Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| modulation of doxorubicin-induced oxidative stress by a grape (vitis vinifera l.) seed extract in normal and tumor cells. | the major limitation of doxorubicin (dox) clinical use is the development of chronic and acute toxic side effects induced through the generation of reactive oxygen species. the present work was designated to investigate in vitro effects of a red grape-seed hydroethanolic extract burgund mare (bm), in associated administration with dox (30 min before drug administration) in normal (hfl-1) and tumor cell lines (hepg2 and mls). the bm concentrations administered were below the level of the extract ... | 2012 | 22519870 |
| alternaria sp. mg1, a resveratrol-producing fungus: isolation, identification, and optimal cultivation conditions for resveratrol production. | due to its potential in preventing or slowing the occurrence of many diseases, resveratrol (3,5,4'-trihydroxystilbene) has attracted great research interest. the objective of this study was to identify microorganisms from selected plants that produce resveratrol and to optimize the conditions for resveratrol production. endophytes from merlot wine grapes (vitis vinifera l. cv. merlot), wild vitis (vitis quinquangularis rehd.), and japanese knotweed (polygonum cuspidatum siebold & zucc.) were iso ... | 2012 | 22526800 |
| profiling of hydroxycinnamoyl tartrates and acylated anthocyanins in the skin of 34 vitis vinifera genotypes. | the diversity of berry skin flavonoids in grape genotypes has been previously widely investigated with regard to major compounds (nonacylated anthocyanins and flavonols), but much less with regard to acylated anthocyanins and hydroxycinnamoyl tartrates (hcts). in this study, the composition of the phenolic fraction of the berry skin (free and acylated anthocyanins, flavonols, and hcts) was assessed on 34 grapevine genotypes grown in a collection vineyard in northwestern italy. the phenolic fract ... | 2012 | 22533602 |
| auxin-responsive grape aux/iaa9 regulates transgenic arabidopsis plant growth. | we report the characterization of vviaa9, an auxin/indole-3-acetic acid (aux/iaa) protein, in grapevine (vitis vinifera l.). vviaa9 was expressed abundantly in leaves and berries. vviaa9 transcription was drastically upregulated from anthesis to onset of ripening (termed véraison), in which berry diameter rapidly increased. treatment with exogenous iaa induced vviaa9 expression in grape leaves, suggesting that vviaa9 is an auxin-responsive aux/iaa protein. the overexpression of vviaa9 in arabido ... | 2012 | 22535320 |
| determination of pesticide residues in wine by membrane-assisted solvent extraction and high-performance liquid chromatography-tandem mass spectrometry. | the determination of pesticides in food products is an essential issue to guarantee food safety and minimise health risks of consumers. a protocol based on membrane-assisted solvent extraction and liquid chromatography-tandem mass spectrometry (hplc-ms/ms) that allows the determination of 18 pesticides in red wine at minimum labour effort for sample preparation was developed and validated. ten millilitres of wine were extracted using 100 μl of toluene filled in a non-porous polyethylene membrane ... | 2012 | 22538775 |
| effect of yearly conditions and management practices on ochratoxin a production in sultana seedless vineyards. | sun drying of seedless grapes (vitis vinifera cv. sultanina) is widely practised in the western aegean region providing turkey with a significant share in the world trade of sultanas. research was initiated in 1998 to determine the incidence of ochratoxin a (ota), to identify the major factors resulting in contamination and to develop techniques to reduce or prevent contamination. this paper assesses ota formation in five experimental vineyards located in manisa province of turkey between 1998 a ... | 2012 | 22545668 |
| identification and characterization of the defensin-like gene family of grapevine. | defensins are a class of small and diverse cysteine-rich proteins found in plants, insects, and vertebrates, which share a common tertiary structure and usually exert broad-spectrum antimicrobial activities. we used a bioinformatic approach to scan the vitis vinifera genome and identified 79 defensin-like sequences (defl) corresponding to 46 genes and allelic variants, plus 33 pseudogenes and gene fragments. expansion and diversification of grapevine defl has occurred after the split from the la ... | 2012 | 22550957 |
| expression of flavonoid genes in the red grape berry of 'alicante bouschet' varies with the histological distribution of anthocyanins and their chemical composition. | the mature berry of vitis vinifera 'alicante bouschet' is entirely red, but anthocyanin metabolism discloses elements of histological discontinuity. this provides an experimental system amenable to studies of compartmentalised secondary metabolism in a fleshly fruit. we compared microscopy of fixed berry sections and chemical composition of anthocyanin extracts with the expression of 41 flavonoid genes in three berry tissues. in the pericarp, anthocyanins formed membrane-encased spherical coales ... | 2012 | 22552639 |
| modelling leaf photosynthetic and transpiration temperature-dependent responses in vitis vinifera cv. semillon grapevines growing in hot, irrigated vineyard conditions. | grapevines growing in australia are often exposed to very high temperatures and the question of how the gas exchange processes adjust to these conditions is not well understood. the aim was to develop a model of photosynthesis and transpiration in relation to temperature to quantify the impact of the growing conditions on vine performance. | 2012 | 22567220 |
| suppressive subtractive hybridization method analysis and its application to salt stress in grapevine (vitis vinifera l.). | two subtracted cdna libraries of grapevines (vitis vinifera. l) under short term salt stress incubation were constructed using the suppression subtractive hybridization (ssh) method combined with the differential screening approach. the mrna isolated from leaves of the salt-tolerant grapevine cultivar razegui grown without stress was used as a "driver," and the corresponding mrnas isolated after a short-term treatment 6 or 24h of salt stress were used as "testers." the differentially expressed c ... | 2012 | 22567999 |
| design and optimization of a semicontinuous hot-cold extraction of polyphenols from grape pomace. | grape pomace contains appreciable amounts of polyphenolic compounds such as anthocyanins and procyanidins which can be recovered for use as food supplements. the extraction of these polyphenols from the pomace is usually accomplished at slightly elevated temperatures, frequently employing hydroethanolic solvents. due to governmental regulations and the cost involved in using ethanol as a solvent, as well as the loss in polyphenolics due to thermal degradation, improved extraction techniques are ... | 2012 | 22578157 |
| berry morphology and composition in irrigated and non-irrigated grapevine (vitis vinifera l.). | the present study was carried out in a 5-year-old vineyard (vitis vinifera l., cv. aglianico) located in southern italy. half of the plants (irr) were fully irrigated, whereas the other half were not irrigated (nirr). in both of the treatments, plant water status, gas exchange, photosynthetic efficiency and productive performance were determined. the arid conditions resulted in significant decreases in stem water potential in nirr (minimum values of -1.34 and -1.52 mpa in irr and nirr, respectiv ... | 2012 | 22583647 |
| supercritical extraction from vinification residues: fatty acids, α-tocopherol, and phenolic compounds in the oil seeds from different varieties of grape. | supercritical fluid extraction has been widely employed in the extraction of high purity substances. in this study, we used the technology to obtain oil from seeds from a variety of grapes, from vinification residues generated in the southern region of the state of rio grande do sul, brazil. this work encompasses three varieties of vitis vinifera (moscato giallo, merlot, and cabernet sauvignon) and two of vitis labrusca (bordô e isabel), harvested in 2005 and 2006. we obtained the highest oil co ... | 2012 | 22593706 |
| cloning and molecular characterization of a putative bzip transcription factor vvbzip23 from vitis vinifera. | the proteins harboring bzip domains comprise a large family and play key roles in many cellular processes, one of them being tolerance to biotic and abiotic stresses in plants. in the present study, we characterize a putative bzip transcription factor from vitis vinifera namely vvbzip23. our studies revealed that a gfp fusion of vvbzip23 is localized in the nucleus showing vvbzip23 codes for a nuclear localized protein. vvbzip23 identified by in silico approaches from grapevine dna databases ava ... | 2013 | 22610648 |
| effects of severity of post-flowering leaf removal on berry growth and composition of three red vitis vinifera l. cultivars grown under semiarid conditions. | the effects of the severity of post-flowering leaf removal on the growth and phenolic composition of berry skin and seeds were studied in three vitis vinifera l. genotypes over two consecutive seasons, 2007 and 2008. the study was conducted in a commercial vertical shoot positioned (vsp)-trained nonirrigated vineyard of northern greece, planted with cultivars merlot, cabernet sauvignon, and sangiovese. three different severities of leaf removal in the fruit zone were applied manually at berry se ... | 2012 | 22630367 |
| comparison of a retrotransposon-based marker with microsatellite markers for discriminating accessions of vitis vinifera. | identification and knowledge concerning genetic diversity are fundamental for efficient management and use of grapevine germplasm. recently, new types of molecular markers have been developed, such as retrotransposon-based markers. because of their multilocus pattern, retrotransposon-based markers might be able to differentiate grapevine accessions with just one pair of primers. in order to evaluate the efficiency of this type of marker, we compared retrotransposon marker tvv1 with seven microsa ... | 2012 | 22653601 |
| molecular cloning, sequence characterization of a novel pepper gene nadp-icdh and its effect on cytoplasmic male sterility. | nadp-dependent isocitrate dehydrogenase (nadp-icdh) is an important enzyme involved in energy metabolism. the complete coding sequence of the pepper (capsicum annuum) nadp-icdh gene was amplified using a reverse transcriptase pcr based on the conserved sequence information of the tomato and other solanaceae plants and known highly homologous pepper ests. nucleotide sequence analysis revealed that the pepper nadp-icdh gene encodes a protein of 415 amino acids that has high homology with the prote ... | 2012 | 22653649 |
| haemostatic effect of ankaferd blood stopper(®) seen during adenoidectomy. | in turkey, ankaferd blood stopper(®) (abs) has been approved for the management of external haemorrhages and bleedings occurring during dental surgeries (goker et al., 2008). ankaferd comprises a standardized mixture of plants, including thymus vulgaris, glycyrrhiza glabra, vitis vinifera, alpinia officinarum, and urtica diodica. this study aimed to evaluate the efficacy of ab s tam ponade in the control of intra-operative bleeding occurring during ad enoidectomy performed in children under the ... | 2011 | 22654224 |
| an integrated approach to demonstrating the anr pathway of proanthocyanidin biosynthesis in plants. | proanthocyanidins (pas) are oligomers or polymers of plant flavan-3-ols and are important to plant adaptation in extreme environmental conditions. the characterization of anthocyanidin reductase (anr) and leucoanthocyanidin reductase (lar) has demonstrated the different biogenesis of four stereo-configurations of flavan-3-ols. it is important to understand whether anr and the anr pathway widely occur in the plant kingdom. here, we report an integrated approach to demonstrate the anr pathway in p ... | 2012 | 22678031 |
| rapid monitoring of grapevine reserves using atr-ft-ir and chemometrics. | predictions of grapevine yield and the management of sugar accumulation and secondary metabolite production during berry ripening may be improved by monitoring nitrogen and starch reserves in the perennial parts of the vine. the standard method for determining nitrogen concentration in plant tissue is by combustion analysis, while enzymatic hydrolysis followed by glucose quantification is commonly used for starch. attenuated total reflectance fourier transform infrared spectroscopy (atr-ft-ir) c ... | 2012 | 22688030 |
| influence of the physiological stage and the content of soluble solids on the anthocyanin extractability of vitis vinifera l. cv. tempranillo grapes. | the influence and the extent of the anthocyanin extractability from grape skins at different physiological stages and different soluble solids contents within each stage were investigated. for this purpose three different physiological stages (pre-harvest, harvest and over-ripening) and three soluble solids contents (22, 24 and 26°brix) within each stage were taken into account using vitis vinifera l. cv. tempranillo. this study revealed the influence of physiological stages and soluble solids c ... | 2012 | 22688031 |
| influence of climatic conditions on the phenolic composition of vitis vinifera l. cv. graciano. | the influence of different climatic conditions on the phenolic composition of grape skins and seeds of vitis vinifera l. cv. graciano - an autochthonous cultivar from rioja and navarra regions (spain) - was evaluated during ripening in a separate way. graciano grapes from two different vineyards with different climatic conditions and from two different vintages (2008 and 2009) were analysed. clear differences between phenolic maturity pattern of grape skins and seeds were observed. in this conte ... | 2012 | 22688036 |
| sensory representation of typicality of cabernet franc wines related to phenolic composition: impact of ripening stage and maceration time. | phenolics are responsible for important sensory properties of red wines, including colour, astringency, and possibly bitterness. from a technical viewpoint, the harvest date and the maceration duration are critical decisions for producing red wine with a distinctive style. but little is known about the evolution of phenolics and of their extractability during ripening to predict the composition of the wine and related sensory properties. the aim of this study was to understand the relationship b ... | 2012 | 22688039 |
| anthocyanin identification and composition of wild vitis spp. accessions by using lc-ms and lc-nmr. | the composition and concentration of anthocyanins of grape berry skins were analyzed in order to assess phenotypic variation between four grape wine varieties belonging to 4 different species: vitis vinifera, vitis amurensis, vitis cinerea and vitis x champinii. high-performance liquid chromatography coupled to mass spectrometry (lc-ms) and nmr spectroscopy (lc-nmr) were used to separate and identify the structure of anthocyanins present in these species. combination of lc-ms and lc-nmr data res ... | 2012 | 22688046 |
| a framework genetic map of muscadinia rotundifolia. | this study presents a framework linkage map based on microsatellite markers for muscadinia rotundifolia (1n = 20). the mapping population consisted of 206 progeny generated from a cross of two m. rotundifolia varieties, 'fry' and 'trayshed'. a total of 884 primers were tested for their ability to amplify markers: 686 amplified and 312 simple sequence repeat (ssr) primer pairs generated 322 polymorphic markers for either one or both parents. the map for the female parent 'fry' consisted of 212 ma ... | 2012 | 22688272 |
| the antioxidant effects of a polyphenol-rich grape pomace extract in vitro do not correspond in vivo using exercise as an oxidant stimulus. | fruits, such as grapes, are essential food of the mediterranean diet. grape extracts have potent antioxidant and chemopreventive properties in vitro. numerous studies have examined the effects of plant extract administration on redox status at rest in animals and humans but their results are controversial. however, there are no studies comparing the in vitro and in vivo effects of plant extracts on oxidative stress using exercise as an oxidant stimulus. thus, the aim of this study was to investi ... | 2012 | 22693650 |
| subcellular mn compartation, anatomic and biochemical changes of two grape varieties in response to excess manganese. | to explore the underlying mechanism for the high tolerance to excess manganese stress in the grape species (vitis vinifera linn), we observed the subcellular compartment of mn element, anatomic and biochemical responses of two grape cultivars (combier and shuijin) under excess mn stress in semi-controlled environmental condition. grape species exhibited typical detoxifying or tolerant mechanism as following: first, majority of mn element accumulated in leaf was excluded into cell wall or compart ... | 2012 | 22694775 |
| thiamine induced resistance to plasmopara viticola in grapevine and elicited host-defense responses, including hr like-cell death. | recently, thiamine (vitaminb1) has been shown to induce resistance against pseudomonas syringae in arabidopsis plants through priming of defense responses. in this paper, we have demonstrated the efficiency of thiamine to induce resistance against downy mildew caused by the oomycete plasmopara viticola in a susceptible vitis vinifera cultivar "chardonnay" under glasshouse controlled conditions by providing a dual mode of action involving direct antifungal activity and elicitation of host-defense ... | 2012 | 22698755 |
| effect of climate change on infection of grapevine by downy and powdery mildew under controlled environment. | plant responses to elevated co2 and temperature have been much studied in recent years, but effects of climate change on pathological responses are largerly unknown. the pathosystems grapevine (vitis vinifera) - downy mildew (plasmopara viticola) and powdery mildew (erysiphe necatrix) were chosen as models to assess the potential impact of increased co2 and temperature on disease incidence and severity under controlled environment. grapevine potted plants were grown in phytotrons under 4 differe ... | 2011 | 22702176 |
| the genes and enzymes of the carotenoid metabolic pathway in vitis vinifera l. | carotenoids are a heterogeneous group of plant isoprenoids primarily involved in photosynthesis. in plants the cleavage of carotenoids leads to the formation of the phytohormones abscisic acid and strigolactone, and c13-norisoprenoids involved in the characteristic flavour and aroma compounds in flowers and fruits and are of specific importance in the varietal character of grapes and wine. this work extends the previous reports of carotenoid gene expression and photosynthetic pigment analysis by ... | 2012 | 22702718 |
| impact of must sugar reduction by membrane applications on volatile composition of verdejo wines. | climate changes are inducing increased sugar levels of must, which produces negative effects on wine quality, as unbalanced wines with high degrees of alcohol. so, effective strategies to control the increase of sugar levels in must have been studied. one of them is the use of a membrane process, and this is applied in this work. the sugar level of white must from verdejo (vitis vinifera variety) was reduced using diverse membrane processes, and the effect of this fact on the volatile compositio ... | 2012 | 22703609 |
| feasibility of incorporating treated lignins in fiberboards made from agricultural waste. | this paper studies the feasibility of incorporating treated lignins in fiberboards made from vitis vinifera as an agricultural waste. the treated lignins are the purified kraft lignin and the alkaline hydrolyzed kraft lignin. v. vinifera raw material and its fibers were characterized in terms of chemical composition and the results were compared to other biomass species. the chemical composition of treated lignins shows that they have high purity compared to the lignin raw material. the lignin-v ... | 2012 | 22704807 |
| identification of new flavan-3-ol monoglycosides by uhplc-esi-q-tof in grapes and wine. | flavan-3-ol monoglycosides, having four aglycons (+)-catechin, (-)-epicatechin, (-)-epigallocatechin and epicatechin gallate monomeric units, are detected for the first time in vitis vinifera l. cv. merlot grape seeds and wine. these compounds were analyzed in red wine, seed and skin extracts by electrospray ionization quadrupole time of flight mass spectrometry (ms) in negative mode. fragment ions derived from retro-diels alder, heterocyclic ring fragmentation, benzofuran forming fragmentation ... | 2012 | 22707165 |
| effects of bois noir on carbon assimilation, transpiration, stomatal conductance of leaves and yield of grapevine (vitis vinifera) cv. chardonnay. | bois noir (bn) is one of the main phytoplasma diseases of grapevine (vitis vinifera). it is widespread, and can cause severe losses in european vineyards. the infective agent colonizes phloem elements and induces visible symptoms of leaf yellowing or reddening after a relatively long incubation period. as the most sensitive cultivars to bn, chardonnay plants were grouped as healthy or symptomatic in spring, based on the records from the previous year. leaf gas exchange and chlorophyll a fluoresc ... | 2012 | 22715513 |
| discovery of novel rules for g-quadruplex-forming sequences in plants by using bioinformatics methods. | the g-quadruplex is one of the most frequently studied secondary dna structures and consists of 4 guanine residues that interact through watson-crick and hoogsteen pairing. the g-quadruplex formation is thought to be a molecular switch for gene expression. genome-wide analyses of g-quadruplexes have been published for many species; however, only one genome-wide analysis of g-quadruplexes in plants has been reported. here, we propose a new approach involving a two-step procedure for identifying g ... | 2012 | 22721688 |
| rapid measurement of methyl cellulose precipitable tannins using ultraviolet spectroscopy with chemometrics: application to red wine and inter-laboratory calibration transfer. | information relating to tannin concentration in grapes and wine is not currently available simply and rapidly enough to inform decision-making by grape growers, winemakers, and wine researchers. spectroscopy and chemometrics have been implemented for the analysis of critical grape and wine parameters and offer a possible solution for rapid tannin analysis. we report here the development and validation of an ultraviolet (uv) spectral calibration for the prediction of tannin concentration in red w ... | 2012 | 22732536 |
| protective effects of hv-p411 complex against d-galactosamine-induced hepatotoxicity in rats. | this study examined the hepatoprotective effect of the hv-p411 complex, an herbal extract mixture from the seeds of vitis vinifera, schisandra chinensis and taraxacum officinale, against d-galactosamine (d-galn)-induced hepatitis. hepatotoxicity was induced by d-galn (700 mg/kg, i.p.), and the hv-p411 complex was administered orally 48, 24, and 2 h before and 6 h after d-galn injection. increases in serum aminotransferase activity and lipid peroxidation and a decrease in hepatic glutathione cont ... | 2012 | 22745064 |
| solid phase microextraction as a reliable alternative to conventional extraction techniques to evaluate the pattern of hydrolytically released components in vitis vinifera l. grapes. | in present research, headspace solid-phase microextraction (hs-spme) followed by gas chromatography-mass spectrometry (gc-qms), was evaluated as a reliable and improved alternative to the commonly used liquid-liquid extraction (lle) technique for the establishment of the pattern of hydrolytically released components of 7 vitis vinifera l. grape varieties, commonly used to produce the world-famous madeira wine. since there is no data available on their glycosidic fractions, at a first step, two h ... | 2012 | 22748548 |
| linkage disequilibrium and spatial aggregation of genotypes in sexually reproducing populations of erysiphe necator. | random mating and recombination in heterothallic ascomycetes should result in high genotypic diversity, 1:1 mating-type ratios, and random associations of alleles, or linkage equilibrium, at different loci. to test for random mating in populations of the grape powdery mildew fungus erysiphe necator, we sampled isolates from vineyards of vitis vinifera in burdett, ny (ny09) and winchester, va (va09) at the end of the epidemic in fall 2009. we also sampled isolates from the same winchester, va vin ... | 2012 | 22755546 |
| crystal, solution and in silico structural studies of dihydrodipicolinate synthase from the common grapevine. | dihydrodipicolinate synthase (dhdps) catalyzes the rate limiting step in lysine biosynthesis in bacteria and plants. the structure of dhdps has been determined from several bacterial species and shown in most cases to form a homotetramer or dimer of dimers. however, only one plant dhdps structure has been determined to date from the wild tobacco species, nicotiana sylvestris (blickling et al. (1997) j. mol. biol. 274, 608-621). whilst n. sylvestris dhdps also forms a homotetramer, the plant enzy ... | 2012 | 22761676 |
| carotenoids, total polyphenols and antioxidant activity of grapes (vitis vinifera) cultivated in organic and conventional systems. | organic agriculture involve plants which are cultivated without using synthetic pesticides, herbicides or fertilizers and promotes biodiversity, biological cycles and improve the product quality. the carotenoids, total polyphenols and the antioxidant activity from skins of some wine and table grapes cultivated in organic and conventional agriculture were studied. | 2012 | 22762349 |
| berry flesh and skin ripening features in vitis vinifera as assessed by transcriptional profiling. | ripening of fleshy fruit is a complex developmental process involving the differentiation of tissues with separate functions. during grapevine berry ripening important processes contributing to table and wine grape quality take place, some of them flesh- or skin-specific. in this study, transcriptional profiles throughout flesh and skin ripening were followed during two different seasons in a table grape cultivar 'muscat hamburg' to determine tissue-specific as well as common developmental progr ... | 2012 | 22768087 |
| a core-shell column approach to a comprehensive high-performance liquid chromatography phenolic analysis of vitis vinifera l. and interspecific hybrid grape juices, wines, and other matrices following either solid phase extraction or direct injection. | four high-throughput reverse-phase chromatographic protocols utilizing two different core-shell column chemistries have been developed to analyze the phenolic profiles of complex matrices, specifically targeting juices and wines produced from interspecific hybrid grape cultivars. following pre-fractionation via solid-phase extraction or direct injection, individual protocols were designed to resolve, identify and quantify specific chemical classes of compounds including non-anthocyanin monomeric ... | 2012 | 22771069 |
| modulation of protein phosphorylation, n-glycosylation and lys-acetylation in grape (vitis vinifera) mesocarp and exocarp owing to lobesia botrana infection. | grapevine (vitis vinifera) is an economically important fruit crop that is subject to many types of insect and pathogen attack. to better elucidate the plant response to lobesia botrana pathogen infection, we initiated a global comparative proteomic study monitoring steady-state protein expression as well as changes in n-glycosylation, phosphorylation, and lys-acetylation in control and infected mesocarp and exocarp from v. vinifera cv italia. a multi-parallel, large-scale proteomic approach emp ... | 2012 | 22778145 |
| defence signalling triggered by flg22 and harpin is integrated into a different stilbene output in vitis cells. | plants can activate defence to pathogen attack by two layers of innate immunity: basal immunity triggered by pathogen-associated molecular pattern (pamp) triggered immunity (pti) and effector-triggered immunity (eti) linked with programmed cell death. flg22 and harpin are evolutionary distinct bacterial pamps. we have previously shown that harpin triggers hypersensitive cell death mimicking eti in vitis rupestris, but not in the vitis vinifera cultivar 'pinot noir'. in contrast, the bacterial pa ... | 2012 | 22792328 |
| mapping of crown gall resistance locus rcg1 in grapevine. | agrobacteria are efficient plant pathogens. they are able to transform plant cells genetically resulting in abnormal cell proliferation. cultivars of vitis vinifera are highly susceptible to many virulent agrobacterium strains but certain wild vitis species, including vitis amurensis have resistant genotypes. studies of the molecular background of such natural resistance are of special importance, not only for practical benefits in agricultural practice but also for understanding the role of pla ... | 2012 | 22801874 |
| chemometric analysis for the detection of biogenic amines in chilean cabernet sauvignon wines: a comparative study between organic and nonorganic production. | in this work, the presence of biogenic amines (bas) was correlated with the type of wine grape culture (traditional or organic) and their concentration in the different stages of winemaking (must, alcoholic fermentation [af] and malolactic fermentation [mlf]). the formation of ba occurred mainly during mlf in which the percentages for putrescine, cadaverine, phenylethylamine, histamine, and tyramine were 100%, 70%, 13%, 61%, and 44% for the wines produced with traditional grapes and 100%, 94%, 2 ... | 2012 | 22809090 |
| grape berry bacterial microbiota: impact of the ripening process and the farming system. | wine grapes are a primary source of microbial communities that play a prominent role in the quality of grapes prior to harvesting, as well as in the winemaking process. this study investigated the dynamics and diversity of the epiphytic bacteria on the grape berry surface during maturation. the quantitative and qualitative effects of conventional and organic farming systems on this microbial community were investigated, using both cultivation-dependent and independent approaches. analyses of gra ... | 2012 | 22809638 |
| characterization of a putative grapevine zn transporter, vvzip3, suggests its involvement in early reproductive development in vitis vinifera l. | zinc (zn) deficiency is one of the most widespread mineral nutritional problems that affect normal development in plants. because zn cannot passively diffuse across cell membranes, it must be transported into intracellular compartments for all biological processes where zn is required. several members of the zinc-regulated transporters, iron-regulated transporter-like protein (zip) gene family have been characterized in plants, and have shown to be involved in metal uptake and transport. this st ... | 2012 | 22824090 |
| biosynthesis of wine aroma: transcript profiles of hydroxymethylbutenyl diphosphate reductase, geranyl diphosphate synthase, and linalool/nerolidol synthase parallel monoterpenol glycoside accumulation in gewürztraminer grapes. | in developing grapevine (vitis vinifera l.) berries, precursor volatile organic compounds (pvocs) are largely stored as glycosides which may be hydrolyzed to release vocs during fruit ripening, wine making, or aging. vocs can be further transformed by yeast metabolism. together, these processes contribute to complexity of wine aromas. floral and citrus odors of many white wine varietals are attributed to monoterpenes and monoterpene alcohols, while phenolic compounds, norisoprenoids, and other v ... | 2012 | 22824963 |
| high-throughput sequence analysis of small rnas in grapevine (vitis vinifera l.) affected by grapevine leafroll disease. | grapevine leafroll disease (glrd) is one of the most economically important virus diseases of grapevine (vitis spp.) worldwide. in this study, we used high-throughput sequencing of cdna libraries made from small rnas (srnas) to compare profiles of srna populations recovered from own-rooted merlot grapevines with and without glrd symptoms. the data revealed the presence of srnas specific to grapevine leafroll-associated virus 3, hop stunt viroid (hpsvd), grapevine yellow speckle viroid 1 (gysvd-1 ... | 2012 | 22827483 |
| comparison on phenolic compounds and antioxidant properties of cabernet sauvignon and merlot wines from four wine grape-growing regions in china. | the antioxidant activities in the cabernet sauvignon and merlot wines from four wine grape-growing regions in china were measured by different analytical assays: 2,2-diphenyl-1-picrylhydrazyl (dpph·), cupric reducing antioxidant capacity (cuprac), superoxide radical-scavenging activity (srsa) and the contents of total phenols, total flavonoids, total flavanols and total anthocyanins were determined. the results showed that the contents of phenolic compounds and the levels of antioxidant activity ... | 2012 | 22832882 |
| phenotypic and histochemical traits of the interaction between plasmopara viticola and resistant or susceptible grapevine varieties. | grapevine downy mildew, caused by plasmopara viticola, is a very serious disease affecting mainly vitis vinifera cultivated varieties around the world. breeding for resistance through the crossing with less susceptible species is one of the possible means to reduce the disease incidence and the application of fungicides. the hybrid bianca and some of its siblings are considered very promising but their resistance level can vary depending on the pathogen strain. moreover, virulent strains charact ... | 2012 | 22852828 |
| tissue-specific and developmental modifications of grape cell walls influence the adsorption of proanthocyanidins. | cell wall material from vitis vinifera l. cv. cabernet sauvignon grape skin and flesh was isolated at different stages of grape maturity to determine whether developmental changes in cell wall composition in different tissue types influence the binding of proanthocyanidins (pas). trends in cell wall adsorption of, and selectivity for, pas were determined using two skin pas that differed in their average molecular masses. flesh cell walls consistently bound a higher amount of pa than those from s ... | 2012 | 22860923 |
| identification of genes differentially expressed in grapevine associated with resistance to elsinoe ampelina through suppressive subtraction hybridization. | anthracnose, caused by the biotrophic fungus elsinoe ampelina, is an economically devastating disease of grapevine (vitis vinifera l.) prevalent in warm and humid regions of the world. in order to investigate the molecular resistance mechanisms and identify genes related to anthracnose resistance in grapevine, a suppression subtractive hybridization (ssh) library was constructed using mixed cdnas prepared from leaves of chinese wild vitis quinquangularis clone 'shang-24', cdna prepared from leav ... | 2012 | 22864229 |
| a reference genetic map of muscadinia rotundifolia and identification of ren5, a new major locus for resistance to grapevine powdery mildew. | muscadinia rotundifolia, a species closely related to cultivated grapevine vitis vinifera, is a major source of resistance to grapevine downy and powdery mildew, two major threats to cultivated traditional cultivars of v. vinifera respectively caused by the oomycete plasmopara viticola and the ascomycete erisyphe necator. the aim of the present work was to develop a reference genetic linkage map based on simple sequence repeat (ssr) markers for m. rotundifolia. this map was created using s1 m. r ... | 2012 | 22865124 |
| association of rga-sscp markers with resistance to downy mildew and anthracnose in grapevines. | downy mildew (plasmopara viticola) and anthracnose (sphaceloma ampelinum) are two major diseases that severely affect most grapevine (vitis vinifera) cultivars grown commercially in thailand. progress of conventional breeding programs of grapevine for improved resistance to these diseases can be speeded up by selection of molecular markers associated with resistance traits. we evaluated the association between 13 resistance gene analog (rga)-single-strand conformation polymorphism (sscp) markers ... | 2012 | 22869536 |
| the mode of host resistance to plasmopara viticola infection of grapevines. | abstract the resistance and susceptibility of grapevines to downy mildew (dm) disease caused by plasmopara viticola were compared among different cultivars/accessions belonging to vitis vinifera, v. rotundifolia, and 10 oriental vitis species. after inoculation with p. viticola pathogen, no symptom was found in v. rotundifolia grapevines at all, while oriental species v. davidii and v. piasezkii, like v. vinifera, were susceptible to dm disease. the other eight oriental vitis species showed vari ... | 2012 | 22877313 |
| identification of the dehydrin gene family from grapevine species and analysis of their responsiveness to various forms of abiotic and biotic stress. | dehydrins (dhns) protect plant cells from desiccation damage during environmental stress, and also participate in host resistance to various pathogens. in this study, we aimed to identify and characterize the dhn gene families from vitis vinifera and wild v. yeshanensis, which is tolerant to both drought and cold, and moderately resistant to powdery mildew. | 2012 | 22882870 |
| molecular characterization of vvsdir1 from vitis vinifera and its functional analysis by heterologous expression in nicotiana tabacum. | the proteins harboring really interesting new gene (ring) finger domains comprise a large family and play key roles in a variety of cellular processes. one among them is the tolerance to biotic and abiotic stresses in plants. in the present study, we characterize vitis vinifera salt- and drought-induced ring finger 1 (vvsdir1) a homologue of the arabidopsis sdir1 gene obtained from v. vinifera. the vvsdir1 gene was identified using in silico approaches and encodes a membrane-localized protein. t ... | 2013 | 22886312 |
| effect of different drying methods and storage time on the retention of bioactive compounds and antibacterial activity of wine grape pomace (pinot noir and merlot). | the effects of different drying methods (40 °c conventional and vacuum oven, 25 °c ambient air and freeze dry) on the stability of two red wine grape (pinot noir, pn and merlot, m) byproducts, pomace containing skins and seeds (p) and pomace containing skins only (s) were investigated. freeze dried samples retained the highest bioactive compounds with total phenolic content (tpc) of 21.19-67.74 mg gae/g d.m., anthocyanin content (acy) of 0.35-0.76 mg mal-3-glu/g d.m., dpph antiradical scavenge a ... | 2012 | 22908851 |
| construction of a high-density genetic map for grape using next generation restriction-site associated dna sequencing. | genetic mapping and qtl detection are powerful methodologies in plant improvement and breeding. construction of a high-density and high-quality genetic map would be of great benefit in the production of superior grapes to meet human demand. high throughput and low cost of the recently developed next generation sequencing (ngs) technology have resulted in its wide application in genome research. sequencing restriction-site associated dna (rad) might be an efficient strategy to simplify genotyping ... | 2012 | 22908993 |
| comparison between modified dna extraction protocols and commercial isolation kits in grapevine (vitis vinifera l.). | various protocols have been developed and used for dna extraction in grapevine. however, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. in our study, the dna yield and purity obtained using six methods--namely three dna isolation protocols and three commercial dna isolation kits--were compared. modifications were made and the isolation steps were shortened in the previously develop ... | 2012 | 22911604 |
| identification of trans-acting sirnas and their regulatory cascades in grapevine. | trans-acting small interfering rnas (ta-sirnas) play an essential role in the regulation of plant gene expression, but relevant reports are still limited. bioinformatic analyses indicate that many ta-sirna-producing loci (tass) are present in plants, implying the existence of as yet undiscovered ta-sirnas and related regulatory pathways. to expand our knowledge of these plant gene regulators, we applied high-throughput computational and experimental methods to grapevine (vitis vinifera l.). | 2012 | 22914222 |
| antioxidant profile and in vitro cardiac radical-scavenging versus pro-oxidant effects of commercial red grape juices ( vitis vinifera l. cv. aglianico n.). | several works have reported on the pharmaceutical usefulness of grape phytochemicals. nevertheless, the scientific literature needs further studies to consider grape extracts as useful dietary supplements. the aim of the present work was to hypothesize for the first time the use of whole commercial red grape juices as food supplements potentially useful against both physiological and induced cardiac oxidative stress. first of all, the results indicated a good antioxidant stability of the juice s ... | 2012 | 22917058 |
| chloroplast genome diversity in portuguese grapevine (vitis vinifera l.) cultivars. | grapevine chloroplast (cp) dna diversity was analysed for the first time through amplification and digestion of fragments of the large single copy (lsc) region by polymerase chain reaction-restriction fragment length polymorphism methodology and also by amplification of three microsatellite loci, previously described as polymorphic in grapevine. thirty-eight grapevine cultivars collected mainly in the north of portugal, including some neglected cultivars, four international cultivars (chasselas, ... | 2013 | 22918875 |
| potential anticancer properties of grape antioxidants. | dietary intake of foods rich in antioxidant properties is suggested to be cancer protective. foods rich in antioxidant properties include grape (vitis vinifera), one of the world's largest fruit crops and most commonly consumed fruits in the world. the composition and cancer-protective effects of major phenolic antioxidants in grape skin and seed extracts are discussed in this review. grape skin and seed extracts exert strong free radical scavenging and chelating activities and inhibit lipid oxi ... | 2012 | 22919383 |
| environmental factors affect the activity of biocontrol agents against ochratoxigenic aspergillus carbonarius on wine grape. | the influence of temperature and relative humidity (rh) on the activity of three biocontrol agents-the yeast metschnikowia pulcherrima ls16 and two strains of the yeast-like fungus aureobasidium pullulans ls30 and au34-2-against infection by a. carbonarius and ochratoxin a (ota) accumulation in wine grape berries was investigated in lab-scale experiments. the presence of wounds on grape skin dramatically favored infection of berries by a. carbonarius strain a1102, since unwounded berries showed ... | 2012 | 22921968 |
| the grapevine root-specific aquaporin vvpip2;4n controls root hydraulic conductance and leaf gas exchange under well-watered conditions but not under water stress. | we functionally characterized the grape (vitis vinifera) vvpip2;4n (for plasma membrane intrinsic protein) aquaporin gene. expression of vvpip2;4n in xenopus laevis oocytes increased their swelling rate 54-fold. northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that vvpip2;4n is the most expressed pip2 gene in root. in situ hybridization confirmed root localization in the cortical parenchyma and close to the endodermis. we then constitutively overexpr ... | 2012 | 22923680 |
| influence of intensity and duration of regulated deficit irrigation on erythroneura elegantula (hemiptera: cicadellidae) on grape. | we conducted studies in a paso robles, ca, grape (vitis vinifera l.) vineyard in 2002 and 2003 to estimate the impact of regulated deficit irrigation (rdi) intensity and duration on western grape leafhopper, erythroneura elegantula osborn. treatments were based on deficit intensity, 50 and 25% of standard irrigation (moderate and severe deficits, respectively), and deficit duration, 3 or 6 wk time, initiated at the grape phenological stage of berry set. the standard irrigation served as the cont ... | 2012 | 22928309 |
| regulation of the grapevine polygalacturonase-inhibiting protein encoding gene: expression pattern, induction profile and promoter analysis. | regulation of defense in plants is a complex process mediated by various signaling pathways. promoter analysis of defense-related genes is useful to understand these signaling pathways involved in regulation. to this end, the regulation of the polygalacturonase-inhibiting protein encoding gene from vitis vinifera l. (vvpgip1) was analyzed with regard to expression pattern and induction profile as well as the promoter in terms of putative regulatory elements present, core promoter size and the st ... | 2013 | 22932820 |
| genome sequence of novosphingobium sp. strain rr 2-17, a nopaline crown gall-associated bacterium isolated from vitis vinifera l. grapevine. | novosphingobium sp. strain rr 2-17 is an n-acyl homoserine lactone (ahl)-producing bacterium isolated from the crown gall tumor of a grapevine. to our knowledge, this is the first draft genome announcement of a plant-associated strain from the genus novosphingobium. | 2012 | 22933764 |
| genome sequence of methylobacterium sp. strain gxf4, a xylem-associated bacterium isolated from vitis vinifera l. grapevine. | methylobacterium sp. strain gxf4 is an isolate from grapevine. here we present the sequence, assembly, and annotation of its genome, which may shed light on its role as a grapevine xylem inhabitant. to our knowledge, this is the first genome announcement of a plant xylem-associated strain of the genus methylobacterium. | 2012 | 22933776 |
| attachment of chloride anion to sugars: mechanistic investigation and discovery of a new dopant for efficient sugar ionization/detection in mass spectrometers. | a new method for efficient ionization of sugars in the negative-ion mode of electrospray mass spectrometry is presented. instead of using strongly hydrophobic dopants such as dichloromethane or chloroform, efficient ionization of sugars has been achieved by using aqueous hcl solution for the first time. this methodology makes it possible to use hydrophilic dopants, which are more appropriate for chromatographic separation techniques with efficient sugar ionization and detection in mass spectrome ... | 2012 | 22936644 |
| expression and in situ localization of two major pr proteins of grapevine berries during development and after uv-c exposition. | in grapevine vitis vinifera l. cv pinot noir, the pathogenesis-related (pr) proteins chi4d and tl3 are among the most abundant extractable pr proteins of ripe berries and accumulate during berry ripening from véraison until full maturation. evidence was supplied in favor of the involvement of these two protein families in plant defense mechanisms and plant development. in order to better understand chi4d and tl3 function in grapevine, we analyzed their temporal and spatial pattern of expression ... | 2012 | 22937077 |
| [cadmium accumulation, subcellular distribution, and chemical forms in vitis vinifera cv. chardonnay grapevine]. | a pot culture experiment was conducted to study the cd absorption, cd subcellular distribution, and cd chemical forms in one-year old self-rooted chardonnay and so4 rootstock-grafted chardonnay grapevine after root-irrigating with different concentration cdcl and cacl2. in the grapevine, the absorbed cd was mostly distributed in underground organs (root and rhizome). in treatment 4 mmol x l(-1) of cdcl2, 77.1% and 1.4% of the absorbed cd in self-rooted chardonnay were accumulated in underground ... | 2012 | 22937650 |
| the influence of fining agents on the removal of some pesticides from white wine of vitis vinifera l. cv. emir. | the influences of fining agents (activated carbon, casein, kieselsol-gelatine, bentonite and polyvinylpolypyrrolidone) and their doses (low, middle, high) on the removal of six pesticides used in viticulture (vinclozolin, penconazole, α-endosulfan, imazalil, nuarimol and tetradifon) from white wine were investigated. the pesticides were added into white wine obtained from the emir grape, and then the wine was clarified with the use of fining agents at low, middle and high doses. after the fining ... | 2012 | 22939932 |
| metabolomics reveals simultaneous influences of plant defence system and fungal growth in botrytis cinerea-infected vitis vinifera cv. chardonnay berries. | botrytis cinerea is a fungal plant pathogen of grape berries, leading to economic and quality losses in wine production. the global metabolite changes induced by b. cinerea infection in grape have not been established to date, even though b. cinerea infection is known to cause significant changes in chemicals or metabolites. in order to better understand metabolic mechanisms linked to the infection process and to identify the metabolites associated with b. cinerea infection, (1)h nmr spectroscop ... | 2012 | 22945941 |
| classification of grape berries according to diameter and total soluble solids to study the effect of light and temperature on methoxypyrazine, glutathione, and hydroxycinnamate evolution during ripening of sauvignon blanc (vitis vinifera l.). | grape berries were classified according to diameter and total soluble solids (tss) to study the effect of light and temperature on methoxypyrazines (mps), glutathione (gsh), and hydroxycinnamates (hcas) during the ripening of sauvignon blanc. the light exposure of the fruiting zone was modified within leaf and lateral removal at the phenological stage berry of peppercorn size and no removal (control). in comparison to the control, the concentration of 3-isobutyl-2-methoxypyrazine (ibmp) was belo ... | 2012 | 22946638 |
| genome-wide identification of viral and host transcripts targeted by viral sirnas in vitis vinifera. | in plants, rna silencing is a surveillance mechanism against invading viruses. it involves the production of virus-derived small interfering rnas (vsirnas), which guide the rna-induced silencing complex (risc) to inactivate viruses. vsirnas may also promote the silencing of host mrnas in a sequence-specific manner. in this work, vsirnas derived from two grapevine-infecting viruses (grapevine fleck virus and grapevine rupestris stem pitting-associated virus) were selected from cdna libraries of s ... | 2013 | 22947170 |
| the grapevine expression atlas reveals a deep transcriptome shift driving the entire plant into a maturation program. | we developed a genome-wide transcriptomic atlas of grapevine (vitis vinifera) based on 54 samples representing green and woody tissues and organs at different developmental stages as well as specialized tissues such as pollen and senescent leaves. together, these samples expressed ∼91% of the predicted grapevine genes. pollen and senescent leaves had unique transcriptomes reflecting their specialized functions and physiological status. however, microarray and rna-seq analysis grouped all the oth ... | 2012 | 22948079 |
| crystallization and preliminary x-ray characterization of the vitis vinifera fucokinase:gdp-fucose pyrophosphorylase. | the vitis vinifera dual-activity fucose and nucleotide-sugar metabolizing enzyme l-fucokinase:gdp-fucose pyrophosphorylase (fkp) has been purified to homogeneity and the 118.8 kda monomeric protein has been crystallized by vapor diffusion in zeppezauer tubes at 277 k. crystals of the apoenzyme diffracted to 2.6 å resolution and belonged to the tetragonal space group p4(1)2(1)2. there is a single fkp monomer in the asymmetric unit, giving a matthews coefficient of 3.22 å(3) da(-1) and a solvent c ... | 2012 | 22949207 |
| vvgols1 and vvhsfa2 are involved in the heat stress responses in grapevine berries. | among various environmental factors, temperature is a major regulator affecting plant growth, development and fruit composition. grapevine is the most cultivated fruit plant throughout the world, and grapes are used for wine production and human consumption. the molecular mechanisms involved in grapevine tolerance to high temperature, especially at the fruit level, are poorly understood. to better characterize the sensitivity of berries to the microenvironment, high temperature conditions were l ... | 2012 | 22952249 |
| copper transport and compartmentation in grape cells. | copper-based fungicides have been widely used against several grapevine (vitis vinifera l.) diseases since the late 1800s when the bordeaux mixture was developed, but their intensive use has raised phytotoxicity concerns. in this study, physiological, biochemical and molecular approaches were combined to investigate the impacts of copper in grape cells and how it is transported and compartmented intracellularly. copper reduced the growth and viability of grape cells (csb, cabernet sauvignon berr ... | 2012 | 22952251 |
| isorhapontigenin: a novel bioactive stilbene from wine grapes. | stilbenes are a family of bioactive compounds found in plants. however, only a few stilbenes are present in the human diet. grape and wine are the main dietary source of stilbenes, resveratrol and piceid being the most common ones. ultraviolet c light (uvc) postharvest treatment was used to obtain significantly increased stilbene concentration in grapes. a new, previously undescribed-in-grapes stilbene was found after uvc treatment. the process followed to isolate and identify this unknown stilb ... | 2012 | 22953865 |
| molecular cloning and functional analysis of two fad2 genes from american grape (vitis labrusca l.). | the synthesis of polyunsaturated fatty acids (pufas), the most abundant fatty acids in plants, begins with a reaction catalyzed by fatty acid desaturase 2 (fad2; ec 1.3.1.35), also called microsomal oleate δ12-desaturase. since the fad2 gene was first identified in arabidopsis thaliana, fad2 research has gained wide interest as the essential enzyme for synthesizing pufa. grapes are one of the most frequently cultivated fruits in the world, with most commercial growers cultivating vitis vinifera ... | 2012 | 22955034 |
| structural, functional, and evolutionary analysis of the unusually large stilbene synthase gene family in grapevine. | stilbenes are a small family of phenylpropanoids produced in a number of unrelated plant species, including grapevine (vitis vinifera). in addition to their participation in defense mechanisms in plants, stilbenes, such as resveratrol, display important pharmacological properties and are postulated to be involved in the health benefits associated with a moderate consumption of red wine. stilbene synthases (stss), which catalyze the biosynthesis of the stilbene backbone, seem to have evolved from ... | 2012 | 22961129 |
| free and bound aroma compounds characterization by gc-ms of negroamaro wine as affected by soil management. | negroamaro is an autochthonous wine grape variety of southern italy, which is becoming very important for the italian wine market. the wine aroma is primary affected by the chemical composition of grapes, which can be influenced also by agronomic practices such as soil management. in this study, the free and bound aroma characterization was performed by gas chromatography-mass spectrometry analyses, and the influence of two soil managements (cover cropping and soil tillage) was evaluated. a tota ... | 2012 | 22972778 |
| three years monitoring survey of pesticide residues in sardinia wines following integrated pest management strategies. | this paper reports the results of a pesticide monitoring survey on wine grapes from the 2008-2010 vintage from vineyards grown according to integrated pest management strategies. a multi-residue gas chromatography-mass spectrometry method in electron ionization and chemical ionization mode has been used for the determination of 30 pesticides in wine samples. the analytical method showed good recoveries and allowed a good separation of the selected pesticides. repeatability and intermediate preci ... | 2013 | 22976121 |
| varietal and pre-fermentative volatiles during ripening of vitis vinifera cv nebbiolo berries from three growing areas. | flavour analysis of grape is a key step in quality evaluation. the stir bar sorptive extraction technique (sbse, 'twister'®) was used to assess varietal and pre-fermentative volatile accumulation in 'nebbiolo' berries, from véraison to harvest. grapes were collected in three vineyards, representing different 'crus' in the cultivation areas of barolo, barbaresco and roero (north-west italy). volatile constituents of grapes were identified and quantified by gc-ms. we demonstrate the influence exer ... | 2012 | 22980811 |
| floral, spicy and herbaceous active odorants in gran negro grapes from shoulders and tips into the cluster, and comparison with brancellao and mouratón varieties. | within the framework of a more and more competitive market, the opportunity to obtain different wines from the same variety cultivated in the same vineyard is becoming of increasing importance. in this study the presence of aroma compounds in gran negro (vitis vinifera l.) grapes was investigated in order to obtain its aroma potential fingerprint taking into consideration the separation of apical (tips) and basal (shoulders) berries of the clusters. in the final stages of maturation, differences ... | 2012 | 22980871 |
| in silico identification of novel hevein-like peptide precursors. | lectins are proteins with ability to bind reversibly and non-enzymatically to a specific carbohydrate. they are involved in numerous biological processes and show enormous biotechnological potential. among plant lectins, the hevein domain is extremely common, being observed in several kinds of lectins. moreover, this domain is also observed in an important class of antimicrobial peptides named hevein-like peptides. due to higher cysteine residues conservation, hevein-like peptides could be mined ... | 2012 | 22981805 |
| comprehensive analysis of nac domain transcription factor gene family in vitis vinifera. | key message : genome-wide identification of grapevine nac domain genes and investigation of their chromosome locations, gene structures, duplication, evolution, phylogeny and expression profiles. grapevine is a widely used fruit crop. nac (nam, ataf1/2 and cuc2) domain genes are plant-specific transcription factors (tfs) that comprise a conserved nam domain in the n-terminus. members of this gene family have been reported to contribute to plant development. during this study, 74 nac genes were i ... | 2013 | 22983198 |
| genome-wide identification and analysis of the tify gene family in grape. | the tify gene family constitutes a plant-specific group of genes with a broad range of functions. this family encodes four subfamilies of proteins, including zml, tify, ppd and jasmonate zim-domain (jaz) proteins. jaz proteins are targets of the scf(coi1) complex, and function as negative regulators in the ja signaling pathway. recently, it has been reported in both arabidopsis and rice that tify genes, and especially jaz genes, may be involved in plant defense against insect feeding, wounding, ... | 2012 | 22984514 |
| tracing phenolic biosynthesis in vitis vinifera via in situ c-13 labeling and liquid chromatography-diode-array detector-mass spectrometer/mass spectrometer detection. | phenolic compounds in vitis vinifera contribute important flavor, functionality, and health qualities to both table and wine grapes. the plant phenolic metabolic pathway has been well characterized, however many important questions remain regarding the influence of environmental conditions on pathway regulation. as a diagnostic for this pathway's regulation, we present a technique to incorporate a stable-isotopic tracer, l-phenyl-(13)c(6)-alanine (phe(13)), into grape berries in situ and the acc ... | 2012 | 22986135 |
| computational analysis and characterization of uce-like elements (ules) in plant genomes. | ultraconserved elements (uces), stretches of dna that are identical between distantly related species, are enigmatic genomic features whose function is not well understood. first identified and characterized in mammals, uces have been proposed to play important roles in gene regulation, rna processing, and maintaining genome integrity. however, because all of these functions can tolerate some sequence variation, their ultraconserved and ultraselected nature is not explained. we investigated whet ... | 2012 | 22987666 |
| co-evolution between grapevine rupestris stem pitting-associated virus and vitis vinifera l. leads to decreased defence responses and increased transcription of genes related to photosynthesis. | grapevine rupestris stem pitting-associated virus (grspav) is a widespread virus infecting vitis spp. although it has established a compatible viral interaction in vitis vinifera without the development of phenotypic alterations, it can occur as distinct variants that show different symptoms in diverse vitis species. the changes induced by grspav in v. vinifera cv 'bosco', an italian white grape variety, were investigated by combining agronomic, physiological, and molecular approaches, in order ... | 2012 | 22987838 |
| posterior hyaloid detachment and internal limiting membrane peeling assisted by anthocyanins from acai fruit (euterpe oleracea) and 10 other natural vital dyes: experimental study in cadaveric eyes. | the purpose of this study was to determine whether natural dyes facilitate posterior hyaloid detachment (posterior vitreous detachment [pvd]) and retinal internal limiting membrane (ilm) peeling in human eyes. | 2013 | 22990318 |
| from rainfall to throughfall in a maritime vineyard. | this study deals with the characteristics of throughfall produced by vine (vitis vinifera l.) in one of the most common pedoclimatic conditions for grape production: a soil derived from marine sediments under a temperate mediterranean climate, and located rather close to the seacoast. to distinguish the contribution of the plant from that of the atmospheric deposition, the throughfall was collected for more than one year under real and artificial (plastic) vines; for the same period, also the bu ... | 2012 | 22995706 |
| enhancement of viniferin production in vitis vinifera l. cv. alphonse lavallée cell suspensions by low-energy ultrasound alone and in combination with methyl jasmonate. | this study examined for the first time the effect of low-energy ultrasound (us), used alone or in combination with methyl jasmonate (meja), on viniferin production in cell cultures of vitis vinifera l. cv alphonse lavallée. cell suspensions were exposed for 2 min to us (power 30, 60, and 90 mw cm(-3)). the highest viniferin production was obtained at 30 mw cm(-3). when sonication was performed twice, the effect on viniferin production was negligible, whereas triple sonication slightly increased ... | 2012 | 23004353 |