Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| growth and development of water buffalo and friesian crossbred cattle, with special reference to the 'entire' and 'boneless' cuts. | twelve buffaloes, nine friesian × baladi and nine friesian × (friesian × baladi) bulls were slaughtered over the live weight ranges 161-560 kg for buffaloes and 176-448 kg for cattle. right sides of all carcasses were jointed and dissected and the increase in the weight of 'entire' and 'boneless' cuts and cut groups (i.e. pistol; blrc) relative to the 'entire' and 'boneless' side weights, respectively, were examined using covariance analyses. increasing distoproximal and dorsoventral growth grad ... | 1982 | 22054989 |
| tissue growth patterns in the carcasses of water buffalo and friesian crossbred cattle-part 1: individual muscles and anatomical muscle groups. | the left sides of the carcasses of twelve egyptian buffalo, nine half friesian (? friesian x ? egyptian baladi) and nine three-quarter friesian (? x ? half-friesian) bulls, serially slaughtered between 161 and 560 kg for buffaloes and between 176 and 448 kg for cattle, were used to describe the growth and distribution of individual muscles and muscle groups. genotype-group differences in the relative rate of growth were not significant in 85 of 89 muscles and in 8 of 9 muscle groups. the pooled ... | 1983 | 22055628 |
| fiber types in the longissimus muscle from water buffalo and selected domestic beef breeds. | the distribution and area of longissimus muscle fiber types from two intact male water buffaloes, one angus bull and one charolais bull were evaluated. only fibers of aerobic-oxidative capacity were present in the muscles of the water buffalo examined. the absence of aw fibers may imply some significant and distinct differences in muscle metabolic and functional characteristics. all three fibers types were present in the muscles from the angus and charolais bulls. these findings may implicate di ... | 1985 | 22055630 |
| tissue growth patterns in the carcasses of water buffalo and friesian crossbred cattle: part 2-individual bones and anatomical bone groups. | twelve egyptian buffalo, nine ? friesian × ? egyptian native baladi (half friesian) and nine ? friesian × ? half friesian (three-quarter friesian) bulls were serially slaughtered between 161 and 560 kg for buffaloes and between 176 and 448 kg for cattle. anatomical dissection data from the left sides of the carcasses were used to examine the growth of individual bones and bone groups. no significant genetic differences with respect to the growth patterns of bones relative to total side bone were ... | 1983 | 22055706 |
| polymerase chain reaction assay for detection of sheep and goat meats. | polymerase chain reaction (pcr) was applied to a qualitative differentiation between sheep, goat and bovine meats. oligonucleotide primers were designed for the amplification of sheep satellite i dna sequence. the pcr amplified 374 bp fragments from sheep and goat dna, but no fragment from bovine, water buffalo, sika deer, pig, horse, rabbit and chicken dna. sheep dna (10 pg) was detected by 4% agarose gel electrophoresis following pcr amplification. althoug cooking of the sample meats reduced t ... | 1994 | 22059540 |
| effect of electrical stimulation on post mortem biochemical characteristics and quality of longissimus dorsi thoracis muscle from buffalo (bubalus bubalis). | high voltage electrical stimulation (700 v, 1400 v peak, pulses 1 s on/1 s off, 60 hz, 2 a) on buffalo carcasses resulted in a significantly more rapid ph fall in longissimus dorsi thoracis muscle when compared to non stimulated controls (p < 0.01), during the first 24 h after slaughter. the imp atp ratio on the same period showed a much more rapid increase for the stimulated muscles (1.07 and 1.16 at times 1 and 2.5 hpost mortem vs control values of 0.77 and 0.83, respectively). sensory and in ... | 1995 | 22060207 |
| effect of cooking and storage on lipid oxidation and development of cholesterol oxidation products in water buffalo meat. | buffalo meat was subjected to two cooking methods viz. broiling and pressure cooking and two storage procedures viz. refrigerated (4 °c) storage for six days and frozen (-10 °c) storage for 90 days. changes in lipid oxidation and development of cholesterol oxidation products were studied in raw as well as cooked meat samples. total lipid, phospholipid, cholesterol, free fatty acid, glycolipid and glyceride contents increased significantly on cooking of meat but did not show any significant chang ... | 1996 | 22060572 |
| sequence analysis of mitochondrial 12s rrna gene can identify meat species. | in this study, sequence analysis of mitochondrial 12s rrna has been applied for meat species identification. the procedure involves polymerase chain reaction (pcr) amplification of a fragment of mitochondrial (mt) 12s rrna gene and sequencing of amplicons. amplified product of mt 12s rrna gene was 456 bp in size. species sequenced include cattle (bos indicus), buffalo (bubalus bubalis), sheep (ovis aries), goat (capra hircus) and mithun (bos frontalis). sequences were compared with the reported ... | 2004 | 22060864 |
| protease activity higher in postmortem water buffalo meat than brahman beef. | we previously demonstrated that postmortem water buffalo meat had higher tenderness than brahman beef. in order to explain this difference in tenderness, the objective of the current study was to investigate the protease activity in these two meats. five female crossbred water buffalo (philippine carabao×bulgarian murrah) and five female crossbred cattle (brahman×philippine native) were slaughtered at 30months of age, followed by immediate sampling of longissimus thoracis muscle for measurement ... | 2007 | 22061792 |
| occurrence of conjugated linoleic acid in longissimus dorsi muscle of water buffalo (bubalus bubalis) and zebu-type cattle raised under savannah conditions. | lipid extracts from longissimus dorsi muscles of 64 water buffaloes and 68 zebu-type cattle were used to quantify the amount (mg/g of lipids) of total conjugated linoleic acid (cla), cla isomers c9, t11 and t10, c12 and linoleic acid (la), according to species (buffaloes and cattle), age (slaughter groups at 7, 17, 19 or 24 months of age) and gender (bulls and steers). the effects of gender and age were significant (p<0.05) but marginal. comparisons of lipid extracts from buffaloes vs. cattle sh ... | 2005 | 22062643 |
| false aneurysms in carotid arteries of cattle and water buffalo during shechita and halal slaughter. | it has previously been shown that the cattle brain is supplied with blood via a basi-occipital plexus, in addition to branches from the carotid and basilar arteries. in addition it has been shown during conventional stunning and slaughter that the carotid arteries in cattle can develop false aneurysms at their severed ends and this can curtail exsanguination. this investigation examined whether false aneurysms can occur during religious slaughter, and during bleeding following electrical stunnin ... | 2008 | 22062756 |
| effect of aging on the characteristics of meat from water buffalo grown in the delta del paraná region of argentina. | fifteen crossbreed water buffalos were selected from a farm in delta del paraná, entre ríos province, argentina. animals were castrated males 20-24 months old reaching final live weights of 400-420kg. they were predominantly of mediterranean and murrah breeds and were feed in naturally grown pastures. tenderness and chewiness increased with postmortem aging (p<0.05). aging did not affect flavour and odour scores, even though certain off-flavours and off-odours were reported. changes in colour wi ... | 2008 | 22062913 |
| animal welfare at markets and during transport and slaughter. | this review highlights some recent developments in our understanding of stress and physical injuries that occur before and during transport to slaughter, during handling at livestock markets, and at the time animals are put-up for slaughter within abattoirs. stress in pigs during transfer to the stunning point within the abattoir has important effects on meat quality, and there is growing evidence that strenuous exercise or co(2) stunning can contribute to oxidative rancidity in pigs, poultry an ... | 2008 | 22063164 |
| aluminium contents in baked meats wrapped in aluminium foil. | in this investigation, the effect of cooking treatments (60min at 150°c, 40min at 200°c, and 20min at 250°c) on aluminium contents of meats (beef, water buffalo, mutton, chicken and turkey) baked in aluminium foil were evaluated. cooking increased the aluminium concentration of both the white and red meats. the increase was 89-378% in red meats and 76-215% in poultry. the least increase (76-115%) was observed in the samples baked for 60min at 150°c, while the highest increase (153-378%) was in s ... | 2006 | 22063217 |
| authentication of meat from game and domestic species by snapshot minisequencing analysis. | the aim of the present study is to develop an assay for the specific identification of meat from capreolus capreolus, cervus elaphus, capra ibex, rupicapra rupicapra, targeting sequences of the cytochrome b (cyt b) gene of mitochondrial dna. the assay is also intended to enable differentiation between meat from these wild species as well as ovis aries, capra hircus, bubalus bubalis, bos taurus and sus scrofa domestic species. the primers used in the preliminary pcr were designed in well conserve ... | 2008 | 22063325 |
| differentiation of cattle species in beef by pcr-rflp of mitochondrial and satellite dna. | methods currently used for the identification of the species origin of meat or tissue samples have not been validated for other bovine species than taurine cattle or water buffalo. these methods also do not discriminate between the different bovine species that are used as source of beef. here, we describe two complementary methods for detection and differentiation of bovine species, which are based on mutations in mitochondrial dna and centromeric satellite dna, respectively. the analysis of sa ... | 2002 | 22063639 |
| effect of dietary energy content on the intramuscular fat depots and triglyceride composition of river buffalo meat. | the production of river buffalo meat in italy has long been under discussion due to poor acceptance by consumers. in order to understand whether dietary energy content may affect the organoleptic characteristics of buffalo meat, two groups of river buffalo calves were fed on two diets, with high (h) and low (l) energy contents. the animals were slaughtered at 4-monthly intervals starting from 6 months old (10, 14 and 18 months) and five muscles were dissected on the half-carcass: caput longum tr ... | 2003 | 22063782 |
| mineral content of longissimus dorsi thoracis from water buffalo and zebu-influenced cattle at four comparative ages. | the longissimus dorsi thoracis (ldt) of 64 water buffalo and 68 zebu-influenced cattle was used to determine the mineral content variation (mg/100g of fresh tissue) according to species, age (7, 17, 19 and 24 months-of-age, moa) and gender (steers and bulls). buffalo ldt had higher concentrations of k, mn, fe, zn and cu at weaning (p<0.05). phosphorous content was higher (p<0.05) at post-weaning ages. mineral profile was affected by age without clear trends; the na and k contents increased at 24 ... | 2007 | 22063805 |
| difference in tenderness and ph decline between water buffalo meat and beef during postmortem aging. | the objective of this research was to determine the difference in tenderness and some characteristics of water buffalo meat and beef during postmortem aging. five female crossbred water-buffalo (philippine carabao×bulgarian murrah) and five female crossbred cattle (brahman×philippine native), were finished on the same diet for 6 months and slaughtered at 30 months of age. the muscle ph was measured at 40min, 3h, 7h, 24h, and 48h postmortem. longissimus thoracis (lt) and semimembranosus (sm) musc ... | 2007 | 22063807 |
| effectiveness of poll stunning water buffalo with captive bolt guns. | practical experience has indicated that shooting water buffalo with a captive bolt gun in the front of the head does not always produce an effective stun. slaughtermen have been claiming that the poll position is more reliable, but under present eu regulations this shooting position is not allowed for domesticated bovines. this study examined the effectiveness of shooting water buffalo with captive bolt guns in the poll region. the depth of concussion was assessed in 30 water buffalo from physic ... | 2009 | 22063979 |
| some qualitative and chromatic aspects of thawed buffalo (bubalus bubalis) meat. | after thawing, the meat of beef calves (italian frisian breed) and buffalo calves (mediterranean breed) slaughtered at 4, 8 and 12 months of age was examined. both the ph and the thawing loss confirmed that the meat of buffalo calves is more suitable for preservation by freezing. with increased age and time of exposure to air the lightness of the non-renewed surface was reduced. the lightness of the fresh cut surface remained stable in the various thawing phases though it was less in the older a ... | 2007 | 22064306 |
| Buffalo (Bubalus bubalis) Epiphyseal Proteins Counteract Arsenic-Induced Oxidative Stress in Brain, Heart, and Liver of Female Rats. | Arsenic (As) toxicity through induction of oxidative stress is a well-known mechanism of organ toxicity. To address this problem, buffalo epiphyseal proteins (BEP, at 100 µg/kg BW, i.p. for 28 days) were administered intraperitoneally to female Wistar rats exposed to As (100 ppm sodium arsenite via drinking water for 28 days). Arsenic exposure resulted in marked elevation in lipid peroxidation in brain, cardiac, and hepatic tissues, whereas significant (p?<?0.05) adverse change in catalase, supe ... | 2011 | 22095291 |
| derivation, characterization and differentiation of buffalo (bubalus bubalis) amniotic fluid derived stem cells. | amniotic fluid cells (afcs) are obtained from amnion for pre-natal analysis and can be cultured in vitro. heterogeneous amniotic fluid (af) contains various cell types, and it is believed that some of these cells possess the stem cell properties. the aim of this study was to characterize these cells by phenotypical and genotypical means in buffalo. the differentiation potential of amniotic fluid stem (afs) cells was carried out by converting these cells into neurons. the afcs were cultured witho ... | 2011 | 22107067 |
| evaluation of fasting metabolism of growing water buffalo (bubalus, bubalis). | the objectives of the present study were to evaluate fasting metabolism (fm) of water buffalo (bubalus, bubalis) at three stages of growth (12, 18 and 24 months) in guangxi, china. five female water buffalo were used for each age group and their live weight was on average 254, 326 and 338 kg, respectively. all animals were of average body condition, healthy and de-wormed before start of the study. prior to a 6-day fasting period, buffalo were offered a mixed diet of forage and concentrates (70% ... | 2011 | 22111628 |
| molecular cloning and characterization of th1 and th2 cytokines of african buffalo (syncerus caffer). | the african buffalo (syncerus caffer) has been implicated as the reservoir of several bovine infectious agents. however, there is insufficient information on the protective immune responses in the african buffalo, particularly in infected animals. in this study, we analysed th1 cytokines il-2 and ifn-γ, and th2 cytokines il-4 and il-10. the cloned cdna of il-2, il-4, il-10 and ifn-γ contained an open reading frame of 468, 501, 408 and 540 nucleotides, encoding polypeptides of 155, 166, 135 and 1 ... | 2011 | 22117600 |
| comparative proteomics to evaluate multi drug resistance in escherichia coli. | drug resistance in food-borne bacterial pathogens is an almost inevitable consequence of the use of antimicrobial drugs, used either therapeutically or to avoid infections in food-producing animals. in the past decades, the spread and inappropriate use of antibiotics have caused a considerable increase of antibiotics to which bacteria have developed resistance and, moreover, bacteria are becoming resistant to more than one antibiotic simultaneously. understanding mechanisms at the molecular leve ... | 2011 | 22120138 |
| a possible case of caprine-associated malignant catarrhal fever in a domestic water buffalo (bubalus bubalis) in switzerland. | abstract: | 2011 | 22132808 |
| evaluation of indirect tasp enzyme-linked immunosorbent assay for diagnosis of tropical theileriosis in cattle (bos indicus) and water buffaloes (bubalus bubalis) in egypt. | the aim of the present study was to evaluate the validity of theileria annulata surface protein (tasp)-elisa, in comparison with traditional microscopic test, for the diagnosis of t. annulata infection among egyptian baladi cattle (bos taurus) and water buffaloes (bubalus bubalis). molecular confirmation of infection using t. annulata merozoite surface (tams-1) target amplification by pcr was used as a gold standard. a total of 76 clinically suspected animals including 64 baladi cattle and 12 wa ... | 2011 | 22136769 |
| cloning and sequencing of indian water buffalo (bubalus bubalis) interleukin-3 cdna. | full-length cdna (435 bp) of the interleukin-3(il-3) gene of the indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. this sequence had 96% nucleotide identity and 92% amino acid identity with bovine il-3. there are 10 amino acid substitutions in buffalo compared with that of bovine. the amino acid sequence of buffalo il-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. structural homology modell ... | 2011 | 22152058 |
| occurrence of theileria and babesia species in water buffalo (bubalus babalis, linnaeus, 1758) in the hubei province, south china. | the presence and prevalence of tick-borne haemoparasites in water buffalo from the hubei province, south china was investigated using the reverse line blot (rlb) hybridization assay and phylogenetic analysis of the parasite 18s rrna gene. theileria buffeli (19.1%) was the most frequently found species in all of the locations, followed by babesia orientalis (8.9%), babesia bovis (1.0%) and babesia bigemina (0.7%). only 12 (3.9%) of the samples had mixed infections. eleven samples with single infe ... | 2011 | 22154255 |
| Diversity analysis of methanogens in rumen of Bubalus bubalis by 16S riboprinting and sequence analysis. | The molecular diversity of rumen methanogens was investigated by 16S rDNA gene library prepared from the rumen contents obtained from Murrah buffaloes in India. Genomic DNA was isolated from adult male fistulated buffaloes and PCR conditions were set up using specific primers. Amplified product was cloned into a suitable vector, and the positive clones were selected assuming based on blue-white screening and sequenced. Positive clones were reamplified and the resulting PCR products were further ... | 2012 | 22155312 |
| structure-activity relationship of buffalo antibacterial hepcidin analogs. | hepcidin is an anti-microbial peptide expressed predominantly in the liver of many species. based on the amino acid sequence deduced from buffalo (bubalus bubalis) hepcidin cdna (accession no. eu399814), six peptides hepc(1-25), hepc(6-25), hepc(7-25), hepc(9-25), hepc(11-25) and hepc(15-25) were synthesized using solid-phase fluorenylmethoxycarbonyl (fmoc) chemistry. cd spectroscopy revealed different spectra of the peptides in different solvents and in all the cases beta-structure was found to ... | 2011 | 22165290 |
| Fermentation kinetics of sainfoin hay with and without PEG. | The in vitro gas production technique was applied to evaluate the biological activity of tannins in sainfoin (Onobrychis viciifolia Scop.) hay cut at four progressive phenological stages. The buffalo (Bubalus bubalis) rumen fluid was used as inoculum, polyethylene glycol (PEG) was added as specific binding agent and an alfalfa hay (AH) was used as control. All samples were analysed for chemical composition and condensed tannin (CTs). According to the phenological stage of sainfoin, crude protein ... | 2011 | 22168179 |
| Frequency of Toxoplasmosis in Water Buffalo (Bubalus bubalis) in Trinidad. | Toxoplasmosis has been reported to occur in several animals and humans causing different clinical manifestations. The study was conducted to determine the frequency of Toxoplasma gondii antibodies (IgG) in water buffalo (Bubalus bubalis) across farms in Trinidad using a latex agglutination test. Of a total of 333 water buffalo tested, 26 (7.8%) were seropositive for T. gondii antibodies. Seropositivity for toxoplasmosis was statistically significantly (P < 0.05; ?(2)) higher in adult water buffa ... | 2011 | 22195295 |
| evaluation of blood antioxidant defense and apoptosis in peripheral lymphocytes on exogenous administration of pineal proteins and melatonin in rats. | in view of the significant health impact of oxidative stress and apoptosis dysfunction, and further, because of suggestions that administration of antioxidants might reduce apoptosis rate through up-regulation of body antioxidant defense systems, therefore the purpose of this study was to compare the effect of buffalo (bubalus bubalis) pineal proteins (pp at 100 μg/kg bw, i.p.) with melatonin (mel at 10 mg/kg bw, i.p.) on blood (erythrocytes) antioxidant defense system and apoptosis in isolated ... | 2011 | 22205582 |
| soya-lecithin in extender improves the freezability and fertility of buffalo (bubalus bubalis) bull spermatozoa. | egg yolk is routinely used as a cryoprotectant in semen extenders. however, it may contain cryoprotective antagonists, and there are hygienic risks associated with its use. proteins of plant origin, like soya-lecithin, lack these hazards. the aim of this study was to use soya-lecithin as a cryoprotectant in extender and to investigate its effects on in vitro quality and in vivo fertility of buffalo semen. semen from three buffalo bulls was frozen in tris-citric extender containing 5.0%, 10% or 1 ... | 2011 | 22211278 |
| characterization of prlr and ppargc1a genes in buffalo (bubalus bubalis). | more than 40 million households in india depend at least partially on livestock production. buffaloes are one of the major milk producers in india. the prolactin receptor (prlr) gene and peroxisome proliferators activated receptor-γ coactivator 1-alpha (ppargc1a) gene are reportedly associated with milk protein and milk fat yields in bos taurus. in this study, we sequenced the prlr and ppargc1a genes in the water buffalo bubalus bubalis. the prlr and ppargc1a genes coded for 581 and 819 amino ac ... | 2011 | 22215963 |
| lacking expression of paternally-expressed gene confirms the failure of syngamy after intracytoplasmic sperm injection in swamp buffalo (bubalus bubalis). | the objectives were to: (1) examine the efficiency of intracytoplasmic sperm injection (icsi) technique, with or without chemical activation of in vitro matured buffalo oocytes, on sperm head decondensation; and (2) compare the subsequent development of embryos after activation of icsi (icsi (+) activation group) and sham injection (sham (+) activation group) oocytes (embryos obtained by in vitro fertilization of ivm oocytes served as a control group). pronuclear formation rates in icsi (+) acti ... | 2012 | 22225682 |
| production of nuclear transfer embryos by using somatic cells isolated from milk in buffalo (bubalus bubalis). | somatic cells in milk are a potential source of nuclei for nuclear transfer to produce genetically identical animals; this is especially important in animals that are susceptible to risks of bacterial infection on biopsy collection. in this study, a minimum of 10 milk samples were collected from each of the three buffaloes representing murrah breed. all the samples were processed immediately and cell colonies were obtained. cell colonies from one buffalo (mu-442) survived beyond 10 passages and ... | 2012 | 22229797 |
| pharmacokinetics and relative bioavailability of praziquantel in healthy water buffalo after oral and intramuscular administration. | 2012 | 22239339 | |
| experimental inoculation of neospora caninum in pregnant water buffalo. | the aim of this study was to characterize the pathogenesis of neospora caninum in experimentally inoculated pregnant water buffalo (bubalus bubalis). twelve mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to n. caninum by indirect fluorescent antibody test (ifat) were involved. ten females were intravenously inoculated with 10(8) tachyzoites of nc-1 strain at 70 (n=3) or 90 (n=7) days of pregnancy (dp). two control animals were inoculated with placebo ... | 2011 | 22244534 |
| effects of plane of nutrition on growth, feed intake, digestibility and nitrogen balance in murrah graded male buffalo (bubalus bubalis) calves in nepal. | an experiment was conducted using 17 male buffalo calves to assess the effects of plane of nutrition on dry matter intake (dmi), daily gain (dg), body size measurement, apparent digestibility and nitrogen (n) balance. to attain 250 kg bw, the calves were allocated into three groups: h, l-h and l, receiving the concentrate at 1.50% of bw, 0.75% of bw until 190 kg bw and 1.50% thereafter and 0.75% of bw, respectively. the animals had ad libitum access to urea-treated rice straw (utrs). the dmi of ... | 2012 | 22250739 |
| co-culture of buffalo (bubalus bubalis) preantral follicles with antral follicles: a comparative study of developmental competence of oocytes derived from in vivo developed and in vitro cultured antral follicles. | the present study was undertaken to examine whether the presence of antral follicles (afs) affects the survival, growth and steroidogenesis of preantral follicles (pfs) and compare the maturation and developmental competence of buffalo oocytes derived from in vivo developed and in vitro cultured afs. two experiments were carried out. in experiment i, pfs (200-250 μm) were isolated and cultured with or without afs (3-5 mm) in tcm-199 medium that contained 10% fetal bovine serum (fbs), 1% insulin ... | 2013 | 22251772 |
| alterations in follicular fluid estradiol, progesterone and insulin concentrations during ovarian acyclicity in water buffalo (bubalus bubalis). | ovarian acyclicity is one of the most important causes of infertility in water buffalo. recent studies have indicated alterations in the composition of follicular fluid during the condition. the aim of this study was to determine the changes in follicular fluid concentrations of estradiol, progesterone and insulin during ovarian acyclicity in water buffalo. ovaries were collected from 50 acyclic and 95 cyclic (control) buffaloes and follicular fluid was aspirated from small (5.0-6.9 mm), medium ... | 2012 | 22277844 |
| acp5 (uteroferrin): phylogeny of an ancient and conserved gene expressed in the endometrium of mammals. | type 5 acid phosphatase (acp5; also known as tartrate-resistant acid phosphatase or uteroferrin) is a metalloprotein secreted by the endometrial glandular epithelium of pigs, mares, sheep, and water buffalo. in this paper, we describe the phylogenetic distribution of endometrial expression of acp5 and demonstrate that endometrial expression arose early in evolution (i.e., before divergence of prototherian and therian mammals ~166 million years ago). to determine expression of acp5 in the pregnan ... | 2012 | 22278982 |
| an association analysis between oxt genotype and milk yield and flow in italian mediterranean river buffalo. | the aim of this study was to evaluate possible associations between three snps at the oxytocin locus (am234538: g.28c>t; g.204a>g and g.1627g>t) and two productive traits, milk yield and milkability, in italian mediterranean river buffaloes. effects of parity, calving season and month of production were also evaluated. a total of 41 980 test-day records belonging to 219 lactations of 163 buffalo cows were investigated. the allele call rate was 98·8% and the major allele frequency for all the inv ... | 2012 | 22280971 |
| serological profile of buffalo (bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose bengal, 2-mercaptoethanol and complement fixation tests. | the serological profiles of 21 female buffaloes vaccinated between 3 and 8 months of age using brucella abortus strain 19 (s19) were evaluated by rose bengal (rbt), 2-mercaptoethanol (2me) and complement fixation (cft) tests. the serum strains were collected in day zero, 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. no animal showed reaction in day zero. in 15th day above 95% of animals revealed reaction in all tests. all the animals presented absen ... | 2012 | 22284623 |
| analysis of rumen methanogen diversity in water buffaloes (bubalus bubalis) under three different diets. | the water buffalo (bubalus bubalis) is a prominent livestock species for the production of milk and meat in many countries. we investigated the diversity of rumen methanogens in mediterranean water buffaloes maintained in brazil under different diets: corn silage, grazing pasture, or sugar cane. a total of 467 clones were isolated from three methanogen 16s rrna gene clone libraries that each represented a distinct feed type. the 467 clones were assigned to 19 species-level operational taxonomic ... | 2012 | 22286379 |
| serological and bacteriological responses of water buffalo (bubalus bubalis) vaccinated with two doses of brucella abortus strain rb51 vaccine. | thirty-two water buffalo (bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to brucella abortus strain rb51 (rb51) vaccination in a dose-response study and to compare the use of two selective media for the isolation of rb51. the animals were randomly divided into three treatment groups. groups i-iii received the recommended vaccine dose (rd) twice 4 weeks apart, rd twice 18 weeks apart and saline once, respectively. lymph nodes were excised from the three group ... | 2012 | 22297422 |
| pregnancy-specific protein b (pspb), progesterone and some biochemical attributes concentrations in the fetal fluids and serum and its relationship with fetal and placental characteristics of iraqi riverine buffalo (bubalus bubalis). | this study was carried out to demonstrate the pregnancy-specific protein b (pspb), progesterone and some biochemical parameters concentrations in amniotic fluid, allantoic fluid and fetal serum collected from slaughtered iraqi riverine pregnant buffaloes at three different months of gestation (6th, 7th and 8th). ten out of 22 adult buffaloes of 4.6 ± 0.97 years old were used in this study. the buffaloes were mated naturally by monitoring the estrus cycles via appearance of vaginal fluids and mou ... | 2012 | 22325967 |
| the past and present of and potential for the domestic (water) buffalo in africa. | egypt is the only country in africa where domestic buffalo have a long-term presence and constitute an important part of the array of domestic animal resources. attempts to introduce buffalo to other african countries have been made since at least the 1920s. nine such attempted introductions are documented in this paper, although for most cases, there is very limited information. buffalo have disappeared without trace in some countries and were slaughtered in at least two because of lack of adap ... | 2012 | 22327409 |
| microbial population in the rumen of swamp buffalo (bubalus bubalis) as influenced by coconut oil and mangosteen peel supplementation. | four, rumen fistulated swamp buffalo bulls were used to study microbial populations in the rumen when supplemented with coconut oil and mangosteen peel. animals were randomly assigned to a 4 × 4 latin square design. four treatments were un-supplemented (control), supplementation with coconut oil at 50 g/kg (co5), supplementation with mangosteen peel at 30 g/kg (mp3) and supplementation with co5 and mp3 (com), of total dm intake. animals received concentrate at 10 g/kg of bw, and rice straw was g ... | 2013 | 22356106 |
| phenotype characterization of staphylococcus species strains isolated from buffalo (bubalus bubalis) milk. | the objective of the current study was to isolate and identify phenotypes of staphylococcus spp. strains derived from buffalo (bubalus bubalis) milk. a total of 548 milk samples from 137 buffalo were cultured in columbia agar enriched with 5% defibrinated sheep blood. determination of the capacity of staphylococcus aureus to produce enterotoxins a-d and toxic shock syndrome toxin-1 (tsst-1) was achieved by reverse passive latex agglutination (rpla). antimicrobial sensitivity of s. aureus strains ... | 2011 | 22362803 |
| effect of incorporation of additives in tris-based egg yolk extender on buffalo (bubalus bubalis) sperm tyrosine phosphorylation during cryopreservation. | phosphorylation of tyrosine residues on sperm protein is a known indicator of capacitation and a major intracellular signalling event. there is evidence that sperm cryopreservation promotes tyrosine phosphorylation and is associated with reduced fertility of spermatozoa. under this study, cryoprotective role of different additives namely taurine, trehalose, catalase and 4-bromophenacyl bromide on buffalo sperm quality was evaluated. buffalo semen was cryopreserved in tris-based egg yolk extender ... | 2011 | 22364363 |
| short communication: prevalence and risk factors of subclinical mastitis as determined by the california mastitis test in water buffaloes (bubalis bubalis) in nueva ecija, philippines. | a retrospective analysis using records of lactating bulgarian murrah buffaloes subjected to the california mastitis test in a herd in nueva ecija, philippines was done to determine the prevalence of subclinical mastitis (scm) and to identify risk factors that may influence its occurrence and recurrence. results showed that scm prevalence was 42.76%, whereas its recurrence was 75.03%. age and lactation length influenced the occurrence of scm. in contrast to the conclusions for dairy cows, younger ... | 2012 | 22365218 |
| roscovitine treatment improves synchronization of donor cell cycle in g0/g1 stage and in vitro development of handmade cloned buffalo (bubalus bubalis) embryos. | this study investigated the effects of serum-starvation, total confluence, and roscovitine treatment on cell-cycle synchronization of buffalo ear skin fibroblasts to the g0/g1 stage and on the developmental competence of cloned embryos. serum starvation of total confluence cultures for 24 h had a higher (p<0.05) proportion of cells at g0/g1 stage (94.4%) compared with serum starved cyclic and nonstarved confluent cultures (76.8 and 86.0%, respectively), whereas differences between cyclic cells w ... | 2012 | 22372577 |
| effect of bypass fat supplementation on productive performance and blood biochemical profile in lactating murrah (bubalus bubalis) buffaloes. | the study investigated the effect of dietary supplementation of bypass fat on productive performance and blood biochemical profile of lactating murrah buffaloes (bubalus bubalis). fifteen multiparous buffaloes (2-4 lactation) of early to mid lactation were divided in three homogenous groups t(1) (control), t(2), and t(3) of five each. the animals in t(1) were fed with a basal diet consisting of a concentrate mixture, green sorghum, and wheat straw as per requirements, while the animals in group ... | 2012 | 22373930 |
| animal production systems of small farms in the kaski district of nepal. | the aim of this study was to describe animal production on small farms in the kaski district of nepal, with the goal of identifying areas where animal health and productivity could be improved. eighty-five randomly selected farms from four different village development committees were visited. farmers were interviewed and premises and animals visually inspected on all farms. feed samples were collected from a subset of farms. the most commonly kept species were water buffalo (used for milk and m ... | 2012 | 22392548 |
| pcr-sscp analysis of leptin gene and its association with milk production traits in river buffalo (bubalus bubalis). | leptin gene has been found to be associated with various economic traits including milk production and fat quality in dairy animals. in the present study, we investigated genetic variations in intron 1 region of leptin gene in riverine buffaloes (bubalus bubalis) using polymerase chain reaction-single strand conformation polymorphism (pcr-sscp) and sequencing methods and associated them with milk traits. the study revealed three sscp variants a, b and c among a total of 301 buffaloes from nine b ... | 2012 | 22395794 |
| inferring the phylogeny of bovidae using mitochondrial dna sequences: resolving power of individual genes relative to complete genomes. | molecular techniques that assess biodiversity through the analysis of a small segment of mitochondrial genome have been getting wide attention for inferring the mammalian diversity. due to their highly conserved nature, specific mitochondrial genes offer a promising tool for phylogenetic analysis. however, there is no established criteria for selecting the typical mitochondrial dna (mtdna) segments to achieve a greater resolving power. we therefore chose the family bovidae as a model and compare ... | 2012 | 22399841 |
| improved rapd-pcr for discriminating breeds of water buffalo. | 2012 | 22403004 | |
| comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo. | yellow cattle and water buffalo are two of the most important natural hosts for schistosoma japonicum in china. previous observation has revealed that yellow cattle are more suited to the development of s. japonicum than water buffalo. understanding more about the molecular mechanisms involved in worm development, as well as the pathological and immunological differences between yellow cattle and water buffalo post infection with s japonicum will provide useful information for the vaccine design ... | 2012 | 22414188 |
| chromosome fragility in river buffalo cows exposed to dioxins. | fifty river buffalo (bubalus bubalis, 2n = 50) cows reared in two different provinces of campania (southern italy) underwent cytogenetic investigations to ascertain possible differences in their chromosome stability. one group (caserta province) was under legal sequestration due to the presence in the milk mass of higher mean values of dioxins [21.79 pg/g of fat as sum of polychloro-dibenzo-dioxins (pcdds), polychloro-dibenzo-furans (pcdfs) and dioxin-like polychlorobiphenyls (dl-pcbs)] than bot ... | 2012 | 22415351 |
| generation of induced pluripotent stem cells from buffalo (bubalus bubalis) fetal fibroblasts with buffalo defined factors. | ectopically, expression of defined factors could reprogram mammalian somatic cells into induced pluripotent stem cells (ipscs), which initiates a new strategy to obtain pluripotent stem cell lines. attempts have been made to generate buffalo pluripotent stem cells by culturing primary germ cells or inner cell mass, but the efficiency is extremely low. here, we report a successful method to reprogram buffalo fetal fibroblasts (bffs) into pluripotent stem cells [buffalo induced pluripotent stem ce ... | 2012 | 22420535 |
| the endocrine changes, the timing of ovulation and the efficacy of the doublesynch protocol in the murrah buffalo (bubalus bubalis). | experiments were conducted to investigate (a) the timing of ovulation and the associated endocrine changes (progesterone, estrogen and lh) during estrous cycle and (b) the efficacy, with respect to the pregnancy rate, in cycling and anestrus in murrah buffaloes subjected to the doublesynch protocol during the low breeding season. in experiment 1, 10 cycling buffaloes were administered pgf(2α) on day 0 (without regard to the estrous cycle stage), gnrh on day 2, a second pgf(2α) injection on day 9 ... | 2012 | 22433942 |
| characterization of osteopontin gene of bubalus bubalis. | osteopontin, a glycoprotein, is expressed in several tissues including the mammary gland. the gene has been reported to be associated with milk and its constituents in various livestock species. this gene was sequenced in buffalo and it coded for the protein of 280 amino acids with the conserved grgds domain. the sequence was confirmed from the cdna sequence derived from the mammary gland of buffalo. the earlier-reported 9t/10t variation in the upstream region of the gene was investigated for it ... | 2008 | 22443697 |
| α-tocopherol and l-ascorbic acid increase the in vitro development of ivm/ivf swamp buffalo ( bubalus bubalis) embryos. | this study was conducted to investigate the effects of capacitating agents added at in vitro fertilization (ivf) and antioxidants supplemented during in vitro culture (ivc) on the development of buffalo embryos. in experiment i, in vitro embryo development of buffalo embryos was compared when the ivf medium was supplemented with heparin, caffeine and calcium ionophore a23187 either alone or in combination. there was no significant difference (p > 0.05) in the cleavage rates of oocytes among the ... | 2008 | 22443906 |
| effect of bromocryptine on hormones and milk secretion in murrah buffaloes ( bubalus bubalis). | an experiment was carried out on 10 advance pregnant murrah buffaloes to determine the role of hormones in milk secretion around parturition. experimental animals were administered with a single injection of bromocryptine, @ 100 μg/kg bw, for 5 days before expected calving, whereas control group buffaloes were injected with placebo injections. blood samples collected before parturition (-5,-4,-3,-2,-1 days), on day of parturition (day-0) and on day 1, 2, 3, 4, 5, 10 and 15 post partum were analy ... | 2010 | 22444132 |
| identification and validation of reference genes for gene expression studies in water buffalo. | in gene expression analysis, a key step to obtain informative data from reverse transcription quantitative pcr (rt qpcr) assay is normalization, that is usually achieved by ratio to correct the abundance of the gene of interest against that of an endogenous reference gene. the finding of such reference genes, ideally expressed in a stable way in multiple tissue samples and in different experimental conditions, is a non-trivial problem. in this work, a set of genes potentially useful as reference ... | 2010 | 22444257 |
| effect of spermine-nonoate on acrosome reaction and associated protein tyrosine phosphorylation in murrah buffalo (bubalus bubalis) spermatozoa. | the present study was conducted to know the role of nitric oxide (no) on the acrosome reaction (ar) in murrah buffalo (bubalus bubalis) spermatozoa. ejaculated buffalo spermatozoa were washed, suspended in sp-talp media containing 6 mg bsa/ml and cell concentration was adjusted to 50×10(6) cells/ml. the cells were incubated for 6h in the absence or presence of heparin (10 μg/ml) to induce capacitation. fully capacitated spermatozoa were incubated in presence of 100 μg/ml lysophosphatidyl choline ... | 2012 | 22445612 |
| influence of intergeneric/interspecies mitochondrial injection; parthenogenetic development of bovine oocytes after injection of mitochondria derived from somatic cells. | interspecies/intergeneric mitochondrial heteroplasmy can occur in interspecies/intergeneric hybrid embryos or following nuclear transfer. in the present study, intergeneric buffalo (bubalus bubalis) mitochondria (wb-mt) or interspecies murine (mus spretus) mitochondria (m-mt) were injected into bovine (bos taurus) oocytes, and the subsequent embryonic development was characterized. fibroblast mitochondria (wb-mt or m-mt) were microinjected into in vitro matured bovine oocytes followed by oocyte ... | 2012 | 22447326 |
| oxygen concentration and cysteamine supplementation during in vitro production of buffalo (bubalus bubalis) embryos affect mrna expression of bcl-2, bcl-xl, mcl-1, bax and bid. | this study examined the effects of o(2) concentration (5% vs 20%) during in vitro maturation (ivm), fertilization (ivf) and culture (ivc) or supplementation of ivm and ivc media with cysteamine (50 and 100 μm, respectively; ivm, ivf and ivc carried out in 20% o(2)), on blastocyst rate and relative mrna abundance of some apoptosis-related genes measured by real-time qpcr in immature and in vitro-matured buffalo oocytes and in embryos at 2-, 4-, 8- to 16-cell, morula and blastocyst stages. the bla ... | 2012 | 22452597 |
| embryonic genome activation events in buffalo (bubalus bubalis) preimplantation embryos. | embryonic genome activation (ega) is the first major step towards successful initiation of preimplantation development, which culminates in the formation of implantation-competent embryos. ega occurs at species-specific embryonic cell stages. in the present work, ega was identified for buffalo embryos by studying the development rate of embryos in normal as well as imposed transcription block conditions, analyzing bromo-uridine triphosphate (brutp) incorporation rates as evidence of de novo tran ... | 2012 | 22461405 |
| expression and characterization of constitutive heat shock protein 70.1 (hspa-1a) gene in in vitro produced and in vivo-derived buffalo (bubalus bubalis) embryos. | cells are blessed with a group of stress protector molecules known as heat shock proteins (hsps), amongst them hsp70, encoded by hspa-1a gene, is most abundant and highly conserved protein. variety of stresses hampers the developmental competence of embryos under in vivo and in vitro conditions. present work was designed to study the quantitative expression of hspa-1a mrna in immature oocytes (imo), matured oocytes (mo), in vitro produced (ivp) and in vivo-derived (ivd) buffalo embryos to assess ... | 2012 | 22463675 |
| effect of l-arginine methyl ester (l-name) on hormonal profile and estrous cycle length in buffaloes (bubalus bubalis). | the present study was undertaken to evaluate the effect of l-arginine methyl ester (l-name), a nitric oxide synthase inhibitor on serum nitric oxide, progesterone, estradiol profiles and estrous cycle length in buffaloes. murrah buffaloes (n = 16) exhibiting regular estrous cycles were randomly allocated to two groups of eight animals. in the treatment group, buffaloes were administered 400 mg/h l-name over 2 h (total dose = 800 mg) via the coccygeal artery and the aorta abdominalis on day 15 of ... | 2012 | 22476734 |
| molecular characterization of mhc-drb cdna in water buffalo (bubalus bubalis). | in the present study, water buffalo mhc (bubu)-drb cdna was cloned and characterized. the 1022 base long-amplified cdna product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. the bubu-drb sequence showed maximum homology with the bola-drb3*0101 allele of cattle. a total of seven amino acid residues were found to be unique for the bubu-drb sequence. the majority of amino acid substitutions was observed in the β(1) domain. residues associated with important fu ... | 2012 | 22481880 |
| mitochondrial dna diversity patterns in pakistani buffalo. | the indian subcontinent is considered to be the likely centre of river buffalo domestication, based on population dynamics, archaeological evidence and genetic diversity. recent studies on mitochondrial dna diversity have drawn useful conclusions about the domestication history of bubalus bubalis. the conclusions of these studies are, however, incomplete, unless samples can also be analysed from pakistan, which contains the second largest buffalo population of the world. here, we report the resu ... | 2011 | 22486503 |
| expression of transcriptional factor genes (oct-4, nanog, and sox-2) and embryonic stem cell-like characters in placental membrane of buffalo (bubalus bubalis). | the aim of the study was to assess the expression of transcriptional factor genes and embryonic stem cell-like characters in the placental membrane of buffalo (bubalus bubalis). along with the placenta, amniotic fluid, maternal peripheral blood, and umbilical cord blood samples were taken for the future study. the isolation and culture of cells from the placental membrane was followed by the determination of rt-pcr-based markers (oct-4, nanog, sox-2, alkaline phosphatase, stem cell factor, and n ... | 2012 | 22527603 |
| clinical and pathological insights into johne's disease in buffaloes. | alternative diagnostic tools and interesting epidemiological assumptions were associated with an outbreak of johne's disease. in a buffalo herd infected with paratuberculosis, seven clinically affected animals and 21 animals with anti-mycobacterium avium elisa reactions were identified. total herd included 203 buffaloes. most lesions were comparable to those described in buffaloes and cattle affected by johne's disease. water buffalo behaviors such as communal nursing and allosuckling may be add ... | 2012 | 22528538 |
| population structure and genetic variability in the murrah dairy breed of water buffalo in brazil accessed via pedigree analysis. | the objective of this study was to use pedigree analysis to evaluate the population structure and genetic variability in the murrah dairy breed of water buffalo (bubalus bubalis) in brazil. pedigree analysis was performed on 5,061 animals born between 1972 and 2002. the effective number of founders (fe) was 60, representing 6.32 % of the potential number of founders. the effective number of ancestors (fa) was 36 and the genetic contribution of the 17 most influent ancestors explained 50 % of the ... | 2012 | 22535150 |
| spermatogonial stem cells (sscs) in buffalo (bubalus bubalis) testis. | water buffalo is an economically important livestock species and about half of its total world population exists in india. development of stem cell technology in buffalo can find application in targeted genetic modification of this species. testis has emerged as a source of pluripotent stem cells in mice and human; however, not much information is available in buffalo. | 2012 | 22536454 |
| polymorphism and haplotype structure in river buffalo (bubalus bubalis) toll-like receptor 5 (tlr5) coding sequence. | most of the 160 million river buffalo in the world are in asia where they are used extensively, both as a food source and for draught power. only recently have investigations begun exploring the buffalo genome for variation that might influence health and productivity of these economically important animals. this paper describes the sequence variability of the toll-like receptor 5 (tlr5) gene, which recognizes bacterial flagellin and is a key player in the immune system. tlr5 is comprised of a s ... | 2012 | 22537062 |
| effect of post-fusion holding time, orientation and position of somatic cell-cytoplasts during electrofusion on the development of handmade cloned embryos in buffalo (bubalus bubalis). | the present study was conducted primarily to optimize electrofusion conditions for efficient production of zona-free nuclear transfer embryos in buffalos (bubalus bubalis). we found that 4v ac current for proper triplet alignment and single step fusion method, using a single dc pulse of 3.36 kv/cm for 4-μs duration, produced the most convincing results for efficient reconstitution of zona-free cloned embryos. lysis rate was very high (84.28 ± 2.59%) when triplets were in physical contact with ne ... | 2012 | 22541327 |
| milk progesterone enzyme-linked immunosorbent assay as a tool to investigate ovarian cyclicity of water buffaloes in relation to body condition score and milk production. | application of assisted reproductive technologies in buffaloes is limited to some extent by farmers' inability to detect oestrus because of its poor expression. the present study aimed at investigating reliability of a milk progesterone enzyme-linked immunosorbent assay (elisa) to assess the ovarian cyclicity during post partum, oestrus and post-breeding periods in water buffaloes. | 2012 | 22554129 |
| power of exclusion of 19 microsatellite markers for parentage testing in river buffalo (bubalus bubalis). | in the present study, 19 microsatellite markers were assessed for their power of exclusion to test parentage in river buffalo. microsatellite genotypes of 216 unrelated buffaloes belonging to five different breeds were utilized for the study. the probabilities of exclusion were calculated for three hypothetical situations viz. paternity testing (pe1), one parental genotype unavailable (pe2) and exclusion of both parents i.e. substituted offspring (pe3). the mean probability of exclusion across 1 ... | 2012 | 22555978 |
| characterization and in silico analysis of pregnancy-associated glycoprotein-1 gene of buffalo (bubalus bubalis). | pregnancy-associated glycoproteins (pags) are trophoblastic proteins belonging to the aspartic proteinase family secreted by different placental cells of many mammalian species. they play a pivotal role in placentogenesis, foetomaternal unit remodeling, and implantation. the identification of the genes encoding those proteins will be helpful to unravel the intricate embryogenomic functions during pregnancy establishment. considering importance of these proteins, the present study was undertaken ... | 2011 | 22567354 |
| effects of plane of nutrition on slaughtering traits and meat characteristics in murrah graded male buffalo (bubalus bubalis) calves in nepal. | an experiment was conducted using 17 male buffalo calves to assess the effects of plane of nutrition on slaughtering traits and meat characteristics. to attain 250 kg body weight (bw), the calves were allocated into three groups: high (h), low-high (l-h) and low (l) corresponding to concentrate levels receiving the concentrate at 1.50% of bw, 0.75% of bw until 190 kg bw and 1.50% thereafter, and 0.75% of bw, respectively. the animals had ad libitum access to urea-treated rice straw. no significa ... | 2011 | 22574796 |
| equivalency of buffalo (bubalus bubalis) embryonic stem cells derived from fertilized, parthenogenetic, and hand-made cloned embryos. | this study was aimed at establishing buffalo embryonic stem cells (escs) from in vitro fertilized (ivf), parthenogenetic, and hand-made cloned (hmc) embryos and to check their equivalency in terms of stem cell marker expression, longevity, proliferation, and differentiation pattern. escs derived from all three sources were found by immunofluorescence to express the pluripotency markers ssea-4, tra-1-60, tra-1-81, oct4, and sox2 and were able to form embryoid bodies containing cells expressing ge ... | 2012 | 22582863 |
| uterine infection influences size and follicular fluid composition of the largest follicle in buffalo (bubalus bubalis). | the effect of uterine infection on size and follicular fluid composition of the largest follicle was studied in buffalo. reproductive tracts were collected from 102 graded murrah buffaloes at an abattoir. uterine infection was diagnosed by physical examination of uterine mucus, white side test and uterine cytology. samples with pus-containing mucus, positive reaction on white side test and/or >5% neutrophils were considered to be positive for uterine infection. diameter of the largest follicle w ... | 2013 | 22583389 |
| gonadotropin-releasing hormone 1 directly affects corpora lutea lifespan in mediterranean buffalo (bubalus bubalis) during diestrus: presence and in vitro effects on enzymatic and hormonal activities. | the expression of gonadotropin-releasing hormone (gnrh) receptor (gnrhr) and the direct role of gnrh1 on corpora lutea function were studied in mediterranean buffalo during diestrus. immunohistochemistry evidenced at early, mid, and late luteal stages the presence of gnrhr only in large luteal cells and gnrh1 in both small and large luteal cells. real-time pcr revealed gnrhr and gnrh1 mrna at the three luteal stages, with lowest values in late corpora lutea. in vitro corpora lutea progesterone p ... | 2012 | 22592497 |
| effect of csn1s1-csn3 (α(s1)-κ-casein) composite genotype on milk production traits and milk coagulation properties in mediterranean water buffalo. | the aim of this study was to estimate effects of csn1s1-csn3 (α(s1)-κ-casein) composite genotypes on milk production traits and milk coagulation properties (mcp) in mediterranean water buffalo. genotypes at csn1s1 and csn3 and coagulation properties [rennet clotting time (rct), curd firming time (k₂₀), and curd firmness (a₃₀)] were assessed by reversed-phase hplc and computerized renneting meter analysis, respectively, using single test-day milk samples of 536 animals. alternative protein varian ... | 2012 | 22612978 |
| analysis of in vitro effects of sex steroids on lymphocyte responsiveness in murrah buffaloes (bubalus bubalis). | present study was carried out on forty four apparently healthy murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro. estrogen inhibited proliferation in mitogen-stimulated lymphocytes from prepubertal but not post pubertal buffaloes of either sex. estrogen at 100 pg/ml concentration stimulating the proliferation significantly (p < 0.05). in all groups and had higher stimulatory effect in lymphocytes from day 10 than ... | 2012 | 22619742 |
| sequence and topological characterization of toll-like receptor 8 gene of indian riverine buffalo (bubalus bubalis). | in this study, buffalo (bubalus bubalis) toll-like receptor 8 (tlr8) gene has been characterized by sequence analysis and detecting polymorphism. complete orf of buffalo tlr8 gene was amplified using the rna isolated from spleen tissue, which was found to be 3,102 nucleotides long encoding a 1,033 amino acid protein. buffalo tlr8 had 10 nucleotide changes as compared to other livestock species resulting in six unique amino acid changes, four of them lying within leucine-rich repeat (lrr) domains ... | 2013 | 22622670 |
| can rare positive interactions become common when large carnivores consume livestock? | livestock populations in protected areas are viewed negatively because of their interaction with native ungulates through direct competition for food resources. however, livestock and native prey can also interact indirectly through their shared predator. indirect interactions between two prey species occur when one prey modifies either the functional or numerical responses of a shared predator. this interaction is often manifested as negative effects (apparent competition) on one or both prey s ... | 2012 | 22624309 |
| construction and preliminary characterization of a river buffalo bacterial artificial chromosome library. | river buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of bubalus bubalis will be available shortly. nonetheless, large-insert dna library resources such as bacterial artificial chromosomes (bac) are still required for validation and accurate assembly of the genome sequence. we constructed a river buffalo bac library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 × coverage of the genome. this genomic resource for ri ... | 2012 | 22653673 |
| natural trematode infection in liver of water buffalo (bubalus bubalis): histopathological investigation. | this study reports the infection of liver and bile ducts, carried out from november 2008 to april 2010, on 730 randomly selected water buffaloes-bubalusbubalis, infected with the amphistome trematode parasite explanatum explanatum (creplin, 1847) fukui, 1929. macroscopic examination revealed massive infection of adult fluke in bile ducts and intrahepatic ductules in 131 (18%) cases. the predominant features were multifocal granulomatous nodules throughout the luminal surface of the bile ducts. h ... | 2011 | 22654314 |
| advanced comparative cytogenetic analysis of x chromosomes in river buffalo, cattle, sheep, and human. | based on a recently generated comprehensive gene map for ovis aries chromosome x (oarx) with an approximately even locus distribution, we assigned selected bacterial artificial chromosome (bac) probes corresponding to these oarx loci to bubalus bubalis (bbu) and bos taurus (bta) by comparative fluorescence in-situ hybridization (fish) to improve cytogenetically the x chromosome maps in these species. twenty-five added loci in bbux and btax, respectively, contribute to a more detailed description ... | 2012 | 22669522 |
| the effects of high temperature and roof modification on physiological responses of swamp buffalo (bubalus bubalis) in the tropics. | the objective of the experiments reported here was to measure the effects of cooling techniques (modified roof vs normal roof) on the performance and physiology of 12 young male buffaloes with a similar live weight of 160 kg. the study was conducted at chainat agriculture and technology college, chainat province, thailand. the animals were divided randomly into two groups, each group comprising six buffaloes, and the two groups were studied to evaluate the effects of modified roofing (normal roo ... | 2013 | 22678302 |
| in vivo differentiation potential of buffalo (bubalus bubalis) embryonic stem cell. | embryonic stem cells (escs) derived from inner cell mass (icm) of mammalian blastocyst are having indefinite proliferation and differentiation capability for any type of cell lineages. in the present study, icms of in vitro-derived buffalo blastocysts were cultured into two different culture systems using buffalo fetal fibroblast as somatic cell support and matrigel as synthetic support to obtain pluripotent buffalo embryonic stem cell (buesc) colonies. pluripotency of the escs were characterise ... | 2012 | 22678753 |
| sequence characterization of river buffalo toll-like receptor genes 1-10 reveals distinct relationship with cattle and sheep. | the present study was undertaken to characterize the full-length transcripts of toll-like receptor (tlr) genes 1-10 of river buffalo. the conceptualized amino acid identity of bubaline tlrs ranged between 86% to 100% with ruminants, while it ranged between 45% to 91% with other vertebrate species. simple modular architecture tool (smart) analysis revealed the presence of tir domains and varying numbers of leucine-rich repeat motifs in all the buffalo tlrs. with respect to tir domains, tlrs 1, 2 ... | 2013 | 22694123 |