overexpressed at high levels in yeast systems, where it leads to the uncoupling of cell respiration, suggesting that ucp3 may indeed be capable of dissipating the mitochondrial proton gradient. this effect, however, was recently shown to be a con ... | 2002 | 11874451 |
| cxcr4/ccr5 down-modulation and chemotaxis are regulated by the proteasome pathway. | chemokines and their receptors play a critical role in host immune surveillance and are important mediators of human immunodeficiency virus (hiv) pathogenesis and inflammatory response. the chemokine receptors ccr5 and cxcr4, which act as co-receptors along with cd4 for hiv docking and entry, are down-modulated by their respective ligands, mip-1beta/sdf-1alpha or by the hiv envelope protein, gp120. we have studied the role of the proteasome pathway in the down-regulation of these receptors. usin ... | 2002 | 11877445 |
| cd40 induces interleukin-6 gene transcription in dendritic cells: regulation by traf2, ap-1, nf-kappa b, and cbf1. | cd40-induced activation of cytokine gene expression in dendritic cells (dc) is an important process in the initiation of primary immune responses. we have determined the intracellular signaling events that lead to cd40 ligation-induced activation of interleukin-6 (il-6) gene transcription in a murine dc line, fsdc, that is phenotypically representative of bone marrow-derived dc. il-6 reverse transcriptase-pcr and promoter assays established the responsiveness of fsdc to anti-cd40 ligation. furth ... | 2002 | 11886848 |
| murine apg12p has a substrate preference for murine apg7p over three apg8p homologs. | apg7p is a unique e1 enzyme which is essential for both the apg12p- and apg8p-modification systems, and plays indispensable roles in yeast autophagy. a cdna encoding murine apg7p homolog (mapg7p) was isolated from a mouse brain cdna library. the predicted amino acid sequence of the clone shows a significant homology to human apg7p and yeast apg7p. murine apg12p as well as the three mammalian apg8p homologs co-immunoprecipitate with mapg7p. site-directed mutagenesis revealed that an active-site c ... | 2002 | 11890701 |
| bioavailability of selenium from raw or cured selenomethionine-enriched fillets of atlantic salmon (salmo salar) assessed in selenium-deficient rats. | the bioavailability of se from raw and cured selenomethionine-enriched (se-enriched) salmon fillets was assessed in se-deficient male albino rats (mol: wist). a low-se torula yeast feed was supplemented with 0, 50, 100, 150 or 200 microg se/kg as sodium selenite or as se from raw or cured se-enriched salmon. the diets were fed to weanling rats for 10 and 30 d. bioavailability of se was assessed by metabolic balance, se accumulation in femur, muscle, liver and plasma, and induction of se-dependen ... | 2002 | 11895311 |
| cdc6 requires anchorage for its expression. | fibroblasts need anchorage to extracellular matrix to transit from g1 to s phase, but no longer after oncogenic transformation. here we report that cdc6 protein essential for the activation of replication origins requires anchorage or oncogenic stimulation for its execution. upon anchorage loss, cdc6 expression is shut off both transcriptionally and post-transcriptionally in a rat fibroblast despite enforced activation of e2f-dependent promoters. however, stimulation of this cell with oncogenic ... | 2002 | 11896609 |
| expression and localization of the multidrug resistance-associated protein 3 in rat small and large intestine. | multidrug resistance-associated protein 3 (mrp3; symbol abcc3), has been shown to mediate atp-dependent transport of organic anions including 17beta-glucuronosyl estradiol, glucuronosyl bilirubin, monovalent, and sulfated bile salts. mrp3 mrna expression was reported in rat intestine suggesting a role of mrp3 in the intestinal transport. we examined the expression and localization of mrp3 in rat small and large intestine by rt-pcr, immunofluorescence, and immunoblot analysis. mrp3 was identified ... | 2002 | 11897632 |
| role of nuclear factor-kappa b in the activation of alveolar macrophages by fungal beta-glucans. | 2001 | 11906043 | |
| role of adaptor complex ap-3 in targeting wild-type and mutated cd63 to lysosomes. | cd63 is a lysosomal membrane protein that belongs to the tetraspanin family. its carboxyterminal cytoplasmic tail sequence contains the lysosomal targeting motif gyevm. strong, tyrosine-dependent interaction of the wild-type carboxyterminal tail of cd63 with the ap-3 adaptor subunit mu 3 was observed using a yeast two-hybrid system. the strength of interaction of mutated tail sequences with mu 3 correlated with the degree of lysosomal localization of similarly mutated human cd63 molecules in sta ... | 2002 | 11907283 |
| mutations in conserved regions 1, 2, and 3 of raf-1 that activate transforming activity. | to investigate the role of raf-1 in v-ha-ras transformation, we have isolated and characterized a number of raf-1 mutants that display increased transforming activity in rat2 fibroblasts. a dipeptide deletion (delta144-145) in the cysteine-rich domain (crd) of conserved region (cr) 1 increased the interaction between raf-1 and v-ha-ras effector loop mutants in the yeast two-hybrid system, supporting the proposal that the crd serves as a secondary ras-binding domain. many activating mutations wer ... | 2002 | 11933072 |
| subunit specificity and interaction domain between gaba(a) receptor-associated protein (gabarap) and gaba(a) receptors. | gabarap (gaba(a) receptor-associated protein) interacts with both microtubules and gaba(a) receptors in vitro and in vivo and is capable of modulating receptor channel kinetics. in this study, we use the intracellular loop of 15 gaba(a) receptor subunits to show that the interaction between gabarap and gaba(a) receptor is specific for the gamma subunits. pharmacological characterization of proteins purified by gabarap affinity column indicates that native gaba(a) receptors interact with gabarap. ... | 2002 | 11948245 |
| selective association of protein kinase c with 14-3-3 zeta in neuronally differentiated pc12 cells. stimulatory and inhibitory effect of 14-3-3 zeta in vivo. | the 14-3-3 protein is a family of highly conserved acidic proteins found in a wide range of eukaryotes from yeast to mammals. 14-3-3 acts as an adapter protein and interacts with signaling molecules including protein kinase c (pkc). although 14-3-3 zeta was originally characterized as an endogenous pkc inhibitor, it was reported to activate pkc in vitro, but the in vivo regulation of pkc by 14-3-3 is still not well understood. to examine the regulation of pkc by 14-3-3 in the cell, we have gener ... | 2002 | 11950841 |
| analysis of nsf mutants reveals residues involved in snap binding and atpase stimulation. | n-ethylmaleimide-sensitive fusion protein (nsf) and its yeast orthologue, sec18, are cytoplasmic aaa(+) atpases required for most intracellular membrane fusion events. the primary function of nsf is thought to be the disassembly of cis-snare complexes, thus allowing trans-snare complex formation and subsequent membrane fusion. the importance of nsf/sec18 in intracellular membrane traffic in vivo is highlighted by the inhibition of neurotransmission in drosophila comatose (nsf) mutants and of con ... | 2002 | 11955072 |
| distinct snare complexes mediating membrane fusion in golgi transport based on combinatorial specificity. | syntaxin-5 (sed5) is the only syntaxin needed for transport into and across the yeast golgi, raising the question of how a single syntaxin species could mediate vesicle transport in both the anterograde and the retrograde direction within the stack. sed5 is known to combine with two light chains (bos1 and sec22) to form the t-snare needed to receive vesicles from the endoplasmic reticulum. however, the yeast golgi contains several other potential light chains with which sed5 could potentially co ... | 2002 | 11959998 |
| glutathione synthetase from plasmodium falciparum. | gsh is the major low-molecular-mass thiol in most organisms. the tripeptide maintains a reduced intracellular environment and protects cellular components from damaging oxidation. gsh is synthesized by the action of two atp-dependent enzymic steps, in which gamma-glutamylcysteine synthetase (gamma-gcs) catalyses the ligation of glutamate and cysteine and subsequently glutathione synthetase (gs) adds glycine to the dipeptide. recently it was shown that the synthesis of gamma-glutamylcysteine is c ... | 2002 | 11964186 |
| binding to cisplatin-modified dna by the saccharomyces cerevisiae hmgb protein nhp6a. | nhp6a is an abundant non-histone chromatin-associated protein in saccharomyces cerevisiae that contains a minor groove dna binding motif called the hmg box. in this report, we show that nhp6ap binds to cisplatin intrastrand cross-links on duplex dna with a 40-fold greater affinity than to unmodified dna with the same sequence. nevertheless, nhp6ap bound to cisplatinated dna readily exchanges onto unmodified dna. phenanthroline-copper footprinting and two-dimensional nmr on complexes of wild-type ... | 2002 | 11969400 |
| tyrosine phosphorylation regulates alpha ii spectrin cleavage by calpain. | spectrins, components of the membrane skeleton, are implicated in various cellular functions. understanding the diversity of these functions requires better characterization of the interacting domains of spectrins, such as the sh3 domain. yeast two-hybrid screening of a kidney cdna library revealed that the sh3 domain of alpha ii-spectrin binds specifically isoform a of low-molecular-weight phosphotyrosine phosphatase (lmw-ptp). the alpha ii-spectrin sh3 domain does not interact with lmw-ptp b o ... | 2002 | 11971983 |
| emi1 is required for cytostatic factor arrest in vertebrate eggs. | vertebrate eggs are arrested at metaphase of meiosis ii with stable cyclin b and high cyclin b/cdc2 kinase activity. the ability of the anaphase-promoting complex/cyclosome (apc), an e3 ubiquitin ligase, to trigger cyclin b destruction and metaphase exit is blocked in eggs by the activity of cytostatic factor (csf) (reviewed in ref. 1). csf was defined as an activity in mature oocytes that caused mitotic arrest when injected into dividing embryos. fertilization causes a transient increase in cyt ... | 2002 | 11976684 |
| probing electrostatic interactions in cytochrome c using site-directed chemical modification. | this communication reports the generation of an electrostatic probe using chemical modification of methionine side chains. the alkylation of methionine by iodoacetamide was achieved in a set of saccharomyces cerevisiae iso-1-cytochrome c mutants, introducing the nontitratable, nondelocalized positive charge of a carboxyamidomethylmethionine sulfonium (camms) ion at five surface and one buried site in the protein. changes in redox potential and its variation with temperature were used to calculat ... | 2002 | 11989715 |
| ligand-selective potentiation of rat mineralocorticoid receptor activation function 1 by a cbp-containing histone acetyltransferase complex. | the rat mineralocorticoid receptor (mr) has two activation functions in distinct regions of the a/b domain, designated activation function 1a (af-1a; amino acids 1 to 169) and af-1b (amino acids 451 to 600). since the p160 family protein tif2, a known component of the af-2 coactivator complex, potentiates the transactivation function of af-1b but not that of af-1a, it is likely that some other, novel protein complex interacts with the af-1a region. therefore, we attempted to identify such coacti ... | 2002 | 11997506 |
| confirmation of uterotrophic activity of 3-(4-methylbenzylidine)camphor in the immature rat. | in this study we found that the ultraviolet sunscreen component 3-(4-methylbenzylidine)camphor (4mbc) is uterotrophic in immature rats when administered by either subcutaneous injection or oral gavage. these data confirm earlier reports of uterotrophic activity for this agent when administered to immature rats in the diet or by whole-body immersion; however, they are in contrast to negative unpublished immature rat uterotrophic assay results. data also indicate that 4mbc binds to isolated rat ut ... | 2002 | 12003759 |
| neuronal calcium sensor-1 binds to regulated secretory organelles and functions in basal and stimulated exocytosis in pc12 cells. | neuronal calcium sensor-1 (ncs-1) and its non-mammalian homologue, frequenin, have been implicated in a spectrum of cellular processes, including regulation of stimulated exocytosis of synaptic vesicles and secretory granules (sgs) in neurons and neuroendocrine cells and regulation of phosphatidylinositol 4-kinase beta activity in yeast. however, apart from these intriguing putative functions, ncs-1 and frequenin are relatively poorly understood. here, the distribution, dynamics and function of ... | 2002 | 12006624 |
| [cloning and analysis of a novel gene encoding n-terminal acetyltransferase subunit]. | n-terminal acetylation is the most common modification in eukaryotic proteins, affecting stability and activity of proteins. nata is one of the n-terminal acetytransferases in yeast. it is composed of two subunits, nat1 and ard1. defect in one of them leads to loss of activity of nata. null mutant of nat1 in yeast exhibits a variety of phenotypes, including depression of a silent mating type locus (hml), failing to enter g(0) in poor nutrient situations and chromosomes instability. based on homo ... | 2002 | 12019451 |
| stereochemical features of the hydrolysis of 9,10-epoxystearic acid catalysed by plant and mammalian epoxide hydrolases. | cis-9,10-epoxystearic acid was used as a tool to probe the active sites of epoxide hydrolases (ehs) of mammalian and plant origin. we have compared the stereochemical features of the hydrolysis of this substrate catalysed by soluble and membrane-bound rat liver ehs, by soluble eh (purified to apparent homogeneity) obtained from maize seedlings or celeriac roots, and by recombinant soybean eh expressed in yeast. plant ehs were found to differ in their enantioselectivity, i.e. their ability to dis ... | 2002 | 12020347 |
| a krüppel-associated box-zinc finger protein, nt2, represses cell-type-specific promoter activity of the alpha 2(xi) collagen gene. | type xi collagen is composed of three chains, alpha 1(xi), alpha 2(xi), and alpha 3(xi), and plays a critical role in the formation of cartilage collagen fibrils and in skeletal morphogenesis. it was previously reported that the -530-bp promoter segment of the alpha 2(xi) collagen gene (col11a2) was sufficient for cartilage-specific expression and that a 24-bp sequence from this segment was able to switch promoter activity from neural tissues to cartilage in transgenic mice when this sequence wa ... | 2002 | 12024037 |
| cdp840. a prototype of a novel class of orally active anti-inflammatory phosphodiesterase 4 inhibitors. | the discovery, synthesis and biological activity of a series of triarylethane phosphodiesterase 4 inhibitors is described. structure-activity relationship studies are presented for cdp840 (29), a potent, chiral, selective inhibitor of pde 4 (ic(50) 4nm). cdp840 is non-emetic in the ferret at 30mgkg(-1) (po), active in models of inflammation and reverses ozone-induced bronchial hyperreactivity in the guinea pig. | 2002 | 12031318 |
| the identification and characterization of a noncontinuous calmodulin-binding site in noninactivating voltage-dependent kcnq potassium channels. | we show here that in a yeast two-hybrid assay calmodulin (cam) interacts with the intracellular c-terminal region of several members of the kcnq family of potassium channels. cam co-immunoprecipitates with kcnq2, kcnq3, or kcnq5 subunits better in the absence than in the presence of ca2+. moreover, in two-hybrid assays where it is possible to detect interactions with apo-cam but not with ca2+-bound calmodulin, we localized the cam-binding site to a region that is predicted to contain two alpha-h ... | 2002 | 12032157 |
| genomic organization, transcript variants and comparative analysis of the human nucleoporin 155 (nup155) gene. | nucleoporin 155 (nup155) is a major component of the nuclear pore complex (npc) involved in cellular nucleo-cytoplasmic transport. we have acquired the complete sequence and interpreted the genomic organization of the nup155 orthologos from human (homo sapiens) and pufferfish (fugu rubripes), which are approximately 80 and 8 kb in length, respectively. the human gene is ubiquitously expressed in many tissues analyzed and has two major transcript variants, resulted from an alternative usage of th ... | 2002 | 12034489 |
| synergistic activation of the rat laminin gamma1 chain promoter by the gut-enriched kruppel-like factor (gklf/klf4) and sp1. | laminin is a multifunctional heterotrimeric protein present in extracellular matrix where it regulates processes that compose tissue architecture including cell differentiation. laminin gamma1 is the most widely expressed laminin chain and its absence causes early lethality in mouse embryos. laminin gamma1 chain gene (lamc1) promoter contains several gc/gt-rich motifs including the bcn-1 element. using the bcn-1 element as a bait in the yeast one-hybrid screen, we cloned the gut-enriched kruppel ... | 2002 | 12034813 |
| ordered recruitment of histone acetyltransferases and the trap/mediator complex to thyroid hormone-responsive promoters in vivo. | transcriptional coactivators implicated in gene activation by the thyroid hormone receptor (tr) include members of the p160/steroid receptor coactivator (src) family of proteins, p300, and the multisubunit tr-associated protein (trap)/mediator complex. we investigated the temporal recruitment of these cofactors to mammalian thyroid hormone (t3)-responsive promoters in vivo. we show that upon t3 treatment, tr recruits all three types of coactivators to specific promoters in at least two sequentia ... | 2002 | 12034878 |
| interaction of sap97 with minus-end-directed actin motor myosin vi. implications for ampa receptor trafficking. | sap97 is a modular protein composed of three pdz domains, an sh3 domain, and a guanylate kinase-like domain. it has been implicated functionally in the assembly and structural stability of synaptic junctions as well as in the trafficking, recruitment, and localization of specific ion channels and neurotransmitter receptors. the n terminus of sap97 (s97n) has been shown to play a key role in the selection of binding partners and the localization of sap97 at adhesion sites, as well as the clusteri ... | 2002 | 12050163 |
| probing the membrane topology of a subunit of the mitochondrial protein translocase, tim44, with biotin maleimide. | tim44 is an essential component of the translocase of the inner mitochondrial membrane (tim) complex that mediates transport of nuclear encoded mitochondrial precursors across the inner membrane. here, we have investigated the topology of tim44 by probing mitochondria with membrane impermeable 3-(n-maleimidopropionyl)biocytin (mpb) followed by the specific immunoprecipitation of modified proteins. our data indicate that a single cysteine residue, cys-369, located in the c-terminal domain of the ... | 2002 | 12054602 |
| protein import and processing reconstituted with isolated rat liver mitochondria and recombinant mitochondrial processing peptidase. | most mitochondrial proteins are synthesized in the cytoplasm as larger precursors carrying n-terminal matrix-targeting presequences, and are subsequently transported to the mitochondria. the presequence mediates the interaction between the precursor polypeptide and components of the mitochondrial protein import machinery, a complex apparatus that is responsible for translocation of the precursor across the two mitochondrial membranes. once the precursor has reached the mitochondrial matrix, the ... | 2002 | 12054920 |
| rat zinc-fingers and homeoboxes 1 (zhx1), a nuclear factor-ya-interacting nuclear protein, forms a homodimer. | zinc-fingers and homeoboxes 1 (zhx1) is a protein which interacts with the activation domain of the a subunit of nuclear factor-y. to analyze the physiological role(s) of zhx1, we searched zhx1-interacting protein(s) using a yeast two-hybrid system. the rat counterpart of zhx1 cdnas was cloned from an ovarian granulosa cell complementary dna (cdna) library, indicating that zhx1 is able to form a homodimer. an analysis of the nucleotide sequence and its deduced amino acid sequence show that rat z ... | 2002 | 12062805 |
| implication of alpha4 phosphoprotein and the rapamycin-sensitive mammalian target-of-rapamycin pathway in prolactin receptor signalling. | a prolactin (prl)-responsive 3'-end cdna encoding rat alpha4 phosphoprotein was previously isolated from a rat lymphoma cdna library. rat alpha4 is a homologue of yeast tap42 and is a component of the mammalian target-of-rapamycin (mtor) signalling pathway that stimulates translation initiation and g1 progression in response to nutrients and growth factors. in the present study, the full-length rat alpha4 cdna was obtained by 5'-race and the 1023 bp open reading frame predicted a 340 amino acid ... | 2002 | 12065239 |
| brain-derived neurotrophic factor promotes interaction of the nck2 adaptor protein with the trkb tyrosine kinase receptor. | brain-derived neurotrophic factor (bdnf) binds to and activates the trkb tyrosine kinase receptor to regulate cell differentiation, survival, and neural plasticity in the nervous system. however, the identities of the downstream signaling proteins involved in this process remain unclear. using a yeast two-hybrid screen with the intracellular domain (icd-trkb) of the trkb bdnf receptor, we identified the nck2 adaptor protein as a novel interaction partner of the active form of trkb. additionally, ... | 2002 | 12074588 |
| ras family therapy: rab, rho and ral talk to the exocyst. | 2002 | 12074877 | |
| masses of phosphorylated neurofilaments are associated with abnormal golgi apparatus of anterior horn neurons of beta, beta'-iminodipropionitrile-intoxicated rats. | the golgi apparatus (ga) of anterior horn neurons of rats chronically intoxicated with beta,beta'-iminodipropionitrile (idpn) in drinking water was examined with an organelle-specific antibody. the neuropile of the anterior horns contained the typical axonal spheroids associated with idpn toxicity while the perikarya of approximately one-third of the neurons contained phosphorylated neurofilaments, which are not found in the perikarya of control rat neurons. by serial or double immunostaining wi ... | 2002 | 12075937 |
| characterization of saccharomyces cerevisiae acyl-protein thioesterase 1, the enzyme responsible for g protein alpha subunit deacylation in vivo. | thioacylation is a reversible lipid modification of proteins that plays a role in the regulation of signal transduction. acyl-protein thioesterase 1 (apt1) was identified as an enzyme capable of deacylating some thioacylated proteins in vitro. saccharomyces cerevisiae open reading frame ylr118c encodes an enzyme homologous to rattus norvegicus apt1. we demonstrate that the catalytic activity of the protein encoded by the yeast open reading frame is similar to that of rat apt1, and we designate t ... | 2002 | 12080046 |
| a novel natural product compound enhances camp-regulated chloride conductance of cells expressing cftr[delta]f508. | cystic fibrosis (cf) results from mutations in the cystic fibrosis transmembrane conductance regulator (cftr) gene, which encodes a chloride channel localized at the plasma membrane of diverse epithelia. the most common mutation leading to cf, delta f508, occurs in the first nucleotide-binding domain (nbd1) of cftr. the delta f508 mutation disrupts protein processing, leading to a decreased level of mutant channels at the plasma membrane and reduced transepithelial chloride permeability. partial ... | 2002 | 12080183 |
| ptprj is a candidate for the mouse colon-cancer susceptibility locus scc1 and is frequently deleted in human cancers. | only a small proportion of cancers result from familial cancer syndromes with mendelian inheritance. nonfamilial, 'sporadic' cancers, which represent most cancer cases, also have a significant hereditary component, but the genes involved have low penetrance and are extremely difficult to detect. therefore, mapping and cloning of quantitative trait loci (qtls) for cancer susceptibility in animals could help identify homologous genes in humans. several cancer-susceptibility qtls have been mapped i ... | 2002 | 12089527 |
| coordinate regulation of udp-glucuronosyltransferase ugt1a6 induction by 3-methylcholanthrene and multidrug resistance protein mrp2 expression by dexamethasone in primary rat hepatocytes. | concentration-dependent regulation of 3-methylcholanthrene (mc) inducibility of udp-glucuronosyltransferase ugt1a6 by the synthetic glucocorticoid, dexamethasone (dex) was studied. treatment of cultured rat hepatocytes with mc, 0.1, 1, and 10 microm dex, and mc combined with dex, resulted in different induction patterns measured in the intact cells compared to that observed in the microsomes prepared from the same cells. dex treatment in various concentrations caused a concentration-dependent in ... | 2002 | 12110372 |
| mutations in the nucleotide binding domain 1 signature motif region rescue processing and functional defects of cystic fibrosis transmembrane conductance regulator delta f508. | the gene encoding the cystic fibrosis transmembrane conductance regulator (cftr), an atp binding cassette (abc) transporter that functions as a phosphorylation- and nucleotide-regulated chloride channel, is mutated in cystic fibrosis (cf) patients. deletion of a phenylalanine at amino acid position 508 (deltaf508) in the first nucleotide binding domain (nbd1) is the most prevalent cf-causing mutation and results in defective protein processing and reduced cftr function, leading to chloride imper ... | 2002 | 12110684 |
| dehydroepiandrosterone (dhea) metabolism in saccharomyces cerevisiae expressing mammalian steroid hydroxylase cyp7b: ayr1p and fox2p display 17beta-hydroxysteroid dehydrogenase activity. | we have engineered recombinant yeast to perform stereospecific hydroxylation of dehydroepiandrosterone (dhea). this mammalian pro-hormone promotes brain and immune function; hydroxylation at the 7alpha position by p450 cyp7b is the major pathway of metabolic activation. we have sought to activate dhea via yeast expression of rat cyp7b enzyme. saccharomyces cerevisiae was found to metabolize dhea by 3beta-acetylation; this was abolished by mutation at atf2. dhea was also toxic, blocking tryptopha ... | 2002 | 12112241 |
| mammalian mediator subunit mmed8 is an elongin bc-interacting protein that can assemble with cul2 and rbx1 to reconstitute a ubiquitin ligase. | the heterodimeric elongin bc complex has been shown to interact in vitro and in cells with a conserved bc-box motif found in an increasing number of proteins including rna polymerase ii elongation factor elongin a, suppressor of cytokine signaling (socs)-box proteins, and the von hippel-lindau tumor suppressor protein. recently, the elongin bc complex was found to function as an adaptor that links these bc-box proteins to a module composed of cullin family members cul2 or cul5 and ring-h2 finger ... | 2002 | 12149480 |
| postsynaptic targeting of alternative postsynaptic density-95 isoforms by distinct mechanisms. | members of the postsynaptic density-95 (psd95)/synapse-associated protein-90 (sap90) family of scaffolding proteins contain a common set of modular protein interaction motifs including pdz (postsynaptic density-95/discs large/zona occludens-1), src homology 3, and guanylate kinase domains, which regulate signaling and plasticity at excitatory synapses. we report that n-terminal alternative splicing of psd95 generates an isoform, psd95beta that contains an additional "l27" motif, which is also pr ... | 2002 | 12151521 |
| expression of rat multidrug resistance protein 2 (mrp2) in male and female rats during normal and pregnenolone-16alpha-carbonitrile (pcn)-induced postnatal ontogeny. | the normal maturation of biliary organic anion excretion in newborn rats can be enhanced by microsomal enzyme-inducing chemical treatment, yet the mechanism for this phenomenon is not known. multidrug resistance protein 2 (mrp2) is a biliary efflux transporter that is inducible by select microsomal enzyme-inducing chemicals. thus, the aims of this study were to compare the normal and pregnenolone-16alpha-carbonitrile (pcn)-induced postnatal ontogeny of mrp2 in male and female rats. mrp2 protein ... | 2002 | 12167307 |
| cloning and sequencing of cdna encoding the diazepam binding inhibitor (dbi). | the anti-morphine peptide ppc from dog brain has an inhibitory effect on morphine analgesia. it is highly homologous to dbi. the analgesic effect of bovine dbi was measured, showing the potentiation effect on morphine analgesia. in order to study the relationship between ppc and dbi, cdna library derived from dog liver had been screened using partial gene of rat dbi as a probe. a positive clone was obtained. the entire sequence of the insert of this positive clone provided complete sequence of a ... | 1998 | 12168001 |
| the multiple lim domain-containing adaptor protein hic-5 synaptically colocalizes and interacts with the dopamine transporter. | the na+/cl--dependent dopamine transporter (dat) is critical in terminating dopaminergic transmission by removing the transmitter away from the synapse. several lines of evidence suggest that transporter-interacting proteins may play a role in dat function and regulation. in this report, using the yeast two-hybrid system, we have identified a novel interaction between dat and the multiple lin-11, isl-1, and mec-3 (lim) domain-containing adaptor protein hic-5. this association involves the n-term ... | 2002 | 12177201 |
| evidence of functional modulation of the mekk/jnk/cjun signaling cascade by the low density lipoprotein receptor-related protein (lrp). | lipoprotein receptors, such as lrp, have been shown to assemble multiprotein complexes containing intracellular signaling molecules; however, in vivo, their signaling function is poorly understood. using a novel lrp receptor fusion construct, a type i transmembrane protein chimera, termed sigg-lrp (bearing the intracellular cooh-terminal tail of human lrp as recombinant fusion to a transmembrane region plus the extracellular igg-f(c) domain), we here investigated lrp signal transduction specific ... | 2002 | 12193592 |
| intracellular single-chain variable fragments directed to the src homology 2 domains of syk partially inhibit fc epsilon ri signaling in the rbl-2h3 cell line. | intracellular expression of ab fragments has been efficiently used to inactivate therapeutic targets, oncogene products, and to induce viral resistance in plants. ab fragments expressed in the appropriate cell compartment may also help to elucidate the functions of a protein of interest. we report in this study the successful targeting of the protein tyrosine kinase syk in the rbl-2h3 rat basophilic leukemia cell line. we isolated from a phage display library human single-chain variable fragment ... | 2002 | 12193692 |
| the calcium sensor protein visinin-like protein-1 modulates the surface expression and agonist sensitivity of the alpha 4beta 2 nicotinic acetylcholine receptor. | the calcium sensor protein visinin-like protein-1 (vilip-1) was isolated from a brain cdna yeast two-hybrid library using the large cytoplasmic domain of the alpha4 subunit as a bait. vilip-1 is a myristoylated calcium sensor protein that contains three functional calcium binding ef-hand motifs. the alpha4 subunit residues 302-339 were found to be essential for the interaction with vilip-1. vilip-1 coimmunopurified with detergent-solubilized recombinant alpha4beta2 acetylcholine receptors (achrs ... | 2002 | 12202488 |
| kif1bbeta2, capable of interacting with chp, is localized to synaptic vesicles. | kinesin family proteins are microtubule-dependent molecular motors involved in the intracellular motile process. using a ca2+ -binding protein, chp (calcineurin b homologous protein), as a bait for yeast two hybrid screening, we identified a novel kinesin-related protein, kif1bbeta2. kif1bbeta2 is a member of the kif1 subfamily of kinesin-related proteins, and consists of an amino terminal kif1b-type motor domain followed by a tail region highly similar to that of kif1a. chp binds to regions adj ... | 2002 | 12204119 |
| evidence of a novel dipeptidyl aminopeptidase in mammalian gh(3) cells: new insights into the processing of peptide hormone precursors. | we investigated whether yeast signals could regulate hormone processing in mammalian cells. chmeric genes coding for the prepro region of yeast alpha-factor and the functional hormone region of anglerfish somatostatin was expressed in rat pituitary gh(3) cells. the nascent prepro-alpha-factor-somatostatin peptides disappeared from cells with a half-life of 30 min, and about 20% of unprocessed precursors remained intracellular after a 2 h chase period. disappearance of propeptide was insensitive ... | 2002 | 12207045 |
| glucose down-regulates per1 and per2 mrna levels and induces circadian gene expression in cultured rat-1 fibroblasts. | in mammals, peripheral circadian clocks are present in most tissues, but little is known about how these clocks are synchronized with the ambient 24-h cycles. by using rat-1 fibroblasts, a model cell system of the peripheral clock, we found that an exchange of the culture medium triggered circadian gene expression that was preceded by slow down-regulation of per1 and per2 mrna levels. this profile contrasts to the immediate up-regulation of these genes often observed for clock resetting. the scr ... | 2002 | 12213820 |
| a rab8-specific gdp/gtp exchange factor is involved in actin remodeling and polarized membrane transport. | the mechanisms mediating polarized delivery of vesicles to cell surface domains are poorly understood in animal cells. we have previously shown that expression of rab8 promotes the formation of new cell surface domains through reorganization of actin and microtubules. to unravel the function of rab8, we used the yeast two-hybrid system to search for potential rab8-specific activators. we identified a coil-coiled protein (rabin8), homologous to the rat rabin3 that stimulated nucleotide exchange o ... | 2002 | 12221131 |
| the human homolog of the rat inositol phosphate multikinase is an inositol 1,3,4,6-tetrakisphosphate 5-kinase. | we have demonstrated that the human homolog of the rat inositol phosphate multikinase is an inositol 1,3,4,6-tetrakisphosphate 5-kinase (insp(4) 5-kinase). the cdna of the human gene contained a putative open reading frame of 1251 bp encoding 416 amino acids with 83.6% identity compared with the rat protein. the substrate specificity of the recombinant human protein demonstrated preference for ins(1,3,4,6)p(4) with a catalytic efficiency (v(max)/k(m)) 43-fold greater than that of ins(1,3,4,5)p(4 ... | 2002 | 12223481 |
| candida-specific antibodies during experimental vaginal candidiasis in mice. | protective host defense mechanisms against vaginal candida albicans infections are poorly understood. although cell-mediated immunity (cmi) is the predominant host defense mechanism against most mucosal candida infections, the role of cmi against vaginal candidiasis is uncertain, both in humans and in an experimental mouse model. the role of humoral immunity is equally unclear. while clinical observations suggest a minimal role for antibodies against vaginal candidiasis, an experimental rat mode ... | 2002 | 12228309 |
| complementary dna cloning and characterization of cytochrome p450 2d29 from japanese monkey liver. | a cdna was cloned from japanese monkey liver mrna by reverse transcriptase-polymerase chain reaction (rt-pcr) using oligonucleotide primers based on the marmoset cytochrome p450 2d19 (cyp2d19) nucleotide sequence. the full-length cdna encoded a 497 amino acid protein (designated cyp2d29) that is 96, 91, and 88% homologous to human cyp2d6, cynomolgus monkey cyp2d17, and marmoset monkey cyp2d19, respectively. yeast cells (saccharomyces cerevisiae ah-22 strain) transfected with pgyr1 vectors contai ... | 2002 | 12234613 |
| neuronal calcium sensor 1 and phosphatidylinositol 4-oh kinase beta interact in neuronal cells and are translocated to membranes during nucleotide-evoked exocytosis. | neuronal calcium sensor 1 (ncs-1) belongs to a family of ef-hand calcium-binding proteins and is mainly expressed in neurons and neuroendocrine cells, where it causes facilitation of neurotransmitter release through unknown mechanisms. the yeast homologue of ncs-1 has been demonstrated to interact with and regulate the activity of yeast phosphatidylinositol 4-oh kinase beta (pi4kbeta). however, in neurons and neurosecretory cells ncs-1 has not unequivocally been shown to interact with pi4kbeta. ... | 2002 | 12244129 |
| characterization of n-linked oligosaccharides attached to recombinant human antithrombin expressed in the yeast pichia pastoris. | we studied the structures of four n-linked oligosaccharide chains of the recombinant human antithrombin (rat) expressed in the yeast pichia pastoris. rat was fully glycosylated at asn 96 and asn 155, whereas the glycosylation on asn 135 and asn 192 was partial. the glycosylation level on asn 135 was only 12% and this reduction is assumed to be one of the reasons for a higher heparin-binding affinity of rat than plasma-derived human antithrombin (pat). in order to determine the sizes and electros ... | 2002 | 12271456 |
| dietary fiber suppresses elevations of uric acid and allantoin in serum and urine induced by dietary rna and increases its excretion to feces in rats. | this study was performed to examine the effects of several kinds of dietary fibers (df) with different physical properties on dietary rna metabolism. male wistar strain rats, 4 wk old, were fed diets with or without a 3% yeast rna and a 5% df (cellulose, chitin, chitosan, inulin, and xanthan gum) for 20 d (experiment 1) or 5 d (experiment 2). feeding df tested lowered the serum uric acid and allantoin concentrations and the urinary excretions of their compounds and increased the amount of rna ex ... | 2002 | 12350076 |
| iron absorption in rats increased by yeast glucan. | the effects of brewer's yeast cell walls and two of its components, glucan and mannan, on the absorption of 59fe by anemic rats were investigated. after administration of the label, the percentage of 59fe taken up into the blood of group given glucan was generally similar to that of a group given yeast cell walls, both values were higher than in controls. the incorporation of 59fe into the small intestines was higher in the group given glucan than in the controls or a group given a glucan-mannan ... | 2002 | 12353639 |
| trafficking of the vesicular acetylcholine transporter in sn56 cells: a dynamin-sensitive step and interaction with the ap-2 adaptor complex. | the pathways by which synaptic vesicle proteins reach their destination are not completely defined. here we investigated the traffic of a green fluorescent protein (gfp)-tagged version of the vesicular acetylcholine transporter (vacht) in cholinergic sn56 cells, a model system for neuronal processing of this cargo. gfp-vacht accumulates in small vesicular compartments in varicosities, but perturbation of endocytosis with a dominant negative mutant of dynamin i-k44a impaired gfp-vacht trafficking ... | 2002 | 12358769 |
| freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. | biological membranes are often poorly visible with the electron microscope after high-pressure freezing and freeze-substitution. the water content of the sample and of the substitution medium is one factor among others that strongly influences membrane visibility. in order to investigate this effect, high-pressure frozen yeast cells, rat-pancreas tissue and arthropod tissue were freeze-substituted with and without adding water to the substitution medium. the visibility of the biological membrane ... | 2002 | 12366592 |
| sharp is a novel component of the notch/rbp-jkappa signalling pathway. | notch proteins are the receptors for an evolutionarily highly conserved signalling pathway that regulates numerous cell fate decisions during development. signal transduction involves the presenilin-dependent intracellular processing of notch and nuclear translocation of the intracellular domain of notch, notch-ic. notch-ic associates with the dna-binding protein rbp-jkappa/cbf-1 to activate transcription of notch target genes. in the absence of notch signalling, rbp-jkappa/cbf-1 acts as a trans ... | 2002 | 12374742 |
| the evolutionary and integrative roles of transthyretin in thyroid hormone homeostasis. | in larger mammals, thyroid hormone-binding plasma proteins are albumin, transthyretin (ttr) and thyroxine (t4)-binding globulin. they differ characteristically in affinities and release rates for t4 and triiodothyronine (t3). together, they form a 'buffering' system counteracting thyroid hormone permeation from aqueous to lipid phases. evolution led to important differences in the expression pattern of these three proteins in tissues. in adult liver, ttr is only made in eutherians and herbivorou ... | 2002 | 12379491 |
| detection of c8-(1-hydroxyethyl)guanine in liver rna and dna from control and ethanol-treated rats. | alcohol consumption is associated with an increased risk of cancer by mechanisms that remain unknown but have been suggested to involve radical metabolites. we have previously shown that the 1-hydroxyethyl radical produced from ethanol oxidation in vitro is able to alkylate nucleic acids to produce c8-(1-hydroxyethyl)guanine [c8-(1-he)gua] among other products. to assess if this adduct is produced in vivo, we developed a sensitive hplc-ms/ms method for its detection and analyzed hydrolysates of ... | 2002 | 12387621 |
| densin-180, a synaptic protein, links to psd-95 through its direct interaction with maguin-1. | densin-180, a brain-specific protein highly concentrated at the postsynaptic density (psd), belongs to the lap [leucine-rich repeats and psd-95/dlg-a/zo-1 (pdz) domains] family of proteins, some of which play fundamental roles in the establishment of cell polarity. | 2002 | 12390249 |
| filamin a and filamin b are co-expressed within neurons during periods of neuronal migration and can physically interact. | mutations in the x-linked gene filamin a (flna) lead to the human neurological disorder, periventricular heterotopia (ph). although ph is characterized by a failure in neuronal migration into the cerebral cortex with consequent formation of nodules in the ventricular and subventricular zones, many neurons appear to migrate normally, even in males, suggesting compensatory mechanisms. here we characterize expression patterns for flna and a highly homologous protein filamin b (flnb) within the nerv ... | 2002 | 12393796 |
| recombinant human heparin-binding neurite-promoting factor expressed with yeast stimulates neurites outgrowth. | heparin-binding neurite-promoting factor (hbnf) is a heparin-binding protein primarily found in the brain, which can stimulate neurite outgrowth in vitro. we expressed recombinant human heparin-binding neurite-promoting factor (hrhbnf) using a yeast system, and observed its activity in stimulating neurite outgrowth in vitro. | 2002 | 12411111 |
| vigilant vectors: adeno-associated virus with a biosensor to switch on amplified therapeutic genes in specific tissues in life-threatening diseases. | there are many life-threatening and chronic diseases in which physiological signals could be used to switch on therapeutic protective genes. we are developing a gene therapy approach in which a systemically injected "vigilant vector" waits for these signals and switches on genes to protect specific tissues with high amplification. the concept of a vigilant vector requires four components. the first component is a safe and stable vector that can be administered by systemic injection and express t ... | 2002 | 12413425 |
| thyroid hormone action is disrupted by bisphenol a as an antagonist. | bisphenol a (bpa), a monomer of polycarbonate plastics, has been shown to possess estrogenic properties and act as an agonist for the estrogen receptors. although an epidemiologically based investigation has suggested that some chemicals could disrupt thyroid function in animals, the effects on thyroid hormone receptors (trs) are unknown. we show here that bpa inhibits tr-mediated transcription by acting as an antagonist. in the transient gene expression experiments, bpa suppressed transcription ... | 2002 | 12414890 |
| expression and genomic organization of the zebrafish chaperonin gene complex. | chaperonin 10 and chaperonin 60 monomers exist within the multimeric mitochondrial chaperonin folding complex with a stoichiometry of 2:1. this complex is located in the mitochondrial matrix, where it aids in the folding and acquisition of the tertiary structure of proteins. we have previously isolated the cpn10 cdna in zebrafish (danio rerio), and demonstrated that it is ubiquitously expressed during embryonic development and transcriptionally upregulated after exposure to heat shock. in the pr ... | 2002 | 12416612 |
| evidence for the presence of habp1 pseudogene in multiple locations of mammalian genome. | the gene encoding hyaluronan-binding protein 1 (habp1) is expressed ubiquitously in different rat tissues, and is present in eukaryotic species from yeast to humans. fluorescence in situ hybridization indicates that this is localized in human chromosome 17p13.3. here, we report the presence of homologous sequences of habp1 cdna, termed processed habp1 pseudogene in humans. this is concluded from an additional pcr product of ~0.5 kb, along with the expected band at approximately 5 kb as observed ... | 2002 | 12443542 |
| identification of a non-canonical e-box motif as a regulatory element in the proximal promoter region of the apolipoprotein e gene. | we have used the yeast one-hybrid system to identify transcription factors with binding capability to specific sequences in proximal regions of the apolipoprotein e gene ( apoe ) promoter. the sequence between -113 and -80 nt, which contains regulatory elements in various cell types, was used as a bait to screen a human brain cdna library. four cdna clones that encoded portions of the human upstream-stimulatory-factor (usf) transcription factor were isolated. electrophoretic-mobility-shift assay ... | 2003 | 12444925 |
| pneumocystis. | pneumocystis organisms can cause pneumonia in mammals that lack a strong immune defense. the genus pneumocystis contains many different organisms that can be distinguished by dna sequence analysis. these different organisms are different species of yeast-like fungi that are most closely related to the ascomycete, schizosaccharomyces pombe. each species of pneumocystis appears to be specific for the mammal in which it is found. the species that infects humans is pneumocystis jiroveci. p. jiroveci ... | 2002 | 12452285 |
| dual mechanism of signal transducer and activator of transcription 5 activation by the insulin receptor. | insulin stimulates signal transducer and activator of transcription 5 (stat5) activation in insulin receptor (ir)-overexpressing cell lines and in insulin target tissues of mice. stat5b and insulin receptor substrate 1 (irs-1) interact with the same autophosphorylation site in the ir [phosphotyrosine (py) 972] in yeast two-hybrid assays, and the ir phosphorylates stat5b in vitro. these data suggest that stat5 proteins might be recruited to, and phosphorylated by, the activated ir in vivo. nevert ... | 2002 | 12456798 |
| molecular cloning and characterization of protein phosphatase 2c of vomeronasal sensory epithelium of garter snakes. | the earthworm-derived chemoattractant es20 interacts with its g-protein-coupled receptors on the plasma membrane of vomeronasal (vn) sensory neurons of garter snakes, resulting in an increase in inositol trisphosphate [j. biol. chem. 269 (1994) 16867] and a rapid phosphorylation of the membrane-bound proteins, p42/44 [biochim. biophys. acta 1450 (1999) 320]. the phosphorylation of p42/44 proteins are countervailingly regulated by a protein kinase and an okadaic acid-insensitive but fluoride-sens ... | 2002 | 12464270 |
| functional domains involved in the interaction between orc1 and transcriptional repressor alf-c that bind to an origin/promoter of the rat aldolase b gene. | the promoter of the rat aldolase b (aldb) gene functions in vivo as an origin of dna replication in the cells in which transcription of the gene is repressed. previously, we identified two closely related dna-binding proteins, alf-c1 and alf-c2, which repressed the aldb gene promoter. we also reported that the binding site of these proteins, site c, is one of the required dna elements of the aldb gene origin/promoter for autonomously replicating activity in transfected cells. in the present stud ... | 2002 | 12466545 |
| topoisomerase i and ii enzyme inhibitory aqueous extract of ardisia compressa and ardisin protect against benomyl oxidation of hepatocytes. | tea preparations of ardisia compressa (ac) have been used in folk medicine against liver disorders. the objective of this study was to evaluate the in vitro topoisomerase i and ii enzyme inhibition and the antioxidant effect of an aqueous extract from dry leaves of ac and a pure component (ardisin) purified from ac on benomyl (be)-induced cytotoxicity in primary culture rat hepatocytes. lipid peroxidation (malondialdehyde), antioxidant enzyme activities of glutathione reductase, glutathione pero ... | 2002 | 12475294 |
| substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein ucp1 lead to the reduction of fatty acid-induced h+ uniport. | mutants were constructed for mitochondrial uncoupling protein ucp1, with single or multiple substitutions within or nearby the ucp-signatures located in the first alpha-helix and second matrix-segment, using the quickchange site directed mutagenesis protocol (stratagene), and were assayed fluorometrically for kinetics of fatty acid (fa)-induced h+ uniport and for cl- uniport. their ability to bind 3h-gtp was also evaluated. the wild type ucp1 was associated with the fa-induced h+ uniport proport ... | 2003 | 12479871 |
| a dominant negative form of the aaa atpase skd1/vps4 impairs membrane trafficking out of endosomal/lysosomal compartments: class e vps phenotype in mammalian cells. | skd1 is a member of the family of atpases associated with cellular activities whose yeast homologue vps4p has been implicated in endosomal/vacuolar membrane transports. when a mutant of skd1 that lacks atpase activity [skd1(e235q)] was overexpressed in mammalian cells, it induced a dominant negative phenotype characterized by aberrant endosomal structures (denoted as e235q compartments). expression of skd1(e235q) caused an accumulation of basolateral recycling receptors, such as asialoglycoprote ... | 2003 | 12482925 |
| hepatobiliary transport of bile acids and organic anions. | recent studies have elucidated the mechanism and regulation of hepatic transport of bile acids and organic anions. bile acids are taken up into hepatocytes by basolateral transporters, na+-dependently by na+/taurocholate cotransporting polypeptide (ntcp) and na+-independently by organic anion transporting polypeptide (oatp) families. organic anions are taken up into hepatocytes by oatp families. these compounds are then transported in hepatocytes bound to cytosolic binders, and subjected to tran ... | 2002 | 12483266 |
| comparison of ingestive effects of brewer's yeast, casein, and soy protein on bioavailability of dietary iron. | the effects of brewer's yeast, casein, and soy protein intakes on the absorption and retention as well as the incorporation into hemoglobin and systemic iron stores of dietary iron were examined in an animal experiment with growing rats. relative biological values (rbv) of iron in the rats fed casein (c), soy protein (sp), and yeast (y) diets were 1.00, 0.31, and 1.77, respectively. the apparent absorption of iron in y-diet-fed rats was significantly higher than that in c- or sp-diet-fed rats. t ... | 2002 | 12489821 |
| trapping and characterization of novel retinoid response elements. | retinoids, such as retinoic acid (ra), play a critical role in normal vertebrate development and physiology. however, embryonic exposure to excess retinoids also causes severe malformations. retinoids bind ra receptors and retinoid x receptors, thus activating a plethora of genes. separating the genes induced directly by retinoid-bound receptors from those induced subsequently by other transcription factors is difficult. the loose consensus defining known ra responsive elements (rares) further c ... | 2003 | 12511604 |
| intracellular trafficking and metabolic turnover of yeast prepro-alpha-factor-srif precursors in gh3 cells. | chimeric genes coding for prepro region of yeast alpha-factor and anglerfish srif were expressed in rat gh3 cells to determine whether yeast signals could regulate hormone processing in mammalian cells. we report that nascent hybrid polypeptides were efficiently targeted to er, where cleavage of signal peptides and core glycosylation occurred, and were localized mainly in golgi. these data indicate that prepro region of yeast alpha-factor functions in sorting molecules to secretory pathway in ma ... | 2002 | 12515394 |
| molecular chaperones hsp90 and hsp70 deliver preproteins to the mitochondrial import receptor tom70. | the role of cytosolic factors in protein targeting to mitochondria is poorly understood. here, we show that in mammals, the cytosolic chaperones hsp90 and hsp70 dock onto a specialized tpr domain in the import receptor tom70 at the outer mitochondrial membrane. this interaction serves to deliver a set of preproteins to the receptor for subsequent membrane translocation dependent on the hsp90 atpase. disruption of the chaperone/tom70 recognition inhibits the import of these preproteins into mitoc ... | 2003 | 12526792 |
| ras-mediated apoptosis of pc cl 3 rat thyroid cells induced by ret/ptc oncogenes. | ret gene rearrangements, which generate chimeric ret/ptc oncogenes, are early events in the evolution of thyroid papillary carcinomas. expression of ret/ptc oncogenes promotes neoplastic transformation of cultured thyroid cells and of thyroid glands in transgenic mice. notwithstanding these oncogenic effects, we have found that the expression of two ret/ptc oncogenes (h4-ret and rfg-ret) induces apoptosis of rat thyroid pc cl 3 cells. promotion of thyroid cell death depends on the kinase activit ... | 2003 | 12527893 |
| an integrated physical and gene map of the 3.5-mb chromosome 3p21.3 (ap20) region implicated in major human epithelial malignancies. | to facilitate the identification of tumor suppressor genes in the chromosome 3p21.3-p22 ap20 subregion, we constructed a 3.5-mb physical and gene map of this segment (between markers d3s4285 and d3s3873) that spans the distance from 124.4cr3000 to 133.5 cr3000 of the gb4 genetic map. we used noti-linking and -jumping clones, sequence-tagged site pcr marker analysis, and multicolor and fiber fluorescence in situ hybridization to confirm the sequence order and map orientation. an integrated clone ... | 2003 | 12543795 |
| down-regulation of the na+/taurocholate cotransporting polypeptide during pregnancy in the rat. | experimental studies have shown decreased bile acid (ba) uptake and reduced excretion of cholephilic compounds in pregnant rodents. | 2003 | 12547402 |
| direct binding of syndecan-4 cytoplasmic domain to the catalytic domain of protein kinase c alpha (pkc alpha) increases focal adhesion localization of pkc alpha. | syndecan-4 is a transmembrane heparan sulfate proteoglycan that acts as a coreceptor with integrins in focal adhesion formation. the central region of syndecan-4 cytoplasmic domain (4v; lgkkpiykk) binds phosphatidylinositol 4,5-bisphosphate, and together they regulate protein kinase c alpha (pkc alpha) activity. syndecan 4v peptide directly potentiates pkc alpha activity, leading to "superactivation" of the enzyme, apparently through an interaction with its catalytic domain. we now have performe ... | 2003 | 12571249 |
| [pharmacological studies of trilex on treatment of pharyngitis]. | trilex consisted of three species: ilex latifolia, ilex asprella and ilex rotunda. trilex had the antipyretic effects, decreased the rat body temperature about 0.8 degree c on yeast induced hyperthermia. trilex could obviously enhance the threshold of hot plate induced pain in mice. the maximum threshold increased 43.8%. trilex could also inhibit acetic acid induced inflammatory ooze in mice abdominal cavity. the inhibitory rate was 34.8%. these results showed that trilex had antipyretic, analge ... | 2000 | 12575042 |
| evaluation of antipyretic activity of leaf extracts of mallotus peltatus (geist) muell. arg. var acuminatus: a folk medicine. | a study was carried out to evaluate the anti-pyretic potential of the methanol extract of mallotus peltatus (geist) muell. arg. var acuminatus leaf, a folk medicine of onge tribes of bay islands, on normal body temperature and yeast-induced pyrexia in wister albino rats. the leaf extract at oral doses of 100, 200 and 300 mg kg(-1), p.o., showed significant reduction in normal body temperature and yeast-provoked elevated temperature in a dose-dependent manner and the anti-pyretic effect was compa ... | 2002 | 12587693 |
| evaluation of anti-pyretic potential of ficus racemosa bark. | a study was carried out to evaluate the anti-pyretic effect of a methanol extract of stem bark of ficus racemosa linn. (mefr) on normal body temperature and yeast-induced pyrexia in albino rats. a yeast suspension (10 ml/kg body wt.) increased rectal temperature 19 h after subcutaneous injection. the mefr, at doses of 100, 200 and 300 mg/kg body wt. p.o., showed significant dose-dependent reduction in normal body temperature and yeast-provoked elevated temperature. the effect extended up to 5 h ... | 2002 | 12587694 |
| sensory neuron proteins interact with the intracellular domains of sodium channel nav1.8. | voltage-gated sodium channels initiate and propagate action potentials in excitable cells. the tetrodotoxin-resistant na(+) channel (na(v)1.8/sns) is expressed in damage-sensing neurons (nociceptors) and plays an important role in pain pathways. expression of high levels of functional na(v)1.8 in heterologous cells has proved problematic, even in the presence of known sodium channel accessory beta-subunits. this suggests that other regulatory proteins are required for normal levels of na(v)1.8 e ... | 2003 | 12591166 |
| decreased hepatobiliary transport of methotrexate in adjuvant arthritis rats. | 1. we investigated the difference in hepatobiliary transport of methotrexate in normal and adjuvant arthritis (aa) rats and substantiated the expression level of multidrug resistance-associated protein 2 (mrp2) in the liver. 2. biliary clearance of methotrexate in normal and aa rats was calculated from plasma concentrations and biliary excretion following intravenous infusion and hepatic uptake clearance was estimated from an integration plot using methotrexate concentrations in plasma and liver ... | 2002 | 12593762 |
| effects of equilibrium exchange on diffusion-weighted nmr signals: the diffusigraphic "shutter-speed". | a general picture is presented of the implications for diffusion-weighted nmr signals of the parsimonious two-site-exchange (2sx) paradigm. in particular, it is shown that the diffusigraphic "shutter-speed," tau(-1) identical with |q(2)(d(a) - d(b))|, is a useful concept. the "wave-number" q has its standard definition (given in the text), and d(a) and d(b) are the apparent diffusion coefficients (adcs) of molecules in the two "sites," a and b, if there is no exchange between them. at low gradie ... | 2003 | 12594747 |
| novel espin actin-bundling proteins are localized to purkinje cell dendritic spines and bind the src homology 3 adapter protein insulin receptor substrate p53. | we identified a group of actin-binding-bundling proteins that are expressed in cerebellar purkinje cells (pcs) but are not detected in other neurons of the cns. these proteins are novel isoforms of the actin-bundling protein espin that arise through the use of a unique site for transcriptional initiation and differential splicing. light and electron microscopic localization studies demonstrated that these espin isoforms are enriched in the dendritic spines of pcs. they were detected in the head ... | 2003 | 12598619 |