Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| oral contraceptives and cancer. a review of the evidence. | the following review considers epidemiological data published from 1990 onwards on oral contraceptives (ocs) and the risk of cancers of the breast, cervix uteri, endometrium, ovary, liver and skin. in several studies, breast cancer risk was seen to be elevated among women who were current users of an oc, or had recently stopped using an oc, whereas there was no residual risk 5 or more years after stopping oc use. no interaction was observed between type of oc, or with any recognised risk factor ... | 1996 | 8713694 |
| detection of human papillomavirus type 57 in a case of inverted nasal papillomatosis in japan. | human papillomavirus (hpv) type 57 dna was detected in recurrent nasal inverted papillomatosis, in a 60-year-old japanese male, using southern blot hybridization. hpv types 6, 11, 16 and 18 were not detectable in the papilloma. previous studies have shown that hpv types 6, 11, 16 and 18 were mainly found in nasal papillomatosis in other laboratories but the actual detection rate of virus was low. we speculate that this low detection rate may be due to the lack of a method for detecting hpv type ... | 1995 | 8719598 |
| human papillomavirus dna in invasive cervical carcinomas and its association with patient survival: a nested case-control study. | this study sought to examine the association between the presence of human papillomavirus (hpv) dna in invasive cervical cancer and prognosis. a case-control study was undertaken nested in a cohort of 208 patients with invasive cervical carcinoma in montreal. all 40 deceased patients formed the case groups. a control group of equal size was selected by matching to each case (1:1) a patient of the same age and year of admission who had survived her disease. hpv dna was detected by the use of a pc ... | 1996 | 8722218 |
| vulvar squamous cell carcinoma. | squamous cell carcinoma of the vulva is a rare disease, mainly seen in elderly women. risk factors are advanced age, an immunocompromised status, longstanding vulvar dystrophy, vin, a history of vulvar human papillomavirus infection, and a history of cervical cancer. vulvar cancer should be considered as a skin tumor and detection is possible in an early stage. however, because of patients' and doctors' delay, one in three vulvar cancers is not treated before an advanced stage. the tumor metasta ... | 1996 | 8723825 |
| primary keratinocytes can be infected by natural isolates of genital human papillomavirus. | viral particles of human papillomavirus (hpv) types 11 and 16 were isolated from flat condylomas and biopsies of dysplastic lesions and used to infect human keratinocytes. the presence of hpv dna in infected cells was determined by molecular hybridization and the polymerase chain reaction. late transcripts were detected shortly after infection. the persistence of hpv was limited to early passages after infection and the loss of viral dna preceded cell culture senescence. our experimental evidenc ... | 1995 | 8724856 |
| improved detection of human papillomavirus infection in genital intraepithelial neoplasia in human immunodeficiency virus positive (hiv +) women by polymerase chain reaction-in situ hybridization. | the prevalence of genital human papillomavirus (hpv) infection was evaluated in 30 consecutive human immunodeficiency virus (hiv) + women by polymerase chain reaction (pcr)-in situ hybridization (ish) on paraffin-embedded tissue sections and compared with that found with standard ish. biopsies were removed from normal or neoplastic areas in the cervix, vagina, and vulva, and ish was performed with biotinylated or fluorescein isothiocyanate genomic dna probes. one probe was used for hpv screening ... | 1996 | 8727101 |
| hiv-1, hiv-2, human papillomavirus infection and cervical neoplasia in high-risk african women. | to determine the effect of hiv-1 and hiv-2 infection on the prevalence of cervical human papillomavirus (hpv) and squamous intraepithelial lesions (sil) in a population of high-risk women in senegal. | 1996 | 8728046 |
| papillomavirus in cervicovaginal smears of women infected with human immunodeficiency virus. | it has been described that women infected with the human immunodeficiency virus (hiv) present more frequent cytological abnormalities in cervicovaginal smears, generally related to infection by human papillomavirus (hpv). the present work is a study of cervicovaginal smears of 147 hiv-seropositive women submitted to routine gynecological examinations. the smears were stained by the papanicolaou method. cytopathic effects of hpv were found in 38 (25.8%) cases. nuclear atypias of cervical intraepi ... | 1995 | 8731285 |
| [esophageal squamous cell papilloma. report of 3 cases. diagnostic aspects]. | dysphagia is not always present in patients with esophageal squamous cell papilloma, and it can be an incidental finding during gastrointestinal endoscopy of an asymptomatic patient. | 1995 | 8731606 |
| [a case of conjunctival raspberry tumor of viral origin]. | we describe the clinical and histopathological findings of a conjunctival papilloma due to papillomavirus. the therapeutic method applied was excision plus cryotherapy. these lesions are known to recur, and papillomavirus dna sequences are present in several cases of conjunctival dysplasia and carcinoma. using biological molecular methods (in situ hybridization, southern blot hybridization, and particularly polymerase chain reaction), more than 60 human papillomavirus have currently been reporte ... | 1996 | 8731783 |
| herpes simplex virus infection masquerading as condyloma acuminata in a patient with hiv disease. | verrucous lesions in patients with human immunodeficiency virus (hiv) disease may be caused by viruses other than the human papillomavirus. we describe a 32-year-old hiv-positive black man who presented with a verrucous lesion of the intergluteal cleft that clinically resembled condyloma acuminata or verrucous carcinoma. histopathological examination revealed the changes of herpes virus infection, and culture of the tissue confirmed the presence of herpes simplex virus. human papillomavirus was ... | 1996 | 8733396 |
| college health providers' knowledge, attitudes, and management practices of genital hpv infection. | this comparative survey of college health providers explored nurse practitioners' (n = 73) and physicians' (n = 70) knowledge, attitudes, and management practices related to genital human papillomavirus (hpv) infection in females. both groups had adequate knowledge of basic issues of hpv epidemiology, diagnosis, and treatment. divergent attitudes and management practices were reported among the providers, with generally low agreement with national std treatment guidelines published by the center ... | 1996 | 8734630 |
| detection of human papillomavirus type 16 dna in two cases of verrucous carcinoma of the foot. | 1996 | 8736356 | |
| use of the polymerase chain reaction to specifically amplify integrated hpv-16 dna by virtue of its linkage to interspersed repetitive dna. | a polymerase chain reaction (pcr) based technique that combines a virus specific primer and a human interspersed repetitive sequence (irs) specific primer in order to detect integration of human papilloma virus type 16 (hpv-16) is described. amplification of viral-host dna junctions occurs when viral integration results in placement of the virus specific primer binding site near (less that 3-4 kb) the primer binding site within a repetitive sequence element. the method relies on enzyme labeled o ... | 1996 | 8737394 |
| human papilloma virus--h.p.v. condyloma. current studies in diagnosis, treatment and prognosis. | since 1982, in the republic of panama, we evaluated 5,400 women who showed the oncological risk (r.o.) in 59% and in which the participation of pure or associated hpv was 87.6%. the incidence of the hpv in our female population is 52% with a prevailing age of 20-40 years. | 1996 | 8737622 |
| an experimental model of multistep laryngeal carcinogenesis: combined effect of human papillomavirus type 16 genome and n-methyl-n'-nitro-n-nitrosoguanidine. | this report describes the two-step in vitro transformation of human laryngeal epithelial cells (hlec cells). primary cultured hlec cells were first transfected with cloned full-length human papillomavirus type 16 dna, and two immortalized cell lines (hlec-16 cell lines) were selected by subculturing transfected cells that continued to proliferate. the hlec-16 cell lines were not tumorigenic in nude mice, and did not proliferate well in a culture medium containing a physiological level of calcium ... | 1996 | 8740818 |
| p53 expression and human papillomavirus infection in transplant recipients and in patients with epidermodysplasia verruciformis. | 1996 | 8746364 | |
| lack of efficacy of interferon-alpha therapy in recurrent, advanced cervical cancer. | human papillomavirus (hpv) is associated with 65-95% of in situ or early invasive squamous cell carcinomas of the cervix. a multiinstitutional, prospective phase ii clinical trial was initiated by the eastern cooperative oncology group (ecog) to study the activity of ifn-alpha 2b in women with metastatic or locally recurrent cervix cancer. the activity of ifn-alpha 2b was correlated with the presence of hpv as measured by southern blot hybridization or polymerase chain reaction techniques in 17 ... | 1995 | 8746781 |
| presence of human papilloma virus in transitional cell carcinoma in jewish population in israel. | the possible causal association of human papilloma virus (hpv) with transitional cell carcinoma (tcc) of the urinary bladder in israeli jewish patients was assessed. one hundred and ten histopathological tcc sections were examined by peroxidase anti-peroxidase (pap) method. hpv capsid antigen was demonstrated in 19 out of 110 cases (17.3%). hpv-dna sequences, determined by in situ dna-dna hybridization at high stringency wash were present in 24 cases (21.8%): 16(14.5%) cases proved to be hpv6/11 ... | 1995 | 8747082 |
| detection of human papillomavirus dna in primary lung carcinoma by nested polymerase chain reaction. | human papillomaviruses (hpv) have been implicated in the pathogenesis of human squamous cell carcinoma, especially of cervical carcinomas. in two previous studies concerning squamous cell carcinomas of the lung, dna of hpv subtypes 6/11/16/18 (and 31/33/35 for one study) was detected by in situ hybridization in 7% to 30% of the cases. a series of 31 frozen biopsies of lung carcinomas were examined for the presence of hpvdna by nested polymerase chain reaction (pcr). type-specific primers (6/11, ... | 1995 | 8747090 |
| surrogate endpoint biomarkers for cervical cancer chemopreventive trials. | cervical intraepithelial neoplasia (cin) represents a spectrum of epithelial changes that provide an excellent model for developing chemopreventive interventions for cervical cancer. possible drug effect surrogate endpoint biomarkers are dependent on the agent under investigation. published and preliminary clinical reports suggest retinoids and carotenoids are effective chemopreventive agents for cin. determination of plasma and tissue pharmacology of these agents and their metabolites could ser ... | 1995 | 8747385 |
| bladder squamous cell carcinoma with human papilloma virus type 6 [hpv 6]. | a 91-year-old male was diagnosed with squamous cell carcinoma of the urinary bladder. molecular biological examination using the polymerase chain reaction (pcr) method demonstrated the presence of hpv type 6. hpv type 6 is considered very rare in urinary bladder malignancies. | 1995 | 8749958 |
| interleukin 2 production in vitro by peripheral lymphocytes in response to human papillomavirus-derived peptides: correlation with cervical pathology. | human papillomavirus (hpv) is believed to be the major cause of cervical cancer. to investigate whether a cellular immune response, especially a t helper type 1 response, is related to the natural defense against hpv-related cervical lesions, the interleukin 2 response of peripheral blood lymphocytes in vitro to overlapping peptides from hpv-16 e6 and e7 oncoproteins was compared with the degree of cervical cytological abnormality among 140 women in a cross-sectional study. we compared 66 women ... | 1996 | 8752165 |
| the dimeric dna binding domain of the human papillomavirus e2 protein folds through a monomeric intermediate which cannot be native-like. | the dimeric dna binding domain of the human papillomavirus e2 protein displays a two-state concerted unfolding and dissociation, with no detectable monomeric intermediate species accumulated at equilibrium. we investigated the kinetic folding mechanism of the dimeric domain using stopped-flow spectroscopic techniques and observed a fast forming monomeric intermediate, followed by a slower bimolecular reaction. both phases involve secondary structure rearrangements of similar magnitude. our resul ... | 1996 | 8756330 |
| [comparison of human papilloma virus-dna in condyloma acuminatum, cervical cancer and the female genital tract]. | to examine human papilloma virus (hpv) infection rate and types in several lesions of the genital tract and to observe the relation between hpv and host cells for differential handling of cases and for early diagnosis. | 1996 | 8758791 |
| tenascin in human papillomavirus associated lesions of the uterine cervix. | the immunohistochemical expression of tenascin was studied in 80 morphologically diagnosed condylomas and cervical intraepithelial neoplasia (cin) lesions. the results were compared with the human papillomavirus (hpv) dna subtype, which was determined by hpv dot blot and in situ hybridisation. tenascin mrna synthesis was also determined in 10 selected cases by in situ hybridisation. no statistically significant association was found between tenascin expression and the degree of dysplasia or the ... | 1996 | 8763275 |
| [the dosage-performance effects on ca-ski and hpk cells in relation to the dose and fractionation]. | the steep decrease of dose and dose-rate in brachytherapy implies very different radiobiological considerations of the biological effectivity. | 1996 | 8765347 |
| labelling pattern obtained by non-isotopic in situ hybridization as a prognostic factor in hpv-associated lesions. | in the study of infection of the lower female genital tract caused by human papillomavirus (hpv), one of the main concerns is the search for prognostic factors to predict the evolution of premalignant low- and high-grade squamous intraepithelial lesions. this study has evaluated the prognostic usefulness of the patterns of positive reaction obtained by non-isotopic in situ hybridization (nish), referred to as diffuse, punctate, or mixed 'labelling patterns'. the study examined 141 vulvar and ute ... | 1996 | 8774482 |
| growth inhibition of human cervical cancer cells with the recombinant adenovirus p53 in vitro. | human papillomavirus (hpv) has been identified in the majority of invasive cancers of the uterine cervix sampled and has been found to contribute in a significant way to the genesis of human cervical cancer. hpv has two transforming genes that encode the oncoproteins e6 and e7. e6 can form complexes with p53 and promote p53 degradation. we introduced wild-type p53 into a cervical cancer cell line via a recombinant adenoviral vector, ad5cmv-p53. human cervical cancer cell line hela, which has hpv ... | 1996 | 8774641 |
| human papillomavirus in oral premalignant lesions. | the aetiology of oral premalignant lesions is generally accepted to be multifactorial. tobacco and alcohol are established as important cofactors in malignant development in the oral cavity, but in addition microorganisms, such as human papillomavirus (hpv), have gained much interest over the past decade. for many years, hpv has been accepted as an important cofactor in the development of cervical cancer, originating from a mucous membrane with similarities to the oral mucosa. 49 patients with o ... | 1996 | 8776424 |
| the pathology of vulvar neoplasia. | current literature on the pathology of vulvar disease continues to focus on vulvar intraepithelial neoplasia, the role of human papillomavirus in vulvar neoplasia, and paget's disease of the vulva. of particular concern is an increasing incidence of vulvar intraepithelial neoplasia noted over the past several decades, specifically among younger women. human papillomavirus has emerged as a major factor in this trend. furthermore, a more aggressive course may ensue in these young women, requiring ... | 1996 | 8777261 |
| frequent association of human papillomavirus 16 and 18 dna with anal squamous cell and basaloid carcinoma. | human papillomaviruses (hpvs) play a major role in the development of genital neoplasia. their role in anal carcinogenesis is less clear, and the rate of hpv genome detected in invasive anal carcinoma varies considerably in the different reports. moreover, the relationship of hpv to basaloid carcinoma, claimed to represent a histologic type different from the common squamous cell carcinoma, is still controversial. by use of both polymerase chain reaction and southern blot hybridization on dna ex ... | 1996 | 8782197 |
| detection of human papillomavirus type-16 dna utilising microtitre-plate based amplification reactions and a solid-phase enzyme-immunoassay detection system. | the development of a nested polymerase chain reaction (pcr) assay to detect low concentrations of human papillomavirus type-16 (hpv-16) dna for epidemiological studies is described. the pcr utilises primers located in the e5 open reading frame, has an analytical sensitivity of 4 hpv-16 genomes and does not produce amplicons from other common genital hpvs (types-6, -11, -18, -31 and 33). this assay was carried out in 96-well plates utilising internal primers labelled with dinitrophenol (dnp) and ... | 1996 | 8783151 |
| anal carcinoma: a clinical approach to p53 and rb gene proteins. | the role of p53, retinoblastoma (rb) and c-erbb-2 gene proteins in the pathogenesis of anal carcinomas has been examined. a total of 97 patients were included in the study. c-erbb-2, rb and p53 proteins were abnormal in 0, 5 and 34% of the cases, respectively. no correlation was observed between p53 and age, sex, tumour stage, histopathological tumour grading, human papilloma virus (hpv) affection or prognosis. it is concluded that the c-erbb-2 gene is not involved in the development of anal car ... | 1996 | 8784470 |
| a novel genital human papillomavirus (hpv), hpv type 74, found in immunosuppressed patients. | the genome of a novel human papillomavirus (hpv) type, hpv74, was cloned from an iatrogenically immunosuppressed woman with persisting low-grade vaginal intraepithelial neoplasia. hpv74 was found to be phylogenetically related to the low-risk hpv types 6, 11, 44, and 55. hpv74 or a variant of this type was found in specimens from three additional immunosuppressed women but not in about 3,000 anogenital specimens from immunocompetent patients. | 1996 | 8784613 |
| topical 5-fluorouracil in the treatment of cervical human papillomavirus infection. | chemosurgical treatment of vaginal/cervical human papillomavirus (hpv) infection with 5-fluorouracil (5-fu) is limited by vulvar pain and dysuria resultant from leakage and vulvar/urethral tissue reaction to the 5-fu. women with cervical hpv dna identified by in situ hybridization on colposcopically directed biopsy were treated with topically applied 5% 5-fu cream. a fitted cervical cap was used to restrict exposure to 5-fu. repeat biopsies and in situ hybridization at least 6 weeks after applic ... | 1996 | 8793499 |
| natural variants of the human papillomavirus type 16 e6 protein differ in their abilities to alter keratinocyte differentiation and to induce p53 degradation. | three naturally occurring variant human papillomavirus type 16 (hpv-16) e6 proteins, which contained amino acid substitutions predominantly near the n terminus, exhibited significant differences in their abilities to abrogate keratinocyte differentiation in response to serum and calcium and to induce the degradation of p53 in vitro. one variant surpassed the reference e6 protein in its ability to abrogate keratinocyte differentiation responses, whereas another showed a reduction in this activity ... | 1996 | 8794343 |
| detection of human papillomavirus in cervical biopsies of summer camp rotc cadets with abnormal papanicolaou smears. | human papillomavirus (hpv) infections may be the most common sexually transmitted disease (std) among adolescents and young adults. a strong association exists for certain "high-risk" hpv-type cervical infections and subsequent evolution of cervical cancer and its precursor lesions. the objective of this study was to detect hpv in cervical biopsies of reserve officer training corps (rotc) cadets attending a regional summer camp who had abnormal screening papanicolaou (pap) smears and to better d ... | 1996 | 8795786 |
| ethnic differences in risk factors associated with genital human papillomavirus infections. | this study was a cross-sectional analysis of different risk and cofactors for genital human papillomavirus (hpv) infection among 302 women in four ethnic groups at 10 clinic sites. ethnicity, rather than hpv status, accounted for most of the differences among the groups on five risk factors: age at first sex, number of lifetime sexual partners, number of partners' sex partners, number of 'once only' sex partners, and number of 'long-term' sexual relationships. there were also ethnic differences ... | 1996 | 8796472 |
| the psychosocial impact of human papillomavirus infection: implications for health care providers. | the american social health association (asha) surveyed people with human papillomavirus (hpv) about their experiences with the disease and its effect on their lives. a sample of 837 was chosen from the subscribers to hpv news, asha's quarterly journal for people with hpv. of the sample, 489 returned completed surveys, which addressed medical history, health care experiences, personal impact, and demographic information. data analysis was descriptive. data illustrated that the psychosocial impact ... | 1996 | 8799782 |
| the e6 variant proteins e6i-e6iv of human papillomavirus 16: expression in cell free systems and bacteria and study of their interaction with p53. | several species of alternatively spliced mrnas are transcribed from the e6 gene region of human papillomavirus (hpv) 16. these have the coding capacity for either the full length e6 of 151 amino acids (aa) or four truncated variants, e6i-e6iv, of 43-64 aa. as the first step to identify the putative e6 variants and their functions, we generated cdnas corresponding to the various e6 open reading frames (orf) and examined their expression employing in vitro transcription/translation systems and the ... | 1996 | 8806176 |
| human papillomavirus type 16 e6 protein up-regulates the expression of the high mobility group protein hmg-i(y) gene in mouse 10t1/2 cells. | using a differential hybridization technique, we have identified a mouse cellular gene, high mobility group protein hmg-i(y), whose expression is up-regulated by the e6 protein of human papillomavirus (hpv) type 16. this gene was overexpressed in e6-expressing mouse 10t1/2 cells, but not in g418-resistant 10t1/2 cells. the expression of the hmg-i(y) gene was up-regulated by the transient expression of e6 from a zinc-inducible human metallothionein-iia gene promoter. expression was found to be mo ... | 1996 | 8806179 |
| hpv6 variants from malignant tumors with sequence alterations in the regulatory region do not reveal differences in the activities of the oncogene promoters but do contain amino acid exchanges in the e6 and e7 proteins. | human papillomavirus type 6 (hpv6) causes benign epithelial proliferations of the anogenital and aerodigestive tract, which usually tend to regress spontaneously. the low incidence of hpv6 in carcinomas and the rare progression of the benign tumors has led to the classification of hpv6 as "low-risk" virus. a series of reports, however, described the isolation of hpv6 variants from malignant tumors characterized by sequence rearrangements in the noncoding regulatory region (ncr). it was speculate ... | 1996 | 8806552 |
| the e5 gene of hpv-16 enhances keratinocyte immortalization by full-length dna. | human papillomavirus type 16 (hpv-16) is the most common papillomavirus genome found in human cervical cancers and its dna is capable of immortalizing primary keratinocytes in vitro. when expressed by their native promoter, two separate hpv-16 oncogenes, e6 and e7, cooperate to immortalize primary human keratinocytes. early hpv cervical lesions express abundant amounts of e5-specific rna, and using a quantitative keratinocyte immortalization assay, we demonstrate here that e5 can act in cis to i ... | 1996 | 8806560 |
| p53-independent growth regulation of cervical cancer cells by the papillomavirus e6 oncogene. | growth of cervical carcinoma cells depends on continuous expression of high risk type human papillomavirus oncogenes e6 and e7. e6 destabilizes p53, a tumor-suppressive transcription factor, which activates expression of the inhibitor of cell cycle progression p21 and other genes. e6-mediated p53 degradation can therefore result in cell cycle deregulation. it has, however, not yet been determined whether p53 inactivation is sufficient to provoke cell cycle progression in established cervical car ... | 1996 | 8806692 |
| serological responses to human papillomavirus type 6 and 16 virus-like particles in patients with cervical neoplastic lesions. | serum samples from 36 cervical carcinoma patients, 33 patients with high-grade squamous intraepithelial lesions, and 31 cytologically normal women were tested by enzyme-linked immunosorbent assay (elisa) using human papilloma virus type 6 (hpv 6) and hpv 16 virus-like particles as antigens. forty serum specimens from 1-year-old children were used to assign cutoff points. when serum samples from the subjects infected with hpv 16 were tested in an hpv 16 elisa detecting immunoglobulin a (iga), igg ... | 1996 | 8807204 |
| activation of local cell-mediated immunity in interferon-responsive patients with human papillomavirus-associated lesions. | successful immune response to viral infection, such as human papillomavirus (hpv) infection, involves presentation of viral antigens to the immune system, recruitment of t cells and macrophages, and activation of a diverse array of cytokines. interferons (ifn) are known to exert immunomodulatory functions via activating these pathways. however, the presence of hpv can interact with this process. we employed a reverse transcription-polymerase chain reaction (rt-pcr)-based method to study this phe ... | 1996 | 8807499 |
| transcriptional control of human papillomavirus type 18 oncogene expression in different cell lines: role of transcription factor yy1. | binding of yy1 to the proximal fragment of the human papillomavirus type 18 (hpv-18) upstream regulatory region (urr) activates the oncogene expression of hpv-18 in hela cells, whereas in hepg2 cells this expression is repressed by yy1. in the present transient transfection study, we analyze the regulation of the hpv-18 urr by yy1 in an extended number of cell lines. except for hela cells, yy1 represses or does not influence oncogene expression in all cell lines tested. in hela cells the activat ... | 1995 | 8808335 |
| [p53 protein and human papillomavirus oncogenes in carcinogenesis of the uterine cervix]. | human papillomavirus (hpv) dna sequences are found in the majority (90%) of cervical tumors, and in cell lines derived from them. the products of the viral e6 and e7 oncogenes inactivate those from the p53 and rb tumor suppressor genes. the p53 protein controls the entrance to the cell cycle. when dna damage occurs, p53 levels are increased, resulting in cell arrest. this allows cells to repair the damage before replication occurs. cells without p53 (either mutated or absent) will replicate thei ... | 1996 | 8815489 |
| genomic instability in squamous cell carcinoma of the head and neck. | the role of genomic instability in the development of squamous cell carcinoma (scchn) has become apparent with the publication of three major allelotype analysis of this disease, as well as many publications which have concentrated on specific target regions. the measurement of accumulated genetic alterations or fractional allele loss, as determined by allelotype analysis, provides a useful molecular indicator of tumour behaviour. in one major study, a positive correlation was found between fal ... | 1996 | 8816845 |
| temporal and histologic relationships of proliferating cell nuclear antigen and human papillomavirus type 11 in the mouse xenograft system. | proliferating cell nuclear antigen (pcna) is an accessory protein of dna polymerase delta. this protein is associated with cell cycle progression and can be detected in the replicating cells of normal tissues. condylomata acuminata are benign epithelial tumors caused by infection with human papillomaviruses and are characterized by abnormal cell proliferation. the athymic mouse xenograft model of hpv 11 infection was used to test the hypothesis that pcna is induced early in the course of hpv 11 ... | 1996 | 8825719 |
| the relationship of human papillomavirus-related cervical tumors to cigarette smoking, oral contraceptive use, and prior herpes simplex virus type 2 infection. | it has now been established that infection with human papillomavirus (hpv) is necessary for the development of most cervical cancers. hpv is not sufficient for the development of cancer. other exposures or host factors are necessary for cancer to occur. as part of an ongoing, population-based case-control study of invasive cervical cancer, we investigated the role of cigarette smoking, oral contraceptive (oc) use, and herpes simplex virus type 2 (hsv-2) as potential cofactors with hpv in the dev ... | 1996 | 8827359 |
| human papillomavirus and cancers of the upper aerodigestive tract: a review of epidemiological and experimental evidence. | human papillomaviruses (hpvs) are recognized as important causes of cancer of the anogenital tract and may be involved also in the etiology of cancers of the upper aerodigestive tract (uadt). epidemiological and experimental evidence lend some support to this possibility. increased risk of cancer of the oral cavity, pharynx, and larynx subsequent to the occurrence of cancer of the cervix has been found and suggests common etiological factors besides smoking. hpv has been found in a substantial p ... | 1996 | 8827362 |
| diagnostic performance of hybrid capture human papillomavirus deoxyribonucleic acid assay combined with liquid-based cytologic study. | our purpose was to determine the diagnostic performance of human papillomavirus deoxyribonucleic acid testing when performed on liquid-based (thin-prep) cytologic samples. | 1996 | 8828429 |
| oxytocin-stimulated responses in a pregnant human immortalized myometrial cell line. | smooth muscle cells isolated from the myometrium of a pregnant woman at term were infected with a replication-defective adenovirus vector expressing the e6/e7 proteins of human papilloma virus 16. a clonal line, phm1-41, was selected by resistance to geneticin and examined for maintenance of smooth muscle phenotype and response to oxytocin. the immortalized cell line retained morphological characteristics of proliferating smooth muscle cells in culture for up to 22 passages and has been used for ... | 1996 | 8828850 |
| bacterial expression and immunological detection of human papillomavirus type 16 e7 protein. | we have expressed an unfused e7 protein from human papillomavirus 16 into escherichia coli by using a t7-rna polymerase system. e7 mrna was detected one hour after promoter induction. western blot analysis using either a murine monoclonal antibody elicited against e7 or sera from cervical carcinoma patients demonstrated that recombinant e7 expressed in e. coli reacted to both of them, and a 21 kd band is observed as a positive signal. this protein provides a suitable material for further protein ... | 1996 | 8831707 |
| detection of human papillomavirus in routinely processed biopsy specimens from laryngeal papillomas: evaluation of reproducibility of polymerase chain reaction and dna in situ hybridization procedures. | the frequency of human papillomavirus (hpv) in laryngeal papillomas varies largely among different studies. dna in situ hybridization (ish) has been the most widely used method for detection of hpv. the aim of this study was to compare the reproducibility and sensitivity of ish with polymerase chain reaction (pcr) in 35 specimens of laryngeal papillomas routinely fixed in buffered or unbuffered formalin. out of 12 specimens fixed in buffered formalin, 10 were positive for hpv 6/11 using ish. the ... | 1996 | 8831853 |
| studies on in vivo induction of cytotoxic t lymphocyte responses by synthetic peptides from e6 and e7 oncoproteins of human papillomavirus type 16. | induction of cytotoxic t lymphocyte (ctl) responses is an important defense mechanism against infectious agents, specifically viruses. in the present investigation we employed a mouse assay system we previously developed, for rapid induction of ctls by synthetic peptides from e6 and e7 oncoproteins of human papillomavirus type 16 (hpv-16). in particular, we compared the efficiency of ctl induction by hpv-16 peptides synthesized as linear monomers with those containing a dipalmitoyl-lysine-glycin ... | 1995 | 8833270 |
| histopathologic examination of penile epithelial lesions is of limited diagnostic value in human papillomavirus infection. | guidelines for the histopathologic diagnosis of human papillomavirus (hpv) infection have been drawn from changes seen in cervical specimens not necessarily applicable to penile epithelium. | 1996 | 8836023 |
| staurosporine resistance accompanies dna tumor virus-induced immortalization and is independent of the expression and activities of erk1, erk2, cyclin a, cyclin-dependent kinase (cdk) 2, and cdk4. | staurosporine, a potent protein kinase inhibitor, has been shown to arrest the growth of a number of normal cell types in the g1 phase of the cell cycle, while having little effect on several transformed lines. we wished to determine whether increased resistance to staurosporine was a common feature of virus-immortalized human cells and whether this phenotype was an early event following the expression of sv40 tumor antigens. human foreskin keratinocytes immortalized by the sv40 dna tumor virus ... | 1996 | 8838866 |
| is the evidence implicating human papillomavirus type 16 in esophageal cancer hard to swallow? | 1996 | 8841013 | |
| human papillomavirus-associated cervical intraepithelial neoplasia following lesbian sex. | less than 3% of lesbians develop cervical dysplasia, with increasing risk correlating with previous heterosexual activity. because they are not currently sexually active with men, many lesbians do not perceive themselves to be at risk for developing dysplasia and do not obtain regular papanicolaou smears. there are no standard recommendations for papanicolaou smear intervals for lesbians. | 1996 | 8841259 |
| human papillomavirus expression in oral mucosa, premalignant conditions, and squamous cell carcinoma: a retrospective review of the literature. | the literature of human papillomavirus detection in normal oral mucosa and oral lesions associated with the dysplastic progression of epithelium was reviewed to help define the role of this virus in the development of oral squamous cell carcinoma. | 1996 | 8843455 |
| medical therapy of genital human papilloma virus-related disease. | 1995 | 8845396 | |
| the atp-binding and atpase activities of human papillomavirus type 16 e1 are significantly weakened by the absence of prolines in its atp-binding domain. | the e1 protein of human papillomavirus (hpv) type 16 is the only known papillomavirus e1 which does not contain any proline residues in the phosphate-loop (p-loop) of its atp-binding site. to ascertain whether this feature influences the activities of hpv-16 e1, we generated a mutant hpv-16 e1 (e1pro) in which prolines are inserted in place of alanines in this site, making the p-loop identical to its bovine papillomavirus type 1 counterpart. glutathione s-transferase (gst) fusion proteins (gste1 ... | 1995 | 8847499 |
| sexually transmitted diseases in australia: a decade of change. epidemiology and surveillance. | survival data in the last decade for sexually transmitted diseases (stds) other than human immunodeficiency virus (hiv) are uneven across the states/territories. the incidence of gonorrhoea decreased by more than 80%, but at different times in different patient groups, different states, and different anatomical sites. there was a resurgence of rectal gonorrhoea in homosexual men in 1989-1991. resistance to penicillin steadily increased, and partial resistance to quinolones has emerged. there was ... | 1995 | 8849191 |
| genital oncogenic human papillomavirus infection: a short review on the mode of transmission. | the most common oncogenic human papillomaviruses (hpvs) detected in the female lower genital tract are hpv types 16 and 18. they are ancient viruses which have evolved with mankind over the last 200 000 years. the identification of their roles in the development of carcinoma of the uterine cervix in the last decade fills the gap between the long-sought link between sexual behaviour and risk of cervical cancer. indeed, epidemiological, clinical and sophisticated molecular studies employing the po ... | 1995 | 8849195 |
| human papillomavirus and epstein-barr virus in sinonasal schneiderian papillomas. an in situ hybridization and polymerase chain reaction study. | using the polymerase chain reaction (pcr), it has been recently reported that the epstein-barr virus (ebv) is present in the majority of schneiderian sinonasal papillomas (snp) of the inverted type and may play a role in the pathogenesis of these lesions. the reported prevalence rates of human papillomavirus (hpv) in different types of snp is also controversial and in need of clarification. twenty-eight snp from 27 patients were histologically classified and evaluated for evidence of ebv using p ... | 1996 | 8853035 |
| recurrent respiratory papillomatosis with malignant transformation in a young adult. | the term 'papilloma' was first used by mackenzie 100 years ago, who claimed that this was the most benign tumour of the larynx. today papillomas are considered to be caused by the human papilloma virus group (h.p.v.). the majority of patients suffering from this disease which is also referred to as 'recurrent respiratory papillomatosis' require multiple surgical operations for tumour removal. malignant transformation of papillomas, which is a rare condition, is considered to occur mainly to irra ... | 1996 | 8857327 |
| hiv infection in women: an escalating health concern. | the proportion of women infected with the human immunodeficiency virus (hiv) continues to increase. over one-half of women acquire the virus through heterosexual contact. the diagnoses that define the acquired immunodeficiency syndrome and the use of antiretroviral therapy are similar in men and women, except in pregnancy. however, management decisions differ significantly regarding contraceptive and gynecologic care. besides abstinence, use of the latex condom continues to be the most effective ... | 1996 | 8857778 |
| demonstration of human papillomavirus type 16-related dna and absence of detectable p53 gene mutations in widespread cutaneous squamous cell carcinomas after oral psoralen with uv-a treatment. | 1996 | 8859052 | |
| use of multiple pcr primer sets for optimal detection of human papillomavirus. | using multiple pcr primer sets, we tried to optimize the detection of human papillomavirus (hpv) in dna samples isolated from 361 frozen biopsy specimens from patients with invasive cervical carcinomas. the hpvs detected were placed into three distinct groups, including group i/inex at telelab (skien, norway) and group ineg and group ii at the norwegian radium hospital (oslo, norway). the consensus primer sets were oli-1b-oli-2i, my09-my11, gp5-gp6, and gp(5+)-gp6+ from the hpv l1 gene and cpi-c ... | 1996 | 8862564 |
| response to interferon treatment decreases with epidermal dedifferentiation in condylomas. | after interferon (ifn) treatment of patients with condyloma acuminatum, groups clinically proven to be responders or nonresponders were selected, and cellular parameters that might influence the clinical response were studied in pretreatment biopsies by reverse transcription polymerase chain reaction (rt-pcr). the nonresponders were found to express higher amounts of cellular proliferative markers, such as proliferating cell nuclear antigen (pcna), cyclin a, and cdc 2 kinase, but lower levels of ... | 1996 | 8863992 |
| loss of seroreactivity against human papillomavirus (hpv) in bone marrow transplant recipients. | sera from 19 autologous and 35 allogeneic bone marrow transplant (bmt) patients at huddinge university hospital were analyzed by different elisa assays before and 1 year after bmt for the presence of igg antibodies towards human papillomavirus (hpv). one assay was a peptide-based enzyme-linked immunoadsorbent assay (elisa). these peptides were derived from the amino acid sequences of the two major viral capsid proteins of hpv 16, p(31) l1 and (p49) l2. the other was an elisa using hpv-type 16 vi ... | 1996 | 8864443 |
| nonradioisotopic detection and typing of human papillomaviruses by use of polymerase chain reaction and single-strand conformation polymorphism. | the polymerase chain reaction (pcr), used to detect human papillomavirus (hpv), is finding increasing applications in clinical laboratories. the standard method of analysis to detect amplified pcr products is ethidium bromide gel electrophoresis combined with labor intensive blot hybridization. in this study, we describe single-strand conformation polymorphism (sscp) to detect and genotype simultaneously general primer gp5+/gp6+ amplified hpv dna using semiautomated electrophoresis on polyacryla ... | 1996 | 8866235 |
| absence of human papillomavirus dna in breast cancer as revealed by polymerase chain reaction. | oncogenic human papillomavirus (hpv) types 16 and 18 commonly associated with cervical cancer are found in many epithelial malignancies at extra-genital sites including breast. the transforming gene products of hpv have also been shown to immortalize breast epithelial cells in vitro. but the findings of hpv dna in breast carcinoma are found to be contradictory. in the present study fine needle aspirate cell (fnac) samples from 26 breast cancer patients and four breast tumour biopsies were analys ... | 1996 | 8872328 |
| oncological problems in aids--a review of the clinical features and management. | since the beginning of the acquired immunodeficiency syndrome (aids) epidemic in the early 1980s, several malignant conditions have been recognised to be associated with this syndrome which affect up to 40% of aids patients at some stage of their illness. they include kaposi's sarcoma, systemic non-hodgkin's lymphoma, primary central nervous system lymphoma and invasive cervical cancer. kaposi's sarcoma and primary central nervous system lymphoma were tumours rarely seen below the age of 50 prio ... | 1996 | 8876905 |
| e7 proteins of four groups of human papillomaviruses, irrespective of their tissue tropism or cancer association, possess the ability to transactivate transcriptional promoters e2f site dependently. | in an experimental system in which an expression vector including the e7 gene of a given human papillomavirus (hpv), together with a luciferase reporter plasmid including the adenovirus e2 (ad e2) promoter, was transiently transfected into cultured mouse nih3t3 fibroblastic cells, we obtained the signal indicating that e7 proteins of hpv type 5, 12, 14, 20, 21, 25, and 47, which are associated with epidermodysplasia verruciformis (ev), can transactivate the ad e2 promoter, as previously reported ... | 1996 | 8879118 |
| risk factors associated with genital hpv infection. | the genital human papillomavirus (hpv) is directly associated with cervical cancer, the second most common form of cancer among women. the study was guided by a synergistic interaction model of hpv risk factors. the relative risk of selected risk factors and cofactors associated with genital hpv infections was identified. women at highest risk for acquiring an hpv infection had (a) initiated sex before age 15, (b) more than four lifetime sex partners, (c) more than one "once only" sexual partner ... | 1996 | 8885483 |
| natural history of cervical neoplasia: overview and update. | current cervical cancer control strategies and specifically, the screening and treatment policies that have been used during the past 30 years, are based on an understanding of the natural history of cervical neoplasia that does not take into account the important role of human papillomavirus in cervical cancer pathogenesis. this survey presents a review of new data on the causes of cervical cancer and the role of human papillomavirus and recommends the integration of these data. incorporation o ... | 1996 | 8885793 |
| clinicopathologic analysis of 370 cases of vulvar intraepithelial neoplasia. italian study group on vulvar disease. | to investigate epidemiologic, clinical and pathologic aspects of various grades of vulvar intraepithelial neoplasia (vin). | 1996 | 8887191 |
| human papillomavirus type 11 and 16 e5 represses p21(wafi/sdii/cipi) gene expression in fibroblasts and keratinocytes. | here we report that the e5 proteins of human papillomavirus type 11 (hpv-11) and hpv-16 suppressed the expression of the p21(wafi/sdii/cipi) tumor suppressor gene in nih 3t3 cells and immortalized human keratinocytes. the promoter activity of p21 was repressed by e5 of hpv-11 and -16, suggesting that p21 gene suppression by e5 was at the transcriptional level. using an inducible system, we demonstrated that increased induction of hpv-11 e5 in nih 3t3 cells and keratinocytes led to increased repr ... | 1996 | 8892872 |
| a novel c/ebp beta-yy1 complex controls the cell-type-specific activity of the human papillomavirus type 18 upstream regulatory region. | the human papillomavirus type 18 (hpv-18) upstream regulatory region (urr) controls viral gene transcription in a cell-type-specific manner. the hpv-18 urr is active in hela cells but inactive in hepg2 cells. the activating activity of yy1 in hela cells is dependent on its functional interactions with the switch region which is critical for the hpv-18 urr activity in hela cells. here, we show that a protein complex composed of c/ebp beta and yy1 binds the switch region which is detected only in ... | 1996 | 8892890 |
| epidemiologic determinants of seroreactivity to human papillomavirus (hpv) type 16 virus-like particles in cervical hpv-16 dna-positive and-negative women. | the epidemiologic determinants of seroreactivity to human papillomavirus (hpv) type 16 l1/l2 virus-like particles (vlps) were assessed separately in hpv-16 dna-positive and -negative women participating in a nested case-control study of incident cervical neoplasia. seventy-four women with cervical hpv-16 dna and 656 cytologically normal hpv-16 dna-negative subjects were interviewed and tested at two time points for viral dna and once (at the later time) for vlp seroreactivity. among subjects who ... | 1996 | 8896493 |
| comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus dna by pcr in paraffin-embedded cervical carcinomas. | we have compared the efficacies of three general primer pairs for the detection of human papillomavirus (hpv) dna in formaldehyde-fixed paraffin-embedded carcinomas. the use of these primer pairs leads to underestimates of the hpv prevalence (gp5/6, 61.1%; cpi/iig, 57.4%; my09/11, 46.9%; combined, 72.8%). the efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. by using newly developed type-specific primer pairs (amplimer length, approximately 10 ... | 1996 | 8904451 |
| type of human papillomavirus is related to clinical features of cervical carcinoma. | despite the fact that approximately ten types of human papillomavirus (hpv) are associated with cervical carcinoma, the question of whether the hpv type present in cervical carcinoma is related to clinical behavior had yet to be answered when this study was conducted. | 1996 | 8909314 |
| the adeno-associated virus rep78 major regulatory/transformation suppressor protein binds cellular sp1 in vitro and evidence of a biological effect. | adeno-associated virus (aav) rep78 is a multifunctional protein that is required for aav transcriptional activity, aav dna replication, and possibly for site-specific integration of aav into human chromosome 19. rep78 is also able to inhibit a variety of heterologous promoters, including those of c-h-ras, human papillomavirus types 16 and 18, and hiv type 1. however, rep78 is unable to significantly affect murine osteosarcomavirus (msv). it was noticed that promoters that are inhibited possess b ... | 1996 | 8912872 |
| induction of an antibody response in mice against human papillomavirus (hpv) type 16 after immunization with hpv recombinant salmonella strains. | human papillomaviruses (hpv) are present in approximately 95% of all cervical carcinomas and the hpv e6 and e7 genes are continuously expressed in these lesions. there is also circumstantial evidence that often natural immunity against hpv is generated and that this is of influence on hpv-induced lesions. stimulation of the immune system by proper presentation of relevant hpv antigens might, therefore, lead to a prophylactic or therapeutic immunological intervention for hpv-induced lesions. for ... | 1996 | 8917635 |
| detection of type specific human papillomavirus (hpv) dna in cervical cancers of indian women. | commercial vira pap and vira type kits of life technologies inc., u.s.a., were used to determine prevalence and type specific distribution of human papilloma viruses (hpv) in 18 biopsy samples of cervical carcinomas and 26 specimens of exfoliated cervical cells (swabs). the women were either attending out-patient's department of a suburban hospital or a cancer hospital in calcutta, india. hpv dna was detected in 9 biopsy specimens but not in any of the cervical swabs. five of the 9 hpv positive ... | 1995 | 8919467 |
| papillomaviruses and potential copathogens. | an in vitro multistage genital epithelial cell model for cervical cancer that parallels the in vivo neoplastic process has been developed using recombinant human papillomavirus (hpv) dna and genital cells. hpv-16-immortalized genital cells are responsive to the genotoxic action of known chemical carcinogens (polycyclic hydrocarbons, alkylating agents or cigarette smoke condensate), but are not converted to malignancy. ras oncogene and human herpes virus-2 did convert hpv immortalized cells to ma ... | 1996 | 8920709 |
| up-regulation of hormone response of human papillomavirus type 16 expression and increased dna-protein binding by consensus mutations of viral glucocorticoid response elements. | human papillomaviruses (hpvs) and steroid hormones are linked to the development of cervical cancer. studies from our laboratory and others showed that the steroid glucocorticoid and progesterone hormones activated the expression of hpv type 16. this activation was attributed to the specific interaction of the glucocorticoid receptor (gr) with the three glucocorticoid response elements (gres) in the hpv16 regulatory region. in the present study, we first examined the glucocorticoid response medi ... | 1996 | 8923291 |
| [retrospective study of cervical papillomavirus lesions: early herpes simplex virus proteins as markers of risk for progression]. | specific types of human papillomavirus (hpv) are currently implicated as etiologic agent of benign and malignant lesions of the genital tract. previous studies highlighted the presence of early proteins (icsp 11/12) of herpes simplex virus type 2 (hsv-2) mainly in hpv infected lesions of the cervix. this paper reports a retrospective study on the clinical course of benign cervical alterations in a 70 women population followed-up for 24 months. hpv and hsv-2 (late and early) antigens were detecte ... | 1996 | 8927276 |
| [maternal-fetal transmission of human papillomavirus]. | human papilloma virus (hpv) infection is one of the most frequently observed sexually-transmitted diseases (10-60% of the general population). in pregnant women, as well as accelerating the evolution of dysplasia to cervical cancer, the infection may be transmitted to the fetus during gestation or at the time of birth. children who have been infected at birth may develop laryngeal papillomatosis during the first 5 years of life that may, in some cases, spread to the point of causing aphonia or s ... | 1996 | 8927279 |
| p53 protein expression in esophageal squamous cell papillomas: a study of 36 lesions. | in the present study 36 esophageal squamous cell papillomas (escp), uncommon benign neoplasms of the esophagus, were investigated retrospectively for p53 immunoreactivity by using the monoclonal antibody pab 1801. two escps had previously been found to be human papillomavirus (hpv) type-6-positive. | 1996 | 8927934 |
| cytokine regulation of the matrix metalloproteinases and their inhibitors in human papillomavirus-18 transformed human prostatic tumor cell lines. | cytokines may play a critical role in influencing the invasive and metastatic behavior of advanced cancers. to investigate the influence of cytokines on tissue inhibitor of metalloproteinase (timp) and matrix metalloproteinase (mmp) expression we have established cultures from prostate tissues of low gleason sum 5 and high gleason sum 10 cancers. we have examined the influence of different cytokines (interleukin [il]-10, il-4, il-6, il-2, and interferon-gamma) on timp-2, mmp-2, and mmp-9 protein ... | 1996 | 8938794 |
| induction of the hpv16 enhancer activity by jun-b and c-fos through cooperation of the promoter-proximal ap-1 site and the epithelial cell type--specific regulatory element in fibroblasts. | the epithelial cell type-specific enhancer of the human papillomavirus (hpv) type 16 termed the long control region (lcr) carries three ap-1 binding sites. we investigated the roles of the ap-1 sites for transactivation of the lcr by jun-b that may be a cell type specific-transactivator for the hpvs in human fibroblasts in which expression of the endogenous jun-b gene is low. transient expression of jun-b alone poorly activated transcriptional activity of the lcr. however, when combined with c-f ... | 1996 | 8938978 |
| [local immune responses in uterine cervical carcinogenesis]. | in order to investigate the role of local immune response in uterine cervical carcinogenesis, lymphocyte phenotypes infiltrating the cervical region were studied by indirect immunoperoxidase staining for natural killer (nk) cells, macrophages. langerhans cells (lc), memory t cells, cd4-positive cells and cd8-positive cells. the specimens used in this study were 9 normal ectocervical epithelium samples, 28 with mild dysplasia, 28 with moderate dysplasia, 31 with severe dysplasia and 9 with carcin ... | 1996 | 8940695 |
| human papillomavirus dna in squamous cell carcinoma of the lung. | to compare the incidence of squamous cell carcinoma (scc) of the lung in okinawa with that in niigata on the mainland. | 1996 | 8943746 |
| different hla-dr-dq haplotypes are associated with cervical intraepithelial neoplasia among human papillomavirus type-16 seropositive and seronegative swedish women. | to analyze whether hla may be a determinant of the risk of developing cervical cancer precursor lesions, the association between hla and cervical neoplasia among hpv16-seropositive and -negative subjects was determined in a population-based cohort in the västerbotten county of northern sweden. hla genotyping of dr and dq was done by pcr in 74 patients and 164 healthy controls matched for age, sex and area of residence. the presence of dqa1*0102 was weakly associated with cervical neoplasia in hp ... | 1996 | 8945607 |
| risk factors for hpv dna detection in middle-aged women. | strong epidemiologic evidence indicates that human papillomavirus (hpv) is the main etiologic factor of cervical cancer. a few cohort studies suggest that most hpv infections are transient in young women and that persistent hpv infections are more common in older women. little is known about the determinants of persistent hpv infections. the present study was aimed at increasing our knowledge about these determinants. | 1996 | 8946637 |