Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| eliciting renal failure in mosquitoes with a small-molecule inhibitor of inward-rectifying potassium channels. | mosquito-borne diseases such as malaria and dengue fever take a large toll on global health. the primary chemical agents used for controlling mosquitoes are insecticides that target the nervous system. however, the emergence of resistance in mosquito populations is reducing the efficacy of available insecticides. the development of new insecticides is therefore urgent. here we show that vu573, a small-molecule inhibitor of mammalian inward-rectifying potassium (kir) channels, inhibits a kir chan ... | 2013 | 23734226 |
| will people change their vector-control practices in the presence of an imperfect dengue vaccine? | human behaviours, which are influenced by social, cultural, economic and political factors, can increase or decrease the risk of dengue infection, depending on the relationship with the insect vector. because no vaccine is currently available, the spread of dengue can only be curtailed by controlling vector populations (aedes aegypti and others) and by protecting individuals. this study tested the hypothesis that dengue-affected populations are likely to relax their vector-control habits if a po ... | 2014 | 23735007 |
| genetic and phenotypic characterization of manufacturing seeds for a tetravalent dengue vaccine (denvax). | we have developed a manufacturing strategy that can improve the safety and genetic stability of recombinant live-attenuated chimeric dengue vaccine (denvax) viruses. these viruses, containing the pre-membrane (prm) and envelope (e) genes of dengue serotypes 1-4 in the replicative background of the attenuated dengue-2 pdk-53 vaccine virus candidate, were manufactured under cgmp. | 2013 | 23738026 |
| identification of dengue viruses in naturally infected aedes aegypti females captured with biogents (bg)-sentinel traps in manaus, amazonas, brazil. | in manaus, the first autochthonous cases of dengue fever were registered in 1998. since then, dengue cases were diagnosed by the isolation of viruses 1, 2, 3, and 4. | 2013 | 23740064 |
| aedes aegypti saliva contains a prominent 34-kda protein that strongly enhances dengue virus replication in human keratinocytes. | 2014 | 23752041 | |
| the influence of dengue virus serotype-2 infection on aedes aegypti (diptera: culicidae) motivation and avidity to blood feed. | dengue virus (denv) is transmitted by aedes aegypti, a species that lives in close association with human dwellings. the behavior of denv-infected mosquitoes needs further investigation, especially regarding the potential influence of denv on mosquito biting motivation and avidity. | 2013 | 23755202 |
| overlapping genes of aedes aegypti: evolutionary implications from comparison with orthologs of anopheles gambiae and other insects. | although gene overlapping is a common feature of prokaryote and mitochondria genomes, such genes have also been identified in many eukaryotes. the overlapping genes in eukaryotes are extensively rearranged even between closely related species. in this study, we investigated retention and rearrangement of positionally overlapping genes between the mosquitoes aedes aegypti (dengue virus vector) and anopheles gambiae (malaria vector). the overlapping gene pairs of a. aegypti were further compared w ... | 2013 | 23777277 |
| epidemiological significance of the breeding of mosquitoes in discarded automobile tyres in zaria, northern nigeria. | waste automobile tyres are suitable receptacles of rainwater thus capable of supporting the breeding of vector mosquito species in close proximity to humans and altering disease transmission risks. bionomics of mosquitoes in carelessly discarded tyres was explored to unravel species composition and abundance, physicochemical parameters of microhabitats and epidemiological significance. of the 1179 larvae isolated from 90/212 (42.45%) positive tyres were ten species belonging to aedes and culex g ... | 2011 | 23781631 |
| modelling interventions during a dengue outbreak. | we present a stochastic dynamical model for the transmission of dengue that considers the co-evolution of the spatial dynamics of the vectors (aedes aegypti) and hosts (human population), allowing the simulation of control strategies adapted to the actual evolution of an epidemic outbreak. we observed that imposing restrictions on the movement of infected humans is not a highly effective strategy. in contrast, isolating infected individuals with high levels of compliance by the human population ... | 2014 | 23800514 |
| aedes (stegomyia) aegypti in the continental united states: a vector at the cool margin of its geographic range. | after more than a half century without recognized local dengue outbreaks in the continental united states, there were recent outbreaks of autochthonous dengue in the southern parts of texas (2004-2005) and florida (2009-2011). this dengue reemergence has provoked interest in the extent of the future threat posed by the yellow fever mosquito, aedes (stegomyia) aegypti (l.), the primary vector of dengue and yellow fever viruses in urban settings, to human health in the continental united states. a ... | 2013 | 23802440 |
| novel estimates of aedes aegypti (diptera: culicidae) population size and adult survival based on wolbachia releases. | the size of aedes aegypti (l.) mosquito populations and adult survival rates have proven difficult to estimate because of a lack of consistent quantitative measures to equate sampling methods, such as adult trapping, to actual population size. however, such estimates are critical for devising control methods and for modeling the transmission of dengue and other infectious agents carried by this species. here we take advantage of recent releases of wolbachia-infected ae. aegypti coupled with the ... | 2013 | 23802459 |
| [problems of medical parasitology]. | situation on parasitic disease in russia remains complex. reduction of parasitology personnel had a negative impact on the quality of epidemiological control in the field of parasitic diseases and resulted in a decrease of awareness of physicians of therapeutic-prophylaxis institutions. the situation was aggravated by a lack of anti-malaria preparations and insufficient specter of anti-helminthic drugs. uncontrolled increase of the number of domestic and stray dogs in cities was the reason for i ... | 2013 | 23805663 |
| genetic control of aedes mosquitoes. | aedes mosquitoes include important vector species such as aedes aegypti, the major vector of dengue. genetic control methods are being developed for several of these species, stimulated by an urgent need owing to the poor effectiveness of current methods combined with an increase in chemical pesticide resistance. in this review we discuss the various genetic strategies that have been proposed, their present status, and future prospects. we focus particularly on those methods that are already bei ... | 0 | 23816508 |
| dengue infections. | dengue fever is one of the most common mosquito-borne viral illnesses in the world. it is usually transmitted to humans through the bite of an infected aedes aegypti or aedes albopictus mosquito. dengue infections are caused by four antigenically distinct but closely related viruses (den 1?4). infection with any one of the viruses is thought to provide lifetime immunity to future infections from the same virus but only short-term cross-immunity to the other types, leading to the possibility of s ... | 2013 | 23817881 |
| molecular evidence for dual pyrethroid-receptor sites on a mosquito sodium channel. | pyrethroid insecticides are widely used as one of the most effective control measures in the global fight against agricultural arthropod pests and mosquito-borne diseases, including malaria and dengue. they exert toxic effects by altering the function of voltage-gated sodium channels, which are essential for proper electrical signaling in the nervous system. a major threat to the sustained use of pyrethroids for vector control is the emergence of mosquito resistance to pyrethroids worldwide. her ... | 2013 | 23821746 |
| the developmental transcriptome of the mosquito aedes aegypti, an invasive species and major arbovirus vector. | mosquitoes are vectors of a number of important human and animal diseases. the development of novel vector control strategies requires a thorough understanding of mosquito biology. to facilitate this, we used rna-seq to identify novel genes and provide the first high-resolution view of the transcriptome throughout development and in response to blood feeding in a mosquito vector of human disease, aedes aegypti, the primary vector for dengue and yellow fever. we characterized mrna expression at 3 ... | 2013 | 23833213 |
| storm sewers as larval habitats for aedes aegypti and culex spp. in a neighborhood of merida, mexico. | we report the collection of aedes aegypti, culex quinquefasciatus, cx. interrogator, cx. thriambus, cx. coronator, and cx. salinarius larvae from storm sewers within an endemic area for dengue transmission in merida, mexico, during the rainy season of 2011. this is the first record of the dengue vector ae. aegypti breeding in storm sewers in the southeast of mexico. | 2012 | 23833907 |
| impact of argemone mexicana extracts on the cidal, morphological, and behavioral response of dengue vector, aedes aegypti l. (diptera: culicidae). | the larvicidal, behavioral, and morphological response of dengue vector, aedes aegypti treated with deleterious weed, argemone mexicana, was explored. the 1,000 ppm extracts of a. mexicana leaf, stem, and roots prepared in five different solvents (petroleum ether, hexane, benzene, acetone, and ethanol) were screened for their larvicidal activity against dengue vector establishing the efficacy of petroleum ether and hexane extracts. other extracts, unable to give 100% mortality, were considered i ... | 2013 | 23835923 |
| sustained reduction of the dengue vector population resulting from an integrated control strategy applied in two brazilian cities. | aedes aegypti has developed evolution-driven adaptations for surviving in the domestic human habitat. several trap models have been designed considering these strategies and tested for monitoring this efficient vector of dengue. here, we report a real-scale evaluation of a system for monitoring and controlling mosquito populations based on egg sampling coupled with geographic information systems technology. the smcp-aedes, a system based on open technology and open data standards, was set up fro ... | 2013 | 23844059 |
| cyclopaldic acid, seiridin, and sphaeropsidin a as fungal phytotoxins, and larvicidal and biting deterrents against aedes aegypti (diptera: culicidae): structure-activity relationships. | aedes aegypti l. is the major vector of the arboviruses responsible for dengue fever, one of the most devastating human diseases. from a preliminary screening of fungal phytotoxins, cyclopaldic acid (1), seiridin (2), sphaeropsidin a (4), and papyracillic acid (5) were evaluated for their biting deterrent and larvicidal activities against ae. aegypti l. because compounds 1, 2, 4, and 5 exhibited mosquito biting deterrent activities and 1 and 4 demonstrated larvicidal activities, further structur ... | 2013 | 23847068 |
| transcriptomic profiling of diverse aedes aegypti strains reveals increased basal-level immune activation in dengue virus-refractory populations and identifies novel virus-vector molecular interactions. | genetic variation among aedes aegypti populations can greatly influence their vector competence for human pathogens such as the dengue virus (denv). while intra-species transcriptome differences remain relatively unstudied when compared to coding sequence polymorphisms, they also affect numerous aspects of mosquito biology. comparative molecular profiling of mosquito strain transcriptomes can therefore provide valuable insight into the regulation of vector competence. we established a panel of a ... | 2013 | 23861987 |
| dengue in the united states of america: a worsening scenario? | dengue is a febrile illness caused by any of the four dengue virus types (denv-1 to -4, genus flavivirus, family flaviviridae) mainly transmitted by the mosquito aedes aegypti. denv can be transmitted by blood transfusion. dengue has been historically present in the continental united states (us), in the state of hawaii, and in the us insular territories in the caribbean and the pacific. during the second half of the 20th century, most of the cases reported in the us were imported cases brought ... | 2013 | 23865061 |
| development of guidelines for the surveillance of invasive mosquitoes in europe. | the recent notifications of autochthonous cases of dengue and chikungunya in europe prove that the region is vulnerable to these diseases in areas where known mosquito vectors (aedes albopictus and aedes aegypti) are present. strengthening surveillance of these species as well as other invasive container-breeding aedine mosquito species such as aedes atropalpus, aedes japonicus, aedes koreicus and aedes triseriatus is therefore required. in order to support and harmonize surveillance activities ... | 2013 | 23866915 |
| genetic and evolutionary analysis of cell-fusing agent virus based on thai strains isolated in 2008 and 2012. | increasing attention is being devoted to ecological and evolutionary relationships between insect-specific flaviviruses and globally important human-pathogenic flaviviruses such as dengue viruses. one such insect flavivirus, cell-fusing agent virus (cfav), remains poorly investigated. in this study, we isolated 13 and 16 cfav strains from aedes aegypti mosquitoes collected in thailand in 2008 and 2012, respectively, and performed genetic and evolutionary analyses based on gene regions encoding t ... | 2013 | 23871775 |
| an explosive epidemic of denv-3 in cairns, australia. | from november 2008-may 2009 cairns queensland australia was struck by an explosive epidemic of denv-3 that exceeded the capacity of highly skilled dengue control team to control it. we describe the environmental, virological and entomological factors associated with this outbreak to better understand the circumstances leading to its occurrence. patient interviews, serological results and viral sequencing strongly suggest that the imported index case was infected in kalimantan, indonesia. a delay ... | 2013 | 23874522 |
| stage-specific transcription during development of aedes aegypti. | aedes aegypti is the most important global vector of dengue virus infection in humans. availability of the draft genome sequence of this mosquito provides unique opportunities to study different aspects of its biology, including identification of genes and pathways relevant to the developmental processes associated with transition across individual life stages. however, detailed knowledge of gene expression patterns pertaining to developmental stages of a. aegypti is largely lacking. | 2013 | 23875547 |
| efficacy of various larvicides against aedes aegypti immatures in the laboratory. | we conducted a laboratory study to evaluate the efficacy of control agents against small larvae, large larvae, and pupae of aedes aegypti to determine an appropriate larvicide regime to employ in emergency dengue control programs. the control agents included bacillus thuringiensis var. israelensis (bti), pyriproxyfen (an insect growth regulator), a larvicidal oil, aquatain amf (polydimethylsiloxane, a monomolecular film), and temephos at the recommend application dosages and rates. our results s ... | 2013 | 23883850 |
| the sublethal effects of the entomopathic fungus leptolegnia chapmanii on some biological parameters of the dengue vector aedes aegypti. | the mosquito aedes aegypti (l.) (diptera: culicidae) is the primary vector of dengue in the americas. the use of chemical insecticides is recommended during outbreaks of dengue in order to reduce the number of adult mosquitoes; however, because ae. aegypti is highly synanthropic, the use of insecticides in densely populated areas is a dangerous practice. leptolegnia chapmanii seymour (straminipila: peronosporomycetes) is an entomopathogenic microorganism that has demonstrated marked pathogenicit ... | 0 | 23901823 |
| structure-activity relationships of monoterpenes and acetyl derivatives against aedes aegypti (diptera: culicidae) larvae. | dengue fever virus transmitted by aedes aegypti causes lethal mortalities of human beings, and, because of the lack of any vaccine, management of this vector, especially with phytochemicals, is essential. in the present investigation, the structure-activity relationship of monoterpenes and their acetyl derivatives was studied to identify structural features that are responsible for mosquitocidal activity. | 2013 | 23908002 |
| male-female differences in the number of reported incident dengue fever cases in six asian countries. | demographic factors, such as age and sex, are associated with the likelihood of exposure to aedes aegypti, the vector for dengue. however, dengue data disaggregated by both sex and age are not routinely reported or analysed by national surveillance systems. this study analysed the reported number of incident dengue cases by age and sex for six countries in asia. | 2011 | 23908884 |
| spatial analysis of aedes aegypti immatures in northern argentina: clusters and temporal instability. | the objective of this study was to analyze the spatio-temporal patterns of aedes aegypti immatures based on four entomological surveys that inspected over 6000 households in a large neighborhood of the city of clorinda between 2007 and 2008. global and local spatial point pattern analyses of immature presence or absence, habitat quality (estimated using a previously obtained statistical model) and pupal production were performed. global analyses showed aggregation of both infestation and habitat ... | 2013 | 23911331 |
| contrasting patterns of tolerance between chemical and biological insecticides in mosquitoes exposed to uv-a. | mosquitoes are vectors of major human diseases, such as malaria, dengue or yellow fever. because no efficient treatments or vaccines are available for most of these diseases, control measures rely mainly on reducing mosquito populations by the use of insecticides. numerous biotic and abiotic factors are known to modulate the efficacy of insecticides used in mosquito control. mosquito breeding sites vary from opened to high vegetation covered areas leading to a large ultraviolet gradient exposure ... | 2013 | 23911355 |
| phylogeography of aedes aegypti (yellow fever mosquito) in south florida: mtdna evidence for human-aided dispersal. | the invasive dengue vector aedes aegypti has persisted for > 200 years in south florida in the united states. we tested the hypotheses that florida's landscape creates dispersal barriers and corridors and that long-distance human-aided dispersal structures populations of ae. aegypti. we evaluated the phylogeography of 362 individuals from florida's east and west coasts with a 760-bp (418- and 342-bp fragments of nd5 and nd4, respectively) mitochondrial sequence. populations from these two coasts ... | 2013 | 23918216 |
| an improved autocidal gravid ovitrap for the control and surveillance of aedes aegypti. | limited success has been achieved using traditional vector control methods to prevent the transmission of dengue viruses. integrated control programs incorporating alternative tools, such as gravid ovitraps (lethal ovitraps and sticky ovitraps) may provide greater potential for monitoring and reducing vector populations and dengue virus transmission. we had developed an autocidal gravid ovitrap (ago) as a simple, low-cost device for surveillance and control of ae. aegypti without the use of pest ... | 2013 | 23919568 |
| history of aedes mosquitoes in hawaii. | as a geographically isolated island chain with no native mosquitoes, hawaii is a model for examining the mechanisms behind insect vector invasions and their subsequent interactions with each other and with human populations. the yellow fever mosquito, aedes aegypti, and the asian tiger mosquito, ae. albopictus, have been responsible for epidemics of dengue in hawaii. as one of the world's earliest locations to be invaded by both species, hawaii's history is particularly relevant because both spe ... | 2013 | 23923330 |
| mosquito protein kinase g phosphorylates flavivirus ns5 and alters flight behavior in aedes aegypti and anopheles gambiae. | many arboviral proteins are phosphorylated in infected mammalian cells, but it is unknown if the same phosphorylation events occur when insects are similarly infected. one of the mammalian kinases responsible for phosphorylation, protein kinase g (pkg), has been implicated in the behavior of multiple nonvector insects, but is unstudied in mosquitoes. pkg from aedes aegypti was cloned, and phosphorylation of specific viral sites was monitored by mass spectrometry from biochemical and cell culture ... | 2013 | 23930976 |
| reduced competitiveness of wolbachia infected aedes aegypti larvae in intra- and inter-specific immature interactions. | wolbachia are maternally inherited intracellular bacteria that frequently infect a diverse range of arthropod species. empirical and theoretical studies examining wolbachia invasiveness have emphasized wolbachia effects on adult hosts, but recent studies show that wolbachia impacts on immature hosts can be important also. here, we have examined for effects of wolbachia infection in aedes aegypti. specifically, differential survivorship is observed when young larvae (1st instar) are exposed to ol ... | 2013 | 23933013 |
| seasonal and nonseasonal dynamics of aedes aegypti in rio de janeiro, brazil: fitting mathematical models to trap data. | mathematical models suggest that seasonal transmission and temporary cross-immunity between serotypes can determine the characteristic multi-year dynamics of dengue fever. seasonal transmission is attributed to the effect of climate on mosquito abundance and within host virus dynamics. in this study, we validate a set of temperature and density dependent entomological models that are built-in components of most dengue models by fitting them to time series of ovitrap data from three distinct neig ... | 2014 | 23933186 |
| genetic mapping of specific interactions between aedes aegypti mosquitoes and dengue viruses. | specific interactions between host genotypes and pathogen genotypes (g×g interactions) are commonly observed in invertebrate systems. such specificity challenges our current understanding of invertebrate defenses against pathogens because it contrasts the limited discriminatory power of known invertebrate immune responses. lack of a mechanistic explanation, however, has questioned the nature of host factors underlying g×g interactions. in this study, we aimed to determine whether g×g interaction ... | 2013 | 23935524 |
| molecular and functional characterization of odorant-binding protein genes in an invasive vector mosquito, aedes albopictus. | aedes albopictus is a major vector of dengue and chikungunya viruses. olfaction plays a vital role in guiding mosquito behaviors and contributes to their ability to transmit pathogens. odorant-binding proteins (obps) are abundant in insect olfactory tissues and involved in the first step of odorant reception. while comprehensive descriptions are available of obps from aedes aegypti, culex quinquefasciatus and anopheles gambiae, only a few genes from ae. albopictus have been reported. in this stu ... | 2013 | 23935894 |
| isolation of midgut escape mutants of two american genotype dengue 2 viruses from aedes aegypti. | several studies have shown that american genotype dengue 2 viruses (denv2) have reduced viral fitness in the mosquito vector, aedes aegypti, compared to other denv2 genotypes. diminished replication efficiency or inability to efficiently traverse membrane barriers encompassing organs such as the midgut or salivary glands are considered major factors negatively impacting viral fitness in the mosquito. | 2013 | 23937713 |
| [genetic variability of aedes aegypti determined by mitochondrial gene nd4 analysis in eleven endemic areas for dengue in peru]. | in order to establish the genetic variability of aedes aegypti determined by the analysis of the mt-nd4 gene, in eleven endemic regions for dengue in peru, 51 samples of ae. aegypti were tested. the genetic variability was determined through the amplification and sequencing of a fragment of 336 base-pairs of mt nd4, the analysis of intra-specific phylogeny was conducted with the network ver. 4.6.10 program; and the phylogenetic analysis, with the neighbor joining distance method. the presence of ... | 2013 | 23949510 |
| dynamics and characterization of aedes aegypti (l.) (diptera: culicidae) key breeding sites. | the present study aimed to analyze the dynamics of containers used as breeding sites by aedes aegypti (l.) in the city of aracaju, se, one of the northeast brazilian states. a total of three entomological surveys were performed during different precipitation levels. breeding sites were categorized according to their function into storage, disposable containers, and reusable containers. "mean number of pupae" and "frequency of each type of breeding site" were the criteria considered to identify k ... | 2013 | 23949815 |
| morphological changes in the midgut of aedes aegypti l. (diptera: culicidae) larvae following exposure to an annona coriacea (magnoliales: annonaceae) extract. | bioinsecticides are important in the control of disease vectors, but data regarding their physiological effects on target insects are incomplete. this study describes morphological changes that occur in the midgut of third instar aedes aegypti l. (diptera: culicidae) following treatment with a methanolic extract of annona coriacea (magnoliales: annonaceae). dissected midguts were subdivided into anterior and posterior regions and analyzed by light and scanning electron microscopy. insects expose ... | 2012 | 23950067 |
| wolbachia-associated bacterial protection in the mosquito aedes aegypti. | wolbachia infections confer protection for their insect hosts against a range of pathogens including bacteria, viruses, nematodes and the malaria parasite. a single mechanism that might explain this broad-based pathogen protection is immune priming, in which the presence of the symbiont upregulates the basal immune response, preparing the insect to defend against subsequent pathogen infection. a study that compared natural wolbachia infections in drosophila melanogaster with the mosquito vector ... | 2013 | 23951381 |
| life table characteristics of aedes aegypti (diptera:culicidae) from saudi arabia. | aedes aegypti (= stegomyia aegypti) mosquito is a world vector of important arboviral diseases like dengue and rift valley fever. despite its wide distribution in the western and southern regions of saudi arabia, where dengue outbreaks have occurred, its ecology is largely unknown. in this study we report on the main life table developmental attributes of a laboratory colony of ae. aegypti reared from field-collected larvae from madinah province, west of saudi arabia. females were maintained on ... | 2013 | 23959496 |
| mosquito larvicidal activity of aloe vera (family: liliaceae) leaf extract and bacillus sphaericus, against chikungunya vector, aedes aegypti. | the bio-efficacy of aloe vera leaf extract and bacterial insecticide, bacillus sphaericus larvicidal activity was assessed against the first to fourth instars larvae of aedes aegypti, under the laboratory conditions. the plant material was shade dried at room temperature and powdered coarsely. a. vera and b. sphaericus show varied degrees of larvicidal activity against various instars larvae of a. aegypti. the lc50 of a. vera against the first to fourth instars larvae were 162.74, 201.43, 253.30 ... | 2012 | 23961212 |
| vector competence of aedes albopictus and aedes aegypti (diptera: culicidae) for den2-43 and new guinea c virus strains of dengue 2 virus. | the vector competence of aedes albopictus and aedes aegypti with regard to den2-43 and new guinea c (ngc) virus strains of dengue 2 viruses was assessed and compared. the infection and dissemination rate and distribution of den2-43 antigens in orally infected ae. albopictus was investigated using the reverse transcription polymerase chain reaction and an indirect immunofluorescence assay. to better understand the initial infection, dissemination and transmission of these viral strains in vector ... | 2013 | 23962388 |
| chikungunya and dengue autochthonous cases in europe, 2007-2012. | a large number of autochthonous cases of dengue fever (2237) and chikungunya fever (231) occurred in europe (italy, france, croatia, madeira) during the period covered by our analysis (2007-2012). in all dengue outbreaks, the circulating strain, identified by means of molecular analysis, was the denv-1 strain. dengue and chikungunya are infectious diseases that often result in hospitalizations and are associated with high public health costs. the dengue epidemic on the island of madeira resulted ... | 2014 | 23962447 |
| using global maps to predict the risk of dengue in europe. | this article attempts to quantify the risk to europe of dengue, following the arrival and spread there of one of dengue's vector species aedes (stegomyia) albopictus. a global risk map for dengue is presented, based on a global database of the occurrence of this disease, derived from electronic literature searches. remotely sensed satellite data (from nasa's modis series), interpolated meteorological data, predicted distribution maps of dengue's two main vector species, aedes aegypti and aedes a ... | 2014 | 23973561 |
| modeling the non-stationary climate dependent temporal dynamics of aedes aegypti. | temperature and humidity strongly affect the physiology, longevity, fecundity and dispersal behavior of aedes aegypti, vector of dengue fever. contrastingly, the statistical associations measured between time series of mosquito abundance and meteorological variables are often weak and contradictory. here, we investigated the significance of these relationships at different time scales. | 2013 | 23976939 |
| silencing of p-glycoprotein increases mortality in temephos-treated aedes aegypti larvae. | re-emergence of vector-borne diseases such as dengue and yellow fever, which are both transmitted by the aedes aegypti mosquito, has been correlated with insecticide resistance. p-glycoproteins (p-gps) are atp-dependent efflux pumps that are involved in the transport of substrates across membranes. some of these proteins have been implicated in multidrug resistance (mdr). in this study, we identified a putative p-glycoprotein in the ae. aegypti database based on its significantly high identity w ... | 2013 | 23980723 |
| bioactivity of plant extracts on the larval and pupal stages of aedes aegypti (diptera, culicidea). | aedes aegypti is responsible for the transmission of the dengue and yellow fever viruses. this study evaluated the effects of extracts from cnidosculos phyllacanthus, ricinus communis, and coutarea hexandra on the developmental periods of a.aegypti larvae and pupae. crude extracts of c. phyllacanthus and c. hexandra and oil from r. communis and c. phyllacanthus were used. | 2013 | 23982096 |
| the toll and imd pathways are not required for wolbachia-mediated dengue virus interference. | wolbachia blocks dengue virus replication in drosophila melanogaster as well as in aedes aegypti. using the drosophila model and mutations in the toll and imd pathways, we showed that neither pathway is required for expression of the dengue virus-blocking phenotype in the drosophila host. this provides additional evidence that the mechanistic basis of wolbachia-mediated dengue virus blocking in insects is more complex than simple priming of the host insect innate immune system. | 2013 | 23986574 |
| formulas of components of citronella oil against mosquitoes (aedes aegypti). | the mosquito aedes aegypti is an epidemic vector of several diseases such as dengue fever and yellow fever. several pesticides are used to control the mosquito population. because of their frequent use, some mosquitoes have developed resistance. in this study, we used the y-tube olfactometer to test essential oils of cymbopogon species and screened specific formulas of components as repellents against ae. aegypti. at 400 μl, the extracted oil of citronella grass (cymbopogon nardus) and myrcene p ... | 2013 | 23998314 |
| risk factors related to dengue infections in primary school students: exploring students' basic knowledge of dengue and examining the larval indices in southern thailand. | dengue is a major problem in southern thailand. | 2013 | 23999339 |
| turning cigarette butt waste into an alternative control tool against an insecticide-resistant mosquito vector. | annually, 4.5 trillion cigarette butts (cbs) are flicked into our environment. evidence exists that cb waste is deadly to aquatic life, but their lethality to the aquatic life of the main dengue vector is unknown. cbs are full of toxicants that occur naturally, during planting and manufacturing, which may act as larvicidal agents. we assessed aedes aegypti vulnerability to marlboro butts during its development. overall, cbs showed insecticidal activities against larvae. at early phases of develo ... | 2013 | 23999373 |
| spatial clustering of aedes aegypti related to breeding container characteristics in coastal ecuador: implications for dengue control. | mosquito management within households remains central to the control of dengue virus transmission. an important factor in these management decisions is the spatial clustering of aedes aegypti. we measured spatial clustering of ae. aegypti in the town of borbón, ecuador and assessed what characteristics of breeding containers influenced the clustering. we used logistic regression to assess the spatial extent of that clustering. we found strong evidence for juvenile mosquito clustering within 20 m ... | 2013 | 24002483 |
| resting behaviour of aedes aegypti in trinidad: with evidence for the re-introduction of indoor residual spraying (irs) for dengue control. | historically, aedes aegypti (l.) mosquitoes were controlled/eradicated by fumigation, residual spraying and the elimination of breeding sites. however, the underlying mechanisms of how these vector populations were managed have never been evaluated. most studies report that these programs failed due to the emergence of ddt resistance in the 1950s and early 1960s. therefore, behavioural and physiological factors have never been examined to determine program success or failure. | 2013 | 24004641 |
| green synthesis of silver nanoparticles using sida acuta (malvaceae) leaf extract against culex quinquefasciatus, anopheles stephensi, and aedes aegypti (diptera: culicidae). | mosquitoes act as a vector for most of the life-threatening diseases like malaria, yellow fever, dengue fever, chikungunya fever, filariasis, encephalitis, west nile virus infection, etc. under the integrated mosquito management, emphasis was given on the application of alternative strategies in mosquito control. the continuous application of synthetic insecticides causes development of resistance in vector species, biological magnification of toxic substances through the food chain, and adverse ... | 2013 | 24005479 |
| mosquito repellents based on a natural chromene analogue with longer duration of action than n,n-diethyl-meta-toluamide (deet). | mosquito repellents play a major role in reducing bites and therefore mitigating transmission of mosquito-borne diseases. there is concern by some about the reported neurotoxic effects of the popular repellent deet. also, a product with longer effective activity after application is needed. this paper describes the synthesis and repellent activity of (2,2 dimethyl-2h-chromen-5-yl)methanol, a derivative of chromene amide that is a compound from the plant amyris texana . this compound is more pote ... | 2013 | 24006960 |
| a global airport-based risk model for the spread of dengue infection via the air transport network. | the number of travel-acquired dengue infections has seen a consistent global rise over the past decade. an increased volume of international passenger air traffic originating from regions with endemic dengue has contributed to a rise in the number of dengue cases in both areas of endemicity and elsewhere. this paper reports results from a network-based risk assessment model which uses international passenger travel volumes, travel routes, travel distances, regional populations, and predictive sp ... | 2013 | 24009672 |
| testing fungus impregnated cloths for the control of adult aedes aegypti under natural conditions. | entomopathogenic fungi could be useful tools for reducing populations of the dengue mosquito aedes aegypti. here the efficiency of fungus (metarhizium anisopliae) impregnated cloths (with and without imidacloprid [imi]) was evaluated against adult a. aegypti in simulated human dwellings. behaviour of mosquitoes in the presence of black cloths was also investigated. | 2013 | 24010874 |
| eco-friendly microbial route to synthesize cobalt nanoparticles using bacillus thuringiensis against malaria and dengue vectors. | the developments of resistance and persistence to chemical insecticides and concerns about the non-target effects have prompted the development of eco-friendly mosquito control agents. the aim of this study was to investigate the larvicidal activities of synthesized cobalt nanoparticles (co nps) using bio control agent, bacillus thuringiensis against malaria vector, anopheles subpictus and dengue vector, aedes aegypti (diptera: culicidae). the synthesized co nps were characterized by x-ray diffr ... | 2013 | 24013343 |
| comparison of the mosquito inoculation technique and quantitative real time polymerase chain reaction to measure dengue virus concentration. | an accurate measure of infectious dengue virus in human and mosquito tissues is critical to fully understand virus-host relationships, disease severity, viral fitness, and pathogenesis. in recent years, rna copy number measured by quantitative real time-polymerase chain reaction has been used to measure dengue virus concentration in vitro and in vivo. in this study, we detail important differences in the measurement of viral growth kinetics in vero and c6/36 tissue cultures, in aedes aegypti mos ... | 2013 | 24019432 |
| a reduce and replace strategy for suppressing vector-borne diseases: insights from a deterministic model. | genetic approaches for controlling disease vectors have aimed either to reduce wild-type populations or to replace wild-type populations with insects that cannot transmit pathogens. here, we propose a reduce and replace (r&r) strategy in which released insects have both female-killing and anti-pathogen genes. we develop a mathematical model to numerically explore release strategies involving an r&r strain of the dengue vector aedes aegypti. we show that repeated r&r releases may lead to a tempor ... | 2013 | 24023839 |
| confusion, knock-down and kill of aedes aegypti using metofluthrin in domestic settings: a powerful tool to prevent dengue transmission? | dengue control methods are reliant upon control of the vector, primarily aedes aegypti. current adulticiding methods in north queensland include treating premises with residual synthetic pyrethroid insecticides (interior residual spraying; irs), a laborious, intrusive task. the vapor active synthetic pyrethroid metofluthrin might offer an efficient alternative as some studies indicate that it prevents biting and has strong knockdown effects. however, its expellant and/or irritant effects, longev ... | 2013 | 24025232 |
| requirement for commissureless2 function during dipteran insect nerve cord development. | in drosophila melanogaster, commissureless (comm) function is required for proper nerve cord development. although comm orthologs have not been identified outside of drosophila species, some insects possess orthologs of drosophila comm2, which may also regulate embryonic nerve cord development. here, this hypothesis is explored through characterization of comm2 genes in two disease vector mosquitoes. | 2013 | 24026811 |
| abundance and prevalence of aedes aegypti immatures and relationships with household water storage in rural areas in southern viet nam. | since 2000, the government of viet nam has committed to provide rural communities with increased access to safe water through a variety of household water supply schemes (wells, ferrocement tanks and jars) and piped water schemes. one possible, unintended consequence of these schemes is the concomitant increase in water containers that may serve as habitats for dengue mosquito immatures, principally aedes aegypti. to assess these possible impacts we undertook detailed household surveys of ae. ae ... | 2011 | 24038184 |
| promising aedes aegypti repellent chemotypes identified through integrated qsar, virtual screening, synthesis, and bioassay. | molecular field topology analysis, scaffold hopping, and molecular docking were used as complementary computational tools for the design of repellents for aedes aegypti, the insect vector for yellow fever, chikungunya, and dengue fever. a large number of analogues were evaluated by virtual screening with glide molecular docking software. this produced several dozen hits that were either synthesized or procured from commercial sources. analysis of these compounds by a repellent bioassay resulted ... | 2013 | 24039693 |
| comparison of mosquito densoviruses: two clades of viruses isolated from indigenous mosquitoes. | we analyzed the phylogenetic tree of densoviruses isolated from indigenous mosquitoes and mosquito cell lines. our findings suggest two distinct clades of densovirus. the viruses in the first clade were isolated from an indigenous mosquito which had the aedes aegypti densovirus (aaednv) as a representative virus. the other clade of viruses was isolated from mosquito indigenous cell line which had the aedes albopictus densovirus (aaldnv) as the representative virus. the origin of the two clades o ... | 2013 | 24050091 |
| detection of the v1016g mutation in the voltage-gated sodium channel gene of aedes aegypti (diptera: culicidae) by allele-specific pcr assay, and its distribution and effect on deltamethrin resistance in thailand. | resistance to pyrethroid insecticides is widespread among populations of aedes aegypti, the main vector for the dengue virus. several different point mutations within the voltage-gated sodium channel (vgsc) gene contribute to such resistance. a mutation at position 1016 in domain ii, segment 6 of the vgsc gene in ae. aegypti leads to a valine to glycine substitution (v1016g) that confers resistance to deltamethrin. | 2013 | 24059267 |
| temephos resistance in aedes aegypti in colombia compromises dengue vector control. | control and prevention of dengue relies heavily on the application of insecticides to control dengue vector mosquitoes. in colombia, application of the larvicide temephos to the aquatic breeding sites of aedes aegypti is a key part of the dengue control strategy. resistance to temephos was recently detected in the dengue-endemic city of cucuta, leading to questions about its efficacy as a control tool. here, we characterize the underlying mechanisms and estimate the operational impact of this re ... | 2013 | 24069492 |
| imperviousness as a predictor for infestation levels of container-breeding mosquitoes in a focus of dengue and saint louis encephalitis in argentina. | dengue and saint louis encephalitis virus are among the most important emerging viruses transmitted by mosquitoes at the global scale, and from 2009 onward both diseases have reached temperate argentina. to test whether the urbanization level can be used as a predictor for the infestation levels of container-breeding mosquito vectors, we searched for aedes aegypti and culex pipiens in 8400 water-filled containers from 14 cemeteries of buenos aires province and we used generalized linear models t ... | 2013 | 24071380 |
| genetic structure of aedes aegypti populations determined using pairwise comparisons. | the biological characteristics of aedes aegypti (diptera, culicidae), which is a vector of dengue and yellow fever, make this organism a good model for studying population structure and the events that may influence it under the effect of human activity. we assessed the genetic variability of five a. aegypti populations using rapd-pcr technique and six primers. four populations were from brazil and one was from the usa. a total of 165 polymorphic dna loci were generated. considering the six prim ... | 2013 | 24085439 |
| the enzyme 3-hydroxykynurenine transaminase as potential target for 1,2,4-oxadiazoles with larvicide activity against the dengue vector aedes aegypti. | the mosquito aedes aegypti is the vector agent responsible for the transmission of yellow fever and dengue fever viruses to over 80 million people in tropical and subtropical regions of the world. exhaustive efforts have lead to a vaccine candidate with only 30% effectiveness against the dengue virus and failure to protect patients against the serotype 2. hence, vector control remains the most viable route to dengue fever control programs. we have synthesized a class of 1,2,4-oxadiazole derivati ... | 2013 | 24095017 |
| human impacts have shaped historical and recent evolution in aedes aegypti, the dengue and yellow fever mosquito. | although anthropogenic impacts are often considered harmful to species, human modifications to the landscape can actually create novel niches to which other species can adapt. these "domestication" processes are especially important in the context of arthropod disease vectors, where ecological overlap of vector and human populations may lead to epidemics. here, we present results of a global genetic study of one such species, the dengue and yellow fever mosquito, aedes aegypti, whose evolutionar ... | 2013 | 24111703 |
| functional and genetic characterization of neuropeptide y-like receptors in aedes aegypti. | female aedes aegypti mosquitoes are the principal vector for dengue fever, causing 50-100 million infections per year, transmitted between human and mosquito by blood feeding. ae. aegypti host-seeking behavior is known to be inhibited for three days following a blood meal by a hemolymph-borne humoral factor. head peptide-i is a candidate peptide mediating this suppression, but the mechanism by which this peptide alters mosquito behavior and the receptor through which it signals are unknown. | 2013 | 24130914 |
| mosquito saliva serine protease enhances dissemination of dengue virus into the mammalian host. | dengue virus (denv), a flavivirus of global importance, is transmitted to humans by mosquitoes. in this study, we developed in vitro and in vivo models of saliva-mediated enhancement of denv infectivity. serine protease activity in aedes aegypti saliva augmented virus infectivity in vitro by proteolyzing extracellular matrix proteins, thereby increasing viral attachment to heparan sulfate proteoglycans and inducing cell migration. a serine protease inhibitor reduced saliva-mediated enhancement o ... | 2013 | 24131723 |
| distribution, seasonal variation & dengue transmission prediction in sisaket, thailand. | environmental factors including weather variables may play a significant role in the transmission of dengue. this study investigated the effect of seasonal variation on the abundance of aedes aegypti and ae. albopictus larvae and explored the impact of weather variability on dengue transmission in sisaket, thailand. | 0 | 24135179 |
| aedes aegypti ml and niemann-pick type c family members are agonists of dengue virus infection. | upon exposure to dengue virus, the aedes aegypti mosquito vector mounts an anti-viral immune defense by activating the toll, jak/stat, and rnai pathways, thereby limiting infection. while these pathways and several other factors have been identified as dengue virus antagonists, our knowledge of factors that facilitate dengue virus infection is limited. previous dengue virus infection-responsive transcriptome analyses have revealed an increased mrna abundance of members of the myeloid differentia ... | 2013 | 24135719 |
| neurological complications in dengue infection: a review for clinical practice. | dengue is an important global public health problem. the world health organization estimates that 2/5 of entire world population are in risk of dengue infection. almost 50 millions cases occur annually, with at least 20 thousand deaths. the etiological agent of this acute febrile disease is a single-strand positive-sense rna virus of flavivirus genus. it is an arboviral disease transmitted by aedes sp. mosquitoes (aedes aegypti and a. albopictus). most infected individuals present asymptomatic i ... | 2013 | 24141501 |
| first isolation of dengue virus from the 2010 epidemic in nepal. | dengue is an emerging disease in nepal and was first observed as an outbreak in nine lowland districts in 2006. in 2010, however, a large epidemic of dengue occurred with 4,529 suspected and 917 serologically-confirmed cases and five deaths reported in government hospitals in nepal. the collection of demographic information was performed along with an entomological survey and clinical evaluation of the patients. a total of 280 serum samples were collected from suspected dengue patients. these sa ... | 2013 | 24155651 |
| gustatory receptor expression in the labella and tarsi of aedes aegypti. | the yellow-fever mosquito, aedes aegypti, infects a growing number of people every year with dengue, yellow fever and chikungunya viruses. contact chemoreception in mosquitoes influences a number of behaviors including host-selection, oviposition and feeding. while these behaviors are in many instances well documented, the molecular mechanisms mediating them are not well understood. here we report the results of sequencing total messenger rna in the labella and tarsi of both male and female ae. ... | 2013 | 24157615 |
| strong larvicidal potential of artemisia annua leaf extract against malaria (anopheles stephensi liston) and dengue (aedes aegypti l.) vectors and bioassay-driven isolation of the marker compounds. | malaria and dengue are the two most important vector-borne human diseases caused by mosquito vectors anopheles stephensi and aedes aegypti, respectively. of the various strategies adopted for eliminating these diseases, controlling of vectors through herbs has been reckoned as one of the important measures for preventing their resurgence. artemisia annua leaf chloroform extract when tried against larvae of a. stephensi and a. aegypti has shown a strong larvicidal activity against both of these v ... | 2014 | 24158647 |
| discriminable roles of aedes aegypti and aedes albopictus in establishment of dengue outbreaks in taiwan. | aedes aegypti and aedes albopictus were reported to be significant as vectors of dengue fever. in taiwan, the latter is distributed throughout the island while the former appears only south of the tropic of cancer; i.e., 23.5°n. in the past decade, there were five outbreaks with over 1000 cases of dengue fever in taiwan. without exception, these outbreaks all occurred in the south where the two aedes mosquitoes are sympartic. according to the center for disease control of taiwan, imported cases ... | 2014 | 24161880 |
| probing functional polymorphisms in the dengue vector, aedes aegypti. | dengue is the most prevalent arboviral disease world-wide and its primary vector is the mosquito aedes aegypti. the current lack of commercially-available vaccines makes control of vector populations the only effective strategy to prevent dengue transmission. aedes aegypti geographic populations exhibit great variability in insecticide resistance and susceptibility to dengue infection. the characterization of single nucleotide polymorphisms (snps) as molecular markers to study quantitatively thi ... | 2013 | 24168143 |
| biosynthesis of silver nanoparticles using bacillus thuringiensis against dengue vector, aedes aegypti (diptera: culicidae). | the present study reveals the larvicidal activity of silver nanoparticles (agnps) synthesized by bacillus thuringiensis (bt) against aedes aegypti responsible for the diseases of public health importance. the bt-agnps were characterized by using uv-visible spectrophotometer followed by scanning electron microscopy (sem) and energy-dispersive x-ray (edx) spectroscopy. a surface plasmon resonance spectrum of agnps was obtained at 420 nm. the particle sizes were measured through sem imaging ranging ... | 2014 | 24173811 |
| behavioral responses of two dengue virus vectors, aedes aegypti and aedes albopictus (diptera: culicidae), to duet and its components. | ultralow volume droplets of duet, prallethrin, and sumithrin at a sublethal dose were applied to unfed (nonbloodfed) and bloodfed female aedes aegypti l. and aedes albopictus (skuse) in a wind tunnel. control spray droplets only contained inert ingredients. individual mosquitoes were videotaped before, during, and after spraying and various behaviors analyzed. during the spray periods of all three pesticide treatments, mosquitoes spent a greater percentage of time moving, and the distance moved ... | 2013 | 24180111 |
| investigating the genetics of bti resistance using mrna tag sequencing: application on laboratory strains and natural populations of the dengue vector aedes aegypti. | mosquito control is often the main method used to reduce mosquito-transmitted diseases. in order to investigate the genetic basis of resistance to the bio-insecticide bacillus thuringiensis subsp. israelensis (bti), we used information on polymorphism obtained from cdna tag sequences from pooled larvae of laboratory bti-resistant and susceptible aedes aegypti mosquito strains to identify and analyse 1520 single nucleotide polymorphisms (snps). of the 372 snps tested, 99.2% were validated using d ... | 2013 | 24187584 |
| seasonal prevalence of aedes aegypti in urban and industrial areas of dibrugarh district, assam. | in dibrugarh district, one of the most populous districts of assam, there is a potential risk of dengue virus transmission by the mosquito aedes aegypti during the autumn, in the urban and industrial areas of the district. the seasonal patterns of oviposition activity and abundance of breeding sites of this vector were studied in two areas of the district. from january 2010 to december 2010, we examined a total of 767 ovitraps and 6562 water filled containers. both study areas showed high percen ... | 2013 | 24189673 |
| analysis of early dengue virus infection in mice as modulated by aedes aegypti probing. | dengue virus (denv), the etiologic agent of dengue fever, is transmitted during probing of human skin by infected-mosquito bite. the expectorated viral inoculum also contains an assortment of mosquito salivary proteins that have been shown to modulate host hemostasis and innate immune responses. to examine the potential role of mosquito probing in denv establishment within the vertebrate host, we inoculated mice intradermally with denv serotype 2 strain 1232 at sites where aedes aegypti had or h ... | 2013 | 24198426 |
| mosquito vector biology and control in latin america--a 23rd symposium. | the 23rd annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 79th annual meeting in atlantic city, nj, in february 2013. the principal objective, as for the previous 22 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 49 presentations that were given orally in spanish or presented as posters by partic ... | 2013 | 24199500 |
| indoor volatiles of primary school classrooms in tapachula, chiapas, mexico, are attractants to aedes aegypti females. | we determined the behavioral response of aedes aegypti females to volatile compounds collected in indoor primary school classrooms. volatiles were collected from classrooms from 0800 through 1030 h and 1130 through 1400 h in urban and rural schools in tapachula, chiapas, mexico. female responses to volatiles were assessed in a y-tube olfactometer. chemical compounds were identified using gas chromatography-mass spectrometer analysis. volatiles from both schools were attractive when compared agai ... | 2013 | 24199507 |
| the distribution of synonymous codon choice in the translation initiation region of dengue virus. | dengue is the most common arthropod-borne viral (arboviral) illness in humans. the genetic features concerning the codon usage of dengue virus (denv) were analyzed by the relative synonymous codon usage, the effective number of codons and the codon adaptation index. the evolutionary distance between denv and the natural hosts (homo sapiens, pan troglodytes, aedes albopictus and aedes aegypti) was estimated by a novel formula. finally, the synonymous codon usage preference for the translation ini ... | 2013 | 24204777 |
| insecticide-driven patterns of genetic variation in the dengue vector aedes aegypti in martinique island. | effective vector control is currently challenged worldwide by the evolution of resistance to all classes of chemical insecticides in mosquitoes. in martinique, populations of the dengue vector aedes aegypti have been intensively treated with temephos and deltamethrin insecticides over the last fifty years, resulting in heterogeneous levels of resistance across the island. resistance spreading depends on standing genetic variation, selection intensity and gene flow among populations. to determine ... | 2013 | 24204999 |
| mosquito vector diversity across habitats in central thailand endemic for dengue and other arthropod-borne diseases. | recent years have seen the greatest ecological disturbances of our times, with global human expansion, species and habitat loss, climate change, and the emergence of new and previously-known infectious diseases. biodiversity loss affects infectious disease risk by disrupting normal relationships between hosts and pathogens. mosquito-borne pathogens respond to changing dynamics on multiple transmission levels and appear to increase in disturbed systems, yet current knowledge of mosquito diversity ... | 2013 | 24205420 |
| dispersal of aedes aegypti: field study in temperate areas using a novel method. | since aedes aegypti was identified as vector of yellow fever and dengue, its dispersal is relevant for disease control. we studied the dispersal of ae. aegypti in temperate areas of argentina during egglaying, using the existing population and egg traps. | 2013 | 24220074 |
| increased container-breeding mosquito risk owing to drought-induced changes in water harvesting and storage in brisbane, australia. | extended drought conditions in south-east queensland during the early 2000s have resulted in a culture of water harvesting and legislated water restrictions. aedes notoscriptus is a container-breeding mosquito vector of ross river and barmah forest viruses. | 2013 | 24225151 |
| microrna levels are modulated in aedes aegypti after exposure to dengue-2. | to define microrna (mirna) involvement during arbovirus infection of aedes aegypti, we mined deep sequencing libraries of dengue type 2 (denv2)-exposed mosquitoes. three biological replicates for each timepoint [2, 4 and 9 days post-exposure (dpe)] and treatment group allowed us to remove the outliers associated with sample-to-sample variability. using edger (r bioconductor), designed for use with replicate deep sequencing data, we determined the log fold-change (logfc) of mirna levels [18-23 nu ... | 2013 | 24237456 |