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optimization of culture conditions for exopolysaccharide production by a probiotic strain of lactobacillus rhamnosus e/n.the effects of culture conditions on exopolysaccharides (eps) production by a probiotic lb. rhamnosus e/n have been studied using the plackett-burman design. process optimization was performed in stationary cultures to maximize the production of eps. in order to verify the optimal conditions, an analysis was performed of eps production in fermentation culture. batch fermentation was carried out at working volume of 2.51. the optimal temperature, ph, carbon source, and nitrogen source conditions ...201425115122
severe oral infection due to lactobacillus rhamnosus during induction chemotherapy for acute myeloid leukemia.we report a case of severe oral infection with a high fever due to lactobacillus rhamnosus during induction chemotherapy for acute myeloid leukemia. the patient did not improve on treatment with meropenem, clindamycin, or vancomycin until neutrophil recovery. since l. rhamnosus gg is used in dairy products, and the patient ingested dairy products daily before starting chemotherapy, we suspected an association between the ingestion of dairy products and the development of infection. pulsed-field ...201425115834
antimicrobial and antibiofilm potential of biosurfactants isolated from lactobacilli against multi-drug-resistant pathogens.biosurfactants (bs) are amphiphilic compounds produced by microbes, either on the cell surface or secreted extracellularly. bs exhibit strong antimicrobial and anti-adhesive properties, making them good candidates for applications used to combat infections. in this study, our goal was to assess the in vitro antimicrobial, anti-adhesive and anti-biofilm abilities of bs produced by lactobacillus jensenii and lactobacillus rhamnosus against clinical multidrug resistant (mdr) strains of acinetobacte ...201425124936
study of the lactobacillus rhamnosus lcr35® properties after compression and proposition of a model to predict tablet stability.the beneficial effects of probiotic bacteria on human health are now widely acknowledged, and this has prompted growing interest in research and development in the pharmaceutical field. however, to be viable when they reach their target, the bacteria must be able to survive during the manufacturing process and the biological pathway. tablet form best meets the requirements for protecting acid labile drugs, but the tableting process could be an additional stress for the bacteria. this study evalu ...201425128853
a multispecies probiotic reduces oral candida colonization in denture wearers.the prevalence of candida infections has been rising with an increasingly aging population and a larger population of immunocompromised individuals. the use of probiotics may be an alternative approach to antifungal agents in the prevention and treatment of oral candidiasis. this study aimed to evaluate the short-term effect of probiotics in reducing the infection level of oral candida in candidiasis-asymptomatic elderly denture wearers.201525143068
differential modification of genetic susceptibility to childhood eczema by two probiotics.in a double-blind, randomized, placebo-controlled birth cohort, we have recently shown a beneficial effect of lactobacillus rhamnosus hn001 (hn001) for the prevention of eczema in children through to 6 years of age but no effect of bifidobacterium animalis subsp lactis hn019 (hn019).201425146491
a new risk factor for neonatal vancomycin-resistant enterococcus colonisation: bacterial probiotics.vancomycin-resistant enterococcus (vre) colonisation can be controlled with strict adherence to infection control measures. we describe a vre outbreak coincident with bacterial probiotic trial. relationship between probiotic and vre colonisation, and other possible risk factors were investigated.201525163639
new lactic acid bacterial strains from traditional mongolian fermented milk products have altered adhesion to porcine gastric mucin depending on the carbon source.attachment of lactic acid bacteria to the mucosal surface of the gastrointestinal tract is a major property of probiotics. here, we examined the ability of 21 lactic acid bacterial strains isolated from traditional fermented milk products in mongolia to adhere to porcine gastric mucin in vitro. higher attachment was observed with lactobacillus delbrueckii subsp. bulgaricus strains 6-8 and 8-1 than with lactobacillus rhamnosus gg (positive control). lactococcus lactis subsp. cremoris strain 7-1 a ...201525186082
towards a better understanding of lactobacillus rhamnosus gg--host interactions.lactobacillus rhamnosus gg (lgg) is one of the most widely used probiotic strains. various health effects are well documented including the prevention and treatment of gastro-intestinal infections and diarrhea, and stimulation of immune responses that promote vaccination or even prevent certain allergic symptoms. however, not all intervention studies could show a clinical benefit and even for the same conditions, the results are not univocal. clearly, the host phenotype governed by age, genetics ...201425186587
genomic characterization of non-mucus-adherent derivatives of lactobacillus rhamnosus gg reveals genes affecting pilus biogenesis.lactobacillus rhamnosus gg is one of the best-characterized lactic acid bacteria and can be considered a probiotic paradigm. comparative and functional genome analysis showed that l. rhamnosus gg harbors a genomic island including the spacba-srtc1 gene cluster, encoding the cell surface-decorating host-interacting pili. here, induced mutagenesis was used to study pilus biogenesis in l. rhamnosus gg. a combination of two powerful approaches, mutation selection and next-generation sequencing, was ...201425192985
phenotypic surface properties (aggregation, adhesion and biofilm formation) and presence of related genes in beneficial vaginal lactobacilli.to evaluate the phenotypic expression of auto-aggregation, adhesion to mucin and biofilm formation of lactobacilli isolated from human vagina and the presence of related genes.201425195810
draft genome sequences and description of lactobacillus rhamnosus strains l31, l34, and l35.lactobacillus rhamnosus is a facultative, lactic acid bacterium in the phylum firmicutes. lactobacillus spp. are generally considered beneficial, and specific strains of l. rhamnosus are validated probiotics. we describe the draft genomes of three l. rhamnosus strains (l31, l34, and l35) isolated from the feces of thai breastfed infants, which exhibit anti-inflammatory properties in vitro. the three genomes range between 2.8 - 2.9 mb, and contain approximately 2,700 protein coding genes.201425197459
the efficacy of ampicillin and lactobacillus casei rhamnosus in the active management of preterm premature rupture of membranes remote from term.we aimed to investigate the treatment efficacy of ampicillin prophylaxis accompanied by lactobacillus casei rhamnosus over the latency period following preterm premature rupture of membranes (pprom).201425210439
endogenous biotin-binding proteins: an overlooked factor causing false positives in streptavidin-based protein detection.biotinylation is widely used in dna, rna and protein probing assays as this molecule has generally no impact on the biological activity of its substrate. during the streptavidin-based detection of glycoproteins in lactobacillus rhamnosus gg with biotinylated lectin probes, a strong positive band of approximately 125 kda was observed, present in different cellular fractions. this potential glycoprotein reacted heavily with concanavalin a (cona), a lectin that specifically binds glucose and mannos ...201525211245
tolerability of a probiotic in subjects with a history of methicillin-resistant staphylococcus aureus colonisation.methicillin-resistant staphylococcus aureus (mrsa) is a pathogen of major public health importance. colonisation precedes infection; thus reducing mrsa carriage may be of benefit for reducing infection. probiotics represent a novel approach to reducing mrsa carriage. we undertook a pilot feasibility randomised controlled trial of the tolerability and acceptability of probiotics for reducing nasal and intestinal carriage of mrsa. in addition, subjects were screened for vancomycin-resistant entero ...201425213147
the brain-gut axis: a target for treating stress-related disorders.the brain-gut axis provides a bidirectional means of communication between the microbiota within the gut and the brain. stress acting via the brain can result in alteration of the microbial composition of the gut, but increasing evidence indicates that bacteria within the gut can influence brain neurochemistry and behaviour. it is clear that post-natal colonisation of the gut plays a key role in regulating the development of the hypothalamic-pituitary-adrenal axis and the development of pivotal ...201325224893
short-term probiotic therapy alleviates small intestinal bacterial overgrowth, but does not improve intestinal permeability in chronic liver disease.: although numerous animal studies suggest that probiotic therapy has beneficial effects in various liver diseases, the evidence for beneficial effects in human liver disease is controversial. this study was carried out to investigate the efficacy of probiotic therapy in alleviating small intestinal bacterial overgrowth (sibo) and permeability in chronic liver disease.201425244414
decolonization of gastrointestinal carriage of vancomycin-resistant enterococcus faecium: case series and review of literature.prolonged asymptomatic carriage of vancomycin-resistant enterococci (vre) in the gastrointestinal tract and the lack of effective decolonization regimen perpetuate the endemicity of vre in the healthcare settings.201425248287
the potential hazards of aspergillus sp. in foods and feeds, and the role of biological treatment: a review.the contamination of food and feed by aspergillus has become a global issue with a significant worldwide economic impact. the growth of aspergillus is unfavourable to the development of food and feed industries, where the problems happen mostly due to the presence of mycotoxins, which is a toxic metabolite secreted by most aspergillus groups. moreover, fungi can produce spores that cause diseases, such as allergies and asthma, especially to human beings. high temperature, high moisture, retarded ...201425269603
probiotic characteristics of lactobacillus fermentum strains isolated from tulum cheese.the aim of this study was to characterize the probiotic characteristics of lactobacillus fermentum strains isolated from tulum cheese. seven l. fermentum strains were selected among the isolated and identified lactobacillus strains due to their abundance. when the gastric condition was considered, l. fermentum lp3 and lp4 were able to tolerate ph 2.5 and 1% bile salt. all l. fermentum strains had similar enzymatic activity and antibiotic resistance pattern but the highest antagonistic effect was ...201425270832
eruca sativa might influence the growth, survival under simulated gastrointestinal conditions and some biological features of lactobacillus acidophilus, lactobacillus plantarum and lactobacillus rhamnosus strains.the growth and viability of three lactobacillus strains, lactobacillus acidophilus, lactobacillus plantarum and lactobacillus rhamnosus, after their passage through simulated gastric and pancreatic juices were studied as a function of their presence in the growth medium of rocket salad (eruca sativa). the presence of e. sativa affected some of the biological properties of the strains. for example, l. acidophilus and l. plantarum worked more efficiently in the presence of e. sativa, increasing no ...201425275269
influence of manufacturing processes on cell surface properties of probiotic strain lactobacillus rhamnosus lcr35®.the influence of the industrial process on the properties of probiotics, administered as complex manufactured products, has been poorly investigated. in the present study, we comparatively assessed the cell wall characteristics of the probiotic strain lactobacillus rhamnosus lcr35® together with three of its commercial formulations with intestinal applications. putative secreted and transmembrane-protein-encoding genes were initially searched in silico in the genome of l. rhamnosus lcr35®. a tot ...201525280746
effect of the bread-making process on zearalenone levels.the effects of the bread-making process including fermentation with saccharomyces cerevisiae and lactic acid bacteria (lactobacillus casei, lactobacillus rhamnosus, lactobacillus acidophilus and lactobacillus fermentum) and baking at 200°c on zearalenone (zea) levels were investigated. standard solutions of zea were added to flour and then loaves of bread were prepared. sourdough and three types of yeast including active dry yeast, instant dry yeast and compressed yeast were used for the ferment ...201425291600
randomized open-label pilot study of the influence of probiotics and the gut microbiome on toxic metal levels in tanzanian pregnant women and school children.exposure to environmental toxins is a 21st century global health problem that is often the result of dietary intake. although efforts are made to reduce dietary toxin levels, they are often unsuccessful, warranting research into novel methods to reduce host exposure. food-grade microbes that can be delivered to the gastrointestinal tract and that are capable of sequestering toxins present a safe and cost-effective intervention. we sought to investigate the potential for probiotic-supplemented yo ...201425293764
cholesterol assimilation by lactobacillus probiotic bacteria: an in vitro investigation.excess cholesterol is associated with cardiovascular diseases (cvd), an important cause of mortality worldwide. current cvd therapeutic measures, lifestyle and dietary interventions, and pharmaceutical agents for regulating cholesterol levels are inadequate. probiotic bacteria have demonstrated potential to lower cholesterol levels by different mechanisms, including bile salt hydrolase activity, production of compounds that inhibit enzymes such as 3-hydroxy-3-methylglutaryl coenzyme a, and chole ...201425295259
probiotic lactobacillus rhamnosus gg (lgg) and prebiotic prevent neonatal inflammation-induced visceral hypersensitivity in adult rats.increasing evidence indicates a positive effect of probiotics on the nervous system. the objective of this study was to determine if probiotic lactobacillus rhamnosus gg (lgg) and/or prebiotics polydextrose/galactooligosaccharide (pdx/gos) can alter the colonic sensitivity in a neonatal rat model of chronic visceral hyperalgesia and to determine whether altered sensitivity is associated with changes in neurotransmitter levels in the brain.201425298006
the effect of lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model.growing evidence indicates that intestinal microbiota regulate our metabolism. probiotics confer health benefits that may depend on their ability to affect the gut microbiota. the objective of this study was to examine the effect of supplementation with the probiotic strain, lactobacillus rhamnosus hsryfm 1301, on the gut microbiota in a hyperlipidemic rat model, and to explore the associations between the gut microbiota and the serum lipids.201425300818
oral intake of lactobacillus rhamnosus m21 enhances the survival rate of mice lethally infected with influenza virus.influenza viruses cause acute respiratory disease. because of the high genetic variability of viruses, effective vaccines and antiviral agents are limited. considering the fact that the site of influenza virus entry is the mucosa of the upper respiratory tract, probiotics that can enhance mucosal immunity as well as systemic immunity could be an important source of treatment against influenza infection.201625304268
biosorption of al(+3) and cd(+2) by an exopolysaccharide from lactobacillus rhamnosus.the aim of this study was to assess the removal of cd(2+) and al(3+) from aqueous solutions by an exopolysaccharide (eps) from lactobacillus rhamnosus e/n. the biosorption kinetics of eps for cd(2+) and al(3+) were studied by equilibrium dialysis after incubation from 0 h to 58 h. the actual concentrations of cd(2+) and al(3+) in external solutions were measured by a fluorescence method after complexation with 8-hydroxyquinoline. binding of metal ions by eps was observed, with maximum adsorption ...201425308465
prebiotic and synbiotic effects on rats fed malted barley with selected bacteria strains.butyric acid, one of the key products formed when β-glucans are degraded by the microbiota in the colon, has been proposed to be important for colonic health. glutamine bound to the fibre may have similar effects once it has been liberated from the fibre in the colon. both β-glucans and glutamine are found in high amounts in malted barley. lactobacillus rhamnosus together with malt has been shown to increase the formation of butyric acid further in rats.201425317120
probiotics for preventing ventilator-associated pneumonia.ventilator-associated pneumonia (vap) is common in intensive care units (icus). some evidence indicates that probiotics may reduce the incidence of vap. several additional published studies have demonstrated that probiotics are safe and efficacious in preventing vap in icus. we aimed to systematically summarise the results of all available data to generate the best evidence for the prevention of vap.201425344083
probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs.we generated a neonatal pig model with human infant gut microbiota (hgm) to study the effect of a probiotic on the composition of the transplanted microbiota following rotavirus vaccination and challenge. all the hgm-transplanted pigs received two doses of an oral attenuated rotavirus vaccine. the gut microbiota of vaccinated pigs were investigated for effects of lactobacillus rhamnosus gg (lgg) supplement and homotypic virulent human rotavirus (hrv) challenge. high-throughput sequencing of v4 r ...201425349634
adhesion properties of lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins.we previously described potential probiotic lactobacillus rhamnosus strains, isolated from fermented mare milk produced in sumbawa island, indonesia, which showed high adhesion to porcine colonic mucin (pcm) and extracellular matrix (ecm) proteins. recently, mucus-binding factor (mbf) was found in the gg strain of l. rhamnosus as a mucin-binding protein. in this study, we assessed the ability of recombinant mbf protein from the fsmm22 strain, one of the isolates of l. rhamnosus from fermented su ...201525351253
survival of pathogenic and lactobacilli species of fermented olives during simulated human digestion.the present survey uses a dynamic gastric and small intestinal model to assess the survival of one pathogenic (escherichia coli o157:h7 edl 933) and three lactobacilli bacteria with probiotic potential (lactobacillus rhamnosus gg, l. pentosus tomc-lab2, and l. pentosus tomc-lab4) during their passage through the human gastrointestinal tract using fermented olives as the food matrix. the data showed that the survival of the e. coli strain in the stomach and duodenum was very low, while its transi ...201425352842
the effect of a multi-strain probiotic on the resistance toward escherichia coli challenge in a randomized, placebo-controlled, double-blind intervention study.several probiotic strains have been shown to enhance human resistance to infectious disease. it is speculated that these strains may impose this effect by excretion of anti-microbial components, by competing with pathogens for intestinal nutrients and/or mucosal adhesion sites or modulating the immune system.201525369827
reduced neonatal regulatory t cell response to microbial stimuli associates with subsequent eczema in high-risk infants.regulatory t cells (treg) play an essential role in early immune programming and shaping the immune response towards a pro-allergic or tolerant state. we evaluated cord blood treg and cytokine responses to microbial and non-microbial stimuli in infants at high risk of allergic disease and their associations with development of allergic disease in the first year.201425376403
effects of korean red ginseng (panax ginseng), urushiol (rhus vernicifera stokes), and probiotics (lactobacillus rhamnosus r0011 and lactobacillus acidophilus r0052) on the gut-liver axis of alcoholic liver disease.roles of immune reaction and toll-like receptor-4 (tlr-4) have widely been established in the pathogenesis of alcoholic liver disease (ald).201425378990
impact of different cryoprotectants on the survival of freeze-dried lactobacillus rhamnosus and lactobacillus casei/paracasei during long-term storage.the production of long shelf-life highly concentrated dried probiotic/starter cultures is of paramount importance for the food industry. the aim of the present study was to evaluate the protective effect of glucose, lactose, trehalose, and skim milk applied alone or combined upon the survival of potentially probiotic lactobacillus rhamnosus ctc1679, lactobacillus casei/paracasei ctc1677 and l. casei/paracasei ctc1678 during freeze-drying and after 39 weeks of storage at 4 and 22 °c. immediately ...201525380798
inhibitory activity in vitro of probiotic lactobacilli against oral candida under different fermentation conditions.clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral candida species. sugar utilisation by both lactobacilli and candida was also assessed. agar overlay assay was utilised to study growth inhibition of candida albicans, candida glabrata and candida krusei by lactobacillus rhamnosus gg, ...201525380800
gut commensal microvesicles reproduce parent bacterial signals to host immune and enteric nervous systems.ingestion of a commensal bacteria, lactobacillus rhamnosus jb-1, has potent immunoregulatory effects, and changes nerve-dependent colon migrating motor complexes (mmcs), enteric nerve function, and behavior. how these alterations occur is unknown. jb-1 microvesicles (mvs) are enriched for heat shock protein components such as chaperonin 60 heat-shock protein isolated from escherichia coli (groel) and reproduce regulatory and neuronal effects in vitro and in vivo. ingested labeled mvs were detect ...201525392266
phenotypical analysis of the lactobacillus rhamnosus gg fimbrial spafed operon: surface expression and functional characterization of recombinant spafed pili in lactococcus lactis.a noticeable genomic feature of many piliated gram-positive bacterial species is the presence of more than one pilus-encoding operon. paradigmatically, the gut-adapted lactobacillus rhamnosus gg strain contains two different fimbrial operons in its genome. however, whereas one of these operons (called spacba) is encoding for the functionally mucus-/collagen-binding spacba pilus, for the other operon (called spafed) any native expression of the spafed-called pili is still the subject of some unce ...201425415357
effect of lactobacillus rhamnosus hsryfm 1301 on the gut microbiota and lipid metabolism in rats fed a high-fat diet.accumulating evidence indicates that lactic acid bacteria could improve host physiology and lipid metabolism. to investigate the effect of the gut microbiota on host lipid metabolism, a hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 days, and the gut microbiota of the rats was analyzed using real-time pcr before and after administration of lactobacillus rhamnosus hsryfm 1301 and its fermented milk for 28 days. the findings showed that the lactobacillus spp., bifi ...201525418480
efficacy of corn silage inoculants on the fermentation quality under farm conditions and their influence on aspergillus parasitucus, a. flavus and a. fumigatus determined by q-pcr.laboratory-scale silos were prepared to evaluate the efficacy of two different lactic acid bacteria (lab) on the fermentation quality and mycobiota of corn silage. their influence on aspergillus species' variability by using the q-pcr technique was studied. silage inoculated with lactobacillus rhamnosus rc007 or l. plantarum rc009 were compared with uninoculated silage. silos were opened after 1, 7, 45, 90 and 120 days after ensiling. at the end of the ensiling period, silos were left open for 7 ...201525421370
molecular characterization of lactobacillus plantarum dmdl 9010, a strain with efficient nitrite degradation capacity.nitrites commonly found in food, especially in fermented vegetables, are potential carcinogens. therefore, limiting nitrites in food is critically important for food safety. a lactobacillus strain (lactobacillus sp. dmdl 9010) was previously isolated from fermented vegetables by our group, and is not yet fully characterized. a number of phenotypical and genotypical approaches were employed to characterize lactobacillus sp. dmdl 9010. its nitrite degradation capacity was compared with four other ...201425423449
bovine milk fermented with lactobacillus rhamnosus l8020 decreases the oral carriage of mutans streptococci and the burden of periodontal pathogens.the aim of this study was to find the oral isolate of lactobacilli, which has the potential to inhibit either periodontal, cariogenic, or fungal pathogens in vitro, and to examine the effects of bovine milk fermented with the isolate on the oral carriage of cariogenic and periodontal pathogens.201125426790
brain and liver fatty acid composition changes upon consumption of lactobacillus rhamnosus la68.recent reports suggest that the metabolic activity of the enteric microbiota may influence the fatty acid composition of the host tissue. there are many studies dealing with the influence of lactobacilli on various pathological conditions, and some of the effects are strain-specific. this study was designed to test the effects of a particular lactobacillus strain, lactobacillus rhamnosus la68 on fatty acid composition of the liver and the brain of c57bl/6 mice in the absence of an underlying pat ...201525431181
lactobacillus rhamnosus gg reverses insulin resistance but does not block its onset in diet-induced obese mice.recently, lactobacillus rhamnosus gg (lgg) was shown to exert insulin-sensitizing and adiposity-reducing effects in high-fat (hf) diet-fed mice. in the present study, we observed that the effects were correlated with the extent of dysbiosis induced by hf diet feeding before lgg administration. lgg-treated mice were protected from hf diet-induced adiposity and/ or insulin resistance when lgg was treated after, not along with, hf diet feeding. results indicate that, under hf dietary condition, sup ...201525433553
differential immune-stimulatory effects of ltas from different lactic acid bacteria via mapk signaling pathway in raw 264.7 cells.lipoteichoic acid (lta) is an immune-stimulatory component found in the cell wall of lactic acid bacteria, which are a major group of gram-positive bacteria known to have beneficial health effects in humans. in this study, we evaluated the stimulatory effects of ltas isolated from different lactobacilli species with mouse macrophage raw 264.7 cells.201525433634
no evidence of harms of probiotic lactobacillus rhamnosus gg atcc 53103 in healthy elderly-a phase i open label study to assess safety, tolerability and cytokine responses.although lactobacillus rhamnosus gg atcc 53103 (lgg) has been consumed by 2 to 5 million people daily since the mid 1990s, there are few clinical trials describing potential harms of lgg, particularly in the elderly.201425438151
decrease in lactobacilli in the intestinal microbiota of celiac children with a gluten-free diet, and selection of potentially probiotic strains.the intestinal microbiota would be implicated in pathology associated with celiac disease caused by an abnormal immune system reaction against gluten present in cereal grains. the objectives of this work were to detect through basic methods the changes in the composition of the most common genera of bacteria from the intestinal microbiota of symptom-free celiac disease children with a gluten-free diet compared with healthy children from tucumán and to select lactobacilli (lb) strains with probio ...201525438612
effects of immunomodulatory supplementation with lactobacillus rhamnosus on airway inflammation in a mouse asthma model.asthma is a common allergic disease. in previous studies, probiotics improved the balance of intestinal microbes, reduced inflammation, and promoted mucosal tolerance. this study investigated whether oral administrations of lactobacillus rhamnosus gg (lgg) inhibited allergen (ovalbumin or ova)-induced airway inflammation in a mouse asthma model.201625440975
molecular characteristics of an exopolysaccharide from lactobacillus rhamnosus kf5 in solution.the molecular weight and chain conformation of an exopolysaccharide (eps), s2, from lactobacillus rhamnosus kf5 were determined by size-exclusion chromatography combined with multi-angle laser light scattering (sec-malls), dynamic light scattering (dls), viscometry and atomic force microscopy (afm). the weight-average molecular weight (mw), intrinsic viscosity [ƞ], radii of gyration (r(g)) and hydrodynamic radii (r(h)) were 7.5 × 10(5) da, 2.331 dl/g, 44.3 nm and 29.4 nm, respectively. the confo ...201525453290
probiotics: an update.triggered by the growing knowledge on the link between the intestinal microbiome and human health, the interest in probiotics is ever increasing. the authors aimed to review the recent literature on probiotics, from definitions to clinical benefits, with emphasis on children.201525458874
ehealth: low fodmap diet vs lactobacillus rhamnosus gg in irritable bowel syndrome.to investigate the effects of a low fermentable, oligosaccharides, disaccharides, monosaccharides and polyols diet (lfd) and the probiotic lactobacillus rhamnosus gg (lgg) in irritable bowel syndrome (ibs).201425473176
the influence of the wooden equipment employed for cheese manufacture on the characteristics of a traditional stretched cheese during ripening.the influence of the wooden equipment used for the traditional cheese manufacturing from raw milk was evaluated on the variations of chemico-physical characteristics and microbial populations during the ripening of caciocavallo palermitano cheese. milk from two farms (a, extensive; b, intensive) was processed in traditional and standard conditions. chemical and physical traits of cheeses were affected by the farming system and the cheese making technology, and changed during ripening. content in ...201525475270
the use of flow cytometry to accurately ascertain total and viable counts of lactobacillus rhamnosus in chocolate.the goals of this study were to evaluate the precision and accuracy of flow cytometry (fc) methodologies in the evaluation of populations of probiotic bacteria (lactobacillus rhamnosus r0011) in two commercial dried forms, and ascertain the challenges in enumerating them in a chocolate matrix. fc analyses of total (fc(t)) and viable (fc(v)) counts in liquid or dried cultures were almost two times more precise (reproducible) than traditional direct microscopic counts (dcm) or colony forming units ...201525475282
high resolution melting analysis (hrm) as a new tool for the identification of species belonging to the lactobacillus casei group and comparison with species-specific pcrs and multiplex pcr.the correct identification and characterisation of bacteria is essential for several reasons: the classification of lactic acid bacteria (lab) has changed significantly over the years, and it is important to distinguish and define them correctly, according to the current nomenclature, avoiding problems in the interpretation of literature, as well as mislabelling when probiotic are used in food products. in this study, species-specific pcr and hrm (high-resolution melting) analysis were developed ...201525475306
characterization of the antifungal activity of lactobacillus harbinensis k.v9.3.1np and lactobacillus rhamnosus k.c8.3.1i in yogurt.few antifungal protective cultures adapted to fermented dairy products are commercially available because of the numerous constraints linked to their market implementation. consumer's demand for naturally preserved food products is growing and the utilization of lactic acid bacteria is a promising way to achieve this goal. in this study, using a 2(5-1) factorial fractional design, we first evaluated the effects of fermentation time, of initial sucrose concentration and of the initial contaminati ...201525481057
lactobacilli and bifidobacteria enhance mucosal b cell responses and differentially modulate systemic antibody responses to an oral human rotavirus vaccine in a neonatal gnotobiotic pig disease model.b cells play a key role in generation of protective immunity against rotavirus infection, a major cause of gastroenteritis in children. current rv vaccines are less effective in developing countries compared to developed countries. commensals/probiotics influence mucosal immunity, but the role of early gut colonizing bacteria in modulating intestinal b cell responses to rv vaccines is largely unknown. we co-colonized neonatal gnotobiotic pigs, the only animal model susceptible to hrv diarrhea, w ...201425483333
potentially probiotic and bioprotective lactic acid bacteria starter cultures antagonise the listeria monocytogenes adhesion to ht29 colonocyte-like cells.the capability of five lactic acid bacteria (lab) to counteract the adhesion of listeria monocytogenes to the epithelial intestinal cell line ht29 was studied. the highest adhesion ability to ht29 was achieved by the intestinal strain lactobacillus rhamnosus ctc1679, followed by the meat-derived strains lactobacillus sakei ctc494 and enterococcus faecium ctc8005. surprisingly, the meat strains showed significantly better adhesion to ht29 than two faecal isolates of lactobacillus casei and even s ...201525488261
physicochemical characterization of exopolysaccharides produced by lactobacillus rhamnosus on various carbon sources.the impact of five carbohydrate sources (glucose, maltose, galactose, sucrose, and lactose) on the chemical composition, structure, morphology, and physicochemical properties, as well as, viscosity of exopolysaccharides (epss) produced by lactobacillus rhamnosus e/n was investigated. glc-ms analysis and 2dnmr spectroscopy showed that the epss had the same primary structure independently of the carbon source used in the growth medium. the following eps composition was elucidated: four rhamnose, t ...201525498664
lactobacillus rhamnosus cncm i-3690 and the commensal bacterium faecalibacterium prausnitzii a2-165 exhibit similar protective effects to induced barrier hyper-permeability in mice.impaired gut barrier function has been reported in a wide range of diseases and syndromes and in some functional gastrointestinal disorders. in addition, there is increasing evidence that suggests the gut microbiota tightly regulates gut barrier function and recent studies demonstrate that probiotic bacteria can enhance barrier integrity. here, we aimed to investigate the effects of lactobacillus rhamnosus cncm i-3690 on intestinal barrier function. in vitro results using a caco-2 monolayer cell ...201525517879
lactobacillus rhamnosus la68 and lactobacillus plantarum wcfs1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis.in this study, two lactobacillus strains (l. rhamnosus la68 and l. plantarum wcfs1) were evaluated for their effects on high fat diet induced pathology in mice. the aim was to determine whether the administration of lactic acid bacteria had beneficial effects on ameliorating pathology. c57bl/6 mice fed a high fat diet were orally administered with the lactobacillus strains. both the metabolic and immunological parameters were analyzed. the administration of both of the strains had beneficial eff ...201525518825
growth and safety evaluation of infant formulae containing oligosaccharides derived from bovine milk: a randomized, double-blind, noninferiority trial.a limited number of nondigestible oligosaccharides are available for use in infant formula. this study evaluated growth and safety in infants fed formula supplemented with a mixture of bovine milk-derived oligosaccharides (bmos). this mixture, which was generated from whey permeate, contains galactooligosaccharides and other oligosaccharides from bovine milk, such as 3'- and 6'-sialyllactose. we hypothesized that growth in infants fed bmos-supplemented formula would be noninferior to that in inf ...201425527244
uncovering surface-exposed antigens of lactobacillus rhamnosus by cell shaving proteomics and two-dimensional immunoblotting.the present study reports the identification and comparison of all expressed cell-surface exposed proteins from the well-known probiotic l. rhamnosus gg and a related dairy strain, lc705. to obtain this information, the cell-surface bound proteins were released from intact cells by trypsin shaving under hypertonic conditions with and without dtt. liquid chromatography tandem mass spectrometry (lc-ms/ms) analyses of the purified peptides identified a total of 102 and 198 individual proteins from ...201525531588
use of lactobacillus casei rhamnosus to prevent cholangitis in biliary atresia after kasai operation.recurrent cholangitis may aggravate cholestatic liver cirrhosis in biliary atresia (ba) after the kasai operation. this pilot study aimed to investigate whether lactobacillus casei rhamnosus has the prophylactic efficacy for recurrent cholangitis in comparison with the conventional neomycin prophylaxis.201525534776
cholesterol-lowering potentials of lactic acid bacteria based on bile-salt hydrolase activity and effect of potent strains on cholesterol metabolism in vitro and in vivo.this study collected different probiotic isolates from animal and plant sources to evaluate the bile-salt hydrolase activity of probiotics in vitro. the deconjugation potential of bile acid was determined using high-performance liquid chromatography. hepg2 cells were cultured with probiotic strains with high bsh activity. the triglyceride (tg) and apolipoprotein b (apo b) secretion by hepg2 cells were evaluated. our results show that the bsh activity and bile-acid deconjugation abilities of pedi ...201425538960
antimicrobial activity of human β-defensins against lactic acid bacteria.in this study, we evaluated the antimicrobial activity of human β-defensin-1 (hbd-1), human β-defensin-2 (hbd-2) and human β-defensin-3 (hbd-3) against three internationally common probiotic strains of lactic acid bacterium. our results indicated that hbd-1, hbd-2 and hbd-3 at the range of 0.08-10 μg/ml do not have obvious antimicrobial activity against these strains. viability of bifidobacterium longum jdm301 (b. longum jdm301), bifidobacterium lactis hn019 (b. lactis hn019) and lactobacillus r ...201525560646
production of lactic acid from the mixture of softwood pre-hydrolysate and paper mill sludge by simultaneous saccharification and fermentation.paper mill sludge is a solid waste material composed of pulp residues and ash generated from pulping and paper making process. the carbohydrate portion of the sludges from kraft/recycle paper mill has chemical and physical characteristics similar to those of commercial wood pulp. because of its high carbohydrate content and well-dispersed structure, the sludge can be biologically converted to value-added products without pretreatment. in bioconversion of solid feedstock such as paper mill sludge ...201525561054
microencapsulation of alginate-immobilized bagasse with lactobacillus rhamnosus nrrl 442: enhancement of survivability and thermotolerance.the aim of this research was to enhance the survivability of lactobacillus rhamnosus nrrl 442 against heat exposure via a combination of immobilization and microencapsulation processes using sugarcane bagasse (sb) and sodium alginate (naa), respectively. the microcapsules were synthesized using different alginate concentration of 1, 2 and 3% and naa:sb ratio of 1:0, 1:1 and 1:1.5. this beneficial step of probiotic immobilization before microencapsulation significantly enhanced microencapsulation ...201525563958
lactobacillus rhamnosus gg dosage affects the adjuvanticity and protection against rotavirus diarrhea in gnotobiotic pigs.the use of immunostimulatory strains of probiotics as adjuvants has been increasingly recognized as a promising approach in enhancing vaccine immunogenicity; however, dose effects of probiotic adjuvants are not well defined. in the present study, we examined dose effects of a commonly used probiotic strain, lactobacillus rhamnosus gg (lgg), on immunomodulation with 2 different dosages.201525564808
piliation of lactobacillus rhamnosus gg promotes adhesion, phagocytosis, and cytokine modulation in macrophages.recently, spacba-encoded pili on the cell surface of lactobacillus rhamnosus gg were identified to be key molecules for binding to human intestinal mucus and caco-2 intestinal epithelial cells. here, we investigated the role of the spacba pilus of l. rhamnosus gg in the interaction with macrophages in vitro by comparing the wild type with surface mutants. our results show that spacba pili play a significant role in the capacity for adhesion to macrophages and also promote bacterial uptake by the ...201525576613
lactobacillus rhamnosus gg and its spac pilus adhesin modulate inflammatory responsiveness and tlr-related gene expression in the fetal human gut.bacterial contact in utero modulates fetal and neonatal immune responses. maternal probiotic supplementation reduces the risk of immune-mediated disease in the infant. we investigated the immunomodulatory properties of live lactobacillus rhamnosus gg and its spac pilus adhesin in human fetal intestinal models.201525580735
can probiotic yogurt prevent diarrhoea in children on antibiotics? a double-blind, randomised, placebo-controlled study.to estimate the efficacy of a probiotic yogurt compared to a pasteurised yogurt for the prevention of antibiotic-associated diarrhoea in children.201525588782
in vitro characterization of the digestive stress response and immunomodulatory properties of microorganisms isolated from smear-ripened cheese.thirty-six microorganisms (twenty-one bacteria, twelve yeasts and three fungi) were isolated from surface-ripened cheeses and subjected to in vitro digestive stress. the approach mimicked gastric and/or duodenal digestion. lactobacillus rhamnosus gg, escherichia coli nissle 1917 and saccharomyces boulardii were used as reference strains. we studied the microorganisms grown separately in culture medium and then included (or not) in a rennet gel. the microorganisms' immunomodulatory abilities were ...201525589362
hepatic and fecal metabolomic analysis of the effects of lactobacillus rhamnosus gg on alcoholic fatty liver disease in mice.the interactions among the gut, liver, and immune system play an important role in liver disease. probiotics have been used for the treatment and prevention of many pathological conditions, including liver diseases. comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (gc×gc-tof ms) was used herein, in conjunction with chemometric data analysis, to identify metabolites significantly affected by probiotics in mice fed with or without alcohol. the metabolomics analysis ...201525592873
administration of a probiotic with peanut oral immunotherapy: a randomized trial.coadministration of a bacterial adjuvant with oral immunotherapy (oit) has been suggested as a potential treatment for food allergy.201525592987
purification, crystallization and preliminary x-ray diffraction analysis of spad, a backbone-pilin subunit encoded by the fimbrial spafed operon in lactobacillus rhamnosus gg.spad is the predicted backbone-pilin subunit of the spafed pilus, whose loci are encoded by the fimbrial spafed operon in lactobacillus rhamnosus gg, a gram-positive gut-adapted commensal strain with perceived probiotic benefits. in this study, soluble recombinant spad protein was overproduced in escherichia coli and then purified by ni2+-chelating affinity and gel-filtration chromatography. after limited proteolysis with α-chymotrypsin, good-quality crystals of spad were obtained which diffract ...201525615979
enhanced ampk phosphorylation contributes to the beneficial effects of lactobacillus rhamnosus gg supernatant on chronic-alcohol-induced fatty liver disease.we have previously demonstrated that lactobacillus rhamnosus gg culture supernatant (lggs) prevents acute-alcohol-exposure-induced hepatic steatosis and injury. the protective effects of lggs were attributed to the improved intestinal barrier function leading to decreased endotoxemia. the purpose of this study was to determine whether lggs was effective in protecting against chronic-alcohol-induced hepatic steatosis and injury and to evaluate the underlying mechanisms of lggs on hepatic lipid me ...201525622859
administration of probiotics lactobacillus rhamnosus gg and lactobacillus gasseri k7 during pregnancy and lactation changes mouse mesenteric lymph nodes and mammary gland microbiota.the milk and mammary gland (mg) microbiome can be influenced by several factors, such as mode of delivery, breastfeeding, maternal lifestyle, health status, and diet. an increasing number of studies show a variety of positive effects of consumption of probiotics during pregnancy and breastfeeding on the mother and the newborn. the aim of this study was to investigate the effect of oral administration of probiotics lactobacillus gasseri k7 (lk7) and lactobacillus rhamnosus gg (lgg) during pregnan ...201525622869
modulatory activity of lactobacillus rhamnosus oll2838 in a mouse model of intestinal immunopathology.gut microbiota and probiotic strains play an important role in oral tolerance by modulating regulatory and effector cell components of the immune system. we have previously described the ability of lactobacilli to influence both the innate and adaptive immunity to wheat gluten, a food antigen, in mouse. in this study, we further explored the immunomodulatory mechanisms elicited in this model by testing three specific probiotic strains, namely l. rhamnosus oll2838, b. infantis atcc15697 and s. th ...201525623030
functional properties of peanut fractions on the growth of probiotics and foodborne bacterial pathogens.various compounds found in peanut (arachis hypogaea) have been shown to provide multiple benefits to human health and may influence the growth of a broad range of gut bacteria. in this study, we investigated the effects of peanut white kernel and peanut skin on 3 strains of lactobacillus and 3 major foodborne enteric bacterial pathogens. significant (p < 0.05) growth stimulation of lactobacillus casei and lactobacillus rhamnosus was observed in the presence of 0.5% peanut flour (pf) made from pe ...201525627431
influence of orally administered lactobacillus gg on respiratory immune response in a murine model of diet-induced obesity.mice with diet-induced obesity were fed with lactobacillus rhamnosus gg (lgg) suspended in saline or saline alone (control mice). pulmonary mrna expression of ifn-γ; ifn-α receptor 1; cd247 antigen; killer cell lectin-like receptor subfamily k, member 1; tnf-α; il-12 receptor β1 and il-2 receptor β, and the proportion of lactobacillales in feces were significantly greater in the lgg group than in the control mice (p < 0.05 and p < 0.01, respectively). these results suggest that lgg alters the re ...201525643737
fermented red ginseng extract inhibits cancer cell proliferation and viability.red ginseng (panax ginseng c.a. meyer) is the most widely recognized medicinal herb due to its remedial effects in various disorders, such as cancers, diabetes, and heart problems. in this study, we investigated the anticancer effect of fermented red ginseng extract (f-rge; provided by jeonju biomaterials institute, jeonju, south korea) in a parallel comparison with the effect of nonfermented red ginseng extract (nf-rge; control) on several cancer cell lines--mcf-7 breast cancer cells, hepg2 hep ...201525658580
[probiotics for the treating acute diarrhea and preventing antibiotic-associated diarrhea in children].probiotics are helpful in the treatment of acute diarrhea. several systematic reviews show that the use of probiotics shortens the duration of diarrhea in one day and reduces by 59% the risk of diarrhea lasting longer than 4 days. hospital stay is 24 hours shorter in children treated with probiotics. the benefitial effect of probiotics is species-specific; lactobacillus rhamnosus gg and saccharomyces boulardii are the two species with proven efficacy in the treatment of acute diarrhea. the use o ...201525659056
[irritable bowel syndrome; gut microbiota and probiotic therapy].irritable bowel syndrome (ibs) is characterized by symptoms of abdominal pain and altered bowel habits. this common disorder is managed by varying clinical styles as no dominant therapeutic strategy has emerged. the pathophysiology of ibs remains unknown, but several lines of evidence link this disorder with the gut microbiota. although controversy exists, gut microbiota is likely contributing to symptoms of ibs, at least in some patients, through an altered fermentation process, an impaired int ...201525659060
nonpharmacologic treatment of functional abdominal pain disorders: a systematic review.various nonpharmacologic treatments are available for pediatric abdominal pain-related functional gastrointestinal disorders (ap-fgids). data on efficacy and safety are scarce. the goal of this study was to summarize the evidence regarding nonpharmacologic interventions for pediatric ap-fgids: lifestyle interventions, dietary interventions, behavioral interventions, prebiotics and probiotics, and alternative medicine.201525667239
emergency department treatment of children with diarrhea who attend day care: a randomized multidose trial of a lactobacillus helveticus and lactobacillus rhamnosus combination probiotic.benefits associated with probiotic administration to children seeking emergency department care with diarrheal disease are unknown.201525669920
probiotic lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing candida glabrata isolates.this study investigates the antagonistic effects of the probiotic strains lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 against vulvovaginal candidiasis (vvc)-causing candida glabrata.201525688886
probiotics (lactobacillus rhamnosus r0011 and acidophilus r0052) reduce the expression of toll-like receptor 4 in mice with alcoholic liver disease.the role of lipopolysaccharide (lps) and toll-like receptor 4 (tlr 4) in the pathogenesis of alcoholic liver disease (ald) has been widely established. we evaluated the biological effects of probiotics (lactobacillus rhamnosus r0011 and acidophilus r0052), krg (korea red ginseng), and urushiol (rhus verniciflua stokes) on ald, including their effects on normal and high-fat diet in mice.201525692549
improved in vitro assay for determining the mucin adherence of bacteria sensitive to triton x-100 treatment.mucin-associated microbiota are in relatively close contact with the intestinal epithelium and may thus have a more pronounced effect on host health. we have previously developed a simple mucin agar assay to simulate initial mucus colonization by intestinal microbial communities. adherence of microbiota was estimated using flow cytometry after detachment with triton x-100. in this study, the effect of this detergent on the cultivability of both virulent and commensal strains was investigated. mu ...201525702162
probiotics reduce necrotizing enterocolitis severity in hiv-exposed premature infants.to assess the effect of probiotics on the incidence of necrotizing enterocolitis (nec) in premature infants born to human immunodeficiency virus (hiv)-positive and hiv-negative women.201525724213
effects of lactobacillus plantarum a7 with probiotic potential on colon cancer and normal cells proliferation in comparison with a commercial strain.several beneficial effects have been attributed to the probiotic lactic acid bacteria. it was determined that lactobacilli can exert antiproliferative effects on the various cancer cell lines including colon cancer. effects of lactic acid bacteria on colon cancer may vary from strain to strain and there is a need to find the new probiotic strains with tumor suppressing properties through in vitro studies.201425729553
non-viable immunobiotic lactobacillus rhamnosus crl1505 and its peptidoglycan improve systemic and respiratory innate immune response during recovery of immunocompromised-malnourished mice.the effect of non-viable lactobacillus rhamnosus crl1505 and its cell wall and peptidoglycan on respiratory immunity in malnourished mice was studied. weaned mice were malnourished with a protein-free diet for 21d and received bcd during 7d (bcd) or bcd with nasal non-viable l. rhamnosus crl1505 (bcd+uv) or its cell wall (bcd+cw) or peptidoglycan (bcd+pg) supplementation during last 2d of the treatment. malnourished mice without treatment (mnc) and well-nourished mice (wnc) were used as controls ...201525744605
evaluation of the probiotic properties of new lactobacillus and bifidobacterium strains and their in vitro effect.probiotic ingestion is recommended as a preventive approach to maintain the balance of the intestinal microbiota and to enhance the human well-being. during the whole life of each individual, the gut microbiota composition could be altered by lifestyle, diet, antibiotic therapies and other stress conditions, which may lead to acute and chronic disorders. hence, probiotics can be administered for the prevention or treatment of some disorders, including lactose malabsorption, acute diarrhoea, irri ...201525744647
inhibition of mir122a by lactobacillus rhamnosus gg culture supernatant increases intestinal occludin expression and protects mice from alcoholic liver disease.alcoholic liver disease (ald) has a high morbidity and mortality. chronic alcohol consumption causes disruption of intestinal microflora homeostasis, intestinal tight junction barrier dysfunction, increased endotoxemia, and eventually liver steatosis/steatohepatitis. probiotic lactobacillus rhamnosus gg (lgg) and the bacteria-free lgg culture supernatant (lggs) have been shown to promote intestinal epithelial integrity and protect intestinal barrier function in ald. however, little is known abou ...201525746479
a possible link between early probiotic intervention and the risk of neuropsychiatric disorders later in childhood: a randomized trial.recent experimental evidence suggests that gut microbiota may alter function within the nervous system providing new insight on the mechanism of neuropsychiatric disorders.201525760553
cost-effectiveness of using an extensively hydrolyzed casein formula plus the probiotic lactobacillus rhamnosus gg compared to an extensively hydrolyzed formula alone or an amino acid formula as first-line dietary management for cow's milk allergy in the us.the aim was to estimate the cost-effectiveness of using an extensively hydrolyzed casein formula (ehcf) plus the probiotic lactobacillus rhamnosus gg (ehcf + lgg; nutramigen lgg) compared to an ehcf alone (nutramigen) and an amino acid formula (aaf; neocate) as first-line dietary management for cow's milk allergy (cma) in the us.201525767400
characterization of lactobacillus isolated from dairy samples for probiotic properties.in the present study twelve lactobacillus isolates (lbs 1-lbs 12) were characterized for probiotic properties. out of the twelve, eight isolates (lbs 1-6, 8 and 11) were bile resistant (survival > 50% at 0.3% bile salt w/v) and five isolates (lbs 1, 2, 5, 6 and 11) were found acid ph value resistant (survival > 50% at ph 3). all twelve isolates inhibited the growth of staphylococcus aureus whereas isolate lbs 2 also inhibited the growth of escherichia coli and salmonella typhimurium. antibiotic ...201525771244
differential effects of two probiotics on the risks of eczema and atopy associated with single nucleotide polymorphisms to toll-like receptors.there is strong evidence to support a genetic predisposition to eczema and more recently studies have suggested that probiotics might be used to prevent eczema by modifying the expression of putative allergy-associated genes. the aim of this present study was to investigate whether two probiotics, lactobacillus rhamnosus hn001 (hn001) and bifidobacterium animalis subsp. lactis hn019 (hn019), can modify the known genetic predisposition to eczema conferred by genetic variation in the toll-like rec ...201525779902
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