Publications

isotopologue profiling of the listerial n-metabolism.	the nitrogen (n-) sources and the relative contribution of a nitrogenous nutrient to the n-pool of the gram-positive pathogen listeria monocytogenes are largely unknown. therefore, (15) n-isotopologue profiling was established to study the n-metabolism of l. monocytogenes. the pathogen was grown in a defined minimal medium supplemented with potential (15) n-labeled nutrients. the bacteria were harvested and hydrolysed under acidic conditions, and the resulting amino acids were analysed by gc-ms, ...	2016	26699934
comparison of antimicrobial activities of newly obtained low molecular weight scorpion chitosan and medium molecular weight commercial chitosan.	in this study the antimicrobial activity of low molecular weight (3.22 kda) chitosan, obtained for the first time from a species belonging to the scorpiones, was screened against nine pathogenic microorganisms (seven bacteria and two yeasts) and compared with that of medium molecular weight commercial chitosan (mmwcc). it was observed that the antimicrobial activity of the low molecular weight scorpion chitosan (lmwsc) was specific to bacterial species in general rather than gram-negative or gra ...	2016	26702952
microbial inactivation kinetics and mechanisms of carbon-doped tio2 (c-tio2) under visible light.	in this study, titanium dioxide nanoparticles doped with carbon (c-tio2) were synthesized by means of sol-gel methods, and the synthesis was verified by means of x-ray photoelectron spectroscopy. the nanoparticles' photocatalytic disinfection activity of listeria monocytogenes was tested under uv and visible light. the observed inactivation levels for 150min of visible light exposure with and without uv cutoff filters were 2.10 and 2.45 log, respectively. we also found that traditional reactive ...	2016	26705890
creative lysins: listeria and the engineering of antimicrobial enzymes.	cell wall lytic enzymes have been of increasing interest as antimicrobials for targeting gram-positive spoilage and pathogenic bacteria, largely due to the development of strains resistant to antibiotics and bacteriophage therapy. such lysins show considerable promise against listeria monocytogenes, a primary concern in food-processing environments, but there is room for improvement via protein engineering. advances in antilisterial applications could benefit from recent developments in lysin bi ...	2016	26710271
screening and characterization of potential bacillus starter cultures for fermenting low-salt soybean paste (doenjang).	the bacterial strains were screened as potential starters for fermenting low-salt doenjang (a korean traditional fermented soybean paste) using korean doenjang based on proteolytic and antipathogenic activities under 6.5-7.5% nacl conditions. phylogenetic analysis based on 16s rrna gene sequences showed that they all belonged to the genus bacillus. proteolytic and antipathogenic activities against escherichia coli, bacillus cereus, staphylococcus aureus, listeria monocytogenes, and aspergillus f ...	2016	26718466
evaluation of anti-bacterial effects of some novel thiazole and imidazole derivatives against some pathogenic bacteria.	bacterial resistance to antibiotics has motivated the researchers to evaluate the novel anti-bacterial compounds such as some thiazole and imidazole derivatives. thereby, in this work, we investigated the anti-bacterial effects of one new thiazole and two new imidazole derivatives on bacillus cereus, listeria monocytogenes, escherichia coli, salmonella typhimurium, proteus mirabilis and shigella dysenteriae.	2015	26719785
behavior of escherichia coli o157:h7 and listeria monocytogenes during fermentation and storage of camel yogurt.	in addition to its nutritional and therapeutic properties, camel milk has the ability to suppress the growth of a wide range of foodborne pathogens, but there is a lack of information regarding the behavior of these pathogens in products such as yogurt produced from camel milk. the objective of the current study was to investigate the behavior of listeria monocytogenes and escherichia coli o157:h7 during manufacture and storage of camel yogurt. camel milk inoculated with l. monocytogenes and e. ...	2016	26723116
effect of peracetic acid on biofilms formed by staphylococcus aureus and listeria monocytogenes isolated from dairy plants.	this research investigated the removal of adherent cells of 4 strains of staphylococcus aureus and 1 listeria monocytogenes strain (previously isolated from dairy plants) from polystyrene microtiter plates using peracetic acid (paa, 0.5%) for 15, 30, 60, and 120 s, and the inactivation of biofilms formed by those strains on stainless steel coupons using the same treatment times. in the microtiter plates, paa removed all s. aureus at 15 s compared with control (no paa treatment). however, l. mono ...	2016	26723125
differential internalin a levels in biofilms of listeria monocytogenes grown on different surfaces and nutrient conditions.	listeria monoctyogenes is a foodborne pathogen containing the surface protein, internalin a (inla). the expression of this protein permits the invasion of l. monocytogenes into intestinal epithelial cells expressing the receptor e-cadherin, thus crossing the intestinal barrier and resulting in listerosis. the main aim of this work was to investigate inla levels in different l. monocytogenes strains in both planktonic and sessile states using an anti-inla antibody. biofilms were grown in high and ...	2016	26724402
the role of listeria monocytogenes cell wall surface anchor protein lapb in virulence, adherence, and intracellular replication.	lmof2365_2117 is a listeria monocytogenes putative cell wall surface anchor protein with a conserved domain found in collagen binding proteins. we constructed a deletion mutation in lmof2365_2117 in serotype 4b strain f2365, evaluated its virulence, and determined its ability to adhere and invade colonic epithelial cells and macrophages. in a/j mice, colonization of liver was significantly higher for f2365 than for f2365δ2117. the ability of f2365δ2117 to adhere to caco-2 cells was significantly ...	2016	26724740
electrochemical genosensing of salmonella, listeria and escherichia coli on silica magnetic particles.	a magneto-genosensing approach for the detection of the three most common pathogenic bacteria in food safety, such as salmonella, listeria and escherichia coli is presented. the methodology is based on the detection of the tagged amplified dna obtained by single-tagging pcr with a set of specific primers for each pathogen, followed by electrochemical magneto-genosensing on silica magnetic particles. a set of primers were selected for the amplification of the inva (278 bp), prfa (217 bp) and eaea ...	2016	26724759
antimicrobial activities, toxicity and phenolic composition of asphodeline anatolica e. tuzlaci leaf extracts from turkey.	the antimicrobial activity of acetone, methanol and aqueous extracts of asphodeline anatolica e. tuzlaci leaves was evaluated against american type culture collection, food and clinical isolates (listeria monocytogenes and staphylococcus aureus including methicillin-resistant strains-mrsa). biofilm formation, toxicity and characterisation of the polyphenolic content were analysed. the acetone extract demonstrated a higher antibacterial activity against s. aureus including mrsa strains, l. monocy ...	2016	26727992
dnase-sensitive and -resistant modes of biofilm formation by listeria monocytogenes.	listeria monocytogenes is able to form biofilms on various surfaces and this ability is thought to contribute to persistence in the environment and on contact surfaces in the food industry. extracellular dna (edna) is a component of the biofilm matrix of many bacterial species and was shown to play a role in biofilm establishment of l. monocytogenes. in the present study, the effect of dnasei treatment on biofilm formation of l. monocytogenes egd-e was investigated under static and dynamic condi ...	2015	26733972
assessment of listeria sp. interference using a molecular assay to detect listeria monocytogenes in food.	detection of listeria monocytogenes in food is currently based on enrichment methods. when l. monocytogenes is present with other listeria species in food, the species compete during the enrichment process. overgrowth competition of the nonpathogenic listeria species might result in false-negative results obtained with the current reference methods. this potential issue was noted when 50 food samples artificially spiked with l. monocytogenes were tested with a real-time pcr assay and canada's cu ...	2016	26735040
molecular serogrouping of listeria monocytogenes from brazil using pcr.	we assessed the serotype distribution of listeria monocytogenes isolates from clinical, beef, and environment samples using two pcr-based protocols for serogrouping. a panel of 134 isolates (22 clinical samples, 79 samples of beef cuts, and 33 samples from the beef processing environment) were subjected to conventional serology and identified as serotypes 1/2a (n = 12), 1/2b (n = 21), 1/2c (n = 71), and 4b (n = 30). isolates from clinical samples were predominantly serotype 4b, and the most prev ...	2016	26735041
safety of street-vended soy wara in nigeria.	soy wara is a common ready-to-eat food whose production and sale are currently unregulated. microbiological sampling indicated that 21% of the samples had standard plate counts exceeding 100,000 cfu/g, and 14% had staphylococcus aureus counts higher than 100,000 cfu/g. the occurrence of s. aureus at these levels can result in food poisoning. listeria monocytogenes was isolated in 14.4% of the samples, although the counts were generally low, typically <1,000 cfu/g. although counts of l. monocytog ...	2016	26735046
[quantitative detection on contamination of listeria monocytogenes isolated from retail ready-to-eat meats in beijing].	to survey the contamination of l. monocytogenes isolated from retail ready-to-eat meats in beijing.	2015	26738383
tracking foodborne pathogenic bacteria in raw and ready-to-eat food illegally sold at the eastern eu border.	food illegally brought into the european union, mainly in the personal luggage of travelers, represents a potential threat to consumers' health. the aim of this study was to investigate the presence of five pathogens in food brought into the european union by moldavian citizens as personal goods and illegally sold in romania in the vicinity of the border. the occurrence of staphylococcus aureus and listeria monocytogenes was 7.5% and 8%, while campylobacter spp., escherichia coli o157:h7, and sa ...	2016	26741503
expression of listeria monocytogenes key virulence genes during growth in liquid medium, on rocket and melon at 4, 10 and 30 °c.	the aim of the present study was to assess the expression of key virulence genes, during growth of a listeria monocytogenes isolate in liquid medium, on melon and rocket at different temperatures and time. for that purpose, bhi broth, rocket and melon were inoculated at 7.0-7.5 log cfu ml(-1) or g(-1)and stored at 4, 10 and 30 °c. sampling took place upon inoculation and after 0.5, 6 and 24 h of incubation. the rna was stabilized and the expression of hly, plca, plcb, sigb, inla, inlb, inlc, inl ...	2016	26742611
effect of sanitizer combined with steam heating on the inactivation of foodborne pathogens in a biofilm on stainless steel.	the combined effect of chemical sanitizers including sodium hypochlorite, hydrogen peroxide, iodophor, and benzalkonium chloride with steam heating on the inactivation of biofilms formed by escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes on stainless steel was investigated. six day old biofilms, comprised of a mixture of three strains each of three foodborne pathogens, were produced on stainless steel coupons at 25 °c and treated with each sanitizer alone (for 5, 15, ...	2016	26742615
survival mechanism of escherichia coli o157:h7 against combined treatment with acetic acid and sodium chloride.	the combination of salt and acid is commonly used in the production of many foods, including pickles and fermented foods. however, in our previous studies, the addition of salt significantly reduced the inhibitory effect of acetic acid on escherichia coli o157:h7 in laboratory media and pickled cucumbers. therefore, this study was conducted to determine the mechanism by which salt confers resistance against acetic acid in e. coli o157:h7. the addition of high concentrations (up to 9% or 15% [w/v ...	2016	26742620
use of a novel medium, the polymyxin ceftazidime oxford medium, for isolation of listeria monocytogenes from raw or non-pasteurized foods.	polymyxin ceftazidime oxford medium (pcom), a novel selective and differential plating medium for listeria monocytogenes was compared with modified oxford agar (mox) for efficacy to isolate l. monocytogenes and other listeria spp. naturally present in non-pasteurized mexican-style cheese (n = 50), non-pasteurized fresh squeezed orange juice (n = 50), raw beef chunks (n = 36), and fresh cabbage (n = 125). samples were collected from retail markets and farms in mexico and tested following the us d ...	2016	26742621
advanced molecular diagnostic techniques for detection of food-borne pathogens; current applications and future challenges.	the elimination of disease-causing microbes from the food supply is a primary goal and this review deals with the overall techniques availavle for detection of food-borne pathogens. now-a-days conventional methods are replaced by advanced methods like biosensors, nucleic acid-based tests (nat) and different pcr based techniques used in molecular biology to identify specific pathogens. bacillus cereus, staphylococcus aureus, proteus vulgaris, escherichia coli, campylobacter, listeria monocytogene ...	2016	26745757
a novel enterocin t1 with anti-pseudomonas activity produced by enterococcus faecium t1 from chinese tibet cheese.	an enterocin-producing enterococcus faecium t1 was isolated from chinese tibet cheese. the enterocin was purified by sp-sepharose and reversed phase hplc. it was identified as unique from other reported bacteriocins based on molecular weight (4629 da) and amino acid compositions; therefore it was subsequently named enterocin t1. enterocin t1 was stable at 80-100 °c and over a wide ph range, ph 3.0-10.0. protease sensitivity was observed to trypsin, pepsin, papain, proteinase k, and pronase e. im ...	2016	26745981
effect of hops beta acids on the survival of unstressed- or acid-stress-adapted-listeria monocytogenes and on the quality and sensory attributes of commercially cured ham slices.	this study evaluated the antilisterial activity of hops beta acids (hba) and their impact on the quality and sensory attributes of ham. commercially cured ham slices were inoculated with unstressed- and acid-stress-adapted (asa)-l. monocytogenes (2.2 to 2.5 log cfu/cm(2) ), followed by no dipping (control), dipping in deionized (di) water, or dipping in a 0.11% hba solution. this was followed by vacuum or aerobic packaging and storage (7.2 °c, 35 or 20 d). samples were taken periodically during ...	2016	26748557
antimicrobial activity of neo-clerodane diterpenoids isolated from lamiaceae species against pathogenic and food spoilage microorganisms.	antimicrobial activity of nineteen neo-clerodane diterpenoids, isolated from the acetone extracts of the aerial parts of scutellaria and salvia species (lamiaceae) were tested against thirteen strains belonging to nine different species of pathogenic and food spoilage bacteria aeromonas hydrophila, bacillus cereus, escherichia coli, listeria monocytogenes, proteus vulgaris, pseudomonas aeruginosa, pseudomonas fluorescens, salmonella abony and staphylococcus aureus as well as against two yeast st ...	2015	26749799
zoonotic public health hazards in backyard chickens.	backyard poultry has become increasingly popular in industrialized countries. in addition to keeping chickens for eggs and meat, owners often treat the birds as pets. however, several pathogenic enteric bacteria have the potential for zoonotic transmission from poultry to humans but very little is known about the occurrence of zoonotic pathogens in backyard flocks. the occurrence and the antimicrobial resistance of salmonella enterica, campylobacter spp., listeria monocytogenes and enteropathoge ...	2016	26752227
listeria monocytogenes infection in ruminants: is there a link to the environment, food and human health? a review.	listeria (l.) monocytogenes is widely distributed in the environment, but also has the ability to cause serious invasive disease in ruminants and humans. this review provides an overview of listeriosis in ruminants and discusses our insufficient understanding of reservoirs and possible cycling ofl. monocytogenes between animal and human hosts, food and the environment. it indicates gaps in our knowledge of the role of genetic subtypes in l. monocytogenes ecology and virulence as well as risk fac ...	2015	26753347
gastric fluid and heat stress response of listeria monocytogenes inoculated on frankfurters formulated with 10%, 20%, and 30% fat content.	this study evaluated the effects of frankfurter fat content on listeria monocytogenes resistance to heat stress and gastric fluid, and the caco-2 cell invasion efficiency of the pathogen. a 10-strain mixture of l. monocytogenes was inoculated on frankfurters formulated with 10%, 20%, and 30% fat content (10%: f10, 20%: f20, 30%: f30) and stored at 10℃ for 30 d. the samples were analyzed for l. monocytogenes resistance to heat stress and a simulated gastric fluid challenge. the total bacteria and ...	2014	26760741
determination of shelf life for butter and cheese products in actual and accelerated conditions.	the aim of this study was to estimate the shelf life of butter and cheese products, with shelf life being a guide used to determine the storage period of food before deterioration. butter and cheese samples stored at 10℃ and 15℃ had a shelf life of 221 d, while those stored at 25℃ and 35℃ had a shelf life of 109 d. quality changes, including total cell count, coliform counts, listeria monocytogenes counts, acid value, moisture content, ph, acidity and overall sensory evaluation, were monitored. ...	2014	26760945
screening of bacteriocin-producing enterococcus faecalis strains for antagonistic activities against clostridium perfringens.	this study was conducted to isolate and characterize bacteriocin-producing bacteria against clostridium perfringens (c. perfringens) from domestic animals to determine their usefulness as probiotics. bacteriocin-producing bacteria were isolated from pig feces by the spot-on-lawn method. a total of 1,370 bacterial stains were isolated, and six were tentatively selected after identifying the inhibitory activity against the pathogenic indicator c. perfringens kctc 3269 and kctc 5100. the selected s ...	2014	26761495
comparison of culture, conventional and real-time pcr methods for listeria monocytogenes in foods.	we compared standard culture methods as well as conventional pcr and real-time pcr for the detection of listeria monocytogenes (l. monocytogenes) in milk, cheese, fresh-cut vegetables, and raw beef that have different levels of background microflora. no statistical differences were observed in sensitivity between the two selective media in all foods. in total, real-time pcr assay exhibited statistically excellent detection sensitivity (p<0.05) and was less time consuming and laborious as compare ...	2014	26761501
simultaneous detection of listeria monocytogenes, escherichia coli o157:h7, bacillus cereus, salmonella spp., and staphylococcus aureus in low-fatted milk by multiplex pcr.	a rapid and specific pcr assay for the simultaneous detection of listeria monocytogenes, escherichia coli o157:h7, bacillus cereus, salmonella spp., and staphylococcus aureus in foods was developed to reduce the detection time and to increase sensitivity. multiplex pcr developed in this study produced only acta, flic, hbl, inva, iles amplicons, but did not produce any non-specific amplicon. the primer sets successfully amplified the target genes in the multiplex pcr without any non-specific or a ...	2014	26761507
growth modelling of listeria monocytogenes in korean pork bulgogi stored at isothermal conditions.	the purpose of this study was to develop predictive models for the growth of listeria monocytogenes in pork bulgogi at various storage temperatures. a two-strain mixture of l. monocytogenes (atcc 15313 and isolated from pork bulgogi) was inoculated on pork bulgogi at 3 log cfu/g. l. monocytogenes strains were enumerated using general plating method on listeria selective medium. the inoculated samples were stored at 5, 15, and 25℃ for primary models. primary models were developed using the barany ...	2015	26761807
antilisterial bacteriocin from lactobacillus rhamnosus cjnu 0519 presenting a narrow antimicrobial spectrum.	a lactic acid bacterium presenting antimicrobial activity against a lactobacillus acidophilus strain used for eradication of acid inhibition was isolated from a natural cheese. the 16s rrna gene sequence of the isolate best matched with a strain of l. rhamnosus and was designated l. rhamnosus cjnu 0519. the antimicrobial activity of the partially purified bacteriocin of cjnu 0519 was abolished when treated with a protease, indicating the protein nature of the bacteriocin. the partially purified ...	2015	26761811
survey of yogurt powder storage in ambient export countries a safety evaluation standard compliance and comparative analysis.	yogurt powder is fermented milk processed in the form of dry yogurt, and has advantages such as stability, storability, convenience, and portability. china and vietnam are important export target countries because of the increased demand for dairy products. therefore, we surveyed dairy product standardization in order to establish an export strategy. lactic acid bacteria counts are unregulated in korea and vietnam. in china, lactic acid bacteria counts are regulated at 1×10(6) colony-forming uni ...	2015	26761812
antilisterial effect of bacteriocin sh01, obtained from enterococcus faecium sh01, in ground beef.	from the previous study, enterococcus faecium sh01 was isolated from mukeunji, an over-ripened kimchi, and it produced bacteriocin sh01. bacteriocin sh01 showed an inhibitory effect against listeria monocytogenes atcc 19111, a bacterial strain causing human listeriosis. crude bacteriocin sh01 was purified by ammonium sulfate precipitation and its inhibitory activity at two concentrations (500 and 1,000 au/g) against listeria monocytogenes atcc 19111 was investigated in ground beef at increasing ...	2015	26761830
combined effect of irradiation and ageing condition on physicochemical and microbial quality of hanwoo eye of round.	the combined effects of electron-beam irradiation and ageing of beef were examined. the irradiated samples at dose of 0 or 2 kgy were kept and analyzed for the microbial growth, shear values, meat color, and nucleotide-related flavor compounds at different ageing temperatures (2, 10, or 25℃) for 8 d. the irradiation effect on inactivation of foodborne pathogens was also investigated. the population of listeria monocytogenes and e. coli o157:h7 inoculated in beef samples decreased in proportion t ...	2015	26761855
occurrence and antibiotic susceptibility of listeria species in turkey meats.	the aims of this study were to investigate the occurrence of listeria species in turkey meats and to check the antimicrobial susceptibility of the isolated strains. hundred and fifteen raw turkey meat samples were randomly collected from the supermarkets, butchers and restaurants. strain isolation and identification were made according to the iso11290-1 method. antimicrobial susceptibility was determined by the standard disc diffusion method. a total of 47 listeria spp. were isolated from 115 (4 ...	2015	26761896
genome sequence of listeria monocytogenes strain f6540 (sequence type 360) collected from food samples in ontario, canada.	comparative genomic analysis between pathogenic and nonpathogenic listeria monocytogenes strains provides a good model for studying the virulence of this organism. here, we report the genome sequence of the nonpathogenic l. monocytogenes strain f6540 (sequence type 360) identified specifically in food samples in ontario, canada, in 2010.	2016	26769922
splenic leukocytes traffic to the thyroid and produce a novel tshβ isoform during acute listeria monocytogenes infection in mice.	the thyroid stimulating hormone beta-subunit (tshβ) with tshα form a glycoprotein hormone that is produced by the anterior pituitary in the hypothalamus-pituitary-thyroid (hpt) axis. although tshβ has been known for many years to be made by cells of the immune system, the role of immune system tsh has remained unclear. recent studies demonstrated that cells of the immune system produce a novel splice variant isoform of tshβ (tshβv), but little if any native tshβ. here, we show that within three ...	2016	26771831
reductions in neonatal listeriosis: "collateral benefit" of group b streptococcal prophylaxis?	we tested the hypothesis that declines in listeria monocytogenes in infants are related to declines in rates of early-onset group b streptococcus (gbs) infections associated with widespread prophylaxis using agents with listeria activity.	2016	26772166
impact of pathogen population heterogeneity and stress-resistant variants on food safety.	this review elucidates the state-of-the-art knowledge about pathogen population heterogeneity and describes the genotypic and phenotypic analyses of persister subpopulations and stress-resistant variants. the molecular mechanisms underlying the generation of persister phenotypes and genetic variants are identified. zooming in on listeria monocytogenes, a comparative whole-genome sequence analysis of wild types and variants that enabled the identification of mutations in variants obtained after a ...	2016	26772414
too much of a good thing: regulated depletion of c-di-amp in the bacterial cytoplasm.	bacteria that synthesize c-di-amp also encode several mechanisms for controlling c-di-amp levels within the cytoplasm. one major class of phosphodiesterases comprises gdpp and dhhp homologs, which degrade c-di-amp into the linear molecule 5'-papa or amp by the dhh-dhha1 domain. the other major class comprises pgph homologs, which degrade c-di-amp by the hd domain. both gdpp and pgph harbor sensory domains, likely to regulate c-di-amp hydrolysis activity in response to signal input. as another po ...	2016	26773214
effectiveness of superheated steam for inactivation of escherichia coli o157:h7, salmonella typhimurium, salmonella enteritidis phage type 30, and listeria monocytogenes on almonds and pistachios.	this study was undertaken to evaluate the effectiveness of superheated steam (shs) on the inactivation of escherichia coli o157:h7, salmonella typhimurium, salmonella enteritidis phage type (pt) 30 and listeria monocytogenes on almonds and in-shell pistachios and to determine the effect of superheated steam heating on quality by measuring color and texture changes. almonds and in-shell pistachios inoculated with four foodborne pathogens were treated with saturated steam (ss) at 100 °c and shs at ...	2016	26773253
short communication: inhibitory activities of the lantibiotic nisin combined with phenolic compounds against staphylococcus aureus and listeria monocytogenes in cow milk.	we aimed to investigate the antibacterial activities of carvacrol, thymol, eugenol, cinnamaldehyde, and lantibiotic nisin against standard bacterial strains of the milk pathogens staphylococcus aureus atcc 25923 and listeria monocytogenes atcc 15313 in cow milk. the minimum inhibitory concentrations (mic) of these substances were recorded. the synergistic effects were also assessed in culture medium (time kill curve) and in a food model (cow milk) during the storage period (4 °c for 6 d) after i ...	2016	26774728
antimicrobial effects of marine algal extracts and cyanobacterial pure compounds against five foodborne pathogens.	the marine environment is a proven source of structurally complex and biologically active compounds. in this study, the antimicrobial effects of a small collection of marine-derived extracts and isolates, were evaluated against 5 foodborne pathogens using a broth dilution assay. results demonstrated that algal extracts from padina and ulva species and cyanobacterial compounds antillatoxin b, laxaphycins a, b and b3, isomalyngamide a, and malyngamides c, i and j showed antimicrobial activity agai ...	2016	26775951
antilisterial activity on poultry meat of amylolysin, a bacteriocin from bacillus amyloliquefaciens ga1.	this paper describes the production, the purification and the antilisterial activity of amylolysin, a novel bacteriocin from b. amyloliquefaciens ga1. the strain genome was first analysed using pcr techniques for the presence of gene clusters that direct the synthesis of characterised bacteriocins from b. amyloliquefaciens and the closely related b. subtilis. our results suggest that amylolysin corresponds to a novel bacteriocin. the effect of amylolysin on the growth of different isolates of li ...	2010	26781121
evidence of antibacterial activities in peptide fractions originating from snow crab (chionoecetes opilio) by-products.	antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against gram-negative and gram-positive bacteria. among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the gram-negative bacteria such as aeromonas caviae, aeromonas hydrophila, campylobacter jejuni, listonella anguillarum, morganella morganii, shewanella putrefasciens, vibrio parahaemolyticus and vibrio vulnificus and against a few gram-posit ...	2010	26781242
investigation into the potential of bacteriocinogenic lactobacillus plantarum bfe 5092 for biopreservation of raw turkey meat.	the bacteriocin-producing lactobacillus plantarum bfe 5092 was assessed for its potential as a protective culture in the biopreservation of aerobically stored turkey meat. this strain produces three bacteriocins, i.e. plantaricins ef, jk and n. the absolute expression of lactobacillus plantarum bfe 5092 16s rrna housekeeping gene, as well as l-ldh, plnef and plng genes as determined by quantitative, real-time-pcr, revealed that these genes were expressed to similar levels when the strain was gro ...	2010	26781319
antibacterial activity of subtilosin alone and combined with curcumin, poly-lysine and zinc lactate against listeria monocytogenes strains.	in this paper, the antibacterial effects of the bacillus amyloliquefaciens-produced bacteriocin subtilosin, both alone and in combination with curcumin, ε-poly-l-lysine (poly-lysine), or zinc lactate, were examined against listeria monocytogenes. results indicated that subtilosin inhibits both of the studied bacterial strains, scott a (wild-type, nisin sensitive) and nr30 (nisin resistant). however, l. monocytogenes scott a was more sensitive to subtilosin and pure curcumin. in addition, subtilo ...	2010	26781320
biopreservation of sardinella longiceps and penaeus monodon using protective culture streptococcus phocae pi 80 isolated from marine shrimp penaeus indicus.	the influence of streptococcus phocae pi80 on the shelf life of sardinella longiceps and penaeus monodon was investigated by measurement of microbial and chemical analysis after appraising the safety of the protective probiotic culture in wistar rat's model. the results of this safety assessment indicate that oral administration of protective culture does not demonstrate any toxicological effects. consumption of this lab strain had no adverse effects on animal's general health status, hematology ...	2011	26781574
development of a murine model with optimal routes for bacterial infection and treatment, as determined with bioluminescent imaging in c57bl/6 mice.	mice intragastrically infected with listeria monocytogenes egde and staphylococcus aureus xen 36 showed no visible signs of infection over 48 h. however, high numbers (6.2 × 10(5) cfu/mg feces) of s. aureus xen 36 were detected 4 h, and 3.3 × 10(5) cfu/mg feces of l. monocytogenes egde 8 h, after administration. mice intraperitoneally infected with s. aureus xen 36 (1 × 10(7) cfu) developed infection immediately after administration and for at least the following 48 h. injection with higher cell ...	2011	26781578
recombinant expression of a putative amidase cloned from the genome of listeria monocytogenes that lyses the bacterium and its monolayer in conjunction with a protease.	listeria monocytogenes is a gram-positive, non-spore forming, catalase-positive rod that is a major bacterial food-borne disease agent associated with uncooked meats, including poultry, uncooked vegetables, soft cheeses, and unpasteurized milk. the bacterium may be carried by animals without signs of disease, can replicate at refrigeration temperatures, and is frequently associated with biofilms. there is a need to discover innovative pathogen intervention technologies for this bacterium. conseq ...	2012	26781731
mechanism of nisin, pediocin 34, and enterocin fh99 resistance in listeria monocytogenes.	nisin-, pediocin 34-, and enterocin fh99-resistant variants of listeria monocytogenes atcc 53135 were developed. in an attempt to clarify the possible mechanisms underlying bacteriocin resistance in l. monocytogenes atcc 53135, sensitivity of the resistant strains of l. monocytogenes atcc 53135 to nisin, pediocin 34, and enterocin fh99 in the absence and presence of different divalent cations was assessed, and the results showed that the addition of divalent cations significantly reduced the inh ...	2012	26781732
development of freeze-dried bacteriocin-containing preparations from lactic acid bacteria to inhibit listeria monocytogenes and staphylococcus aureus.	there has been a recent movement to produce and consume "minimally processed" and more "natural" foods through the use of fewer chemical preservatives. the shift to more "natural" foods has resulted in a great interest in the use of bacteriocins from lactic acid bacteria as natural biopreservatives. the objective of this comparative study was to identify bacteriocins that can be produced in low-cost or no-cost dairy-based media (dbm), concentrated using freeze-drying, and applied to cheddar chee ...	2012	26781734
comparison of the potency of the lipid ii targeting antimicrobials nisin, lacticin 3147 and vancomycin against gram-positive bacteria.	while nisin (lantibiotic), lacticin 3147 (lantibiotic) and vancomycin (glycopeptides) are among the best studied lipid ii-binding antimicrobials, their relative activities have never been compared. nisin and lacticin 3147 have been employed/investigated primarily as food preservatives, although they do have potential in terms of veterinary and clinical applications. vancomycin is used exclusively in clinical therapy. we reveal a higher potency for lacticin 3147 (mic 0.95-3.8 μg/ml) and vancomyci ...	2012	26781852
fluorescence-based comparative evaluation of bactericidal potency and food application potential of anti-listerial bacteriocin produced by lactic acid bacteria isolated from indigenous samples.	the aim of the present study was to ascertain the potency of anti-listerial bacteriocin produced by lactic acid bacteria (lab) isolated from indigenous samples of dahi, dried fish, and salt-fermented cucumber. a total of 231 lab isolates were obtained from the samples, of which 51 isolates displayed anti-listerial activity. the anti-listerial lab were identified by pcr as lactobacillus sp., pediococcus sp., enterococcus sp., and lactococcus sp. pcr also enabled the detection of class iia bacteri ...	2012	26781854
characterization of a reuterin-producing lactobacillus reuteri bpl-36 strain isolated from human infant fecal sample.	a reuterin (3-hydroxypropinaldehyde, 3-hpa)-producing isolate from a human infant fecal sample was identified as lactobacillus reuteri bpl-36 strain. the organism displayed a broad-spectrum antimicrobial activity. the gene (gdh) encoding a glycerol dehydratase subunit was detected by pcr, thus confirming its reuterin-producing ability. reuterin concentration of 89.63 mm/ml was obtained in the mrs-glycerol medium after 16 h of incubation at 37 °c. the reuterin concentration required to inhibit th ...	2012	26782041
stability and inhibitory activity of pediocin pa-1 against listeria sp. in simulated physiological conditions of the human terminal ileum.	listeria monocytogenes is responsible for severe foodborne infections, which can be life-threatening especially for infants and elderly populations. the emergence of antibiotic-resistant pathogens has stimulated the search for new strategies, such as the use of bacteriocins, to prevent or cure foodborne infectious diseases in the intestine. in this study, we evaluated the efficacy of the bacteriocin pediocin pa-1 from pediococcus acidilactici ul5 to inhibit listeria ivanovii, used as a surrogate ...	2012	26782185
antibacterial peptides, probiotic properties and biopreservative efficacy of native bacillus species isolated from different food sources.	in this study, we evaluated the occurrence of antibacterial peptide (abp)-producing bacillus spp. in fermented foods. among 78 isolated cultures, 25 potential abp-producing stains were selected and differentiated genotypically and phenotypically. the 16s rrna gene sequence homology, in combination with morphological, physiological and biochemical characteristics, was used for the identification of the isolates. the isolates exhibited inhibitory activity against both gram-positive and gram-negati ...	2012	26782188
prospective uses of recombinant lactococcus lactis expressing both listeriolysin o and mutated internalin a from listeria monocytogenes as a tool for dna vaccination.	in this study, lactococcus lactis was engineered to express mutated internalin a on its surface and to secrete large amounts of listeriolysin o (llo) in order to improve its potential as a vehicle for dna vaccination. western blotting experiments demonstrated that the bacterium expressed llo in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. a hemolytic assay showed that the recombinant strain secreted 250 ng active llo/mg total prot ...	2015	26782496
identification and partial characterization of antilisterial compounds produced by dairy yeasts.	food-grade yeasts make significant contributions to flavor development in fermented foods. some yeast species also inhibit undesirable bacteria, yeasts and molds, apparently by producing antimicrobial compounds called mycocins. the aim of this study was to evaluate the ability of wild yeasts, isolated from raw milk and cheese in the quebec province area, to produce antilisterial compounds. based on an agar-membrane screening test, 22 of 95 isolates, namely one candida catenulata, one candida par ...	2013	26782600
characterization and antilisterial effect of phosphatidylcholine nanovesicles containing the antimicrobial peptide pediocin.	encapsulation may provide increased stability and antimicrobial efficiency to bacteriocins. in this work, the antilisterial peptide pediocin was encapsulated in nanovesicles prepared from partially purified soybean phosphatidylcholine. the maintenance of antimicrobial activity and properties of free and encapsulated pediocin was observed during 13 days at 4 °c, and after this period, the encapsulated pediocin retained 50 % its initial activity. the maintenance of the bioactive properties of free ...	2013	26782604
development of a multi-pathogen enrichment broth for simultaneous growth of five common foodborne pathogens.	the objective of the present study was to formulate a multi-pathogen enrichment broth which could support the simultaneous growth of five common foodborne pathogens (salmonella enterica, staphylococcus aureus, shigella flexneri, listeria monocytogenes and escherichia coli o157:h7). the formulated broth sssle was composed of potassium tellurite, bile salt, lithium chloride, and sodium chloride as growth-inhibitors; glucose, esculin, mannitol and sodium pyruvate as growth-promoters. compared with ...	2015	26782652
partial characterization of bacteriocins produced by two new enterococcus faecium isolated from human intestine.	this study aimed at characterizing two novel bacteriocin-producing enterococcal strains isolated from human intestine. a total of 200 lactic acid bacteria were isolated from a woman stool sample. two of them were selected for characterization due to their high antimicrobial activity against five strains of listeria monocytogenes. the selected bacteria were identified as two different strains of enterococcus faecium and designated mt 104 and mt 162. the bacteriocins produced by mt 104 and mt 162 ...	2013	26782736
effect of bacteriocins and conditions that mimic food and digestive tract on biofilm formation, in vitro invasion of eukaryotic cells and internalin gene expression by listeria monocytogenes.	in this report, listeria monocytogenes isolates were evaluated for their ability to form biofilms, for adhesion/invasion of eukaryotic cells and for differential expression of internalin a (inl a) gene, which is related to virulence potential. the presence of bacteriocins of lactic acid bacteria and incubation at 5 °c were the main factors that influenced biofilm formation by l. monocytogenes, in comparison with bhi (control). in general, adhesion and invasion of caco-2 cells were significantly ...	2013	26782984
extent and mode of action of cationic legume proteins against listeria monocytogenes and salmonella enteritidis.	the methylated soybean protein and methylated chickpea protein (msp and mcp) with isoelectric points around pi 8 were prepared by esterifying 83 % of their free carboxyl groups and tested for their interactions with listeria monocytogenes and salmonella enteritidis. the two substances exhibited a concentration-dependent inhibitory action against the two studied bacteria with a minimum inhibitory concentration of about 100 μg/ml. the ic50 % of the two proteins against l. monocytogenes (17 μg/ml) ...	2013	26782988
anti-listerial bactericidal activity of lactobacillus plantarum dm5 isolated from fermented beverage marcha.	the strain lactobacillus plantarum dm5 was isolated from fermented beverage marcha of sikkim and explored for its antagonistic activity against food-borne pathogens. the cell-free supernatant of l. plantarum dm5 showed antibacterial activity of 6,400 au/ml in mrs medium (ph 6.0) against the indicator strain staphylococcus aureus. mrs medium supplemented with 15 g/l of maltose at 37 °c under static condition yielded highest antimicrobial activity (6,400 au/ml) with 3 % increase in specific activi ...	2013	26782989
the acid tolerance response alters membrane fluidity and induces nisin resistance in listeria monocytogenes.	the ability of l. monocytogenes cells to adapt to a variety of stressors contributes to its growth in a wide range of foods. the present study examines the effect of acid and of the acid tolerance response (atr) on membrane fluidity and on the organism's resistance to acid and to the bacteriocin nisin. when atr was induced in wild-type cells, these cells also became resistant to nisin. atr(+) cells also had lower membrane rigidities than control atr(-) cells that had not been subjected to the ac ...	2009	26783167
suppression of listeria monocytogenes scott a in fluid milk by free and liposome-entrapped nisin.	nisin is an antimicrobial polypeptide inhibitory toward gram-positive bacterial pathogens, including listeria monocytogenes. encapsulating nisin in lipid nanocapsules (i.e., liposomes) has been shown to protect antimicrobial functionality in complex food matrices. the capacity of liposomes to encapsulate a fluorescent reporter was determined via spectroscopy. survival and growth of l. monocytogenes incubated in fluid milk containing 50 iu/ml free or liposome-entrapped nisin was assayed via perio ...	2009	26783171
listeria monocytogenes' step-like response to sub-lethal concentrations of nisin.	microbial safety of food products is often accomplished by the formulation of food-grade preservatives into the product. because of the growing consumer demand for natural substances (including preservatives) in the composition of consumed foods, there is also a growing interest in the natural antimicrobial nisin, which has generally recognized as safe (gras) status for certain applications. during the products storage time, concentrations of preservative(s) are decreasing, which may eventually ...	2009	26783172
fate of mycobacterium tuberculosis in peroxidase-loaded resting murine macrophages.	myeloperoxidase (mpo), in the presence of hydrogen peroxide and a halide represent an efficient microbicidal mechanism of phagocytic cells. mpo is abundant in neutrophils which also respond to infection by producing large amounts of reactive oxygen species (ros). mpo, ros and halide constitute a very toxic antimicrobial system (called the klebanoff system or ks). resting mature macrophages do not contain granular mpo and thus are unable to kill pathogenic mycobacteria and some other microorganis ...	2013	26785781
efficacy of activated alginate-based nanocomposite films to control listeria monocytogenes and spoilage flora in rainbow trout slice.	essential oils of clove, coriander, caraway, marjoram, cinnamon, and cumin were tested for their antilisterial activity by application of agar diffusion assay (experiment 1). marjoram essential oil (meo) showed the highest inhibitory effect, followed by clove and cinnamon. subsequently, these essential oils were incorporated to alginate/clay nanocomposite films and antilisterial effectiveness of the films was studied in a model solid food system during 12 days at 10 °c (experiment 2). the result ...	2016	26787971
edible antimicrobial films based on microencapsulated lemongrass oil.	edible films and coatings have been proposed as viable alternatives for the preservation of fresh food such as fruit, meat, fish and cheese. they can be designed to contain natural antioxidants, vitamins and antimicrobials in order to extend shelf life of the product keeping the natural sensorial properties. essential oils have been targeted as potential active principles for edible films and coatings given their well-recognized antioxidant, antimicrobial and sensory properties. in the present w ...	2016	26788005
the incidence and distribution of listeria monocytogenes in ready-to-eat vegetables in south-western nigeria.	the study investigated the incidence of listeria monocytogenes in ready-to-eat (rte) vegetables: (cucumber, cucumis sativas; cabbage, brassica olerecea; carrot, daucus carota; tomato, solanum lycopersicum; lettuce, lactuca sativa) in six states in south-western nigeria. a total of 555 composite samples were collected from 30 traditional markets within the states and only 244 l. monocytogenes species were isolated by standard methods. the incidence of l. monocytogenes was low and not statisticall ...	2016	26788311
phenolic compounds, antioxidant, and antibacterial properties of pomace extracts from four virginia-grown grape varieties.	grape pomace is a potential source of natural antioxidant and antimicrobial agents. phenolic compounds, antioxidant, and antibacterial properties of pomace extracts from four virginia-grown grape varieties were investigated. white grape pomaces had higher (p < 0.05) solvent extraction yield than red varieties. concentrations of total phenolic (tpc), total flavonoid (tfc), total anthocyanin (tac), tannins, condensed tannins (ct), as well as antioxidant capacities (dpph• and abts•+free radical sca ...	2016	26788319
draft genome sequence of a 94-year-old listeria monocytogenes isolate, slcc208.	we report here the draft genome sequence of listeria monocytogenes strain slcc208 from seeliger's historical special listeria culture collection, initially cultured from a human case in france in 1921. this is, to our knowledge, the oldest l. monocytogenes isolate available and may be useful for comparative genomic studies of l. monocytogenes.	2016	26798096
acute conditions caused by infectious aortitis.	infection of the aorta is rare but potentially very dangerous. under normal circumstances the aorta is very resistant to infections. following some afflictions, the infection can pass to the aorta from blood or the surrounding tissues. the authors present their 5-year experience with therapy of various types of infections of the abdominal aorta.	2014	26798723
community-acquired bacterial meningitis in adults with cancer or a history of cancer.	to study the incidence, clinical presentation, causative bacteria, and outcome of community-acquired bacterial meningitis in adults with cancer.	2016	26802096
characterization of antimicrobial lipopeptides produced by bacillus sp. lm7 isolated from chungkookjang, a korean traditional fermented soybean food.	a wild-type microorganism exhibiting antimicrobial activities was isolated from the korean traditional fermented soybean food chungkookjang and identified as bacillus sp. lm7. during its stationary growth phase, the microorganism secreted an antimicrobial substance, which we partially purified using a simple two-step procedure involving ammonium sulfate precipitation and heat treatment. the partially purified antimicrobial substance, anti-lm7, was stable over a broad ph range (4.0-9.0) and at te ...	2016	26803269
the response of foodborne pathogens to osmotic and desiccation stresses in the food chain.	in combination with other strategies, hyperosmolarity and desiccation are frequently used by the food processing industry as a means to prevent bacterial proliferation, and particularly that of foodborne pathogens, in food products. however, it is increasingly observed that bacteria, including human pathogens, encode mechanisms to survive and withstand these stresses. this review provides an overview of the mechanisms employed by salmonella spp., shiga toxin producing e. coli, cronobacter spp., ...	2016	26803272
effect of phytosanitary irradiation and methyl bromide fumigation on the physical, sensory, and microbiological quality of blueberries and sweet cherries.	the objective of this study was to determine whether irradiation could serve as a suitable phytosanitary treatment alternative to methyl bromide (mb) fumigation for blueberries and sweet cherry and also to determine the effect of phytosanitary irradiation treatment on survival of salmonella spp. and listeria monocytogenes on these fruit. 'bluecrop' blueberries (vaccinium corymbosum) and 'sweetheart' cherries (prunus avium) were irradiated at 0.4 kgy or fumigated with methyl bromide and evaluated ...	2016	26805024
evaluation of probiotic properties of lactobacillus plantarum wlpl04 isolated from human breast milk.	lactobacillus plantarum wlpl04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on caco-2 cells). the results showed that lb. plantarum wlpl04 had broad-spec ...	2016	26805974
[cabozantinib inhibits listeria monocytogenes infection in mice].	to test the effect of the c-met inhibitor cabozantinib in inhibiting infections by listeria monocytogenes (lm) in mice.	2016	26806739
invasiveness of listeria monocytogenes strains isolated from animals in poland.	animals are important reservoir of listeria monocytogenes, a pathogen causing serious infections in both humans and livestock. however, data on invasiveness of l. monocytogenes strains of animal origin is very scarce. ability of 18 l. monocytogenes strains of animal origin to invade ht-29 cells was investigated. plaque forming assay was used to assess invasiveness and ability of the pathogen to spread in the cell line. almost 40% of l. monocytogenes strains were weakly invasive. it was shown tha ...	2015	26812809
prosthetic knee joint infection due to listeria monocytogenes bacteremia in a diabetic female.		2016	26813571
modeling the impact of ingoing sodium nitrite, sodium ascorbate, and residual nitrite concentrations on growth parameters of listeria monocytogenes in cooked, cured pork sausage.	sodium nitrite has been identified as a key antimicrobial ingredient to control pathogens in ready-to-eat (rte) meat and poultry products, including listeria monocytogenes. this study was designed to more clearly elucidate the relationship between chemical factors (ingoing nitrite, ascorbate, and residual nitrite) and l. monocytogenes growth in rte meats. treatments of cooked, cured pork sausage (65% moisture, 1.8% salt, ph 6.6, and water activity 0.98) were based on response surface methodology ...	2016	26818978
inactivation of escherichia coli, listeria monocytogenes, and salmonella enteritidis by cymbopogon citratus d.c. stapf. essential oil in pineapple juice.	in the present study, the efficacy of cymbopogon citratus d.c. stapf. essential oil (cceo) to provoke a 5-log cfu/ml (5-log) inactivation in a mixed composite of escherichia coli, listeria monocytogenes, and salmonella enterica serovar enteritidis in pineapple (ananas comosus (l.) merril) juice (4°c) was assessed. moreover, the effects of cceo on the physicochemical and sensory quality parameters of pineapple juice were evaluated. the mic of cceo was 5 μl/ml against the composite mix examined. f ...	2016	26818981
various ready-to-eat products from retail stores linked to occurrence of diverse listeria monocytogenes and listeria spp. isolates.	listeriosis outbreaks have been associated with a variety of foods. this study investigated the prevalence and diversity of listeria monocytogenes and listeria spp. in ready-to-eat (rte) products and evaluated the performance of a rapid detection method, the 3m molecular detection assay for l. monocytogenes (mda-lm), for detection of l. monocytogenes. assay results were compared with those obtained using the u.s. food and drug administration standard culture method described in the bacteriologic ...	2016	26818984
effects of the essential oil from origanum vulgare l. on survival of pathogenic bacteria and starter lactic acid bacteria in semihard cheese broth and slurry.	this study assessed the inhibitory effects of the essential oil from origanum vulgare l. (oveo) on staphylococcus aureus, listeria monocytogenes, and a mesophilic starter coculture composed of lactic acid bacteria (lactococcus lactis subsp. lactis and l. lactis subsp. cremoris) in brazilian coalho cheese systems. the mic of oveo was 2.5 μl/ml against both s. aureus and l. monocytogenes and 0.6 μl/ml against the tested starter coculture. in cheese broth containing oveo at 0.6 μl/ml, no decrease i ...	2016	26818985
antimicrobial activity of individual and combined essential oils against foodborne pathogenic bacteria.	the antimicrobial activities of essential oils from mexican oregano (lippia berlandieri schauer), mustard (brassica nigra), and thyme (thymus vulgaris) were evaluated alone and in binary combinations against listeria monocytogenes, staphylococcus aureus, or salmonella enteritidis. chemical compositions of the essential oils were analyzed by gas chromatography-mass spectrometry. the mics of the evaluated essential oils ranged from 0.05 to 0.50% (vol/vol). mustard essential oil was the most effect ...	2016	26818994
fresh produce-associated listeriosis outbreaks, sources of concern, teachable moments, and insights.	foodborne transmission of listeria monocytogenes was first demonstrated through the investigation of the 1981 maritime provinces outbreak involving coleslaw. in the following two decades, most listeriosis outbreaks involved foods of animal origin, e.g., deli meats, hot dogs, and soft cheeses. l. monocytogenes serotype 4b, especially epidemic clones i, ii, and ia, were frequently implicated in these outbreaks. however, since 2008 several outbreaks have been linked to diverse types of fresh produc ...	2016	26818997
listeria meningoencephalitis and anti-gq1b antibody syndrome.	we report the first case of listeria monocytogenes meningoencephalitis associated with anti-gq1b antibody syndrome in an immunocompetent adult. a prompt diagnosis, made thanks to the multidisciplinary contribution, allowed a combined therapeutic approach leading to final favourable outcome, despite several intercurrent complications.	2016	26825308
inhibition of sortase a by chalcone prevents listeria monocytogenes infection.	the critical role of sortase a in gram-positive bacterial pathogenicity makes this protein a good potential target for antimicrobial therapy. in this study, we report for the first time the crystal structure of listeria monocytogenes sortase a and identify the active sites that mediate its transpeptidase activity. we also used a sortase a (srta) enzyme activity inhibition assay, simulation, and isothermal titration calorimetry analysis to discover that chalcone, an agent with little anti-l. mono ...	2016	26826492
listeria monocytogenes cholecystitis in an immunocompetent patient.		2017	26826779
uncovering listeria monocytogenes hypervirulence by harnessing its biodiversity.	microbial pathogenesis studies are typically performed with reference strains, thereby overlooking within-species heterogeneity in microbial virulence. here we integrated human epidemiological and clinical data with bacterial population genomics to harness the biodiversity of the model foodborne pathogen listeria monocytogenes and decipher the basis of its neural and placental tropisms. taking advantage of the clonal structure of this bacterial species, we identify clones epidemiologically assoc ...	2016	26829754
perforin-2 protects host cells and mice by restricting the vacuole to cytosol transitioning of a bacterial pathogen.	the host-encoded perforin-2 (encoded by the macrophage-expressed gene 1, mpeg1), which possesses a pore-forming macpf domain, reduces the viability of bacterial pathogens that reside within membrane-bound compartments. here, it is shown that perforin-2 also restricts the proliferation of the intracytosolic pathogen listeria monocytogenes within a few hours of systemic infection, the massive proliferation of l. monocytogenes in perforin-2(-/-)mice leads to a rapid appearance of acute disease symp ...	2016	26831467
validation of the ansr(®) listeria monocytogenes method for detection of listeria monocytogenes in selected food and environmental samples.	work was conducted to validate performance of the ansr(®) for listeria monocytogenes method in selected food and environmental matrixes. this dna-based assay involves amplification of nucleic acid via an isothermal reaction based on nicking enzyme amplification technology. following single-step sample enrichment for 16-24 h for most matrixes, the assay is completed in 40 min using only simple instrumentation. when 50 distinct strains of l. monocytogenes were tested for inclusivity, 48 produced p ...	2017	26833248
listeria monocytogenes σh contributes to expression of competence genes and intracellular growth.	the alternative sigma factor σ(h)has two functions in gram-positive bacteria: it regulates sporulation and the development of genetic competence. listeria monocytogenes is a nonsporulating species in which competence has not yet been detected. nevertheless, the main competence regulators and a series of orthologous genes that form the competence machinery are present in its genome; some of the competence genes play a role in optimal phagosomal escape. in this study, strains overexpressing σ(h) a ...	2016	26833412