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ticks and associated pathogens collected from cats in sicily and calabria (italy).limited information is available about the species of ticks infesting the cat and the pathogens that they harbor. the aims of the present study were to identify the species of ticks removed from cats living in sicily and calabria (italy) and to detect dna of vector-borne pathogens in the same ticks.201526445916
establishing the discriminating concentration for permethrin and fipronil resistance in rhipicephalus sanguineus (latreille) (acari: ixodidae), the brown dog tick.rhipicephalus sanguineus (latreille), the brown dog tick, is a veterinary canine and urban pest. these ticks have been found to develop resistance and tolerance to the two commonly used acaricides permethrin and fipronil respectively. we have developed a discriminating concentration that can be used for rapid detection of permethrin and fipronil resistance in brown dog tick populations. the availability of a discriminating concentration for the brown dog tick provides an inexpensive and rapid re ...201626449370
successful isolation of leishmania infantum from rhipicephalus sanguineus sensu lato (acari: ixodidae) collected from naturally infected dogs.the main transmission route of leishmania infantum is through the bites of sand flies. however, alternative mechanisms are being investigated, such as through the bites of ticks, which could have epidemiological relevance. the objective of this work was to verify the presence of leishmania spp. in rhipicephalus sanguineus sensu lato collected from naturally infected dogs in the federal district of brazil.201526452666
emerging tick-borne infections in mainland china: an increasing public health threat.since the beginning of the 1980s, 33 emerging tick-borne agents have been identified in mainland china, including eight species of spotted fever group rickettsiae, seven species in the family anaplasmataceae, six genospecies in the complex borrelia burgdorferi sensu lato, 11 species of babesia, and the virus causing severe fever with thrombocytopenia syndrome. in this review we have mapped the geographical distributions of human cases of infection. 15 of the 33 emerging tick-borne agents have be ...201526453241
repellency and acaricidal efficacy of a new combination of fipronil and permethrin against ixodes ricinus and rhipicephalus sanguineus ticks on dogs.a blinded, controlled laboratory study was conducted to assess the repellency and acaricidal activity of a topical spot on formulation, a combination of fipronil and permethrin, against ixodes ricinus and rhipicephalus sanguineus ticks on dogs.201526463998
frequency and clinical epidemiology of canine monocytic ehrlichiosis in dogs infested with ticks from sinaloa, mexico.ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. the prevalence of this disease in veterinary medicine can vary depending on the diagnostic method used and the geographic location. one hundred and fifty-two canine blood samples from six veterinary clinics and two shelters from sinaloa state (mexico) were analyzed in this study. all animals were suspected of having canine monocytic ehrlichiosis (cme). the diagnostic methods use ...201326464910
a survey of tick-borne pathogens in dogs and their ticks in the pantanal biome, brazil.tick and blood samples collected from domestic dogs in the brazilian pantanal were tested by molecular methods for the presence of tick-borne protozoa and bacteria. among 320 sampled dogs, 3.13% were infected by babesia vogeli (piroplasmida: babesiidae), 8.75% by hepatozoon canis (eucoccidiorida: hepatozoidae), 7.19% by anaplasma platys (rickettsiales: anaplasmataceae), and 0.94% by an unclassified anaplasma sp. in three tick species collected from dogs, the following tick-borne agents were dete ...201626467462
candidatus neoehrlichia sp. in an austrian fox is distinct from candidatus neoehrlichia mikurensis, but closer related to candidatus neoehrlichia lotoris.candidatus neoehrlichia came under the focus of recent research in terms of human and pet relevance. candidatus neoehrlichia mikurensis seems to be relatively abundant in animals and humans from central european countries, whereas candidatus neoehrlichia lotoris was found solely in raccoons from the usa.201526471191
emerging and re-emerging zoonoses of dogs and cats.since the middle of the 20th century, pets are more frequently considered as "family members" within households. however, cats and dogs still can be a source of human infection by various zoonotic pathogens. among emerging or re-emerging zoonoses, viral diseases, such as rabies (mainly from dog pet trade or travel abroad), but also feline cowpox and newly recognized noroviruses or rotaviruses or influenza viruses can sicken our pets and be transmitted to humans. bacterial zoonoses include bacter ...201426480316
comparative analysis of spermatids of rhipicephalus sanguineus sensu lato (ixodidae) and ornithodoros rostratus ticks (argasidae): morphophysiology aimed at systematics.the phylogenetic relationships among tick species (acari: ixodida) have been revisited by several researchers over the last decades. two subfamilies, rhipicephalinae (ixodidae) and ornithodorinae (argasidae), deserve special attention. the male reproductive system morphology, as well as the ultrastructure of the germ cells, may provide important information for phylogeny and systematics of metazoan groups, with spermatozoa exhibiting characters that can be used for this purpose. with that inform ...201626481487
seroprevalence survey of babesia gibsoni infection and tick species in dogs in east china.a seroprevalence survey of babesia gibsoni infection in dogs in east china was conducted using an elisa with recombinant b. gibsoni thrombospondin-related adhesive protein (bgtrap). a total of 1170 dogs from east china were examined and the seroprevalence was 9.23%. the proportion of samples was 81.2%, 7.86% and 10.94% from pet, working and fighting dogs, respectively. the fighting dogs showed highest seroprevalence (39.8%) compared with working dogs (26.1%) and pet dogs (3.47%). these results i ...201526483164
vector-borne diseases in stray dogs in peninsular malaysia and molecular detection of anaplasma and ehrlichia spp. from rhipicephalus sanguineus (acari: ixodidae) ticks.little data are available on the prevalence and transmission of vector-borne diseases in stray dogs in peninsular malaysia. this study was designed to determine the occurrence of vector-borne pathogens in malaysian stray dogs using serological and molecular approaches. in total, 48 dog blood samples were subjected to serological analysis using snap 4dx kit (idexx laboratories, westbrook, me). the presence of ehrlichia and anaplasma dna in the dog blood samples and rhipicephalus sanguineus (latre ...201626494821
expanding range of amblyomma americanum and simultaneous changes in the epidemiology of spotted fever group rickettsiosis in the united states.spotted fever group (sfg) rickettsia species are etiologic agents of a wide range of human infections from asymptomatic or mild infections to severe, life-threatening disease. in the united states, recent passive surveillance for sfg rickettsiosis shows an increased incidence and decreased severity of reported cases. the reasons for this are not well understood; however, we hypothesize that less pathogenic rickettsiae are causing more human infections, while the incidence of disease caused by mo ...201526503270
molecular detection of rickettsia conorii and other zoonotic spotted fever group rickettsiae in ticks, romania.the diverse tick fauna as well as the abundance of tick populations in romania represent potential risks for both human and animal health. spotted fever group (sfg) rickettsiae are recognized as important agents of emerging human tick-borne diseases worldwide. however, the epidemiology of rickettsial diseases has been poorly investigated in romania. in urban habitats, companion animals which are frequently exposed to tick infestation, play a role in maintenance of tick populations and as reservo ...201626507182
detection of tick-borne pathogens in the korean water deer (hydropotes inermis argyropus) from jeonbuk province, korea.the objective of this study was to investigate the prevalence of tick-borne pathogens in the korean water deer (hydropotes inermis argyropus). pathogens were identified using pcr which included anaplasma, ehrlichia, rickettsia, and theileria. rickettsia was not detected, whereas anaplasma, ehrlichia, and theileria infections were detected in 4, 2, and 8 animals, respectively. the most prevalent pathogen was theileria. of the 8 theileria-positive animals, 2 were mixed-infected with 3 pathogens (a ...201526537046
bacteria associated with amblyomma cajennense tick eggs.ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. in the americas, the ixodidae ticks amblyomma cajennense are responsible for severe impact on livestock and public health. in the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with a. cajennense eggs from females sampled in distinct geographical sites in southeastern brazil. additional comparative analysis of the culturable bacteria from an ...201526537602
systematic revision of the adeleid haemogregarines, with creation of bartazoon n. g., reassignment of hepatozoon argantis garnham, 1954 to hemolivia, and molecular data on hemolivia stellata.life cycles and molecular data for terrestrial haemogregarines are reviewed in this article. collection material was re-examined: hepatozoon argantis garnham, 1954 in argas brumpti was reassigned to hemolivia as hemolivia argantis (garnham, 1954) n. comb.; parasite dna was extracted from a tick crush on smear of an archived slide of hemolivia stellata in amblyomma rotondatum, then the 18s ssrrna gene was amplified by pcr. a systematic revision of the group is proposed, based on biological life c ...201526551414
ectoparasite infestations and canine infection by rickettsiae and ehrlichiae in a semi-arid region of northeastern brazil.this study investigated the prevalence of rickettsia spp. and ehrlichia canis infection in dogs and their ectoparasites from rural and urban areas of two municipalities, petrolina and juazeiro, within a semiarid region (caatinga biome) of northeastern brazil, by immunofluorescence assay (ifa) and polymerase chain reaction (pcr). overall, 12.1% (61/504) and 23.0% (116/504) of canine plasma samples had antibodies reactive to rickettsia spp. and e. canis. e. canis dna was detected by pcr in 8.3% (4 ...026565771
molecular detection of emerging tick-borne pathogens in vojvodina, serbia.ticks play an important role in disease transmission globally due to their capability to serve as vectors for human and animal pathogens. the republic of serbia is an endemic area for a large number of tick-borne diseases. however, current knowledge on these diseases in serbia is limited. the aim of this study was to investigate the presence of new emerging tick-borne pathogens in ticks collected from dogs and the vegetation from different parts of vojvodina, serbia. a total of 187 ticks, includ ...201626565929
molecular evidence of spotted fever group rickettsiae and anaplasmataceae from ticks and stray dogs in bangladesh.emerging tick-borne diseases (tbds) are important foci for human and animal health worldwide. however, these diseases are sometimes over looked, especially in countries with limited resources to perform molecular-based surveys. the aim of this study was to detect and characterize spotted fever group (sfg) rickettsiae and anaplasmataceae in bangladesh, which are important tick-borne pathogens for humans and animals worldwide. a total of 50 canine blood samples, 15 ticks collected from dogs, and 1 ...201626573516
detection of canine vector-borne diseases in eastern poland by elisa and pcr.the aim of the study was to establish the prevalence of ehrlichia canis, anaplasma phagocytophilum and borrelia burgdorferi in dogs in eastern poland and to determine the factors associated with exposure (seroposity) or infection (pcr). anti-a. phagocytophilum, anti-b. burgdorferi and anti-e. canis antibodies were determined in 400 dogs, using the snap 4dx ® test (idexx laboratories). in addition, pcrs were performed for the detection of e. canis, a. phagocytophilum and b. burgdorferi dna. in re ...201526581374
a novel combination of fipronil and permethrin (frontline tri-act®/frontect®) reduces risk of transmission of babesia canis by dermacentor reticulatus and of ehrlichia canis by rhipicephalus sanguineus ticks to dogs.the ability of frontline tri-act®/frontect®, a topical ectoparasiticide containing fipronil and permethrin for dogs, to prevent the transmission of babesia canis as well as ehrlichia canis was evaluated by infesting dogs with infected vector ticks.201526586365
satellite hyperspectral imagery to support tick-borne infectious diseases surveillance.this study proposed the use of satellite hyperspectral imagery to support tick-borne infectious diseases surveillance based on monitoring the variation in amplifier hosts food sources. to verify this strategy, we used the data of the human rickettsiosis occurrences in southeastern brazil, region in which the emergence of this disease is associated with the rising capybara population. spatio-temporal analysis based on monte carlo simulations was used to identify risk areas of human rickettsiosis ...201526599337
emerging horizons for tick-borne pathogens: from the 'one pathogen-one disease' vision to the pathobiome paradigm.ticks, as vectors of several notorious zoonotic pathogens, represent an important and increasing threat for human and animal health in europe. recent applications of new technology revealed the complexity of the tick microbiome, which may affect its vectorial capacity. appreciation of these complex systems is expanding our understanding of tick-borne pathogens, leading us to evolve a more integrated view that embraces the 'pathobiome'; the pathogenic agent integrated within its abiotic and bioti ...201526610021
first detection of ehrlichia canis in cerebrospinal fluid from a nonthrombocytopenic dog with meningoencephalitis by broad-range pcr. 201526610407
rickettsia rickettsii and rickettsia felis infection in rhipicephalus sanguineus ticks and ctenocephalides felis fleas co-existing in a small city in yucatan, mexico. 201526611982
dinotefuran-induced morphophysiological changes in the ovaries and midgut of semi-engorged females rhipicephalus sanguineus latreille, 1806 (acari: ixodidae) ticks.the present study demonstrated the effects of dinotefuran (active compound of the protetor pet® acaricide) in germ cells and the digestive processes of semi-engorged females of r. sanguineus exposed to different concentrations of the chemical. for this purpose, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group i or control (distilled water), group ii (5000 ppm), group iii (6250 ppm), and group iv (8334 ppm of dinotefuran). all ticks were immersed i ...201626614361
molecular identification and characterization of anaplasma platys and ehrlichia canis in dogs in mexico.the tick-borne pathogens ehrlichia canis and anaplasma platys are the causative agents of canine monocytic ehrlichiosis (cme) and canine cyclic thrombocytopenia (cct). although molecular evidence of e. canis has been shown, phylogenetic analysis of this pathogen has not been performed and a. platys has not been identified in mexico, where the tick vector rhipicephalus sanguineus sensu lato (s.l.) is common. the aim of this research was to screen, identify and characterize e. canis and a. platys ...201626615872
rickettsia rickettsii isolation from naturally infected amblyomma parvum ticks by centrifugation in a 24-well culture plate technique.rocky mountain spotted fever is an acute illness caused by rickettsia rickettsii (r. rickettsii) and is transmitted by the bite of ticks of the genera dermacentor, amblyomma and rhipicephalus. the illness results in a high mortality rate and may be easily confused with other febrile syndromes. in yucatan state, mexico, childhood cases with a high mortality have been reported. in this work we report the isolation of a mexican r. rickettsii strain from a tick egg mass using an alternative method f ...201326623321
vectors of crimean congo hemorrhagic fever virus in iran.ticks are important vectors and reservoirs of crimean congo hemorrhagic fever (cchf) virus. human beings may be infected whenever the normal life cycle of the infected ticks on non-human vertebrate hosts is interrupted by the undesirable presence of humans in the cycle. a total of 26 species of argasid and ixodid ticks have been recorded in iran; including nine hyalomma, two rhipicephalus, two dermacentor, five haemaphysalis, two boophilus, one ixodes and two argas as well as three ornithodoros ...201526623426
seroprevalence and risk factors of ehrlichia canis infection among companion dogs of mashhad, north east of iran, 2009-2010.the aims of this study were to determine the seroprevalence of canine ehrlichiosis and risk factors of this disease in companion dogs' population of mashhad, north east of iran. canine monocytic ehrlichiosis (cme) is a zoonotic disease transmitted by ticks, rhipicephalus sanguineus, and caused by an obligate intracellular bacterium, ehrlichia canis.201526623430
targeted silencing of the aquaporin 2 gene of rhipicephalus (boophilus) microplus reduces tick fitness.ticks are blood-feeding arthropods that can affect human and animal health both directly by blood-feeding and indirectly by transmitting pathogens. the cattle tick rhipicephalus (boophilus) microplus is one of the most economically important ectoparasites of bovines worldwide and it is responsible for the transmission of the protozoan babesia bovis, the etiological agent of bovine babesiosis. aquaporins (aqps) are water channel proteins implicated in physiological mechanisms of osmoregulation. m ...201526626727
morphology, epidemiology, and phylogeny of babesia: an overview.babesiosis is a tick-borne hemoprotozoan disease of domestic and wild animals. the disease is caused by various species of babesia and some species of babesia have also zoonotic significance. the parasite in vertebrate hosts' remains in erythrocytes and the morphology of babesia spp. is not uniform in all vertebrate hosts. with the advancement of science, particularly the use of molecular techniques made it easy to study the evolution of parasites and thereby reclassifying babesia spp. as per th ...026629451
the first detection of rickettsia aeschlimannii and rickettsia massiliae in rhipicephalus turanicus ticks, in northwest china.rickettsia spp. belonging to the spotted fever group (sfg) cause infections in humans, domestic animals and wildlife. at least five sfg rickettsial species have been reported in china, but the occurrence of rickettsia aeschlimannii and r. massiliae in ticks has not been characterized to date.201526652857
molecular screening for rickettsia, anaplasmataceae and coxiella burnetii in rhipicephalus sanguineus ticks from malaysia.a total of 44 rhipicephalus sanguineus ticks collected from 23 dogs from malaysia were screened for rickettsia, anaplasmataceae and coxiella burnetii. coxiella burnetii was detected in 59% (26/44) of ticks however rickettsia and anaplasmataceae were not detected in any of the ticks. in order to genotype the strains of c. burnetii, multispacer sequence typing (mst) was carried out using three different spacers. one of the spacers; cox2 successfully amplified a fragment for which the full length s ...201526691268
multiplex pcr for molecular screening of borrelia burgdorferi sensu lato, anaplasma spp. and babesia spp.ticks transmit a great variety of pathogenic microorganisms to humans and animals. the detection of tick-borne pathogens (tbp) is mainly by molecular techniques based on polymerase chain reactions (pcr).201526706969
tick-borne rickettsial pathogens in questing ticks, removed from humans and animals in mexico.tick-borne rickettsial diseases (tbrd) are commonly encountered in medical and veterinary clinical settings. the control of these diseases is difficult, requiring disruption of a complex transmission chain involving a vertebrate host and ticks. the geographical distribution of the diseases is related to distribution of the vector, which is an indicator of risk for the population. a total of 1107 were collected by tick drag from forests, ecotourism parks and hosts at 101 sites in 22 of the 32 sta ...026726019
[factors associated with ehrlichia canis infection in dogs infested with ticks from huanuco, peru].the aim of the study was to determine the frequency and associated factors of ehrlichia canis infection in dogs. blood samples from 150 dogs infested with ticks in 10 veterinary clinics in the city of huanuco in peru were collected. the dogs were randomly selected without regard to breed, age or sex. ehrlichia canis antibodies were detected by chromatographic immunoassay.51.3% of dogs were infected with ehrlichia canis. in the multivariate analysis, factors associated with the presence of ehrlic ...201526732926
seroepidemiological studies of crimean-congo hemorrhagic fever virus in domestic and wild animals.crimean-congo hemorrhagic fever (cchf) is a widely distributed, tick-borne viral disease. humans are the only species known to develop illness after cchf virus (cchfv) infection, characterized by a nonspecific febrile illness that can progress to severe, often fatal, hemorrhagic disease. a variety of animals may serve as asymptomatic reservoirs of cchfv in an endemic cycle of transmission. seroepidemiological studies have been instrumental in elucidating cchfv reservoirs and in determining endem ...201626741652
ixodes scapularis tick saliva proteins sequentially secreted every 24 h during blood feeding.ixodes scapularis is the most medically important tick species and transmits five of the 14 reportable human tick borne disease (tbd) agents in the usa. this study describes lc-ms/ms identification of 582 tick- and 83 rabbit proteins in saliva of i. scapularis ticks that fed for 24, 48, 72, 96, and 120 h, as well as engorged but not detached (bd), and spontaneously detached (sd). the 582 tick proteins include proteases (5.7%), protease inhibitors (7.4%), unknown function proteins (22%), immunity ...201626751078
molecular detection of crimean-congo haemorrhagic fever virus in ticks collected from infested livestock populations in a new endemic area, south of iran.crimean-congo haemorrhagic fever (cchf) is a viral zoonotic disease with potentially fatal systemic effects on man. we aimed to determine the presence of cchf virus among collected ticks from domestic livestock from october 2012 to september 2013.201626758985
molecular detection and identification of spotted fever group rickettsiae in ticks collected from the west bank, palestinian territories.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group (sfg) rickettsiae. although spotted fever is prevalent in the middle east, no reports for the presence of tick-borne pathogens are available or any studies on the epidemiology of this disease in the west bank. we aimed to identify the circulating hard tick vectors and genetically characterize sfg rickettsia species in ixodid ticks from the west bank-palestinian territories.201626771654
molecular, biological, and morphometric comparisons between different geographical populations of rhipicephalus sanguineus sensu lato (acari: ixodidae).in this study, different geographical populations of rhipicephalus sanguineus sensu lato were compared by molecular, biological, and morphometric methods. phylogenetic trees were constructed using 12s and 16s rdna sequences and showed two distinct clades: one composed of ticks from brazil (jaboticabal, sp), cuba (havana) thailand (bangkok) and the so-called "tropical strain" ticks. the second clade was composed of ticks from spain (zaragoza), argentina (rafaela, santa fe) and the so-called "temp ...201626790741
detection of bartonella tamiae, coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern algeria.in recent years, the scope and importance of emergent vector-borne diseases has increased dramatically. in algeria, only limited information is currently available concerning the presence and prevalence of these zoonotic diseases. for this reason, we conducted a survey of hematophagous ectoparasites of domestic mammals and/or spleens of wild animals in el tarf and souk ahras, algeria.201626791781
refractory hypoglycaemia in a dog infected with trypanosoma congolense.a 20 kg german shepherd dog was presented to a french veterinary teaching hospital for seizures and hyperthermia. the dog had returned 1 month previously from a six-month stay in senegal and sub-saharan africa. biochemistry and haematology showed severe hypoglycaemia (0.12 g/l), anaemia and thrombocytopenia. despite administration of large amounts of glucose (30 ml of 30% glucose iv and 10 ml of 70% sucrose by gavage tube hourly), 26 consecutive blood glucose measurements were below 0.25 g/l (ex ...201626795063
assessment of the prophylactic speed of kill of frontline tri-act(®) against ticks (ixodes ricinus and rhipicephalus sanguineus) on dogs.the objective of the study was to assess the speed of kill of a single topical treatment with a combination of fipronil and permethrin (frontline tri-act(®)/frontect(®)) against experimental infestations of ixodes ricinus and rhipicephalus sanguineus ticks on dogs. in this parallel group designed, randomised, single centre, controlled efficacy study, 16 healthy adult dogs were allocated to two groups: 8 dogs were treated with the topical combination on day 0 and the other 8 dogs served as untrea ...201626795064
first detection and molecular identification of babesia vogeli from rhipicephalus sanguineus (acari: ixodidae) in taiwan.a total of 578 rhipicephalus sanguineus ticks collected from dogs in taiwan were examined for babesia by species-specific polymerase chain reaction assay based on the 18s small subunit ribosomal rna (ssrrna) gene. babesia dna was detected in 1.04 % (6/578) of rh. sanguineus ticks. phylogenetic analysis revealed that these babesia spp. were genetically linked to the same clade within the genospecies of babesia vogeli and could be discriminated from other genospecies of babesia. intra-species anal ...201626796569
update on canine and feline blood donor screening for blood-borne pathogens.an update on the 2005 american college of veterinary internal medicine (acvim) consensus statement on blood donor infectious disease screening was presented at the 2015 acvim forum in indianapolis, indiana, followed by panel and audience discussion. the updated consensus statement is presented below. the consensus statement aims to provide guidance on appropriate blood-borne pathogen testing for canine and feline blood donors in north america.201626806261
fatal babesia canis canis infection in a splenectomized estonian dog.a previously splenectomized dog from estonia was presented with a sudden lack of appetite and discoloration of the urine. despite supportive therapy, its condition deteriorated dramatically during 1 day. severe thrombocytopenia and high numbers of protozoan hemoparasites were evident in blood smears, and the hematocrit dropped from 46 to 33 %. the dog was euthanized before specific antibabesial treatment was initiated. blood samples from the dog and from two other dogs in the same household test ...201626810086
frequency and distribution of rickettsiae, borreliae, and ehrlichiae detected in human-parasitizing ticks, texas, usa.to describe the presence and distribution of tickborne bacteria and their vectors in texas, usa, we screened ticks collected from humans during 2008-2014 for rickettsia, borrelia, and ehrlichia spp. thirteen tick species were identified, and 23% of ticks carried bacterial dna from at least 1 of the 3 genera tested.026811941
candidatus coxiella massiliensis infection.bacteria genetically related to coxiella burnetii have been found in ticks. using molecular techniques, we detected coxiella-like bacteria, here named candidatus coxiella massiliensis, in skin biopsy samples and ticks removed from patients with an eschar. this organism may be a common agent of scalp eschar and neck lymphadenopathy after tick bite.201626811945
knock-down and speed of kill of a combination of fipronil and permethrin for the prevention of ctenocephalides felis flea infestation in dogs.a topical combination of fipronil + permethrin (frontline tri-act/frontect, merial) has recently been developed to control fleas, ticks, mosquitoes, sandflies and stable flies on dogs. two studies were conducted to assess its speed of kill and knock-down effect on ctenocephalides felis fleas. the combination was compared to either fipronil alone or to a combination of permethrin, dinotefuran, and pyriproxyfen,201626830931
[epidemiological investigation of predominance tick and the infectious status of severe fever thrombocytopenia syndrome virus in penglai and laizhou counties, shandong province].to investigate the predominance ticks and the infectious status of severe fever with thrombocytopenia (sftsv) in penglai and laizhou counties, shandong province.201526833011
climate change, biodiversity, ticks and tick-borne diseases: the butterfly effect.we have killed wild animals for obtaining food and decimated forests for many reasons. nowadays, we are burning fossil fuels as never before and even exploring petroleum in deep waters. the impact of these activities on our planet is now visible to the naked eye and the debate on climate change is warming up in scientific meetings and becoming a priority on the agenda of both scientists and policy decision makers. on the occasion of the impact of environmental changes on infectious diseases (iec ...201526835253
growth of ehrlichia canis, the causative agent of canine monocytic ehrlichiosis, in vector and non-vector ixodid tick cell lines.canine monocytic ehrlichiosis is caused by ehrlichia canis, a small gram-negative coccoid bacterium that infects circulating monocytes. the disease is transmitted by the brown dog tick rhipicephalus sanguineus s.l. and is acknowledged as an important infectious disease of dogs and other members of the family canidae worldwide. e. canis is routinely cultured in vitro in the canine monocyte-macrophage cell line dh82 and in non-vector ixodes scapularis tick cell lines, but not in cells derived from ...201626837859
the putative role of rhipicephalus microplus salivary serpins in the tick-host relationship.inflammation and hemostasis are part of the host's first line of defense to tick feeding. these systems are in part serine protease mediated and are tightly controlled by their endogenous inhibitors, in the serpin superfamily (serine protease inhibitors). from this perspective ticks are thought to use serpins to evade host defenses during feeding. the cattle tick rhipicephalus microplus encodes at least 24 serpins, of which rms-3, rms-6, and rms-17 were previously identified in saliva of this ti ...201626844868
morphological alterations in salivary glands of rhipicephalus sanguineus ticks (acari: ixodidae) exposed to neem seed oil with known azadirachtin concentration.neem (azadirachta indica) has attracted the attention of researchers worldwide due to its repellent properties and recognized effects on the morphology and physiology of arthropods, including ticks. therefore, this study aimed to demonstrate the effects of neem seed oil enriched with azadirachtin on salivary glands of rhipicephalus sanguineus ticks, targets of great veterinary interest because of their ability to transmit pathogens to dogs. for this, r. sanguineus semi-engorged females were subj ...201626852009
genomic insights into the ixodes scapularis tick vector of lyme disease.ticks transmit more pathogens to humans and animals than any other arthropod. we describe the 2.1 gbp nuclear genome of the tick, ixodes scapularis (say), which vectors pathogens that cause lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. the large genome reflects accumulation of repetitive dna, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. annotation of scaffolds representing ∼57% of the ge ...201626856261
origin and evolution of rickettsial plasmids.rickettsia species are strictly intracellular bacteria that have undergone a reductive genomic evolution. despite their allopatric lifestyle, almost half of the 26 currently validated rickettsia species have plasmids. in order to study the origin, evolutionary history and putative roles of rickettsial plasmids, we investigated the evolutionary processes that have shaped 20 plasmids belonging to 11 species, using comparative genomics and phylogenetic analysis between rickettsial, microbial and no ...201626866478
a brown dog tick (rhipicephalus sanguineus) bite at sea.medical officers (mos) and medical branch ratings (mbrs) must maintain an awareness of the risk of vector-borne diseases among deployed personnel. personnel working in the maritime environment may not expect to be at risk of tick bites, as ticks typically occupy habitats with dense vegetation such as forests or scrub land. however, tick-borne pathogens can cause serious and sometimes fatal disease, and therefore the risk of tick bites and associated diseases should be recognised. we present a ca ...201526867423
brazilian spotted fever with an approach in veterinary medicine and one health perspective.there is increasing interaction between man and pathogens transmitted by arthropods, especially by ticks. it is on this background that a holistic approach stands out, for the sake of public health. brazilian spotted fever is an endemic disease at the country's southeast, with amblyomma sculptum as its major contributor, followed by a. aureolatum and potentially rhipicephalus sanguineus. dogs have been considered sentinels, and in some areas the disease in dogs can precede human disease. conside ...201626881183
ticks associated with domestic dogs and cats in florida, usa.voluntary collections of ticks from domestic dogs and cats by veterinary practitioners across florida, usa, were conducted over a 10 month period. of the 1337 ticks submitted, five species of ixodid ticks were identified and included rhipicephalus sanguineus, amblyomma americanum, a. maculatum, dermacentor variabilis, and ixodes scapularis. most ticks were collected from dogs (98.4%) with the most predominant species being r. sanguineus (94.3%). of the ticks collected from cats (1.6%), a. americ ...201626888081
comparative speed of kill of sarolaner (simparica) and afoxolaner (nexgard) against induced infestations of rhipicephalus sanguineus s.l. on dogs.the brown dog tick, rhipicephalus sanguineus sensu lato, commonly infests dogs globally, is the major vector of the pathogen that causes canine monocytic ehrlichiosis and also transmits babesia vogeli. a rapid speed of kill of a parasiticide is essential to reduce the direct deleterious effects of tick infestation and the risk of tick-borne pathogen transmission. the speed of kill of a novel orally administered isoxazoline parasiticide, sarolaner (simparica), against r. sanguineus sensu lato on ...201626896456
comparative speed of kill of sarolaner (simparica) and afoxolaner (nexgard) against induced infestations of amblyomma americanum on dogs.the lone star tick, amblyomma americanum, infests dogs and cats in north america and is the vector of the pathogens that cause monocytic and granulocytic ehrlichiosis in dogs and humans. a parasiticide's speed of kill is important to minimize the direct and deleterious effects of tick infestation and especially to reduce the risk of transmission of tick-borne pathogens. in this study, speed of kill of a novel orally administered isoxazoline parasiticide, sarolaner (simparica chewable tablets), a ...201626897175
missed retained tick: "look for the legs". 201526900234
arsenophonus nasoniae and rickettsiae infection of ixodes ricinus due to parasitic wasp ixodiphagus hookeri.arsenophonus nasoniae, a male-killing endosymbiont of chalcid wasps, was recently detected in several hard tick species. following the hypothesis that its presence in ticks may not be linked to the direct occurrence of bacteria in tick's organs, we identified a. nasoniae in wasps emerging from parasitised nymphs. we confirmed that 28.1% of ixodiphagus hookeri wasps parasitizing ixodes ricinus ticks were infected by a. nasoniae. moreover, in examined i. ricinus nymphs, a. nasoniae was detected on ...201626901622
comparative speed of kill of oral treatments with simparica™ (sarolaner) and bravecto®(fluralaner) against induced infestations of rhipicephalus sanguineus on dogs.rhipicephalus sanguineus is the most widely distributed tick species infesting dogs worldwide, which may cause discomfort to the host and transmit diseases. acaricides with a rapid and sustained speed of kill are thus important to prevent infestation and to reduce the risk of disease transmission. in this study, the speed of kill of a monthly administered simparica™ (sarolaner) treatment against induced infestations with r. sanguineus on dogs was evaluated and compared with a single dose of brav ...201626911244
transcriptome of larvae representing the rhipicephalus sanguineus complex.rhipicephalus sanguineus sensu lato (ixodida: ixodidae) is possibly the most widespread tick species worldwide, responsible for transmitting several vector-borne pathogens of medical and veterinary importance. here, we explore the transcriptome of r. sanguineus s.l. larvae (putignano strain). we sequenced total rna from r. sanguineus s.l. larvae. a total of 15,566,986 short paired-end reads were de novo-assembled into 33,396 transcripts and then annotated and analyzed. particular attention was p ...201726921517
prediction of g protein-coupled receptor encoding sequences from the synganglion transcriptome of the cattle tick, rhipicephalus microplus.the cattle tick, rhipicephalus (boophilus) microplus, is a pest which causes multiple health complications in cattle. the g protein-coupled receptor (gpcr) super-family presents a candidate target for developing novel tick control methods. however, gpcrs share limited sequence similarity among orthologous family members, and there is no reference genome available for r. microplus. this limits the effectiveness of alignment-dependent methods such as blast and pfam for identifying gpcrs from r. mi ...201626922323
novel phleboviruses detected in ticks, greece.since 2009, when severe fever with thrombocytopenia syndrome virus and heartland virus have been identified and associated with disease in humans, the interest on tick-borne phleboviruses is increasing rapidly. the aim of the present study was to investigate the presence of tick-borne phleboviruses in greece and compare them with respective ones detected worldwide. ticks collected from goats and sheep in 60 sites of 13 regional units of greece were grouped in pools (1-3 ticks per pool) and teste ...201626935112
efficacy of a novel oral formulation of sarolaner (simparica™) against five common tick species infesting dogs in the united states.the efficacy of a single oral treatment with sarolaner (simparica™, zoetis), a novel isoxazoline compound, was evaluated against five tick species known to infest dogs in the united states. a total of 10 laboratory studies, two against each species, were conducted using adult purpose-bred mongrels or beagle dogs. in each study, 16 dogs were randomly allocated to one of two treatment groups based on pre-treatment host-suitability tick counts. dogs were infested with approximately 50 unfed adult a ...201626935819
hierarchical bayesian spatio-temporal analysis of climatic and socio-economic determinants of rocky mountain spotted fever.this study aims to examine the spatio-temporal dynamics of rocky mountain spotted fever (rmsf) prevalence in four contiguous states of midwestern united states, and to determine the impact of environmental and socio-economic factors associated with this disease. bayesian hierarchical models were used to quantify space and time only trends and spatio-temporal interaction effect in the case reports submitted to the state health departments in the region. various socio-economic, environmental and c ...201626942604
molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from china evaluated by nested pcrs.an investigation was performed to detect eight pathogens in ticks collected from grass tips or animals in the southern, central and northeast regions of china. dna samples extracted from ticks were collected from ten different locations in eight provinces of china and subjected to screening for tick-borne pathogens, including borrelia burgdorferi sensu lato, ehrlichia spp., rickettsia spp., babesia/theileria spp., ehrlichia ruminantium, coxiella burnetii, and francisella tularensis, using nested ...201626943995
antifungal, antiradical and cytotoxic activities of extractives obtained from tagetes patula l. (asteraceae), a potential acaricide plant species.tagetes patula l. shows a complex chemical composition, ranging from glycosylated flavonoids and thiophenes in extracts until terpenoids in the essential oil. in the present study, due to this rich flavonoidic constitution, its antioxidant potential was determined, having shown values of antiradical percentage superior to reference compounds, mainly the extracts prepared with flowers. previous studies performed emphasized the acaricide potential of t. patula and thus, the present study aimed to ...201626945559
determination of the effective dose of a novel oral formulation of sarolaner (simparica™) for the treatment and month-long control of fleas and ticks on dogs.three laboratory studies were conducted to determine the appropriate dose of sarolaner, a novel isoxazoline, for the treatment and month-long control of infestations of fleas and ticks on dogs. in the first study, dogs were treated orally with sarolaner suspension formulations at 1.25, 2.5 or 5.0mg/kg, and infested with dermacentor reticulatus, rhipicephalus sanguineus ticks and with ctenocephalides felis felis (cat flea) prior to treatment and then weekly for up to 8 weeks. fleas and ticks were ...201626948830
sero-epidemiological survey of crimean-congo hemorrhagic fever virus in tunisia.crimean-congo hemorrhagic fever (cchf) is a tick-borne disease associated with a high case fatality rate and transmitted mainly by hyalomma marginatum. the geographical distribution of h. marginatum covers most of the western mediterranean basin. we aimed to investigate whether cchf virus (cchfv) is circulating in tunisia. samples from unexplained acute febrile patients (n = 181) and a high risk group of humans, mainly slaughter workers (n = 38), were collected in the summer of 2014 and analyzed ...201626956221
serological and molecular investigation of ehrlichia spp. and anaplasma spp. in ticks and blood of dogs, in the thrace region of turkey.in recent years, tick-borne diseases like ehrlichiosis and anaplasmosis became widespread worldwide threatening the health of both human and companion animals. therefore, the aim of this study was to determine the presence of anaplasma spp., and ehrlichia spp. in dogs and ticks in the thrace region of turkey. a total of 400 blood samples and 912 ticks were collected from dogs living in shelters that are located in four cities (istanbul, edirne, tekirdag and kirklareli) of the thrace region. bloo ...201626961274
discovery of sarolaner: a novel, orally administered, broad-spectrum, isoxazoline ectoparasiticide for dogs.the novel isoxazoline ectoparasiticide, sarolaner, was identified during a lead optimization program for an orally-active compound with efficacy against fleas and ticks on dogs. the aim of the discovery program was to identify a novel isoxazoline specifically for use in companion animals, beginning with de novo synthesis in the zoetis research laboratories. the sarolaner molecule has unique structural features important for its potency and pharmacokinetic (pk) properties, including spiroazetidin ...201626961590
novel spotted fever group rickettsiae in haemaphysalis qinghaiensis ticks from gansu, northwest china.rickettsia spp. are obligate intracellular bacteria and well known as transmitted by arthropods. these pathogens have a broad geographic distribution and a high degree of biological and clinical diversity. this study was conducted to determine the prevalence and molecular characterization of rickettsia spp. in ticks collected from gansu, where borrelia burgdorferi sensu lato and anaplasma phagocytophilum were previously reported in ticks and ruminants.201626968160
molecular detection of theileria, babesia, and hepatozoon spp. in ixodid ticks from palestine.ixodid ticks transmit various infectious agents that cause disease in humans and livestock worldwide. a cross-sectional survey on the presence of protozoan pathogens in ticks was carried out to assess the impact of tick-borne protozoa on domestic animals in palestine. ticks were collected from herds with sheep, goats and dogs in different geographic districts and their species were determined using morphological keys. the presence of piroplasms and hepatozoon spp. was determined by pcr amplifica ...201626969489
molecular identification of borrelia genus in questing hard ticks from portugal: phylogenetic characterization of two novel relapsing fever-like borrelia sp.in the last decades, several studies have reported pathogenic species of borrelia related to those that cause tick-borne relapsing fever (rf), but unexpectedly suggesting their transmission by hard ticks, known vectors of borrelia burgdorferi sensu lato (b. burgdorferi s.l.) species, rather than by soft ticks. this study was designed to update the presence of b. burgdorferi s.l. species in ticks from several districts of mainland portugal, where ixodes ricinus had been previously described. tick ...201626976475
genetic diversity and molecular characterization of babesia motasi-like in small ruminants and ixodid ticks from china.ovine babesioses, an important tick-borne disease of sheep and goats in china, is caused by the reproduction of intraerythrocytic protozoa of the babesia genus. babesia motasi-like is a babesia parasite that infects small ruminant in china, and two sub-groups of b. motasi-like can be subdivided based on differences in the rhoptry-associated-protein-1 gene. this study aimed to characterize the distribution, epidemiology and genetics of b. motasi-like in animals and ticks. a molecular investigatio ...201626976477
molecular detection of anaplasma platys infection in free-roaming dogs and ticks from kenya and ivory coast.anaplasma platys is a bacterium parasitic in the canine platelets, representing the causative agent of canine cyclic thrombocytopenia, with a worldwide distribution, mainly in tropical countries. the agent has zoonotic potential, being reported in several human clinical cases. the suspected vector is the tick rhipicephalus sanguineus (sensu lato), widely distributed in animals and humans in the tropical regions of south america, africa, asia and australia, but also in southern europe. only few s ...201626984538
exposure of dogs to spotted fever group rickettsiae in urban sites associated with human rickettsioses in costa rica.the zoonotic transmission cycles of rickettsia rickettsii and other spotted fever group (sfg) rickettsiae in latin america have usually been associated with rural or sylvatic environments, although domestic dogs can be implicated in more populated settings. in this study, exposure of dogs to sfg rickettsiae in the greater metropolitan area of costa rica was investigated. dogs from sites associated with human cases and from dog shelters were evaluated by indirect immunofluorescence assay (ifa) us ...201626995323
risk factors for bartonella species infection in blood donors from southeast brazil.bacteria from the genus bartonella are emerging blood-borne bacteria, capable of causing long-lasting infection in marine and terrestrial mammals, including humans. bartonella are generally well adapted to their main host, causing persistent infection without clinical manifestation. however, these organisms may cause severe disease in natural or accidental hosts. in humans, bartonella species have been detected from sick patients presented with diverse disease manifestations, including cat scrat ...201626999057
phylogeography of rhipicephalus sanguineus sensu lato and its relationships with climatic factors.brown dog ticks morphologically identifiable as rhipicephalus sanguineus sensu lato, are distributed world-wide and their systematics is controversial. results of genetic and reproductive compatibility studies of geographically distinct populations of r. sanguineus s.l. indicate that the r. sanguineus complex is paraphyletic. to further elucidate systematic relationships within r. sanguineus s.l. and geographic boundaries of its lineages, we conducted a phylogeographical study of 136 tick specim ...201627003273
factors associated with anaplasma spp. seroprevalence among dogs in the united states.dogs in the united states are hosts to a diverse range of ticks and tick-borne pathogens, including a. phagocytophilum, an important emerging canine and human pathogen. previously, a companion animal parasite council (capc)-sponsored workshop proposed factors purported to be associated with the infection risk for tick-transmitted pathogens in dogs in the united states, including climate conditions, socioeconomic characteristics, local topography, and vector distribution.201627004557
molecular evidence of vector-borne pathogens in dogs and cats and their ectoparasites in algiers, algeria.in algeria, only limited information is currently available on the prevalence of emergent canine and feline vector-borne diseases. the aim of the present work was to detect by qpcr vector-associated bacteria in stray dogs and cats and their ectoparasites from algiers. 18/117 (15.38%) dogs and 2/107 (1.87%) cats were positive for at least one vector-borne agent. coxiella burnetii and bartonella henselae were identified in 1/117 (0.85%) dog individually. ehrlichia canis dna was detected in 17/117 ...201627012917
deltamethrin-mediated toxicity and cytomorphological changes in the midgut and nervous system of the mayfly callibaetis radiatus.immature instars of mayflies are important constituents of the food web in aquatic ecosystems (especially in neotropical regions) and they are among the most susceptible arthropods to pyrethroid insecticides. these insecticides have been recognized as important stressors of freshwater ecosystems, but their cellular effects in aquatic insects have been neglected. here, we assessed the susceptibility to deltamethrin (a typical type ii pyrethroid) as well as the deltamethrin-mediated cytomorphologi ...201627031238
tick-borne infections in human and animal population worldwide.the abundance and activity of ectoparasites and its hosts are affected by various abiotic factors, such as climate and other organisms (predators, pathogens and competitors) presenting thus multiples forms of association (obligate to facultative, permanent to intermittent and superficial to subcutaneous) developed during long co-evolving processes. ticks are ectoparasites widespread globally and its eco epidemiology are closely related to the environmental conditions. they are obligatory hematop ...201527047089
new molecular data shed light on the global phylogeny and species limits of the rhipicephalus sanguineus complex.the rhipicephalus sanguineus complex is a group of closely related tick species distributed all around the world. in this study, using mitochondrial 16s ribosomal dna, new specimens of r sanguineus sensu lato from turkey and rhipicephalus camicasi from kenya, were evaluated together with available sequences of this complex in genbank. our objectives were to delimit the complex, re-evaluate its global phylogeny and develop a reconstruction of its biogeographic history. given turkey's geographical ...201627050755
retrospective analyses of dogs found serologically positive for ehrlichia canis in cebu, philippines from 2003 to 2014.the study aimed to document the clinical and hematological observations of dogs found serologically positive for ehrlichia canis and to identify parameters or factors that are associated with the disease with focus on the anemic and thrombocytopenic state of the infected dogs.201627051183
hematobiochemical changes in ehrlichiosis in dogs of anand region, gujarat.the present research work was undertaken to study the diagnostic importance of hematobiochemical changes in naturally occurring ehrlichiosis in dogs of anand region, gujarat irrespective of their age, breed, and sex.201527065635
partial characterization of a novel anti-inflammatory protein from salivary gland extract of hyalomma anatolicum anatolicum 77acari: ixodidae) ticks.hyalomma anatolicum anatolicum ticks transmit theileria annulata, causative agent of tropical theileriosis to cattle and buffaloes causing a major economic loss in terms of production and mortality in tropical countries. ticks have evolved several immune evading strategies to circumvent hosts' rejection and achieve engorgement. successful feeding of ticks relies on a pharmacy of chemicals located in their complex salivary glands and secreted saliva. these chemicals in saliva could inhibit host i ...201527065646
efficacy of a novel oral formulation of sarolaner (simparica™) against four common tick species infesting dogs in europe.the efficacy of single oral treatment of sarolaner (simparica™, zoetis), a novel isoxazoline compound, was evaluated against four tick species known to commonly infest dogs in europe. eight laboratory studies were conducted using adult purpose-bred beagle dogs. in each study, 16 animals were randomly allocated to one of two treatment groups based on pre-treatment host-suitability tick counts. dogs were infested with 50 unfed adult dermacentor reticulatus (two studies), ixodes hexagonus (three st ...201627068640
distribution of spotted fever group rickettsiae in hard ticks (ixodida: ixodidae) from panamanian urban and rural environments (2007-2013).tick-borne rickettsiosis is an important emerging disease in panama; to date, there have been 12 confirmed cases, including eight fatalities. to evaluate the distribution of rickettsiae in panamanian ticks, we collected questing and on-host ticks in urban and rural towns in elevations varying between 0 and 2300 m. a total of 63 sites (13 urban and 50 rural towns) were used to develop models of spatial distributions. we found the following tick species: rhipicephalus sanguineus s.l. (present in 5 ...201627068930
rhipicephalus sanguineus (acari: ixodidae) biting a human being in portoalegre city, rio grande do sul, brazil.we report the finding of a female brown dog tick, rhipicephalus sanguineus (acari: ixodidae) on the scalp of a male patient inporto alegre, rio grande do sul, brazil. human parasitism by this tick is rare and has seldomly been reported in the literature, despite its recognized importance since it can act as a vector of rickettsia rickettsii, the agent of spotted fever.201627074329
design of the ataq peptide and its evaluation as an immunogen to develop a rhipicephalus vaccine.tick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. however, the current commercially available vaccines in different strains of rhipicephalus ...201627084468
quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, canis lupus familiaris.we have recently shown that repellency of the tick rhipicephalus sanguineus sensu lato by the tick resistant dog breed, the beagle, is mediated by volatile organic compounds (vocs) 2-hexanone and benzaldehyde present in beagle odour. ectoparasite location of animal hosts is affected by variation in these odour components and their ratios. the aim of this study was to quantify the release rate, and the ratio, of 2-hexanone and benzaldehyde from beagles. the odour of three beagles was collected, f ...201627085013
genetic and immunological evidences of borrelia burgdorferi in dog in thailand.lyme disease is a tick-borne zoonotic disease caused by spirochete borrelia burgdorferi. it is transmitted from animals to humans by the bite of infected ticks of the genus ixodes. although lyme disease has been reported in china and japan, the disease has never been reported in thailand. blood samples and ticks were collected from 402 dogs from 7 and 3 animal clinics in chiang mai and phuket provinces, thailand, respectively. blood samples were tested for antibodies against b. burgdorferi, anap ...201627086427
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