Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| human papillomavirus type 16 dna expresses a replication modulation factor in cos-1 cells. | plasmid dna including the simian virus 40 (sv40) origin of replication undergoes uncontrolled, runaway replication in cos-1 cells owing to the intracellular production of sv40 large t antigen. covalent linkage of such plasmids with human papillomavirus type 16 (hpv-16) dna was found to prevent runaway replication. replication control was found to be dependent on the presence of hpv-16 dna sequences including the e1 open reading frame and part of the non-coding region. | 1990 | 2167937 |
| detection and typing of human papillomavirus in archival cervical cancer specimens by dna amplification with consensus primers. | we developed a polymerase chain reaction dna amplification system using two distinct consensus oligonucleotide primer sets for the improved detection and typing of a broad spectrum of human genital papillomavirus (hpv) sequences, including those of novel viruses. the system incorporates one primer set designed to amplify a highly conserved l1 domain and a second primer set designed to amplify a domain within the e6 gene. we used this system to analyze 48 fixed, paraffin-embedded tissue sections ... | 1990 | 2167986 |
| presence of human papillomavirus type-6-related sequences in inverted nasal papillomas. | twenty dna samples obtained from seven cases of inverted papillomas, eight cases of nasal polyps and five cases of chronic sinusitis were investigated by southern blot hybridization for the possible presence of sequences homologous to human papillomavirus (hpv) types 6, 11, 16 and 18. hpv type-6-related dna was identified in one of the seven inverted papillomas. the restriction endonuclease cleavage patterns showed that this latter dna is a new subtype of hpv type 6 dna. in the other six papillo ... | 1990 | 2168185 |
| detection of five different human papillomavirus types in cervical lesions by in situ hybridization. a study of 110 cases using sulfonated probes. | several findings suggest an etiologic relationship between genital tract squamous cell carcinoma and certain types of human papillomavirus (hpv). detection of these hpv types in cervical lesions considered as preneoplastic states (ie, cervical intraepithelial neoplasia or cin) is extremely important but difficult because the morphology of these states is highly heterogeneous and clinical course is rarely predictable. in situ hybridization (ish) is the only technique allowing correlation between ... | 1990 | 2168337 |
| [the role of human papillomavirus in the pathogenesis of papillomas of the upper aero-digestive tract]. | the term 'papilloma' is used to describe a highly diverse group of exophytic pathologic lesions in the upper aero-digestive tract. these lesions differ according to anatomic site, constituent epithelium and endoscopic appearance. the varying pathogenesis of papillomas is incompletely understood, but appears to be related both to host factors, e.g. regional differences in the susceptibility of epithelial cells, and to a number of cofactors. among the cofactors already identified, the human papill ... | 1990 | 2168577 |
| langerhans cells and epithelial cell modifications in cervical intraepithelial neoplasia: correlation with human papillomavirus infection. | the study of a series of 18 cervical intraepithelial neoplasia (cin) grade ii and iii was aimed at determining the distribution and phenotype of immunocompetent cells (langerhans cells, t and nk cells) and the alteration in the expression of egf receptors and beta 2-microglobulin in correlation with human papillomavirus (hpv) infection (viral antigen and dna typing with biotinylated probes). these lesions were characterized by a reduced number of langerhans cells and a dense infiltrate. hpv infe ... | 1990 | 2168858 |
| suppression in vivo of human papillomavirus type 18 e6-e7 gene expression in nontumorigenic hela x fibroblast hybrid cells. | the e6 and e7 genes of the cancer-associated human papillomavirus (hpv) types 16 (hpv16) and 18 (hpv18) can induce cell immortalization in vitro in normal human keratinocytes. this, however, is not associated with tumorigenicity in vivo. on the other hand, tumorigenicity of hpv18-positive hela cervical carcinoma cells can be suppressed by fusion of hela cells with normal human keratinocytes or fibroblasts. we have addressed the question of whether suppression of tumorigenicity in hela x fibrobla ... | 1990 | 2168962 |
| transforming growth factors beta 1 and 2 transcriptionally regulate human papillomavirus (hpv) type 16 early gene expression in hpv-immortalized human genital epithelial cells. | human papillomavirus type 16 (hpv16) early proteins e6 and e7 have been implicated in maintenance of the malignant phenotype in cervical cancer. transforming growth factors beta one and two (tgf betas 1 and 2), polypeptides that regulate cellular growth and differentiation, reversibly inhibited expression of the hpv16 e6 and e7 genes in several immortal genital epithelial cell lines. loss of e6 and e7 protein expression followed a dramatic time- and dose-dependent decrease in e6 and e7 rna level ... | 1990 | 2168964 |
| a member of the activator protein 1 family found in keratinocytes but not in fibroblasts required for transcription from a human papillomavirus type 18 promoter. | papillomaviruses are tissue specific and replicate in differentiating keratinocytes. we are interested in the question of tissue specificity at the level of transcription. we used extracts from human keratinocytes and human fibroblasts at low passage number and from hela cells to look for factors binding to the e6 promoter of human papillomavirus type 18 (hpv-18) dna by footprint and gel mobility shift experiments. we found a factor present in hela and keratinocyte extracts but not in fibroblast ... | 1990 | 2168967 |
| human papillomavirus type 6 long control region and human cellular dna contain related sequences. | we have identified a region of human papillomavirus type 6 (hpv-6) dna that hybridizes with human cellular dna containing no detectable hpv dna sequences. the region of hybridization has been localized to a segment of the viral long control region between the end of the l1 open reading frame and the late polyadenylation signal and is likely contained within a 94-base-pair insertion at nucleotide 7350 which is present in the cloned hpv-6b dna used for these studies. restriction fragments of hpv-6 ... | 1990 | 2168994 |
| detection of human papillomavirus and epstein-barr virus dna sequences in oral mucosa of hiv-infected patients by the polymerase chain reaction. | the presence of human papillomavirus (hpv) and epstein-barr virus (ebv) was analyzed in 21 oral biopsy specimens of hiv-infected patients using the polymerase chain reaction (pcr) method. biopsies were categorized as hairy leukoplakia (hl) (n = 12), candidiasis (n = 3), oral warts (n = 2), and clinically normal epithelium (n = 4). for hpv detection a modified general primer-mediated pcr method (gp-pcr), which detects a broad spectrum of hpv genotypes at sub-picogram levels, was used. human papil ... | 1990 | 2169191 |
| human papillomavirus transmission and carcinogenesis. | 1990 | 2169235 | |
| detection of human papilloma virus dna sequences by polymerase chain reaction. | we have developed a polymerase chain reaction-based procedure for reproducible detection of the e6-e7 gene in human papilloma virus dna sequences using formalin-fixed, paraffin-embedded tissue sections. this procedure is a simple one-step procedure which does not require any elaborate hybridization following polymerase chain reaction amplification. the protocol combines modified tissue treatment and proper primer selection for efficient amplification of target dna in a highly specific manner all ... | 1990 | 2169264 |
| squamous cell carcinoma of the nail bed: a clinicopathological study of 12 cases. | twelve cases were reviewed of squamous cell carcinoma of the nail bed, with emphasis on the aetiological role of human papillomavirus (hpv) infection and radiation damage. using a hybridization technique, similar hpv genomes were detected in a uterine cervical neoplasia and a subungual squamous cell carcinoma in the same patient. | 1990 | 2169297 |
| [diagnosis and follow-up study of human papillomavirus infection in benign epithelium and mild dysplasia of the uterine cervix]. | in this study, we evaluated the sensitivity of cytology, colposcopy and the dot blot method (vira pap, vira type) for the screening of hpv infection in benign epithelium and mild dysplasia of the uterine cervix, and then investigated subsequent changes in hpv dna in the lesion. the hpv infection detection rates for cytology, colposcopy and the dot blot method were 14/46 (30.4%), 30/46 (65.2%) and 35/46 (76.1%), respectively. cases where hpv dna was negative with the dot blot method but hpv infec ... | 1990 | 2169511 |
| human papilloma virus in erosive oral lichen planus. | several types of human papilloma viruses (hpv) have been associated with benign and malignant squamous cell tumours of mucosal epithelium. to identify hpv in erosive oral lichen planus (olpe), considered as a premalignant lesion, tissues from 20 patients were examined by southern blot hybridization with 32p-labeled hpv dna probes. type 11 was found in 6 of the lesions while hpv types 6, 16 and 18 were not detected in any of the tissues examined. using a type-specific polymerase chain reaction (p ... | 1990 | 2169530 |
| biotin and phosphorus-isotopic labelled dna/rna probes for the detection of human papilloma virus sequences. | in this study, the diagnostic accuracy and practicability of different hybridization techniques for the detection of human papilloma virus (hpv) dna were tested. cervical cell scrapes (n = 67) were analysed for hpv-dnas 6/11 and 16, in order to compare a commercially available in situ dna hybridization-assay with the conventional southern-blot analysis. the in situ dna hybridization-assay gave a sensitivity of 81.5%, a specificity of 97.5% and a diagnostic efficiency of 91.0% for hpv-dnas 6/11. ... | 1990 | 2169684 |
| human papillomavirus type 16 associated with oral squamous carcinoma in a cardiac transplant recipient. | human papillomavirus type 16 (hpv 16) has been associated with a variety of squamous carcinomas, particularly those involving the anogenital tract. the authors report the development of an oropharyngeal carcinoma in a 43-year-old man approximately 20 months after cardiac transplantation while he was on a maintenance regimen of cyclosporine a and prednisone. the carcinoma was resistant to treatment, and he died of complications related to metastatic disease 3 years posttransplantation. molecular ... | 1990 | 2169995 |
| condylomata acuminata in children: frequent association with human papillomaviruses responsible for cutaneous warts. | to identify the papillomavirus types associated with condylomata acuminata in children and to evaluate their mode of transmission, we studied 32 children with anogenital warts. external condylomata were found in 12 of their mothers and in 10 of their fathers. ten mothers, including two without external lesions, had cervical condylomata. blot hybridization studies disclosed a genital human papillomavirus (hpv) in 14 of 27 children (hpv-6 in 12 and hpv-11 in two) and in 8 of 14 patients (hpv-6 in ... | 1990 | 2170467 |
| oral focal epithelial hyperplasia removed with co2 laser. | a case of oral focal epithelial hyperplasia (feh) treated with co2 laser surgery is presented. histological diagnosis is discussed. the association of human papillomavirus (hpv) type 32 with the lesions is demonstrated with dna in situ hybridization technique. laser surgery is suggested as a treatment of choice. | 1990 | 2170540 |
| [study on the localization of human papillomavirus dna in the vulvar disease by means of in situ hybridization]. | we investigated human papillomavirus (hpv) dna of vulvar diseases obtained from 18 cases of condyloma acuminatum, 3 cases of hyperplastic dystrophy without atypia, 4 cases of lichen sclerosus, 4 cases of bowenoid papulosis, one case of bowen disease and 8 cases of squamous cell cancer by in situ hybridization with biotinylated hpv 6, 11, 16 and 18 dna probes. the results of in situ hybridization analysis showed that hpv 6/11 was positive in 94.4% (17/18) of condyloma acuminatum. in 17 cases of h ... | 1990 | 2170544 |
| increased antibody responses to human papillomavirus type 16 l1 protein expressed by recombinant vaccinia virus lacking serine protease inhibitor genes. | the l1 gene of human papillomavirus type 16 (hpv-16) driven by the vaccinia virus major late 4b gene promoter has been inserted into three different sites of the vaccinia virus genome. insertion into the thymidine kinase (tk) gene was achieved by selection of tk- mutants in budr on tk- cells. insertion into two vaccinia virus serine protease inhibitor (serpin) genes was achieved by co-insertion of the escherichia coli xanthine guanine phosphoribosyltransferase gene linked to the vaccinia virus 7 ... | 1990 | 2170578 |
| detection of human papillomavirus-genomic dna in oral epithelial dysplasias, oral smokeless tobacco-associated leukoplakias, and epithelial malignancies. | human papillomavirus (hpv) is an infectious agent that is increasingly associated with mucosal cancers, in particular cancer of the cervix. the present investigation was undertaken in an attempt to determine whether hpv could be easily detected in biopsies of oral tissues, specifically oral squamous cell carcinomas, oral epithelial dysplasias, smokeless tobacco keratoses, verrucous hyperplasia, and verrucous carcinoma. in situ dna hybridization methods were used to isolate specific hpv genomes. ... | 1990 | 2170606 |
| the e6/e7 promoter of human papillomavirus type 16 is activated in the absence of e2 proteins by a sequence-aberrant sp1 distal element. | the e6/e7 promoter of all genital human papillomaviruses is responsible for expression of the viral transforming genes. centered 60 bp upstream of the transcription start, it contains a 20-bp segment with partially overlapping binding sites for the viral e2 proteins and for a cellular factor that was identified by footprint experiments. bandshifts, bandshift competitions, and footprints revealed that protein complexes between nuclear extracts and these sequences have binding properties indisting ... | 1990 | 2170687 |
| monoclonal antibody-mediated neutralization of infectious human papillomavirus type 11. | monoclonal antibodies recognizing human papillomavirus type 11 (hpv-11) were prepared from balb/c mice immunized with intact hpv-11 virions obtained from morphologically transformed human foreskin xenografts grown subrenally in athymic mice. four of five monoclonal antibodies that were reactive by enzyme-linked immunosorbent assay only to intact virions neutralized hpv-11 infectivity in the athymic mouse xenograft system. | 1990 | 2170694 |
| [the effect of vaginal administration of interferon on squamous intraepithelial cervical lesions]. | human papillomavirus vaginal and/or cervical infections were found in 1.98 percent of mostly younger female general populations and in 26.6 percent of cases with abnormal pap smears. antiviral treatment with human leukocyte interferon (hli-alpha) vaginalettes was introduced at the beginning of 1987. in 30 cases a daily dose of 1 x 10(6) iu hli during two 21-day menstrual cycles was applied. in 137 controls without therapy and the treated group a 12-month follow-up was performed. every third mont ... | 1990 | 2170774 |
| [the role of human papillomaviruses among the risk factors for cervical carcinoma]. | in a prospective study, risk factors of the development of cervical carcinoma in 210 diseased and 100 healthy subjects were investigated. special attention was paid to metaplastic changes in the portio vaginalis cervics and to human papilloma virus which are considered responsible for the development of these changes. it has been found that metaplasias are favoured by adolescence, chronic irritative processes in the cervix (pathologic discharge recorded in 51.9% of the diseased and 38% of the he ... | 1990 | 2170775 |
| human papillomavirus associated with poor healing of episiotomy repairs. | a retrospective case-control investigation was conducted for risk factors in 47 parturients whose episiotomies dehisced in the immediate postpartum period. data were extracted from the inpatient, outpatient, and pathology records of each of these 47 patients, as well as from the same records for each of the next two patients who delivered with the same type and degree of episiotomy. no difference between subjects and controls was found for age, parity, or medical history. except for human papill ... | 1990 | 2170886 |
| colposcopic survey of papanicolaou test-negative cases with hyperkeratosis or parakeratosis. | a colposcopic survey was performed in 269 consecutive women with negative cytology showing hyperkeratosis or parakeratosis. a colposcopy-guided biopsy specimen of cervical abnormalities was taken in 88 cases, and human papillomavirus infection (hpv) was detected histologically in 25 cases. no cervical intraepithelial neoplasia (cin) was detected. the detection rate of hpv was not significantly different from that observed in a consecutive series of 1073 papanicolaou test-negative subjects self-r ... | 1990 | 2170888 |
| vulvar carcinomas: search for sequences homologous to human papillomavirus and herpes simplex virus dna. | ten cases of intraepithelial carcinoma, five with bowenoid features and five with early invasion, and ten cases of invasive vulvar carcinoma were examined by in situ hybridization and southern blot analysis using dna probes for human papillomavirus (hpv) types 6, 11, 16, 18 and 31. hpv dna was detected in 90% of the intraepithelial cases and in 10% of the invasive cases. all positive cases showed the presence of dna of hpv type 16. the cases with intraepithelial lesions revealed a strong correla ... | 1990 | 2170970 |
| current concepts in the relationship of human papillomavirus infection to the pathogenesis and classification of precancerous squamous lesions of the uterine cervix. | pathologic and epidemiologic studies performed over the past three decades have provided evidence that the development of squamous cell carcinoma of the cervix is a multistep process involving a precursor preinvasive stage. the results of recent molecular analyses now suggest that the human papillomavirus (hpv) plays a role in this process and is an important but insufficient factor in the development of invasive carcinoma. infection by a variety of hpv types may result in active viral intranucl ... | 1990 | 2171125 |
| endocervical glandular dysplasia, adenocarcinoma in situ, and early invasive (microinvasive) adenocarcinoma of the uterine cervix. | the pathological features of endocervical glandular dysplasia (egd), adenocarcinoma in situ (ais), and early invasive (microinvasive) adenocarcinoma are reviewed. the histology and histochemistry of normal endocervical epithelium is discussed. the association of human papillomavirus (hpv) with the precursor lesions of invasive adenocarcinoma is examined. in the section on ais, particular areas discussed include topography, immunohistochemistry, ploidy, and differential diagnosis. a management pl ... | 1990 | 2171126 |
| analysis of the unoccupied site of an integrated human papillomavirus 16 sequence in a cervical carcinoma. | we have previously cloned and analyzed the structure of a type 16 human papillomavirus (hpv16) integration in a primary cervical carcinoma tissue, m50 (choo et al., j. virol. 62, 1659-1666, 1988). we found that specific nucleotide sequences within the hpv16 genome influenced the genomic organization of the integrated viral genome. using the viral-cellular junctions of the m50 dna as probes, we have now cloned the unoccupied site from a human genomic library. mapping analysis showed that a deleti ... | 1990 | 2171200 |
| the e7 protein of human papillomavirus 8 is a nonphosphorylated protein of 17 kda and can be generated by two different mechanisms. | the e7 protein of human papillomavirus (hpv) 8 shows no in vitro transforming activity, in contrast to e7 of the genital hpvs, but seems to be involved in the control of viral dna replication. to determine whether functional differences between the e7 proteins of hpv16 and hpv8 were reflected in differences in their biochemical properties, we characterized the e7 protein of hpv8 expressed from the late simian virus 40 promoter in cos 7 cells. an e7-specific antiserum was obtained by immunizing r ... | 1990 | 2171214 |
| the use of molecular probes to distinguish new primary tumors from recurrent tumors in gynecologic malignancies. | this is the first report using dna molecular probe technology to distinguish between recurrent tumor and a second primary malignancy in a patient. tumor dna was extracted from squamous cell carcinoma of the cervix at the time of radical hysterectomy. eighteen months later a squamous cell cancer was found in a vaginal apex biopsy from which dna was extracted. tumor dna from both lesions was subjected to restriction enzyme digestion and dna molecular hybridization with human papillomavirus (hpv) p ... | 1990 | 2171321 |
| carbon dioxide laser energy disperses human papillomavirus deoxyribonucleic acid onto treatment fields. | the possibility of dispersing viral deoxyribonucleic acid during carbon dioxide laser treatment of human papillomavirus-containing genital infections has been investigated with a commercially available dot blot hybridization technique. the viral ribonucleic acid probes were specific for groups of human papillomavirus types 6/11, 16/18, and 31/33/35. laser energy was delivered by continuous-wave mode and the plume of smoke was evacuated by a vacuum suction system. samples were taken with dacron s ... | 1990 | 2171340 |
| the use of in situ hybridization to show human papillomavirus deoxyribonucleic acid in metastatic cancer cells within lymph nodes. | southern blot hybridization has been used to identify human papillomavirus types in both primary tumors and lymph node metastases. however, this technique requires fresh-frozen tissue and is incapable of localizing deoxyribonucleic acid sequences to specific cell types in the tumor sample. in contrast, in situ hybridization precisely locates viral sequences within tumor cells while preserving cellular architecture. further, in situ hybridization requires only small samples of formalin-fixed, par ... | 1990 | 2171342 |
| sensitivity and specificity of various morphological features of cervical condylomas. an in situ hybridization study. | fifty-seven cervical biopsy specimens or endocervical curettings showing condyloma, changes suggestive of condyloma, or no changes of condyloma were analyzed for presence of nuclear atypia (nuclear enlargement and irregularity in superficial epithelium), presence of multinucleated cells, and presence of perinuclear cytoplasmic clearing in superficial squamous epithelium. the findings were correlated with results of in situ hybridization with biotin-labeled human papillomavirus dna probes. modera ... | 1990 | 2171450 |
| [the risk factors of invasive carcinoma of the cervix uteri in latin america]. | a study of 759 patients with invasive cervical cancer, 1,430 controls, and 689 sexual partners of the participants who declared that they were monogamous was conducted in colombia, costa rica, mexico, and panama from january 1986 to june 1987, to evaluate the risk factors associated with this neoplasm. the principal risk factors identified were: initiation of sexual relations by the woman at an early age, number of stable sexual partners (relationships of more than three months' duration), numbe ... | 1990 | 2171553 |
| risk factors for invasive carcinoma of the uterine cervix in latin america. | a study of 759 cervical cancer patients, 1,430 controls, and 689 sex partners in four latin american countries has made it possible to assess the influence of multiple factors upon the risk of invasive cervical cancer. the principal risk factors identified were the woman's age at first coitus, the number of her steady sex partners, her number of live births, the presence of dna from human papillomavirus (hpv) types 16 or 18, a history of venereal disease, nonparticipation in early detection prog ... | 1990 | 2171706 |
| the physical state of human papillomavirus 16 dna in cervical carcinoma and cervical intraepithelial neoplasia. | cervical carcinomas and cervical intraepithelial neoplasias (cin) were analyzed for the presence of human papillomavirus (hpv) dna using southern blot hybridization. of the five hpv types examined (hpv types 6, 11, 16, 18, and 33), hpv 16 dna was detected most frequently. in most hpv 16-positive carcinomas examined, hpv 16 dna was present in an integrated state in cellular dna with or without the coexistence of episomal species. in one case, however, only episomal species were detected. among se ... | 1990 | 2171746 |
| differential response of nontumorigenic and tumorigenic human papillomavirus type 16-positive epithelial cells to transforming growth factor beta 1. | the transforming growth factor (tgf) beta s are multifunctional polypeptide growth factors with diverse biological effects, including inhibition of epithelial cell proliferation both in vitro and in vivo. to investigate the possible role of tgf beta 1 in the regulation of papillomavirus infection and papillomavirus-associated transformation, we compared the response to tgf beta 1 of normal keratinocytes, human papillomavirus, type 16 (hpv 16)-positive-immortalized keratinocytes (nontumorigenic), ... | 1990 | 2171761 |
| cytologic and histologic manifestations of human papillomavirus infection of the uterine cervix. | the role of human papillomaviruses (hpv) as possible agents in the genesis of cervical cancer is reviewed. several types of hpv (mainly 6, 11, 16, 18, 31, and 33) have been shown to be associated with precancerous processes and cancer of the uterine cervix. hpv also induces pathognomonic abnormality of squamous cells, known as koilocytosis, that may precede or accompany various manifestations of cancerogenesis including invasive cancer. still, recent studies suggest that hpv infection is quite c ... | 1990 | 2171769 |
| in-situ hybridization for the diagnosis and typing of human papillomavirus. | the human papillomavirus (hpv) has been associated with the production of many skin and mucosal lesions, the development of squamous cell carcinoma of the genital areas, skin and aerodigestive tracts, and possibly adenocarcinoma of the uterine cervix. there are more than 60 known genotypes of hpv. some genotypes have been associated with lesions that have none or minimal chances of malignant transformation; while other genotypes (especially types 16, 18, 31, 35, and 51) have been found in mild, ... | 1990 | 2171813 |
| the presence of human papillomavirus (hpv) in solitary adult laryngeal papillomas demonstrated by in-situ dna hybridization with sulphonated probes. | human papilloma virus (hpv) types 6 and 11 have been repeatedly demonstrated in multiple laryngeal papillomas, and there is little doubt that these lesions are caused by hpv. it has been clearly demonstrated in recent reports that the clinical course of solitary adult onset laryngeal papillomas is entirely different from that of multiple papillomas of juvenile as well as of adult onset. we here report the presence of hpv types 6 and 11 in 19 out of 20 solitary papillomas from 16 patients, while ... | 1990 | 2171818 |
| hairy leucoplakia--a clinical, histopathological and ultrastructural study in 33 patients. | thirty-three cases of hairy leucoplakia (hl) in hiv-positive patients were studied. the most frequent clinical presentation was the development of corrugated plaques on the lateral sides of the tongue without spiculations on the surface. in all biopsies, herpetic-type viral inclusions were found. in 12 out of 14 cases in whom an electromicroscopic study was carried out, only viral particles of the herpes group were found. human papillomavirus (hpv) was not demonstrated, although by means of immu ... | 1990 | 2171819 |
| synthesis, phosphorylation, and nuclear localization of human papillomavirus e7 protein in schizosaccharomyces pombe. | the complete e7 protein-encoding open reading frame of human papillomavirus type 16 (hpv-16) was expressed in the fission yeast schizosaccharomyces pombe, under the control of a cloned yeast promoter. the hpv-16 e7 protein synthesized in s. pombe is a 17-kda phosphoprotein which is recognized by anti-e7 antibodies (raised in rabbits against e7 fusion protein produced in escherichia coli). the mobility during sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of native e7 phosphoprotein sy ... | 1990 | 2172091 |
| rapid dysplastic transformation of human genital cells by human papillomavirus type 18. | this study delineates differences in biologic activity between human papillomavirus (hpv) types 16 and 18. human cervical and foreskin epithelial cells were cultured and transfected with recombinant hpv-16 and -18 dna, resulting in immortalized cell lines. normal epithelial cells as well as hpv-16 and -18 immortalized cells of both early passages (less than 40 population doublings) and late passages (greater than 180 population doublings) were transplanted in athymic mice. normal squamous cells ... | 1990 | 2172117 |
| investigation of ovarian neoplasia of low malignant potential for human papillomavirus. | recent in situ hybridization studies have suggested the presence of human papillomavirus type 6 (hpv-6) dna in ovarian cancer cells. an association between hpv and ovarian neoplasia of low malignant potential (lmp) has not been previously identified. paraffin-embedded tissue blocks from 24 patients with lmp ovarian tumors were screened for human papillomavirus dna. the patients ranged in age from 18 to 73 years. corresponding microscopic slides from each tissue block were reviewed to confirm the ... | 1990 | 2172118 |
| human cervical cells immortalized in vitro with oncogenic human papillomavirus dna differentiate dysplastically in vivo. | human papillomavirus (hpv) types 16 and 18 are associated with cervical dysplasia and carcinoma. in vitro integration of hpv-16 or hpv-18 dna into cultured human cervical cells results in their immortalization. in this study, in vivo differentiation of human cervical cells immortalized with recombinant hpv-16 or hpv-18 dna was studied by implanting confluent monolayers of hpv-16 and hpv-18 dna immortalized cell lines under dorsal skin flaps of immunodeficient mice. grafts of a human cervical can ... | 1990 | 2172120 |
| detection of human papillomavirus types 6/11, 16 and 18 in exfoliated cells from the uterine cervices of japanese women with and without lesions. | human papillomavirus (hpv) infection of the uterine cervices of japanese women with and without lesions was identified by the filter in situ hybridization method. exfoliated cervical cells from 23 cervical papillary condylomas, 70 cervical intraepithelial neoplasia (cin) grade i/ii, 26 cin iii, 31 invasive cervical cancers and 666 cervices without evidence of disease (including 53 pregnant women) were tested for the presence of hpv types 6/11, 16 and 18. the positive rates for the detection of h ... | 1990 | 2172197 |
| use of affiprobe hpv test kit for detection of human papillomavirus dna in genital scrapes. | the presence of human papillomavirus (hpv) dna in cervical and vaginal scrapes was analyzed by the affiprobe hpv test kit (orion corp., orion pharmaceutica, helsinki, finland), which is a 1-day solution hybridization test for hpv type 6/11, 16, or 18. the affiprobe test was compared with a commercially available dot blot test (virapap and viratype tests; life technologies inc., gaithersburg, md.). the study group consisted of 178 patients seen in a gynecological outpatient clinic. altogether, 64 ... | 1990 | 2172298 |
| sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridisation. | the sensitivity of digoxigenin and biotin labelled dna probes for the detection of human papillomavirus (hpv) by dot blotting and in situ hybridisation was compared in tissues from cervical, laryngeal, and anogenital neoplasia. probes were either labelled with digoxigenin by the random primer technique and detected with anti-digoxigenin antibody, or labelled with biotin by nick translation and detected with streptavidin, both methods having a common final visualisation procedure using alkaline p ... | 1990 | 2172319 |
| cutaneous horn of the penis: its association with squamous cell carcinoma and hpv-16 infection. | cutaneous horns of the penis are rare. including this case, only 19 cases have been reported in the english-language literature. in 37% of the reported cases a malignant tumor was found beneath the cutaneous horn. our case is remarkable because a stage i squamous cell carcinoma developed on the shaft of the penis of a neonatally circumcised man. human genital carcinoma resulting from a multifactorial process in which "promoting" papillomavirus is an integral element is being increasingly reporte ... | 1990 | 2172337 |
| widespread flat warts associated with human papillomavirus type 5: a cutaneous manifestation of human immunodeficiency virus infection. | numerous flat and tinea versicolor-like warts developed on the face, trunk, and upper extremities of a 10-year-old boy with human immunodeficiency virus infection. nucleic acid analysis of involved skin revealed human papillomavirus type 5, which has sometimes been associated with epidermodysplasia verruciformis. this human papillomavirus type has also been described in patients with common variable immunodeficiency and dyskeratosis congenita and in renal allograft recipients. human immunodefici ... | 1990 | 2172338 |
| the diagnosis and treatment of genital human papillomavirus lesions. | a gynecologist in solo practice reviewed three years of experience in the diagnosis and treatment of human papillomavirus (hpv). infected women were asked to have their sexual partners diagnosed and treated. one hundred nineteen women and 57 men were examined. this article describes common hpv lesion types and examination methods for both sexes. magnification and use of dilute acetic acid are emphasized. the most common treatments and the pros and cons of various therapeutic methods are discusse ... | 1990 | 2172365 |
| use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. | the polymerase chain reaction was adapted to the amplification of a herpes simplex virus (hsv) dna sequence, common to hsv types 1 and 2 (hsv-1, hsv-2). the amplified product was detectable by ethidium-bromide staining or southern hybridization of gels and by dot hybridization. the hsv polymerase chain reaction detected hsv dna in samples obtained from eight patients with genital lesions from which hsv-2 was isolated in tissue culture and from four patients with labial lesions from which hsv-1 w ... | 1990 | 2172392 |
| [the possibility of viral etiology in cervical carcinogenesis]. | oncogenic potential of herpes simplex virus type 2 (hsv-2) and human papillomavirus (hpv) which are both associated with occurrence of cervical cancer and the mechanism of oncogenesis by these viruses were investigated by transformation experiments in vitro. the results were obtained as follows. 1) hsv-2 induced neoplastic transformation of normal diploid cells is a multistep process. cervical cancer associated antigen ag-4 is encoded within the specific region of hsv-2 dna which converts immort ... | 1990 | 2172417 |
| [studies on pathogenesis of cervical carcinoma based on the analysis of growth and differentiation mechanism of cervical epithelium]. | recently cervical cancer is defined as a sexually-transmitted disease, and human papillomavirus (hpv) has been focused as one of its etiologic agents. it is known that cervical cancer is extraordinarily rare in non-human mammals that have the estrous cycle. in contrast, cervical cancer is frequent in human beings which have lost the estrous cycle, and subsequently evolved a sexual behavior irrespective of the menstrual phase. therefore, upon the hypothesis that the estrous cycle is a period prot ... | 1990 | 2172418 |
| [molecular biologic study on the carcinogenesis of hpv in uterine cervical cancer and related lesions--analysis of hpv types 16, 18 e6/e7 gene mrna]. | recent molecular biologic studies have shown that some type of human papillomavirus (hpv) is closely associated with uterine cervical cancer. in order to investigate the relationship between hpv dna and cervical cancer, we studied the carcinogenesis of hpv in cervical cancer and related lesions by dot blot hybridization (d.b.h.), southern blot hybridization (s.b.h.), in situ hybridization (i.s.h.) and polymerase chain reaction (p.c.r.). we also followed some hpv dna, mrna positive or negative ca ... | 1990 | 2172419 |
| [virological studies on generation of cervical carcinomas]. | 1. detection of hpv in cytologically normal cervices: we have detected by filter in situ hybridization human papillomavirus (hpv) types 6/11, 16 and 18 dna sequences in 6(0.9%), 12(1.8%) and 4(0.6%), respectively, out of 666 swab specimens from normal cervices (mean age; 49.1 years). the positive rate for hpv 16 and hpv 18 was significantly lower compared with 27.1%(26/96) of cin and 48.4%(15/31) of cervical cancers. hpv dna occurred more often in women under 50 years old than in those 50 years ... | 1990 | 2172420 |
| human papillomavirus type 8 contains cis-active positive and negative transcriptional control sequences. | human papillomavirus type 8 (hpv-8) is one aetiological agent of macular and flat wart-like lesions in patients with epidermodysplasia verruciformis and appears to be closely linked to skin carcinogenesis. a 1.2 kb region of the genome, which was previously shown to contain a viral e2-dependent enhancer, was progressively shortened from both ends with bal 31. the resulting fragments were tested for their ability to stimulate chloramphenicol acetyltransferase (cat) expression from the simian viru ... | 1990 | 2172459 |
| effect of perilesional injection of alpha-interferon on cervical intraepithelial neoplasia and associated human papillomavirus infection. | in this controlled prospective study, 14 patients with cervical intraepithelial neoplasia (cin) were observed for one month to exclude spontaneous regression, and then seven patients were treated twice-weekly with perilesional injections of alpha-interferon. both groups were monitored colposcopically, and advised to use the contraceptive sheath. cervical smears and biopsies for histology and viral studies were taken before and after the study. there was an improvement of six out of seven of the ... | 1990 | 2172535 |
| type-specific human papillomavirus detection in formalin-fixed, paraffin-embedded tissue sections using nonradioactive deoxyribonucleic acid probes. | dna probes directly conjugated to horseradish peroxidase have been used successfully to detect human papillomavirus (hpv) types 6/11, 16, and 18 in formalin-fixed, paraffin-embedded tissue sections. by using silver enhancement of a heavy metal-modified diaminobenzidine precipitate, the sensitivity of human papillomavirus detection was significantly increased without compromising specificity. in studies comparing the specificity of the horseradish peroxidase-labeled probe/silver enhancement syste ... | 1990 | 2172649 |
| comparative analysis of human papillomavirus detection by polymerase chain reaction and virapap/viratype kits. | cervical samples from 270 women referred by area physicians were analyzed for the presence of human papillomavirus (hpv) types 6, 11, 16, and 18 by the polymerase chain reaction (pcr) followed by southern hybridization. samples from 154 patients were concurrently analyzed by a commercial filter hybridization technique (virapap and viratype kits, life technologies, bethesda research labs, gaithersburg, md). the sensitivity of the southern blot procedure combined with pcr was significantly higher ... | 1990 | 2173396 |
| comparison of southern transfer hybridization and dot filter hybridization for detection of cervical human papillomavirus infection with types 6, 11, 16, 18, 31, 33, and 35. | a commercial dot filter hybridization kit (virapap kit) was compared with southern transfer hybridization for the detection of seven types of human papillomavirus (hpv) in cervical specimens from 450 consecutive females attending a sexually transmitted diseases clinic. in comparison with southern transfer hybridization, performed with the same probes used in the dot filter kit, the sensitivity, specificity, and positive and negative predictive values of dot filter hybridization were 90%, 94%, 74 ... | 1990 | 2173397 |
| survey of histologic specimens of human cancer for human papillomavirus types 6/11/16/18 by filter in situ hybridization. | histologic specimens (317) of genital and nongenital cancers and normal tissue were analyzed for the presence of the dna of human papillomavirus (hpv) 6, 11, 16, and 18 by filter in situ hybridization performed on paraffin-embedded, formalin-fixed tissue (histofish). hpv dna was found in 73 of 172 (42%) anogenital lesions and 17 of 116 (15%) nonanogenital carcinomas. no hpv dna was found in normal mouse skin (five samples), human autopsy liver (two samples), or kidney (eight samples), or in carc ... | 1990 | 2173398 |
| casein kinase ii activities related to hyperphosphorylation of human papillomavirus type 16-e7 oncoprotein in epidermal keratinocytes. | the present study describes hyper-phosphorylation of e7-oncoprotein of human papillomavirus (hpv) type 16 in epidermal keratinocytes. we found that highly phosphorylated e7-oncoprotein was present in epidermal keratinocytes but little in fibroblasts. the e7 oncoprotein contains serine residues (ser-ser-glu-glu-glu) capable of being phosphorylated by casein kinase ii (ck ii). extracts from various cell lines including human origins transformed by hpv 16 were examined for the casein kinase activit ... | 1990 | 2173586 |
| severe intractable common warts associated with human papillomavirus 2, 3 and 20. | a 26-year-old male had had numerous common warts on the feet and hands since childhood. they had repeatedly undergone etretinate cryosurgery, always responding well to it. however, the warts reappeared. human papillomavirus (hpv) 2, 3 and 20 were detected in the warts using southern's blot hybridization technique with hpv dna. histologically, the warts showed swollen, clear keratinocytes with vacuolated degeneration not found in common warts. the findings are presented in this paper. | 1990 | 2173668 |
| time trends in the prevalence of human papillomavirus infections in archival papanicolaou smears: analysis by cytology, dna hybridization, and polymerase chain reaction. | a retrospective study was undertaken to determine the prevalence of human papillomavirus (hpv) infections in routine papanicolaou (pap) smears collected by general practitioners from western australian women in each of the years 1972, 1982, and 1987. hpv infection was detected by cytology, dot-blot hybridization, or polymerase chain reaction (pcr). it was found that the prevalence of hpv infection remained unchanged over the 15 year study period, was independent of age, and was associated with n ... | 1990 | 2173733 |
| incidence of antibodies to human papillomavirus type 1 in patients with cutaneous and mucosal papillomas. | a study of antibodies to human papillomavirus (hpv) 1 is reported in a series of sera from 126 patients with papilloma lesions of different clinical types (verruca plantaris, vulgaris, plana, condyloma acuminata, laryngeal papilloma) using a sensitive solid-phase enzyme-linked immunosorbent assay (elisa). our findings showed a significant prevalence of specific hpv 1 igg antibodies in patients with plantar warts (65%). this incidence was considerably lower in patients with other papilloma lesion ... | 1990 | 2173734 |
| automated polymerase chain reaction for papillomavirus screening of cervicovaginal lavages: comparison with dot-blot hybridization in a sexually transmitted diseases clinic population. | the aim of the present study was to compare the recently developed polymerase chain reaction (pcr) technique with conventional dot-blot dna hybridization for human papillomavirus (hpv) detection. cells were collected by cervicovaginal lavage from a study group of 109 women attending a sexually transmitted diseases clinic. using a machine that we developed for alternation of temperature cycles, hpv was detected in 51% of patients by pcr. by dot-blot hybridization, 44% of the patients were positiv ... | 1990 | 2173735 |
| chemical synthesis of human papillomavirus type 16 e7 oncoprotein: autonomous protein domains for induction of cellular dna synthesis and for trans activation. | the human papillomavirus type 16 e7 protein belongs to a family of nuclear oncoproteins that share amino acid sequences and functional homology. to localize biochemical activities associated with e7, we chemically synthesized the full-length 98-amino-acid polypeptide and several deletion mutant peptides. we show that the e7 polypeptide is biologically active and possesses at least two functional domains; the first induces cellular dna synthesis in quiescent rodent cells, and the second trans act ... | 1990 | 2173783 |
| production of human papillomavirus type 16 virions in a keratinocyte cell line. | human papillomavirus type 16 (hpv-16) is strongly associated with carcinoma of the cervix, but the complete life cycle of the virus cannot be studied because no experimental system is available in which hpv-16 progeny are produced, and there is currently no source of hpv-16 virus particles. most cell lines that harbor hpv-16 dna contain the viral genome as integrated or concatenated dna in which open reading frames are disrupted or deleted, but a human cervical keratinocyte cell line has been de ... | 1990 | 2173795 |
| [asymptomatic and subclinical human papillomavirus infections of the genital organs and anus in males]. | 1990 | 2173853 | |
| verrucous carcinoma of the anus containing human papillomavirus type 16 dna detected by in situ hybridisation: a case report. | verrucous carcinoma of the anus is a distinct and rare variant of well-differentiated squamous cell carcinoma. a case of anal verrucous carcinoma in a 35 year old man was studied by light microscopy and in situ dna hybridization. human papillomavirus (hpv) type 16 dna sequences were demonstrated in the tumour cells, as well as in cells showing koilocytotic and dyskeratotic changes. the possible viral (hpv) aetiology of verrucous carcinoma is considered with a review of the previous literature. | 1990 | 2174020 |
| cytologic correlates of cervical papillomavirus infection. | a fundamental question in papanicolaou smear screening is the specificity of cytologic criteria for the recognition of genital human papillomavirus (hpv) infection. to address this problem, we conducted a two-phase study of routinely screened women to determine the efficiency with which cytologic findings identified the presence of hpv dna, focusing on the criteria for identifying smears as "atypical." in phase 1, 25 of 290 (8.6%) smears were designated atypical, but only 3 (12%) of the samples ... | 1990 | 2174025 |
| detection of human papillomavirus dna in cervical lesions by in situ hybridization using biotinylated dna probes. | in situ hybridization (ish) for human papillomavirus (hpv)-6, -11, -16, -18, and -31 dna was performed on 615 formalin-fixed paraffin-embedded cervical biopsies using biotinylated dna probes. results were obtained from 584 samples with 266 (45.5%) containing hpv-dna sequences. ninety percent of condyloma acuminatum specimens were positive for hpv-dna with 18 of 19 positive cases containing hpv-6 or -11 dna. the detection rate of hpv in cervical intraepithelial neoplasia (cin) lesions was 50.6% ( ... | 1990 | 2174026 |
| human papillomavirus infections of the genital tract. | infection of the genital tract by hpv is a sexually transmitted disease of increasing prevalence. the association of hpv infection with genital tract malignancies is of great concern, and further studies are needed to clarify this association. few investigators believe at this time that proof of a direct causative role exists for hpv in these cancers, but indirect evidence of such a role is abundant. there are many clinical forms of hpv infection of the genital tract, and few clinicians can easi ... | 1990 | 2174093 |
| maintenance of cellular proliferation by adenovirus early region 1a in fibroblasts conditionally immortalized by using simian virus 40 large t antigen requires conserved region 1. | various mutants of adenovirus e1a were assayed for their ability to complement the growth defect at the nonpermissive temperature for the cell line tsa14 which was isolated by immortalizing rat embryo fibroblasts with the thermolabile large t antigen of tsa58. this cell line grows indefinitely at the permissive temperature but undergoes rapid growth arrest upon shift up to the nonpermissive temperature. since this growth arrest can be overcome by introduction of wild-type simian virus 40 large t ... | 1990 | 2174113 |
| an update on human papillomavirus infection of the cervix. | human papillomavirus infection (hpv) of the cervix has been an area of intense research during the 1980s. knowledge has expanded rapidly and new advances have often challenged previous ideas. this review is designed to help the general practitioner remain up-to-date with recent research findings and to assist in the management of women with hpv infection. | 1990 | 2174238 |
| genetic strategies of tumor suppression. | the evaluation of the cancer cell is a complex multigene process. tumor suppressor genes that are lost or inactivated, as well as genes that are overexpressed, play key roles in tumor progression. the identification of overexpressed genes has been expedited by the presence of transforming genes in some animal retroviruses. however, tumor suppressor genes have been difficult to identify and isolate because of their loss or inactivation during tumorigenesis. by a variety of methods, summarized in ... | 1990 | 2174661 |
| occurrence of iga and igg antibodies to select peptides representing human papillomavirus type 16 among cervical cancer cases and controls. | we used an enzyme-linked immunosorbent assay to test sera from 186 cases of invasive cervical cancer and 172 age-matched controls for igg and iga antibodies to an human papillomavirus 16 e7 peptide and to peptide 245, representing an epitope in e2. cases had significantly higher mean absorbance values than controls for both immunoglobulin isotypes to e7 and elevated mean values for igg to peptide 245. since absorbances were not normally distributed we analyzed cervical cancer risk for seropositi ... | 1990 | 2174733 |
| epstein-barr virus associated oesophageal ulcers in aids. | epstein-barr virus (ebv) associated ulceration has not previously been included in the differential diagnosis of oesophageal ulcers in aids. we report five cases of oesophageal ulceration in homosexual men with advanced human immunodeficiency virus infection in whom this was considered to be the most likely cause. dna in situ hybridisation studies showed ebv in biopsy material from three of four patients with oesophageal ulcers and in none of three controls. of other viruses studied, only human ... | 1990 | 2174817 |
| occurrence of human papillomavirus type 16 dna in cutaneous squamous and basal cell neoplasms. | sixty-eight cutaneous squamous cell neoplasms (in situ and invasive) and 26 basal cell carcinomas from 89 patients were analyzed for dna sequences homologous to the human papillomavirus (hpv) types found predominantly in the genital tract. thirty-six (53%) of the squamous cell neoplasms contained hpv dna as detected by filter or in situ hybridization analysis. the frequency of detection of hpv dna was dependent on the site of the lesion. of 40 genital squamous cell neoplasms (penile, vulvar, and ... | 1990 | 2174930 |
| multiple interactions between cellular factors and the non-coding region of human papillomavirus type 16. | the interaction between cellular factors and the non-coding region (ncr) of human papillomavirus type 16 (hpv-16) has been studied using different approaches; dnase i hypersensitive sites (hss) analysis of hpv-16 chromatin in siha cells, footprinting (fp) of the ncr in vitro with nuclear extracts from siha cells and band shift analysis of synthetic oligonucleotides corresponding to the hss and footprint sites with nuclear extracts from a variety of cells. these analyses have shown that cellular ... | 1990 | 2174960 |
| synthetic peptides derived from e7 region of human papillomavirus type 16 used as antigens in elisa. | nine overlapping peptides (20 amino acid) covering the entire sequence of early antigen e7 of human papillomavirus type 16 (hpv-16) were synthesized and tested as antigens with human sera in elisa. five of these peptides (no. 1 to 5 counting from the n terminus of the e7 protein) reacted with a pool of sera from hpv-16-infected individuals (as determined by molecular hybridization with their biopsy specimens); one (no. 5) was also reactive with pools of hpv-18- and hpv-6- or 11-infected individu ... | 1990 | 2174961 |
| expression of the full-length products of the human papillomavirus type 6b (hpv-6b) and hpv-11 l2 open reading frames by recombinant baculovirus, and antigenic comparisons with hpv-11 whole virus particles. | the l2 open reading frames (orfs) of human papillomavirus (hpv) types 6b and 11 were expressed as full-length non-fusion proteins in spodoptera frugiperda (sf-9) cells using recombinant baculovirus. both proteins were detected on western blots as immunoreactive bands which migrated with apparent mrs of 76k and 78k, respectively, and contained both cross-reactive and type-specific epitopes, as determined by polyclonal antisera directed against defined subregions of the hpv-6b and hpv-11 l2 orfs. ... | 1990 | 2174962 |
| [transforming genes of human papillomavirus]. | 1990 | 2174990 | |
| human papillomavirus infection in sexually active adolescent females: prevalence and risk factors. | we examined the prevalence of human papillomavirus (hpv) infection, and associated risk factors for infection with hpv types 6, 11, 16, 18, 31, 33, and 35, in 661 sexually active adolescent females attending family planning clinics. fifteen percent were positive for hpv dna by rna-dna dot-blot hybridization. more than 60% of the hpv-positive subjects harbored at least one of the following cancer-related hpv types: 16, 18, 31, 33, or 35. those with hpv had a mean range of four to 10 lifetime sexu ... | 1990 | 2175024 |
| routine colposcopic survey of patients with squamous atypia. a method for identifying cases with false-negative smears. | routine colposcopy was performed on 376 women with cervical squamous atypia (originally reported as "inflammatory atypia"). colposcopy showed no abnormalities in 240 cases and a lesion in 136 cases; the latter were sampled by colposcopy-guided biopsy. the biopsy samples showed evidence of human papillomavirus (hpv) infection and/or grade i cervical intraepithelial neoplasia (cin i) in 42 cases (11.1%), cin ii in 4 cases (1.1%) and cin iii in 5 cases (1.3%); the other 85 biopsied cases were histo ... | 1990 | 2175135 |
| "nonclassic" cytologic signs of cervical condyloma. a case-control study. | the association between "nonclassic" cytologic signs of condyloma and human papillomavirus (hpv) infection in women with negative papanicolaou smears was analyzed via a case-control study. the cytologic signs considered were mild koilocytosis, mild dyskeratosis, binucleation or multinucleation, cleared cytoplasm and nuclear hyperchromatism. the papanicolaou smears of 166 cases that showed colposcopic and histologic evidence of hpv infection (but whose smears lacked the classic cytologic signs of ... | 1990 | 2175136 |
| vestibular papillae of the vulva. lack of evidence for human papillomavirus etiology. | vestibular papillae of the vulva are usually considered as anatomical variants of the vestibular mucosa. clinically, however, they are quite often interpreted as condylomata acuminata and recent studies have suggested that they could be related to human papillomavirus infection. this prompted us to search for human papillomavirus dna using the southern blot hybridization technique, by analyzing biopsy specimens taken from 29 patients who presented with papillae of the vestibular mucosa. human pa ... | 1990 | 2175164 |
| 'cystic papilloma' in humans? demonstration of human papillomavirus in plantar epidermoid cysts. | fourteen cases of plantar epidermoid cyst were studied immunohistochemically and five of them were also studied electron microscopically for the presence of human papillomavirus. in eleven cases, the test results were positive for papillomavirus antigens, and in five of them, papillomaviruslike particles were observed by electron microscopy. histological examination showed most of the cysts to contain intracytoplasmic eosinophilic bodies in cells of the cyst wall as well as the vacuolar structur ... | 1990 | 2175165 |
| human papillomavirus associated with keratoacanthomas in australian patients. | 1990 | 2175166 | |
| [detection of human papillomavirus dna in the normal genitalia of men]. | the study of human papillomavirus (hpv) in the genitalia of all normal males is difficult, therefore cases of chronic prostatitis, a common disease treated in urological clinics, were chosen to identify hpv dna by using vira type (life technologies inc.). smears of glans and sulcus coronalius of 177 subjects, showed a hpv positive rate of 3.4%, while 86 cases of those cases were negative for hpv in urethral smears. the lack of clinical findings suggests that hpv is an asymptomatic infection. in ... | 1990 | 2175330 |
| the e6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. | the e6 protein encoded by the oncogenic human papillomavirus types 16 and 18 is one of two viral products expressed in hpv-associated cancers. e6 is an oncoprotein which cooperates with e7 to immortalize primary human keratinocytes. insight into the mechanism by which e6 functions in oncogenesis is provided by the observation that the e6 protein encoded by hpv-16 and hpv-18 can complex the wild-type p53 protein in vitro. wild-type p53 gene has tumor suppressor properties, and is a target for sev ... | 1990 | 2175676 |
| cervical intraepithelial neoplasia iii in an adolescent with bowenoid papulosis. | bowenoid papulosis (bp) is a cutaneous condition of the external genitalia seen primarily in young adults. evidence supports an etiologic role of human papillomavirus (hpv), particularly type 16. hpv-16 is also associated with an increased risk for cervical intraepithelial neoplasia (cin) and invasive carcinoma. a 17-year-old female, referred to the adolescent dysplasia clinic with a diagnosis of condyloma acuminatum, was presented. she had multiple smooth, shiny, brown 3- to 4-mm papules on the ... | 1990 | 2175743 |
| fast multiplex polymerase chain reaction on boiled clinical samples for rapid viral diagnosis. | an assessment of optimal conditions for rapid simultaneous amplification of multiple human papillomavirus (hpv) sequences has been made using thermus aquaticus dna polymerase. all variables of practical value were studied by amplifying known target-sequences from ten-fold dilutions of well characterized cell lines. in our hands, fast multiplex pcr (fm-pcr), the technique of running multiple pcr reactions simultaneously with minimum incubation time at each temperature, was highly sensitive (ampli ... | 1990 | 2175751 |
| human papillomavirus in oesophageal squamous cell carcinoma. | thirty seven cases of oesophageal squamous cell carcinoma were studied by applying dna slot blot analysis and in situ hybridisation using type specific probes for hpv 6, 11, 16 and 18. cases of condyloma accuminata, cervical carcinoma, and laryngeal papilloma were used as controls. blocks including areas of invasive carcinoma, intraepithelial neoplasia, and normal epithelium were studied in each case. no hpv genome was detectable in any of the oesophageal cases. it is concluded that these types ... | 1990 | 2175754 |