Publications

bmy 28100, a new oral cephalosporin.	bmy 28100, a new oral cephalosporin with a (z)-propenyl side chain at the 3 position and a p-hydroxyphenylglycyl substituent at the 7 position, was evaluated in comparison with cefaclor and cephalexin and, when appropriate, ampicillin and vancomycin. in vitro, bmy 28100 was more active than the reference cephalosporins against streptococci, staphylococcus aureus, staphylococcus epidermidis, listeria monocytogenes, haemophilus influenzae, propionibacterium acnes, clostridium perfringens, and clos ...	1987	3105449
frequency analysis of augmented ctl production associated with corynebacterium parvum-induced tumour regression.	a limiting-dilution frequency assay was employed to estimate the increased production of cytolytic t lymphocytes (ctl) associated with corynebacterium parvum-induced regression of the p815 mastocytoma growing subcutaneously in semisyngeneic mice. it was found that intratumour c. parvum functioned to augment greatly the underlying concomitant production of ctl that occurs normally in response to a progressively growing untreated immunogenic tumour. the lymph node draining a c. parvum-treated tumo ...	1987	3106195
propionibacteria isolates and asymptomatic infections of the peritoneal effluent in capd patients.	during the past two years our laboratory has isolated propionibacterium-an anaerobic gram-positive rod-on 40 occasions from the drainage fluid of 25 patients on capd and eight on ipd. most patients had no symptoms and the fluid was sent for culture after treatment of peritonitis, during training period, and occasionally because it was cloudy. no association was found with patients' age, length on dialysis, or previous peritonitis episodes. the only statistically significant association (p = 0.03 ...	1986	3110672
therapeutic effect of cell-wall skeleton of propionibacterium acnes in combination with a monoclonal antibody (c6-1.2) on the lung metastases of lewis lung carcinoma.	the purpose of this study was to determine whether the combination of c6-1.2 monoclonal antibody (moab) (established in our laboratory), which is specifically reactive with lewis lung carcinoma (3ll), and cell-wall skeleton of propionibacterium acnes c7 (p. acnes-cws) would significantly decrease established spontaneous metastases of 3ll. c6-1.2 moab combined with intratumoral (or intralesional) injection of p. acnes-cws, when administered at an early stage of the experiment, showed a significan ...	1987	3112064
the effect of carbon dioxide on the in vitro activity of erythromycin and ru-28965 against anaerobic bacteria.	the in vitro activity of erythromycin and ru-28965 (a novel macrolide antimicrobial with improved pharmacokinetics) was determined against a variety of anaerobic bacteria in anaerobic atmospheres with and without added carbon dioxide. minimum inhibitory concentrations (mic) were determined using an antimicrobial incorporation technique in wilkins-chalgren agar (oxoid, uk) containing saponinlysed horse blood to a final concentration of 10%. the inoculum used was approximately 10(4) colony forming ...	1987	3113907
plasma protein synthesis in experimental cancer compared to paraneoplastic conditions, including monokine administration.	during tumor growth, there are characteristic alterations in the concentration and synthesis of various plasma proteins. the purpose of this study was to evaluate whether these changes are unique to a tumor-bearing state, or rather, they represent a generalized response to a paraneoplastic state mediated by the release of monokines or protein-calorie malnutrition. plasma protein synthesis and concentrations in mice bearing a transplantable fibrosarcoma were compared to animals receiving either a ...	1987	3117356
incomplete surgery, local immunostimulation, and recurrence of some tumour types in dogs and cats.	histologically confirmed inadequate treatment resulted in a lower than expected recurrence percentage in dogs with haemangiopericytoma (38%) and mastocytoma (30%). clinical suspicion of inadequate tumour treatment did not always correlate with the histologically assessed inadequacy, nor with the appearance of local recurrence. local recurrence did not seem to be correlated with histological grade of malignancy and tumour size. local injection of c. parvum vaccine did not result in a lower percen ...	1987	3118558
induction of interleukin 1 secretion and of tumoricidal activity in macrophages are not closely related phenomena.	rat bone marrow-derived mononuclear phagocytes (bmm phi), induced to differentiate in vitro and homogeneous with respect to the cell lineage, were interacted with various macrophage-activating agents. the effect of these agents on the secretion of interleukin 1 (il 1) activity and on the development of tumoricidal capacity was comparatively assessed. the findings show that il 1 secretion is considerably enhanced by macrophage-activating factor and by recombinant interferon-gamma but remained una ...	1987	3119353
a study on the adhesive properties of cor. diphtheriae and cor. parvum in the direct haemagglutination reaction.	the adhesive properties of one cor. parvum and two cor. diphtheriae strains were investigated by means of the direct haemagglutination reaction (har). using a specifically developed har procedure, it was proved beyond doubt that the studied corynebacteria possess adhesive properties apparently associated with the presence of pili (fimbrias) on their surfaces. in addition, a correlation was found between the agglutinating activity of bacteria and the conditions of culture growth. based on the rep ...	1987	3119703
propionibacterium acnes: a neglected head and neck pathogen.		1987	3120131
cytopathogenic protein in filtrates from cultures of propionibacterium acnes isolated from patients with kawasaki disease.	propionibacterium acnes may have a role in kawasaki disease. filtrates from cultures of p acnes isolated from cervical lymph node biopsy specimens and blood samples from patients with kawasaki disease were studied and compared with samples from control subjects. after inoculation of human embryo liver cells with filtrates from the patients a cytopathogenic effect and vacuolation were seen. a specific cytopathogenic substance was found in only the filtrates of cultures from patients with kawasaki ...	1987	3120957
modulation of beta-galactosidase activity in peritoneal macrophages from c57b1 mice after exposure to proprionibacterium acnes.	peritoneal macrophages (pm luminal diameter) from untreated c57b1 mice contain high levels of beta-galactosidase (beta-gal) and these pm luminal diameter are heterogeneous in their expression of this enzyme. intraperitoneal (i.p.) injection of saline caused a transient depression in the level of enzyme activity in the pm luminal diameter whereas i.p. injection of proprionibacterium acnes (p. acnes) gave rise to a marked decrease of beta-gal activity in these cells. this reduction in enzymatic ac ...	1987	3122521
the role of hepatic lectins and the activity of the mononuclear phagocyte system in systemic listeria monocytogenes infection in balb/c mice.	hepatic lectin blocking experiments with d-galactose in balb/c mice showed that parenchymal liver cells are obviously not involved in listeria monocytogenes infection (strain slcc 4013, 5 x 10(6) cells i.v.). using the bacterial immunomodifier propionibacterium avidum kp-40 the importance of an activated mononuclear phagocyte system in the early stage of listeria infection could be demonstrated.	1988	3123903
contribution of the microflora to proteolysis in the human large intestine.	protease activities in human ileal effluent were approximately 20-fold greater than in normal faeces. comparative studies with faeces from a person who did not have a pancreas suggested that a substantial proportion of the proteolytic activity in normal faeces was of bacterial origin. thimerosal, iodoacetate, edta and cysteine significantly inhibited proteolysis in faeces, but not in small intestinal contents, showing that cysteine and metalloproteases were produced by bacteria in the large gut. ...	1988	3127369
antitumor effect of normal intestinal microflora on ehrlich ascites tumor.	in order to investigate the antitumor activity of intestinal microflora, the constitution of normal flora was examined in humans, guinea pigs and mice. it was clarified that eubacterium, bifidobacterium and bacteroides were the predominant bacterial genera in humans. in addition, neither clostridium nor enterobacteriaceae was detected in guinea pigs and neither clostridium nor bifidobacterium was present in mice. total bacterial counts in tumor-bearing mice were reduced in comparison with those ...	1988	3128498
the influence of adjuvant on induction of protective immunity by a non-living vaccine against schistosomiasis.	mice were protected against subsequent infection with schistosoma mansoni by intradermal or s.c. vaccination with killed schistosomula or soluble parasite extracts and bacillus calmette-guérin (bcg). treatment with i.p. immunization was somewhat less effective, whereas i.m. vaccination failed to elicit protective immunity. the level of resistance induced by intradermal immunization was influenced by the strain of bcg used, and isolated bcg cell walls did not reliably substitute for whole bcg org ...	1988	3128608
natural production and release of tumour necrosis factor.	tumour necrosis factor (tnf) was first described as an oncolytic factor found in sera of animals injected (primed) with reticuloendothelial stimulators and subsequently (days later) given lipopolysaccharide (lps). tnf is not found in the serum of 'primed' animals but can be found in animals given lps alone when sensitive assays are employed. tnf appears almost immediately upon lps injection, reaches a maximum from about 1.5-2 hours and disappears rapidly thereafter, and is almost undetectable by ...	1987	3131075
effect of mutations at met-88 and met-90 on the biotination of lys-89 of the apo 1.3s subunit of transcarboxylase.	the apo 1.3s subunit of transcarboxylase contains the sequence ala-87-met-88-lys-89-met-90, and it is lys-89 that is biotinated. this sequence is highly conserved in all the biotin enzymes that have been sequenced (with the exception of acetyl-coa carboxylase from chicken liver, which has val in place of ala). the role of met-88 and met-90 in specifying lys-89 for biotination by synthetase was examined by site-directed mutagenesis. genes of the 1.3s subunit coding for thr-88, leu-88, or leu-90 w ...	1988	3131174
antimicrobial properties of kupffer cells.	to characterize the antimicrobial activities of kupffer cells, i harvested macrophages from livers with a technique involving perfusion with collagenase and dnase. ninety-nine percent of glass-adherent cells had typical macrophage morphology, 99% were esterase positive, and 60% phagocytosed opsonized zymosan when challenged with four particles per macrophage. toxoplasma gondii multiplied within kupffer cells from unmanipulated mice, but multiplication was intermediate between that observed in hi ...	1988	3131244
prosthetic valve endocarditis caused by propionibacterium acnes.		1988	3132285
expression of the biotin biosynthetic operon of escherichia coli is regulated by the rate of protein biotination.	in escherichia coli biotin biosynthesis is repressed by high concentrations of exogenous biotin. this paper reports that upon high level production of the apo form of a biotinated protein, biotin operon expression was derepressed by 8-10-fold. the biotinated protein studied was the 1.3 s subunit of propionibacterium shermanii, and transcarboxylase derepression was assayed by beta-galactosidase production in strains which carry a lacz gene altered such that it is transcribed from biotin operon pr ...	1988	3134346
protective effects of a leukotriene inhibitor in an experimental massive hepatic cell necrosis model.	when heat-killed propionibacterium acnes was intravenously injected into mice and seven days later, a small amount of gram-negative lipopolysaccharide was also intravenously injected, most of them died of massive hepatic cell necrosis. however, when azelastine hydrochloride, a leukotriene antagonist chemically known as 1(2h)-phthalazinone, 4-[(4-chlorophenyl)methyl]-2-(hexahydro-1-methyl-1h-azepine-4-yl)-, monohydrochloride, or aa861, 2-(12-hydroxydodeca-5, 10-diynyl)-3,5,6-trimethyl-1, 4-benzoq ...	1988	3136049
alteration of non-specific resistance to infection with listeria monocytogenes.	the experimental infection of murine hosts with listeria monocytogenes is often used as a model for cell-mediated immunity. however, the natural immunity or non-specific resistance to listeriosis can be influenced by the parasite itself and also by a wide array of endogenous and exogenous host factors. the most important host factor in inbred mouse strains is their genetically determined susceptibility or resistance to listeria monocytogenes. secondly, the age of the mice is crucial for the outc ...	1988	3138182
effect of psk, a protein-bound polysaccharide from coriolus versicolor, on drug-metabolizing enzymes in sarcoma-180 bearing and normal mice.	the effects of psk and propionibacterium acnes (anaerobic corynebacterium) on hepatic drug-metabolizing enzymes were studied using sarcoma-180 bearing and non-tumor bearing mice. psk had no influence on aminopyrine n-demethylase and aniline hydroxylase activities, cytochrome p-450 concentration in hepatic microsomes, and the reductase activity of cytochrome c in normal mice. the content of cytochrome p-450 was not significantly reduced in s-180 bearing mice. on the other hand, p. acnes administr ...	1988	3139576
effect of the application of an anionic detergent combined with fabry's tincture and its components on human skin resident flora. part 1: dermofug solution combined with either fabry's tincture or 50 v/v% isopropanol.	the aim of the study was to investigate the antimicrobial longterm efficacy of the combined application of an anionic detergent (dermofug) either with 50 v/v% isopropanol or fabry's tincture (3 w/v% salicylic acid, 1 v/v% liquefied phenol containing 50 v/v% isopropanol) on the resident flora of the human forehead skin after repeated applications using the forehead skin test. the test results show that both combinations tested are able to penetrate the infrainfundibulum of the sebaceous glands. t ...	1988	3142169
effect of the application of an anionic detergent combined with fabry's tincture and its components on human skin resident flora. part 2: dermofug solution combined with either salicylic acid tincture or phenol acid tincture.	using the forehead skin test, we investigated the combined application of 10 v/v% dermofug solution and 3 w/v% salicylic acid tincture or 1 v/v% phenol acid tincture. the former combination showed better antibacterial efficacy against the coagulase-negative staphylococci and propionibacterium spp. in the stratum corneum/acroinfundibulum as well as in the infrainfundibulum of the sebaceous glands. no antibacterial efficacy could be determined against propionibacterium spp. in the infrainfundibulu ...	1988	3142170
microbial ecology of the skin.	humans exist in an environment replete with microorganisms, yet only a few of these microorganisms become residents on the skin surface. these resident flora and the skin constitute a complex ecosystem in which organisms adapt to changes in the microenvironment and to coactions among microorganisms. the skin possesses an assortment of protective mechanisms to limit colonization, and the survival of organisms on the surface lies in part in the ability of the organisms to resist these mechanisms. ...	1988	3144238
comparison of efficacy of mitomycin-c and corynebacterium parvum in the management of malignant pleural effusion.		1988	3150706
polyacrylamide gel electrophoresis (page) of whole-cell proteins of cutaneous propionibacterium species.	polyacrylamide gel electrophoresis (page) was applied to the study of whole-cell proteins of cutaneous propionibacteria in an attempt to characterise possible protein patterns that may be typical for strains isolated from acne skin. isolates were obtained from the faces of 33 individuals aged 7-16 years. some of these subjects had apparently normal healthy skin, whereas others had acne vulgaris of varying severity. twenty-five facial isolates of propionibacterium acnes and eight of p. granulosum ...	1985	3155801
blepharitis associated with acne rosacea and seborrheic dermatitis.		1985	3156100
induction of tumoricidal activity of polymorphonuclear leukocytes by a linear beta-1,3-d-glucan and other immunomodulators in murine cells.	the cytotoxic activity of polymorphonuclear leukocytes (pmn) against tumor cells induced in vitro by antitumor immunomodulators was examined by a 51cr release cytotoxicity assay. among 28 immunomodulators and other agents thus far tested, only beta-1,3-glucan from alcaligenes faecalis var. myxogenes ifo 13140, bacillus calmette-guérin, propionibacterium acnes, zymosan a, and nocardia cell wall skeleton were found to cause induction. the cytotoxic activity of pmn with the beta-1,3-glucan was very ...	1985	3156669
[effects on the lymphreticular system and inhibition of liver microsomaal cytochrome p-450 in mice administered priopionibacterium acnes (corynebacterium parvum) bacterin].		1985	3157461
acne fulminans with osteolytic lesions.	multifocal lytic bone lesions were found in a patient with a severe form of acne. the clinical presentation was suggestive of "acne fulminans." propionibacterium acnes was isolated from a subculture of bone tissue. the association of acne fulminans and osteolytic lesions is rare and the pathophysiology is unknown. however, the use of corticosteroids for systemic effect combined with local measures seems to give excellent treatment results.	1985	3158287
antibody titers to propionibacterium acnes cell wall carbohydrate in nodulocystic acne patients.	in order to determine which structures in propionibacterium acnes are most antigenic to severe acne patients, we studied the specificity of anti-p. acnes antibodies in serum from 15 nodulocystic acne patients and 5 normals. complement fixation titers to p. acnes cell wall fractions were determined using guinea pig serum as a complement source. the mean titers of patients and normals to whole cells were 39.6 and 3 (p less than 0.1); to crude cell wall, 138 and 8 (p less than 0.01); and to protein ...	1985	3158714
isolation of an acidic polysaccharide antigen from propionibacterium acnes.	an acidic polysaccharide antigen is released from propionibacterium acnes i and ii during growth. the molecular weight of the antigen was heterogeneous and when fractionated on a sepharose cl-6b column, the antigen was detected at kav values of between 0.1 and 1.0. the pi of the antigen was below 3.5. rabbit antiserum raised against purified acidic-polysaccharide agglutinated p. acnes showing that the polysaccharide was a surface antigen. human antibodies towards whole p. acnes-ii organisms were ...	1985	3160311
the pathological and ecological significance of microorganisms colonizing acne vulgaris comedones.	a microbiological survey has been undertaken of comedones isolated by micro-dissection from skin biopsies. of closed comedones 10.7% and of open comedones 7.1% did not contain pityrosporum spp., propionibacterium spp. or staphylococcus spp., the organisms most frequently associated with the pathogenesis of acne. mature comedones were more frequently colonised than were young comedones. these results support the argument that the presence of microorganisms is not a prerequisite for comedo formati ...	1985	3160861
inhibition of bacterial translocation from the gastrointestinal tract by nonspecific immunostimulation.		1985	3161085
cytotoxic activity of propionibacterium acnes and other skin organisms.	culture supernatants from four species of skin micro-organisms were tested against vero (monkey kidney cells) and skin fibroblasts for cytotoxic activity. cytotoxic activity was produced by the three species of propionibacterium tested (p. acnes, p. avidum and p. granulosum), and this activity was highest when cultures were grown in the presence of glucose. in contrast, staphylococcus epidermidis was devoid of cytotoxic activity whether grown in the presence of glucose or not. the agent responsi ...	1985	3161535
acne-like chronic inflammatory activity of propionibacterium acnes preparations in an animal model: correlation with ability to stimulate the reticuloendothelial system.	the ability of strains and fractions of killed propionibacteria suspensions to produce chronic rat ear inflammation after intradermal injection of 70-micrograms aliquots was highly correlated with production of splenomegaly in the mouse after i.p. injection of 1.4 mg propionibacterium acnes strains cn 6134, vpi 0009, atcc 11828, and ucla sc and n1 produced a 2- to 3-fold increase in rat ear thickness and a 5- to 7-fold increase in mouse spleen weight 15 days post injection. in contrast p. granul ...	1985	3161957
[the use of corynebacterium parvum in pleurodesis].		1988	3168834
ocular dangers in the garden. a new menace--nylon line lawn trimmers.	nylon line lawn trimmers represent a source of potentially devastating ocular trauma. five cases of ocular trauma associated with the use of line trimmers are reported. these case reports document new mechanisms and circumstances of injury, including trauma from debris other than nylon line fragments and injury to bystanders. furthermore, injuries are often serious, with two of these patients requiring an evisceration or enucleation after endophthalmitis developed. in one patient, six different ...	1988	3174040
effects of the polysaccharide chain of lipopolysaccharide in an experimental massive hepatic cell necrosis model.	when small amounts (1 microgram) of lipopolysaccharide (lps) purified from salmonella minnesota (sm) wild, sm r60 and sm r345 were intravenously injected into mice 7 days after heat-killed propionibacterium acnes was intravenously injected, massive hepatic cell necrosis was induced and most of the mice died within 24 hours. however, when lps from sm r345 treated galactosidase, sm r5 and sm r7 and lipid a from sm r595 were administered, the survival rate was much higher and no histological change ...	1988	3178810
effects of the polysaccharide chain of lipopolysaccharide in an experimental massive hepatic cell necrosis model in the mice.	when a small amount (1 microgram) of lipopolysaccharide (lps) purified from salmonella minnesota wild, salmonella minnesota r60, and salmonella minnesota r345 was intravenously injected into mice 7 days after heat-killed propionibacterium acnes was intravenously injected, massive hepatic cell necrosis was induced and most of the mice died within 24 hours. however, when lps from salmonella minnesota r5 and salmonella minnesota r7 and lipid a from salmonella minnesota r595 were administered, the s ...	1988	3181665
bacteriological findings of acute maxillary sinusitis in young adults.	bacteriological findings in 339 sinus secretions obtained by puncture were investigated in 238 young adult patients with acute maxillary sinusitis. aerobic and anaerobic cultures were performed immediately. a total of 76% of the secretions were positive. the most common pathogens isolated were haemophilus influenzae (50%), streptococcus pneumoniae (19%), streptococcus pyogenes (5%), and branhamella catarrhalis (2%). coagulase-negative staphylococci and staphylococcus aureus were isolated in 8 an ...	1988	3182986
corynebacterium parvum as palliative treatment in malignant ascites.		1988	3183831
application of electroporation for transfer of plasmid dna to lactobacillus, lactococcus, leuconostoc, listeria, pediococcus, bacillus, staphylococcus, enterococcus and propionibacterium.	plasmid dna was introduced by electroporation into bacillus, enterococcus, lactobacillus, lactococcus, leuconostoc, listeria, pediococcus, propionibacterium and staphylococcus as an alternative to competent-cell or protoplast transformation. plasmid-containing transformants were recovered in these recipients at frequencies ranging from 10(1) to 10(5) transformants micrograms-1 of pgk12. several parameters of the protocol, including dna concentration, voltage, plating regimen and electroporation ...	1988	3185282
role of anaerobic bacteria in aortofemoral graft infection.	a 10-year review of specimens of aortofemoral graft infection sent to the microbiology laboratory for cultures of anaerobic bacteria revealed the presence of these organisms in 13 of 16 specimens. nineteen organisms (1.5 per patient) were isolated: 16 anaerobes and 3 aerobes. the predominant bacteria were anaerobic gram-positive cocci (six isolates), propionibacterium acnes (five), and bacteroides fragilis group (four). polymicrobial infection was present in three patients, all with decubitus ul ...	1988	3187899
involvement of tryptophans at the catalytic and subunit-binding domains of transcarboxylase.	transcarboxylase from propionibacterium shermanii is a multisubunit enzyme. it consists of one central hexameric subunit to which six outer dimeric subunits are attached through twelve biotinyl subunits. both the central and the outer subunits are multi-tryptophan (trp) proteins, and each contains 5 trps per monomer. the roles of the trps during catalysis and assembly of the enzyme have been studied by using n-bromosuccinimide (nbs) oxidation as a probe. modification of approximately 10 trps of ...	1988	3191102
involvement and identification of a tryptophanyl residue at the pyruvate binding site of transcarboxylase.	transcarboxylase (tc) from propionibacterium shermanii consists of a central hexameric 12s subunit to which 6 outer dimeric 5s subunits are attached through 12 biotinyl 1.3s subunits. the enzyme catalyzes the transfer of a carboxyl group from methylmalonyl-coa to pyruvate, forming oxalacetate and propionyl-coa. the pyruvate binding site, located on the 5s subunit, was examined by monitoring the intrinsic fluorescence quenching accompanying the incremental addition of pyruvate to either tc or the ...	1988	3191103
tumor necrosis factor/cachectin. induction of hemorrhagic necrosis in normal tissue requires the fifth component of complement (c5).	tnf induces hemorrhagic necrosis (hn) when injected into skin exposed to bacterial agents but not when injected into normal skin. in this paper, we present several lines of evidence suggesting that tnf requires the fifth component of complement (c5) to induce hn in skin exposed to bacteria. first, mouse strains that do not have c5 did not develop hn after injection of tnf and bacteria into skin. second, plasma from c5-sufficient mice could correct the defect in these c5-deficient mice. third, he ...	1988	3199066
in vitro activity of flomoxef compared to moxalactam, cefoxitin, cefotaxime, and clindamycin against anaerobes.	to assess the in vitro activity of flomoxef (6315-s), moxalactam, cefoxitin, cefotaxime, and clindamycin against anaerobes 197 clinical isolates (27 bacteroides fragilis, 42 b. thetaiotaomicron, 10 b. vulgatus, 7 b. ovatus, 6 b. uniformis, 6 b. distasonis, 7 bacteroides melaninogenicus group, 11 bacteroides oralis group, 21 clostridium difficile, 7 c. perfringens, 3 c. sporogenes, 3 clostridium spp., 33 propionibacterium acnes, 14 peptococcaceae) were studied by means of agar dilution tests. the ...	1988	3214438
studies on a biological response modifying lysat from propionibacterium avidum kp 40.	propionibacterium avidum kp 40 cells were mechanically disintegrated in order to obtain the soluble immunostimulatory (antineoplastic) lysat. chemiluminescence measurements of human leukocyte function yielded enhanced activation of the cells after incubation with 2.5 and 5 mg of lysat. as compared to non-treated controls, administration of lysat to balb/c-mice (1, 2.5 and 5 mg; intraperitoneally, subcutaneously, intranasal; 7, 4 and 2 days prior to challenge) induced a significant enlargement of ...	1988	3223140
age and lpr dependent induction of increased sensitivity to the toxic effects of lipopolysaccharide and indomethacin in mrl mice: evidence for res activation during disease progression.	injection of 4.5-6 month old female mrl-lpr/lpr mice with 200-250 micrograms lipopolysaccharide led to the death of the animals within two days. treatment of age matched female control mice (mrl-+/+) or young (3 month) mrl-lpr/lpr mice did not lead to similar toxicity. however, pretreatment of young mice with the pyridine extract of residue of corynebacterium parvum (alternatively designated propionibacterium acnes) prior to lipopolysaccharide injection, led to the rapid death of 100% of the mic ...	1988	3230580
properties and characteristics of a bacteriocin-like substance produced by propionibacterium acnes isolated from dental plaque.	a cell-associated bacteriocinlike substance with an approximate molecular weight of 78,000 was isolated from an oral isolate of propionibacterium acnes. the substance was bacteriostatic and was active against both gram-positive and gram-negative anaerobes. lysozyme inhibited the activity of the bacteriocinlike substance at low ionic strength.	1988	3233559
characteristics of the hepatocytotoxic factor in mice with experimentally-induced massive hepatic cell necrosis.		1988	3248030
endophthalmitis following nd:yag laser posterior capsulotomy.	we report the development of propionibacterium acnes endophthalmitis following nd:yag laser posterior capsulotomy. the patient previously underwent uncomplicated extracapsular cataract extraction with intraocular lens insertion and was free of inflammation prior to laser capsulotomy. diagnostic vitrectomy and aqueous tap were performed, and p. acnes was isolated from the aqueous in thiol broth media after nine days of incubation under anaerobic conditions. the patient was managed with topical an ...	1988	3258419
propionibacterium acnes.		1988	3261615
intravitreal liposome-encapsulated drugs: a preliminary human report.	intravitreal liposome-encapsulated antibiotics and antiviral drugs were used in patients with acute toxoplasmosis retinochoroiditis, presumed propionibacterium acne endophthalmitis after cataract surgery, and presumed cytomegalovirus retinitis associated with aids. a single intravitreal dose was effective in the treatment of all the conditions. intravitreal liposomes may prove to be an advantageous drug delivery system for the treatment of chronic intraocular inflammatory disorders.	1988	3265937
eradication of syngeneic tumor (meth a fibrosarcoma) from mice by adoptive immunotherapy of immunized spleen cells induced by corynebacterium parvum-pyridine extract residue.	eradication of immunologically-syngeneic tumors was achieved by adoptive chemotherapy using effector cells induced by corynebacterium parvum-pyridine extract residue (cp-per). a mixture of 2 x 10(6) meth a cells and 0.1 mg cp-per was subcutaneously inoculated into the back of donor balb/c mice, with the result that their spleen cells showed an antitumor effect 10 to 13 days after the inoculation. these cells were used as immune cells. recipient mice were inoculated with 1 x 10(6) meth a cells, a ...	1988	3266420
[in vitro experiments with respect to the invasiveness of propionibacterium acnes into human teeth].		1988	3269190
tissue localization and kinetics of pit cells or large granule lymphocytes in the liver of rats treated with biological response modifiers.	the numbers of kupffer cells (macrophages) and pit cells (large granular lymphocytes) were counted by light and electron microscopy in perfusion-fixed liver sinusoids. after a single intravenous injection of the biological response modifiers zymosan, propionibacterium acnes and ok-432, a 4- to 6-fold increase in the number of pit cells and a 2- to 4-fold increase in the number of kupffer cells were observed within a period of 4 to 7 days. the mechanisms of the pit cell accumulation were further ...	1988	3276590
antitumour effects of streptococcal lipoteichoic acids on meth a fibrosarcoma.	the antitumour effects of lipoteichoic acids (lta) extracted from streptococcus pyogenes were studied in comparison with other streptococcal cellular components. lta suppressed the tumour growth of both solid- and ascites-type meth a fibrosarcoma as did the whole cells of s. pyogenes (ok-432). no other cellular components, such as cell wall peptidoglycan, group-specific c-carbohydrate or type-specific m protein, suppressed the growth of meth a. lta, but not the other cellular components, induced ...	1988	3279996
[effect of propionibacterium granulosum kp-45 on the resistance to listeria monocytogenes and salmonella typhimurium infections].	killed bacterial cells of propionibacterium granulosum kp-45 were found to be potent modifiers of non-specific resistance of mice against infection with either listeria monocytogenes or salmonella typhimurium. injection of p. granulosum 7 days prior to infection resulted in marked splenomegaly. disseminated acute inflammatory foci were found histologically in the liver. these mice were apparently less susceptible to infection, since definitely lower bacterial counts were determined. simultaneous ...	1988	3287804
phototoxicity of skin microorganisms tested with a new model.	a new standardized method for testing phototoxicity of chemicals against microorganisms is described. the inoculum size of the microorganism, application of test chemicals, prediffusion time, incubation time and incubation period are defined. staphylococcus aureus, s. epidermidis, candida albicans, and pityrosporum orbiculare were studied. both 8-methoxypsoralen and trimethylpsoralen were phototoxic against all microorganisms tested, while tetracycline and doxycycline were not phototoxic. c. alb ...	1988	3288127
[preoperative nonspecific immunostimulation with propionibacterium granulosum kp-45 in patients with colorectal cancer. a prospective randomized study].	the effect of preoperative unspecific immunomodulation with propionibacterium granulosum kp-45 (p.g.) on postoperative complications and patient survival was evaluated in a prospectively randomized trial in patients with colorectal tumor resection. patients of the therapy group (n = 51) received at least 3 days before operation an infusion of 10 mg p.g., patients of the control group (n = 49) were not treated at all. the wound infection rate and the number of re-explorations were significantly l ...	1988	3289850
treatment of malignant pleural effusions with intracavitary corynebacterium parvum.	fifty-three cases of metastatic pleural effusion (30 haemorragic and 23 serofibrinous) were treated with 4 mg of corynebacterium parvum (cp) injected weekly into the pleural cavity after total thoracentesis. of the 53 effusions, 24 were metastases from lung cancer and 29 from breast cancer. complete response (cr) was assessed as total resolution of pleural effusion after explorative thoracentesis. the results were as follows: 15 cr after two injections of cp, 30 cr after three, and 5 cr after th ...	1988	3293763
corynebacterium parvum, but not bcg, induces elevations in plasma proteinase activity similar to those observed in tumor-bearing mice.	intraperitoneal administration of corynebacterium parvum to balb/c, c57bl/6 or c3h/hej mice lead to the induction of elevated levels of neutral proteinase activity (125i-caseinolytic activity) similar to those observed previously in animals bearing the bcl1 leukemia or the b16-f10 melanoma. enhanced activity reached a peak at 7-14 days postinjection of the c. parvum and then gradually returned to normal levels by 20-25 days postinjection. increased plasma proteinase activity could be induced by ...	1987	3297943
effects of radiation and other influences on chemical lymphomagenesis.	methylnitrosourea (mnu) or butylnitrosourea (bnu) was used to induce t cell lymphomas (thymomas) in bdf1 mice. in addition to the chemical, x-rays in various dose schedules were applied. an effect of the irradiation (shortening of the latency period) was seen with 12 x 0.25 gy in protocols with a prolonged median induction time in the controls as a result of a dose reduction of the chemical (median induction time 27-36 weeks instead of 16-18 weeks under 'optimal' conditions using 50 mg kg-1 of m ...	1987	3298104
biofilms on right heart flow-directed catheters.	this study was designed to detect biofilm and bacteria on right heart flow-directed catheters using scanning electron microscopy and culture following scraping and dispersion of biofilm by sonication. we examined 20 consecutive catheters removed from 18 critically ill patients, an average of 2.6 days after insertion. on scanning electron microscopy, all catheters were found to be covered by a biofilm, with bacteria visible on 50 percent of them. cultures of specimens from 40 percent of the cathe ...	1987	3304847
susceptibility in vitro of gram-positive aerobe and anaerobe bacteria to ofloxacin.	ofloxacin is a new fluoroquinolone derivative active against gram-positive and gram-negative bacteria including obligate anaerobes. in this study the in vitro activity of ofloxacin was evaluated against 325 gram-positive organisms freshly isolated from clinical specimens, in comparison with that of ampicillin, erythromycin, clindamycin and ceftazidime. susceptibility tests indicated that the mic90 was 2 mg/l (range 0.25-2) against both methicillin-susceptible (ms) and methicillin-resistant (mr) ...	1987	3304918
fate of legionella pneumophila philadelphia-1 strain in resident, elicited, activated, and immune peritoneal macrophages of guinea pigs.	legionella pneumophila is known to grow intracellularly in resident peritoneal macrophages of guinea pigs. the present study was done to determine what kinds of macrophage stimulants are able to activate guinea pig macrophages to inhibit intracellular growth of the organism. peritoneal macrophages were harvested from healthy guinea pigs, from guinea pigs injected intraperitoneally with proteose peptone (pp) or thioglycolate medium, from guinea pigs injected intraperitoneally with live mycobacter ...	1987	3308708
regulatory interactions between macrophages and t cells in mycobacterium lepraemurium-specific t-cell activation.	the antigen-specific proliferative response of draining lymph node cells was found to follow a similar pattern in both c57bl and balb/c mice following subcutaneous infection with mycobacterium lepraemurium (mlm), although the two strains differed in their ability to control bacterial growth at the site of infection. the proliferative response, which was maximal 1-2 weeks postinfection, was t-cell dependent as it was abrogated with anti-thy 1.2 + c treatment. the response was also abrogated by pr ...	1987	3319191
steroids and tuftsin fail to prevent the induction of altered plasma proteinase homeostasis in mice bearing the b16 melanoma or treated with c. parvum.	the effects of tuftsin and steroids (methyl prednisolone) on the induction of disrupted plasma proteinase regulation in mice bearing the b16 melanoma or treated with corynebacterium parvum was investigated. tuftsin treatment inhibited tumor progression only if treatment was started at the time of tumor transplantation. however, tuftsin inhibited the development of splenomegaly in mice with established tumors. in contrast, tuftsin did not influence either the induction of elevated plasma proteina ...	1987	3319918
immune complex study of biopsy specimens from kawasaki disease patients.		1987	3321077
changes in expression of bacterial surface antigens induced by antibiotics and their influence on host defenses.	concomitant with the discovery that a number of antibiotics were able to induce profound morphological changes (increased septation, thickened walls, filamentation) at low concentrations their ability to inhibit bacterial protein/polysaccharide synthesis was determined. in particular toxin and extracellular enzyme biosynthesis could be repressed by the lincosamines, fusidic acid, erythromycin and streptomycin in staphylococcus aureus, streptococcus pyogenes, propionibacterium acnes, pseudomonas ...	1987	3325899
prevention of peroral and congenital acquisition of toxoplasma gondii by antibody and activated macrophages.	intramuscular administration of toxoplasma gondii lysate antigens to mice produced titers of t. gondii-specific antibody (measured by sabin-feldman dye test) greater than or equal to 1:1,024 in their sera. intravenous administration of heat-killed propionibacterium acnes to mice produced peritoneal macrophages with enhanced microbicidal capacity against t. gondii. mice with high antibody titers or activated peritoneal macrophages or both had reduced numbers of toxoplasma cysts in their brains 30 ...	1988	3335411
studies on the endogenous flora of the human breast.	identification of the endogenous microbiological flora of the human breast and its role in breast infections following subglandular augmentation or reduction mammaplasty was undertaken. a total of 231 cultures were performed on 59 breasts in 30 patients. patients were followed for 12 months. no fungus was cultured from any specimen. of the breasts cultured 53% were positive for coagulase-negative staphylococcus. other aerobes found included diphtheroids, lactobacillus, d-enterococcus, micrococcu ...	1988	3341714
experimental immunological intrahepatic cholestasis model.	heat-killed propionibacterium acnes (p. acnes) was intravenously injected into tuberculin-sensitized guinea pigs, and 7 days later, purified protein derivative (ppd) was also injected intravenously resulting in marked reductions in bile flow and bile acid excretion. serum levels of bile acids and cholesterol were increased, and the activities of alkaline phosphatase (alk-p) and leucine aminopeptidase (lap) were also elevated. histological examination revealed dilatation of bile canaliculi and di ...	1988	3350277
aerobic and anaerobic bacteriology of purulent nasopharyngitis in children.	cultures of aerobic and anaerobic bacteria were obtained from the inferior nasal meatus of 25 children with purulent nasopharyngitis and from 25 controls. microorganisms were isolated from all specimens. a total of 98 isolates (3.9 per patient), 45 aerobes (1.8 per patient) and 53 anaerobes (2.1 per patient), were isolated in patients with purulent nasopharyngitis. seventy-three isolates (2.9 per patient), 47 aerobes (1.9 per patient) and 26 anaerobes (1.0 per patient) were found in the controls ...	1988	3356794
propionibacterium acnes lens abscess after traumatic implantation of intralenticular cilia.		1988	3358438
treatment of chronic sinusitis with open drainage and cefaclor.	a prospective study of 50 adult patients with chronic sinusitis was undertaken. forty patients were treated with the caldwell-luc operation and postoperative cefaclor, and 10 underwent nasal antrostomy and treatment with cefaclor. approximately 85% of the 40 patients had resolution of symptoms with this treatment. the microbiology of chronic sinusitis was studied through aerobic and anaerobic cultures of sinus secretions obtained at surgery. staphylococcus aureus (coagulase-negative) and propion ...	1988	3358484
purification and properties of the synthetase catalyzing the biotination of the aposubunit of transcarboxylase from propionibacterium shermanii.	the synthetase that attaches biotin to the aposubunit of transcarboxylase (biotin-[methylmalonyl-coa-carboxyltransferase]ligase) (ec 6.3.4.9) was purified to homogeneity by ion-exchange chromatography on cellulose de-52 and cm-cellulose. the synthetase is a monomer of molecular weight 30,000. the ph and temperature optima for the synthetase are 6.0 and 37 degrees c, respectively. the apparent km for the substrates atp, biotin, and apo 1.3 s subunit of apotranscarboxylase are 38, 2.0, and 0.9 mic ...	1988	3360240
correction of defective tumoricidal activity of macrophages from a/j mice by liposomal immunomodulators.	the ability of liposomal immunomodulators to restore abnormal macrophage tumoricidal activity has been studied. macrophages from a/j mice have impaired responses in vitro to macrophage activating factor, gamma-interferon, n-acetylmuramyl-l-alanyl-d-isoglutamine (mdp) or lipopolysaccharide when compared with macrophages from normoresponsive c57bl/6j mice. liposomes containing a lipophilic muramyl dipeptide mdp-glyceroyl dipalmitate, macrophage activating factor or gamma-interferon restored tumori ...	1988	3360595
neutrophil involvement in effects of diethylstilbestrol and strontium 89 on macrophage activation by propionibacterium acnes.	we have recently demonstrated that diethylstilbestrol (des) significantly suppresses macrophage (m phi) activation by propionibacterium acnes. because the initial activation of m phi by p. acnes appears to involve the close interaction of the killed bacteria with inflammatory neutrophils (pmn) and resident m phi in the peritoneal cavity, we investigated whether the des inhibition of m phi activation was associated with inhibition of the pmn response. our data demonstrate that treatment of mice w ...	1988	3366512
susceptibility of anaerobic bacteria to antimicrobial agents.	the antimicrobial susceptibility of 1,117 clinical isolates of anaerobic bacteria was determined by the agar dilution technique. metronidazole was the most active agent; only propionibacterium acnes and actinomyces sp. isolates were resistant. clindamycin and chloramphenical were the next most effective agents. beta-lactam antibiotics, with the exception of penicillin, were active against most anaerobes other than the bacteroides fragilis group. at a breakpoint of 8 mg/l, 25% of fusobacterium sp ...	1988	3374973
liver injury model in mice for immunopharmacological study.	experimental liver injury was produced in mice by the immunological technique. the utility of these models as an immunopharmacological method was investigated. the first model was produced by the injection of anti-basic liver protein (blp) rabbit antibody into dba/2 mice that had been previously immunized with rabbit igg. the second liver injury was caused by injection of anti-liver specific protein (lsp) rabbit antibody into dba/2 mice. the third model was produced by the injection of bacterial ...	1988	3379835
cecal and fecal bacterial flora of the mongolian gerbil and the chinchilla.	the mongolian gerbil is being increasingly used as a laboratory animal and as a pet. both chinchillas and gerbils are used as animal models for otitis media and other otic research. previously, only incomplete information was available regarding the indigenous bacterial flora of the lower intestinal tracts of these coprophagic animals. using the strict anaerobic methodology of the virginia polytechnic institute anaerobe laboratory, we studied the predominant bacterial flora of the cecum and feca ...	1988	3389814
the ability of inflammatory bronchoalveolar leucocyte populations elicited with microbes or mineral dust to injure alveolar epithelial cells and degrade extracellular matrix in vitro.	inflammatory cells are recruited to the parenchyma of the lung in a range of conditions where they are considered to have the ability to exert damaging effects on elements of the alveolus. the injurious effects of rat bronchoalveolar-derived inflammatory cells on an alveolar type ii epithelial cell line were therefore assessed. inflammatory populations produced by intratracheal injection of corynebacterium parvum or quartz caused non-lethal detachment injury to the epithelial cells on co-culture ...	1988	3390385
amnionitis caused by propionibacterium acnes. case report.		1988	3390407
conjunctival anaerobic and aerobic bacterial flora in paediatric versus adult subjects.	although the bacterial flora of the conjunctiva in children and adults has been studied, there has been no previous comparison between these two age groups of anaerobic as well as aerobic bacteria. conjunctival cultures from 229 eyes of 144 uninfected subjects were analysed for aerobic, microaerophilic, and anaerobic bacteria. adults showed a greater number of species per eye than did younger subjects (1.47 versus 1.13; p less than 0.05). anaerobic species, predominantly propionibacterium, were ...	1988	3390421
the incidence of bacteremia in skin surgery of the head and neck.	the normal microflora of skin vary significantly between sebaceous rich, wet, and dry areas. this would be expected to influence the likelihood of developing transient bacteremia while undergoing skin surgery on these different areas, thereby affecting the risk of infective endocarditis from such procedures. we evaluated the incidence of transient bacteremia in 45 patients undergoing skin surgery on the sebaceous rich areas of the head and neck. after surveillance skin cultures, aerobic and anae ...	1988	3403731
[propionibacterium acnes endocarditis on a natural valve].		1988	3406481
beneficial response of local immunotherapy with propionibacterium granulosum kp-45 in combined treatment of inflammatory breast carcinoma.	nine 44- to 67-year-old patients with inflammatory breast carcinoma were treated over 2 weeks with intratumoral injections of propionibacterium granulosum kp-45 (kp). this period of immunotherapy was succeeded by four courses of chemoimmunotherapy (fac: 5-fluorouracil, adriamycin, and cyclophosphamide + intratumoral kp). inflammatory symptoms disappeared in three patients during immunotherapy and in the remaining six patients during the following chemoimmunotherapy. finally, 3 to 4 months after ...	1988	3410883
isolation and characterization of mutants of propionibacterium strains.	procedures were developed to isolate and characterize mutants of strains of dairy propionibacteria. these procedures included the construction of minimal defined media to support growth of the strains, optimization of conditions of exposure of the strains to nitrosoguanidine, and identification of the phenotypes of the mutants that were generated. the minimal defined medium contained inorganic salts, adenosine, three vitamins, and sodium lactate as the carbon source, with cysteine, methionine, o ...	1988	3410988
phagocytic and natural killer cytotoxic responses of murine transitional cell carcinoma to postsurgical immunochemotherapy.	postsurgical immunochemotherapy with corynebacterium parvum (cp) and cis-diamminedichloroplatinum (ii) (cddp) was evaluated in mice with transitional cell carcinoma (mbt-2). c3h/he mice were transplanted subcutaneously in the hind limb with 5 x 10(5) tumor cells. ten to 14 days later when the tumor reached a diameter of five to seven mm., it was surgically removed. mice were then randomized into four groups to receive a total of three treatments on days 1, 3 and 5 after surgery: 1) saline (contr ...	1988	3411696
5'-methylbenzimidazolyl-cobamides are the corrinoids from some sulfate-reducing and sulfur-metabolizing bacteria.	the sulfate-reducing bacteria desulfobacterium autotrophicum, desulfobulbus propionicus and archaeoglobus fulgidus (vc-16) and the sulfur-metabolizing archaebacteria desulfurolobus ambivalens and thermoplasma acidophilum were found to contain considerable amounts of corrinoids, that were isolated and crystallized in their co beta-cyano form. in three other sulfur-metabolizing archaebacteria, thermoproteus neutrophilus, pyrodictium occultum and staphylothermus marinus significant amounts of corri ...	1988	3416881
propionibacterium acnes in purulent arthritis: probable cause of iatrogenic infection.		1988	3418633
cytopathogenic protein (cpp) produced by propionibacterium acnes isolated from patients with kawasaki disease.		1987	3423083
prolonged fever after pleural instillation of corynebacterium parvum (coparvax).		1987	3424259
septicemia caused by propionibacterium granulosum in a compromised patient.	a case of septicemia caused by propionibacterium granulosum in a compromised patient is described. the patient responded to ampicillin therapy. detailed antibiotic susceptibility data are presented.	1987	3429632