Publications

how does the host-specialized aphid deal with food deficiency?	aphis gossypii glover shows obvious host specialization, with cucurbit- and cotton-specialized biotypes or host races in many regions. because its annual natal host crops senesce earlier the cucurbit-specialized biotype may suffer food deficiency. the method this biotype uses to overcome this challenge is still poorly understood. in order to understand the potential of the cucurbit-specialized biotype aphids in host shift and usage, the performance of this biotype on cotton (gossypium hirsutum), ...	2014	24526656
construction of a complete set of alien chromosome addition lines from gossypium australe in gossypium hirsutum: morphological, cytological, and genotypic characterization.	we report the first complete set of alien addition lines of g. hirsutum . the characterized lines can be used to introduce valuable traits from g. australe into cultivated cotton. gossypium australe is a diploid wild cotton species (2n = 26, gg) native to australia that possesses valuable characteristics unavailable in the cultivated cotton gene pool, such as delayed pigment gland morphogenesis in the seed and resistances to pests and diseases. however, it is very difficult to directly transfer ...	2014	24553965
molecular characterization of cotton c-repeat/dehydration-responsive element binding factor genes that are involved in response to cold stress.	low temperature, drought and salinity are major abiotic stresses that influence survival, productivity and geographical distribution of many important crops across the globe. the c-repeat/dehydration-responsive element binding transcription factors (cbf/dreb) are important proteins involved in response to abiotic stresses in plants. in this study, twenty-one cbf genes were identified in cotton (gossypium hirsutum) by bioinformatic approach. the twenty-one cbf genes (named as ghcbf1--ghcbf21) wer ...	2014	24566693
the effects of fruiting positions on cellulose synthesis and sucrose metabolism during cotton (gossypium hirsutum l.) fiber development.	cotton (gossypium hirsutum l.) boll positions on a fruiting branch vary in their contribution to yield and fiber quality. fiber properties are dependent on deposition of cellulose in the fiber cell wall, but information about the enzymatic differences in sucrose metabolism between these fruiting positions is lacking. therefore, two cotton cultivars with different sensitivities to low temperature were tested in 2010 and 2011 to quantify the effect of fruit positions (fps) on fiber quality in rela ...	2014	24586807
changes during leaf expansion of φpsii temperature optima in gossypium hirsutum are associated with the degree of fatty acid lipid saturation.	in this project, we hypothesize that cotton (gossypium hirsutum) leaf temperature and the responses of leaf photosynthesis to temperature will change as the leaves expand and that differences between young and mature leaves will be associated with the proportion of saturated fatty acids in thylakoid and other membrane lipids. to that end, we studied main stem leaves obtained from plants growing in a temperature controlled greenhouse and at different times in the field season. we found that young ...	2014	24594393
the li2 mutation results in reduced subgenome expression bias in elongating fibers of allotetraploid cotton (gossypium hirsutum l.).	next generation sequencing (rna-seq) technology was used to evaluate the effects of the ligon lintless-2 (li2) short fiber mutation on transcriptomes of both subgenomes of allotetraploid cotton (gossypium hirsutum l.) as compared to its near-isogenic wild type. sequencing was performed on 4 libraries from developing fibers of li2 mutant and wild type near-isogenic lines at the peak of elongation followed by mapping and polycat categorization of rna-seq data to the reference d5 genome (g. raimond ...	2014	24598808
virus-induced gene silencing using a modified betasatellite: a potential candidate for functional genomics of crops.	betasatellites are geminivirus-associated single-stranded dna molecules that play an important role in symptom modulation. a vigs vector was developed by modifying cotton leaf curl multan betasatellite (clcumb). clcumb dna was modified by replacing the βc1 gene with a multiple cloning site. the silencing ability of the modified clcumb was investigated by cloning a fragment of a host gene (su) or a reporter transgene (uida) into the modified clcumb and co-agroinoculation with cotton leaf curl mul ...	2014	24610555
genetic dissection of upland cotton (gossypium hirsutum) cultivars developed in hubei province by mapped ssrs.	the genetic diversity of 51 upland cotton cultivars with different parental origins and breeding periods that were developed in hubei province was studied on the basis of 237 mapped simple sequence repeat markers covering the cotton genome. a total of 108 polymorphic primer pairs amplified 196 loci; the polymorphism information content range was 0.04 to 0.83, with an average of 0.46. a model-based clustering analysis (structure) of the genomic data identified 3 clear subpopulations, and the resu ...	2014	24615042
upland cotton gene ghfpf1 confers promotion of flowering time and shade-avoidance responses in arabidopsis thaliana.	extensive studies on floral transition in model species have revealed a network of regulatory interactions between proteins that transduce and integrate developmental and environmental signals to promote or inhibit the transition to flowering. previous studies indicated flowering promoting factor 1 (fpf1) gene was involved in the promotion of flowering, but the molecular mechanism was still unclear. here, fpf1 homologous sequences were screened from diploid gossypium raimondii l. (d-genome, n = ...	2014	24626476
pyramiding b genes in cotton achieves broader but not always higher resistance to bacterial blight.	near-isogenic lines of upland cotton (gossypium hirsutum) carrying single, race-specific genes b4, bin, and b7 for resistance to bacterial blight were used to develop a pyramid of lines with all possible combinations of two and three genes to learn whether the pyramid could achieve broad and high resistance approaching that of l. a. brinkerhoff's exceptional line im216. isogenic strains of xanthomonas axonopodis pv. malvacearum carrying single avirulence (avr) genes were used to identify plants ...	2014	24655289
a peptide hormone gene, ghpsk promotes fibre elongation and contributes to longer and finer cotton fibre.	cotton fibres, the single-celled trichomes derived from the ovule epidermis, provide the most important natural material for the global textile industry. a number of studies have demonstrated that regulating endogenous hormone levels through transgenic approaches can improve cotton fibre qualities. phytosulfokine-α (psk-α) is a novel peptide hormone in plants that is involved in regulating cell proliferation and elongation. however, its potential applications in crop genetic improvement have not ...	2014	24666593
dynamics of soil diazotrophic community structure, diversity, and functioning during the cropping period of cotton (gossypium hirsutum).	the soil sampled at different growth stages along the cropping period of cotton were analyzed using various molecular tools: restriction fragment length polymorphism (rflp), terminal restriction length polymorphism (t-rflp), and cloning-sequencing. the cluster analysis of the diazotrophic community structure of early sampled soil (0, 15, and 30 days) was found to be more closely related to each other than the later sampled one. phylogenetic and diversity analysis of sequences obtained from the f ...	2015	24677076
seed-specific expression of a lysine-rich protein gene, ghlrp, from cotton significantly increases the lysine content in maize seeds.	maize seed storage proteins are a major source of human and livestock consumption. however, these proteins have poor nutritional value, because they are deficient in lysine and tryptophan. much research has been done to elevate the lysine content by reducing zein content or regulating the activities of key enzymes in lysine metabolism. using the naturally lysine-rich protein genes, sb401 and sbglr, from potato, we previously increased the lysine and protein contents of maize seeds. here, we exam ...	2014	24681583
[effects of fertilization on cotton growth and nitrogen use efficiency under salinity stress].	cotton (gossypium hirsutum) was raised at different salinity levels (0, 0.15% and 0.30%) by irrigating with fresh- or sea-water. the effects of fertilization (n, nk, np and npk) on plant growth, nitrogen (n) uptake and n use efficiency were studied. the results showed that salinity and fertilization both affected the biomass, agronomic n use efficiency, n bioavailability and nitrogen accumulation of plants, and significant interaction was observed between the two factors. fertilization could imp ...	2013	24697064
quantitative phosphoproteomics analysis of nitric oxide-responsive phosphoproteins in cotton leaf.	knowledge of phosphorylation events and their regulation is crucial to understanding the functional biology of plant proteins, but very little is currently known about nitric oxide-responsive phosphorylation in plants. here, we report the first large-scale, quantitative phosphoproteome analysis of cotton (gossypium hirsutum) treated with sodium nitroprusside (nitric oxide donor) by utilizing the isobaric tag for relative and absolute quantitation (itraq) method. a total of 1315 unique phosphopep ...	2014	24714030
ghpsy, a phytoene synthase gene, is related to the red plant phenotype in upland cotton (gossypium hirsutum l.).	carotenoids are important accessory pigments in plants that are essential for photosynthesis. phytoene synthase (psy), a rate-controlling enzyme in the carotenoid biosynthesis pathway, has been widely characterized in rice, maize, and sorghum, but at present there are no reports describing this enzyme in cotton. in this study, ghpsy was identified as a candidate gene for the red plant phenotype via a combined strategy using: (1) molecular marker data for loci closely linked to r1; (2) the whole- ...	2014	24718783
characterization of developmental- and stress-mediated expression of cinnamoyl-coa reductase in kenaf (hibiscus cannabinus l.).	cinnamoyl-coa reductase (ccr) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis. we have cloned a full length coding sequence of ccr from kenaf (hibiscus cannabinus l.), which contains a 1,020-bp open reading frame (orf), encoding 339 amino acids of 37.37 kda, with an isoelectric point (pi) of 6.27 (jx524276, hcccr2). blast result found that it has high homology with other plant ccr orthologs. multiple alignment with other ...	2014	24723816
re-evaluation of the inheritance for root-knot nematode resistance in the upland cotton germplasm line m-120 rnr revealed two epistatic qtls conferring resistance.	we report a second major qtl for root-knot nematode resistance in the highly resistant upland cotton line m-120rnr and show epistasis between two resistant qtls with different mechanisms conferring resistance. in an earlier study, we identified a major qtl on chromosome 11 associated with resistance to root-knot nematode in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source. herein, we re-evaluated the genetics of the resistance to root-knot nematode in the m-1 ...	2014	24728014
within-field spatial distribution of stink bug (hemiptera: pentatomidae)-induced boll injury in commercial cotton fields of the southeastern united states.	spatial distribution of boll injury caused by stink bugs to developing cotton (gossypium hirsutum l.) bolls was studied in five commercial fields (≍22 ha each) in 2011 and 2012 to understand variability in boll injury dynamics within fields. cotton bolls and stink bugs were sampled weekly from a georeferenced grid of sampling points (one sample per 0.40 ha) in each field, but no samples were taken within 30 m of field edges. the inverse distance weighted interpolation, variogram analysis, and mo ...	2014	24735500
ghmpk17, a cotton mitogen-activated protein kinase, is involved in plant response to high salinity and osmotic stresses and aba signaling.	mitogen-activated protein kinase (mapk) cascades play pivotal roles in mediating biotic and abiotic stress responses. cotton (gossypium hirsutum) is the most important textile crop in the world, and often encounters abiotic stress during its growth seasons. in this study, a gene encoding a mitogen-activated protein kinase (mapk) was isolated from cotton, and designated as ghmpk17. the open reading frame (orf) of ghmpk17 gene is 1494 bp in length and encodes a protein with 497 amino acids. quanti ...	2014	24743296
ghwrky40, a multiple stress-responsive cotton wrky gene, plays an important role in the wounding response and enhances susceptibility to ralstonia solanacearum infection in transgenic nicotiana benthamiana.	wrky transcription factors form one of the largest transcription factor families and function as important components in the complex signaling processes that occur during plant stress responses. however, relative to the research progress in model plants, far less information is available on the function of wrky proteins in cotton. in the present study, we identified the ghwrky40 gene in cotton (gossypium hirsutum) and determined that the ghwrky40 protein is targeted to the nucleus and is a stres ...	2014	24747610
comparative transcriptome analysis of short fiber mutants ligon-lintless 1 and 2 reveals common mechanisms pertinent to fiber elongation in cotton (gossypium hirsutum l.).	understanding the molecular processes affecting cotton (gossypium hirsutum) fiber development is important for developing tools aimed at improving fiber quality. short fiber cotton mutants ligon-lintless 1 (li1) and ligon-lintless 2 (li2) are naturally occurring, monogenic mutations residing on different chromosomes. both mutations cause early cessation in fiber elongation. these two mutants serve as excellent model systems to elucidate molecular mechanisms relevant to fiber length development. ...	2014	24748059
waterlogging during flowering and boll forming stages affects sucrose metabolism in the leaves subtending the cotton boll and its relationship with boll weight.	the work explored sucrose metabolism in the leaves subtending the cotton boll (sbl) and its role in boll weight after waterlogging in cotton. results showed that net photosynthesis rate (pn), relative water content, contents of chlorophyll a and chlorophyll b, initial ribulose-1,5-bisphosphate carboxylase-oxygenase (rubisco) activity and cytosolic fructose-1, 6-bisphosphatase (cy-fbpase) activity decreased with waterlogging in the sbl on fruiting branches 2-3 (fb2-3) and fb6-7. activities of suc ...	2014	24767118
independent replication of mitochondrial genes supports the transcriptional program in developing fiber cells of cotton (gossypium hirsutum l.).	the mitochondrial genomes of flowering plants exist both as a "master circle" chromosome and as numerous subgenomic sublimons that are generated by intramolecular recombination. differential stability or replication of these sublimons allows individual mitochondrial gene copy numbers to vary independently between different cell types and developmental stages. our objective was to determine the relationship between mitochondrial gene copy number and transcript abundance in the elongating fiber ce ...	2014	24768176
lack of adaptation to a new host in a generalist herbivore: implications for host plant resistance to twospotted spider mites in cotton.	the twospotted spider mite (tetranychus urticae koch) is an important pest of cotton. this pest has a broad host range, but when changing between hosts an initial decline in fitness often occurs. this is usually followed by an increase in fitness after rapid adaptation to the new host, usually within five generations.	2015	24777962
molecular mapping and validation of a major qtl conferring resistance to a defoliating isolate of verticillium wilt in cotton (gossypium hirsutum l.).	verticillium wilt (vw) caused by verticillium dahliae kleb is one of the most destructive diseases of cotton. development and use of a vw resistant variety is the most practical and effective way to manage this disease. identification of highly resistant genes/qtl and the underlining genetic architecture is a prerequisite for developing a vw resistant variety. a major qtl qvw-c6-1 conferring resistance to the defoliating isolate v991 was identified on chromosome 6 in lhb22×jm11 f2∶3 population i ...	2014	24781706
pag1, a cotton brassinosteroid catabolism gene, modulates fiber elongation.	cotton (gossypium hirsutum) is the major source of natural textile fibers. brassinosteroids (brs) play crucial roles in regulating fiber development. the molecular mechanisms of brs in regulating fiber elongation, however, are poorly understood. pagoda1 (pag1) was identified via an activation tagging genetic screen and characterized by genome walking and brassinolide (bl) supplementation. rna-seq analysis was employed to elucidate the mechanisms of pag1 in regulating fiber development. pag1 exhi ...	2014	24786710
functional characterization of ghakt1, a novel shaker-like k⁺ channel gene involved in k⁺ uptake from cotton (gossypium hirsutum).	shaker-like potassium (k(+)) channels in plants play an important role in k(+) absorption and transport. in this study, we characterized a shaker-like k(+) channel gene ghakt1 from the roots of gossypium hirsutum cv. liaomian17. phylogenetic analysis showed that the ghakt1 belongs to the akt1-subfamily in the shaker-like k(+) channel family. confocal imaging of a ghakt1-green fluorescent fusion protein (gfp) in transgenic arabidopsis plants indicated that ghakt1 is localized in the plasma membra ...	2014	24802116
association mapping for epistasis and environmental interaction of yield traits in 323 cotton cultivars under 9 different environments.	improving yield is a major objective for cotton breeding schemes, and lint yield and its three component traits (boll number, boll weight and lint percentage) are complex traits controlled by multiple genes and various environments. association mapping was performed to detect markers associated with these four traits using 651 simple sequence repeats (ssrs). a mixed linear model including epistasis and environmental interaction was used to screen the loci associated with these four yield traits ...	2014	24810754
cotton knl1, encoding a class ii knox transcription factor, is involved in regulation of fibre development.	in this study, the ghknl1 (knotted1-like) gene, encoding a classical class ii knox protein was identified in cotton (gossypium hirsutum). ghknl1 was preferentially expressed in developing fibres at the stage of secondary cell wall (scw) biosynthesis. ghknl1 was localized in the cell nucleus, and could interact with ghofp4, as well as atofp1, atofp4, and atmyb75. however, ghknl1 lacked transcriptional activation activity. dominant repression of ghknl1 affected fibre development of cotton. the exp ...	2014	24831118
radiation and chemical mutagen induced somaclonal variations through in vitro organogenesis of cotton (gossypium hirsutum l.).	the purpose of the investigation was to induce somaclonal variations by gamma rays (gr), ethylmethane sulphonate (ems) and sodium azide (sa) during in vitro organogenesis of cotton.	2014	24831496
ghagl15s, preferentially expressed during somatic embryogenesis, promote embryogenic callus formation in cotton (gossypium hirsutum l.).	somatic embryogenesis is a useful tool for gene transfer and propagation of plants. agamous-like15 (agl15) promotes somatic embryogenesis in many plant species. in this study, three homologous agl15 genes were isolated from gossypium hirsutum l., namely ghagl15-1, ghagl15-3, and ghagl15-4. their putative proteins contained a highly conserved mads-box dna-binding domain and a less conserved k domain. phylogenetic analysis suggested that the three ghagl15s clustered most closely with agl15 protein ...	2014	24833045
elucidation of nuclear and organellar genomes of gossypium hirsutum: furthering studies of species evolution and applications for crop improvement.	plant genomes are larger and more complex than other eukaryotic organisms, due to small and large duplication events, recombination and subsequent reorganization of the genetic material. commercially important cotton is the result of a polyploidization event between old and new world cottons that occurred over one million years ago. allotetraploid cotton has properties that are dramatically different from its progenitors-most notably, the presence of long, spinnable fibers. recently, the complet ...	2013	24833222
diversity of endophytic fungi from different verticillium-wilt-resistant gossypium hirsutum and evaluation of antifungal activity against verticillium dahliae in vitro.	cotton plants were sampled and ranked according to their resistance to verticillium wilt. in total, 642 endophytic fungi isolates representing 27 genera were recovered from gossypium hirsutum root, stem, and leaf tissues, but were not uniformly distributed. more endophytic fungi appeared in the leaf (391) compared with the root (140) and stem (111) sections. however, no significant difference in the abundance of isolated endophytes was found among resistant cotton varieties. alternaria exhibited ...	2014	24836187
microrna-target gene responses to lead-induced stress in cotton (gossypium hirsutum l.).	micrornas (mirnas) play key roles in plant responses to various metal stresses. to investigate the mirna-mediated plant response to heavy metals, cotton (gossypium hirsutum l.), the most important fiber crop in the world, was exposed to different concentrations (0, 25, 50, 100, and 200 µm) of lead (pb) and then the toxicological effects were investigated. the expression patterns of 16 stress-responsive mirnas and 10 target genes were monitored in cotton leaves and roots by quantitative real-time ...	2014	24879091
quantitative trait loci analysis of fiber quality traits using a random-mated recombinant inbred population in upland cotton (gossypium hirsutum l.).	upland cotton (gossypium hirsutum l.) accounts for about 95% of world cotton production. improving upland cotton cultivars has been the focus of world-wide cotton breeding programs. negative correlation between yield and fiber quality is an obstacle for cotton improvement. random-mating provides a potential methodology to break this correlation. the suite of fiber quality traits that affect the yarn quality includes the length, strength, maturity, fineness, elongation, uniformity and color. iden ...	2014	24886099
protein expression changes during cotton fiber elongation in response to drought stress and recovery.	an investigation to better understand the molecular mechanism of cotton (gossypium hirsutum l.) fiber elongation in response to drought stress and recovery was conducted using a comparative proteomics analysis. cotton plants (cv. nucotn 33b) were subjected to water deprivation for 10 days followed by a recovery period (with watering) of 5 days. the temporal changes in total proteins in cotton fibers were examined using 2de. the results revealed that 163 proteins are significantly drought respons ...	2014	24889071
novel method to establish molecular identity using inter-simple sequence repeat markers in cotton (gossypium hirsutum) cultivars.	plant molecular identity (id) is used to describe molecular characteristics of plants, which should contain all of the necessary information. using inter-simple sequence repeat (issr) primers, molecular id can be described in a way that reflects the polymerase chain reaction (pcr) conditions, annealing temperature, and the bands obtained in pcr amplification. a new complete molecular id system is described in this study, which can be easily used and expanded to include more information. using th ...	2014	24938605
genome-wide analysis of the wrky gene family in cotton.	wrky proteins are major transcription factors involved in regulating plant growth and development. although many studies have focused on the functional identification of wrky genes, our knowledge concerning many areas of wrky gene biology is limited. for example, in cotton, the phylogenetic characteristics, global expression patterns, molecular mechanisms regulating expression, and target genes/pathways of wrky genes are poorly characterized. therefore, in this study, we present a genome-wide an ...	2014	24942461
in silico prediction of cotton (gossypium hirsutum) encoded micrornas targets in the genome of cotton leaf curl allahabad virus.	cotton leaf curl allahabad virus (clcuav) belongs to genus begomovirus, family geminiviridae. it has single stranded monopartite dna genome transmitted by whitefly (bemisia tabaci). micrornas (mirnas) belong to class of endogeneous small rnas which suppress expression of genes following cleavage or translational inhibition of target messenger rnas. they are demonstrated to be involved in a number of plant processes such as, development, biotic and abiotic stresses. employing in silico approach, ...	2014	24966530
carcass merit and meat quality in suffolk lambs, katahdin lambs, and meat-goat kids finished on a grass-legume pasture with and without supplementation.	the experiment evaluated traditional u.s. sheep (suffolk), hair sheep (katahdin), and meat goat (boer crossbred; goat) carcass and meat quality parameters when finished on pasture with and without supplemental whole cottonseed (gossypium hirsutum l.). supplemented animals had greater ribeye area (p<0.01), body wall thickness (p<0.001), and lean quality score (p<0.05) than unsupplemented animals. whole cottonseed increased fatty acids (fa) 18:1 trans-10, 18:1 trans-12, 18:2, and omega6:omega3 rat ...	2014	24971809
effect of simultaneous water deficit stress and meloidogyne incognita infection on cotton yield and fiber quality.	both water deficit stress and meloidogyne incognita infection can reduce cotton growth and yield, and drought can affect fiber quality, but the effect of nematodes on fiber quality is not well documented. to determine whether nematode parasitism affects fiber quality and whether the combined effects of nematode and drought stress on yield and quality are additive (independent effects), synergistic, or antagonistic, we conducted a study for 7 yr in a field infested with m. incognita. a split-plot ...	2014	24987162
is nectar reabsorption restricted by the stalk cells of floral and extrafloral nectary trichomes?	reabsorption is a phase of nectar dynamics that occurs concurrently with secretion; it has been described in floral nectaries that exude nectar through stomata or unicellular trichomes, but has not yet been recorded in extrafloral glands. apparently, nectar reabsorption does not occur in multicellular secretory trichomes (mst) due to the presence of lipophilic impregnations - which resemble casparian strips - in the anticlinal walls of the stalk cells. it has been assumed that these impregnation ...	2015	24987788
ghh2a12, a replication-dependent histone h2a gene from gossypium hirsutum, is negatively involved in the development of cotton fiber cells.	ghh2a12 was preferentially expressed at the initiation and early elongation stage of cotton fiber development, and overexpression of ghh2a12 caused retardation of fiber initiation and produced shorter fibers. histone h2a is a component of eukaryotic chromatin whose function has not been studied in cotton. we have isolated an h2a gene encoding 156 amino acids, named ghh2a12. like other plant histone h2as, ghh2a12 contains a typical spkk motif in the carboxy-terminal and a plant-unique peptide-bin ...	2014	25001001
predicting the effects of environment and management on cotton fibre growth and quality: a functional-structural plant modelling approach.	in general, the quality of fruits depends on local conditions experienced by the fruit during its development. in cotton, fruit quality, and more specifically the quality of the fibre in the fruit, depends on interactions between fruit position in the plant architecture, temperature and agronomic practices, such as sowing time, mulching with plastic film and topping of the plant's main stem and branches. to quantify this response of cotton fibre quality to environment and management, we develope ...	2014	25011385
in vitro cadmium-induced alterations in growth and oxidative metabolism of upland cotton (gossypium hirsutum l.).	cadmium (cd) is a toxic pollutant, which cause both dose- and time-dependent physiological and biochemical alterations in plants. the present in vitro study was undertaken to explore cd-induced physiological and biochemical changes in cotton callus culture at 0, 550, 700, 850, and 1000 μm cd for four different stress periods (7, 14, 21, and 28 days). at 1000 μm cd, mean growth values were lower than their respective control. the cell protein contents decreased only after 7-day and 14-day stress ...	2014	25013851
the effect of mepiquat chloride on elongation of cotton (gossypium hirsutum l.) internode is associated with low concentration of gibberellic acid.	the growth regulator mepiquat chloride (mc) is globally used in cotton (gossypium hirsutum l.) canopy manipulation to avoid excess growth and yield loss. however, little information is available as to whether the modification of plant architecture by mc is related to alterations in gibberellic acid (ga) metabolism and signaling. here, the role of ga metabolism and signaling was investigated in cotton seedlings treated with mc. the mc significantly decreased endogenous ga3 and ga4 levels in the e ...	2014	25017155
molecular analysis of proanthocyanidins related to pigmentation in brown cotton fibre (gossypium hirsutum l.).	the structural characteristics and component differences of proanthocyanidins in brown and white cotton fibres were identified by nuclear magnetic resonance (nmr) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (maldi-tof ms) analyses. proanthocyanidins in brown and white cotton fibres were found to contain mainly procyanidin (pc) and prodelphidin (pd) units with 2, 3-cis form (epigallocatechin and epicatechin). however, part of the proanthocyanidins in the white ...	2014	25086591
the entomopathogenic fungal endophytes purpureocillium lilacinum (formerly paecilomyces lilacinus) and beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions.	the effects of two entomopathogenic fungal endophytes, beauveria bassiana and purpureocillium lilacinum (formerly paecilomyces lilacinus), were assessed on the reproduction of cotton aphid, aphis gossypii glover (homoptera:aphididae), through in planta feeding trials. in replicate greenhouse and field trials, cotton plants (gossypium hirsutum) were inoculated as seed treatments with two concentrations of b. bassiana or p. lilacinum conidia. positive colonization of cotton by the endophytes was c ...	2014	25093505
transcriptome analysis of extant cotton progenitors revealed tetraploidization and identified genome-specific single nucleotide polymorphism in diploid and allotetraploid cotton.	the most widely cultivated cotton (gossypium hirsutum l., ad-genome) is derived from tetraploidization between a- and d-genome species. g. arboreum l. (a-genome) and g. raimondii ulbr. (d-genome) are two of closely-related extant progenitors. gene expression studies in allotetraploid cotton are complicated by the homoeologous loci of a- and d-genome origins. to develop genomic resources for gene expression and cotton breeding, we sequenced and assembled expressed sequence tags (ests) derived fro ...	2014	25099166
transcriptome and complexity-reduced, dna-based identification of intraspecies single-nucleotide polymorphisms in the polyploid gossypium hirsutum l.	varietal single nucleotide polymorphisms (snps) are the differences within one of the two subgenomes between different tetraploid cotton varieties and have not been practically used in cotton genetics and breeding because they are difficult to identify due to low genetic diversity and very high sequence identity between homeologous genes in cotton. we have used transcriptome and restriction site-associated dna sequencing to identify varietal snps among 18 g. hirsutum varieties based on the ratio ...	2014	25106949
next generation genetic mapping of the ligon-lintless-2 (li₂) locus in upland cotton (gossypium hirsutum l.).	mapping-by-sequencing and novel subgenome-specific snp markers were used to fine map the ligon-lintless 2 ( li 2 ) short-fiber gene in tetraploid cotton. these methodologies will accelerate gene identification in polyploid species. next generation sequencing offers new ways to identify the genetic mechanisms that underlie mutant phenotypes. the release of a reference diploid gossypium raimondii (d5) genome and bioinformatics tools to sort tetraploid reads into subgenomes has brought cotton genet ...	2014	25119870
effect of late planting and shading on cellulose synthesis during cotton fiber secondary wall development.	cotton-rapeseed or cotton-wheat double cropping systems are popular in the yangtze river valley and yellow river valley of china. due to the competition of temperature and light resources during the growing season of double cropping system, cotton is generally late-germinating and late-maturing and has to suffer from the coupling of declining temperature and low light especially in the late growth stage. in this study, late planting (lp) and shading were used to fit the coupling stress, and the ...	2014	25133819
a cotton (gossypium hirsutum) gene encoding a nac transcription factor is involved in negative regulation of plant xylem development.	nac proteins that compose of one large family of plant specific transcription factors (tf) play the important roles in many biological processes (such as morphogenesis, development, senescence and stress signal transduction). in this study, a gene (designated as ghxnd1) encoding a nac transcription factor was identified in cotton. sequence analysis indicated that ghxnd1 gene contains two introns inserted in its open reading frame (orf). ghxnd1 protein is localized in the cell nucleus, and displa ...	2014	25137291
physiological performance and differential expression profiling of genes associated with drought tolerance in contrasting varieties of two gossypium species.	cotton is mostly cultivated under rain-fed conditions in india, thus faces frequent drought conditions during its life cycle. drought being a major stress factor responsible for yield penalty, there has always been a high priority to generate knowledge on adaptation and tolerance of cotton. in the present study, four cotton varieties, jkc-770 and kc-2 (gossypium hirsutum), and jkc-717 and rahs-187(gossypium herbaceum), were imposed to drought. under drought condition, differential changes in phy ...	2015	25149149
water deficit in field-grown gossypium hirsutum primarily limits net photosynthesis by decreasing stomatal conductance, increasing photorespiration, and increasing the ratio of dark respiration to gross photosynthesis.	much effort has been expended to improve irrigation efficiency and drought tolerance of agronomic crops; however, a clear understanding of the physiological mechanisms that interact to decrease source strength and drive yield loss has not been attained. to elucidate the underlying mechanisms contributing to inhibition of net carbon assimilation under drought stress, three cultivars of gossypium hirsutum were grown in the field under contrasting irrigation regimes during the 2012 and 2013 growing ...	2014	25151126
proteomics profiling of fiber development and domestication in upland cotton (gossypium hirsutum l.).	comparative proteomic analyses were performed to detail the evolutionary consequences of strong directional selection for enhanced fiber traits in modern upland cotton (gossypium hirsutum l.). using two complementary proteomic approaches, 2-de and itraq lc-ms/ms, fiber proteomes were examined for four representative stages of fiber development. approximately 1,000 protein features were characterized using each strategy, collectively resulting in the identification and functional categorization o ...	2014	25156487
genomic organization, differential expression, and functional analysis of the spl gene family in gossypium hirsutum.	squamosa promoter binding protein-like (spl) genes encode plant-specific transcription factors that are involved in many fundamental developmental processes. certain spl genes contain sequences complementary to mir156, a microrna (mirna) that plays a role in modulating plant gene expression. in this study, 30 spl genes were identified in the reference genome of gossypium raimondii and 24 ghspls were cloned from gossypium hirsutum. g. raimondii is regarded as the putative contributor of the d-sub ...	2015	25159110
direct visualization of horizontal gene transfer in cotton plants.	plant mitochondrial and chloroplast genes that underwent horizontal transfer have been identified by parasite and grafting systems, respectively. here, we directly observed 3 horizontal gene transfer (hgt) events in the 45 second axillary shoots of grafted cotton plants (gossypium barbadense and gossypium hirsutum) after extirpating the first axillary bud. the second axillary shoots showed phenotypic variations in cotton flowers and seeds that were evidence of spontaneous development from cells ...	2014	25160847
ultrastructural, metabolic and proteomic changes in leaves of upland cotton in response to cadmium stress.	present study explores physiological, biochemical and proteomic changes in leaves of upland cotton (zms-49) using 500 μm cadmium (cd) along with control. leaves' biomass and chlorophyll pigments decreased at 500 μm cd. cd contents in roots were higher than leaves. levels of ros ( [formula: see text] and h2o2) both in vivo and in vitro and mda contents were significantly increased. chlorophyll parameters (f0, fm, fm(') and fv/fm), total soluble protein contents and apx showed a decline at 500 μm ...	2015	25169734
characterization of the global transcriptome for cotton (gossypium hirsutum l.) anther and development of ssr marker.	cotton is an important fiber plant, and it's attractive to elucidate the molecular mechanism of anther development due to the close relationship between the anther fertility and boll-setting, and also fiber yield. in the present paper, 47.2 million paired-end reads with average length of 82.87 bp from the anthers of tm-1 (gossypium hirsutum l.), a genetic standard line, were generated through transcriptome sequencing, and 210,965 unigenes of more than 100 bp were obtained. blast, kegg, cog, and ...	2014	25178523
quantitative trait loci mapping and genetic dissection for lint percentage in upland cotton (gossypium hirsutum).	lint percentage is an important character of cotton yield components and it is also correlated with cotton fibre development. in this study, we used a high lint percentage variety, baimian1, and a low lint percentage, tm-1 genetic standard for gossypium hirsutum, as parents to construct a mapping populations in upland cotton (g. hirsutum). a quantitative trait locus/loci (qtl) analysis of lint percentage was performed by using two mapping procedures; composite interval mapping (cim), inclusive c ...	2014	25189232
comprehensive analysis of differentially expressed genes and transcriptional regulation induced by salt stress in two contrasting cotton genotypes.	cotton (gossypium spp.) is one of the major fibre crops of the world. although it is classified as salt tolerant crop, cotton growth and productivity are adversely affected by high salinity, especially at germination and seedling stages. identification of genes and mirnas responsible for salt tolerance in upland cotton (gossypium hirsutum l.) would help reveal the molecular mechanisms of salt tolerance. we performed physiological experiments and transcriptome sequencing (mrna-seq and small rna-s ...	2014	25189468
determining the major bt refuge crops for cotton bollworm in north china.	evaluation of the effectiveness of refuge strategies involved in cotton bollworm bt resistance management would be aided by technologies that allow monitoring and quantification of key factors that affect the process under field conditions. we hypothesized that characterization of stable carbon and nitrogen isotopes in adult bollworm, helicoverpa armigera (hübner) (lepidoptera: noctuidae) moths may aid in determining the larval host that they developed upon. we found moths reared from larvae fed ...	2015	25196072
distribution and differentiation of wild, feral, and cultivated populations of perennial upland cotton (gossypium hirsutum l.) in mesoamerica and the caribbean.	perennial forms of gossypium hirsutum are classified under seven races. five mesoamerican races would have been derived from the wild race 'yucatanense' from northern yucatán. 'marie-galante', the main race in the caribbean, would have developed from introgression with g. barbadense. the racial status of coastal populations from the caribbean has not been clearly defined. we combined ecological niche modeling with an analysis of ssr marker diversity, to elucidate the relationships among cultivat ...	2014	25198534
genetic mapping of fiber color genes on two brown cotton cultivars in xinjiang.	in the present study, genetic linkage analysis was carried out to map the fiber color loci lc 1 and lc 2 on two brown cotton cultivars with ssr and est-ssr markers in the reference map by f2 segregation populations. the lc 1 locus carried by xincaimian6 (gossypium hirsutum l.) was flanked by the marker nau2862 and nau1043 on the long arm of chromosome 07, with genetic distance 7.8 cm and 3.8 cm, respectively. the lc 2 carried by xincaimian 5 (gossypium hirsutum l.) was flanked by the marker nau5 ...	2014	25207203
temperature responses of mesophyll conductance differ greatly between species.	the temperature responses of mesophyll conductance (gm ) were investigated for nine species using carbon isotope techniques combining tunable diode laser spectroscopy and gas exchange measurements. species included the evergreen trees eucalyptus pauciflora and quercus engelmannii; the tropical evergreen tree lophostemon confertus; as well as the herbaceous species nicotiana tabacum, oryza sativa, triticum aestivum, gossypium hirsutum, glycine max and arabidopsis thaliana. responses varied from a ...	2015	25224884
identification of centromeric regions on the linkage map of cotton using centromere-related repeats.	centromere usually contains high-copy-number retrotransposons and satellite repeats, which are difficult to map, clone and sequence. currently, very little is known about the centromere in cotton. here, we sequenced a bacterial artificial chromosome (bac) mapping to the centromeric region and predicted four long-terminal-repeat (ltr) retrotransposons. they were located in the heterochromatic centromeric regions of all 52 pachytene chromosomes in gossypium hirsutum. fiber-fish mapping revealed th ...	2014	25238895
effects of climate change on phenological trends and seed cotton yields in oasis of arid regions.	understanding the effects of climatic change on phenological phases of cotton (gossypium hirsutum l.) in oasis of arid regions may help optimize management schemes to increase productivity. this study assessed the impacts of climatic changes on the phenological phases and productivity of spring cotton. the results showed that climatic warming led the dates of sowing seed, seeding emergence, three-leaf, five-leaf, budding, anthesis, full bloom, cleft boll, boll-opening, boll-opening filling, and ...	2015	25240389
[genome-wide analysis of hsp70 superfamily in gossypium raimondii and the expression of orthologs in gossypium hirsutum].	heat shock 70 (hsp70) proteins are highly conserved molecular chaperones widely existed in the plant kingdom which are involved in cellular protein folding process. in this study, comprehensive evolutionary analyses of the gossypium raimondii hsp70 gene family members are conducted and 30 hsp70 genes are identified. the gene structure, chromosome distribution, gene duplication and phylogenic evolution of this family are further analyzed. the results reveal that hsp70 family genes can be clustere ...	2014	25252310
the cotton wrky transcription factor ghwrky17 functions in drought and salt stress in transgenic nicotiana benthamiana through aba signaling and the modulation of reactive oxygen species production.	drought and high salinity are two major environmental factors that significantly limit the productivity of agricultural crops worldwide. wrky transcription factors play essential roles in the adaptation of plants to abiotic stresses. however, wrky genes involved in drought and salt tolerance in cotton (gossypium hirsutum) are largely unknown. here, a group iid wrky gene, ghwrky17, was isolated and characterized. ghwrky17 was found to be induced after exposure to drought, salt, h2o2 and aba. the ...	2014	25261532
constructing a high-density linkage map for gossypium hirsutum × gossypium barbadense and identifying qtls for lint percentage.	to introgress the good fiber quality and yield from gossypium barbadense into a commercial upland cotton variety, a high-density simple sequence repeat (ssr) genetic linkage map was developed from a bc1 f1 population of gossypium hirsutum × gossypium barbadense. the map comprised 2,292 loci and covered 5115.16 centimorgan (cm) of the cotton ad genome, with an average marker interval of 2.23 cm. of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on ...	2015	25263268
transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae.	cotton is a major fibre crop grown worldwide that suffers extensive damage from chewing insects, including the cotton boll weevil larvae (anthonomus grandis). transcriptome analysis was performed to understand the molecular interactions between gossypium hirsutum l. and cotton boll weevil larvae. the illumina hiseq 2000 platform was used to sequence the transcriptome of cotton flower buds infested with boll weevil larvae.	2014	25280771
cotton lim domain-containing protein ghplim1 is specifically expressed in anthers and participates in modulating f-actin.	as one form of actin binding protein (abp), lim domain protein can trigger the formation of actin bundles during plant growth and development. in this study, a cdna (designated ghplim1) encoding a lim domain protein with 216 amino acid residues was identified from a cotton flower cdna library. quantitative rt-pcr indicated that ghplim1 is specifically expressed in cotton anthers, and its expression levels are regulated during anther development of cotton. ghplim1:egfp transformed cotton cells di ...	2015	25294521
cotton wrky1 mediates the plant defense-to-development transition during infection of cotton by verticillium dahliae by activating jasmonate zim-domain1 expression.	plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. previous research has demonstrated that della proteins physically interact with jasmonate zim-domain1 (jaz1) and dynamically regulate the interaction of the gibberellin (ga) and jasmonate (ja) signaling pathways. however, whether and how the jaz1-della regulatory node is regulated at the transcriptional level in plants under normal growth ...	2014	25301887
myb80 homologues in arabidopsis, cotton and brassica: regulation and functional conservation in tapetal and pollen development.	the arabidopsis atmyb80 transcription factor regulates genes involved in pollen development and controls the timing of tapetal programmed cell death (pcd). downregulation of atmyb80 expression precedes tapetal degradation. inhibition of atmyb80 expression results in complete male sterility. full-length atmyb80 homologs have been isolated in wheat, rice, barley and canola (c genome).	2014	25311582
mapping genomic loci for cotton plant architecture, yield components, and fiber properties in an interspecific (gossypium hirsutum l. × g. barbadense l.) ril population.	a quantitative trait locus (qtl) mapping was conducted to better understand the genetic control of plant architecture (pa), yield components (yc), and fiber properties (fp) in the two cultivated tetraploid species of cotton (gossypium hirsutum l. and g. barbadense l.). one hundred and fifty-nine genomic regions were identified on a saturated genetic map of more than 2,500 ssr and snp markers, constructed with an interspecific recombinant inbred line (ril) population derived from the genetic stan ...	2014	25314923
functional characterization of cotton genes responsive to verticillium dahliae through bioinformatics and reverse genetics strategies.	verticillium wilt causes dramatic cotton yield loss in china. although some genes or biological processes involved in the interaction between cotton and verticillium dahliae have been identified, the molecular mechanism of cotton resistance to this disease is still poorly understood. the basic innate immune response for defence is somewhat conserved among plant species to defend themselves in complex environments, which makes it possible to characterize genes involved in cotton immunity based on ...	2014	25326626
overexpression of a cotton annexin gene, ghann1, enhances drought and salt stress tolerance in transgenic cotton.	plant annexins are members of a diverse, multigene protein family that has been associated with a variety of cellular processes and responses to abiotic stresses. ghann1, which encodes a putative annexin protein, was isolated from a cotton (gossypium hirsutum l. acc 7235) cdna library. tissue-specific expression showed that ghann1 is expressed at differential levels in all tissues examined and strongly induced by various phytohormones and abiotic stress. in vivo and in vitro subcellular localiza ...	2015	25330941
identification of novel micrornas in the verticillium wilt-resistant upland cotton variety kv-1 by high-throughput sequencing.	plant micrornas (mirnas) play essential roles in the post-transcriptional regulation of gene expression during development, flowering, plant growth, metabolism, and stress responses. verticillium wilt is one of the vascular disease in plants, which is caused by the verticillium dahlia and leads to yellowing, wilting, lodging, damage to the vascular tissue, and death in cotton plants. upland cotton varieties kv-1 have shown resistance to verticillium wilt in multiple levels. however, the knowledg ...	2014	25332864
development and bin mapping of gene-associated interspecific snps for cotton (gossypium hirsutum l.) introgression breeding efforts.	cotton (gossypium spp.) is the largest producer of natural fibers for textile and is an important crop worldwide. crop production is comprised primarily of g. hirsutum l., an allotetraploid. however, elite cultivars express very small amounts of variation due to the species monophyletic origin, domestication and further bottlenecks due to selection. conversely, wild cotton species harbor extensive genetic diversity of prospective utility to improve many beneficial agronomic traits, fiber charact ...	2014	25359292
itraq protein profile differential analysis between somatic globular and cotyledonary embryos reveals stress, hormone, and respiration involved in increasing plantlet regeneration of gossypium hirsutum l.	somatic embryo development (sed) in upland cotton shows low frequencies of embryo maturation and plantlet regeneration. progress in increasing the regeneration rate has been limited. here a global analysis of proteome dynamics between globular and cotyledonary embryos was performed using isobaric tags for relative and absolute quantitation to explore mechanisms underlying sed. of 6318 proteins identified by a mass spectrometric analysis, 102 proteins were significantly up-regulated and 107 were ...	2015	25367710
cotton (gossypium hirsutum) 14-3-3 proteins participate in regulation of fibre initiation and elongation by modulating brassinosteroid signalling.	cotton (gossypium hirsutum) fibre is an important natural raw material for textile industry in the world. understanding the molecular mechanism of fibre development is important for the development of future cotton varieties with superior fibre quality. in this study, overexpression of gh14-3-3l in cotton promoted fibre elongation, leading to an increase in mature fibre length. in contrast, suppression of expression of gh14-3-3l, gh14-3-3e and gh14-3-3h in cotton slowed down fibre initiation and ...	2015	25370928
evaluation of cotton leaf curl virus resistance in bc1, bc2, and bc3 progenies from an interspecific cross between gossypium arboreum and gossypium hirsutum.	cotton leaf curl virus disease (clcud) is an important constraint to cotton production. the resistance of g. arboreum to this devastating disease is well documented. in the present investigation, we explored the possibility of transferring genes for resistance to clcud from g. arboreum (2n = 26) cv 15-mollisoni into g. hirsutum (2n = 52) cv crsm-38 through conventional breeding. we investigated the cytology of the bc1 to bc3 progenies of direct and reciprocal crosses of g. arboreum and g. hirsut ...	2014	25372141
overexpression of mic-3 indicates a direct role for the mic gene family in mediating upland cotton (gossypium hirsutum) resistance to root-knot nematode (meloidogyne incognita).	transgene-based analysis of the mic-3 gene provides the first report of a cotton gene having a direct role in mediating cotton resistance to root-knot nematode. major quantitative trait loci have been mapped to upland cotton (gossypium hirsutum l.) chromosomes 11 and 14 that govern the highly resistant phenotype in response to infection by root-knot nematode (rkn; meloidogyne incognita); however, nearly nothing is known regarding the underlying molecular determinants of this rkn-resistant phenot ...	2015	25376794
genome-wide cloning, identification, classification and functional analysis of cotton heat shock transcription factors in cotton (gossypium hirsutum).	heat shock transcriptional factors (hsfs) play important roles in the processes of biotic and abiotic stresses as well as in plant development. cotton (gossypium hirsutum, 2n=4x=(ad)2=52) is an important crop for natural fiber production. due to continuous high temperature and intermittent drought, heat stress is becoming a handicap to improve cotton yield and lint quality. recently, the related wild diploid species gossypium raimondii genome (2n=2x=(d5)2=26) has been fully sequenced. in order t ...	2014	25378022
morphological and physiological responses of cotton (gossypium hirsutum l.) plants to salinity.	salinization usually plays a primary role in soil degradation, which consequently reduces agricultural productivity. in this study, the effects of salinity on growth parameters, ion, chlorophyll, and proline content, photosynthesis, antioxidant enzyme activities, and lipid peroxidation of two cotton cultivars, [ccri-79 (salt tolerant) and simian 3 (salt sensitive)], were evaluated. salinity was investigated at 0 mm, 80 mm, 160 mm, and 240 mm nacl for 7 days. salinity induced morphological and ph ...	2014	25391141
global microrna modification in cotton (gossypium hirsutum l.).	micrornas (mirnas) are small noncoding rnas participating in versatile biological processes via post-transcriptionally gene regulation. however, how mirnas are modified or degraded remains unknown, despite years of studies have unravelled much details of mirna biogenesis and function. here, we systematically investigated mirna modification using six small rna sequencing libraries generated from cotton seedling as well as cotton fibre at five developmental stages. our results show that 1-2-nt tru ...	2015	25393285
cloning of gossypium hirsutum sucrose non-fermenting 1-related protein kinase 2 gene (ghsnrk2) and its overexpression in transgenic arabidopsis escalates drought and low temperature tolerance.	the molecular mechanisms of stress tolerance and the use of modern genetics approaches for the improvement of drought stress tolerance have been major focuses of plant molecular biologists. in the present study, we cloned the gossypium hirsutum sucrose non-fermenting 1-related protein kinase 2 (ghsnrk2) gene and investigated its functions in transgenic arabidopsis. we further elucidated the function of this gene in transgenic cotton using virus-induced gene silencing (vigs) techniques. we hypoth ...	2014	25393623
molecular markers and cotton genetic improvement: current status and future prospects.	narrow genetic base and complex allotetraploid genome of cotton (gossypium hirsutum l.) is stimulating efforts to avail required polymorphism for marker based breeding. the availability of draft genome sequence of g. raimondii and g. arboreum and next generation sequencing (ngs) technologies facilitated the development of high-throughput marker technologies in cotton. the concepts of genetic diversity, qtl mapping, and marker assisted selection (mas) are evolving into more efficient concepts of ...	2014	25401149
genome-wide analysis of the omega-3 fatty acid desaturase gene family in gossypium.	the majority of commercial cotton varieties planted worldwide are derived from gossypium hirsutum, which is a naturally occurring allotetraploid produced by interspecific hybridization of a- and d-genome diploid progenitor species. while most cotton species are adapted to warm, semi-arid tropical and subtropical regions, and thus perform well in these geographical areas, cotton seedlings are sensitive to cold temperature, which can significantly reduce crop yields. one of the common biochemical ...	2014	25403726
ghdrin1, a novel drought-induced gene of upland cotton (gossypium hirsutum l.) confers abiotic and biotic stress tolerance in transgenic tobacco.	a novel stress tolerance cdna fragment encoding ghdrin1 protein was identified and its regulation was studied in cotton boll tissues and seedlings subjected to various biotic and abiotic stresses. phylogenetic and conserved domain prediction indicated that ghdrin1 was annotated with a hypothetical protein of unknown function. subcellular localization showed that ghdrin1 is localized in the chloroplasts. the promoter sequence was isolated and subjected to in silico study. various cis-acting eleme ...	2015	25413882
cotton (gossypium hirsutum l.).	cotton continues to be a crop of great economic importance in many developing and some developed countries. cotton plants expressing the bt gene to deter some of the major pests have been enthusiastically and widely accepted by the farmers in three of the major producing countries, i.e., china, india, and the usa. considering the constraints related to its production and the wide variety of products derived from the cotton plant, it offers several target traits that can be improved through genet ...	2015	25416245
molecular cloning and functional analysis of the flowering locus t (ft) homolog ghft1 from gossypium hirsutum.	flowering locus t (ft) encodes a member of the phosphatidylethanolamine-binding protein (pebp) family that functions as the mobile floral signal, playing an important role in regulating the floral transition in angiosperms. we isolated an ft-homolog (ghft1) from gossypium hirsutum l. cultivar, xinluzao 33 ghft1 was predominantly expressed in stamens and sepals, and had a relatively higher expression level during the initiation stage of fiber development. ghft1 mrna displayed diurnal oscillations ...	2015	25429737
identification and profiling of known and novel fiber micrornas during the secondary wall thickening stage in cotton (gossypium hirsutum) via high-throughput sequencing.		2014	25438700
development of est-based snp and indel markers and their utilization in tetraploid cotton genetic mapping.	availability of molecular markers has proven to be an efficient tool in facilitating progress in plant breeding, which is particularly important in the case of less researched crops such as cotton. considering the obvious advantages of single nucleotide polymorphisms (snps) and insertion-deletion polymorphisms (indels), expressed sequence tags (ests) were analyzed in silico to identify snps and indels in this study, aiming to develop more molecular markers in cotton.	2014	25442170
quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation.	an itraq-based proteomics of ovules from the upland cotton species gossypium hirsutum and its fuzzless-lintless mutant was performed, and finally 2729 proteins that preferentially accumulated at anthesis in wild-type ovules were identified. we confirmed that the gene expression levels of 2005 among these proteins also increased by performing an rna sequencing transcriptomics. expression of proteins involved in carboxylic acid metabolism, small-molecule metabolic processes, hormone regulation, an ...	2015	25449837
a comparative meta-analysis of qtl between intraspecific gossypium hirsutum and interspecific g. hirsutum × g. barbadense populations.	based on 1075 and 1059 qtl from intraspecific upland and interspecific upland × pima populations, respectively, the identification of qtl clusters and hotspots provides a useful resource for cotton breeding. mapping of quantitative trait loci (qtl) is a pre-requisite of marker-assisted selection for crop yield and quality. recent meta-analysis of qtl in tetraploid cotton (gossypium spp.) has identified regions of the genome with high concentrations of qtl for various traits called clusters and s ...	2015	25501533
silicon (si) alleviates cotton (gossypium hirsutum l.) from zinc (zn) toxicity stress by limiting zn uptake and oxidative damage.	silicon (si) is as an important fertilizer element, which has been found effective in enhancing plant tolerance to variety of biotic and a-biotic stresses. this study investigates the si potential to alleviate zinc (zn) toxicity stress in cotton (gossypium hirsutum l.). cotton plants were grown in hydroponics and exposed to different zn concentration, 0, 25, and 50 μm, alone and/or in combination with 1 mm si. incremental zn concentration in growth media instigated the cellular oxidative damage ...	2015	25516248
a r2r3-myb transcription factor that is specifically expressed in cotton (gossypium hirsutum) fibers affects secondary cell wall biosynthesis and deposition in transgenic arabidopsis.	secondary cell wall (scw) is an important industrial raw material for pulping, papermaking, construction, lumbering, textiles and potentially for biofuel production. the process of scw thickening of cotton fibers lays down the cellulose that will constitute the bulk (up to 96%) of the fiber at maturity. in this study, a gene encoding a myb-domain protein was identified in cotton (gossypium hirsutum) and designated as ghmybl1. quantitative real-time polymerase chain reaction (rt-pcr) analysis rev ...	2015	25534543