Publications

identification of two novel regulatory genes involved in pristinamycin biosynthesis and elucidation of the mechanism for atra-p-mediated regulation in streptomyces pristinaespiralis.	in this study, using a transposon-based strategy, two novel regulatory genes were identified as being involved in the biosynthesis of both pristinamycin i (pi) and ii (pii) in streptomyces pristinaespiralis, including a tetr-family regulatory gene atra-p (ssdg_00466) and an orphan histidine kinase gene ssdg_02492. the mechanism by which atra-p exerted a positive role in pristinamycin production was elucidated. we showed that deletion of atra-p resulted in a delayed production of both pi and pii ...	2015	25957493
estimation of intestinal protein digestibility of protein supplements for ruminants using a three-step enzymatic in vitro procedure.	this study included 33 samples with main focus on unprotected or rumen-protected rapeseed and soybean feedstuffs, which were analysed using an enzymatic in vitro procedure (eivp) in order to determine intestinal crude protein (cp) digestibility (ipd) of ruminally undegraded cp. the eivp involved the sequential digestion of samples with a protease from streptomyces griseus, pepsin-hcl and pancreatin. briefly, the eivp started with determination of true protein. feeds were incubated for 18 h in a ...	2015	26054973
a simple and efficient method of slow freezing for human embryonic stem cells and induced pluripotent stem cells.	protocols available for the cryopreservation of human embryonic stem (es) and induced pluripotent stem (ips) cells are very inefficient and laborious compared to those for the cryopreservation of murine es/ips cells or other general cell lines. while the vitrification method may be adequate when working with small numbers of human es/ips cells, it requires special skills and is unsuitable when working with large cell numbers. here, we describe a simple and efficient method for the cryopreservati ...	2016	26069023
enhanced detection of post-transcriptional modifications using a mass-exclusion list strategy for rna modification mapping by lc-ms/ms.	there has been a renewed appreciation for the dynamic nature of ribonucleic acid (rna) modifications and for the impact of modified rnas on organism health resulting in an increased emphasis on developing analytical methods capable of detecting modifications within specific rna sequence contexts. here we demonstrate that a dna-based exclusion list enhances data dependent liquid chromatography tandem mass spectrometry (lc-ms/ms) detection of post-transcriptionally modified nucleosides within spec ...	2015	26176336
l-asparaginase from streptomyces griseus niot-vkma29: optimization of process variables using factorial designs and molecular characterization of l-asparaginase gene.	marine actinobacteria are known to be a rich source for novel metabolites with diverse biological activities. in this study, a potential extracellular l-asparaginase was characterised from the streptomyces griseus niot-vkma29. box-behnken based optimization was used to determine the culture medium components to enhance the l-asparaginase production. ph, starch, yeast extract and l-asparagine has a direct correlation for enzyme production with a maximum yield of 56.78 iu ml(-1). a verification ex ...	2015	26206135
3-amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant corynebacterium glutamicum.	the production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-ahba) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. recombinant corynebacterium glutamicum strain kt01 expressing grih and grii derived from streptomyces griseus produced 3,4-ahba from the sweet sorghum juice of cultivar sil-05 at a final concentration (1.0 ...	2015	26409852
antitumor compounds from streptomyces sp. kml-2, isolated from khewra salt mines, pakistan.	actinomycetes are gram positive bacteria with high g + c content in their dna and are capable of producing variety of secondary metabolites. many of these metabolites possess different biological activities and have the potential to be developed as therapeutic agents. the aim of the present study was to screen actinomycetes inhabiting halophilic environment such as khewra salt mines present in pakistan for cytotoxic and antitumor compounds.	2015	26466995
biofiltration technology for the removal of toluene from polluted air using streptomyces griseus.	biofiltration technology has been recognized as a promising biotechnology for treating the volatile organic compounds (vocs) present in polluted air. this study aims to investigate the performance of a biofiltration system of streptomyces griseus sp. dsm-40759 immobilized on activated carbon (pica s23) towards the adsorption and degradation of toluene vapour as well as to regenerate the activated carbon in situ. the batch studies were performed using nutrient agar medium and basal salt medium (b ...	2016	26469210
a branch point of streptomyces sulfur amino acid metabolism controls the production of albomycin.	albomycin (abm), also known as grisein, is a sulfur-containing metabolite produced by streptomyces griseus atcc 700974. genes predicted to be involved in the biosynthesis of abm and abm-like molecules are found in the genomes of other actinomycetes. abm has potent antibacterial activity, and as a result, many attempts have been made to develop abm into a drug since the last century. although the productivity of s. griseus can be increased with random mutagenesis methods, understanding of strepto ...	2015	26519385
iminimycin a, the new iminium metabolite produced by streptomyces griseus os-3601.	a new natural product, designated iminimycin a, was isolated from the cultured broth of a streptomycin-producing microbial strain, streptomyces griseus os-3601, via a physicochemical screening method using hp-20, silica gel and ods column chromatographies and subsequent preparative hplc. iminimycin a is an indolizidine alkaloid, containing of an unusual iminium group and a cyclopropane ring with a triene side chain. the absolute configuration of iminimycin a was elucidated by nmr studies and ele ...	2016	26758492
a pyridoxal phosphate-dependent enzyme that oxidizes an unactivated carbon-carbon bond.	pyridoxal 5'-phosphate (plp)-dependent enzymes have wide catalytic versatility but are rarely known for their ability to react with oxygen to catalyze challenging reactions. here, using in vitro reconstitution and kinetic analysis, we report that the indolmycin biosynthetic enzyme ind4, from streptomyces griseus atcc 12648, is an unprecedented o2- and plp-dependent enzyme that carries out a four-electron oxidation of l-arginine, including oxidation of an unactivated carbon-carbon (c-c) bond. we ...	2016	26807714
treatment of clostridium difficile infection using sq641, a capuramycin analogue, increases post-treatment survival and improves clinical measures of disease in a murine model.	clostridium difficile infection (cdi) is a primary cause of antibiotic-associated diarrhoeal illness. current therapies are insufficient as relapse rates following antibiotic treatment range from 25% for initial treatment to 60% for treatment of recurrence. in this study, we looked at the efficacy of sq641 in a murine model of cdi. sq641 is an analogue of capuramycin, a naturally occurring nucleoside-based compound produced by streptomyces griseus.	2016	26832756
naturally occurring isoleucyl-trna synthetase without trna-dependent pre-transfer editing.	isoleucyl-trna synthetase (ilers) is unusual among aminoacyl-trna synthetases in having a trna-dependent pre-transfer editing activity. alongside the typical bacterial ilers (such as escherichia coli ilers), some bacteria also have the enzymes (eukaryote-like) that cluster with eukaryotic ilerss and exhibit low sensitivity to the antibiotic mupirocin. our phylogenetic analysis suggests that the iles1 and iles2 genes of contemporary bacteria are the descendants of genes that might have arisen by ...	2016	26921320
grisemycin, a bridged angucyclinone with a methylsulfinyl moiety from a marine-derived streptomyces sp.	grisemycin (1), the first sulfur angucyclinone with an unusual ether-bridged system, was isolated from a marine-derived streptomyces griseus strain m268. its novel, here cage-like, structure was determined by spectroscopic analysis and single-crystal x-ray diffraction. compound 1 exhibited modestly selective activity against the hl-60 cell line with an ic50 value of 31.54 μm. futhermore, the absolute stereochemistry of kiamycin (2), an 1,12-epoxybenz[a]anthracene, previously obtained from the sa ...	2016	26958869
improved production of active streptomyces griseus trypsin with a novel auto-catalyzed strategy.	n-terminal sequences play crucial roles in regulating expression, translation, activation and enzymatic properties of proteins. to reduce cell toxicity of intracellular trypsin and increase secretory expression, we developed a novel auto-catalyzed strategy to produce recombinant trypsin by engineering the n-terminus of mature streptomyces griseus trypsin (sgt). the engineered n-terminal peptide of sgt was composed of the thioredoxin, glycine-serine linker, his6-tag and the partial bovine trypsin ...	2016	26983398
marine-derived secondary metabolite, griseusrazin a, suppresses inflammation through heme oxygenase-1 induction in activated raw264.7 macrophages.	a new secondary metabolite, named griseusrazin a (1), was isolated from the marine-derived bacterium streptomyces griseus subsp. griseus. the structure of the compound was determined by analysis of spectroscopic data including ms, cosy, hsqc, hmbc, and (15)n-hmbc data. griseusrazin a (1) inhibited the production of inflammatory mediators, such as prostaglandin e2 and nitric oxide, which was mediated through the suppression of the expression of cyclooxygenase-2 (cox-2) and inducible nitric oxide ...	2016	27019105
evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants against sclerotium rolfsii in chickpea.	to evaluate the potential of chitinolytic endophytic actinomycetes isolated from medicinal plants in order to diminish the collar rot infestation induced by sclerotium rolfsii in chickpea.	2016	27170067
an abc transporter involved in the control of streptomycin production in streptomyces griseus.	we screened for a gene that inhibits streptomycin production in streptomyces griseus when it is introduced on a high-copy-number plasmid pij702, and obtained a plasmid pkm545. the introduction of pkm545 abolished streptomycin production on all media tested including ymp-sugar and nutrient broth. s1 protection analysis demonstrated that the introduction of this plasmid downregulated the transcriptional activity of the promoter preceding strr, the pathway-specific transcriptional regulator for str ...	2016	27268270
effects of organic solvents on streptomyces griseus protease i with transfer action.	the protease i from streptomyces griseus var. alcalophilus strongly catalyzed the transfer reaction forming hydroxamic acids of various amino acids, especially aromatic and hydrophobic amino acids. dimethyl sulfoxide (dmso), 1,4-butane diol, and glycerol protected this enzyme from heat denaturation. moreover, only dmso strongly activated the transfer action of protease i and the velocity of reaction was accelerated three times in the presence of 50% dmso. the yield of transfer product strongly d ...	1992	27280814
the production of flocculating substance(s) by streptomyces griseus.	streptomyces griseus produced a certain kind of flocculating substance(s). the flocculating substance(s) could aggregate suspended kaolin particles in solution to form small floes and enables it to sediment those particles. the production of flocculating substance(s) by s. griseus was not parallel to the cell growth and a large number of flocculating substance(s) was released into the culture broth in the death phase. the flocculating substance(s) produced by s. griseus acted under acidic condit ...	1996	27299553
efficient production of nonactin by streptomyces griseus subsp. griseus.	here we report the production of the cyclic macrotetrolide nonactin from the fermentation culture of streptomyces griseus subsp. griseus. nonactin is a member of a family of naturally occurring cyclic ionophores known as the macrotetrolide antibiotics. our fermentation procedure of streptomyces griseus was performed at 30 °c and 200 rev·min(-1) for 5 days on a rotary shaker. diaion hp-20 and amberlite xad-16 were added to the fermentation medium. isolated yield of nonactin was up to 80 mg·l(-1) ...	2016	27405846
isolation and evaluation of proteolytic actinomycete isolates as novel inducers of pearl millet downy mildew disease protection.	native endophytic actinomycetes isolated from pearl millet roots were examined for their efficacy to protect pearl millet against downy mildew. nineteen of 39 isolates were found to be proteolytic, of which 7 strains could directly suppress the sporangium formation of sclerospora graminicola, the pearl millet downy mildew pathogen. thus, mycelial suspensions containing either spores or cell-free extract of these 7 isolates were used for seed-coating and -soaking treatments to test for their indu ...	2016	27499196
production of n-acetyl-d-glucosamine from mycelial waste by a combination of bacterial chitinases and an insect n-acetyl-d-glucosaminidase.	n-acetyl-d-glucosamine (glcnac) has great potential to be used as a food additive and medicine. the enzymatic degradation of chitin-containing biomass for producing glcnac is an eco-friendly approach but suffers from a high cost. the economical efficiency can be improved by both optimizing the member and ratio of the chitinolytic enzymes and using new inexpensive substrates. to address this, a novel combination of bacterial and insect chitinolytic enzymes was developed in this study to efficient ...	2016	27546481
high production of a class iii lantipeptide amfs in streptomyces griseus.	amfs, a class iii lantipeptide serves as a morphogen in streptomyces griseus. here, we constructed a high production system of amfs in s. griseus. we isolated s. griseus grd1 strain defective in glucose repression of aerial mycelium formation and found it suitable for the overproduction of amfs. two expression vectors carrying the strong and constitutive erme2 promoter were constructed using a multicopy number plasmid, pij702. the use of the grd1 strain combined with the expression vectors enabl ...	2017	27691921
biosynthesis of optically pure chiral alcohols by a substrate coupled and biphasic system with a short-chain dehydrogenase from streptomyces griseus.	the increasing demand for biocatalysts in synthesizing enantiomerically pure chiral alcohols results from the outstanding characteristics of enzymes in reaction, economic, ecological issues. many carbonyl reductases for producing chiral alcohols have been reported but there is still a lack of good catalytic efficacies. herein, five carbonyl reductases from different streptomyces were discovered by the strategy of genome mining. these reductases were overexpressed, and we chose sgcr for further s ...	2016	27702481
complete genome sequence of the streptomyces phage nanodon.	streptomyces phage nanodon is a temperate double-stranded dna siphoviridae belonging to cluster bd1. it was isolated from soil collected in kilauea, hi, using streptomyces griseus subsp. griseus as a host.	2016	27795236
new and highly active microbial phosphotriesterase sources.	many toxic insecticides used worldwide as well as some chemical warfare agents are phosphotriester derivatives. therefore, detoxification of organophosphorus compounds has become the subject of many studies and in particular bioremediation, based on the phosphotriesterase catalysed hydrolysis of these compounds, has shown to be an effective and ecological methodology. in order to identify new bacterial phosphotriesterases, a simple and sensitive fluorimetric screening method on solid media was e ...	2016	27915249
protein acetylation involved in streptomycin biosynthesis in streptomyces griseus.	protein acetylation, the reversible addition of an acetyl group to lysine residues, is a protein post-translational modification ubiquitous in living cells. although the involvement of protein acetylation in the regulation of primary metabolism has been revealed, the function of protein acetylation is largely unknown in secondary metabolism. here, we characterized protein acetylation in streptomyces griseus, a streptomycin producer. protein acetylation was induced in the stationary and sporulati ...	2017	28034645
β-glucosidase from streptomyces griseus: nanoparticle immobilisation and application to alkyl glucoside synthesis.	a novel β-glucosidase from streptomyces griseus was cloned and overexpressed in e. coli. the purified β-glucosidase (44 kda) had a km of 8.6 ± 0.5 mm and a vmax of 217 ± 5.0 μmoles(-1)min(-1)mg at 37 °c, ph 7.2 with p-nitrophenyl-β-d glucopyranoside as substrate. the enzyme was characterised in terms of ph optimum (ph 6.9), temperature optimum (69 °c) and the influence of solvents and effectors. purified s. griseus β-glucosidase was successfully immobilised, by simple absorption, onto zinc oxide ...	2017	28161545
79 the cell-cycle related nuclear localization of platelet-activating factor in bovine and murine embryo development.	platelet-activating factor (paf) is a lipid mediator produced by a wide variety of cells and well known for its involvement in host defense and platelet aggregation. in embryo development, paf functions as an embryotropin and a marker of embryo quality and viability in human and mice. however, only limited information is available concerning its function during in vitro culture of bovine embryos. bovine and murine embryos were collected at different time points according to their development (4- ...	2016	28278876
characterization of bafilomycin biosynthesis in kitasatospora setae km-6054 and comparative analysis of gene clusters in actinomycetales microorganisms.	bafilomycins a1, c1 and b1 (setamycin) produced by kitasatospora setae km-6054 belong to the plecomacrolide family, which exhibit antibacterial, antifungal, antineoplastic and immunosuppressive activities. an analysis of gene clusters from k. setae km-6054 governing the biosynthesis of bafilomycins revealed that it contains five large open reading frames (orfs) encoding the multifunctional polypeptides of bafilomycin polyketide synthases (pkss). these clustered pks genes, which are responsible f ...	2017	28293034
bioresolution of racemic phenyl glycidyl ether by a putative recombinant epoxide hydrolase from streptomyces griseus nbrc 13350.	in order to produce enantiomerically pure epoxides for the synthesis of value-added chemicals, a novel putative epoxide hydrolase (eh) sgeh was cloned and overexpressed in pet28a/escherichia coli bl21(de3). the 1047 bp sgeh gene was mined from streptomyces griseus nbrc 13350 genome sequence. the recombinant hexahistidyl-tagged sgeh was purified (16.6-fold) by immobilized metal-affinity chromatography, with 90% yield as a homodimer of 100 kda. the recombinant e. coli whole cells overexpressing sg ...	2017	28378221