Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| macrophage interaction with paracoccidioides brasiliensis yeast cells modulates fungal metabolism and generates a response to oxidative stress. | macrophages are key players during paracoccidioides brasiliensis infection. however, the relative contribution of the fungal response to counteracting macrophage activity remains poorly understood. in this work, we evaluated the p. brasiliensis proteomic response to macrophage internalization. a total of 308 differentially expressed proteins were detected in p. brasiliensis during infection. the positively regulated proteins included those involved in alternative carbon metabolism, such as enzym ... | 2015 | 26360774 |
| paracoccidioides brasiliensis induces recruitment of α3 and α5 integrins into epithelial cell membrane rafts, leading to cytokine secretion. | paracoccidioides brasiliensis is one of the etiological agents of paracoccidioidomycosis, a human systemic mycosis, highly prevalent in latin america. in the present work, we demonstrate that p. brasiliensis yeasts promote il-6 and il-8 secretion by the human lung epithelial cell line a549 in an integrin-dependent manner. in fact, small interfering rna directed to α3 and α5 integrins decreased il-6 and il-8 levels in p. brasiliensis-infected a549 cell cultures. this fungus also led to an increas ... | 2016 | 26369712 |
| distinct patterns of yeast cell morphology and host responses induced by representative strains of paracoccidioides brasiliensis (pb18) and paracoccidioides lutzii (pb01). | paracoccidioidomycosis (pcm) is a systemic mycosis, widespread in latin america. pcm is a granulomatous disease characterized by a polymorphism of lesions depending on the pathogen's virulence, the immune status of the host and its genetic susceptibility. the thermodimorphic fungus paracoccidioides brasiliensis was considered the only etiologic agent of pcm, yet recent works have shown significant genetic diversity among different strains of p. brasiliensis. therefore, it has been proposed for a ... | 2016 | 26384386 |
| extracellular vesicles from paracoccidioides pathogenic species transport polysaccharide and expose ligands for dc-sign receptors. | extracellular vesicles (evs) mediate non-conventional transport of molecules across the fungal cell wall. we aimed at describing the carbohydrate composition and surface carbohydrate epitopes of evs isolated from the pathogenic fungi paracoccidioides brasiliensis and p. lutzii using standard procedures. total ev carbohydrates were ethanol-precipitated from preparations depleted of lipids and proteins, then analyzed by chemical degradation, gas chromatography-mass spectrometry, nuclear magnetic r ... | 2015 | 26387503 |
| expression of dectin-1 and enhanced activation of nalp3 inflammasome are associated with resistance to paracoccidioidomycosis. | dectin-1 is a pattern recognition receptor (prr) that recognizes β-glucans and plays a major role in the immunity against fungal pathogens. paracoccidioides brasiliensis, the causative agent of paracoccidioidomycosis, has a sugar-rich cell wall mainly composed of mannans and glucans. to investigate the role of dectin-1 in the innate immunity of resistant (a/j) and susceptible (b10.a) mice to p. brasiliensis infection, we evaluated the role of curdlan (a dectin-1 agonist) and laminarin (a dectin- ... | 2015 | 26388856 |
| participation of dectin-1 receptor on nets release against paracoccidioides brasiliensis: role on extracellular killing. | paracoccidioides brasiliensis is a dimorphic fungus from the paracoccidioides genus, which is the causative agent of paracoccidioidomycosis, a chronic, subacute or acute mycosis, with visceral and cutaneous involvement. this disease that is acquired through inhalation primarily attacks the lungs but, can spread to other organs. phagocytic cells as neutrophils play an important role during innate immune response against this fungus, but studies on antifungal activities of these cells are scarce. ... | 2016 | 26416210 |
| the power of the small: the example of paracoccidioides brasiliensis conidia. | research on paracoccidioides brasiliensis has centered in the yeast cell probably because of the lack of distinctive features in the mycelium. in 1942 and for the first time, lateral conidia were noticed in the fungus' hyphae. later on, brazilian, venezuelan and argentinean researchers described "aleurias" when the fungus was grown in natural substrates. in 1970 authors became interested in the conidia and were able to obtain them in large numbers and treat them as individual units. their shape ... | 2015 | 26465363 |
| epidemiology of paracoccidioidomycosis. | the epidemiological characteristics of paracoccidioidomycosis were reviewed and updated. the new endemic areas in brazil were discussed in the section regarding the geographic distribution of the mycosis. subclinical infection with paracoccidioides brasiliensis was discussed on the basis of skin test surveys with antigens of the fungus, seroepidemiological studies, and disease cases outside latin america. large case series permitted a comparison of the prevalence of the mycosis in different regi ... | 2015 | 26465364 |
| paracoccidioides brasiliensis and paracoccidioides lutzii, a secret love affair. | to commemorate prof. carlos da silva lacaz's centennial anniversary, the authors have written a brief account of a few, out of hundreds, biological, ecological, molecular and phylogenetic studies that led to the arrival of paracoccidioides lutzii, hidden for more than a century within paracoccidioides brasiliensis. lacaz's permanent interest in this fungus, and particularly his conviction on the benefits that research on paracoccidioidomycosis would bring to patients, were pivotal in the develop ... | 2015 | 26465366 |
| advances and challenges in paracoccidioidomycosis serology caused by paracoccidioides species complex: an update. | understanding the possible methodologies for the rapid and inexpensive identification of fungal infections is essential for disease diagnosis, but there are some limitations. to help with this problem, serological methods that detect antigens or antibodies are widely used and are useful for the diagnosis of paracoccidioidomycosis (pcm) through the detection of gp43, which is the main antigen employed for the immunodiagnosis of this disease caused by paracoccidioides brasiliensis. however, the us ... | 2016 | 26494541 |
| data in support of quantitative proteomics to identify potential virulence regulators in paracoccidioides brasiliensis isolates. | paracoccidioides genus are the etiologic agents of paracoccidioidomycosis (pcm), a systemic mycosis endemic in latin america. few virulence factors have been identified in these fungi. this paper describes support data from the quantitative proteomics of paracoccidioides brasiliensis attenuated and virulent isolates [1]. the protein compositions of two isolates of the pb18 strain showing distinct infection profiles were quantitatively assessed by stable isotopic dimethyl labeling and proteomic a ... | 2015 | 26501084 |
| high interleukin-4 expression and interleukin-4 gene polymorphisms are associated with susceptibility to human paracoccidioidomycosis. | paracoccidioidomycosis (pcm) is caused by dimorphic fungi from the paracoccidioides brasiliensis complex. previous studies have demonstrated that the severity of disease is associated with a t-helper 2 immune response characterised by high interleukin (il)-4 production. in the present study we analysed two polymorphisms in the il-4 gene (-590 c/t and intron-3 microsatellite) in 76 patients with pcm and 73 control subjects from an endemic area. the production of il-4 by peripheral blood mononucle ... | 2015 | 26517657 |
| functional analysis of paracoccidioides brasiliensis 14-3-3 adhesin expressed in saccharomyces cerevisiae. | 14-3-3 proteins comprise a family of eukaryotic multifunctional proteins involved in several cellular processes. the pb14-3-3 of paracoccidioides brasiliensis seems to play an important role in the paracoccidioides-host interaction. paracoccidioides brasiliensis is an etiological agent of paracoccidioidomycosis, which is a systemic mycosis that is endemic in latin america. in the initial steps of the infection, paracoccidioides spp. synthetizes adhesins that allow it to adhere and invade host ce ... | 2015 | 26537993 |
| the effects of paracoccidioides brasiliensis infection on gm-csf- and m-csf-induced mouse bone marrow-derived macrophage from resistant and susceptible mice strains. | considering the importance of macrophages as the first line of defense against fungal infection and the different roles played by the two m1- and m2-like polarized macrophages, we decided to evaluate the effects of paracoccidioides brasiliensis infection on gm-csf- and m-csf-induced bone marrow-derived macrophages (bmm) from the a/j and b10.a mouse strains, an established model of resistance/susceptibility to pcm, respectively. upon differentiation, the generated gm- or m-bmms were characterized ... | 2015 | 26543326 |
| comparison of virulence between paracoccidioides brasiliensis and paracoccidioides lutzii using galleria mellonella as a host model. | paracoccidioidomycosis is a systemic mycosis, endemic in latin america. the etiologic agents of this mycosis are composed of 2 species: paracoccidioides brasiliensis and p. lutzii. murine animal models are the gold standard for in vivo studies; however, ethical, economical and logistical considerations limit their use. galleria mellonella is a suitable model for in vivo studies of fungal infections. in this study, we compared the virulence of p. brasiliensis and p. lutzii in g. mellonella model. ... | 2015 | 26552324 |
| mycologic endocrinology. | the interactions of fungi and chemical messenger molecules, hormones or pheromones, are addressed in this chapter. these interactions include mammalian fungal pathogens, also plant pathogens, or non-pathogenic fungi, which can result in functional responses in receptor- or non-receptor-mediated fashions. endogenous ligands in the fungi have been demonstrated to be important for mating in a number of systems. mammalian hormones have been demonstrated to have stimulatory or inhibitory effects on g ... | 2016 | 26589227 |
| tlr 9 involvement in early protection induced by immunization with rpb27 against paracoccidioidomycosis. | paracoccidioidomycosis is caused by fungi of the paracoccidioides genus and constitutes the most prevalent deep mycosis in latin america. toll-like receptors promote immune response against infectious agents. recently, it was reported that tlr9 is crucial for mice survival during the first 48 h of p. brasiliensis infection. in this study, we used cpg oligodeoxynucleotide motif as an adjuvant with and without rpb27 to immunize mice against paracoccidioidomycosis. cpg adjuvant induced differential ... | 2016 | 26597327 |
| serological investigation into paracoccidioides brasiliensis infection in dogs from southern rio grande do sul, brazil. | paracoccidioides brasiliensis is a dimorphic fungus and major cause of paracoccidioidomycosis (pcm), which is a systemic and endemic mycosis in brazil. in southern brazil, an increased number of cases were detected since the 1990 s. therefore, in order to determine areas with the presence of the fungus, this study aimed to investigate infection by p. brasiliensis in dogs from southern brazil. indirect elisa was used to detect antibodies against p. brasiliensis gp43. one hundred and ninety-six st ... | 2016 | 26608381 |
| lipoxin inhibits fungal uptake by macrophages and reduces the severity of acute pulmonary infection caused by paracoccidioides brasiliensis. | cysteinyl leukotrienes (cyslts) and lipoxins (lxs) are lipid mediators that control inflammation, with the former inducing and the latter inhibiting this process. because the role played by these mediators in paracoccidioidomycosis was not investigated, we aimed to characterize the role of cyslt in the pulmonary infection developed by resistant (a/j) and susceptible (b10.a) mice. 48 h after infection, elevated levels of pulmonary ltc4 and lxa4 were produced by both mouse strains, but higher leve ... | 2015 | 26635449 |
| paracoccidioides-host interaction: an overview on recent advances in the paracoccidioidomycosis. | paracoccidioides brasiliensis and p. lutzii are etiologic agents of paracoccidioidomycosis (pcm), an important endemic mycosis in latin america. during its evolution, these fungi have developed characteristics and mechanisms that allow their growth in adverse conditions within their host through which they efficiently cause disease. this process is multi-factorial and involves host-pathogen interactions (adaptation, adhesion, and invasion), as well as fungal virulence and host immune response. i ... | 2015 | 26635779 |
| decreased expression of 14-3-3 in paracoccidioides brasiliensis confirms its involvement in fungal pathogenesis. | the interaction between the fungal pathogen paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. one molecule implicated in the p. brasiliensis-host interaction is the 14-3-3 protein. the 14-3-3 protein belongs to a family of conserved regulatory molecules that are expressed in all eukaryotic cells and are involved in diverse cellular functions. here, we i ... | 2016 | 26646480 |
| new insights into a complex fungal pathogen: the case of paracoccidioides spp. | paracoccidioidomycosis is a systemic mycosis endemic to latin america, with paracoccidioides brasiliensis and p. lutzii being the causal agents of this disorder. several issues have been raised in the 100 years since its discovery and in this article we discuss features of this fascinating fungal pathogen, including its biology, eco-epidemiology and aspects of its pathogenicity. we also consider some of its virulence determinants, the most recent advances in the study of its metabolic pathways a ... | 2016 | 26683539 |
| lacaziosis-like disease in tursiops truncatus from brazil: a histopathological and immunohistochemical approach. | cetacean lacaziosis-like disease or lobomycosis-like disease (lld) is a chronic skin condition caused by a non-cultivable yeast of the order onygenales, which also includes lacazia loboi, as well as paracoccidioides brasiliensis and p. lutzii, respectively responsible for lacaziosis and paracoccidioidomycosis in humans. complete identification and phylogenetic classification of the lld etiological agent still needs to be elucidated, but preliminary phylogenetic analyses have shown a closer relat ... | 2016 | 26758656 |
| paracoccidioides brasiliensis interacts with dermal dendritic cells and keratinocytes in human skin and oral mucosa lesions. | paracoccidioidomycosis (pcm) is a systemic disease caused by the fungus paracoccidioides brasiliensis and paracoccidioides lutzii. in pcm the skin and oral mucosa are often affected. dendritic cells and keratinocytes of the integument play a role in innate and adaptive immune response against pathogens, due to their function as antigen presenting cells. aiming to verify the interaction of p. brasiliensis with these cell populations, we studied 52 skin and 47 oral mucosa samples taken from patien ... | 2016 | 26768374 |
| cokeromyces recurvatus in a cervical papanicolaou test: a case report of a rare fungus with a brief review of the literature. | here we report a rare case of cokeromyces recurvatus in a pap test in an asymptomatic pregnant patient. each liquid-based pap slide contained multiple rounded yeast-like forms measuring 10-30 µm including some profiles with peripheral small buds. the background had neither epithelial abnormality nor any significant inflammation. a positive silver stain aided in confirming a fungal organism. rare fungi were considered including paracoccidioides brasiliensis due to the 'mariner's wheel' morphology ... | 2016 | 26800441 |
| antibodies against glycolipids enhance antifungal activity of macrophages and reduce fungal burden after infection with paracoccidioides brasiliensis. | paracoccidioidomycosis is a fungal disease endemic in latin america. polyclonal antibodies to acidic glycosphingolipids (gsls) from paracoccidioides brasiliensis opsonized yeast forms in vitro increasing phagocytosis and reduced the fungal burden of infected animals. antibodies to gsl were active in both prophylactic and therapeutic protocols using a murine intratracheal infection model. pathological examination of the lungs of animals treated with antibodies to gsl showed well-organized granulo ... | 2016 | 26870028 |
| detection of multiple budding yeast cells and a partial sequence of 43-kda glycoprotein coding gene of paracoccidioides brasiliensis from a case of lacaziosis in a female pacific white-sided dolphin (lagenorhynchus obliquidens). | lacaziosis, formerly called as lobomycosis, is a zoonotic mycosis, caused by lacazia loboi, found in humans and dolphins, and is endemic in the countries on the atlantic ocean, indian ocean and pacific ocean of japanese coast. susceptible cetacean species include the bottlenose dolphin (tursiops truncatus), the indian ocean bottlenose dolphin (t. aduncus), and the estuarine dolphin (sotalia guianensis); however, no cases have been recorded in other cetacean species. we diagnosed a case of lacazi ... | 2016 | 26883513 |
| paracoccidioidomycosis in a renal transplant recipient. | paracoccidioidomycosis (pcm) is the most common endemic mycosis in latin america. the etiological agents, which comprise two species, paracoccidioides brasiliensis and p. lutzii, are thermodimorphic fungi that usually affect previously healthy adults. they primarily involve the lungs and then disseminate to other organs. such mycosis is rare in organ transplant recipients; there have been only three cases reported in literature, until now. we report a case of pcm in a renal transplant recipient ... | 2016 | 26910451 |
| thioridazine inhibits gene expression control of the cell wall signaling pathway (cwi) in the human pathogenic fungus paracoccidioides brasiliensis. | paracoccidioides brasiliensis is a thermodimorphic fungus associated with paracoccidioidomycosis (pcm), the most common systemic mycosis in latin america. pcm treatment involves a long-term chemotherapeutic approach and relapses occur at an alarming frequency. moreover, the emergence of strains with increased drug-resistance phenotypes puts constant pressure on the necessity to develop new alternatives to treat systemic mycoses. in this work, we show that the phenothiazine (ptz) derivative thior ... | 2016 | 26956010 |
| antifungal activity of extracts from atacama desert fungi against paracoccidioides brasiliensis and identification of aspergillus felis as a promising source of natural bioactive compounds. | fungi of the genus paracoccidioides are responsible for paracoccidioidomycosis. the occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. compounds extracted from fungal extract have showing antifungal activity. extracts of 78 fungi isolated from rocks of the atacama desert were tested in a microdilution assay against paracoccidioides brasiliensis pb18. approximately 18% (5) of the extracts showed minimum inhibitory concentration (mic) values ≤ ... | 2016 | 27008375 |
| environmental mapping of paracoccidioides spp. in brazil reveals new clues into genetic diversity, biogeography and wild host association. | paracoccidioides brasiliensis and paracoccidioides lutzii are the etiological agents of paracoccidioidomycosis (pcm), and are easily isolated from human patients. however, due to human migration and a long latency period, clinical isolates do not reflect the spatial distribution of these pathogens. molecular detection of p. brasiliensis and p. lutzii from soil, as well as their isolation from wild animals such as armadillos, are important for monitoring their environmental and geographical distr ... | 2016 | 27045486 |
| experimental paracoccidioidomycosis in pregnant rats. | paracoccidioidomycosis (pcm), caused by the dimorphic fungus paracoccidioides brasiliensis (pb), is the most prevalent systemic mycosis in latin america. there are few reports in the literature about the disease damages during pregnancy and the consequences to the fetuses and breeding. this study evaluated the implications of pcm during pregnancy on offspring and mothers in wistar rats. groups of rats were submitted to systemic pb infection, by intraperitoneal infusion, and mated 30 days after t ... | 2015 | 27049707 |
| acute paracoccidioidomycosis due to paracoccidioides brasiliensis s1 mimicking hypereosinophilic syndrome with massive splenomegaly: diagnostic challenge. | 2016 | 27054891 | |
| a few shared up-regulated genes may influence conidia to yeast transformation in dimorphic fungal pathogens. | the small number of fungi that commonly cause disease in normal people share the capacity to grow as mycelia in the soil at 25°c and as yeast (or spherules) in mammals at 37°c. this remarkable conversion has long been a topic of interest in medical mycology. the conidia to yeast conversion has been studied by transcription profiling in several fungal species, including histoplasma capsulatum, paracoccidioides brasiliensis, coccidioides spp., blastomyces dermatitidis, and talaromyces marneffei on ... | 2016 | 27118798 |
| paracoccidioides brasiliensis ps2: first autochthonous paracoccidioidomycosis case report in rio de janeiro, brazil, and literature review. | paracoccidioidomycosis (pcm) is a systemic mycosis caused by pathogenic dimorphic fungi of the paracoccidioides brasiliensis complex. it is the most important systemic mycosis in latin america, mainly in brazil. despite its severity and high mortality rates, it is considered a neglected disease. species within the genus paracoccidioides present genetics and morphological variations with probable clinical, diagnostic and therapeutic consequences. in fact, there are a very small number of detailed ... | 2016 | 27161127 |
| paracoccidioides brasiliensis infection promotes thymic disarrangement and premature egress of mature lymphocytes expressing prohibitive tcrs. | paracoccidioidomycosis, a chronic granulomatous fungal disease caused by paracoccidioides brasiliensis yeast cells affects mainly rural workers, albeit recently cases in immunosuppressed individuals has been reported. protective immune response against p. brasiliensis is dependent on the activity of helper t cells especially ifn-γ-producing th1 cells. it has been proposed that paracoccidioides brasiliensis is able to modulate the immune response towards a permissive state and that the thymus pla ... | 2016 | 27189089 |
| influence of n-glycans on expression of cell wall remodeling related genes in paracoccidioides brasiliensis yeast cells. | paracoccidioidomycosis is the most prevalent systemic mycosis in latin america. it is caused by the temperature-dependent dimorphic fungus paracoccidioides brasiliensis. the p. brasiliensis cell wall is a dynamic outer structure, composed of a network of glycoproteins and polysaccharides, such as chitin, glucan and n-glycosylated proteins. these glycoproteins can interact with the host to affect infection rates, and are known to perform other functions. we inhibited n-linked glycosylation using ... | 2016 | 27226767 |
| oral paracoccidioidomycosis granulomas are predominantly populated by cd163+ multinucleated giant cells. | multinucleated giant cells (mgc) are considered to be a hallmark of granulomatous inflammation; thus, they may play an essential role in the host response against pathogens, particularly paracoccidioides brasiliensis. this study characterizes the mgc found in oral paracoccidioidomycosis and assesses the correlation of mgc with the amount of fungi within oral tissues. twenty-six cases were included. they were classified as loose or dense granulomas, and the total mgc, including foreign-body and l ... | 2016 | 27236303 |
| musculoskeletal ultrasound findings in paracoccidioidomycosis. | paracoccidioidomycosis is a fungal infection endemic to south america. the infection is usually asymptomatic and mostly affects the upper and lower respiratory tracts with clinical-radiological dissociation. joint involvement is rare with no specific pattern or radiological injury. we report a case of paracoccidioidomycosis in which the patient's initial symptoms were hoarseness and arthritis. after an ultrasound examination, we performed the differential diagnosis of other noninfectious arthrop ... | 2016 | 27267337 |
| a conserved dimorphism-regulating histidine kinase controls the dimorphic switching in paracoccidioides brasiliensis. | paracoccidioides brasiliensis and p. lutzii, thermally dimorphic fungi, are the causative agents of paracoccidioidomycosis (pcm). paracoccidioides infection occurs when conidia or mycelium fragments are inhaled by the host, which causes the paracoccidioides cells to transition to the yeast form. the development of disease requires conidia inside the host alveoli to differentiate into yeast cells in a temperature-dependent manner. we describe the presence of a two-component signal transduction sy ... | 2016 | 27268997 |
| antifungal activity of amphotericin b conjugated to nanosized magnetite in the treatment of paracoccidioidomycosis. | this study reports on in vitro and in vivo tests that sought to assess the antifungal activity of a newly developed magnetic carrier system comprising amphotericin b loaded onto the surface of pre-coated (with a double-layer of lauric acid) magnetite nanoparticles. the in vitro tests compared two drugs; i.e., this newly developed form and free amphotericin b. we found that this nanocomplex exhibited antifungal activity without cytotoxicity to human urinary cells and with low cytotoxicity to peri ... | 2016 | 27303789 |
| paracoccidioidomycosis in the spine: case report and review of the literature. | paracoccidioidomycosis is a systemic form of mycosis that spreads hematogenously, secondarily to reactivation of lung infection or infection at another site or to new exposure to the causative agent. few cases of bone involvement have been reported in the literature and involvement of the spine is extremely rare. | 2016 | 27355801 |
| chylothorax in paracoccidioidomycosis. | a previously healthy, 52-year-old woman presented with a nine months history of low fever and weight loss (> 30 kg). physical examination disclosed generalized lymphadenopathy, skin lesions, abdominal distension, mild tachypnea and a left breast mass. laboratory tests showed anemia; (prerenal) kidney injury, low serum albumin level; and negative serology for hiv and viral hepatitis. computed tomography (neck/chest/abdomen) showed generalized lymph node enlargement, splenomegaly, pleural effusion ... | 2016 | 27410917 |
| current strategies for diagnosis of paracoccidioidomycosis and prospects of methods based on gold nanoparticles. | paracoccidioidomycosis (pcm) is a human systemic granulomatous mycosis caused by thermodimorphic fungi from paracoccidioides genus. the disease is prevalent in latin america and triggers a serious clinical condition. consequently, rapid diagnosis and treatment are crucial to prevent progression of the disease, which can result in death. currently, there are several established methods for pcm diagnosis. however, many of these tests still present challenges in terms of cost, accessibility and eff ... | 2016 | 27416765 |
| evaluation of the efficacy of antifungal drugs against paracoccidioides brasiliensis and paracoccidioides lutzii in a galleria mellonella model. | paracoccidioides brasiliensis and p. lutzii belong to a group of thermodimorphic fungi and cause paracoccidioidomycosis (pcm), which is a human systemic mycosis endemic in south and central america. patients with this mycosis are commonly treated with amphotericin b (amb) and azoles. the study of fungal virulence and the efficacy and toxicity of antifungal drugs has been successfully performed in a galleria mellonella infection model. in this work, g. mellonella larvae were infected with two par ... | 2016 | 27444116 |
| paracoccin induces m1 polarization of macrophages via interaction with tlr4. | the fungal human pathogen paracoccidioides brasiliensis contains paracoccin (pcn), a multi-domain protein that has lectin and n-acetyl-glucosaminidase activities, which account for its effects on the growth and morphogenesis of the fungus and on the activation of host macrophages through its interaction with tlr n-glycans. with the purpose of detailing the knowledge on the effects of pcn on macrophages, we used recombinant pcn expressed in pichia pastoris (p-rpcn) to stimulate isolated murine pe ... | 2016 | 27458431 |
| extracellular vesicle-associated transitory cell wall components and their impact on the interaction of fungi with host cells. | classic cell wall components of fungi comprise the polysaccharides glucans and chitin, in association with glycoproteins and pigments. during the last decade, however, system biology approaches clearly demonstrated that the composition of fungal cell walls include atypical molecules historically associated with intracellular or membrane locations. elucidation of mechanisms by which many fungal molecules are exported to the extracellular space suggested that these atypical components are transito ... | 2016 | 27458437 |
| infection with paracoccidioides brasiliensis induces b-1 cell migration and activation of regulatory t cells. | paracoccidioidomycosis is a systemic mycosis endemic to latin america. the infection is initiated by inhalation of conidia into the lung and may develop as localized or disseminated disease depending on the depression of cellular immunity. in the present study, we observed that intratracheal infection with paracoccidioides brasiliensis caused the percentage of resident b-1 cells to decrease. using xid mice reconstituted with b-1 cells, we observed that b-1 cells migrate to the spleen and stimula ... | 2016 | 27521490 |
| the cell wall-associated proteins in the dimorphic pathogenic species of paracoccidioides. | paracoccidioides brasiliensis and p. lutzii cause human paracoccidioidomycosis (pcm). they are dimorphic ascomycetes that grow as filaments at mild temperatures up to 28°c and as multibudding pathogenic yeast cells at 37°c. components of the fungal cell wall have an important role in the interaction with the host because they compose the cell outermost layer. the paracoccidioides cell wall is composed mainly of polysaccharides, but it also contains proportionally smaller rates of proteins, lipid ... | 2016 | 27526924 |
| phosphosite-specific regulation of the oxidative-stress response of paracoccidioides brasiliensis: a shotgun phosphoproteomic analysis. | paracoccidioides brasiliensis, a thermally dimorphic fungus, is the causative agent of paracoccidioidomycosis, a systemic mycosis that is widespread in latin america. this fungus is a facultative intracellular pathogen able to survive and replicate inside non-activated macrophages. therefore, the survival of p. brasiliensis inside the host depends on the ability to adapt to oxidative stress induced by immune cells, especially alveolar macrophages. for several years, reactive oxygen species (ros) ... | 2017 | 27590702 |
| real time polymerase chain reaction (rt-pcr): a new patent to diagnostic purposes for paracoccidioidomycosis. | paracoccidioidomycosis (pcm) is a systemic mycosis caused by dimorphic fungi paracoccidioides brasiliensis and p. lutzii. it is prevalent in latin american, mainly in brazil. therefore, pcm has fundamental impact in the brazilian global economy, especially in public health system, since it is affecting economical active population in different country regions. | 2016 | 27593543 |
| detrimental effect of fungal 60-kda heat shock protein on experimental paracoccidioides brasiliensis infection. | the genus paracoccidioides comprises species of dimorphic fungi that cause paracoccidioidomycosis (pcm), a systemic disease prevalent in latin america. here, we investigated whether administration of native 60-kda heat shock protein of p. brasiliensis (npbhsp60) or its recombinant counterpart (rpbhsp60) affected the course of experimental pcm. mice were subcutaneously injected with npbhsp60 or rpbhsp60 emulsified in complete's freund adjuvant (cfa) at three weeks after intravenous injection of p ... | 2016 | 27598463 |
| amphotericin b lipid complex in the treatment of severe paracoccidioidomycosis: a case series. | amphotericin b deoxycholate is the main option for intravenous (i.v.) treatment of severe paracoccidioidomycosis (pcm). this is the first report of amphotericin b lipid complex (ablc) in the treatment of pcm. among 28 patients, cure was achieved in all patients (100%) using ablc. mean and median daily doses of ablc were 3.39 mg/kg/day and 3.35 mg/kg/day, respectively. ablc may be a choice in the treatment of severe forms of pcm or when i.v. options are required. | 2016 | 27612594 |
| identification and characterisation of elongation factor tu, a novel protein involved in paracoccidioides brasiliensis-host interaction. | paracoccidioides spp., which are temperature-dependent dimorphic fungi, are responsible for the most prevalent human systemic mycosis in latin america, the paracoccidioidomycosis. the aim of this study was to characterise the involvement of elongation factor tu (ef-tu) in paracoccidioides brasiliensis-host interaction. adhesive properties were examined using recombinant pbef-tu proteins and the respective polyclonal anti-rpbef-tu antibody. immunogold analysis demonstrated the surface location of ... | 2016 | 27634774 |
| therapeutic effect of monophosphoryl lipid a administration on paracoccidioides brasiliensis-infected mice. | the lack of antifungals with low toxicity and short-term therapy for patients with paracoccidioidomycosis (pcm) led us to evaluate adjuvants in immunotherapeutic intervention. we have previously shown complete freund's adjuvant (cfa) to be therapeutic on experimental pcm. owing to cfa toxicity, here we tested adjuvants approved for clinical use or in preclinical phase in experimental mouse pcm. of all, only monophosporyl lipid a (mpla) demonstrates a beneficial effect, by reducing the fungal bur ... | 2016 | 27639241 |
| depletion of neutrophils exacerbates the early inflammatory immune response in lungs of mice infected with paracoccidioides brasiliensis. | neutrophils predominate during the acute phase of the paracoccidioides brasiliensis infection. herein, we determined the role of the neutrophil during the early stages of experimental pulmonary paracoccidioidomycosis using a monoclonal antibody (mab) specific for neutrophils. male balb/c mice were inoculated intranasally with 1.5 × 10(6) or 2 × 10(6) p. brasiliensis yeast cells. the mab was administered 24 h before infection, followed by doses every 48 h until mice were sacrificed. survival time ... | 2016 | 27642235 |
| depletion of neutrophils promotes the resolution of pulmonary inflammation and fibrosis in mice infected with paracoccidioides brasiliensis. | chronic stages of paracoccidioidomycosis (pcm) are characterized by granulomatous lesions which promote the development of pulmonary fibrosis leading to the loss of respiratory function in 50% of patients; in addition, it has been observed that neutrophils predominate during these chronic stages of p. brasiliensis infection. the goal of this study was to evaluate the role of the neutrophil during the chronic stages of experimental pulmonary pcm and during the fibrosis development and tissue repa ... | 2017 | 27690127 |
| hiv immune recovery inflammatory syndrome and central nervous system paracoccidioidomycosis. | the immune reconstitution inflammatory syndrome (iris) is a deregulated inflammatory response to invading microorganisms. it is manifested when there is an abrupt change in host immunity from an anti-inflammatory and immunosuppressive state to a pro-inflammatory state as a result of rapid depletion or removal of factors that promote immune suppression or inhibition of inflammation. the aim of this paper is to discuss and re-interpret the possibility of association of paracoccidioidomycosis (pcm) ... | 2017 | 27718161 |
| severe changes in thymic microenvironment in a chronic experimental model of paracoccidioidomycosis. | t cell maturation takes place within the thymus, a primary lymphoid organ that is commonly targeted during infections. previous studies showed that acute infection with paracoccidioides brasiliensis (pb), the causative agent of paracoccidioidomycosis (pcm), promotes thymic atrophy that is associated with the presence of yeast cells in the organ. however, as human pcm is a chronic infection, it is imperative to investigate the consequences of pb infection over the thymic structure and function in ... | 2016 | 27736987 |
| paracoccidioides brasiliensis-associated dermatitis and lymphadenitis in a dog. | paracoccidioidomycosis (pcm) is an endemic disease of humans from latin america that is caused by paracoccidioides brasiliensis and p. lutzii, with most cases of pcm in domestic animals being associated with p. brasiliensis. this study presents the clinical, cytological, mycological, serological, and molecular findings associated with p. brasiliensis in a dog from southern brazil. fine needle biopsies were collected from the skin and several lymph nodes of a 5-year-old female labrador dog that h ... | 2017 | 27757778 |
| extracellular vesicles from paracoccidioides brasiliensis induced m1 polarization in vitro. | extracellular vesicles (evs) released by eukaryotes, archaea, and bacteria contain proteins, lipids, polysaccharides, and other molecules. the cargo analysis of evs shows that they contain virulence factors suggesting a role in the pathogenesis of infection. the proteome, lipidome, rna content, and carbohydrate composition of evs from paracoccidioides brasiliensis and paracoccidioides lutzii were characterized. however, the effects of p. brasiliensis evs on the host immune system have not yet be ... | 2016 | 27775058 |
| cutaneous granulomas in dolphins caused by novel uncultivated paracoccidioides brasiliensis. | cutaneous granulomas in dolphins were believed to be caused by lacazia loboi, which also causes a similar disease in humans. this hypothesis was recently challenged by reports that fungal dna sequences from dolphins grouped this pathogen with paracoccidioides brasiliensis. we conducted phylogenetic analysis of fungi from 6 bottlenose dolphins (tursiops truncatus) with cutaneous granulomas and chains of yeast cells in infected tissues. kex gene sequences of p. brasiliensis from dolphins showed 10 ... | 2016 | 27869614 |
| paracoccidioidomycosis in brazilian patients with and without human immunodeficiency virus infection. | paracoccidioidomycosis (pcm) is endemic to latin america, where 10 million people may be infected with paracoccidioides brasiliensis/paracoccidioides lutzii and 1,600,000 individuals live with human immunodeficiency virus (hiv) infection. an epidemiological overlapping of these infections occurred early in acquired immunodeficiency syndrome era with nearly 180 published cases. this study presents epidemiological, clinical, and outcome profiles for 31 pcm patients with hiv infection diagnosed in ... | 2017 | 27895278 |
| tolerogenic plasmacytoid dendritic cells control paracoccidioides brasiliensis infection by inducting regulatory t cells in an ido-dependent manner. | plasmacytoid dendritic cells (pdcs), considered critical for immunity against viruses, were recently associated with defense mechanisms against fungal infections. however, the immunomodulatory function of pdcs in pulmonary paracoccidiodomycosis (pcm), an endemic fungal infection of latin america, has been poorly defined. here, we investigated the role of pdcs in the pathogenesis of pcm caused by the infection of 129sv mice with 1 x 106 p. brasiliensis-yeasts. in vitro experiments showed that p. ... | 2016 | 27992577 |
| chronic paracoccidioidomycosis with adrenal involvement mimicking tuberculosis - a case report from austria. | paracoccidioidomycosis is a systemic fungal infection caused by paracoccidioides brasiliensis and endemic in certain areas of central and south america. we report a case of a 62-year-old-man with a complex history of tuberculosis and imaging findings of a cerebral lesion and bilateral adrenal enlargement. biopsy of adrenal gland revealed paracoccidioides brasiliensis. this case highlights the importance of travel history for diagnosis of paracoccidioidomycosis in non-endemic areas and emphasizes ... | 2016 | 27995053 |
| paracoccidioides brasiliensis induces cytokine secretion in epithelial cells in a protease-activated receptor-dependent (par) manner. | paracoccidioides brasiliensis is one of the etiological agents of the human systemic mycosis paracoccidioidomycosis. protease-activated receptors (pars) are expressed in many cell types and comprise a family of g protein-coupled receptors (par-1, par-2, and par-4), which may be activated by proteases secreted by several pathogens. in the present study, we showed that the pathogenic fungus p. brasiliensis secretes components that promote interleukin (il)-6 and il-8 secretion by the lung epithelia ... | 2017 | 27995367 |
| photodynamic inactivation of oral paracoccidioidomycosis affecting woman with systemic lupus erythematosus: an unusual case report. | paracoccidioidomycosis (pcm) is a systemic disease caused by the dimorphic fungus paracocdioides brasiliensis. the infection primarily reaches the lungs by the inhalation of fungi spores and later can disseminate to other organs causing secondary oral lesions. systemic lupus erythematosus (sle), on the other hand, is an autoimmune chronic inflammatory disease that affects various organ systems, including skin and oral cavity. here we report a 39-year-old female patient bearing sle and presenting ... | 2017 | 28012807 |
| acute pulmonary involvement by paracoccidiodomycosis disease immediately after kidney transplantation: case report and literature review. | paracoccidioides brasiliensis is the cause of paracoccidioidomycosis, one of the most important systemic mycoses in latin america. human disease has been observed in a limited geographic and ecological niche, and it is attributed to exposure to the fungus in soil. most primary infections are subclinical, as the infection is contained by the host mainly through cell-mediated immune response. however, as the fungus has the ability to survive in a dormant state for long periods, an impairment of th ... | 2017 | 28039947 |
| study of differential expression of mirnas in lung tissue of mice submitted to experimental infection by paracoccidioides brasiliensis. | micrornas (mirnas) are small single stranded rna sequences involved in post-transcriptional regulation of different biological and physiological processes. paracoccidioidomycosis (pcm) is an infection caused by paracoccidioides brasiliensis, and it is a major cause of mortality due to systemic mycoses in brazil. to date, there have been few reports on the role of mirnas in the immune response against fungi, especially pcm. the objective of this study was to evaluate the differential expression o ... | 2017 | 28053145 |
| peptides derived from a phage display library inhibit adhesion and protect the host against infection by paracoccidioides brasiliensis and paracoccidioides lutzii. | paracoccidioides brasiliensis and paracoccidioides lutzii are dimorphic fungi and are the etiological agents of paracoccidioidomycosis (pcm). adhesion is one of the most important steps in infections with paracoccidioides and is responsible for the differences in the virulence of isolates of these fungi. because of the importance of adhesion to the establishment of an infection, this study focused on the preliminary development of a new therapeutic strategy to inhibit adhesion by paracoccidioide ... | 2016 | 28066254 |
| effects of two 6-quinolinyl chalcones on the integrity of plasma membrane of paracoccidioides brasiliensis. | paracoccidioidomycosis is the most prevalent systemic mycosis in latin america, yet few therapeutic options exist. our aim was to search for new compounds with high efficacy, low toxicity, shorter treatment time and affordable cost. we studied two synthetic 6-quinolinyl chalcones, 3b and 3e, to determine their effects on vero cells, antifungal activity, survival curve, interaction with other drugs and phenotypic effects against several isolates of paracoccidioides spp. in this study, we verified ... | 2017 | 28074055 |
| use of fluorescent oligonucleotide probes for differentiation between paracoccidioides brasiliensis and paracoccidioides lutzii in yeast and mycelial phase. | fluorescence in situ hybridisation (fish) associated with tyramide signal amplification (tsa) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible to culture. | 2017 | 28177048 |
| standardization and validation of dot-elisa assay for paracoccidioides brasiliensis antibody detection. | paracoccidioidomycosis (pcm) is a neglected systemic mycosis caused by a dimorphic fungus of the paracoccidioides genus. the standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. however, in some situations, access to the site of injury prevents the collection of biological material. a variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a ... | 2017 | 28239394 |
| miltefosine is fungicidal to paracoccidioides spp. yeast cells but subinhibitory concentrations induce melanisation. | paracoccidioidomycosis (pcm) is a systemic mycosis caused by the dimorphic fungi paracoccidioides spp. the duration of antifungal treatment ranges from months to years and relapses may nevertheless occur despite protracted therapy. thus, there remains an urgent need for new therapeutic options. miltefosine (mlt), an analogue of alkylphospholipids, has antifungal activity against species of yeast and filamentous fungi. the aim of this study was to evaluate the antifungal effects of mlt on the yea ... | 2017 | 28279786 |
| animal models and antifungal agents in paracoccidioidomycosis: an overview. | paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis, the most prevalent systemic mycosis in latin america. the morbidity and mortality associated with paracoccidioidomycosis necessitate our understanding of fungal pathogenesis and discovering of new agents to treat this infection. animal models have contributed much to the knowledge of fungal infections and their corresponding therapeutic treatments. this is true for animal models of the primary fungal pathogens such a ... | 2017 | 28324244 |
| anti-apoptotic effects of decyl gallate on the induction of apoptosis in a549 pneumocytes by paracoccidioides brasiliensis gp43. | apoptosis is considered an escape mechanism from the host immune system for the fungus paracoccidioides spp, and it serves as a vehicle for entry into macrophages without stimulating microbicidal activities. recently, gp43 of p. brasiliensis was demonstrated to be involved in this process. therefore, as a new therapeutic alternative, it is very important to study compounds that could reduce the modulation of the induction of apoptosis caused by this fungus. decyl gallate (g14) is a known antifun ... | 2017 | 28339963 |
| linear epitopes of paracoccidioides brasiliensis and other fungal agents of human systemic mycoses as vaccine candidates. | dimorphic fungi are agents of systemic mycoses associated with significant morbidity and frequent lethality in the americas. among the pathogenic species are paracoccidioides brasiliensis and paracoccidioides lutzii, which predominate in south america; histoplasma capsulatum, coccidioides posadasii, and coccidioides immitis, and the sporothrix spp. complex are other important pathogens. associated with dimorphic fungi other important infections are caused by yeast such as candida spp. and crypto ... | 2017 | 28344577 |
| photodynamic inactivation of paracoccidioides brasiliensis helps the outcome of oral paracoccidiodomycosis. | the antifungal drug therapy often employed to treat paracoccidiodomycosis (pcm), an important neglected fungal systemic infection, leads to offensive adverse effects, besides being very long-lasting. in addition, pcm compromises the oral health of patients by leading to oral lesions that are very painful and disabling. in that way, photodynamic therapy (pdt) arises as a new promising adjuvant treatment for inactivating paracoccidioides brasiliensis (pb), the responsible fungus for pcm, and also ... | 2017 | 28349345 |
| acute juvenile paracoccidioidomycosis: a 9-year cohort study in the endemic area of rio de janeiro, brazil. | paracoccidioidomycosis (pcm) is a systemic mycosis caused by pathogenic dimorphic fungi of the genus paracoccidioides. it is the most important systemic mycosis in latin america and the leading cause of hospitalizations and death among them in brazil. acute pcm is less frequent but relevant because vulnerable young patients are affected and the severity is usually higher than that of the chronic type. | 2017 | 28355221 |
| recombinant 60-kda heat shock protein from paracoccidioides brasiliensis: is it a good antigen for serological diagnosis of paracoccidioidomycosis? | paracoccidioides brasiliensis and p. lutzii are fungi that cause paracoccidioidomycosis (pcm), the most prevalent systemic mycosis in south america. for serological diagnosis, although 43-kda glycoprotein (gp43) is regarded as highly specific for pcm, the occurrence of false negative reactions in sera from patients infected with p. lutzii suggests that preparation with only one antigen is not recommended. heat shock proteins are feasible alternatives as a second antigen because they are often hi ... | 2017 | 28380215 |
| unusual otolaryngologic manifestations of paracoccidioidomycosis: a case report and review of literature. | abstractparacoccidioidomycosis is a systemic mycosis caused by paracoccidioides brasiliensis. it occurs more frequently in its chronic form, which particularly affects male adults from rural areas. these patients present with pulmonary involvement and systemic symptoms. skin and mucosal lesions are rather typical and might suggest the diagnosis. the involvement of the upper airway mucosa is common and the patients usually complain of dysphagia and dysphonia. nonetheless, in endemic areas, physic ... | 2017 | 28500805 |
| serological evidence of infection by paracoccidioides brasiliensis in dogs with leishmaniasis. | paracoccidioidomycosis is a systemic mycosis prevalent in latin american countries, caused by the dimorphic fungi paracoccidioides brasiliensis and p. lutzii. the habitat of these fungi in nature remains undefined, although it is believed that infection occurs by inhalation of infective propagules present in soil. sentinel animals, such as dogs, can be valuable epidemiological markers of paracoccidioidomycosis. taking into account that paracoccidioidomycosis and visceral leishmaniasis may occur ... | 2017 | 28523421 |
| chronic paracoccidioidmycosis in a woman with crohn disease. | we report a rare case of chronic paracoccidioidomycosis(pcm) in a woman with crohn disease in the setting of treatment with azathioprine and mesalazine. serum tests for antigens to paracoccidioides brasiliensis, histoplasma capsulatum, and aspergillus fumigatus were negative. an incisional biopsy of an oral lesion with periodic acid-schiff and grocott-methenamine silver stains revealed chronic granulomatous inflammation with multinucleated giant cells with paracoccidioides brasiliensis within th ... | 2017 | 28541872 |
| hepatic disease with portal hypertension and acute juvenile paracoccidioidomycosis: a report of two cases and literature review. | paracoccidioidomycosis (pcm) is a neglected systemic mycosis endemic to latin america caused by dimorphic fungi of the genus paracoccidioides. the acute juvenile pcm is a severe type of presentation that usually affects young vulnerable patients and rarely progresses to portal hypertension. here, two cases of liver disease and portal hypertension as complications of acute juvenile pcm are reported. diagnosis of pcm was performed by isolation of the fungus and molecular identification of the stra ... | 2017 | 28577123 |
| mouse immunization with radioattenuated yeast cells of paracoccidioides brasiliensis. | paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent mycosis in latin america, and currently there is no effective vaccine. the present chapter describes the methodology to obtain radioattenuated yeast cells of p. brasiliensis and a protocol to evaluate protective response elicited by this immunogen in experimental paracoccidioidomycosis. the radioattenuated yeast provides a valuable tool for immunological studies in experimental paracoccidioidomycosis and vac ... | 2017 | 28584979 |
| dnahsp65 vaccine as therapy against paracoccidioidomycosis. | the conventional treatment for fungal diseases usually shows long periods of therapy and the high frequency of relapses and sequels. new strategies of the treatment are necessary. we have shown that the mycobacterium leprae hsp65 gene can be successfully used as therapy against murine paracoccidioidomycosis (pcm). here, we described the methodology of dnahsp65 immunotherapy in mice infected with the dimorphic fungus paracoccidioides brasiliensis, one of pcm agent, evaluating cytokines levels, fu ... | 2017 | 28584985 |
| peptide vaccine against paracoccidioidomycosis. | the chapter reviews methods utilized for the isolation and characterization of a promising immunogen candidate, aiming at a human vaccine against paracoccidioidomycosis. peptide p10 carries a t-cd4+ epitope and was identified as an internal sequence of the major diagnostic antigen known as gp43 glycoprotein. it successfully treated massive intratracheal infections by virulent paracoccidioides brasiliensis in combination with chemotherapy.an introduction about the systemic mycosis was found essen ... | 2017 | 28584987 |
| yeast expressing gp43 protein as a vaccine against paracoccidioides brasiliensis infection. | paracoccidioidomycosis (pcm) represents the most frequent systemic mycosis in latin american. the disease is caused by the pathogenic thermally dimorphic fungus paracoccidioides brasiliensis, and is initially characterized by pulmonary lesions, which can subsequently disseminate to other organs, resulting in secondary injuries. although its high incidence, there is no commercially available vaccine against fungal diseases. a novel strategy, using saccharomyces cerevisiae yeast as a vehicle for i ... | 2017 | 28584992 |
| the critical role of notch1-tlr 4 signaling in the inflammatory and fungicidal activity of macrophages against paracoccidioides brasiliensis strain pb18. | paracoccidioidomycosis is a systemic mycosis of deep nature that primarily affects the lung and can spread via lymphatic and hematogenous to other organs and tissues. it is mainly caused by paracoccidioides brasiliensis fungus which exhibits thermal dimorphism. the innate immune system mediated by macrophages is extremely important for the control of infection and is involved in the induction and regulation of immune/inflammatory response. these cells are able to recognize pathogens through patt ... | 2017 | 28600728 |
| imaging spectrum of invasive fungal and fungal-like infections. | invasive fungal and fungal-like infections contribute to substantial morbidity and mortality in immunocompromised individuals. the incidence of these infections is increasing-largely because of rising numbers of immunocompromised patients, including those with neutropenia, human immunodeficiency virus, chronic immunosuppression, indwelling prostheses, burns, and diabetes mellitus, and those taking broad-spectrum antibiotics. invasive fungal pathogens include primary mycotic organisms such as his ... | 2017 | 28622118 |
| can passage in galleria mellonella activate virulence factors of paracoccidioides brasiliensis as in the murine model? | paracoccidioidomycosis (pcm) is a fungal disease restricted to latin countries, and its etiologic agents derive from the paracoccidioides genus. attenuation or loss of virulence in paracoccidioides spp. following successive subculturing has been described. however, virulence can be recovered by passage in mammalian host. in this study, the recovery of adhesion of p. brasiliensis through passage in mice was compared to that in the insect galleria mellonella. analysis of in vitro fungal-host cell ... | 2017 | 28637229 |
| standardization and prevalence of the booster phenomenon: evaluation using a two-step skin test with 43 kda glycoprotein in individuals from an endemic region of paracoccidioidomycosis. | we estimated the occurrence rate of the booster phenomenon by using an intradermal test with 43 kda glycoprotein in an endemic area of paracoccidioidomycosis in the central-west region of brazil. | 2017 | 28646277 |
| targeting the homoserine dehydrogenase of paracoccidioides species for treatment of systemic fungal infections. | this work evaluated new potential inhibitors of the enzyme homoserine dehydrogenase (hsd) of paracoccidioides brasiliensis, one of the etiological agents of paracoccidioidomycosis. the tertiary structure of the protein bonded to the analogue nad, and l-homoserine was modeled by homology. the model with the best output was subjected to gradient minimization, redocking, and molecular dynamics simulation. virtual screening simulations with 187,841 molecules purchasable from the zinc database were p ... | 2017 | 28652239 |
| alkyl protocatechuate-loaded nanostructured lipid systems as a treatment strategy for paracoccidioides brasiliensis and paracoccidioides lutzii in vitro. | dodecyl protocatechuate (dodecyl) is a derivative of protocatechuic acid (3,4-dihydroxybenzoic acid) that possesses anti-oxidant and antifungal properties. nanostructured lipid systems (nls) can potentiate the action of many antifungal agents, reducing the required dose and side effects by improving their activity. this work aimed to evaluate dodecyl protocatechuate loaded into a nls (nls+dodecyl) as a strategy for the treatment of paracoccidioides brasiliensis and p. lutzii in vitro. antifungal ... | 2017 | 28659880 |
| dendritic cells primed with paracoccidioides brasiliensis peptide p10 are therapeutic in immunosuppressed mice with paracoccidioidomycosis. | paracoccidioidomycosis (pcm) is an endemic systemic mycosis in latin america, with the highest prevalence in brazil, colombia, and venezuela. fungi of the paracoccidioides genus are etiologic agents of the disease. the 15 amino acid peptide p10 is derived from gp43, the main diagnostic antigen of paracoccidioides brasiliensis. we previously reported that p10-pulsed dendritic cells (dcs) induce a protective response against p. brasiliensis. presently, dexamethasone-treated balb/c mice were intrat ... | 2017 | 28659882 |
| depicting the metabolism of paracoccidioides brasiliensis during infection by transcriptional and proteomic approaches. | 2017 | 28679067 | |
| paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection. | paracoccidoides brasiliensis and paracoccidioides lutzii, the etiologic agents of paracoccidioidomycosis, cause disease in healthy and immunocompromised persons in latin america. we developed a method for harvesting p. brasiliensis yeast cells from infected murine lung to facilitate in vivo transcriptional and proteomic profiling. p. brasiliensis harvested at 6 h post-infection were analyzed using rnaseq and lc-ms(e). in vivo yeast cells had 594 differentially expressed transcripts and 350 diffe ... | 2017 | 28704618 |
| impact of paracoccin gene silencing on paracoccidioides brasiliensis virulence. | among the endemic deep mycoses in latin america, paracoccidioidomycosis (pcm), caused by thermodimorphic fungi of the paracoccidioides genus, is a major cause of morbidity. disease development and its manifestations are associated with both host and fungal factors. concerning the latter, several recent studies have employed the methodology of gene modulation in p. brasiliensis using antisense rna (asrna) and agrobacterium tumefaciens-mediated transformation (atmt) to identify proteins that influ ... | 2017 | 28720727 |
| health and environmental risk assessment project for bottlenose dolphins tursiops truncatus from the southeastern usa. i. infectious diseases. | from 2003 to 2015, 360 free-ranging atlantic bottlenose dolphins tursiops truncatus inhabiting the indian river lagoon (irl, n = 246), florida, and coastal waters of charleston (chs, n = 114), south carolina, usa, were captured, given comprehensive health examinations, and released as part of a multidisciplinary and multi-institutional study of individual and population health. the aim of this review is to summarize the substantial health data generated by this study and to examine morbidity bet ... | 2017 | 28737159 |
| nod-like receptor p3 inflammasome controls protective th1/th17 immunity against pulmonary paracoccidioidomycosis. | the nod-like receptor p3 (nlrp3) inflammasome is an intracellular multimeric complex that triggers the activation of inflammatory caspases and the maturation of il-1β and il-18, important cytokines for the innate immune response against pathogens. the functional nlrp3 inflammasome complex consists of nlrp3, the adaptor protein apoptosis-associated speck-like protein, and caspase-1. various molecular mechanisms were associated with nlrp3 activation including the presence of extracellular atp, rec ... | 2017 | 28740491 |