Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| differences between national reference laboratories of the european community in their ability to serotype salmonella species. | the capacity of national reference laboratories of the european union member states to correctly serotype salmonella strains was assessed in four collaborative studies on serotyping in the period 1995-1999. participants were asked to identify 20 strains in studies i, ii and iii and 16 strains in study iv, using the typing method routinely performed in their laboratory. in the first study, the strains to be identified belonged to salmonella enterica subsp. enterica, salamae or houtenae, while in ... | 2002 | 11957022 |
| lack of interaction of fluoroquinolones with lipopolysaccharides. | fluoroquinolones are known to chelate with di- and trivalent cations, and it has accordingly been claimed that they perturb the integrity of the outer membrane (om) of gram-negative bacteria. so far, chelation has not been assessed in biologically relevant test systems. therefore, we investigated the interaction of ciprofloxacin and moxifloxacin in the absence and presence of mg2+ with whole bacteria and isolated lipopolysaccharide (lps) from various rough mutant strains of salmonella enterica c ... | 2002 | 11959601 |
| characterization of a laboratory-derived, high-level ampicillin-resistant salmonella enterica serovar typhimurium strain that caused meningitis in an infant. | a salmonella enterica serovar typhimurium strain that harbored a plasmid carrying a tem-1-type beta-lactamase gene was isolated from the blood and cerebrospinal fluid of an infant with meningitis. this 3.2-kb plasmid was further characterized to be a nonconjugative pgem series cloning vector containing a foreign insert. the strain was likely laboratory derived and contaminated the environment before it caused the infection. | 2002 | 11959613 |
| molecular mechanisms of salmonella pathogenesis. | the success of a pathogen depends on its capacity to enter a host, circumvent host defense barriers and establish infection. the gram-negative bacterium salmonella enterica has evolved different strategies to subvert normal host cellular functions, which allow it to enter into and proliferate within host cells. | 2000 | 11964823 |
| global aspects of antimicrobial-resistant enteric bacteria. | antibiotics have been considered to be safe and effective 'magic bullets', with no disadvantages to their widespread use. this has been proven to be a complacent attitude, with ever-increasing prevalences of resistance now evident. the present review covers aspects of the development, mechanisms and genetics of antimicrobial resistance in enteric commensals and pathogens. | 2001 | 11964879 |
| salmonella enterica serotype typhimurium dt104 isolated from humans, united states, 1985, 1990, and 1995. | first isolated from an ill person in 1985, multidrug-resistant salmonella enterica serotype typhimurium dt104 emerged in the mid-1990s as a strain of salmonella frequently isolated from humans in the united states. we compared the integron content, plasmid profile, and xbai pulsed-field gel electrophoresis (pfge) patterns of multidrug-resistant s. typhimurium dt104 (mr-dt104) isolated from humans in the united states in 1985, 1990, and 1995. all isolates contained a 60-mda plasmid and had indist ... | 2002 | 11971772 |
| salmonella enterica serotype typhimurium dt 104 antibiotic resistance genomic island i in serotype paratyphi b. | we have identified salmonella genomic island i (sgi1) in an isolate of salmonella enterica serotype paratyphi b. this antibiotic-resistance gene cluster, which confers multidrug resistance, has been previously identified in s. enterica serotype typhimurium phage types dt 104 and dt 120 and in s. enterica serotype agona. | 2002 | 11971780 |
| multiply resistant (mr) salmonella enterica serotype typhimurium dt 12 and dt 120: a case of mr dt 104 in disguise? | multiresistant salmonella enterica serotype typhimurium definitive phage type (dt) 12 and dt 120 are more closely related to dt 104 than to non-multiresistant strains of their respective phage types. multiresistant dt 12 and dt 120 appear to have arisen due to changes in phage susceptibility of dt 104 rather than horizontal transfer of resistance genes. | 2002 | 11971781 |
| salmonella enterica serotype enteritidis phage type 4b outbreak associated with bean sprouts. | in november 2000 in the netherlands, an outbreak of salmonella enterica serotype enteritidis phage type 4b was investigated. eating bean sprouts was the only exposure associated with s. enteritidis pt 4b infection (matched odds ratio 13.0, 95% confidence interval 2.0-552.5). contaminated seeds were the most likely cause of contamination of the sprouts. the sprout grower applied a concentration of hypochlorite solution that was too low for seed disinfection. | 2002 | 11971783 |
| insertions in the anticodon loop of trna1gln(sufg) and trna(lys) promote quadruplet decoding of caaa. | base insertion mutations in the anticodons of two different escherichia coli trnas have been isolated that allow suppression of a series of +1 frameshift mutations. insertion of a u between positions 34 and 35 of trnagln1 or addition of a g between positions 36 and 37 of trna(lys) expand the anticodons of both trnas similarly to 3'-guuu(-5') and allow decoding of complementary 5'-caaa(-3') quadruplets. analysis of the suppressed mrna sequences suggests that suppression occurs by pairing of the e ... | 2002 | 11972336 |
| mg2+ homeostasis and avoidance of metal toxicity. | because mg2+ is required for a wide variety of cellular functions, its intracellular levels must be tightly regulated. in the gram-negative bacterium salmonella enterica, three transporters mediate mg2+ uptake: the p-type atpases mgta and mgtb, whose expression is transcriptionally induced in low mg2+ by the mg2+-regulated phop/phoq two-component system; and cora, whose transcription is regulated neither by the levels of mg2+ nor by the phop/phoq system. we now report that mutants defective in p ... | 2002 | 11972791 |
| salmonella enterica infections in market swine with and without transport and holding. | the objective of this study was to compare, by using identical sample types, the salmonella enterica prevalences and serovar diversities between pigs necropsied on the farm and those necropsied at the abattoir after transport and holding. we necropsied 567 market weight pigs (>70 kg) from six herds. pigs were alternately assigned to be necropsied on the farm or at the abattoir. one-half of the group was sent in clean, disinfected trailers to slaughter at a commercial abattoir. after transport (m ... | 2002 | 11976111 |
| the evolving genome of salmonella enterica serovar pullorum. | salmonella enterica serovar pullorum is a fowl-adapted bacterial pathogen that causes dysentery (pullorum disease). host adaptation and special pathogenesis make s. enterica serovar pullorum an exceptionally good system for studies of bacterial evolution and speciation, especially regarding pathogen-host interactions and the acquisition of pathogenicity. we constructed a genome map of s. enterica serovar pullorum rks5078, using i-ceui, xbai, avrii, and spei and tn10 insertions. pulsed-field gel ... | 2002 | 11976291 |
| identification of two prpdbc gene clusters in corynebacterium glutamicum and their involvement in propionate degradation via the 2-methylcitrate cycle. | genome sequencing revealed that the corynebacterium glutamicum genome contained, besides glta, two additional citrate synthase homologous genes (prpc) located in two different prpdbc gene clusters, which were designated prpd1b1c1 and prpd2b2c2. the coding regions of the two gene clusters as well as the predicted gene products showed sequence identities of about 70 to 80%. significant sequence similarities were found also to the prpbcde operons of escherichia coli and salmonella enterica, which a ... | 2002 | 11976302 |
| elevated levels of ketopantoate hydroxymethyltransferase (panb) lead to a physiologically significant coenzyme a elevation in salmonella enterica serovar typhimurium. | pantothenate is the product of the atp-dependent condensation of pantoate and beta-alanine and is a direct precursor of coenzyme a. a connection exists between pantothenate biosynthesis and thiamine biosynthesis in salmonella enterica serovar typhimurium since derivatives of a purf mutant that can grow (on glucose medium) in the absence of thiamine excrete pantothenate. we show here that the causative mutation in three such mutants was the addition of a cg base pair upstream of the panb gene. th ... | 2002 | 11976313 |
| two class ii d-tagatose-bisphosphate aldolases from enteric bacteria. | escherichia coli, salmonella enterica, klebsiella pneumoniaeand klebsiella oxytocawere found to contain two d-tagatose 1,6-bisphosphate (tagbp)-specific aldolases involved in catabolism of galactitol (genes gaty gatz) and of n-acetyl-galactosamine and d-galactosamine (genes kbay kbaz,also called agay agaz). the two aldolases were closely related (> or = 53.8% identical amino acids) and could substitute for each other in vivo. the catalytic subunits gaty or kbay alone were sufficient to show aldo ... | 2002 | 11976750 |
| isolation of salmonella enterica serotype worthington from a splenic abscess in a patient with chronic myeloid leukemia. | splenic abscesses are caused by staphylococcus aureus, streptococcus and bacteria belonging to the family enterobacteriaceae. we report a case of splenic abscess caused by an unusual serotype of salmonella. a 55 year old man was admitted with complaints of fever and abdominal pain. on the basis of clinical findings and laboratory reports, a diagnosis of chronic myeloid leukemia was made. ultrasonography of the abdomen revealed a single large cystic lesion in the spleen. percutaneous drainage of ... | 2003 | 11980609 |
| attenuated salmonella enterica serovar typhi live vector with inducible chromosomal expression of the t7 rna polymerase and its evaluation with reporter genes. | attenuated salmonella strains with defined gene deletions have been extensively evaluated as suitable live carriers of passenger antigens. a number of strategies for antigen delivery by these strains have been attempted, ranging from plasmid-based to chromosomal integration systems. we report here the chromosomal integration of the t7 rna polymerase gene (t7pol) in the attenuated strain salmonella enterica serovar typhi (salmonella typhi) cvd908 (aroc(-), arod(-)). the t7pol gene was amplified b ... | 2002 | 11982332 |
| invasive food poisoning caused by salmonella oranienburg. | 2002 | 11982885 | |
| bromoperoxidase activity of vanadate-substituted acid phosphatases from shigella flexneri and salmonella enterica ser. typhimurium. | vanadium haloperoxidases and the bacterial class a nonspecific acid phosphatases have a conserved active site. it is shown that vanadate-substituted recombinant acid phosphatase from shigella flexneri (phon-sf) and salmonella enterica ser. typhimurium (phon-se) in the presence of h2o2 are able to oxidize bromide to hypobromous acid. vanadate is essential for this activity. the kinetic parameters for the artificial bromoperoxidases have been determined. the km value for h2o2 is about the same as ... | 2002 | 11985594 |
| increasing quinolone resistance in salmonella enterica serotype enteritidis. | until recently, salmonella enterica serotype enteritidis has remained sensitive to most antibiotics. however, national surveillance data from denmark show that quinolone resistance in s. enteritidis has increased from 0.8% in 1995 to 8.5% in 2000. these data support concerns that the current use of quinolone in food animals leads to increasing resistance in s. enteritidis and that action should be taken to limit such use. | 2002 | 11996688 |
| [will the effectiveness of the immunization of chicks with live salmonella vaccines be affected by maternal antibodies?]. | in the progeny of breeder birds which had been vaccinated with live salmonella typhimurium and inactivated salmonella enteritidis vaccines, the caecal and systemic colonisation by a live salmonella enteritidis and a live salmonella typhimurium vaccine was studied. the efficacy of the oral immunisation of chicks from vaccinated and non-vaccinated breeders with a live salmonella enteritidis vaccine on day 1 of age was studied by an experimental challenge with salmonella enteritidis on day 30 of ag ... | 2002 | 11998364 |
| phage typing and pfge pattern analysis as tools for epidemiological surveillance of salmonella enterica serovar bovismorbificans infections. | some years ago, an increase in the number of sporadic cases and outbreaks of salmonellosis due to s. enterica serovar bovismorbificans was observed in several european countries including finland, sweden, england/wales, austria, and germany. in order to understand the recent spread of this serovar and to trace the route of infection back to its source, it was considered necessary to subtype s. bovismorbificans isolates. using phage typing (newly described here) and molecular fingerprinting (pfge ... | 2002 | 12002528 |
| is200 fingerprinting of salmonella enterica serotype abortusovis strains isolated in iran. | salmonella enterica serovar abortusovis is one of the most common pathogens responsible for abortion in sheep. in iran, the spread of abortusovis is highly dependent on the nomadic life style. in this study we performed is200 fingerprinting to identify the clonal lines circulating in iran. all the isolates contained 4 or 5 copies of the transposon and could be classified in 4 genotypes. a single genotype was highly prevalent and very likely it has circulated in iran since 1970. all the isolates ... | 2002 | 12002552 |
| composite integron array generated by insertion of an orf341-type integron within a tn21-like element. | two class 1 integrons, in-t1 and in-t2, were previously identified in incfi plasmids of salmonella enterica serotype typhimurium. molecular analysis revealed a close physical link between the two integrons. in-t1 is preceded by the transposase genes of tn21, whereas in-t2 is located downstream the 3'-conserved segment (3'-cs) of in-t1, in a head-to-tail configuration. in-t1 shows a peculiar sequence downstream the 3'-cs, containing an extended version of the open reading frame known as orf341 (r ... | 2002 | 12002644 |
| nucleotide and amino acid sequences of orit-tram-traj-tray-traa-tral regions and mobilization of virulence plasmids of salmonella enterica serovars enteritidis, gallinarum-pullorum, and typhimurium. | the virulence plasmid of salmonella enterica serovar gallinarum-pullorum (pspv) but not those of salmonella enterica serovars enteritidis (psev) and typhimurium (pstv) can be readily mobilized by an f or f-like conjugative plasmid. to investigate the reason for the difference, the orit-tram-traj-tray-traa-tral regions of the three salmonella virulence plasmids (psvs) were cloned and their nucleotide and deduced amino acid sequences were examined. the cloned fragments were generally mobilized mor ... | 2002 | 12003924 |
| argr-independent induction and argr-dependent superinduction of the astcadbe operon in escherichia coli. | for escherichia coli, growth in the absence of ammonia is termed nitrogen limited and results in the induction of genes that assimilate other nitrogen sources, a response mediated by sigma(54) and nitrogen regulator i (nr(i), also called ntrc). the astcadbe operon, which is required for growth with arginine as the sole nitrogen source, is moderately expressed during general nitrogen limitation and maximally expressed in the presence of arginine. the operon is also induced in stationary phase. pr ... | 2002 | 12003934 |
| fluoroquinolone resistance in salmonella serovars isolated from humans and food animals. | quinolone-resistant salmonella enterica usually contain a mutation in gyra within the region encoding the quinolone resistance determining region of the a subunit of dna gyrase. these mutations confer substitutions analogous to escherichia coli ser83-->phe and asp87-->gly or tyr, or a novel mutation resulting in ala119-->glu or val. mutations in gyrb are rare, and no mutations in parc or pare have been described. quinolone-resistant salmonella can also be cross-resistant to other agents includin ... | 2002 | 12007640 |
| characterisation of the diol dehydratase pdu operon of lactobacillus collinoides. | the three genes (pducde) encoding the diol dehydratase of lactobacillus collinoides were sequenced. they exhibited strong identities with the ddrabc and pducde genes of klebsiella oxytoca and salmonella enterica, respectively. these genes are part of a putative operon with at least four other genes. an eighth open reading frame was identified as homologous to the pocr gene (encoding the operon regulatory protein). although the enzyme was detected in exponential growth phase, pducde activity was ... | 2002 | 12007656 |
| development of a baculovirus expression system for soluble porcine tumor necrosis factor receptor type i and soluble porcine tumor necrosis factor receptor type i-igg fusion protein. | tumor necrosis factor-alpha (tnf-alpha) is a key mediator of inflammatory responses and gram-negative bacterial sepsis, but the role that it plays during salmonella enterica species bacterial infections in swine has not yet been elucidated. to facilitate studies on the role of tnf-alpha on the pathology associated with salmonella infections in pigs, recombinant soluble porcine tnf receptor type i (rsptnf-ri) and soluble tnf receptor type i fused to the fc region of porcine igg1 (rsptnf-ri-igg) w ... | 2002 | 12007891 |
| temperate phages in salmonella enterica serovar typhimurium: implications for epidemiology. | salmonella enterica serovar typhimurium is the most common salmonella serovar isolated from humans in australia. the most common definitive phage types (dt) include 9, 64 and 135. induction of lysogenic phages from dt 64 with mitomycin c followed by cesium chloride gradient purification, resulted in separation of two populations of phage particles. dna extracted from these particles that was digested with smai showed two distinct patterns of banding. transmission electron microscopy showed that ... | 2002 | 12008917 |
| modified pulsed-field gel electrophoresis method for dna degradation-sensitive salmonella enterica and escherichia coli strains. | a number of s. enterica and e. coli strains appeared sensitive to a rapid dna degradation during the course of pfge pattern analysis. this kind of dna degradation could not be stopped by intensive treatment with proteinase k, formalin treatment, or other modifications of the protocol for the isolation of intact chromosomal dna. however, the application of 100 microm thiourea into the running buffer gave rise to clear-cut pfge patterns and in turn to an overall typeability. | 2002 | 12008918 |
| cytokine expression by attenuated intracellular bacteria regulates the immune response to infection: the salmonella model. | attenuated salmonella strains have shown excellent efficacy as mucosal vaccine delivery systems. in the present report, several recombinant strains of salmonella enterica serovar typhimurium, engineered to express defined murine cytokines, were used to study their potential immunoregulatory capacity in the mouse model of typhoid fever. specifically, recombinant strains expressing il-2 (known as gidil2) or tnf-alpha (gidtnf) were compared with the parental, non-cytokine-secreting, strain (brd509) ... | 2002 | 12009571 |
| combination of pulsed-field gel electrophoresis (pfge) and single-enzyme amplified fragment length polymorphism (saflp) for differentiation of multiresistant salmonella enterica serotype typhimurium. | to assess the combined application of plasmid profile typing, pulsed-field gel electrophoresis (pfge) and pcr-based single-enzyme amplified fragment length polymorphism (saflp) for the differentiation of 18 multiresistant (mr) and one drug-sensitive strain of salmonella enterica serotype typhimurium from humans and food animals. | 2002 | 12010170 |
| sifa, a type iii secreted effector of salmonella typhimurium, directs salmonella-induced filament (sif) formation along microtubules. | a unique feature of salmonella enterica serovar typhimurium (s. typhimurium) is its ability to enter into (invade) epithelial cells and elongate the vacuole it occupies into tubular structures called salmonella-induced filaments (sifs). this phenotype is dependent on sifa, a salmonella virulence factor that requires the spi-2-encoded type iii secretion system for delivery into host cells. previous attempts to study sifa and other type iii secreted proteins have been limited by a lack of suitable ... | 2002 | 12010459 |
| the effect of genomic position on reversion of a lac frameshift mutation (laciz33) during non-lethal selection (adaptive mutation). | in a system described by cairns and foster, starvation of a particular leaky lac mutant (laciz33) in the presence of lactose appears to direct mutation in non-growing cells to sites that allow growth (adaptive mutation). this behaviour requires that the lac operon be located on an f' plasmid. this position effect was investigated by placing the mutant lac operon at many sites in the genome of salmonella enterica (typhimurium; lt2) and testing reversion behaviour. genomic position did not affect ... | 2002 | 12010495 |
| genetic diversity of salmonella enterica serovar paratyphi a from different geographical regions in asia. | subtyping of salmonella paratyphi a isolates from india, pakistan, indonesia and malaysia was carried out by pulsed-field gel electrophoresis (pfge) to assess the extent of genetic diversity of these isolates from different endemic countries. | 2002 | 12010557 |
| role of nitric oxide in host defense in murine salmonellosis as a function of its antibacterial and antiapoptotic activities. | host defense functions of nitric oxide (no) are known for many bacterial infections. in this study, we investigated the antimicrobial effect of no in murine salmonellosis by using inducible no synthase (inos)-deficient mice infected with an avirulent or virulent salmonella enterica serovar typhimurium strain. all inos-deficient mice died of severe septicemia within 6 days after intraperitoneal injection with an avirulent strain (lt2) to which wild-type mice were highly resistant; 50% lethal dose ... | 2002 | 12011007 |
| disruption of the salmonella-containing vacuole leads to increased replication of salmonella enterica serovar typhimurium in the cytosol of epithelial cells. | salmonella enterica serovar typhimurium is a facultative intracellular pathogen that inhabits a vacuolar compartment, called the salmonella-containing vacuole (scv), in infected host cells. maintenance of the scv is accomplished by sifa, and mutants of this salmonella pathogenicity island 2 type iii effector replicate more efficiently in epithelial cells. here we demonstrate that enhanced replication of sifa mutants occurs in the cytosol of these cells. increased replication of wild-type bacteri ... | 2002 | 12011022 |
| characterization of the spv locus in salmonella enterica serovar arizona. | salmonella enterica serovar arizona (s. enterica subspecies iiia) is a common salmonella isolate from reptiles and can cause serious systemic disease in humans. the spv virulence locus, found on large plasmids in salmonella subspecies i serovars associated with severe infections, was confirmed to be located on the chromosome of serovar arizona. sequence analysis revealed that the serovar arizona spv locus contains homologues of spvrabc but lacks the spvd gene and contains a frameshift in spva, r ... | 2002 | 12011028 |
| genomic organization of lps-specific loci. | 2002 | 12014174 | |
| factors affecting haemolysin production and congo red binding in salmonella enterica serovar typhimurium dt 98. | differences in haemolysin expression were observed in a strain of salmonella enterica serovar typhimurium definitive phage type (dt) 98 cultured under various conditions. haemolysin expression was optimal in cultures grown micro-aerobically. the zones of haemolysis were wider after longer periods of incubation. haemolysin production varied after growth in the following media (greatest to least): brain heart infusion (bhi) broth > nutrient broth (nb)>trypticase soy broth (tsb)> m-9 glucose medium ... | 2002 | 12018658 |
| characterization of variant salmonella genomic island 1 multidrug resistance regions from serovars typhimurium dt104 and agona. | strains of multidrug-resistant salmonella enterica serovar typhimurium dt104 (dt104) and s. enterica serovar agona (agona) have been found to harbor salmonella genomic island 1 (sgi1), a 43-kb genomic region that contains many of the drug resistance genes. such strains are resistant to ampicillin (pse-1), chloramphenicol/florfenicol (flor), streptomycin/spectinomycin (aada2), sulfonamides (sul1), and tetracycline [tet(g)] (commonly called the acssut phenotype). all five resistance genes are foun ... | 2002 | 12019080 |
| reducing mortality in salmonella enterica serovar typhimurium-infected mice with a tripeptidic serum fraction. | salmonellosis-induced mortality in female swiss webster mice decreased significantly when tripeptidic immunostimulant (tpi) was administered prophylactically. prophylactic benefits developed in a dose-dependent manner wherein 15 mg of tpi given 1 day before challenge reduced mortality by 70%. | 2002 | 12019117 |
| specific gyra mutation at codon 83 in nalidixic acid-resistant salmonella enterica serovar typhi strains isolated from vietnamese patients. | 2002 | 12019140 | |
| the sctr of salmonella enterica serova typhimurium encoding a homologue of merr protein is involved in the copper-responsive regulation of cuid. | we have identified the cuid gene in salmonella enterica serova typhimurium that codes for a putative multicopper oxidase. expression of cuid is induced by copper ion and its promoter/operator has sequence similarity to the promoters controlled by the transcriptional regulators of the merr family. we also identified and isolated a gene from s. enterica serova typhimurium that encodes a 138-amino acid residue protein, sctr, a new member of the merr family of transcriptional regulators. transposon- ... | 2002 | 12023084 |
| heterogeneity in expression of lipopolysaccharide by strains of salmonella enterica serotype typhimurium definitive phage type 104 and related phage types. | to investigate lipopolysaccharide (lps) expression in salmonella enterica serotype typhimurium definitive phage type 104 (salmonella typhimurium dt104) and related phage types. | 2002 | 12028424 |
| regulation of salmonella enterica serovar typhimurium mnth transcription by h(2)o(2), fe(2+), and mn(2+). | mnth, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (nramp1), is a primary transporter for mn(2+) influx in salmonella enterica serovar typhimurium and escherichia coli. s. enterica serovar typhimurium mnth contributes to h(2)o(2) resistance and is important for full virulence. consistent with its phenotype and function, mnth is regulated at the transcriptional level by both h(2)o(2) and substrate cation. we have now identified three trans-acting regulatory ... | 2002 | 12029030 |
| sitabcd is the alkaline mn(2+) transporter of salmonella enterica serovar typhimurium. | mnth, a bacterial homolog of the mammalian natural resistance-associated macrophage protein 1 (nramp1), is a primary mn(2+) transporter of salmonella enterica serovar typhimurium and escherichia coli. s. enterica serovar typhimurium mnth expression is important for full virulence; however, strains carrying an mnth deletion are only partially attenuated and display no obvious signs of mn(2+) deficiency. we noted that promoter sequences for mnth and for the putative fe(2+) transporter sitabcd appe ... | 2002 | 12029031 |
| mig-14 is a salmonella gene that plays a role in bacterial resistance to antimicrobial peptides. | it was previously demonstrated that the mig-14 gene of salmonella enterica serovar typhimurium is necessary for bacterial proliferation in the liver and spleen of mice following intragastric inoculation and that mig-14 expression, which is induced within macrophages, is under the control of the global regulator phop. here we demonstrate that the mig-14 promoter is induced by growth in minimal medium containing low magnesium or acidic ph, consistent with regulation by phop. in addition, mig-14 is ... | 2002 | 12029036 |
| substrate recognition properties of oligopeptidase b from salmonella enterica serovar typhimurium. | oligopeptidase b (opdb) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. we report here the cloning and expression of the opdb homologue from salmonella enterica serovar typhimurium and demonstrate that it exhibits amidolytic activity exclusively against substrates with basic residues in p(1). while similar to its eukaryotic ho ... | 2002 | 12029050 |
| genomewide identification of proteins secreted by the hrp type iii protein secretion system of pseudomonas syringae pv. tomato dc3000. | the ability of pseudomonas syringae pv. tomato dc3000 to be pathogenic on plants depends on the hrp (hypersensitive response and pathogenicity) type iii protein secretion system and the effector proteins it translocates into plant cells. through iterative application of experimental and computational techniques, the dc3000 effector inventory has been substantially enlarged. five homologs of known avirulence (avr) proteins and five effector candidates, encoded by genes with putative hrp promoters ... | 2002 | 12032338 |
| inorganic polyphosphate is essential for long-term survival and virulence factors in shigella and salmonella spp. | the importance of inorganic polyphosphate (poly p) and poly p kinase (ppk), the enzyme principally responsible for its synthesis, has been established previously for stationary-phase survival of escherichia coli and virulence in pseudomonas aeruginosa. the gene (ppk) that encodes ppk is highly conserved among many bacterial pathogens, including shigella and salmonella spp. in view of the phylogenetic similarity of the enteropathogens and the frequency with which virulence factors are expressed i ... | 2002 | 12032342 |
| microsatellites linked to salmonella enterica serovar enteritidis burden in spleen and cecal content of young f1 broiler-cross chicks. | contamination of poultry and poultry products by salmonella enterica serovar enteritidis (se) continues to be problematic even though biosafety management practices have aided in reduction of the se burden. identification of molecular markers linked to disease resistance loci would further reduce se burden by enabling selection for genetic resistance. the objectives of this study were therefore to evaluate specific genomic regions for resistance to se burden in young broiler-cross chicks and to ... | 2002 | 12033415 |
| profile of salmonella enterica subsp. enterica (subspecies i) serotype 4,5,12:i:- strains causing food-borne infections in new york city. | strains of newly emerging salmonella enterica subsp. enterica (subspecies i) serotype 4,5,12:i:- causing food-borne infections, including a large food poisoning outbreak (n = 86) characterized by persistent diarrhea (14% bloody), abdominal pain, fever, and headache, were examined. the organisms were found in the stool samples from the patients. the biochemical profile of the organisms is consistent with that of s. enterica subsp. i serotypes, except for decreased dulcitol (13%) and increased ino ... | 2002 | 12037044 |
| molecular subtyping methods for detection of salmonella enterica serovar oranienburg outbreaks. | this study involved 82 salmonella enterica serovar oranienburg isolates from patients with gastroenteritis and/or focal infections, healthy carriers, and cuttlefish chips which were epidemiologically linked to a major outbreak that had affected 1,505 people in japan between 1998 and 1999. we concurrently investigated four different molecular subtyping methods using human salmonellosis-associated salmonella serovars and their applicability in detection of serovar oranienburg in an outbreak. pulse ... | 2002 | 12037064 |
| dna microarray-based typing of an atypical monophasic salmonella enterica serovar. | a multidrug-resistant fljb-lacking salmonella enterica serovar [4,5,12:i:-] emerged in spain in 1997. we analyzed the genome from four strains of this serovar using a microarray containing almost all the predicted protein coding regions of serovar typhimurium strain lt2, including the pslt plasmid. only a few differences from serovar typhimurium lt2 were observed, suggesting the serovar to be typhimurium as well. six regions of interest were identified from the microarray data. cluster i was a d ... | 2002 | 12037067 |
| detection of plasmid-mediated ampc beta-lactamase genes in clinical isolates by using multiplex pcr. | therapeutic options for infections caused by gram-negative organisms expressing plasmid-mediated ampc beta-lactamases are limited because these organisms are usually resistant to all the beta-lactam antibiotics, except for cefepime, cefpirome, and the carbapenems. these organisms are a major concern in nosocomial infections and should therefore be monitored in surveillance studies. six families of plasmid-mediated ampc beta-lactamases have been identified, but no phenotypic test can differentiat ... | 2002 | 12037080 |
| salmonella enterica serovar typhimurium and escherichia coli contamination of root and leaf vegetables grown in soils with incorporated bovine manure. | bovine manure, with or without added salmonella enterica serovar typhimurium (three strains), was incorporated into silty clay loam (scl) and loamy sand (ls) soil beds (53- by 114-cm surface area, 17.5 cm deep) and maintained in two controlled-environment chambers. the s. enterica serovar typhimurium inoculum was 4 to 5 log cfu/g in manure-fertilized soil. the conditions in the two environmental chambers, each containing inoculated and uninoculated beds of manure-fertilized soil, simulated daily ... | 2002 | 12039728 |
| differences in growth of salmonella enterica and escherichia coli o157:h7 on alfalfa sprouts. | sprout producers have recently been faced with several salmonella enterica and escherichia coli o157:h7 outbreaks. many of the outbreaks have been traced to sprout seeds contaminated with low levels of human pathogens. alfalfa seeds were inoculated with s. enterica and e. coli o157:h7 strains isolated from alfalfa seeds or other environmental sources and sprouted to examine growth of these human pathogens in association with sprouting seeds. s. enterica strains grew an average of 3.7 log(10) on ... | 2002 | 12039774 |
| unusual salmonella enterica serotype typhimurium isolate producing cmy-7, shv-9 and oxa-30 beta-lactamases. | beta-lactam resistance in salmonella isolates is increasing. this paper describes the combination of three different beta-lactamases, oxa-30, shv-9 and cmy-7, expressed by an isolate of salmonella enterica serotype typhimurium. this is the first report of an isolate of salmonella having both an extended-spectrum beta-lactamase and an ampc beta-lactamase. | 2002 | 12039894 |
| thermodynamics of aminoglycoside and acyl-coenzyme a binding to the salmonella enterica aac(6')-iy aminoglycoside n-acetyltransferase. | kinetic and mechanistic studies on the chromosomally encoded aminoglycoside 6'-n-acetyltransferase, aac(6')-iy, of salmonella enterica that confers resistance toward aminoglycosides have been previously reported [magnet et al. (2001) biochemistry 40, 3700-3709]. in the present study, equilibrium binding and the thermodynamic parameters of binding of aminoglycosides and acyl-coenzyme a derivatives to aac(6')-iy and of two mutants, c109a and the c109a/c70a double mutant, have been studied using fl ... | 2002 | 12044186 |
| enhanced antimicrobial effects of combination of lactate and diacetate on listeria monocytogenes and salmonella spp. in beef bologna. | the antimicrobial activities of salts of organic acids such as lactate and acetate are well documented, but there is limited information on their effect when used in combination. we previously reported enhanced inhibition of listeria monocvtogenes and salmonella enterica serovar enteritidis in sterile comminuted beef at 5 and 10 degrees c by combinations of sodium lactate (sl) (2.5%) and sodium diacetate (sda) (0.2%). the present study was undertaken to evaluate the inhibitory effect of these sa ... | 2002 | 12051475 |
| prevalence of escherichia coli o157:h7 prophage-like sequences among german salmonella enterica serotype typhimurium phage types and their use in detection of phage type dt104 by the polymerase chain reaction. | a 1.6kb dna fragment identified by random amplifiable polymorphic dna differentiation (rapd) from a salmonella enterica serotype typhimurium phage type dt104 isolate was used to investigate the prevalence of the region in 160 dt104 isolates, 83 other epidemiological important s. typhimurium phage types and 20 strains selected from 17 other salmonella serotypes. pcr screening tests using two different primer-sets derived from the rapd fragment's nucleotide sequence showed that 76% of the 160 dt10 ... | 2002 | 12052335 |
| effect of fermented feed on shedding of enterobacteriaceae by fattening pigs. | epidemiological studies showed that the use of fermented feed could significantly reduce salmonella prevalence in pigs compared to the use of normal feed. experimental challenge experiments with salmonella have however never been conducted to reveal the efficacy of fermented feed in reducing salmonella shedding and/or reducing the number of salmonella-positive pigs. a longitudinal study was conducted to measure the effect of fermented feed, in particular of its components lactic acid and lactoba ... | 2002 | 12052336 |
| monoclonal antibody of igg isotype against a cross-reactive lipopolysaccharide epitope of chlamydia and salmonella re chemotype enhances infectivity in l-929 fibroblast cells. | a murine monoclonal antibody (mab) 202d7 of igg3 isotype recognizes a lipopolysaccharide (lps) epitope of chlamydia spp. and cross-reacts with the re chemotype lps of salmonella and escherichia coli. the antibody exhibits strong complement activating properties and stimulates phagocytosis of salmonella enterica serovar minnesota re mutant by murine macrophages. salmonella re mutants are non-invasive for cell monolayers but still can enter and replicate in l-929 murine fibroblast cells. the entry ... | 2002 | 12052561 |
| antimicrobial resistance in salmonella isolated from animals and their environment in england and wales from 1988 to 1999. | resistance to 16 antimicrobial agents was monitored in 109,125 salmonella cultures isolated from animals, their environment and feedstuffs between 1988 and 1999. the sensitivity of the 6512 isolates of salmonella enterica enterica serotype dublin to all the antimicrobial agents tested varied from 98.2 per cent in 1997 to 99.7 per cent in 1990 and 1996. in contrast, among 28,053 isolates of salmonella enterica enterica serotype typhimurium, there was a marked decrease in their sensitivity to all ... | 2002 | 12054133 |
| early cytokine response of gnotobiotic piglets to salmonella enterica serotype typhimurium. | cytokine response against salmonella typhimurium is traditionally studied in conventional animals. germ-free animals, however, enable to study response against infection without background effect of other microorganisms. plasma and ileal inflammatory cytokines in germ-free piglets orally infected with virulent lt2 strain or, with a non-virulent sf1591 rough mutant were quantified by elisa. in plasma and ileal washes, ifn-gamma levels significantly increased in both infected groups. tnf-alpha and ... | 2002 | 12056480 |
| variation on a theme of sdr. dtdp-6-deoxy-l- lyxo-4-hexulose reductase (rmld) shows a new mg2+-dependent dimerization mode. | dtdp-6-deoxy-l-lyxo-4-hexulose reductase (rmld) catalyzes the final step in the conversion of dtdp-d-glucose to dtdp-l-rhamnose in an nad(p)h- and mg2+-dependent reaction. l-rhamnose biosynthesis is an antibacterial target. the structure of rmld from salmonella enterica serovar typhimurium has been determined, and complexes with nadh, nadph, and dtdp-l-rhamnose are reported. rmld differs from other short chain dehydrogenases in that it has a novel dimer interface that contains mg2+. enzyme catal ... | 2002 | 12057193 |
| pfs-dependent regulation of autoinducer 2 production in salmonella enterica serovar typhimurium. | bacterial intercellular communication provides a mechanism for signal-dependent regulation of gene expression to promote coordinated population behavior. salmonella enterica serovar typhimurium produces a non-homoserine lactone autoinducer in exponential phase as detected by a vibrio harveyi reporter assay for autoinducer 2 (ai-2) (m. g. surette and b. l. bassler, proc. natl. acad. sci. usa 95:7046-7050, 1998). the luxs gene product mediates the production of ai-2 (m. g. surette, m. b. miller, a ... | 2002 | 12057938 |
| differential regulation of multiple proteins of escherichia coli and salmonella enterica serovar typhimurium by the transcriptional regulator slya. | slya is a transcriptional regulator of escherichia coli, salmonella enterica, and other bacteria belonging to the enterobacteriaceae: the slya protein has been shown to be involved in the virulence of s. enterica serovar typhimurium, but its role in e. coli is unclear. in this study, we employed the proteome technology to analyze the slya regulons of enteroinvasive e. coli (eiec) and salmonella serovar typhimurium. in both cases, comparative analysis of the two-dimensional protein maps of a wild ... | 2002 | 12057949 |
| activation of prophage eib genes for immunoglobulin-binding proteins by genes from the ibrab genetic island of escherichia coli ecor-9. | four distinct escherichia coli immunoglobulin-binding (eib) genes, each of which encodes a surface-exposed protein that binds immunoglobulins in a nonimmune manner, are carried by separate prophages in e. coli reference (ecor) strain ecor-9. each eib gene was transferred to test e. coli strains, both in the form of multicopy recombinant plasmids and as lysogenized prophage. the derived lysogens express little or no eib protein, in sharp contrast to the parental lysogen, suggesting that ecor-9 ha ... | 2002 | 12057959 |
| characterization of salmonella enterica derivatives harboring defined aroc and salmonella pathogenicity island 2 type iii secretion system (ssav) mutations by immunization of healthy volunteers. | the attenuation and immunogenicity of two novel salmonella vaccine strains, salmonella enterica serovar typhi (ty2 delta aroc delta ssav, designated zh9) and s. enterica serovar typhimurium (tml delta aroc delta ssav, designated wt05), were evaluated after their oral administration to volunteers as single escalating doses of 10(7), 10(8), or 10(9) cfu. zh9 was well tolerated, not detected in blood, nor persistently excreted in stool. six of nine volunteers elicited anti-serovar typhi lipopolysac ... | 2002 | 12065485 |
| md-2, a novel accessory molecule, is involved in species-specific actions of salmonella lipid a. | salmonella lipid a is inactive in human macrophages despite being potently active in murine macrophages. we investigated the molecular basis for this species-specific action of salmonella lipid a. when murine cd14 (mcd14), mtlr4, and mmd-2 were all expressed in human monocytic thp-1 cells, these cells were capable of responding to salmonella lipid a. expressing each of these proteins separately did not impart such responsiveness. expression of mtlr4 plus mmd-2, but not mcd14 plus mtlr4 or mcd14 ... | 2002 | 12065494 |
| expression of the plasmodium falciparum immunodominant epitope (nanp)(4) on the surface of salmonella enterica using the autotransporter misl. | gram-negative bacterial proteins which are exported from the cytosol to the external environment by the type v secretion system are also known as autotransporters. once translocated to the periplasmic compartment by the sec-dependent general secretory pathway, their c-terminal domain forms a pore through which the n-terminal domain travels to the outer membrane without the need of other accessory proteins. misl (protein of membrane insertion and secretion) is a protein of unknown function locate ... | 2002 | 12065502 |
| the salmonella enterica serotype typhimurium effector proteins sipa, sopa, sopb, sopd, and sope2 act in concert to induce diarrhea in calves. | salmonella enterica serotype typhimurium requires a functional type iii secretion system encoded by salmonella pathogenicity island 1 (spi1) to cause diarrhea. we investigated the role of genes encoding secreted target proteins of the spi1-associated type iii secretion system for enteropathogenicity in calves. salmonella serotype typhimurium strains having mutations in sptp, avra, ssph1, or slrp induced fluid secretion in the bovine ligated ileal loop model at levels similar to that of the wild ... | 2002 | 12065528 |
| conjugal transfer of the virulence plasmid of salmonella enterica is regulated by the leucine-responsive regulatory protein and dna adenine methylation. | host-encoded functions that regulate the transfer operon (tra) in the virulence plasmid of salmonella enterica (pslt) were identified with a genetic screen. mutations that decreased tra operon expression mapped in the lrp gene, which encodes the leucine-responsive regulatory protein (lrp). reduced tra operon expression in an lrp- background is caused by lowered transcription of the traj gene, which encodes a transcriptional activator of the tra operon. gel retardation assays indicated that lrp b ... | 2002 | 12067346 |
| structural characterization of lipo-oligosaccharide (los) from yersinia pestis: regulation of los structure by the phopq system. | the two-component regulatory system phopq has been shown to regulate the expression of virulence factors in a number of bacterial species. for one such virulence factor, lipopolysaccharide (lps), the phopq system has been shown to regulate structural modifications in salmonella enterica var typhimurium. in yersinia pestis, which expresses lipo-oligosaccharide (los), a phopq regulatory system has been identified and an isogenic mutant constructed. to investigate potential modifications to los fro ... | 2002 | 12067350 |
| optimization of gfp levels for analyzing salmonella gene expression during an infection. | green fluorescent protein (gfp) is an attractive reporter for salmonella gene expression analysis but might interfere with virulence when expressed at high levels. to identify suitable gfp levels, we constructed a series of salmonella strains expressing different amounts of gfp and measured their fluorescence and colonization levels in infected mice. the results show that gfp concentrations in the range of 7000-200,000 molecules per salmonella cell are detectable in ex vivo samples using flow cy ... | 2002 | 12067736 |
| autoinduction of the ompr response regulator by acid shock and control of the salmonella enterica acid tolerance response. | salmonella enterica serovar typhimurium periodically experiences acid stress in a variety of host and non-host environments. an encounter with non-lethal acid stress (ph > 4) induces an assortment of physiological changes, called the acid tolerance response (atr), that helps the cell to tolerate extreme low ph (ph 3). these physiological changes differ in log phase and stationary phase cells and are controlled by different regulatory proteins. ompr is an acid-induced response regulator critical ... | 2002 | 12068808 |
| pathogenesis of infections with salmonella enterica subsp. enterica serovar muenchen in the turtle trachemys scripta scripta. | the pathogenesis of salmonella enterica subsp. enterica serovar muenchen infections in the aquatic turtle trachemys scripta scripta was studied. after oral infection with 5x10(5)cfu of serovar muenchen of 10-14-month-old turtles, kept at 26 degrees c, the intestine and especially the ileum, caecum and colon was colonized. invasion of the intestinal wall, causing histopathological lesions, and colonization of internal organs were not observed. serovar muenchen was only isolated from turtles for 8 ... | 2002 | 12069769 |
| risk factors for clinical salmonella enterica subsp. enterica serovar typhimurium infection on dutch dairy farms. | risk factors for outbreaks in 1999 of clinical salmonella enterica subsp. enterica serovar typhimurium infection on dairy farms were studied in a matched case-control study with 47 case farms and 47 control farms. all 47 case farms experienced a clinical outbreak of salmonellosis which was confirmed with a positive bacteriologic culture for serovar typhimurium in one or more samples. serovar typhimurium phage type 401 and 506 (definitive type 104, dt104) were the most frequently isolated phage t ... | 2002 | 12069778 |
| novel class 1 integron (ins21) carrying blactx-m-2 in salmonella enterica serovar infantis. | the genetic organization of the region coding for ctx-m-2 in salmonella enterica serovar infantis was determined by pcr mapping. this gene seems to have been mobilized from the kluyvera ascorbata chromosome to a complex suli-type integron, similar to in6 and in7. | 2002 | 12069984 |
| comparisons of sampling procedures and time of sampling for the detection of salmonella in danish infected chicken flocks raised in floor systems. | bacteriological follow-up samples were taken from 41 chicken (gallus gallus) flocks in floor systems, where salmonella enterica (salmonella) had been detected either directly in bacteriological samples or indirectly by serological samples. three types of follow-up samples were compared to each other within each flock: 1) 5 pairs of socks, analysed as 5 samples, 2) 2 pairs of socks, analysed as one sample, and 3) 60 faecal samples, analysed as one pooled sample. agreement between sampling methods ... | 2002 | 12071113 |
| evolutionary genomics of salmonella: gene acquisitions revealed by microarray analysis. | the presence of homologues of salmonella enterica sv. typhimurium lt2 genes was assessed in 22 other salmonella including members of all seven subspecies and salmonella bongori. genomes were hybridized to a microarray of over 97% of the 4,596 annotated orfs in the lt2 genome. a phylogenetic tree based on homologue content, relative to lt2, was largely concordant with previous studies using sequence information from several loci. based on the topology of this tree, homologues of genes in lt2 acqu ... | 2002 | 12072558 |
| polynucleotide phosphorylase is a global regulator of virulence and persistency in salmonella enterica. | for many pathogens, the ability to regulate their replication in host cells is a key element in establishing persistency. here, we identified a single point mutation in the gene for polynucleotide phosphorylase (pnpase) as a factor affecting bacterial invasion and intracellular replication, and which determines the alternation between acute or persistent infection in a mouse model for salmonella enterica infection. in parallel, with microarray analysis, pnpase was found to affect the mrna levels ... | 2002 | 12072563 |
| molecular tools for epidemiological investigations of s. enterica subspecies enterica infections. | salmonella infection is one of the most prevalent reported food-borne diseases in industrialised countries, most often associated with eating contaminated eggs, poultry and pork. traditionally, epidemiological investigations for salmonella enterica have been based on phenotypic characteristics. however, the predominance of certain phenotypes within hosts or locations makes further epidemiological subgrouping necessary. the combination of conventional and molecular epidemiology data is yielding i ... | 2002 | 12076110 |
| passage in mice causes a change in the ability of salmonella enterica serovar oranienburg to survive nacl osmotic stress: resuscitation from the viable but non-culturable state. | an outbreak caused by dried processed squids contaminated with salmonella oranienburg occurred in japan in 1999. isolates obtained from the causative food were resistant to nacl osmotic stress, but isolates from the patients were sensitive to nacl. although strains from both sources were almost identical in their virulence in mice, a nacl-resistant strain from food (sa9911t) became nacl-sensitive after passage in mice and a nacl-sensitive strain from one patient (sa99004) retained nacl sensitivi ... | 2002 | 12076792 |
| pulsed field gel electrophoresis for animal salmonella enterica serovar typhimurium isolates in taiwan. | salmonella enterica subspecies enterica serovar typhimurium is a common pathogen for humans and animals. in order to trace the clonal relationship and to find the circulating strains between human and animal isolates, chromosomal dnas from 87 serovar typhimurium strains isolated from animals (pigs were the majority) were subjected to xbai and spei digestion and pulsed field gel electrophoresis (pfge). for the 87 animal isolates, 38 pfge pattern combinations were obtained. as the subtyping result ... | 2002 | 12079748 |
| the atp:co(i)rrinoid adenosyltransferase (coba) enzyme of salmonella enterica requires the 2'-oh group of atp for function and yields inorganic triphosphate as its reaction byproduct. | the specificity of the atp:corrinoid adenosyltransferase (coba) enzyme of salmonella enterica serovar typhimurium lt2 for its nucleotide substrate was tested using atp analogs and alternative nucleotide donors. the enzyme showed broad specificity for the nucleotide base and required the 2'-oh group of the ribosyl moiety of atp for activity. (31)p nmr spectroscopy was used to identify inorganic triphosphate (ppp(i)) as the byproduct of the reaction catalyzed by the coba enzyme. cleavage of tripho ... | 2002 | 12080060 |
| a salmonella enterica serovar typhimurium hema mutant is highly susceptible to oxidative dna damage. | the first committed step in the biosynthesis of heme, an important cofactor of two catalases and a number of cytochromes, is catalyzed by the hema gene product. salmonella enterica serovar typhimurium hema26::tn10d (hema26) was identified in a genetic screen of insertion mutants that were sensitive to hydrogen peroxide. here we show that the hema26 mutant respires at half the rate of wild-type cells and is highly susceptible to the effects of oxygen species. exposure of the hema26 strain to hydr ... | 2002 | 12081946 |
| fitness of salmonella enterica serovar thompson in the cilantro phyllosphere. | the epiphytic fitness of salmonella enterica was assessed on cilantro plants by using a strain of s. enterica serovar thompson that was linked to an outbreak resulting from cilantro. salmonella serovar thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning microscopy (clsm) at high densities on the veins and in natural lesions. the population sizes of two common colonizers of plant surfaces, pantoea agglomerans and pseudomonas chloro ... | 2002 | 12089050 |
| characterization of salmonella serovars by pcr-single-strand conformation polymorphism analysis. | pcr-restriction fragment length polymorphism (pcr-rflp) and pcr-single-strand conformation polymorphism (pcr-sscp) analyses were carried out on the 1.6-kb groel gene from 41 strains of 10 different salmonella serovars. three haeiii rflp profiles were recognized, but no discrimination between the serovars could be achieved by this technique. however, pcr-sscp analysis of the groel genes of various salmonella serovars produced 14 sscp profiles, indicating the potential of this technique to differe ... | 2002 | 12089246 |
| genetic diversity of clinical and environmental strains of salmonella enterica serotype weltevreden isolated in malaysia. | the incidence of food-borne salmonellosis due to salmonella enterica serotype weltevreden is reported to be on the increase in malaysia. the pulsed-field gel electrophoresis (pfge) subtyping method was used to assess the extent of genetic diversity and clonality of salmonella serotype weltevreden strains from humans and the environment. pfge of xbai-digested chromosomal dna from 95 strains of salmonella serotype weltevreden gave 39 distinct profiles with a wide range of dice coefficients (0.27 t ... | 2002 | 12089269 |
| multidrug and broad-spectrum cephalosporin resistance among salmonella enterica serotype enteritidis clinical isolates in southern italy. | from 1992 to 1997, only six sporadic isolates of salmonella enterica serotype enteritidis from patients with cases of gastroenteritis in southern italy exhibited resistance to broad-spectrum cephalosporins. five isolates produced shv-12, and one isolate encoded a class c beta-lactamase. the bla(shv-12) gene was located in at least two different self-transferable plasmids, one of which also carried a novel class 1 integron. | 2002 | 12089302 |
| resistance mechanism for a number of non-typhoidic salmonella strains isolated in iaşi, romania. | the aim of this study was to find a possible explanation for the resistance pattern of the selected strains and to evaluate the impact in case of isolating such strains. during the period february-march 1998, in the pediatric hospital "sf. maria" from iasi, romania, 12 non-typhoidic salmonella strains, non-duplicates were isolated from stools of newborns and, in one case, from mother as well. identification was done by standard methods, and sensitivity testing by disk diffusion method, against t ... | 2000 | 12089972 |
| pore formation and function of phosphoporin phoe of escherichia coli are determined by the core sugar moiety of lipopolysaccharide. | the lipid matrix of the outer membrane of gram-negative bacteria is an asymmetric bilayer composed of a phospholipid inner leaflet and a lipopolysaccharide outer leaflet. incorporated into this lipid matrix are, among other macromolecules, the porins, which have a sieve-like function for the transport or exclusion of hydrophilic substances. it is known that a reduced amount of porins is found in the outer membrane of rough mutants as compared with wild-type bacteria. this observation was discuss ... | 2002 | 12091383 |
| multiresistant salmonella enterica serovar newport. | 2002 | 12092626 | |
| salmonella serotypes isolated from nonhuman sources in são paulo, brazil, from 1996 through 2000. | a total of 4,581 salmonella strains isolated from nonhuman sources, including foodstuffs associated with foodborne salmonella outbreaks, from january 1996 through december 2000 were serotyped at the enteropathogens laboratory, instituto adolfo lutz, são paulo, brazil. among the 123 different serotypes identified, salmonella enterica subsp. enterica serotype enteritidis (salmonella enteritidis) was the most prevalent (32.7%), ranking first for almost every kind of source. the next most common ser ... | 2002 | 12092719 |
| widal test in diagnosis of typhoid fever in turkey. | we studied the value of the widal tube agglutination test for the diagnosis of typhoid fever. the subjects were all adults >18 years of age and were divided into four groups: (i) 317 healthy blood donor controls, (ii) 31 bacteriologically confirmed patients with salmonella enterica serotype typhi, (iii) 21 patients with a clinical diagnosis of typhoid fever, and (iv) 41 febrile nontyphoid patients. blood donor controls were screened with a slide agglutination test for the salmonella enterica ser ... | 2002 | 12093703 |