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isolation, characterization and phylogeny of sponge-associated bacteria with antimicrobial activities from brazil.bacteria associated with marine sponges represent a rich source of bioactive metabolites. the aim of this study was to isolate and characterize bacteria with antimicrobial activities from brazilian sponges. a total of 158 colony-forming units were isolated from nine sponge species. among these, 12 isolates presented antimicrobial activities against pathogenic bacteria. based on comparative sequence analysis of their 16s rrna genes, the sponge-associated bacterial strains could be subdivided into ...201020600863
[analysis of acquired resistance genes in stenotrophomonas maltophilia].stenotrophomonas maltophilia is a gram-negative bacillus and a nosocomial pathogen in immunocompromised patients. trimethoprim/sulfamethoxazole (tmp/smx) is the drug of choice for treating s. maltophilia infection; however, resistance to tmp/smx is increasing. in this study, we investigated the relationship between the incidence of tmp/smx resistance and the presence of sul genes and mobile elements.201020603591
newer antibacterial agents and their potential role in cystic fibrosis pulmonary exacerbation management.pulmonary exacerbations in cystic fibrosis (cf) are frequent events and account for a substantial proportion of the burden of morbidity and mortality in this disease. antibacterial therapies to treat pulmonary exacerbations are instituted empirically and are individualized based on both patient factors (severity of exacerbation, frequency of exacerbation, recent courses of anti-infectives) and pathogen factors (previously isolated pathogens and in vitro predicted susceptibilities). however, the ...201020605846
diffusion measurements inside biofilms by image-based fluorescence recovery after photobleaching (frap) analysis with a commercial confocal laser scanning microscope.research about the reactional and structural dynamics of biofilms at the molecular level has made great strides, owing to efficient fluorescence imaging methods in terms of spatial resolution and fast acquisition time but also to noninvasive conditions of observation consistent with in situ biofilm studies. in addition to conventional fluorescence intensity imaging, the fluorescence recovery after photobleaching (frap) module can now be routinely implemented on commercial confocal laser scanning ...201020639359
[investigation of distribution of bacteria and fungi in severe acute pancreatitis].to investigate the spectrum of bacteria and fungi in different sites in severe acute pancreatitis (sap).201020646657
post-antibiotic effect (pae) of moxifloxacin in multidrug-resistant stenotrophomonas maltophilia. 201020674284
rapid detection of pseudomonas aeruginosa from positive blood cultures by quantitative pcr.pseudomonas aeruginosa is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. the present study was aimed to develop a novel quantitative pcr (qpcr) assay, using ecfx as the specific target gene, for the rapid and accurate identification of p. aeruginosa from positive blood cultures (bcs).201020684778
[biofilm on a metal surface as a factor of microbial corrosion].main attention was given in the present review to the research methods, phases of biofilm's forming, exopolymer compounds of bacteria as main biofilm forming factor. a microbial corrosion as a result of interaction between the biofilm and metal surface was considered. the interaction was displayed in biomineralization. the future trends of biofilms study were bound with research of their architecture. that architecture was determined by the structure and function of biofilms compounds: biopolyme ...201020695231
prevalence of antimicrobial resistance among gram-negative isolates in an adult intensive care unit at a tertiary care center in saudi arabia.patients in the icu have encountered an increasing emergence and spread of antibiotic-resistant pathogens. we examined patterns of antimicrobial susceptibility in gram-negative isolates to commonly used drugs in an adult icu at a tertiary care hospital in riyadh, saudi arabia.201020697174
[isolation, identification, and degrading characteristics of a high-efficient pyrene-degrading bacterial strain].by using selective enrichment culture with pyrene as the sole carbon source, a pyrene-degrading bacterial strain zq5 was isolated from the oil-contaminated soil in shenfu irrigation area. the strain was identified as stenotrophomonas sp., based on its morphological, physiological, and biochemical characteristics, and similarity identification of 16s rdna sequence. the pyrene-degrading characteristics and the effects of culture condition on the degrading efficiency of the strain were investigated ...201020707122
infections occurring during extracorporeal membrane oxygenation use in adult patients.the application of extracorporeal membrane oxygenation in adults has been increasing, but infections occurring during extracorporeal membrane oxygenation use are rarely described.201020708754
biocidal efficacy of silver-impregnated contact lens storage cases in vitro.silver-impregnated contact lens (cl) storage cases are designed to reduce microbial contamination during use, but there are limited data on their effectiveness. this study evaluated early antimicrobial activity of silver-impregnated cl cases and silver-release characteristics in vitro.201120720221
stenotrophomonas maltophilia endocarditis of prosthetic mitral valve.we present a 78-year-old woman with prosthetic valve endocarditis due to stenotrophomonas maltophilia (s. maltophilia) 3 years after mitral valve replacement. administration of sulfamethoxazole-trimethoprim was effective; however, it was discontinued because of side effects, which led to failure of antibiotic therapy. complications of multiple cerebral infarction and paravalvular abscess developed. although the prosthetic valve was removed 50 days after admission, she died 4 months after surgery ...201020720357
anaerobic oxidation of arsenite linked to chlorate reduction.microorganisms play a significant role in the speciation and mobility of arsenic in the environment. in this study, the oxidation of arsenite [as(iii)] to arsenate [as(v)] linked to chlorate (clo₃⁻) reduction was shown to be catalyzed by sludge samples, enrichment cultures (ecs), and pure cultures incubated under anaerobic conditions. no activity was observed in treatments lacking inoculum or with heat-killed sludge, or in controls lacking clo₃⁻. the as(iii) oxidation was linked to the complete ...201020729322
a cluster of acute-onset postoperative endophthalmitis over a 1-month period: investigation of an outbreak caused by uncommon species.to report the clinical course, treatment response and prognosis of eight cases which developed acute-onset postoperative endophthalmitis over a 1-month period.201120733020
treatment of recurrent stenotrophomonas maltophilia ventilator-associated pneumonia with doxycycline and aerosolized colistin.to report a case of recurrent stenotrophomonas maltophilia ventilator-associated pneumonia (vap) that was successfully treated with doxycycline and aerosolized colistin.201020736426
diversity of cold-active protease-producing bacteria from arctic terrestrial and marine environments revealed by enrichment culture.a new approach for enrichment culture was applied to obtain cold-active protease-producing bacteria for marine and terrestrial samples from svalbard, norway. the method was developed for the enrichment of bacteria by long-term incubation at low temperatures in semi-solid agar medium containing meat pieces as the main source of carbon and energy. zobell and 0.1x nutrient broth were added for marine and terrestrial microorganisms, respectively, to supply basal elements for growth. one to three typ ...201020799082
stenotrophomonas maltophilia as a causal agent of pyogranulomatous hepatitis in a buffalo (bubalus bubalis).a 7-year-old female buffalo (bubalus bubalis) from a local herd in serres, northern greece, was presented to a private veterinary clinic with a chronic loss of appetite for 15 days. the clinical examination revealed high fever (41.5 degrees c), lethargy, yellow discoloration of skin and mucous membranes, an abdomen that appeared to be empty, hyperactive rumen motility, and tachypnea. a biochemical profile revealed an elevated total bilirubin concentration and hepatic enzyme activities, whereas g ...201020807941
[taxonomic position of certain representatives of sulphate-reducing corrosive microbial community].sulfate-reducing bacteria and their heterotrophic satellites have been isolated from the biofilm formed on steel by sulfidogenic corrosive microbial community. bacteria were characterized according to phenotypical features and investigated by the methods of molecular-genetic analysis. in accordance with the phenotypical features the studied strain of sulfate-reducing bacteria were related to desulfovibrio genus, gram-positive strains of heterotrophic satellites were related to bacillus genus, b. ...201020812503
[physiological properties and taxonomic position of soil microorganisms-destructors of hexachlorcyclohexane].eleven (11) microbial strains capable to decompose insecticide hexachlorcyclohexane (hch) have been isolated from microbial association resistant to chloro-organic pesticides. on the basis of investigation of morphological, cultural, physiological, biochemical characteristics and results of sequence of gene 16s rrna it has been established, that the best strain-destructors n 3 and n 9 belong to pseudomonas putida species, and strain n 6 belongs to stenotrophomonas maltophilia species.201020812504
[diversity of bacteria associated with pine wood nematode revealed by metagenome].the pine wood nematode (pwn), bursaphlenchus xylophilus, which collaborates with its associated bacteria to form ecosystem and has interaction among them, is the pathogen of pine wilt disease. this study focused on revealing the bacterial diversity of ecosystem of pine wood nematode and its associated bacteria.201020815238
trimethoprim/sulfamethoxazole pharmacokinetics in two patients undergoing continuous venovenous hemodiafiltration.to determine the extent of elimination of trimethoprim (tmp) and sulfamethoxazole (smx) via continuous venovenous hemodiafiltration (cvvhdf) in 2 critically ill patients with renal failure.201020823279
cloning, purification, crystallization and preliminary x-ray diffraction of the olec protein from stenotrophomonas maltophilia involved in head-to-head hydrocarbon biosynthesis.olec, a biosynthetic enzyme involved in microbial hydrocarbon biosynthesis, has been crystallized. synchrotron x-ray diffraction data have been collected to 3.4 a resolution. the crystals belonged to space group p3(1)21 or p3(2)21, with unit-cell parameters a=b=98.8, c=141.0 a.201020823539
medical and surgical management of severe inflammation of the nictitating membrane in a giant panda (ailuropoda melanoleuca).a 10-year-old male giant panda (ailuropoda melanoleuca) presented for severe, acute swelling and protrusion of the right nictitating membrane, unresponsive to topical therapy. excisional biopsy of the nictitating membrane and its associated lacrimal gland was elected due to necrosis and friability of the tissue. histopathology revealed suppurative, necrotizing conjunctivitis and dacryoadenitis. culture grew stenotrophomonas maltophila and enterococcus spp with extensive antibiotic resistance. tr ...201020840099
extended multidrug-resistant stenotrophomonas maltophilia septicemia in a severely burnt patient. 201020852428
hexavalent chromium reduction by bacterial consortia and pure strains from an alkaline industrial effluent.to characterize the bacterial consortia and isolates selected for their role in hexavalent chromium removal by adsorption and reduction.201020854455
exploring characteristics of bioelectricity generation and dye decolorization of mixed and pure bacterial cultures from wine-bearing wastewater treatment.this study uncovered microbial characteristics of bioelectricity generation and dye decolorization in single-chamber microbial fuel cells (mfcs) using activated sludge for wine-containing wastewater treatment. phylogenetic tree analysis on 16s rrna gene fragments indicated that the predominant strains on anodic biofilm in acclimatized mfcs were gamma-proteobacteria aeromonas punctata niu-p9, pseudomonas plecoglossicida niu-y3, pseudomonas koreensis niu-x8, acinetobacter junii niu-y8, stenotropho ...201120859654
evaluation of the diversilab system for detection of hospital outbreaks of infections by different bacterial species.many bacterial typing methods are specific for one species only, time-consuming, or poorly reproducible. diversilab (dl; biomérieux) potentially overcomes these limitations. in this study, we evaluated the dl system for the identification of hospital outbreaks of a number bacterial species. appropriately typed clinical isolates were tested with dl. dl typing agreed with pulsed-field gel electrophoresis (pfge) for acinetobacter (n = 26) and stenotrophomonas maltophilia (n = 13) isolates. with two ...201020861340
c-terminal truncation of a metagenome-derived detergent protease for effective expression in e. coli.recently, a new alkaline protease named hp70 showing highest homology to extracellular serine proteases of stenotrophomonas maltophilia and xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (niehaus et al., submitted for publication). attempts to efficiently express the enzyme in common expression hosts had failed. this study reports on the realization of overexpression in escherichia coli after structural modification of hp70. modelling of hp70 res ...201020869404
[clinical strains isolation and antibiotic susceptibility of stenotrophomonas maltophilia].stenotrophomonas maltophilia is an opportunistic gram-negative bacillus which is becoming increasingly recognized as an important nosocomial pathogen especially in debilitated or immune suppressed patients. s. maltophilia is found in a wide variety of environments. it has been isolated from a number of water sources, soil, variety of plants and food sources. s. maltophilia can form biofilm on synthetic materials for temporary or permanent implantation, i.e. central venous catheters, urinary cath ...201020873485
endophthalmitis caused by stenotrophomonas maltophilia.the authors investigate clinical settings, antibiotic susceptibility and resistance patterns, and visual outcomes associated with endophthalmitis caused by stenotrophomonas maltophilia.201020873692
honeydew honey as a potent antibacterial agent in eradication of multi-drug resistant stenotrophomonas maltophilia isolates from cancer patients.multi-drug resistance in nosocomial pathogens is a continually evolving and alarming problem in health care units. since ancient times, honey has been used successfully for the treatment of a broad spectrum of infections with no risk of resistance development. this study investigated the antibacterial activity of two natural honeys, namely honeydew and manuka, against 20 nosocomial multi-drug resistant stenotrophomonas maltophilia (s. maltophilia) isolates from cancer patients. an antibiotic sus ...201020882522
stenotrophomonas maltophilia in cystic fibrosis: serologic response and effect on lung disease.stenotrophomonas maltophilia is one of the more common multidrug-resistant organisms isolated from the respiratory tract of patients with cystic fibrosis (cf), but the effect of chronic s. maltophilia infection on cf lung disease is unknown.201020889901
bacterial stimulation of copper phytoaccumulation by bioaugmentation with rhizosphere bacteria.copper contaminated areas pose environmental health risk to living organisms. remediation processes are thus required for both crop production and industrial activities. this study employed bioaugmentation with copper resistant bacteria to improve phytoremediation of vineyard soils and copper mining waste contaminated with high copper concentrations. oatmeal plant (avena sativa l.) was used for copper phytoextraction. three copper resistant bacterial isolates from oatmeal rhizosphere (pseudomona ...201020937516
bacterial contamination of stock solutions in storage cases for contact lens, and the disinfectant-resistance of isolates.to investigate the bacterial contamination of preservative solutions for contact lenses, contact lens cases were swabbed and the swabs were cultured. various bacteria were isolated from 26 of 100 samples (26.0%). by preservative solution, the bacterial presence was low (16.7%) in the 30 samples from cases using renu, which was the most frequently employed solution, and 27.3% in 11 samples from cases using complete, which was the second most frequently employed solution. of 34 strains isolated an ...201020938091
discrepancies between various methods in susceptibility testing and epidemiological analysis of stenotrophomonas maltophilia clinical isolates.the susceptibility of 25 stenotrophomonas maltophilia (s. maltophilia) clinical isolates to four different antimicrobials (trimethoprim/sulfomethoxazole, piperacillin/tazobactam, ceftazidime, ciprofloxacin) were investigated by disk diffusion, e-test and commercial sensititre and pasco broth microdilution techniques. discrepancies between the results of broth microdilution and the other methods studied were characterized as very major, major and minor errors. using the broth microdilution as the ...201020939265
mimicking disinfection and drying of biofilms in contaminated endoscopes.the effects of peracetic acid-based (paa) disinfectant with, and without, additional drying on candida albicans, candida parapsilosis, pseudomonas aeruginosa and stenotrophomonas maltophilia, isolated from contaminated flexible endoscopes, in single- and dual-species biofilms were studied. biofilms were prepared in sterile tissue culture polystyrene 96-well microtitre plates and were quantified using the tetrazolium salt (mtt) reduction assay and by counting colony-forming yeasts and bacteria fr ...201020951470
stenotrophomonas maltophilia strains from cystic fibrosis patients: genomic variability and molecular characterization of some virulence determinants.the genetic relatedness of 52 stenotrophomonas maltophilia strains, collected from various environmental and clinical sources, including cystic fibrosis (cf) patients, as well as the presence and the expression of some virulence-associated genes were studied. pulsed-field gel electrophoresis (pfge) analysis identified 47 profiles and three clusters of isolates with an identical pfge pattern considered to be indistinguishable strains. restriction fragment length polymorphism of the gyrb gene grou ...201020952251
chloramphenicol and rifampin may be the only options against stenotrophomonas maltophilia. a tale of a colonized bladder device in a patient with myelofibrosis. 201020956878
comparative studies on toluene removal and pressure drop in biofilters using different packing materials.to select the best available packing material for malodorous organic gases such as toluene and benzene, biofilter performance was compared in biofilters employed different packing materials including porous ceramic (celite), jeju scoria (lava), a mixture of granular activated carbon (gac) and celite (gac/celite), and cubic polyurethane foam (pu). a toluene-degrading bacterium, stenotrophomonas maltophilia t3-c, was used as the inoculum. the maximum elimination capacities in the celite, lava, and ...201021047004
[etiology and epidemic characteristics of hospital acquired pneumonia in children].to investigate the etiology and epidemic characteristics of hospital acquired pneumonia (hap) in children.201021055271
metallo-beta-lactamase production by pseudomonas otitidis: a species-related trait.susceptibility to several β-lactams and β-lactamase production was investigated in a collection of 20 strains of pseudomonas otitidis, a new pseudomonas species that has been recently recognized in association with otic infections in humans. all strains appeared to be susceptible to piperacillin, cefotaxime, ceftazidime, and aztreonam, while resistance or decreased susceptibility to carbapenems was occasionally observed. all strains were found to express metallo-β-lactamase (mbl) activity and to ...201021060106
acanthamoeba and stenotrophomonas maltophilia keratitis with fungal keratitis in the contralateral eye.the purpose of this study is to describe the diagnosis, course, and outcome of a case of acanthamoeba and stenotrophomonas keratitis with a fungal keratitis in the contralateral eye.201021060673
characteristics and consequences of airway colonization by filamentous fungi in 201 adult patients with cystic fibrosis in france.a total of 657 sputum samples from 201 cystic fibrosis adult patients were collected during a 24-month period (2005-2006). we retrospectively analyzed the fungal colonization of the respiratory tract of these individuals by linking medical records and microbiological data. filamentous fungi were isolated from specimens of 65.6% of the patients, with aspergillus fumigatus being the predominant species recovered as it was found in specimens of 56.7% of the patients. we observed no difference for g ...201021067327
tetracycline resistance associated with commensal bacteria from representative ready-to-consume deli and restaurant foods.proper knowledge of antibiotic resistance (ar) dissemination is essential for effective mitigation. this study examined the profiles of tetracycline-resistant (tetr) commensal bacteria from representative ready-to-consume food samples from salad bars at local grocery stores and restaurants. out of 900 tetr isolates examined, 158 (17.6%) carried one or more of tetm, tetl, tets, and tetk genes by conventional pcr, 28 harbored more than one tetr determinants. the most prevalent genotype was tetm, w ...201021067672
bacterial diversity, organic pollutants and their metabolites in two aeration lagoons of common effluent treatment plant (cetp) during the degradation and detoxification of tannery wastewater.in this study, pcr-rflp and gc-ms approaches were used to characterize the bacterial diversity, organic pollutants and metabolites during the tannery wastewater treatment process at common effluent treatment plant (cetp). results revealed that the bacterial communities growing in aeration lagoon-i were dominated with escherichia sp., stenotrophomonas sp., bacillus sp. and cronobacter sp. while that of aeration lagoon-ii prevailed with stenotrophomonas sp., and burkholderiales bacterium, respecti ...201021075615
[a clinical analysis of 25 cases of prosthetic valve endocarditis].to report the clinical characteristics of prosthetic valve endocarditis (pve).201021092446
two new variants of and creation of a repository for stenotrophomonas maltophilia quinolone protection protein (smqnr) genes. 201021093222
pathogen-host interactions in dictyostelium, legionella, mycobacterium and other pathogens.dictyostelium discoideum is a haploid social soil amoeba that is an established host model for several human pathogens. the research areas presently pursued include the use of d. discoideum to identify genetic host factors determining the outcome of infections and the use as screening system for identifying bacterial virulence factors. here we report about the legionella pneumophila directed phagosome biogenesis and the cell-to-cell spread of mycobacterium species. moreover, we highlight recent ...201021109012
challenging and emerging pathogens in cystic fibrosis.cystic fibrosis (cf) lung disease is characterised by chronic inflammation and infection. patients are predominantly infected by specific pathogens, of which staphylococcus aureus and pseudomonas aeruginosa are the most important. in recent years however there has been an increasing number of reports on potentially emerging and challenging pathogens like stenotrophomonas maltophilia, non-tuberculous mycobacteria, highly prevalent p. aeruginosa clones, methicillin resistant staphylococcus aureus ...201021109184
analysis of heat-induced changes in protein expression of stenotrophomonas maltophilia k279a reveals a role for groel in the host-temperature adaptation.stenotrophomonas maltophilia is a microorganism of environmental and clinical importance as well as a frequent airway colonizer of cystic fibrosis (cf) individuals. we combined 2-de and maldi-tof ms to profile the protein expression in s. maltophilia k279a, a completely sequenced clinical isolate, grown at 37 °c with respect to the strain grown at 26 °c. among the proteins up-regulated at 37 °c, we identified groel, a molecular chaperone that mainly assist the folding and unfolding of proteins u ...201021112247
prolonged venovenous extracorporeal membrane oxygenation in a child with leukemia and persistent bacteremia.objective:: in patients who require extracorporeal membrane oxygenation for prolonged periods, it is uncertain whether nosocomial bacteremia that persists throughout an entire extracorporeal membrane oxygenation run can be associated with good outcomes. design:: case report. setting:: tertiary pediatric intensive care unit. patient:: a 6-yr-old boy with acute myeloid leukemia and prolonged mechanical ventilatory support. interventions:: venovenous extracorporeal membrane oxygenation. measurement ...201021116210
degradative potential of stenotrophomonas strain hpc383 having genes homologous to dmp operon.a strain, stenotrophomonas hpc383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through hplc and gc analysis. culture hpc383 could degrade (%) various compounds (1 mm) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% c ...201021123060
immunological and pathological aspects of respiratory tract infection with stenotrophomonas maltophilia in balb/c mice.a comprehensive study on the production of inflammatory mediators in the lungs of balb/c mice following infection with stenotrophomonas maltophilia was conducted. the levels of pro-inflammatory cytokines, tumor necrosis factor alpha (tnf-alpha) and interleukin-1beta (il-1 beta) was raised in the lungs of infected mice compared to control. the production of anti-inflammatory cytokine il-10 was slightly delayed. its peak level was on 2nd day whereas the peak of pro-inflammatory cytokines was obser ...201021124066
optimization of culture media for enhanced chitinase production from a novel strain of stenotrophomonas maltophilia using response surface methodology.chitinase is one of the most important mycolytic enzymes with industrial significance. this enzyme is produced by a number of organisms including bacteria. in this study we describe optimization of media components with increased production of chitinase for selected bacteria stenotrophomonas maltophilia isolated from the soil. different components of the defined media responsible for influencing chitinase secretion by the bacterial isolate were screened using plackett-burman experimental design ...201021124068
new perspectives on immunomodulatory therapy for bacteraemia and sepsis.systemic immune dysregulation is generally acknowledged to be the fundamental molecular mechanism that underlies the pathophysiology of severe sepsis and septic shock. in the presence of a systemic infection, microbial pathogens and their soluble mediators induce generalised immune activation and coagulation activation, leading to severe sepsis and septic shock. for decades, immune-based therapies have been devised with the specific intent of inhibiting the pro-inflammatory events that are thoug ...201021129935
[the growing problem of antibiotic resistance in clinically relevant gram-negative bacteria: current situation].resistance to antimicrobial agents in clinically relevant gram-negative bacteria is an increasingly important problem, which in the last few years has spread from the hospital setting to the community. in enterobacteria, the main features of this situation include resistance to β-lactams and quinolones. β-lactam resistance is caused by intrinsic β-lactamases, extended-spectrum β-lactamases, plasmid-mediated cephamycinases and carbapenemses, particularly when produced in strains with decreased pe ...201021130927
abundance and diversity of biofilms in natural and artificial aquifers of the äspö hard rock laboratory, sweden.six cores were drilled and retrieved from 186-m depth in the äspö hard rock laboratory (hrl) tunnel to investigate whether indigenous biofilms develop on fracture surfaces in groundwater-conducting aquifers in granitic rock. a clone library was constructed from fracture surface material (fsm), for community composition analysis. quantitative polymerase chain reaction (qpcr) was applied to quantify gene copies using the 16s rrna gene for domain bacteria and the adenosine-phosphosulfate reductase ...201021132427
diversity of bacterial endophytes in roots of mexican husk tomato plants (physalis ixocarpa) and their detection in the rhizosphere.endophytic bacterial diversity was estimated in mexican husk tomato plant roots by amplified rdna restriction analysis and sequence homology comparison of the 16s rdna genes. sixteen operational taxonomic units from the 16s rdna root library were identified based on sequence analysis, including the classes gammaproteobacteria, betaproteobacteria, actinobacteria, and bacilli. the predominant genera were stenotrophomonas (21.9%), microbacterium (17.1%), burkholderia (14.3%), bacillus (14.3%), and ...201021157706
[risk factors for ventilator-associated pneumonia in neonates and the changes of isolated pathogens.]objective: to study the risk factors for neonatal ventilator-associated pneumonia (vap) and the changes of isolated pathogens in the last eight years. methods: the clinical data of 230 neonates who were admitted into the neonatal intensive care unit (nicu) and received mechanical ventilation for equal to or longer than 48 hrs in 2008 were retrospectively reviewed. the isolated pathogens were compared with those of eight years ago. results: the incidence of vap (25.2%) in the year 2008 was lower ...201021172126
late-onset intrascleral dissemination of stenotrophomonas maltophilia scleritis after pterygium excision.to report a case of pterygium excision-related infectious scleritis with late-onset intrascleral dissemination.201121173698
dacryocystitis secondary to stenotrophomonas maltophilia infection.an 88-year-old man developed recurrent bouts of dacryocystitis and underwent dacryocystorhinostomy. cultures from the lacrimal sac demonstrated stenotrophomonas maltophilia. this uncommon bacterium is highly antibiotic resistant, and clinicians should consider it in atypical cases of infection and should guide antibiotic therapy appropriately.201021178795
co-existence of staphylococcus spp and stenotrophomonas maltophilia infection in persistent endophthalmitis after cataract surgery. 201021182662
biodegradation of fenvalerate and 3-phenoxybenzoic acid by a novel stenotrophomonas sp. strain zs-s-01 and its use in bioremediation of contaminated soils.a bacterial strain zs-s-01, newly isolated from activated sludge, could effectively degrade fenvalerate and its hydrolysis product 3-phenoxybenzoic acid (3-pba). based on the morphology, physiological biochemical characteristics, and 16 s rdna sequence, strain zs-s-01 was identified as stenotrophomonas sp. strain zs-s-01 could also degrade and utilize deltamethrin, beta-cypermethrin, beta-cyfluthrin, and cyhalothrin as substrates for growth. strain zs-s-01 was capable of degrading fenvalerate ra ...201021184062
reassessment of stenotrophomonas maltophilia phenotype.standard microbiology references describe stenotrophomonas maltophilia as oxidase negative and variable with respect to utilization of lactose and sucrose. analysis of a collection of 766 s. maltophilia isolates indicated that approximately 20% are oxidase positive and that this species should be reevaluated for other phenotypes, including oxidative fermentation of lactose and sucrose.201021191048
genetic and phenotypic diversity of plant growth promoting rhizobacteria isolated from sugarcane plants growing in pakistan.bacteria were isolated from roots of sugarcane varieties grown in the fields of punjab. they were identified by using api20e/ne bacterial identification kits and from sequences of 16s rrna and amplicons of the cpn60 gene. the majority of bacteria were found to belong to the genera of enterobacter, pseudomonas, and klebsiella, but members of genera azospirillum, rhizobium, rahnella, delftia, caulobacter, pannonibacter, xanthomonas, and stenotrophomonas were also found. the community, however, was ...201021193815
conversion of oleic acid to 10-hydroxystearic acid by whole cells of stenotrophomonas nitritireducens.the optimal reaction conditions for the conversion of oleic acid to 10-hydroxystearic acid by whole cells of stenotrophomonas nitritireducens were: ph 7.5, 35 °c, 0.05% (w/v) tween 80, 20 g cells l(-1), and 30 g oleic acid l(-1) in an anaerobic atmosphere. under these conditions, the cells produced 31.5 g 10-hydroxystearic acid l(-1) over 4 h with a conversion yield of 100% (mol/mol) and a productivity of 7.9 g l(-1) h(-1), indicating that oleic acid was converted completely to 10-hydroxystearic ...201121207107
decreased mucosal oxygen tension in the maxillary sinuses in patients with cystic fibrosis.pseudomonas aeruginosa in the sinuses plays a role in the lungs in cystic fibrosis (cf) patients, but little is known about the sinus environment where the bacteria adapt. anoxic areas are found in the lower respiratory airways but it is unknown if the same conditions exist in the sinuses.201121208831
[in vitro antimicrobial activity of piperacillin-sulbactam and other 7 antimicrobial agents against non-fermentative bacilli].to evaluate the in vitro antimicrobial activity of piperacillin-sulbactam against clinical isolates of non-fermentative bacilli isolated from common infections.201021211358
efficacy of an anaerobic swab transport system to maintain aerobic and anaerobic microorganism viability after storage at -80 degrees c.an amies agar gel swab transport system was evaluated for its ability to maintain bacterial viability and relative quantity after freezing at -80°c. nine american type culture collection (atcc) bacterial strains were used: 3 anaerobic strains (propionibacterium acnes, peptostreptococcus anaerobius, and clostridium sporogenes) and 6 facultative or strict aerobic bacterial strains (stenotrophomonas maltophilia, escherichia coli ([atcc 25922 and atcc 11775], salmonella enterica subsp. enterica sero ...201121217035
secretion, modification, and regulation of ax21.innate immunity provides a first line of defense against pathogen attack and is activated rapidly following infection. although it is now widely appreciated that host receptors of conserved microbial signatures play a key role in innate immunity in plants and animals, very little is known about the biological function of the microbially derived molecules recognized by such receptors. we have recently demonstrated that the rice xa21 receptor binds the axy(s)22 peptide corresponding to the n-termi ...201121236725
in vitro and in vivo properties of bal30376, a β-lactam and dual beta-lactamase inhibitor combination with enhanced activity against gram-negative bacilli that express multiple β-lactamases.bal30376 is a triple combination comprising a siderophore monobactam, bal19764; a novel bridged monobactam, bal29880, which specifically inhibits class c β-lactamases; and clavulanic acid, which inhibits many class a and some class d β-lactamases. the mic(90) was ≤ 4 μg/ml (expressed as the concentration of bal19764) for most species of the enterobacteriaceae family, including strains that produced metallo-β-lactamases and were resistant to all of the other β-lactams tested. the mic(90) for sten ...201121245441
delineation of stenotrophomonas spp. by multi-locus sequence analysis and maldi-tof mass spectrometry.the genus stenotrophomonas is genetically and phenotypically heterogeneous. of the nine species now accepted, only s. maltophilia is of clinical importance. based on dna-sequences of seven house keeping genes, it encompasses genogroups of dna-similarity below 97% that predominantly comprise strains of environmental origin. therefore, in order to unravel the uneven distribution of environmental isolates within genogroups and reveal genetic relationships within the genus, there is need for an easy ...201121247714
[isolation and identification of a polyhydroxyalkanoate producing strain].we screened and isolated polyhydroxyalkanoate producing bacteria.201021268894
influence of referral bias on the clinical characteristics of patients with gram-negative bloodstream infection.summaryreferral bias can influence the results of studies performed at tertiary-care centres. in this study, we evaluated demographic and microbiological factors that influenced referral of patients with gram-negative bloodstream infection (bsi). we identified 2919 and 846 unique patients with gram-negative bsi in a referral cohort of patients treated at mayo clinic hospitals and a population-based cohort of olmsted county, minnesota, residents between 1 january 1998 and 31 december 2007, respec ...201121281552
antithymocyte globulin-induced acute lung injury during transplantation for aplastic anemia.a 10-year-old boy with acquired, very severe aplastic anemia developed acute lung injury after the administration of equine antithymocyte globulin, during conditioning for allogenic bone marrow transplantation. limited cases of antithymocyte globulin-induced acute lung injury have been described in adults. the respiratory worsening was sudden and required mechanical ventilation. the clinical course was complicated by sepsis with escherichia coli, vancomycin-resistant enterococci, and stenotropho ...201121285902
count, identification and antimicrobial susceptibility of bacteria recovered from dental solid waste in brazil.in brazil, few studies on microbial content of dental solid waste and its antibiotic susceptibility are available. an effort has been made through this study to evaluate the hazardous status of dental solid waste, keeping in mind its possible role in cross-infection chain. six samples of solid waste were collected at different times and seasons from three dental health services. the microbial content was evaluated in different culture media and atmospheric conditions, and the isolates were submi ...201121288707
stenotrophomonas maltophilia resistance to trimethoprim/sulfamethoxazole mediated by acquisition of sul and dfra genes in a plasmid-mediated class 1 integron.stenotrophomonas maltophilia is becoming a more and more common cause of infections. in this study, the minimal inhibitory concentrations of trimethoprim/sulfamethoxazole (sxt), ceftazidime, minocycline, levofloxacin, chloramphenicol and ticarcillin/clavulanic acid were determined and the distribution of integrons and sul1, sul2 and dfra genes was investigated in 102 s. maltophilia isolates collected from patients treated in 31 hospitals in anhui, china, in the month of september in 2006-2008. t ...201121296557
virulence of environmental stenotrophomonas maltophilia serologically cross-reacting with shigella-specific antisera.this research involved an environmental strain of stenotrophomonas maltophilia which has been reported to produce serological cross-reactivity with shigella dysenteriae type 8 specific antisera. since clinical diagnosis of shigellosis is largely based on culture and serology, the investigation was aimed at in vivo and in vitro virulence comparison between the culturally similar environmental s. maltophilia isolate and the reference s. dysenteriae strains. the findings of this study revealed the ...201021313916
rapid genotyping of achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia isolates using melting curve analysis of rapd-generated dna fragments (mcrapd).typing of bacteria is important for monitoring newly emerging pathogens and for examining local outbreaks. we evaluated the randomly amplified polymorphic dna technique in combination with melting curve analysis (mcrapd) of the amplified dna fragments to genotype isolates from five gram-negative species, i.e. achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia. by determining the melting temperature peaks of the ampl ...201121320595
isolation of a selected microbial consortium capable of degrading methyl parathion and p-nitrophenol from a contaminated soil site.a bacterial consortium with the ability to degrade methyl parathion and p-nitrophenol, using these compounds as the only carbon source, was obtained by selective enrichment in a medium with methyl parathion. samples were taken from moravia, medellin; an area that is highly contaminated, owing to the fact that it was used as a garbage dump from 1974 to 1982. acinetobacter sp, pseudomonas putida, bacillus sp, pseudomonas aeruginosa citrobacter freundii, stenotrophomonas sp, flavobacterium sp, prot ...201121328125
long term performance of an arsenite-oxidizing-chlorate-reducing microbial consortium in an upflow anaerobic sludge bed (uasb) bioreactor.a chlorate (clo(3)(-)) reducing microbial consortium oxidized arsenite (as(iii)) to arsenate (as(v)) in an upflow anaerobic sludge-bed bioreactor over 550 days operation. as(iii) was converted with high conversion efficiencies (>98%) at volumetric loadings ranging from 0.45 to 1.92 mmol as/(l(reactor)d). the oxidation of as(iii) was linked to the complete reduction of clo(3)(-) to cl(-) and h(2)o, as demonstrated by a molar ratio of approximately 3.0 mol as(iii) oxidized per mole of cl(-) formed ...201121333531
screening and identification of a novel esterase estpe from a metagenomic dna library.esterases represent a large family of hydrolases with broad substrate specificity and functional sequence space. although many attempts to screen new esterases have been conducted, there have been few reports conducted to discriminate unique enzymes from typical ones based on novel structure and function. in this study, we discovered an esterase and a novel family through a successive assay of whole cells and crude lysates (oxidative open condition). the screened putative esterases from the meta ...201121369973
[study of mutagenic activity of hexachlorocyclohexane and products of its microbial degradation].mutagenic activity of hexachlorcyclohexane (hch) was investigated. as is known, gamma-isomer of hch has insecticidal properties. the strains of microorganisms of genera pseudomonas, stenotrophomonas, bacillus decomposing hch into 46.3-86.7% from initial quantity have been selected. microbial hch destruction by investigated strains made changes of pesticide genetic activity: in most cases in condition of intermediate action the quantity of mutations s. typhimurium ta 100 and s. typhimurium ta 98 ...201021381312
the third-generation p-glycoprotein inhibitor tariquidar may overcome bacterial multidrug resistance by increasing intracellular drug concentration.the use of efflux pump inhibitors may be a powerful strategy to overcome transporter-mediated bacterial multidrug resistance. in the present study, we set out to investigate the potency of tariquidar, a third-generation p-glycoprotein inhibitor in clinical development, for overcoming bacterial resistance towards ciprofloxacin.201121393173
smqnrr, a deor-type transcriptional regulator, negatively regulates the expression of smqnr and smtcra in stenotrophomonas maltophilia.to characterize the role of smqnrr in the expression of smqnr and smtcra, and the role of smtcra in drug resistance in stenotrophomonas maltophilia.201121393212
in vitro activity of ceftaroline against gram-positive and gram-negative pathogens isolated from patients in canadian hospitals in 2009.the in vitro activities of ceftaroline and comparative agents were determined for a collection of the most frequently isolated bacterial pathogens from hospital-associated patients across canada in 2009 as part of the ongoing canward surveillance study. in total, 4,546 isolates from 15 sentinel canadian hospital laboratories were tested using the clinical and laboratory standards institute (clsi) broth microdilution method. compared with other cephalosporins, including ceftobiprole, cefepime, an ...201121402844
pme-1, an extended-spectrum ß-lactamase identified in pseudomonas aeruginosa.a novel extended-spectrum ß-lactamase (esbl) was identified in a pseudomonas aeruginosa clinical isolate obtained from a patient admitted to a hospital in pennsylvania in 2008. the patient had a prolonged hospitalization in a hospital in dubai, united arab emirates, before being transferred to the united states. the novel esbl, designated pme-1 (pseudomonas aeruginosa esbl 1), is a molecular class a, bush-jacoby-medeiros group 2be enzyme and shared 50, 43, and 41% amino acid identity with the l2 ...201121402845
[biodiversity of mesophilic microbial community bynd-8 capability of lignocellulose degradation and its effect on biogas production].the biodiversity of a mesophilic microbial community bynd-8 capable of degrading lignocellulose at 30 degrees c was detected using denaturing gradient gel electrophoresis (dgge) and the isolation of pure cultures, and the effect of the liquid of rice straw degradation by bynd-8 on biogas production was measured. six bacterial strains were isolated using peptone cellulose solution medium, and the highest similarities of their 16s rdna gene sequences to serratia sp. psgb 13, s. marcescens strain u ...201121404695
adhesion of stenotrophomonas maltophilia to mouse tracheal mucus is mediated through flagella.adhesion of stenotrophomonas maltophilia, an opportunistic pathogen, to different surfaces has been reported in the literature. however, its ability to adhere to mucus and the involvement of different bacterial appendages in this process has not been elucidated. in this study, bacterial adhesion to mouse tracheal mucus as well as the role of flagella in the adhesion process were investigated using clinical isolates of s. maltophilia. all the clinical isolates adhered to mouse tracheal mucus to v ...201121415208
separation of the bacterial species, escherichia coli, from mixed-species microbial communities for transcriptome analysis.the study of bacterial species interactions in a mixed-species community can be facilitated by transcriptome analysis of one species in the community using cdna microarray technology. however, current applications of microarrays are mostly limited to single species studies. the purpose of this study is to develop a method to separate one species, escherichia coli as an example, from mixed-species communities for transcriptome analysis.201121418656
application of a continuously stirred tank bioreactor (cstr) for bioremediation of hydrocarbon-rich industrial wastewater effluents.a continuously stirred tank bioreactor (cstr) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. a successful bioremediation was developed by an efficient acclimatized microbial consortium. after an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. the performance of the bioaugmented reactor was demonstrated by the reduction of cod rates up to 95%. the residual tota ...201121419572
acute obstructive suppurative pancreatic ductitis. 201121421076
correlation between antibiotic consumption and resistance of gram-negative bacteria causing healthcare-associated infections at a university hospital in taiwan from 2000 to 2009.this study investigated the correlation between antibiotic consumption and antimicrobial resistance in gram-negative bacteria causing healthcare-associated infections at a university hospital in taiwan from 2000 to 2009.201121436153
monitoring the bacterial community of maize silage stored in a bunker silo inoculated with enterococcus faecium, lactobacillus plantarum and lactobacillus buchneri.to monitor variations in the bacterial community and fermentation products of maize silage within and between bunker silos.201121447012
clinical and microbiologic outcomes in trauma patients treated for stenotrophomonas maltophilia ventilator-associated pneumonia.to determine clinical and microbiologic plus clinical success rates in critically ill trauma patients who received treatment for stenotrophomonas maltophilia ventilator-associated pneumonia (vap).201121449623
[the microbiology laboratory's contribution to the surveillance and control of outbreaks caused by nonfermentative gram-negative bacilli].pseudomonas aeruginosa, acinetobacter baumannii and stenotrophomonas maltophilia are, among others, the most important nonfermentative gram-negative bacilli within hospitals. these organisms are able to cause different types of nosocomial infections in predisposed patients, and p. aeruginosa and a. baumannii in particular may cause extensive clonal outbreaks and complex situations in which sporadic isolates may coexist with some epidemic strains. some common features are their intrinsic resistan ...201121458710
gastrointestinal selective capacity of doripenem, meropenem, and imipenem for carbapenem-resistant gram-negative bacilli in treated patients with pneumonia. 201121460501
causative pathogen dictates optimal duration of antimicrobial therapy for ventilator-associated pneumonia in trauma patients.recent ventilator-associated pneumonia (vap) guidelines recommend considering abbreviated therapy in patients with non-pseudomonas aeruginosa vap if clinical signs resolve. however, using an arbitrary day cutoff or clinical signs can be suboptimal for some, especially multiply injured patients, resulting in relapse and/or antibiotic resistance. previously, we showed that repeat bronchoalveolar lavage (bal) could guide antimicrobial duration for community-acquired vap in trauma patients. the purp ...201121463773
chronic pulmonary infection with stenotrophomonas maltophilia and lung function in patients with cystic fibrosis.the clinical consequences of chronic stenotrophomonas maltophilia infection in cystic fibrosis (cf) patient are still unclear.201121463972
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